MD12G1262800.v1.1

Phospholipase A(1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005852
Physical Location & Seq
Reverse (-)
32948082 .. 32952854
4773 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1262800.v1.1.491

Sequence Viewer

Length: 1365 bp
ATGCCAGGCCAGTGCTTCTGCCCCTGTTTCGACTATGAACATGACAACCGCACTTCCTCTCAAACGGAGGTGGAGGTGGAGGTGGAGCCGGTGCTGCTGGTGTCGGGCATCGGAGGCTCCATTCTGCACTCCAAGAGGAGGAAGTTGGGCTTCGACACACGGGTCTGGGTCCGGATCTTATTGGCTGACTTGGAGTTCAAGAAGAAGCTCTGGTCTATCTACAATCCCCAAACAGGTTATACAGAAACGCTAGACAAAAAAACTGAAATTGTGGTCCCAGATGACGATTACGGCCTGTATGCCATTGATATTCTAGATCCTTGCTGGTTTGTCAAATTGATACGTTTGAAAGAGGTATACCAGTTCCATGATATGATTGATATGCTTGTCGAGTGTGGGTATAAAAAGGGAACCACTTTGTTTGGATACGGTTATGATTTTCGACAAAGCAATAGAATTGACAAGTCAATGGAGGGTCTTAAGGTGAAACTGGAAACTGCTTACAAGGCTTCTGGGGGTAGAAAAGTGAATATAATTTCGCATTCAGTGGGTGGACTGCTAGTCACATGCTTTATGTCACTCCATACTGATGTATTTTCAAAGTACGTGAGTAAATGGATCTGCATAGCTTGCCCTTTTCAAGGTGCACCAGGATGCATCAATGATTCTCTCTTAACTGGATTGCAGTTTGTTGAAGGCTTGGGAAGTTACCTTTTTGTATCAAGGTGGACCATGCACCAGCTGTTGATTGAGTGTCCATCAATCTATGAGATGTTGGCAAATCCAAAATTTAATTGGAAAAGGCCGCCAGAAATTCAGGTTTGGAGGAACAAATCCACGGATGGGGAAACTTTGGTTGATTTGGAGTCTTATGGCCCAATTGAAAGCATCCCTTTGTTTGAAGAAGCATTGAAATATAATGAGCTAAATTATGATGGTGAAACGGTAGCTTTGCCATTTAACCATTCTATTCTCAGATGGGCTGCTGAGACTCGCCAAGTTTTGAACAGTGCTAAACTACCAGATAGCGTCAGCTTCTATAACATATATGGCACATCGTTCGGCACCCCTTTTGATGTTTGCTACGGTTCGGAGACAGCTCCAATTGAGGACCTGTCTGAAATATGCCATTCAATACCTCGGTATTCTTATGTGGATGGAGATGAAACAGTTCCGGCTGAGTCAGCAAAGGCGGATGGAATTGCTGCAGTGGAAAGAGTAGCAATAGCTGCAAGGCACCGGGAACTATTACGGGACAAAACAGTTTTTCAACATATCCTAAGGTGGTTGGGAGTCGAACAGAAGGTCAGCAGACATTCTAAGACATCCAGAGTTGCAGACGCCTCTTCAAATAAGCGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko00592 ko01100 ko01110 map00564 map00592 map01100 map01110
Pfam Domains
Protein Families
LCAT

Protein Analysis

455

Amino Acids

51.5

Weight (kDa)

5.84

Isoelectric Point (pI)

