AT3G03330

dehydrogenase reductase SDR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007904
Physical Location & Seq
Reverse (-)
783332 .. 786209
2878 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G03330.1

Sequence Viewer

Length: 987 bp
ATGCTGACTCTACTCTTCTTCTCTCTCGGAGCGCTTCTTCTTATCGTTCTGCTCTTCAAATTTGCATTCGCCGATGGAGATTTTACCTTGATTTCGAAGAAGCATGTGAAGCGTGAGGCCATACAAGGCAAGGTCGTCTGGATCACAGGGGCTAGCCGTGGAATTGGTGAAATTCTTGCTAAACAGTTTGCAAGTTTAGATGCCAAGCTTATTCTCTCTGCTAGGAATAAAGCTGAACTGGATCGGGTTAAGAGTGAGCTTAAAGGTAAATTTGCACCAGAAGATGTCAAGGTTTTGCCTTTAGATTTAGCTAGCGGCGAAGAGTCACTCAAACATGTTGTAGAGCAAGCAGTGTCGCTTTTCCCTGGAGCTGGTGTTGATTATTTGGTTCACAATGCTGCCTTTGAGCGTCCGAAATCCAAGGCATCGGATGCAAGTGAGGAAACTCTCAAGACTACATTCGATGTTAATGTCTTTGGGACGATATCTCTGACAAAGTTGGTAGCTCCTCATATGCTAAAACAAGGAGGGGGTCATTTTGTTGTGATAAGCAGTGCTGCAGGAAAGGTACCATCACCTGGACAGGCTATATATTCTGCTTCAAAACATGCTCTTCATGGCTACTTCCACAGCTTGCGTTCTGAGTTCTGTCAGAAGGGAATCAAGGTTACCGTCGTTTGTCCTGGTCCAATAGAAACCTGGAATGGTACAGGAACATCAACTTCGGAAGACAGCAAGTCTCCTGAGAAACGTGTGTCATCTGAACGATGTGCAGAGCTGACTATAATCGCTGCATCTCATAACTTGAAGGAAGCTTGGATTTCATATCAGCCAGTACTACTCGTGATGTATCTAGTGCAGTACATGCCTTCCCTTGGCTTCTGGCTCATGGACAAGGTTGGAGGAAAGCGTGTGGAGGTCGCGGAAAAGAAAGGCAACACATACTCGTGGGATTTACTGTTCGGGAAGAAGACTAAAACAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

328

Amino Acids

35.79

Weight (kDa)

9.26

Isoelectric Point (pI)

