FvH4_6g00170

Phospholipase A(1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005852
Physical Location & Seq
Reverse (-)
139582 .. 144527
4946 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g00170.t8

Sequence Viewer

Length: 1326 bp
ATGCTGTGCTTCTGCCCCTGTTTCGATGAGGAGCTGGACCTGGAGCTGGAGCCGGTGCTGCTCATCTCCGGAATCGGAGGCTCCATTCTAACTTGTAAGAAGAAGACCAAGTTGGGGTTCGACACTCGCGTCTGGGTACGCATCCTTTTGGCCGACCTTGAATTCAAGAAGAAACTCTGGTCTCTCTATAATCCCCTTACAGGTTATACAGAAACATTAGACAAGGACACTGAAATCGTGGTCCCAGATGATGATTATGGCCTTTATGCAATTGATATTCTGGACCCGTCAGCGTTTGTGAAATGCATACATTTGCAAGACGTATACTATTTTCATGATATGATTAACATGCTTTTGGGTTGCGGCTATAAGAAGGGAACCACATTGTTTGGATATGGCTATGATTTTCGACAAAGCAACAGAATTGACGAGTTAATGGAAGATCTTAAAGTGAAATTGGAAACTGCTTACAAGGCTTCTGGGGGTAGAAAAGTTAATATAATTTCGCATTCAATGGGTGGACTGCTAGTCATGTGTTTTATGTCACTCCATCATGAGGTCTTTTCGAAGTATGTGAGTAAATGGATTTGCATCGGTTGCCCTTTTCAAGGTGCACCAGGATGCATCAATGATTCTCTTTTAACCGGACTGCAGTTTGTTGAAGGCTTGGAAAGCTTCTTTTTTGTATCAAGGTGGACTATGCACCAGCTGTTGGTCGAGTGTCCATCAATCTACGAGATGTTGGCCAATCCAGATTTTAATTGGAAAAAGGAGCCGGAAATTCAGGTTTGGAGGAAGCAGTCTAAGAATGGGAAAAGTTCTATTGAGCTGGAATCTTACGGCCCCGCTGAAAGTAACACTTTGTTTAGAGAAGCATTGAGAAATAATGAGCTAACTTATGATGGTAAAACTGTAGCTTTGCCATTTAACTTTGCTATCTCCAGATGGGCTGCCGAGACTCGTCAAGTTCTCAACAATGCCAAACTACCAGATGGAGTCTGCTTCTATAACATTTATGGGATATCCTTTGACACACCATTTGATGTTTGCTATGGCTCGAAGGAATCTCCAATTGAAGACTTGTCTGAAATTTGCCATTCAATGCCTCAGTATTCTTATGTGGATGGAGATGAAACAGTTTCTGCTGAATCAGCAAAGGCAGATGGATTTGATGCAGTTGAAAGAGTGGCAATAGCTGCGAGACACCGGGAACTATTAAGTGACAAAACGGTTTTTAAACATATCAAAAAGTGGTTGGGAGTTGAGCAGAAGGGCAACATACATTCCAAGACATCCAGAGTTTCAGATGAGCCTGCGAGGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko00592 ko01100 ko01110 map00564 map00592 map01100 map01110
Pfam Domains
Protein Families
LCAT

Protein Analysis

442

Amino Acids

50.1

Weight (kDa)

