Rh3AG003400

Dehydrogenase reductase SDR family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0007904
Physical Location & Seq
Reverse (-)
277926 .. 281912
3987 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG003400.1

Sequence Viewer

Length: 984 bp
ATGGTGGTGGTGTTATTCTTGGTTTCTCTGCTTCTTCTTCTCTTGTTTGCTTTAATCAAATTTACCACAGCTGATGGTGATTTCACTTTGATGTCCAAACGCCATGTTAACCGCCACGAAATTGAAGACAAAGTTGTTTGGATAACTGGTGCTAGCCGTGGAATTGGGGAGGTTCTTGCTAAGCAACTTGCAAATTTGGGAGCTAAGCTCATTATTTCCGCCCGTAATGAAGCTGAATTGGAGCGAGTCAAGAAACAACTTACTGGTAAACATGCACCTGATGGTGTGAAAGTTTTGCCATTGGATTTGGCTTCTGGTGAAGATTGTCTCAAGGATGTTGTAGAGAAAGCAGAGTCATTCTTTCCAAATGCTGGTGTTGATTACATGATCCATAATGCTGCTTTTGAGCGTCCTAAAACTACGGCATTGGATGTTACTGAGGAGAGTCTAAAGGTAACTTTCAATGTCAATGTTCTTGGGACAATAACGCTGACCCGGCTACTGGCACCTTACATGCTGAGGCGAGGGAAGGGCCATTTTGTTGTGATGAGCAGTGCTGCAGGAAAGACACCTGCACCAGGTCAGGCCGTATACTCTGCCTCTAAACATGCCTTAAATGGGTACTTTCACACCTTGCGTTCCGAGCTCTATCAGAAAGGAATAGGGGTGACCATTGTTTGTCCTGGTCCAATAGAAACATCAAATGGTACAGGAGCAGCAAGCTCAGAGAATAAAGCTTCTTCTGAGAAGCGTGTGTCATCAGAAAGGTGTGCAGAGTTGACAATAATTGCTGCCACCCATGGTCTAAAGGAAGTTTGGATATCATACCAGCCTGTCCTGGCTGTAATGTACTTGGTGCAGTACATGCCAACCATTGGTTATTGGCTTATGGACAAGATTGGTGGAAATCGAGTTGAAGCTGCTGCAAGGAAGGGCAACACTTACTCATTGAGCTTACTATTTGGGAAAAAGAAGGCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

327

Amino Acids

35.55

Weight (kDa)

9.31

Isoelectric Point (pI)

