Rorug02G0603200

Dehydrogenase reductase SDR family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007904
Physical Location & Seq
Reverse (-)
72177256 .. 72178159
904 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0603200.1

Sequence Viewer

Length: 822 bp
ATGCCGGGGCAGAAGATTGAAACGGGTCACCAGGATGTTGTTCATGATGTTGCCATGGATTACTATGGAAAGCGTCTAGCATCAGCTTCATCTGATATGACCATTAAGATAATTGGTGTCGGCAGCAATGCGGGTTCACAGCACCTTGCTACATTGTCCGGTCACAGAGGTCCTGTATGGCAGGTGGGTTGGGCGCATCCCAAGTTTGGGCCGATCCTTGCTTCGTGCTCGTATGATGGCCAAGTGATAATTTGGAAGGAGGGTAATCCAAATGAGTGGCAACAGGCTCATGTTTTCAATGAGCACAGTTCATCTGTAAATTCCATTTGTTGGGCGCCTCATGAACTTGGCCTCATCTTAGCTTGTGGTTCTTCTGATGGGAACATCTCTGTTTTCACTGCTAGGGCAGATGGTGGTTGGGACACCACTAGGATAGACCAAGCACACCCTGTTGGAAAGCTGGTCTCTGGTGGCTGCGACAATACAGTGAAGGTGTGGAAATTGTACAATGAGACTTGGAAGATGGATTGCTTCCCTGCTCTTCAAATGCACACTGATTGGGTGAGAGACGTAGCCTGGGCACCCAACTTGGGACTTCCCAAGTCCACCATTGCAAGTTGTTCACAGGACGGAACAGTGGTTATCTGGACTGTGGCCAAAGAAGGCGATCGATGGCAGGGGAAGGTACTGAAGGATTTTAAGACCCCTGTTTGGAGGGTGTCATGGTCACTAACAGGAAACCTACTGGCCGTGGCCGATGGAAATAGGTTTTCTCTTTATTTCTACTTTCCAGTTAATGTCATTAGTGTTCTTGTGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

273

Amino Acids

30.01

Weight (kDa)

6.54

Isoelectric Point (pI)

