Rw3G000190

Phospholipase A(1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0005852
Physical Location & Seq
Reverse (-)
256661 .. 261510
4850 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G000190.1

Sequence Viewer

Length: 1338 bp
ATGCTGTGCTTCTGCCCCTGTTTCGACGACAACCACCGCCGGAGTTCCGACGACGTGGACCTGGAGCCGGTCCTGCTCATCTCCGGAATCGGAGGTTCCATTCTAACTTCGAAACGCAAAAAACTGGGCTTCGACACTCGGGTCTGGGTACGGATCCTATTGGCTGACTTAGAATTCAGGAAGAAACTCTGGTCTCTCTATAATCCCAAAACAGGTTATACAGAAACTTTAGACAAAGACAATGAAATTGTTGTCCCAGATGATGATTATGGCCTCTATGCAATTGATATTCTAGACCCGTCCCCGTTTGTGAAATGCATACATTTGAAAGAGGTATACTATTTTCATGATATGATAAATATGCTTGTGGGTTGTGGCTATAAGAAGGGAACCACATTGTTTGGATATGGCTATGATTTCCGACAAAGCAACAGAATTGAAAAGTTAATGGAAGATCTTAAAGTGAAATTGGAAACTGCTTACAAGGCTTCTGGCGGTAGAAAAGTAAATATAATTTCGCATTCAATGGGTGGACTGCTAGTCATGTGTTTTATGTCACTTCATCATGAGGCCTTTTCAAAGTATGTGAGTAAATGGATTTGCATCGGTTGCCCTTTTCAAGGTGCACCAGGATGCATCAATGATTCTCTTTTAACTGGACTGCAGTTTGTTGAAGGCTTGGAAAGCTTTTTTTTTGTATCAAGGTGGACTATGCACCAGCTGTTGGTTGAGTGTCCATCAATCTACGAGATGTTGGCCAATCCAGATTTTAATTGGAAAAAGCAGCCGGAAATTCAGGTTTGGAGGAAGCATTCTAAGGATGGGGAAACTTCTATTGAACTGGAATCTTATGGTCCCGTTCAAAGTATTACTTTGTTTAGAGAAGCATTGAGACATAATGAGCTAACTTATGATGGTAAAACTATAGCTTTGCCATTTAACTTTGCTATTTCCAGATGGGCTGCCGAGACTCGCCAAGTTCTCAACAATGCCAAACTACCAGATGGTGTCTGCTTCTATAACATTTATGGAATATCCTTTGACACACCTTTTGATGTTTGTTATGGCTCGAAGGCATCTCCAATTGAAGACTTGTCTGAAATATGCCATTCAATGCCTCAGTATTCTTATGTGGATGGAGATGAAACAGTTTCTGCTGAATCAGCAAAGGCAGATGGATTTGCTGCAGTTGAAAGAGTAGCAATAGCTGCAAGACACCGGGACCTATTAAGTGACAAAACGGTTTTTCAACATATCAAGAAGTGGTTGGGAGTTGAGCAGAAGGTCAACATACATTCCAAGACATCCAGAGTTTCAGATAAGCCTGTGAGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko00592 ko01100 ko01110 map00564 map00592 map01100 map01110
Pfam Domains
Protein Families
LCAT

Protein Analysis

445

Amino Acids

50.69

Weight (kDa)