5.35

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 83 - 388 1.5e-24 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 128
AccB7I CCANNNNNTGG 1 cut(s) 712
AccI GTMKAC 1 cut(s) 324
AccII CGCG 1 cut(s) 129
AccIII TCCGGA 1 cut(s) 68
AciI CCGC 2 cut(s) 363, 846
AcoI YGGCCR 2 cut(s) 150, 744
AcsI RAATTY 3 cut(s) 161, 780, 1089
AfaI GTAC 1 cut(s) 138
AfiI CCNNNNNNNGG 6 cut(s) 46, 114, 200, 556, 608, 712
AgsI TTSAA 8 cut(s) 161, 166, 513, 608, 662, 1076, 1101, 1181
AhdI GACNNNNNGTC 1 cut(s) 527
AjnI CCWGG 2 cut(s) 39, 616
AjuI GAANNNNNNNTTGG 4 cut(s) 95, 101, 127, 133
AluBI AGCT 8 cut(s) 34, 46, 675, 709, 829, 892, 917, 1196
AluI AGCT 8 cut(s) 34, 46, 675, 709, 829, 892, 917, 1196
Alw21I GWGCWC 1 cut(s) 616
Alw26I GTCTC 3 cut(s) 186, 950, 1195
Alw44I GTGCAC 1 cut(s) 612
AlwNI CAGNNNCTG 1 cut(s) 1142
Aor13HI TCCGGA 1 cut(s) 68
AoxI GGCC 4 cut(s) 150, 259, 744, 841
ApaLI GTGCAC 1 cut(s) 612
ApeKI GCWGC 3 cut(s) 58, 950, 1196
ApoI RAATTY 3 cut(s) 161, 780, 1089
ArsI GACNNNNNNTTYG 2 cut(s) 1022, 1054
AspS9I GGNCC 4 cut(s) 37, 241, 283, 842
AsuC2I CCSGG 1 cut(s) 1208
AsuII TTCGAA 1 cut(s) 566
AvaII GGWCC 3 cut(s) 37, 241, 283
BaeGI GKGCMC 1 cut(s) 616
BalI TGGCCA 1 cut(s) 746
BbsI GAAGAC 2 cut(s) 110, 1083
Bbv12I GWGCWC 1 cut(s) 616
BbvI GCAGC 3 cut(s) 45, 937, 1183
BccI CCATC 7 cut(s) 558, 733, 896, 939, 986, 1118, 1157
BceAI ACGGC 1 cut(s) 856
BciT130I CCWGG 2 cut(s) 41, 618
BcnI CCSGG 1 cut(s) 1208
BcoDI GTCTC 3 cut(s) 186, 950, 1195
BfaI CTAG 1 cut(s) 527
BfmI CTRYAG 2 cut(s) 650, 912
BglI GCCNNNNNGGC 1 cut(s) 1318
BglII AGATCT 1 cut(s) 442
BisI GCNGC 4 cut(s) 59, 364, 951, 1197
BlsI GCNGC 4 cut(s) 60, 365, 952, 1198
Bme1390I CCNGG 3 cut(s) 41, 618, 1208
Bme18I GGWCC 3 cut(s) 37, 241, 283
BmeRI GACNNNNNGTC 1 cut(s) 527
BmgT120I GGNCC 4 cut(s) 37, 241, 283, 842
BmiI GGNNCC 7 cut(s) 51, 82, 243, 285, 379, 774, 844
BmrFI CCNGG 3 cut(s) 41, 618, 1208
BmsI GCATC 5 cut(s) 150, 600, 611, 633, 1162
BpiI GAAGAC 2 cut(s) 110, 1083
BpmI CTGGAG 3 cut(s) 62, 68, 925
Bpu14I TTCGAA 1 cut(s) 566
BpuMI CCSGG 1 cut(s) 1208
BsaBI GATNNNNATC 1 cut(s) 590
BsaI GGTCTC 1 cut(s) 186
BsaWI WCCGGW 2 cut(s) 68, 644
Bsc4I CCNNNNNNNGG 6 cut(s) 46, 114, 200, 556, 608, 712
Bse118I RCCGGY 1 cut(s) 52
Bse8I GATNNNNATC 1 cut(s) 590
BseAI TCCGGA 1 cut(s) 68
BseBI CCWGG 2 cut(s) 41, 618
BseGI GGATG 4 cut(s) 141, 626, 1129, 1292
BseJI GATNNNNATC 1 cut(s) 590
BseLI CCNNNNNNNGG 6 cut(s) 46, 114, 200, 556, 608, 712
BseMII CTCAG 1 cut(s) 1121
BseRI GAGGAG 1 cut(s) 44
