Prupe.6G364000_v2.0.a1

Phospholipase A(1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0005852
Physical Location & Seq
Forward (+)
30568263 .. 30573864
5602 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G364000.2

Sequence Viewer

Length: 1350 bp
ATGGGAGCGCGCTGCTTCTGCCCCTGTTTCGACTTCGACGACCACGACCACGACAATCGCACCGCCCAAACCGAGCTCGACCCCGTTCTACTCGTGTCCGGCATTGGAGGCTCCATTCTGCATTCCAAGAGGAAGAAGCTCGGGTTCGACACCCGGGTTTGGGTTCGGATCCTACTGGCCGACTTGGAGTTCAAGAAGAAGCTGTGGTCTGTCTATAATCCCCAAACAGGTTATACAGAAACACTAGACAAAGACACTGAAATCGTGGTCCCGGATGACGATTATGGGCTATACGCAATTGATATTCTAGATCCTTCTTGGTTTGTGAAATGCATACGTGTGAAAGAGGTATACCAGTTCCATGATATGATTGATATGCTAGTGGGATGTGGGTATAAGAAAGGAACCACTTTGTTTGGATATGGCTATGATTTCCGACAAAGCAATAGAATTGACAAGTTAATGGAAGGTCTTAAGGTGAAATTGGAAACTGCTTACAAGGCTTCTGGGGGTAGAAAAGTAAATATAATTTCACATTCAATGGGTGGCCTGCTAGTCACATGTTTTATGTCACTCCATAATGATGTATTTTTGAAGTATGTGAGTAAATGGATTTGCATAGCTTGCCCTTTTCAAGGTGCGCCAGGATGCATCAATGACTCTCTCTTAACTGGATTGCAGTTTGTTGAAGGCTTGGAGAGCTACTTTTTCGTATCAAGGTGGACTATGCACCAGCTGTTGGTTGAGTGCCCATCAATCTATGAGATGCTGGCAAATCCTAAATTTGATTGGAAAGAACTGCCAGAAATTCAGGTTTGGAGGAAGCATTCTAAGGATGGGGAAACTATTGTGGATCTGGAGTCTTATGGCCCAATTGAAAGCATATCTTTGTTTGAAGAAGCATTGAAACATAATGAGCTAAGTTATGATGGTAAAACGGTAGCTTTGCCATTTAACTTTTCTATTCTCAGATGGGCTGCTGAGACTCGCCAGGTTTTAAACAATGCTAAACTACCAGATGGAGTCTGCTTCTATAACATTTATGGAACATCATTCGACACACCTTTTGGTGTTTGCTATGGTTCGAAGACATCTCCAATTGAGGACTTGTCTGAAATATGCCATTCAATGCCTCAATATTCTTATGTGGATGGAGATGAAACAGTTCCGGCTGAGTCAGCAAAGGCAGATGGATTTGCTGCAGTTGAAAGAGTAGCAATAGCTGCAAGACACAGGGAACTATTGCGTGATAAAACAGTTTTTCAACATATCCAAAGGTGGTTGGGAGTTGAACAGAGGGTCAGCATACGTTCTAAGACATCCAGAGTTGCAGATGCCTCTTCAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko00592 ko01100 ko01110 map00564 map00592 map01100 map01110
Pfam Domains
Protein Families
LCAT

Protein Analysis

450

Amino Acids

51.01

Weight (kDa)