6.14

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 67 - 389 4.3e-24 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 140
AccB7I CCANNNNNTGG 1 cut(s) 724
AccI GTMKAC 1 cut(s) 336
AccIII TCCGGA 1 cut(s) 83
AciI CCGC 2 cut(s) 37, 495
AclWI GGATC 2 cut(s) 148, 161
AcoI YGGCCR 1 cut(s) 756
AcsI RAATTY 2 cut(s) 173, 792
AfaI GTAC 1 cut(s) 150
AfiI CCNNNNNNNGG 4 cut(s) 67, 212, 620, 724
AhdI GACNNNNNGTC 1 cut(s) 539
AjiI CACGTC 1 cut(s) 55
AjnI CCWGG 2 cut(s) 60, 628
AluBI AGCT 5 cut(s) 687, 721, 904, 929, 1208
AluI AGCT 5 cut(s) 687, 721, 904, 929, 1208
Alw21I GWGCWC 1 cut(s) 628
Alw26I GTCTC 3 cut(s) 198, 886, 962
Alw44I GTGCAC 1 cut(s) 624
AlwI GGATC 2 cut(s) 148, 161
AlwNI CAGNNNCTG 1 cut(s) 1154
Ama87I CYCGRG 1 cut(s) 138
Aor13HI TCCGGA 1 cut(s) 83
AoxI GGCC 3 cut(s) 271, 570, 756
ApaLI GTGCAC 1 cut(s) 624
ApeKI GCWGC 4 cut(s) 784, 962, 1184, 1208
ApoI RAATTY 2 cut(s) 173, 792
ArsI GACNNNNNNTTYG 2 cut(s) 1034, 1066
AspS9I GGNCC 4 cut(s) 58, 70, 854, 1222
AsuC2I CCSGG 1 cut(s) 1220
AsuII TTCGAA 1 cut(s) 110
AvaI CYCGRG 1 cut(s) 138
AvaII GGWCC 4 cut(s) 58, 70, 854, 1222
BaeGI GKGCMC 1 cut(s) 628
BalI TGGCCA 1 cut(s) 758
BamHI GGATCC 1 cut(s) 153
BbsI GAAGAC 1 cut(s) 1095
Bbv12I GWGCWC 1 cut(s) 628
BbvI GCAGC 4 cut(s) 796, 949, 1171, 1195
BccI CCATC 7 cut(s) 745, 815, 908, 951, 998, 1130, 1169
BciT130I CCWGG 2 cut(s) 62, 630
BcnI CCSGG 1 cut(s) 1220
BcoDI GTCTC 3 cut(s) 198, 886, 962
BfaI CTAG 2 cut(s) 293, 539
BfmI CTRYAG 3 cut(s) 662, 924, 1185
BglII AGATCT 1 cut(s) 454
BisI GCNGC 4 cut(s) 785, 963, 1185, 1209
BlsI GCNGC 4 cut(s) 786, 964, 1186, 1210
Bme1390I CCNGG 3 cut(s) 62, 630, 1220
Bme18I GGWCC 4 cut(s) 58, 70, 854, 1222
BmeRI GACNNNNNGTC 1 cut(s) 539
BmeT110I CYCGRG 1 cut(s) 138
BmgBI CACGTC 1 cut(s) 55
BmgT120I GGNCC 4 cut(s) 58, 70, 854, 1222
BmiI GGNNCC 6 cut(s) 66, 97, 155, 391, 856, 1223
BmrFI CCNGG 3 cut(s) 62, 630, 1220
BmrI ACTGGG 1 cut(s) 134
BmsI GCATC 4 cut(s) 612, 623, 645, 1085
BmuI ACTGGG 1 cut(s) 134
BpiI GAAGAC 1 cut(s) 1095
BpmI CTGGAG 1 cut(s) 83
Bpu14I TTCGAA 1 cut(s) 110
BpuMI CCSGG 1 cut(s) 1220
BsaBI GATNNNNATC 1 cut(s) 602
BsaI GGTCTC 1 cut(s) 198
BsaWI WCCGGW 1 cut(s) 83
Bsc4I CCNNNNNNNGG 4 cut(s) 67, 212, 620, 724
Bse118I RCCGGY 1 cut(s) 67
Bse1I ACTGG 3 cut(s) 129, 661, 846
Bse8I GATNNNNATC 1 cut(s) 602
BseAI TCCGGA 1 cut(s) 83
BseBI CCWGG 2 cut(s) 62, 630
BseGI GGATG 4 cut(s) 638, 826, 1141, 1304
BseJI GATNNNNATC 1 cut(s) 602
BseLI CCNNNNNNNGG 4 cut(s) 67, 212, 620, 724
BseMII CTCAG 1 cut(s) 1133