35.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 44 - 236 1.1e-46 short chain dehydrogenase
KR PF08659 46 - 214 1.1e-14 KR domain
Polysacc_synt_2 PF02719 46 - 236 5.4e-06 Polysaccharide biosynthesis protein
adh_short_C2 PF13561 50 - 264 2.1e-35 Enoyl-(Acyl carrier protein) reductase
NAD_binding_10 PF13460 50 - 262 8.2e-06 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 580
Acc36I ACCTGC 1 cut(s) 580
AccB1I GGYRCC 1 cut(s) 505
AccB7I CCANNNNNTGG 2 cut(s) 371, 875
AccI GTMKAC 1 cut(s) 591
AciI CCGC 2 cut(s) 112, 219
AclWI GGATC 1 cut(s) 382
AcsI RAATTY 2 cut(s) 59, 193
AfaI GTAC 4 cut(s) 623, 709, 851, 863
AfiI CCNNNNNNNGG 5 cut(s) 371, 502, 578, 618, 875
AgsI TTSAA 3 cut(s) 125, 463, 917
AjnI CCWGG 3 cut(s) 577, 682, 837
AluBI AGCT 9 cut(s) 71, 203, 208, 233, 646, 723, 737, 920, 954
AluI AGCT 9 cut(s) 71, 203, 208, 233, 646, 723, 737, 920, 954
Alw21I GWGCWC 1 cut(s) 648
Alw26I GTCTC 1 cut(s) 332
AlwI GGATC 1 cut(s) 382
AoxI GGCC 2 cut(s) 532, 585
ApeKI GCWGC 6 cut(s) 398, 557, 716, 791, 920, 923
ApoI RAATTY 2 cut(s) 59, 193
AspS9I GGNCC 2 cut(s) 532, 686
AsuC2I CCSGG 1 cut(s) 496
AsuHPI GGTGA 3 cut(s) 89, 329, 679
AsuNHI GCTAGC 1 cut(s) 152
AvaII GGWCC 1 cut(s) 686
BanI GGYRCC 1 cut(s) 505
BanII GRGCYC 1 cut(s) 648
BbsI GAAGAC 1 cut(s) 132
Bbv12I GWGCWC 1 cut(s) 648
BbvCI CCTCAGC 1 cut(s) 518
BbvI GCAGC 6 cut(s) 385, 544, 728, 778, 907, 910
BccI CCATC 2 cut(s) 68, 275
BceAI ACGGC 3 cut(s) 141, 438, 572
BciT130I CCWGG 3 cut(s) 579, 684, 839
BcnI CCSGG 1 cut(s) 496
BcoDI GTCTC 1 cut(s) 332
BfaI CTAG 1 cut(s) 153
BfmI CTRYAG 1 cut(s) 558
BfuAI ACCTGC 1 cut(s) 580
BisI GCNGC 6 cut(s) 399, 558, 717, 792, 921, 924
BlpI GCTNAGC 2 cut(s) 180, 204
BlsI GCNGC 6 cut(s) 400, 559, 718, 793, 922, 925
Bme1390I CCNGG 4 cut(s) 496, 579, 684, 839
Bme18I GGWCC 1 cut(s) 686
BmgT120I GGNCC 2 cut(s) 532, 686
BmiI GGNNCC 1 cut(s) 507
BmrFI CCNGG 4 cut(s) 496, 579, 684, 839
BmtI GCTAGC 1 cut(s) 156
BpiI GAAGAC 1 cut(s) 132
BplI GAGNNNNNCTC 2 cut(s) 192, 224
Bpu10I CCTNAGC 1 cut(s) 518
Bpu1102I GCTNAGC 2 cut(s) 180, 204
BpuEI CTTGAG 1 cut(s) 314
BpuMI CCSGG 1 cut(s) 496
BsaJI CCNNGG 2 cut(s) 157, 799
Bsc4I CCNNNNNNNGG 5 cut(s) 371, 502, 578, 618, 875
Bse1I ACTGG 3 cut(s) 151, 268, 507
BseBI CCWGG 3 cut(s) 579, 684, 839
BseDI CCNNGG 2 cut(s) 157, 799
BseGI GGATG 2 cut(s) 340, 436
BseLI CCNNNNNNNGG 5 cut(s) 371, 502, 578, 618, 875
BseMII CTCAG 4 cut(s) 429, 509, 735, 738
BseNI ACTGG 3 cut(s) 151, 268, 507
BseRI GAGGAG 1 cut(s) 455
BseXI GCAGC 6 cut(s) 385, 544, 728, 778, 907, 910
BsgI GTGCAG 3 cut(s) 558, 792, 878
BshFI GGCC 2 cut(s) 534, 587
BshNI GGYRCC 1 cut(s) 505
BsiHKAI GWGCWC 1 cut(s) 648
BsiSI CCGG 1 cut(s) 496
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 5 cut(s) 371, 502, 578, 618, 875
BsmAI GTCTC 1 cut(s) 332
BsmFI GGGAC 1 cut(s) 493
BsnI GGCC 2 cut(s) 534, 587
Bsp1286I GDGCHC 1 cut(s) 648
Bsp143I GATC 1 cut(s) 387
Bsp1720I GCTNAGC 2 cut(s) 180, 204
Bsp19I CCATGG 1 cut(s) 799
BspACI CCGC 2 cut(s) 112, 219
BspANI GGCC 2 cut(s) 534, 587