BseSI GKGCMC 1 cut(s) 616
BseXI GCAGC 3 cut(s) 45, 937, 1183
Bsh1236I CGCG 1 cut(s) 129
BshFI GGCC 4 cut(s) 152, 261, 746, 843
BsiHKAI GWGCWC 1 cut(s) 616
BsiSI CCGG 5 cut(s) 53, 69, 645, 776, 1207
BslFI GGGAC 1 cut(s) 227
BslI CCNNNNNNNGG 6 cut(s) 46, 114, 200, 556, 608, 712
BsmAI GTCTC 3 cut(s) 186, 950, 1195
BsmFI GGGAC 1 cut(s) 227
BsmI GAATGC 1 cut(s) 508
BsnI GGCC 4 cut(s) 152, 261, 746, 843
Bso31I GGTCTC 1 cut(s) 186
Bsp119I TTCGAA 1 cut(s) 566
Bsp1286I GDGCHC 1 cut(s) 616
Bsp13I TCCGGA 1 cut(s) 68
Bsp143I GATC 1 cut(s) 442
BspACI CCGC 2 cut(s) 363, 846
BspANI GGCC 4 cut(s) 152, 261, 746, 843
BspCNI CTCAG 1 cut(s) 1120
BspEI TCCGGA 1 cut(s) 68
BspFNI CGCG 1 cut(s) 129
BspHI TCATGA 2 cut(s) 334, 553
BspLI GGNNCC 7 cut(s) 51, 82, 243, 285, 379, 774, 844
BspMAI CTGCAG 1 cut(s) 654
BspT104I TTCGAA 1 cut(s) 566
BspTNI GGTCTC 1 cut(s) 186
BsrFI RCCGGY 1 cut(s) 52
BssAI RCCGGY 1 cut(s) 52
BssMI GATC 1 cut(s) 442
BssNAI GTATAC 1 cut(s) 325
Bst1107I GTATAC 1 cut(s) 325
Bst2UI CCWGG 2 cut(s) 41, 618
Bst4CI ACNGT 3 cut(s) 913, 1138, 1231
BstAPI GCANNNNNTGC 2 cut(s) 597, 1196
BstBI TTCGAA 1 cut(s) 566
BstC8I GCNNGC 1 cut(s) 1314
BstDEI CTNAG 2 cut(s) 804, 1107
BstENI CCTNNNNNAGG 1 cut(s) 606
BstF5I GGATG 4 cut(s) 141, 626, 1129, 1292
BstFNI CGCG 1 cut(s) 129
BstKTI GATC 1 cut(s) 445
BstMAI GTCTC 3 cut(s) 186, 950, 1195
BstMBI GATC 1 cut(s) 442
BstMWI GCNNNNNNNGC 7 cut(s) 58, 473, 597, 672, 1151, 1196, 1318
BstNI CCWGG 2 cut(s) 41, 618
BstNSI RCATGY 1 cut(s) 352
BstSCI CCNGG 3 cut(s) 39, 616, 1206
BstSFI CTRYAG 2 cut(s) 650, 912
BstSLI GKGCMC 1 cut(s) 616
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 3 cut(s) 45, 937, 1183
BstV2I GAAGAC 2 cut(s) 110, 1083
BstX2I RGATCY 1 cut(s) 442
BstYI RGATCY 1 cut(s) 442
BstZ17I GTATAC 1 cut(s) 325
BsuRI GGCC 4 cut(s) 152, 261, 746, 843
BtsCI GGATG 4 cut(s) 141, 626, 1129, 1292
BtsIMutI CAGTG 1 cut(s) 228
Cac8I GCNNGC 1 cut(s) 1314
CaiI CAGNNNCTG 1 cut(s) 1142
CciI TCATGA 2 cut(s) 334, 553
Cfr10I RCCGGY 1 cut(s) 52
Cfr13I GGNCC 4 cut(s) 37, 241, 283, 842
CseI GACGC 1 cut(s) 118
Csp6I GTAC 1 cut(s) 137
CspCI CAANNNNNGTGG 2 cut(s) 370, 405
CviAII CATG 4 cut(s) 335, 349, 532, 554
CviQI GTAC 1 cut(s) 137
DdeI CTNAG 2 cut(s) 804, 1107
DpnI GATC 1 cut(s) 444
DpnII GATC 1 cut(s) 442
DraI TTTAAA 1 cut(s) 1237
DrdI GACNNNNNNGTC 1 cut(s) 128
DriI GACNNNNNGTC 1 cut(s) 527
DseDI GACNNNNNNGTC 1 cut(s) 128
EaeI YGGCCR 2 cut(s) 150, 744
Eam1105I GACNNNNNGTC 1 cut(s) 527
Eco31I GGTCTC 1 cut(s) 186
Eco32I GATATC 1 cut(s) 1023