BseNI ACTGG 3 cut(s) 129, 661, 846
BseSI GKGCMC 1 cut(s) 628
BseXI GCAGC 4 cut(s) 796, 949, 1171, 1195
BshFI GGCC 3 cut(s) 273, 572, 758
BsiHKAI GWGCWC 1 cut(s) 628
BsiHKCI CYCGRG 1 cut(s) 138
BsiSI CCGG 5 cut(s) 40, 68, 84, 788, 1219
BslFI GGGAC 4 cut(s) 239, 286, 840, 1235
BslI CCNNNNNNNGG 4 cut(s) 67, 212, 620, 724
BsmAI GTCTC 3 cut(s) 198, 886, 962
BsmFI GGGAC 4 cut(s) 239, 286, 840, 1235
BsmI GAATGC 2 cut(s) 520, 811
BsnI GGCC 3 cut(s) 273, 572, 758
Bso31I GGTCTC 1 cut(s) 198
BsoBI CYCGRG 1 cut(s) 138
Bsp119I TTCGAA 1 cut(s) 110
Bsp1286I GDGCHC 1 cut(s) 628
Bsp13I TCCGGA 1 cut(s) 83
Bsp143I GATC 2 cut(s) 153, 454
BspACI CCGC 2 cut(s) 37, 495
BspANI GGCC 3 cut(s) 273, 572, 758
BspCNI CTCAG 1 cut(s) 1132
BspEI TCCGGA 1 cut(s) 83
BspHI TCATGA 2 cut(s) 346, 565
BspLI GGNNCC 6 cut(s) 66, 97, 155, 391, 856, 1223
BspMAI CTGCAG 2 cut(s) 666, 1189
BspPI GGATC 2 cut(s) 148, 161
BspT104I TTCGAA 1 cut(s) 110
BspTNI GGTCTC 1 cut(s) 198
BsrFI RCCGGY 1 cut(s) 67
BsrI ACTGG 3 cut(s) 129, 661, 846
BssAI RCCGGY 1 cut(s) 67
BssMI GATC 2 cut(s) 153, 454
BssNAI GTATAC 1 cut(s) 337
Bst1107I GTATAC 1 cut(s) 337
Bst2UI CCWGG 2 cut(s) 62, 630
Bst4CI ACNGT 2 cut(s) 1150, 1243
BstAPI GCANNNNNTGC 2 cut(s) 609, 1208
BstBI TTCGAA 1 cut(s) 110
BstDEI CTNAG 3 cut(s) 169, 816, 1119
BstENI CCTNNNNNAGG 1 cut(s) 618
BstF5I GGATG 4 cut(s) 638, 826, 1141, 1304
BstKTI GATC 2 cut(s) 156, 457
BstMAI GTCTC 3 cut(s) 198, 886, 962
BstMBI GATC 2 cut(s) 153, 454
BstMWI GCNNNNNNNGC 6 cut(s) 73, 485, 609, 684, 1163, 1208
BstNI CCWGG 2 cut(s) 62, 630
BstSCI CCNGG 3 cut(s) 60, 628, 1218
BstSFI CTRYAG 3 cut(s) 662, 924, 1185
BstSLI GKGCMC 1 cut(s) 628
BstV1I GCAGC 4 cut(s) 796, 949, 1171, 1195
BstV2I GAAGAC 1 cut(s) 1095
BstX2I RGATCY 2 cut(s) 153, 454
BstYI RGATCY 2 cut(s) 153, 454
BstZ17I GTATAC 1 cut(s) 337
BsuRI GGCC 3 cut(s) 273, 572, 758
BtrI CACGTC 1 cut(s) 55
BtsCI GGATG 4 cut(s) 638, 826, 1141, 1304
CaiI CAGNNNCTG 1 cut(s) 1154
CciI TCATGA 2 cut(s) 346, 565
Cfr10I RCCGGY 1 cut(s) 67
Cfr13I GGNCC 4 cut(s) 58, 70, 854, 1222
Csp6I GTAC 1 cut(s) 149
CspCI CAANNNNNGTGG 2 cut(s) 382, 417
CviAII CATG 3 cut(s) 347, 544, 566
CviQI GTAC 1 cut(s) 149
DdeI CTNAG 3 cut(s) 169, 816, 1119
DpnI GATC 2 cut(s) 155, 456
DpnII GATC 2 cut(s) 153, 454
DrdI GACNNNNNNGTC 1 cut(s) 140
DriI GACNNNNNGTC 1 cut(s) 539
DseDI GACNNNNNNGTC 1 cut(s) 140
EaeI YGGCCR 1 cut(s) 756
Eam1105I GACNNNNNGTC 1 cut(s) 539
Eco147I AGGCCT 1 cut(s) 572
Eco31I GGTCTC 1 cut(s) 198
Eco47I GGWCC 4 cut(s) 58, 70, 854, 1222