5.79

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 739
AccI GTMKAC 1 cut(s) 351
AccII CGCG 1 cut(s) 10
AciI CCGC 1 cut(s) 63
AclWI GGATC 4 cut(s) 163, 176, 305, 861
AcoI YGGCCR 1 cut(s) 177
AcsI RAATTY 2 cut(s) 782, 807
AfiI CCNNNNNNNGG 5 cut(s) 159, 160, 227, 635, 739
AflII CTTAAG 1 cut(s) 473
AflIII ACRYGT 2 cut(s) 337, 560
AjnI CCWGG 2 cut(s) 643, 990
AluBI AGCT 9 cut(s) 76, 139, 202, 623, 702, 736, 919, 944, 1223
AluI AGCT 9 cut(s) 76, 139, 202, 623, 702, 736, 919, 944, 1223
Alw21I GWGCWC 1 cut(s) 78
Alw26I GTCTC 1 cut(s) 977
AlwI GGATC 4 cut(s) 163, 176, 305, 861
Ama87I CYCGRG 2 cut(s) 140, 153
AoxI GGCC 3 cut(s) 177, 547, 868
ApeKI GCWGC 4 cut(s) 12, 977, 1199, 1223
ApoI RAATTY 2 cut(s) 782, 807
ArsI GACNNNNNNTTYG 2 cut(s) 1049, 1081
Asp700I GAANNNNTTC 1 cut(s) 1164
AspLEI GCGC 3 cut(s) 10, 12, 643
AspS9I GGNCC 2 cut(s) 268, 869
AsuC2I CCSGG 3 cut(s) 154, 155, 272
AsuHPI GGTGA 1 cut(s) 490
AsuII TTCGAA 1 cut(s) 1085
AvaI CYCGRG 2 cut(s) 140, 153
AvaII GGWCC 1 cut(s) 268
BaeGI GKGCMC 1 cut(s) 752
BamHI GGATCC 1 cut(s) 168
BanII GRGCYC 1 cut(s) 78
BauI CACGAG 1 cut(s) 92
BbsI GAAGAC 1 cut(s) 1094
Bbv12I GWGCWC 1 cut(s) 78
BbvI GCAGC 3 cut(s) 964, 1186, 1210
BccI CCATC 7 cut(s) 760, 830, 923, 966, 1013, 1145, 1184
BciT130I CCWGG 2 cut(s) 645, 992
BcnI CCSGG 3 cut(s) 154, 155, 272
BcoDI GTCTC 1 cut(s) 977
BfaI CTAG 4 cut(s) 245, 308, 380, 554
BfmI CTRYAG 1 cut(s) 1200
BfrI CTTAAG 1 cut(s) 473
BisI GCNGC 4 cut(s) 13, 978, 1200, 1224
BlsI GCNGC 4 cut(s) 14, 979, 1201, 1225
Bme1390I CCNGG 5 cut(s) 154, 155, 272, 645, 992
Bme18I GGWCC 1 cut(s) 268
BmeT110I CYCGRG 2 cut(s) 140, 153
BmgT120I GGNCC 2 cut(s) 268, 869
BmiI GGNNCC 4 cut(s) 112, 170, 270, 406
BmrFI CCNGG 5 cut(s) 154, 155, 272, 645, 992
BmsI GCATC 4 cut(s) 638, 660, 756, 1324
BpiI GAAGAC 1 cut(s) 1094
BpmI CTGGAG 1 cut(s) 878
Bpu14I TTCGAA 1 cut(s) 1085
BpuMI CCSGG 3 cut(s) 154, 155, 272
BsaAI YACGTR 1 cut(s) 338
BsaJI CCNNGG 1 cut(s) 153
Bsc4I CCNNNNNNNGG 5 cut(s) 159, 160, 227, 635, 739
Bse1I ACTGG 3 cut(s) 180, 355, 676
BseBI CCWGG 2 cut(s) 645, 992
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 6 cut(s) 280, 392, 653, 841, 1156, 1319
BseLI CCNNNNNNNGG 5 cut(s) 159, 160, 227, 635, 739
BseMII CTCAG 3 cut(s) 972, 982, 1164
BseNI ACTGG 3 cut(s) 180, 355, 676
BsePI GCGCGC 1 cut(s) 8
BseSI GKGCMC 1 cut(s) 752
BseXI GCAGC 3 cut(s) 964, 1186, 1210
Bsh1236I CGCG 1 cut(s) 10
BshFI GGCC 3 cut(s) 179, 549, 870
BsiHKAI GWGCWC 1 cut(s) 78
BsiHKCI CYCGRG 2 cut(s) 140, 153
BsiSI CCGG 4 cut(s) 99, 154, 272, 1169