32.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 44 - 235 9.6e-50 short chain dehydrogenase
KR PF08659 46 - 215 4.3e-12 KR domain
adh_short_C2 PF13561 50 - 247 2.4e-36 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 568
AccB1I GGYRCC 1 cut(s) 568
AccB7I CCANNNNNTGG 1 cut(s) 578
AccII CGCG 1 cut(s) 923
AciI CCGC 2 cut(s) 315, 923
AclWI GGATC 2 cut(s) 149, 249
AcsI RAATTY 3 cut(s) 59, 171, 269
AfaI GTAC 4 cut(s) 570, 709, 837, 863
AfeI AGCGCT 1 cut(s) 33
AfiI CCNNNNNNNGG 3 cut(s) 371, 578, 875
AflIII ACRYGT 2 cut(s) 334, 751
AgsI TTSAA 3 cut(s) 58, 603, 808
AhdI GACNNNNNGTC 1 cut(s) 736
AjnI CCWGG 4 cut(s) 364, 577, 682, 698
AloI GAACNNNNNNTCC 2 cut(s) 944, 976
AluBI AGCT 9 cut(s) 208, 233, 259, 311, 371, 506, 633, 778, 815
AluI AGCT 9 cut(s) 208, 233, 259, 311, 371, 506, 633, 778, 815
Alw26I GTCTC 1 cut(s) 744
AlwI GGATC 2 cut(s) 149, 249
Aor51HI AGCGCT 1 cut(s) 33
AoxI GGCC 1 cut(s) 117
ApeKI GCWGC 3 cut(s) 398, 557, 791
ApoI RAATTY 3 cut(s) 59, 171, 269
Asp718I GGTACC 1 cut(s) 568
AspLEI GCGC 1 cut(s) 34
AspS9I GGNCC 1 cut(s) 686
AsuHPI GGTGA 2 cut(s) 179, 567
AsuII TTCGAA 1 cut(s) 95
AsuNHI GCTAGC 2 cut(s) 152, 311
AvaII GGWCC 1 cut(s) 686
BanI GGYRCC 1 cut(s) 568
BauI CACGAG 2 cut(s) 842, 946
BbsI GAAGAC 2 cut(s) 735, 977
BbvI GCAGC 3 cut(s) 385, 544, 778
BccI CCATC 2 cut(s) 68, 580
BceAI ACGGC 1 cut(s) 141
BciT130I CCWGG 4 cut(s) 366, 579, 684, 700
BcoDI GTCTC 1 cut(s) 744
BfaI CTAG 4 cut(s) 153, 222, 312, 854
BfmI CTRYAG 1 cut(s) 558
BfoI RGCGCY 1 cut(s) 35
BisI GCNGC 4 cut(s) 316, 399, 558, 792
BlsI GCNGC 4 cut(s) 317, 400, 559, 793
BmcAI AGTACT 1 cut(s) 837
Bme1390I CCNGG 4 cut(s) 366, 579, 684, 700
Bme18I GGWCC 1 cut(s) 686
BmeRI GACNNNNNGTC 1 cut(s) 736
BmgT120I GGNCC 1 cut(s) 686
BmiI GGNNCC 1 cut(s) 570
BmrFI CCNGG 4 cut(s) 366, 579, 684, 700
BmsI GCATC 4 cut(s) 190, 421, 434, 803
BmtI GCTAGC 2 cut(s) 156, 315
BpiI GAAGAC 2 cut(s) 735, 977
BpmI CTGGAG 1 cut(s) 387
Bpu14I TTCGAA 1 cut(s) 95
BpuEI CTTGAG 1 cut(s) 434
BsaJI CCNNGG 4 cut(s) 157, 364, 420, 874
BsaXI ACNNNNNCTCC 2 cut(s) 360, 390
Bsc4I CCNNNNNNNGG 3 cut(s) 371, 578, 875
Bse1I ACTGG 2 cut(s) 243, 833
BseBI CCWGG 4 cut(s) 366, 579, 684, 700
BseDI CCNNGG 4 cut(s) 157, 364, 420, 874
BseGI GGATG 1 cut(s) 436
BseLI CCNNNNNNNGG 3 cut(s) 371, 578, 875
BseMII CTCAG 2 cut(s) 633, 735
BseNI ACTGG 2 cut(s) 243, 833
BseRI GAGGAG 1 cut(s) 498
BseXI GCAGC 3 cut(s) 385, 544, 778
BsgI GTGCAG 2 cut(s) 792, 878
Bsh1236I CGCG 1 cut(s) 923
BshFI GGCC 1 cut(s) 119
BshNI GGYRCC 1 cut(s) 568
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 3 cut(s) 371, 578, 875
BsmAI GTCTC 1 cut(s) 744
BsmFI GGGAC 1 cut(s) 493
BsmI GAATGC 1 cut(s) 65
BsnI GGCC 1 cut(s) 119
Bsp119I TTCGAA 1 cut(s) 95
Bsp143I GATC 2 cut(s) 141, 241
BspACI CCGC 2 cut(s) 315, 923
BspANI GGCC 1 cut(s) 119
BspCNI CTCAG 2 cut(s) 634, 736
BspFNI CGCG 1 cut(s) 923
BspLI GGNNCC 1 cut(s) 570
BspMAI CTGCAG 1 cut(s) 562
BspOI GCTAGC 2 cut(s) 156, 315
BspPI GGATC 2 cut(s) 149, 249
BspQI GCTCTTC 2 cut(s) 59, 618
BspT104I TTCGAA 1 cut(s) 95