Eco88I CYCGRG 1 cut(s) 138
EcoNI CCTNNNNNAGG 1 cut(s) 618
EcoO109I RGGNCCY 1 cut(s) 1222
EcoRI GAATTC 1 cut(s) 173
EcoRII CCWGG 2 cut(s) 60, 628
EcoT22I ATGCAT 2 cut(s) 320, 638
FaeI CATG 3 cut(s) 350, 547, 569
FalI AAGNNNNNCTT 2 cut(s) 856, 888
FaqI GGGAC 4 cut(s) 239, 286, 840, 1235
FatI CATG 3 cut(s) 346, 543, 565
FblI GTMKAC 1 cut(s) 336
Fnu4HI GCNGC 4 cut(s) 785, 963, 1185, 1209
FokI GGATG 4 cut(s) 645, 833, 1148, 1291
Fsp4HI GCNGC 4 cut(s) 785, 963, 1185, 1209
FspBI CTAG 2 cut(s) 293, 539
GluI GCNGC 4 cut(s) 785, 963, 1185, 1209
GsuI CTGGAG 1 cut(s) 83
HaeIII GGCC 3 cut(s) 273, 572, 758
HapII CCGG 5 cut(s) 40, 68, 84, 788, 1219
Hin1II CATG 3 cut(s) 350, 547, 569
HincII GTYRAC 1 cut(s) 1288
HindII GTYRAC 1 cut(s) 1288
HindIII AAGCTT 1 cut(s) 685
HinfI GANTC 5 cut(s) 87, 644, 845, 970, 1160
HpaII CCGG 5 cut(s) 40, 68, 84, 788, 1219
Hpy166II GTNNAC 6 cut(s) 58, 337, 533, 626, 708, 1288
Hpy188I TCNGA 5 cut(s) 49, 92, 422, 1099, 1318
Hpy188III TCNNGA 9 cut(s) 84, 178, 293, 347, 566, 764, 954, 1258, 1308
Hpy8I GTNNAC 6 cut(s) 58, 337, 533, 626, 708, 1288
Hpy99I CGWCG 3 cut(s) 29, 53, 56
HpyAV CCTTC 4 cut(s) 379, 668, 1066, 1276
HpyCH4III ACNGT 2 cut(s) 1150, 1243
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 9 cut(s) 281, 318, 603, 626, 636, 664, 715, 1187, 1211
HpyF10VI GCNNNNNNNGC 6 cut(s) 73, 485, 609, 684, 1163, 1208
HpyF3I CTNAG 3 cut(s) 169, 816, 1119
HpySE526I ACGT 1 cut(s) 54
Hsp92II CATG 3 cut(s) 350, 547, 569
Kpn2I TCCGGA 1 cut(s) 83
Kzo9I GATC 2 cut(s) 153, 454
LmnI GCTCC 1 cut(s) 64
Lsp1109I GCAGC 4 cut(s) 796, 949, 1171, 1195
LweI GCATC 4 cut(s) 612, 623, 645, 1085
MaeI CTAG 2 cut(s) 293, 539
MaeII ACGT 1 cut(s) 54
MaeIII GTNAC 2 cut(s) 555, 1232
MalI GATC 2 cut(s) 155, 456
MboI GATC 2 cut(s) 153, 454
MboII GAAGA 3 cut(s) 193, 464, 1100
MfeI CAATTG 2 cut(s) 282, 1083
MflI RGATCY 2 cut(s) 153, 454
MhlI GDGCHC 1 cut(s) 628
MlsI TGGCCA 1 cut(s) 758
MluCI AATT 9 cut(s) 173, 246, 282, 435, 467, 513, 772, 792, 1083
MluNI TGGCCA 1 cut(s) 758
MlyI GAGTC 1 cut(s) 964
MmeI TCCRAC 2 cut(s) 72, 445
MnlI CCTC 7 cut(s) 86, 284, 325, 562, 798, 1128, 1323
Mox20I TGGCCA 1 cut(s) 758
Mph1103I ATGCAT 2 cut(s) 320, 638
MroI TCCGGA 1 cut(s) 83
MscI TGGCCA 1 cut(s) 758
MseI TTAA 6 cut(s) 446, 459, 653, 771, 939, 1229
MslI CAYNNNNRTG 1 cut(s) 631
Msp20I TGGCCA 1 cut(s) 758
MspA1I CMGCKG 1 cut(s) 721
MspI CCGG 5 cut(s) 40, 68, 84, 788, 1219
MspR9I CCNGG 3 cut(s) 62, 630, 1220
MunI CAATTG 2 cut(s) 282, 1083