47.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 73 - 396 3.9e-22 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 161
AccB1I GGYRCC 2 cut(s) 1064, 1236
AccI GTMKAC 1 cut(s) 357
AccIII TCCGGA 1 cut(s) 171
AciI CCGC 3 cut(s) 49, 806, 1193
AclWI GGATC 3 cut(s) 182, 311, 626
AcsI RAATTY 2 cut(s) 788, 813
AcyI GRCGYC 1 cut(s) 1341
AfaI GTAC 1 cut(s) 605
AfiI CCNNNNNNNGG 4 cut(s) 138, 233, 641, 843
AflII CTTAAG 1 cut(s) 479
AhdI GACNNNNNGTC 1 cut(s) 560
AjnI CCWGG 2 cut(s) 4, 649
AluBI AGCT 8 cut(s) 208, 629, 742, 925, 950, 1035, 1100, 1229
AluI AGCT 8 cut(s) 208, 629, 742, 925, 950, 1035, 1100, 1229
Alw21I GWGCWC 1 cut(s) 649
Alw26I GTCTC 2 cut(s) 983, 1088
Alw44I GTGCAC 1 cut(s) 645
AlwI GGATC 3 cut(s) 182, 311, 626
Aor13HI TCCGGA 1 cut(s) 171
AoxI GGCC 4 cut(s) 7, 292, 803, 874
ApaLI GTGCAC 1 cut(s) 645
ApeKI GCWGC 4 cut(s) 94, 983, 1205, 1229
ApoI RAATTY 2 cut(s) 788, 813
ArsI GACNNNNNNTTYG 2 cut(s) 1290, 1322
Asp700I GAANNNNTTC 1 cut(s) 1170
AspS9I GGNCC 5 cut(s) 169, 274, 729, 875, 1111
AsuC2I CCSGG 1 cut(s) 1241
AsuHPI GGTGA 2 cut(s) 496, 950
AvaII GGWCC 4 cut(s) 169, 274, 729, 1111
AxyI CCTNAGG 1 cut(s) 1280
BaeGI GKGCMC 1 cut(s) 649
BanI GGYRCC 2 cut(s) 1064, 1236
Bbv12I GWGCWC 1 cut(s) 649
BbvI GCAGC 4 cut(s) 81, 970, 1192, 1216
BccI CCATC 6 cut(s) 766, 836, 929, 972, 1151, 1190
BceAI ACGGC 1 cut(s) 307
BcgI CGANNNNNNTGC 2 cut(s) 1042, 1076
BciT130I CCWGG 2 cut(s) 6, 651
BciVI GTATCC 1 cut(s) 419
BcnI CCSGG 1 cut(s) 1241
BcoDI GTCTC 2 cut(s) 983, 1088
BfaI CTAG 3 cut(s) 251, 314, 560
BfmI CTRYAG 1 cut(s) 1206
BfrI CTTAAG 1 cut(s) 479
BfuI GTATCC 1 cut(s) 419
BisI GCNGC 5 cut(s) 95, 806, 984, 1206, 1230
BlsI GCNGC 5 cut(s) 96, 807, 985, 1207, 1231
Bme1390I CCNGG 3 cut(s) 6, 651, 1241
Bme18I GGWCC 4 cut(s) 169, 274, 729, 1111
BmeRI GACNNNNNGTC 1 cut(s) 560
BmgT120I GGNCC 5 cut(s) 169, 274, 729, 875, 1111
BmiI GGNNCC 7 cut(s) 87, 118, 170, 276, 412, 1066, 1238
BmrFI CCNGG 3 cut(s) 6, 651, 1241
BmsI GCATC 4 cut(s) 117, 644, 666, 897
BpuMI CCSGG 1 cut(s) 1241
BsaAI YACGTR 1 cut(s) 607
BsaHI GRCGYC 1 cut(s) 1341
BsaJI CCNNGG 2 cut(s) 837, 1139
BsaWI WCCGGW 1 cut(s) 171
Bsc4I CCNNNNNNNGG 4 cut(s) 138, 233, 641, 843
Bse118I RCCGGY 1 cut(s) 88
Bse1I ACTGG 4 cut(s) 10, 361, 495, 682
Bse21I CCTNAGG 1 cut(s) 1280
BseAI TCCGGA 1 cut(s) 171
BseBI CCWGG 2 cut(s) 6, 651
BseDI CCNNGG 2 cut(s) 837, 1139
BseGI GGATG 6 cut(s) 659, 847, 888, 1162, 1201, 1325
BseLI CCNNNNNNNGG 4 cut(s) 138, 233, 641, 843
BseMII CTCAG 3 cut(s) 978, 988, 1170
BseNI ACTGG 4 cut(s) 10, 361, 495, 682