BspT107I GGYRCC 1 cut(s) 568
BsrI ACTGG 2 cut(s) 243, 833
BssECI CCNNGG 4 cut(s) 157, 364, 420, 874
BssMI GATC 2 cut(s) 141, 241
BssSI CACGAG 2 cut(s) 842, 946
BssT1I CCWWGG 2 cut(s) 420, 874
Bst2BI CACGAG 2 cut(s) 842, 946
Bst2UI CCWGG 4 cut(s) 366, 579, 684, 700
Bst4CI ACNGT 3 cut(s) 186, 673, 960
Bst6I CTCTTC 4 cut(s) 20, 59, 315, 618
BstAPI GCANNNNNTGC 2 cut(s) 431, 865
BstBI TTCGAA 1 cut(s) 95
BstC8I GCNNGC 4 cut(s) 154, 313, 348, 635
BstDEI CTNAG 2 cut(s) 642, 744
BstDSI CCRYGG 1 cut(s) 157
BstEII GGTNACC 1 cut(s) 667
BstF5I GGATG 1 cut(s) 436
BstFNI CGCG 1 cut(s) 923
BstH2I RGCGCY 1 cut(s) 35
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 2 cut(s) 144, 244
BstMAI GTCTC 1 cut(s) 744
BstMBI GATC 2 cut(s) 141, 241
BstMWI GCNNNNNNNGC 3 cut(s) 109, 431, 865
BstNI CCWGG 4 cut(s) 366, 579, 684, 700
BstNSI RCATGY 4 cut(s) 107, 338, 611, 868
BstPI GGTNACC 1 cut(s) 667
BstSCI CCNGG 4 cut(s) 364, 577, 682, 698
BstSFI CTRYAG 1 cut(s) 558
BstUI CGCG 1 cut(s) 923
BstV1I GCAGC 3 cut(s) 385, 544, 778
BstV2I GAAGAC 2 cut(s) 735, 977
BsuRI GGCC 1 cut(s) 119
BtgI CCRYGG 1 cut(s) 157
BtsCI GGATG 1 cut(s) 436
BtsI GCAGTG 2 cut(s) 357, 559
BtsIMutI CAGTG 2 cut(s) 357, 559
Cac8I GCNNGC 4 cut(s) 154, 313, 348, 635
CfoI GCGC 1 cut(s) 34
Cfr13I GGNCC 1 cut(s) 686
CseI GACGC 1 cut(s) 398
Csp6I GTAC 4 cut(s) 569, 708, 836, 862
CviAII CATG 6 cut(s) 104, 335, 608, 617, 865, 889
CviQI GTAC 4 cut(s) 569, 708, 836, 862
DdeI CTNAG 2 cut(s) 642, 744
DpnI GATC 2 cut(s) 143, 243
DpnII GATC 2 cut(s) 141, 241
DriI GACNNNNNGTC 1 cut(s) 736
Eam1104I CTCTTC 4 cut(s) 20, 59, 315, 618
Eam1105I GACNNNNNGTC 1 cut(s) 736
EarI CTCTTC 4 cut(s) 20, 59, 315, 618
Eco130I CCWWGG 2 cut(s) 420, 874
Eco32I GATATC 1 cut(s) 486
Eco47I GGWCC 1 cut(s) 686
Eco47III AGCGCT 1 cut(s) 33
Eco91I GGTNACC 1 cut(s) 667
EcoO65I GGTNACC 1 cut(s) 667
EcoRII CCWGG 4 cut(s) 364, 577, 682, 698
EcoRV GATATC 1 cut(s) 486
EcoT14I CCWWGG 2 cut(s) 420, 874
ErhI CCWWGG 2 cut(s) 420, 874
FaeI CATG 6 cut(s) 107, 338, 611, 620, 868, 892
FaqI GGGAC 1 cut(s) 493
FatI CATG 6 cut(s) 103, 334, 607, 616, 864, 888
FauNDI CATATG 1 cut(s) 513
Fnu4HI GCNGC 4 cut(s) 316, 399, 558, 792
FokI GGATG 1 cut(s) 443
Fsp4HI GCNGC 4 cut(s) 316, 399, 558, 792
FspBI CTAG 4 cut(s) 153, 222, 312, 854
GlaI GCGC 1 cut(s) 33
GluI GCNGC 4 cut(s) 316, 399, 558, 792
GsuI CTGGAG 1 cut(s) 387
HaeII RGCGCY 1 cut(s) 35
HaeIII GGCC 1 cut(s) 119
HgaI GACGC 1 cut(s) 398
HhaI GCGC 1 cut(s) 34
Hin1II CATG 6 cut(s) 107, 338, 611, 620, 868, 892
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HindIII AAGCTT 2 cut(s) 206, 813
HinfI GANTC 3 cut(s) 7, 323, 660
HphI GGTGA 2 cut(s) 179, 567
Hpy166II GTNNAC 1 cut(s) 391
Hpy188I TCNGA 8 cut(s) 29, 414, 430, 492, 643, 654, 727, 763
Hpy188III TCNNGA 5 cut(s) 139, 451, 743, 844, 964
Hpy8I GTNNAC 1 cut(s) 391
Hpy99I CGWCG 1 cut(s) 677
HpyAV CCTTC 3 cut(s) 649, 802, 879
HpyCH4III ACNGT 3 cut(s) 186, 673, 960
HpyCH4IV ACGT 1 cut(s) 751
HpyCH4V TGCA 8 cut(s) 65, 191, 275, 434, 560, 773, 794, 859
HpyF10VI GCNNNNNNNGC 3 cut(s) 109, 431, 865