Mva1269I GAATGC 2 cut(s) 520, 811
MvaI CCWGG 2 cut(s) 62, 630
MwoI GCNNNNNNNGC 6 cut(s) 73, 485, 609, 684, 1163, 1208
NciI CCSGG 1 cut(s) 1220
NdeII GATC 2 cut(s) 153, 454
NlaIII CATG 3 cut(s) 350, 547, 569
NlaIV GGNNCC 6 cut(s) 66, 97, 155, 391, 856, 1223
NmeAIII GCCGAG 1 cut(s) 991
NmuCI GTSAC 2 cut(s) 555, 1232
NsiI ATGCAT 2 cut(s) 320, 638
NspV TTCGAA 1 cut(s) 110
PagI TCATGA 2 cut(s) 346, 565
PceI AGGCCT 1 cut(s) 572
PctI GAATGC 2 cut(s) 520, 811
PfeI GAWTC 4 cut(s) 87, 644, 845, 1160
PflMI CCANNNNNTGG 1 cut(s) 724
PkrI GCNGC 4 cut(s) 786, 964, 1186, 1210
PleI GAGTC 1 cut(s) 964
PpsI GAGTC 1 cut(s) 964
PpuMI RGGWCCY 1 cut(s) 1222
Psp5II RGGWCCY 1 cut(s) 1222
Psp6I CCWGG 2 cut(s) 60, 628
PspGI CCWGG 2 cut(s) 60, 628
PspN4I GGNNCC 6 cut(s) 66, 97, 155, 391, 856, 1223
PspPI GGNCC 4 cut(s) 58, 70, 854, 1222
PspPPI RGGWCCY 1 cut(s) 1222
PstI CTGCAG 2 cut(s) 666, 1189
PstNI CAGNNNCTG 1 cut(s) 1154
PsuI RGATCY 2 cut(s) 153, 454
PvuII CAGCTG 1 cut(s) 721
RsaI GTAC 1 cut(s) 150
RsaNI GTAC 1 cut(s) 149
RseI CAYNNNNRTG 1 cut(s) 631
SaqAI TTAA 6 cut(s) 446, 459, 653, 771, 939, 1229
SatI GCNGC 4 cut(s) 785, 963, 1185, 1209
Sau3AI GATC 2 cut(s) 153, 454
Sau96I GGNCC 4 cut(s) 58, 70, 854, 1222
SchI GAGTC 1 cut(s) 964
ScrFI CCNGG 3 cut(s) 62, 630, 1220
SduI GDGCHC 1 cut(s) 628
SfaNI GCATC 4 cut(s) 612, 623, 645, 1085
SfcI CTRYAG 3 cut(s) 662, 924, 1185
SfuI TTCGAA 1 cut(s) 110
SinI GGWCC 4 cut(s) 58, 70, 854, 1222
SmiMI CAYNNNNRTG 1 cut(s) 631
Sse9I AATT 9 cut(s) 173, 246, 282, 435, 467, 513, 772, 792, 1083
SseBI AGGCCT 1 cut(s) 572
SsiI CCGC 2 cut(s) 37, 495
SspMI CTAG 2 cut(s) 293, 539
StuI AGGCCT 1 cut(s) 572
StyD4I CCNGG 3 cut(s) 60, 628, 1218
TaaI ACNGT 2 cut(s) 1150, 1243
TaiI ACGT 1 cut(s) 57
TaqI TCGA 4 cut(s) 24, 110, 132, 1070
TasI AATT 9 cut(s) 173, 246, 282, 435, 467, 513, 772, 792, 1083
TfiI GAWTC 4 cut(s) 87, 644, 845, 1160
Tru1I TTAA 6 cut(s) 446, 459, 653, 771, 939, 1229
Tru9I TTAA 6 cut(s) 446, 459, 653, 771, 939, 1229
TseFI GTSAC 2 cut(s) 555, 1232
TseI GCWGC 4 cut(s) 784, 962, 1184, 1208
Tsp45I GTSAC 2 cut(s) 555, 1232
TspDTI ATGAA 4 cut(s) 258, 335, 551, 1158
TspGWI ACGGA 1 cut(s) 166
Van91I CCANNNNNTGG 1 cut(s) 724
VneI GTGCAC 1 cut(s) 624
VpaK11BI GGWCC 4 cut(s) 58, 70, 854, 1222
XagI CCTNNNNNAGG 1 cut(s) 618
XapI RAATTY 2 cut(s) 173, 792
XbaI TCTAGA 1 cut(s) 292
XcmI CCANNNNNNNNNTGG 1 cut(s) 771
XmiI GTMKAC 1 cut(s) 336
XspI CTAG 2 cut(s) 293, 539
Zsp2I ATGCAT 2 cut(s) 320, 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.