31.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TEP1_2nd PF25047 6 - 112 1.9e-06 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 8 - 136 2.1e-15 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 8 - 132 2.8e-14 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 9 - 135 1.7e-11 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 10 - 85 3.3e-12 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 11 - 132 2e-12 WDHD1 first WD40 domain
WD40_Prp19 PF24814 44 - 258 1.9e-16 Prp19 WD40 domain
WD40 PF00400 47 - 85 3.5e-06 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 70 - 137 3.1e-08 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 94 - 253 2.3e-11 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 145 - 260 2.5e-07 WDHD1 first WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 147 - 223 1.2e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 153 - 255 2.4e-13 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 153 - 250 6.3e-11 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 172
Acc36I ACCTGC 1 cut(s) 172
AccB1I GGYRCC 2 cut(s) 334, 580
AccB7I CCANNNNNTGG 1 cut(s) 330
AciI CCGC 1 cut(s) 131
AclWI GGATC 1 cut(s) 208
AcoI YGGCCR 4 cut(s) 238, 654, 747, 753
AcsI RAATTY 1 cut(s) 319
AcuI CTGAAG 1 cut(s) 710
AcyI GRCGYC 1 cut(s) 335
AfaI GTAC 2 cut(s) 506, 687
AfiI CCNNNNNNNGG 5 cut(s) 206, 207, 330, 590, 711
AgsI TTSAA 3 cut(s) 20, 298, 545
AjnI CCWGG 2 cut(s) 30, 575
AluBI AGCT 3 cut(s) 86, 362, 460
AluI AGCT 3 cut(s) 86, 362, 460
Alw21I GWGCWC 2 cut(s) 230, 306
Alw26I GTCTC 3 cut(s) 469, 506, 561
AlwI GGATC 1 cut(s) 208
AoxI GGCC 6 cut(s) 209, 238, 349, 654, 747, 753
ApeKI GCWGC 2 cut(s) 123, 474
ApoI RAATTY 1 cut(s) 319
AspLEI GCGC 2 cut(s) 196, 337
AspS9I GGNCC 2 cut(s) 170, 209
AsuC2I CCSGG 1 cut(s) 6
AsuHPI GGTGA 2 cut(s) 20, 574
AvaII GGWCC 1 cut(s) 170
BaeGI GKGCMC 1 cut(s) 583
BalI TGGCCA 2 cut(s) 240, 656
BanI GGYRCC 2 cut(s) 334, 580
Bbv12I GWGCWC 2 cut(s) 230, 306
BbvI GCAGC 2 cut(s) 135, 461
BccI CCATC 6 cut(s) 230, 371, 404, 517, 666, 752
BceAI ACGGC 1 cut(s) 734
BciT130I CCWGG 2 cut(s) 32, 577
BcnI CCSGG 1 cut(s) 6
BcoDI GTCTC 3 cut(s) 469, 506, 561
BfaI CTAG 4 cut(s) 77, 402, 429, 820
BfoI RGCGCY 1 cut(s) 338
BfuAI ACCTGC 1 cut(s) 172
BisI GCNGC 2 cut(s) 124, 475
BlsI GCNGC 2 cut(s) 125, 476
Bme1390I CCNGG 3 cut(s) 6, 32, 577
Bme18I GGWCC 1 cut(s) 170
BmgT120I GGNCC 2 cut(s) 170, 209
BmiI GGNNCC 2 cut(s) 336, 582