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 5 cut(s) 159, 160, 227, 635, 739
BsmAI GTCTC 1 cut(s) 977
BsmFI GGGAC 1 cut(s) 254
BsmI GAATGC 2 cut(s) 121, 826
BsnI GGCC 3 cut(s) 179, 549, 870
BsoBI CYCGRG 2 cut(s) 140, 153
Bsp119I TTCGAA 1 cut(s) 1085
Bsp1286I GDGCHC 2 cut(s) 78, 752
Bsp143I GATC 3 cut(s) 168, 310, 853
BspACI CCGC 1 cut(s) 63
BspANI GGCC 3 cut(s) 179, 549, 870
BspCNI CTCAG 3 cut(s) 973, 981, 1165
BspFNI CGCG 1 cut(s) 10
BspLI GGNNCC 4 cut(s) 112, 170, 270, 406
BspMAI CTGCAG 1 cut(s) 1204
BspPI GGATC 4 cut(s) 163, 176, 305, 861
BspT104I TTCGAA 1 cut(s) 1085
BspTI CTTAAG 1 cut(s) 473
BsrI ACTGG 3 cut(s) 180, 355, 676
BssECI CCNNGG 1 cut(s) 153
BssHII GCGCGC 1 cut(s) 8
BssMI GATC 3 cut(s) 168, 310, 853
BssNAI GTATAC 1 cut(s) 352
BssSI CACGAG 1 cut(s) 92
Bst1107I GTATAC 1 cut(s) 352
Bst2BI CACGAG 1 cut(s) 92
Bst2UI CCWGG 2 cut(s) 645, 992
Bst4CI ACNGT 3 cut(s) 940, 1165, 1258
Bst6I CTCTTC 1 cut(s) 1345
BstAFI CTTAAG 1 cut(s) 473
BstAPI GCANNNNNTGC 2 cut(s) 624, 1223
BstBAI YACGTR 1 cut(s) 338
BstBI TTCGAA 1 cut(s) 1085
BstC8I GCNNGC 4 cut(s) 10, 551, 625, 771
BstDEI CTNAG 6 cut(s) 831, 920, 968, 981, 1173, 1314
BstENI CCTNNNNNAGG 1 cut(s) 633
BstF5I GGATG 6 cut(s) 280, 392, 653, 841, 1156, 1319
BstFNI CGCG 1 cut(s) 10
BstHHI GCGC 3 cut(s) 10, 12, 643
BstKTI GATC 3 cut(s) 171, 313, 856
BstMAI GTCTC 1 cut(s) 977
BstMBI GATC 3 cut(s) 168, 310, 853
BstMWI GCNNNNNNNGC 7 cut(s) 18, 108, 500, 624, 699, 1178, 1223
BstNI CCWGG 2 cut(s) 645, 992
BstNSI RCATGY 1 cut(s) 564
BstSCI CCNGG 5 cut(s) 152, 153, 270, 643, 990
BstSFI CTRYAG 1 cut(s) 1200
BstSLI GKGCMC 1 cut(s) 752
BstUI CGCG 1 cut(s) 10
BstV1I GCAGC 3 cut(s) 964, 1186, 1210
BstV2I GAAGAC 1 cut(s) 1094
BstX2I RGATCY 3 cut(s) 168, 310, 853
BstYI RGATCY 3 cut(s) 168, 310, 853
BstZ17I GTATAC 1 cut(s) 352
BsuRI GGCC 3 cut(s) 179, 549, 870
BtsCI GGATG 6 cut(s) 280, 392, 653, 841, 1156, 1319
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 4 cut(s) 10, 551, 625, 771
CfoI GCGC 3 cut(s) 10, 12, 643
Cfr13I GGNCC 2 cut(s) 268, 869
Cfr9I CCCGGG 1 cut(s) 153
CspCI CAANNNNNGTGG 2 cut(s) 397, 432
CviAII CATG 2 cut(s) 362, 561
DdeI CTNAG 6 cut(s) 831, 920, 968, 981, 1173, 1314
DpnI GATC 3 cut(s) 170, 312, 855
DpnII GATC 3 cut(s) 168, 310, 853
DraI TTTAAA 1 cut(s) 999
EaeI YGGCCR 1 cut(s) 177
Eam1104I CTCTTC 1 cut(s) 1345
EarI CTCTTC 1 cut(s) 1345
Ecl136II GAGCTC 1 cut(s) 76
Eco24I GRGCYC 1 cut(s) 78
Eco47I GGWCC 1 cut(s) 268
Eco53kI GAGCTC 1 cut(s) 76
Eco88I CYCGRG 2 cut(s) 140, 153
EcoICRI GAGCTC 1 cut(s) 76
EcoNI CCTNNNNNAGG 1 cut(s) 633
EcoRII CCWGG 2 cut(s) 643, 990