BseRI GAGGAG 1 cut(s) 151
BseSI GKGCMC 1 cut(s) 649
BseXI GCAGC 4 cut(s) 81, 970, 1192, 1216
BsgI GTGCAG 1 cut(s) 110
BshFI GGCC 4 cut(s) 9, 294, 805, 876
BshNI GGYRCC 2 cut(s) 1064, 1236
BsiHKAI GWGCWC 1 cut(s) 649
BsiSI CCGG 4 cut(s) 89, 172, 1175, 1240
BslFI GGGAC 2 cut(s) 260, 1268
BslI CCNNNNNNNGG 4 cut(s) 138, 233, 641, 843
BsmAI GTCTC 2 cut(s) 983, 1088
BsmFI GGGAC 2 cut(s) 260, 1268
BsmI GAATGC 1 cut(s) 541
BsnI GGCC 4 cut(s) 9, 294, 805, 876
Bsp1286I GDGCHC 1 cut(s) 649
Bsp13I TCCGGA 1 cut(s) 171
Bsp143I GATC 3 cut(s) 174, 316, 618
BspACI CCGC 3 cut(s) 49, 806, 1193
BspANI GGCC 4 cut(s) 9, 294, 805, 876
BspCNI CTCAG 3 cut(s) 979, 987, 1171
BspEI TCCGGA 1 cut(s) 171
BspLI GGNNCC 7 cut(s) 87, 118, 170, 276, 412, 1066, 1238
BspMAI CTGCAG 1 cut(s) 1210
BspPI GGATC 3 cut(s) 182, 311, 626
BspT107I GGYRCC 2 cut(s) 1064, 1236
BspTI CTTAAG 1 cut(s) 479
BsrFI RCCGGY 1 cut(s) 88
BsrI ACTGG 4 cut(s) 10, 361, 495, 682
BssAI RCCGGY 1 cut(s) 88
BssECI CCNNGG 2 cut(s) 837, 1139
BssMI GATC 3 cut(s) 174, 316, 618
BssNAI GTATAC 1 cut(s) 358
BssNI GRCGYC 1 cut(s) 1341
Bst1107I GTATAC 1 cut(s) 358
Bst2UI CCWGG 2 cut(s) 6, 651
Bst4CI ACNGT 6 cut(s) 431, 946, 1010, 1088, 1171, 1264
Bst6I CTCTTC 1 cut(s) 1351
BstACI GRCGYC 1 cut(s) 1341
BstAFI CTTAAG 1 cut(s) 479
BstAPI GCANNNNNTGC 2 cut(s) 630, 1229
BstBAI YACGTR 1 cut(s) 607
BstC8I GCNNGC 2 cut(s) 631, 1359
BstDEI CTNAG 5 cut(s) 974, 987, 1179, 1280, 1320
BstDSI CCRYGG 1 cut(s) 837
BstENI CCTNNNNNAGG 1 cut(s) 639
BstF5I GGATG 6 cut(s) 659, 847, 888, 1162, 1201, 1325
BstKTI GATC 3 cut(s) 177, 319, 621
BstMAI GTCTC 2 cut(s) 983, 1088
BstMBI GATC 3 cut(s) 174, 316, 618
BstMWI GCNNNNNNNGC 6 cut(s) 94, 114, 506, 630, 1184, 1229
BstNI CCWGG 2 cut(s) 6, 651
BstNSI RCATGY 1 cut(s) 570
BstSCI CCNGG 3 cut(s) 4, 649, 1239
BstSFI CTRYAG 1 cut(s) 1206
BstSLI GKGCMC 1 cut(s) 649
BstV1I GCAGC 4 cut(s) 81, 970, 1192, 1216
BstX2I RGATCY 3 cut(s) 174, 316, 618
BstYI RGATCY 3 cut(s) 174, 316, 618
BstZ17I GTATAC 1 cut(s) 358
Bsu36I CCTNAGG 1 cut(s) 1280
BsuI GTATCC 1 cut(s) 419
BsuRI GGCC 4 cut(s) 9, 294, 805, 876
BtgI CCRYGG 1 cut(s) 837
BtsCI GGATG 6 cut(s) 659, 847, 888, 1162, 1201, 1325
BtsI GCAGTG 1 cut(s) 1215
BtsIMutI CAGTG 4 cut(s) 17, 552, 1015, 1215
Cac8I GCNNGC 2 cut(s) 631, 1359
Cfr10I RCCGGY 1 cut(s) 88
Cfr13I GGNCC 5 cut(s) 169, 274, 729, 875, 1111
CseI GACGC 2 cut(s) 1018, 1349
Csp6I GTAC 1 cut(s) 604
CspCI CAANNNNNGTGG 2 cut(s) 403, 438
CviAII CATG 4 cut(s) 41, 368, 567, 733
CviQI GTAC 1 cut(s) 604
DdeI CTNAG 5 cut(s) 974, 987, 1179, 1280, 1320