HpyF3I CTNAG 2 cut(s) 642, 744
HpySE526I ACGT 1 cut(s) 751
Hsp92II CATG 6 cut(s) 107, 338, 611, 620, 868, 892
HspAI GCGC 1 cut(s) 32
KpnI GGTACC 1 cut(s) 572
Kzo9I GATC 2 cut(s) 141, 241
LguI GCTCTTC 2 cut(s) 59, 618
LmnI GCTCC 3 cut(s) 29, 368, 511
Lsp1109I GCAGC 3 cut(s) 385, 544, 778
LweI GCATC 4 cut(s) 190, 421, 434, 803
MaeI CTAG 4 cut(s) 153, 222, 312, 854
MaeII ACGT 1 cut(s) 751
MaeIII GTNAC 2 cut(s) 324, 667
MalI GATC 2 cut(s) 143, 243
MboI GATC 2 cut(s) 141, 241
MluCI AATT 4 cut(s) 59, 162, 171, 269
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 1 cut(s) 880
MnlI CCTC 6 cut(s) 109, 433, 519, 521, 896, 910
MseI TTAA 3 cut(s) 249, 261, 468
MslI CAYNNNNRTG 1 cut(s) 946
MspR9I CCNGG 4 cut(s) 366, 579, 684, 700
Mva1269I GAATGC 1 cut(s) 65
MvaI CCWGG 4 cut(s) 366, 579, 684, 700
MvnI CGCG 1 cut(s) 923
MwoI GCNNNNNNNGC 3 cut(s) 109, 431, 865
NdeI CATATG 1 cut(s) 513
NdeII GATC 2 cut(s) 141, 241
NheI GCTAGC 2 cut(s) 152, 311
NlaIII CATG 6 cut(s) 107, 338, 611, 620, 868, 892
NlaIV GGNNCC 1 cut(s) 570
NmuCI GTSAC 1 cut(s) 324
NspI RCATGY 4 cut(s) 107, 338, 611, 868
NspV TTCGAA 1 cut(s) 95
PciI ACATGT 1 cut(s) 334
PciSI GCTCTTC 2 cut(s) 59, 618
PctI GAATGC 1 cut(s) 65
PfeI GAWTC 1 cut(s) 660
PflMI CCANNNNNTGG 1 cut(s) 578
PkrI GCNGC 4 cut(s) 317, 400, 559, 793
PleI GAGTC 1 cut(s) 331
PpsI GAGTC 1 cut(s) 331
PscI ACATGT 1 cut(s) 334
Psp6I CCWGG 4 cut(s) 364, 577, 682, 698
PspEI GGTNACC 1 cut(s) 667
PspGI CCWGG 4 cut(s) 364, 577, 682, 698
PspN4I GGNNCC 1 cut(s) 570
PspPI GGNCC 1 cut(s) 686
PstI CTGCAG 1 cut(s) 562
RsaI GTAC 4 cut(s) 570, 709, 837, 863
RsaNI GTAC 4 cut(s) 569, 708, 836, 862
RseI CAYNNNNRTG 1 cut(s) 946
SapI GCTCTTC 2 cut(s) 59, 618
SaqAI TTAA 3 cut(s) 249, 261, 468
SatI GCNGC 4 cut(s) 316, 399, 558, 792
Sau3AI GATC 2 cut(s) 141, 241
Sau96I GGNCC 1 cut(s) 686
ScaI AGTACT 1 cut(s) 837
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 4 cut(s) 366, 579, 684, 700
SfaNI GCATC 4 cut(s) 190, 421, 434, 803
SfcI CTRYAG 1 cut(s) 558
SfuI TTCGAA 1 cut(s) 95
SinI GGWCC 1 cut(s) 686
SmiMI CAYNNNNRTG 1 cut(s) 946
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 4 cut(s) 59, 162, 171, 269
SsiI CCGC 2 cut(s) 315, 923
SspMI CTAG 4 cut(s) 153, 222, 312, 854
StyD4I CCNGG 4 cut(s) 364, 577, 682, 698
StyI CCWWGG 2 cut(s) 420, 874
TaaI ACNGT 3 cut(s) 186, 673, 960
TaiI ACGT 1 cut(s) 754
TaqI TCGA 2 cut(s) 95, 462
TasI AATT 4 cut(s) 59, 162, 171, 269
TatI WGTACW 2 cut(s) 835, 861
TauI GCSGC 1 cut(s) 318
TfiI GAWTC 1 cut(s) 660
Tru1I TTAA 3 cut(s) 249, 261, 468
Tru9I TTAA 3 cut(s) 249, 261, 468
TscAI CASTG 2 cut(s) 357, 559
TseFI GTSAC 1 cut(s) 324
TseI GCWGC 3 cut(s) 398, 557, 791
Tsp45I GTSAC 1 cut(s) 324
TspDTI ATGAA 2 cut(s) 605, 813
TspRI CASTG 2 cut(s) 357, 559
Van91I CCANNNNNTGG 1 cut(s) 578
VpaK11BI GGWCC 1 cut(s) 686
XapI RAATTY 3 cut(s) 59, 171, 269
XceI RCATGY 4 cut(s) 107, 338, 611, 868
XcmI CCANNNNNNNNNTGG 1 cut(s) 696
XspI CTAG 4 cut(s) 153, 222, 312, 854
ZrmI AGTACT 1 cut(s) 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.