EcoT22I ATGCAT 2 cut(s) 335, 653
EcoT38I GRGCYC 1 cut(s) 78
FaeI CATG 2 cut(s) 365, 564
FalI AAGNNNNNCTT 2 cut(s) 871, 903
FaqI GGGAC 1 cut(s) 254
FatI CATG 2 cut(s) 361, 560
FblI GTMKAC 1 cut(s) 351
Fnu4HI GCNGC 4 cut(s) 13, 978, 1200, 1224
FokI GGATG 6 cut(s) 287, 399, 660, 848, 1163, 1306
FriOI GRGCYC 1 cut(s) 78
Fsp4HI GCNGC 4 cut(s) 13, 978, 1200, 1224
FspBI CTAG 4 cut(s) 245, 308, 380, 554
GlaI GCGC 3 cut(s) 9, 11, 642
GluI GCNGC 4 cut(s) 13, 978, 1200, 1224
GsuI CTGGAG 1 cut(s) 878
HaeIII GGCC 3 cut(s) 179, 549, 870
HapII CCGG 4 cut(s) 99, 154, 272, 1169
HhaI GCGC 3 cut(s) 10, 12, 643
Hin1II CATG 2 cut(s) 365, 564
Hin6I GCGC 3 cut(s) 8, 10, 641
HinP1I GCGC 3 cut(s) 8, 10, 641
HinfI GANTC 5 cut(s) 659, 860, 985, 1023, 1175
HpaII CCGG 4 cut(s) 99, 154, 272, 1169
HphI GGTGA 1 cut(s) 490
Hpy166II GTNNAC 2 cut(s) 352, 723
Hpy188I TCNGA 4 cut(s) 168, 437, 971, 1114
Hpy188III TCNNGA 4 cut(s) 193, 308, 857, 1323
Hpy8I GTNNAC 2 cut(s) 352, 723
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 3 cut(s) 324, 461, 683
HpyCH4III ACNGT 3 cut(s) 940, 1165, 1258
HpyCH4IV ACGT 2 cut(s) 337, 1309
HpyCH4V TGCA 9 cut(s) 121, 333, 618, 651, 679, 730, 1202, 1226, 1331
HpyF10VI GCNNNNNNNGC 7 cut(s) 18, 108, 500, 624, 699, 1178, 1223
HpyF3I CTNAG 6 cut(s) 831, 920, 968, 981, 1173, 1314
HpySE526I ACGT 2 cut(s) 337, 1309
Hsp92II CATG 2 cut(s) 365, 564
HspAI GCGC 3 cut(s) 8, 10, 641
Kzo9I GATC 3 cut(s) 168, 310, 853
LmnI GCTCC 2 cut(s) 5, 116
Lsp1109I GCAGC 3 cut(s) 964, 1186, 1210
LweI GCATC 4 cut(s) 638, 660, 756, 1324
MaeI CTAG 4 cut(s) 245, 308, 380, 554
MaeII ACGT 2 cut(s) 337, 1309
MaeIII GTNAC 2 cut(s) 556, 570
MalI GATC 3 cut(s) 170, 312, 855
MboI GATC 3 cut(s) 168, 310, 853
MboII GAAGA 5 cut(s) 145, 208, 908, 1099, 1332
MfeI CAATTG 3 cut(s) 297, 873, 1098
MflI RGATCY 3 cut(s) 168, 310, 853
MhlI GDGCHC 2 cut(s) 78, 752
MluCI AATT 8 cut(s) 297, 450, 482, 528, 782, 807, 873, 1098
MlyI GAGTC 5 cut(s) 653, 869, 979, 1032, 1184
MmeI TCCRAC 1 cut(s) 460
MnlI CCTC 8 cut(s) 101, 123, 340, 813, 1096, 1143, 1290, 1348
Mph1103I ATGCAT 2 cut(s) 335, 653
MroXI GAANNNNTTC 1 cut(s) 1164
MseI TTAA 5 cut(s) 461, 474, 668, 954, 998
MslI CAYNNNNRTG 2 cut(s) 338, 582
MspA1I CMGCKG 1 cut(s) 736
MspCI CTTAAG 1 cut(s) 473
MspI CCGG 4 cut(s) 99, 154, 272, 1169
MspR9I CCNGG 5 cut(s) 154, 155, 272, 645, 992
MunI CAATTG 3 cut(s) 297, 873, 1098
Mva1269I GAATGC 2 cut(s) 121, 826
MvaI CCWGG 2 cut(s) 645, 992
MvnI CGCG 1 cut(s) 10
MwoI GCNNNNNNNGC 7 cut(s) 18, 108, 500, 624, 699, 1178, 1223
NciI CCSGG 3 cut(s) 154, 155, 272