DpnI GATC 3 cut(s) 176, 318, 620
DpnII GATC 3 cut(s) 174, 316, 618
DrdI GACNNNNNNGTC 1 cut(s) 161
DriI GACNNNNNGTC 1 cut(s) 560
DseDI GACNNNNNNGTC 1 cut(s) 161
Eam1104I CTCTTC 1 cut(s) 1351
Eam1105I GACNNNNNGTC 1 cut(s) 560
EarI CTCTTC 1 cut(s) 1351
EciI GGCGGA 1 cut(s) 1208
Eco47I GGWCC 4 cut(s) 169, 274, 729, 1111
Eco81I CCTNAGG 1 cut(s) 1280
EcoNI CCTNNNNNAGG 1 cut(s) 639
EcoO109I RGGNCCY 1 cut(s) 1111
EcoRII CCWGG 2 cut(s) 4, 649
EcoT22I ATGCAT 1 cut(s) 659
FaeI CATG 4 cut(s) 44, 371, 570, 736
FalI AAGNNNNNCTT 4 cut(s) 134, 166, 877, 909
FaqI GGGAC 2 cut(s) 260, 1268
FatI CATG 4 cut(s) 40, 367, 566, 732
FblI GTMKAC 1 cut(s) 357
Fnu4HI GCNGC 5 cut(s) 95, 806, 984, 1206, 1230
FokI GGATG 6 cut(s) 666, 854, 875, 1169, 1208, 1312
Fsp4HI GCNGC 5 cut(s) 95, 806, 984, 1206, 1230
FspBI CTAG 3 cut(s) 251, 314, 560
GluI GCNGC 5 cut(s) 95, 806, 984, 1206, 1230
HaeIII GGCC 4 cut(s) 9, 294, 805, 876
HapII CCGG 4 cut(s) 89, 172, 1175, 1240
HgaI GACGC 2 cut(s) 1018, 1349
Hin1I GRCGYC 1 cut(s) 1341
Hin1II CATG 4 cut(s) 44, 371, 570, 736
HinfI GANTC 5 cut(s) 665, 866, 991, 1181, 1293
HpaII CCGG 4 cut(s) 89, 172, 1175, 1240
HphI GGTGA 2 cut(s) 496, 950
Hpy166II GTNNAC 4 cut(s) 358, 554, 647, 729
Hpy188I TCNGA 4 cut(s) 113, 977, 1093, 1120
Hpy188III TCNNGA 4 cut(s) 172, 199, 314, 1329
Hpy8I GTNNAC 4 cut(s) 358, 554, 647, 729
HpyAV CCTTC 2 cut(s) 689, 1297
HpyCH4III ACNGT 6 cut(s) 431, 946, 1010, 1088, 1171, 1264
HpyCH4IV ACGT 2 cut(s) 343, 606
HpyCH4V TGCA 9 cut(s) 127, 624, 647, 657, 685, 736, 1208, 1232, 1337
HpyF10VI GCNNNNNNNGC 6 cut(s) 94, 114, 506, 630, 1184, 1229
HpyF3I CTNAG 5 cut(s) 974, 987, 1179, 1280, 1320
HpySE526I ACGT 2 cut(s) 343, 606
Hsp92I GRCGYC 1 cut(s) 1341
Hsp92II CATG 4 cut(s) 44, 371, 570, 736
Kpn2I TCCGGA 1 cut(s) 171
Kzo9I GATC 3 cut(s) 174, 316, 618
LmnI GCTCC 3 cut(s) 85, 122, 1105
Lsp1109I GCAGC 4 cut(s) 81, 970, 1192, 1216
LweI GCATC 4 cut(s) 117, 644, 666, 897
MaeI CTAG 3 cut(s) 251, 314, 560
MaeII ACGT 2 cut(s) 343, 606
MaeIII GTNAC 3 cut(s) 562, 576, 707
MalI GATC 3 cut(s) 176, 318, 620
MboI GATC 3 cut(s) 174, 316, 618
MboII GAAGA 3 cut(s) 214, 914, 1338
MfeI CAATTG 2 cut(s) 879, 1104
MflI RGATCY 3 cut(s) 174, 316, 618
MhlI GDGCHC 1 cut(s) 649
MlyI GAGTC 4 cut(s) 875, 985, 1190, 1302
Mph1103I ATGCAT 1 cut(s) 659
MroI TCCGGA 1 cut(s) 171
MroXI GAANNNNTTC 1 cut(s) 1170
MseI TTAA 4 cut(s) 480, 674, 792, 960
MslI CAYNNNNRTG 2 cut(s) 588, 652
MspA1I CMGCKG 1 cut(s) 742
MspCI CTTAAG 1 cut(s) 479
MspI CCGG 4 cut(s) 89, 172, 1175, 1240