BmrFI CCNGG 3 cut(s) 6, 32, 577
BmsI GCATC 2 cut(s) 89, 205
BpuMI CCSGG 1 cut(s) 6
Bsa29I ATCGAT 1 cut(s) 670
BsaHI GRCGYC 1 cut(s) 335
BsaI GGTCTC 1 cut(s) 469
BsaJI CCNNGG 4 cut(s) 5, 54, 576, 750
BsaWI WCCGGW 1 cut(s) 158
Bsc4I CCNNNNNNNGG 5 cut(s) 206, 207, 330, 590, 711
Bse1I ACTGG 2 cut(s) 750, 791
Bse3DI GCAATG 2 cut(s) 133, 609
BseBI CCWGG 2 cut(s) 32, 577
BseCI ATCGAT 1 cut(s) 670
BseDI CCNNGG 4 cut(s) 5, 54, 576, 750
BseGI GGATG 2 cut(s) 40, 196
BseLI CCNNNNNNNGG 5 cut(s) 206, 207, 330, 590, 711
BseMI GCAATG 2 cut(s) 133, 609
BseNI ACTGG 2 cut(s) 750, 791
BseSI GKGCMC 1 cut(s) 583
BseXI GCAGC 2 cut(s) 135, 461
Bsh1285I CGRYCG 1 cut(s) 670
BshFI GGCC 6 cut(s) 211, 240, 351, 656, 749, 755
BshNI GGYRCC 2 cut(s) 334, 580
BshVI ATCGAT 1 cut(s) 670
BsiEI CGRYCG 1 cut(s) 670
BsiHKAI GWGCWC 2 cut(s) 230, 306
BsiSI CCGG 2 cut(s) 5, 159
BslFI GGGAC 2 cut(s) 434, 606
BslI CCNNNNNNNGG 5 cut(s) 206, 207, 330, 590, 711
BsmAI GTCTC 3 cut(s) 469, 506, 561
BsmBI CGTCTC 1 cut(s) 561
BsmFI GGGAC 2 cut(s) 434, 606
BsnI GGCC 6 cut(s) 211, 240, 351, 656, 749, 755
Bso31I GGTCTC 1 cut(s) 469
Bsp1286I GDGCHC 3 cut(s) 230, 306, 583
Bsp1407I TGTACA 1 cut(s) 504
Bsp143I GATC 2 cut(s) 213, 667
Bsp19I CCATGG 1 cut(s) 54
BspACI CCGC 1 cut(s) 131
BspANI GGCC 6 cut(s) 211, 240, 351, 656, 749, 755
BspDI ATCGAT 1 cut(s) 670
BspHI TCATGA 2 cut(s) 43, 340
BspLI GGNNCC 2 cut(s) 336, 582
BspMI ACCTGC 1 cut(s) 172
BspPI GGATC 1 cut(s) 208
BspQI GCTCTTC 1 cut(s) 546
BspT107I GGYRCC 2 cut(s) 334, 580
BspTNI GGTCTC 1 cut(s) 469
BsrDI GCAATG 2 cut(s) 133, 609
BsrGI TGTACA 1 cut(s) 504
BsrI ACTGG 2 cut(s) 750, 791
BssECI CCNNGG 4 cut(s) 5, 54, 576, 750
BssMI GATC 2 cut(s) 213, 667
BssNI GRCGYC 1 cut(s) 335
BssT1I CCWWGG 1 cut(s) 54
Bst2UI CCWGG 2 cut(s) 32, 577
Bst4CI ACNGT 4 cut(s) 308, 487, 637, 652
Bst6I CTCTTC 1 cut(s) 546
BstACI GRCGYC 1 cut(s) 335
BstAUI TGTACA 1 cut(s) 504
BstDEI CTNAG 1 cut(s) 358
BstDSI CCRYGG 2 cut(s) 54, 750
BstEII GGTNACC 1 cut(s) 26
BstF5I GGATG 2 cut(s) 40, 196
BstH2I RGCGCY 1 cut(s) 338
BstHHI GCGC 2 cut(s) 196, 337
BstKTI GATC 2 cut(s) 216, 670
BstMAI GTCTC 3 cut(s) 469, 506, 561
BstMBI GATC 2 cut(s) 213, 667
BstMCI CGRYCG 1 cut(s) 670
BstNI CCWGG 2 cut(s) 32, 577
BstPI GGTNACC 1 cut(s) 26
BstSCI CCNGG 3 cut(s) 4, 30, 575
BstSLI GKGCMC 1 cut(s) 583
BstV1I GCAGC 2 cut(s) 135, 461
BstXI CCANNNNNNTGG 1 cut(s) 276
Bsu15I ATCGAT 1 cut(s) 670
BsuRI GGCC 6 cut(s) 211, 240, 351, 656, 749, 755