NdeII GATC 3 cut(s) 168, 310, 853
NlaIII CATG 2 cut(s) 365, 564
NlaIV GGNNCC 4 cut(s) 112, 170, 270, 406
NmuCI GTSAC 2 cut(s) 556, 570
NsiI ATGCAT 2 cut(s) 335, 653
NspI RCATGY 1 cut(s) 564
NspV TTCGAA 1 cut(s) 1085
PauI GCGCGC 1 cut(s) 8
PciI ACATGT 1 cut(s) 560
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 42
PctI GAATGC 2 cut(s) 121, 826
PdmI GAANNNNTTC 1 cut(s) 1164
PflMI CCANNNNNTGG 1 cut(s) 739
PfoI TCCNGGA 1 cut(s) 270
PkrI GCNGC 4 cut(s) 14, 979, 1201, 1225
PleI GAGTC 5 cut(s) 653, 868, 979, 1031, 1183
PpsI GAGTC 5 cut(s) 653, 868, 979, 1031, 1183
Ppu21I YACGTR 1 cut(s) 338
PscI ACATGT 1 cut(s) 560
Psp124BI GAGCTC 1 cut(s) 78
Psp6I CCWGG 2 cut(s) 643, 990
PspGI CCWGG 2 cut(s) 643, 990
PspN4I GGNNCC 4 cut(s) 112, 170, 270, 406
PspPI GGNCC 2 cut(s) 268, 869
PstI CTGCAG 1 cut(s) 1204
PsuI RGATCY 3 cut(s) 168, 310, 853
PteI GCGCGC 1 cut(s) 8
PvuII CAGCTG 1 cut(s) 736
RseI CAYNNNNRTG 2 cut(s) 338, 582
SacI GAGCTC 1 cut(s) 78
SaqAI TTAA 5 cut(s) 461, 474, 668, 954, 998
SatI GCNGC 4 cut(s) 13, 978, 1200, 1224
Sau3AI GATC 3 cut(s) 168, 310, 853
Sau96I GGNCC 2 cut(s) 268, 869
SchI GAGTC 5 cut(s) 653, 869, 979, 1032, 1184
ScrFI CCNGG 5 cut(s) 154, 155, 272, 645, 992
SduI GDGCHC 2 cut(s) 78, 752
SfaNI GCATC 4 cut(s) 638, 660, 756, 1324
SfcI CTRYAG 1 cut(s) 1200
SfuI TTCGAA 1 cut(s) 1085
SinI GGWCC 1 cut(s) 268
SmaI CCCGGG 1 cut(s) 155
SmiMI CAYNNNNRTG 2 cut(s) 338, 582
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
Sse9I AATT 8 cut(s) 297, 450, 482, 528, 782, 807, 873, 1098
SsiI CCGC 1 cut(s) 63
SspI AATATT 1 cut(s) 1139
SspMI CTAG 4 cut(s) 245, 308, 380, 554
SstI GAGCTC 1 cut(s) 78
StyD4I CCNGG 5 cut(s) 152, 153, 270, 643, 990
TaaI ACNGT 3 cut(s) 940, 1165, 1258
TaiI ACGT 2 cut(s) 340, 1312
TaqI TCGA 6 cut(s) 30, 36, 78, 147, 1056, 1085
TasI AATT 8 cut(s) 297, 450, 482, 528, 782, 807, 873, 1098
Tru1I TTAA 5 cut(s) 461, 474, 668, 954, 998
Tru9I TTAA 5 cut(s) 461, 474, 668, 954, 998
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 2 cut(s) 556, 570
TseI GCWGC 4 cut(s) 12, 977, 1199, 1223
Tsp45I GTSAC 2 cut(s) 556, 570
TspDTI ATGAA 1 cut(s) 1173
TspMI CCCGGG 1 cut(s) 153
TspRI CASTG 1 cut(s) 262
Van91I CCANNNNNTGG 1 cut(s) 739
Vha464I CTTAAG 1 cut(s) 473
VpaK11BI GGWCC 1 cut(s) 268
XagI CCTNNNNNAGG 1 cut(s) 633
XapI RAATTY 2 cut(s) 782, 807
XbaI TCTAGA 1 cut(s) 307
XceI RCATGY 1 cut(s) 564
XmaI CCCGGG 1 cut(s) 153
XmiI GTMKAC 1 cut(s) 351
XmnI GAANNNNTTC 1 cut(s) 1164
XspI CTAG 4 cut(s) 245, 308, 380, 554
Zsp2I ATGCAT 2 cut(s) 335, 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.