MspR9I CCNGG 3 cut(s) 6, 651, 1241
MunI CAATTG 2 cut(s) 879, 1104
Mva1269I GAATGC 1 cut(s) 541
MvaI CCWGG 2 cut(s) 6, 651
MwoI GCNNNNNNNGC 6 cut(s) 94, 114, 506, 630, 1184, 1229
NciI CCSGG 1 cut(s) 1241
NdeII GATC 3 cut(s) 174, 316, 618
NlaIII CATG 4 cut(s) 44, 371, 570, 736
NlaIV GGNNCC 7 cut(s) 87, 118, 170, 276, 412, 1066, 1238
NmuCI GTSAC 2 cut(s) 562, 576
NsiI ATGCAT 1 cut(s) 659
NspI RCATGY 1 cut(s) 570
PctI GAATGC 1 cut(s) 541
PdmI GAANNNNTTC 1 cut(s) 1170
PfeI GAWTC 1 cut(s) 665
PkrI GCNGC 5 cut(s) 96, 807, 985, 1207, 1231
PleI GAGTC 4 cut(s) 874, 985, 1189, 1301
PpsI GAGTC 4 cut(s) 874, 985, 1189, 1301
Ppu21I YACGTR 1 cut(s) 607
PpuMI RGGWCCY 1 cut(s) 1111
Psp5II RGGWCCY 1 cut(s) 1111
Psp6I CCWGG 2 cut(s) 4, 649
PspGI CCWGG 2 cut(s) 4, 649
PspN4I GGNNCC 7 cut(s) 87, 118, 170, 276, 412, 1066, 1238
PspPI GGNCC 5 cut(s) 169, 274, 729, 875, 1111
PspPPI RGGWCCY 1 cut(s) 1111
PstI CTGCAG 1 cut(s) 1210
PsuI RGATCY 3 cut(s) 174, 316, 618
PvuII CAGCTG 1 cut(s) 742
RsaI GTAC 1 cut(s) 605
RsaNI GTAC 1 cut(s) 604
RseI CAYNNNNRTG 2 cut(s) 588, 652
SaqAI TTAA 4 cut(s) 480, 674, 792, 960
SatI GCNGC 5 cut(s) 95, 806, 984, 1206, 1230
Sau3AI GATC 3 cut(s) 174, 316, 618
Sau96I GGNCC 5 cut(s) 169, 274, 729, 875, 1111
SchI GAGTC 4 cut(s) 875, 985, 1190, 1302
ScrFI CCNGG 3 cut(s) 6, 651, 1241
SduI GDGCHC 1 cut(s) 649
SfaNI GCATC 4 cut(s) 117, 644, 666, 897
SfcI CTRYAG 1 cut(s) 1206
SinI GGWCC 4 cut(s) 169, 274, 729, 1111
SmiMI CAYNNNNRTG 2 cut(s) 588, 652
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
SsiI CCGC 3 cut(s) 49, 806, 1193
SspMI CTAG 3 cut(s) 251, 314, 560
StyD4I CCNGG 3 cut(s) 4, 649, 1239
TaaI ACNGT 6 cut(s) 431, 946, 1010, 1088, 1171, 1264
TaiI ACGT 2 cut(s) 346, 609
TaqI TCGA 5 cut(s) 30, 153, 390, 442, 1296
TauI GCSGC 1 cut(s) 808
TfiI GAWTC 1 cut(s) 665
Tru1I TTAA 4 cut(s) 480, 674, 792, 960
Tru9I TTAA 4 cut(s) 480, 674, 792, 960
TscAI CASTG 4 cut(s) 17, 552, 1015, 1215
TseFI GTSAC 2 cut(s) 562, 576
TseI GCWGC 4 cut(s) 94, 983, 1205, 1229
Tsp45I GTSAC 2 cut(s) 562, 576
TspDTI ATGAA 2 cut(s) 51, 1179
TspGWI ACGGA 2 cut(s) 80, 854
TspRI CASTG 4 cut(s) 17, 552, 1015, 1215
Vha464I CTTAAG 1 cut(s) 479
VneI GTGCAC 1 cut(s) 645
VpaK11BI GGWCC 4 cut(s) 169, 274, 729, 1111
XagI CCTNNNNNAGG 1 cut(s) 639
XapI RAATTY 2 cut(s) 788, 813
XbaI TCTAGA 1 cut(s) 313
XceI RCATGY 1 cut(s) 570
XcmI CCANNNNNNNNNTGG 1 cut(s) 792
XmiI GTMKAC 1 cut(s) 357
XmnI GAANNNNTTC 1 cut(s) 1170
XspI CTAG 3 cut(s) 251, 314, 560
Zsp2I ATGCAT 1 cut(s) 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.