Eco47I GGWCC 3 cut(s) 37, 241, 283
EcoNI CCTNNNNNAGG 1 cut(s) 606
EcoRI GAATTC 1 cut(s) 161
EcoRII CCWGG 2 cut(s) 39, 616
EcoRV GATATC 1 cut(s) 1023
EcoT22I ATGCAT 2 cut(s) 308, 626
FaeI CATG 4 cut(s) 338, 352, 535, 557
FalI AAGNNNNNCTT 2 cut(s) 844, 876
FaqI GGGAC 1 cut(s) 227
FatI CATG 4 cut(s) 334, 348, 531, 553
FauI CCCGC 1 cut(s) 853
FblI GTMKAC 1 cut(s) 324
Fnu4HI GCNGC 4 cut(s) 59, 364, 951, 1197
FokI GGATG 4 cut(s) 128, 633, 1136, 1279
Fsp4HI GCNGC 4 cut(s) 59, 364, 951, 1197
FspBI CTAG 1 cut(s) 527
GluI GCNGC 4 cut(s) 59, 364, 951, 1197
GsuI CTGGAG 3 cut(s) 62, 68, 925
HaeIII GGCC 4 cut(s) 152, 261, 746, 843
HapII CCGG 5 cut(s) 53, 69, 645, 776, 1207
HgaI GACGC 1 cut(s) 118
Hin1II CATG 4 cut(s) 338, 352, 535, 557
HindIII AAGCTT 1 cut(s) 673
HinfI GANTC 7 cut(s) 72, 632, 833, 958, 996, 1064, 1148
HpaII CCGG 5 cut(s) 53, 69, 645, 776, 1207
Hpy166II GTNNAC 4 cut(s) 325, 521, 614, 696
Hpy188I TCNGA 3 cut(s) 77, 1087, 1306
Hpy188III TCNNGA 8 cut(s) 69, 166, 281, 335, 554, 752, 942, 1296
Hpy8I GTNNAC 4 cut(s) 325, 521, 614, 696
HpyAV CCTTC 4 cut(s) 367, 656, 1054, 1264
HpyCH4III ACNGT 3 cut(s) 913, 1138, 1231
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 9 cut(s) 269, 306, 316, 591, 614, 624, 652, 703, 1175
HpyF10VI GCNNNNNNNGC 7 cut(s) 58, 473, 597, 672, 1151, 1196, 1318
HpyF3I CTNAG 2 cut(s) 804, 1107
HpySE526I ACGT 1 cut(s) 321
Hsp92II CATG 4 cut(s) 338, 352, 535, 557
Kpn2I TCCGGA 1 cut(s) 68
Kzo9I GATC 1 cut(s) 442
LmnI GCTCC 5 cut(s) 31, 43, 49, 86, 772
Lsp1109I GCAGC 3 cut(s) 45, 937, 1183
LweI GCATC 5 cut(s) 150, 600, 611, 633, 1162
MaeI CTAG 1 cut(s) 527
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 3 cut(s) 543, 854, 1220
MalI GATC 1 cut(s) 444
MboI GATC 1 cut(s) 442
MboII GAAGA 5 cut(s) 112, 115, 181, 452, 1088
MfeI CAATTG 2 cut(s) 270, 1071
MflI RGATCY 1 cut(s) 442
MhlI GDGCHC 1 cut(s) 616
MlsI TGGCCA 1 cut(s) 746
MluCI AATT 9 cut(s) 161, 270, 423, 455, 501, 760, 780, 1071, 1089
MluNI TGGCCA 1 cut(s) 746
MlyI GAGTC 2 cut(s) 952, 1005
MnlI CCTC 6 cut(s) 22, 71, 550, 786, 1116, 1311
Mox20I TGGCCA 1 cut(s) 746
Mph1103I ATGCAT 2 cut(s) 308, 626
MroI TCCGGA 1 cut(s) 68
MscI TGGCCA 1 cut(s) 746
MseI TTAA 9 cut(s) 345, 434, 447, 495, 641, 759, 927, 1217, 1236
MslI CAYNNNNRTG 1 cut(s) 619
Msp20I TGGCCA 1 cut(s) 746
MspA1I CMGCKG 2 cut(s) 709, 848
MspI CCGG 5 cut(s) 53, 69, 645, 776, 1207
MspR9I CCNGG 3 cut(s) 41, 618, 1208
MunI CAATTG 2 cut(s) 270, 1071
Mva1269I GAATGC 1 cut(s) 508
MvaI CCWGG 2 cut(s) 41, 618