BsuTUI ATCGAT 1 cut(s) 670
BtgI CCRYGG 2 cut(s) 54, 750
BtsCI GGATG 2 cut(s) 40, 196
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 4 cut(s) 396, 492, 552, 642
BveI ACCTGC 1 cut(s) 172
CciI TCATGA 2 cut(s) 43, 340
CfoI GCGC 2 cut(s) 196, 337
Cfr13I GGNCC 2 cut(s) 170, 209
ClaI ATCGAT 1 cut(s) 670
CseI GACGC 1 cut(s) 62
Csp6I GTAC 2 cut(s) 505, 686
CviAII CATG 5 cut(s) 44, 55, 290, 341, 723
CviQI GTAC 2 cut(s) 505, 686
DdeI CTNAG 1 cut(s) 358
DinI GGCGCC 1 cut(s) 336
DpnI GATC 2 cut(s) 215, 669
DpnII GATC 2 cut(s) 213, 667
EaeI YGGCCR 4 cut(s) 238, 654, 747, 753
Eam1104I CTCTTC 1 cut(s) 546
EarI CTCTTC 1 cut(s) 546
Eco130I CCWWGG 1 cut(s) 54
Eco31I GGTCTC 1 cut(s) 469
Eco47I GGWCC 1 cut(s) 170
Eco57I CTGAAG 1 cut(s) 710
Eco91I GGTNACC 1 cut(s) 26
EcoO109I RGGNCCY 1 cut(s) 170
EcoO65I GGTNACC 1 cut(s) 26
EcoRII CCWGG 2 cut(s) 30, 575
EcoT14I CCWWGG 1 cut(s) 54
EgeI GGCGCC 1 cut(s) 336
EheI GGCGCC 1 cut(s) 336
ErhI CCWWGG 1 cut(s) 54
Esp3I CGTCTC 1 cut(s) 561
FaeI CATG 5 cut(s) 47, 58, 293, 344, 726
FaiI YATR 9 cut(s) 45, 56, 66, 98, 178, 234, 291, 342, 724
FaqI GGGAC 2 cut(s) 434, 606
FatI CATG 5 cut(s) 43, 54, 289, 340, 722
FauI CCCGC 1 cut(s) 124
Fnu4HI GCNGC 2 cut(s) 124, 475
FokI GGATG 2 cut(s) 47, 183
Fsp4HI GCNGC 2 cut(s) 124, 475
FspBI CTAG 4 cut(s) 77, 402, 429, 820
GlaI GCGC 2 cut(s) 195, 336
GluI GCNGC 2 cut(s) 124, 475
HaeII RGCGCY 1 cut(s) 338
HaeIII GGCC 6 cut(s) 211, 240, 351, 656, 749, 755
HapII CCGG 2 cut(s) 5, 159
HgaI GACGC 1 cut(s) 62
HhaI GCGC 2 cut(s) 196, 337
Hin1I GRCGYC 1 cut(s) 335
Hin1II CATG 5 cut(s) 47, 58, 293, 344, 726
Hin6I GCGC 2 cut(s) 194, 335
HinP1I GCGC 2 cut(s) 194, 335
HpaII CCGG 2 cut(s) 5, 159
HphI GGTGA 2 cut(s) 20, 574
Hpy166II GTNNAC 4 cut(s) 137, 606, 623, 817
Hpy188I TCNGA 2 cut(s) 94, 376
Hpy188III TCNNGA 3 cut(s) 44, 341, 646
Hpy8I GTNNAC 4 cut(s) 137, 606, 623, 817
HpyAV CCTTC 5 cut(s) 250, 484, 656, 676, 685
HpyCH4III ACNGT 4 cut(s) 308, 487, 637, 652
HpyCH4IV ACGT 1 cut(s) 570
HpyCH4V TGCA 2 cut(s) 550, 614
HpyF3I CTNAG 1 cut(s) 358
HpySE526I ACGT 1 cut(s) 570
Hsp92I GRCGYC 1 cut(s) 335
Hsp92II CATG 5 cut(s) 47, 58, 293, 344, 726
HspAI GCGC 2 cut(s) 194, 335
KasI GGCGCC 1 cut(s) 334
Kzo9I GATC 2 cut(s) 213, 667
LguI GCTCTTC 1 cut(s) 546
Lsp1109I GCAGC 2 cut(s) 135, 461
LweI GCATC 2 cut(s) 89, 205
MaeI CTAG 4 cut(s) 77, 402, 429, 820
MaeII ACGT 1 cut(s) 570
MaeIII GTNAC 3 cut(s) 26, 161, 726
MalI GATC 2 cut(s) 215, 669
MboI GATC 2 cut(s) 213, 667