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 7 cut(s) 58, 473, 597, 672, 1151, 1196, 1318
NciI CCSGG 1 cut(s) 1208
NdeII GATC 1 cut(s) 442
NlaIII CATG 4 cut(s) 338, 352, 535, 557
NlaIV GGNNCC 7 cut(s) 51, 82, 243, 285, 379, 774, 844
NmeAIII GCCGAG 1 cut(s) 979
NmuCI GTSAC 2 cut(s) 543, 1220
NsiI ATGCAT 2 cut(s) 308, 626
NspI RCATGY 1 cut(s) 352
NspV TTCGAA 1 cut(s) 566
PagI TCATGA 2 cut(s) 334, 553
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PctI GAATGC 1 cut(s) 508
PfeI GAWTC 5 cut(s) 72, 632, 833, 1064, 1148
PflMI CCANNNNNTGG 1 cut(s) 712
PkrI GCNGC 4 cut(s) 60, 365, 952, 1198
PleI GAGTC 2 cut(s) 952, 1004
PpsI GAGTC 2 cut(s) 952, 1004
Psp6I CCWGG 2 cut(s) 39, 616
PspGI CCWGG 2 cut(s) 39, 616
PspN4I GGNNCC 7 cut(s) 51, 82, 243, 285, 379, 774, 844
PspPI GGNCC 4 cut(s) 37, 241, 283, 842
PstI CTGCAG 1 cut(s) 654
PstNI CAGNNNCTG 1 cut(s) 1142
PsuI RGATCY 1 cut(s) 442
PvuII CAGCTG 1 cut(s) 709
RsaI GTAC 1 cut(s) 138
RsaNI GTAC 1 cut(s) 137
RseI CAYNNNNRTG 1 cut(s) 619
SaqAI TTAA 9 cut(s) 345, 434, 447, 495, 641, 759, 927, 1217, 1236
SatI GCNGC 4 cut(s) 59, 364, 951, 1197
Sau3AI GATC 1 cut(s) 442
Sau96I GGNCC 4 cut(s) 37, 241, 283, 842
SchI GAGTC 2 cut(s) 952, 1005
ScrFI CCNGG 3 cut(s) 41, 618, 1208
SduI GDGCHC 1 cut(s) 616
SfaNI GCATC 5 cut(s) 150, 600, 611, 633, 1162
SfcI CTRYAG 2 cut(s) 650, 912
SfuI TTCGAA 1 cut(s) 566
SinI GGWCC 3 cut(s) 37, 241, 283
SmiMI CAYNNNNRTG 1 cut(s) 619
Sse9I AATT 9 cut(s) 161, 270, 423, 455, 501, 760, 780, 1071, 1089
SsiI CCGC 2 cut(s) 363, 846
SspMI CTAG 1 cut(s) 527
StyD4I CCNGG 3 cut(s) 39, 616, 1206
TaaI ACNGT 3 cut(s) 913, 1138, 1231
TaiI ACGT 1 cut(s) 324
TaqI TCGA 6 cut(s) 24, 120, 409, 566, 717, 1058
TasI AATT 9 cut(s) 161, 270, 423, 455, 501, 760, 780, 1071, 1089
TauI GCSGC 1 cut(s) 366
TfiI GAWTC 5 cut(s) 72, 632, 833, 1064, 1148
Tru1I TTAA 9 cut(s) 345, 434, 447, 495, 641, 759, 927, 1217, 1236
Tru9I TTAA 9 cut(s) 345, 434, 447, 495, 641, 759, 927, 1217, 1236
TscAI CASTG 1 cut(s) 235
TseFI GTSAC 2 cut(s) 543, 1220
TseI GCWGC 3 cut(s) 58, 950, 1196
Tsp45I GTSAC 2 cut(s) 543, 1220
TspDTI ATGAA 2 cut(s) 323, 1146
TspRI CASTG 1 cut(s) 235
Van91I CCANNNNNTGG 1 cut(s) 712
VneI GTGCAC 1 cut(s) 612
VpaK11BI GGWCC 3 cut(s) 37, 241, 283
XagI CCTNNNNNAGG 1 cut(s) 606
XapI RAATTY 3 cut(s) 161, 780, 1089
XceI RCATGY 1 cut(s) 352
XcmI CCANNNNNNNNNTGG 1 cut(s) 759
XmiI GTMKAC 1 cut(s) 324
XspI CTAG 1 cut(s) 527
Zsp2I ATGCAT 2 cut(s) 308, 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.