MboII GAAGA 4 cut(s) 25, 363, 532, 533
MhlI GDGCHC 3 cut(s) 230, 306, 583
MlsI TGGCCA 2 cut(s) 240, 656
MluCI AATT 4 cut(s) 111, 249, 319, 500
MluNI TGGCCA 2 cut(s) 240, 656
Mly113I GGCGCC 1 cut(s) 335
MmeI TCCRAC 1 cut(s) 433
MnlI CCTC 5 cut(s) 161, 253, 348, 362, 708
Mox20I TGGCCA 2 cut(s) 240, 656
MscI TGGCCA 2 cut(s) 240, 656
MseI TTAA 3 cut(s) 105, 699, 795
MslI CAYNNNNRTG 1 cut(s) 33
Msp20I TGGCCA 2 cut(s) 240, 656
MspI CCGG 2 cut(s) 5, 159
MspR9I CCNGG 3 cut(s) 6, 32, 577
MvaI CCWGG 2 cut(s) 32, 577
NarI GGCGCC 1 cut(s) 335
NciI CCSGG 1 cut(s) 6
NcoI CCATGG 1 cut(s) 54
NdeII GATC 2 cut(s) 213, 667
NlaIII CATG 5 cut(s) 47, 58, 293, 344, 726
NlaIV GGNNCC 2 cut(s) 336, 582
NmuCI GTSAC 3 cut(s) 26, 161, 726
PagI TCATGA 2 cut(s) 43, 340
PaqCI CACCTGC 1 cut(s) 172
PciSI GCTCTTC 1 cut(s) 546
PflMI CCANNNNNTGG 1 cut(s) 330
PkrI GCNGC 2 cut(s) 125, 476
Ple19I CGATCG 1 cut(s) 670
PluTI GGCGCC 1 cut(s) 338
PpuMI RGGWCCY 1 cut(s) 170
Psp5II RGGWCCY 1 cut(s) 170
Psp6I CCWGG 2 cut(s) 30, 575
PspEI GGTNACC 1 cut(s) 26
PspGI CCWGG 2 cut(s) 30, 575
PspN4I GGNNCC 2 cut(s) 336, 582
PspPI GGNCC 2 cut(s) 170, 209
PspPPI RGGWCCY 1 cut(s) 170
PvuI CGATCG 1 cut(s) 670
RsaI GTAC 2 cut(s) 506, 687
RsaNI GTAC 2 cut(s) 505, 686
RseI CAYNNNNRTG 1 cut(s) 33
SapI GCTCTTC 1 cut(s) 546
SaqAI TTAA 3 cut(s) 105, 699, 795
SatI GCNGC 2 cut(s) 124, 475
Sau3AI GATC 2 cut(s) 213, 667
Sau96I GGNCC 2 cut(s) 170, 209
ScrFI CCNGG 3 cut(s) 6, 32, 577
SduI GDGCHC 3 cut(s) 230, 306, 583
SfaNI GCATC 2 cut(s) 89, 205
SfoI GGCGCC 1 cut(s) 336
SinI GGWCC 1 cut(s) 170
SmiMI CAYNNNNRTG 1 cut(s) 33
Sse9I AATT 4 cut(s) 111, 249, 319, 500
SsiI CCGC 1 cut(s) 131
SspDI GGCGCC 1 cut(s) 334
SspMI CTAG 4 cut(s) 77, 402, 429, 820
StyD4I CCNGG 3 cut(s) 4, 30, 575
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 4 cut(s) 308, 487, 637, 652
TaiI ACGT 1 cut(s) 573
TaqI TCGA 1 cut(s) 670
TasI AATT 4 cut(s) 111, 249, 319, 500
TatI WGTACW 1 cut(s) 504
Tru1I TTAA 3 cut(s) 105, 699, 795
Tru9I TTAA 3 cut(s) 105, 699, 795
TscAI CASTG 4 cut(s) 403, 492, 559, 642
TseFI GTSAC 3 cut(s) 26, 161, 726
TseI GCWGC 2 cut(s) 123, 474
Tsp45I GTSAC 3 cut(s) 26, 161, 726
TspDTI ATGAA 4 cut(s) 32, 78, 300, 357
TspGWI ACGGA 1 cut(s) 645
TspRI CASTG 4 cut(s) 403, 492, 559, 642
Van91I CCANNNNNTGG 1 cut(s) 330
VpaK11BI GGWCC 1 cut(s) 170
XapI RAATTY 1 cut(s) 319
XspI CTAG 4 cut(s) 77, 402, 429, 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.