BspCNI CTCAG 4 cut(s) 430, 510, 736, 737
BspHI TCATGA 1 cut(s) 980
BspLI GGNNCC 1 cut(s) 507
BspMAI CTGCAG 1 cut(s) 562
BspMI ACCTGC 1 cut(s) 580
BspOI GCTAGC 1 cut(s) 156
BspPI GGATC 1 cut(s) 382
BspT107I GGYRCC 1 cut(s) 505
BsrI ACTGG 3 cut(s) 151, 268, 507
BssECI CCNNGG 2 cut(s) 157, 799
BssMI GATC 1 cut(s) 387
BssNAI GTATAC 1 cut(s) 592
BssT1I CCWWGG 1 cut(s) 799
Bst1107I GTATAC 1 cut(s) 592
Bst2UI CCWGG 3 cut(s) 579, 684, 839
BstAPI GCANNNNNTGC 1 cut(s) 865
BstC8I GCNNGC 2 cut(s) 154, 721
BstDEI CTNAG 6 cut(s) 180, 204, 438, 518, 724, 744
BstDSI CCRYGG 2 cut(s) 157, 799
BstEII GGTNACC 1 cut(s) 667
BstENI CCTNNNNNAGG 1 cut(s) 576
BstF5I GGATG 2 cut(s) 340, 436
BstKTI GATC 1 cut(s) 390
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 1 cut(s) 387
BstMWI GCNNNNNNNGC 3 cut(s) 496, 643, 865
BstNI CCWGG 3 cut(s) 579, 684, 839
BstNSI RCATGY 4 cut(s) 275, 517, 611, 868
BstPI GGTNACC 1 cut(s) 667
BstSCI CCNGG 4 cut(s) 494, 577, 682, 837
BstSFI CTRYAG 1 cut(s) 558
BstV1I GCAGC 6 cut(s) 385, 544, 728, 778, 907, 910
BstV2I GAAGAC 1 cut(s) 132
BstZ17I GTATAC 1 cut(s) 592
BsuRI GGCC 2 cut(s) 534, 587
BtgI CCRYGG 2 cut(s) 157, 799
BtsCI GGATG 2 cut(s) 340, 436
BtsI GCAGTG 1 cut(s) 559
BtsIMutI CAGTG 1 cut(s) 559
BveI ACCTGC 1 cut(s) 580
Cac8I GCNNGC 2 cut(s) 154, 721
CciI TCATGA 1 cut(s) 980
Cfr13I GGNCC 2 cut(s) 532, 686
CseI GACGC 1 cut(s) 398
CsiI ACCWGGT 1 cut(s) 577
Csp6I GTAC 4 cut(s) 622, 708, 850, 862
CspCI CAANNNNNGTGG 2 cut(s) 883, 918
CviAII CATG 8 cut(s) 104, 272, 385, 514, 608, 800, 865, 981
CviQI GTAC 4 cut(s) 622, 708, 850, 862
DdeI CTNAG 6 cut(s) 180, 204, 438, 518, 724, 744
DpnI GATC 1 cut(s) 389
DpnII GATC 1 cut(s) 387
EciI GGCGGA 1 cut(s) 208
Ecl136II GAGCTC 1 cut(s) 646
Eco130I CCWWGG 1 cut(s) 799
Eco24I GRGCYC 1 cut(s) 648
Eco32I GATATC 1 cut(s) 822
Eco47I GGWCC 1 cut(s) 686
Eco53kI GAGCTC 1 cut(s) 646
Eco91I GGTNACC 1 cut(s) 667
EcoICRI GAGCTC 1 cut(s) 646
EcoNI CCTNNNNNAGG 1 cut(s) 576
EcoO65I GGTNACC 1 cut(s) 667
EcoRII CCWGG 3 cut(s) 577, 682, 837
EcoRV GATATC 1 cut(s) 822
EcoT14I CCWWGG 1 cut(s) 799
EcoT38I GRGCYC 1 cut(s) 648
ErhI CCWWGG 1 cut(s) 799
FaeI CATG 8 cut(s) 107, 275, 388, 517, 611, 803, 868, 984
FaqI GGGAC 1 cut(s) 493
FatI CATG 8 cut(s) 103, 271, 384, 513, 607, 799, 864, 980
FblI GTMKAC 1 cut(s) 591
Fnu4HI GCNGC 6 cut(s) 399, 558, 717, 792, 921, 924
FokI GGATG 2 cut(s) 347, 443
FriOI GRGCYC 1 cut(s) 648
Fsp4HI GCNGC 6 cut(s) 399, 558, 717, 792, 921, 924
FspBI CTAG 1 cut(s) 153
GluI GCNGC 6 cut(s) 399, 558, 717, 792, 921, 924
HaeIII GGCC 2 cut(s) 534, 587
HapII CCGG 1 cut(s) 496
HgaI GACGC 1 cut(s) 398
Hin1II CATG 8 cut(s) 107, 275, 388, 517, 611, 803, 868, 984
HincII GTYRAC 2 cut(s) 109, 780
HindII GTYRAC 2 cut(s) 109, 780
HindIII AAGCTT 1 cut(s) 735
HinfI GANTC 3 cut(s) 246, 353, 445
HpaI GTTAAC 1 cut(s) 109
HpaII CCGG 1 cut(s) 496
HphI GGTGA 3 cut(s) 89, 329, 679
Hpy166II GTNNAC 4 cut(s) 109, 269, 592, 780
Hpy188I TCNGA 5 cut(s) 643, 654, 727, 745, 763
Hpy188III TCNNGA 2 cut(s) 250, 981
Hpy8I GTNNAC 4 cut(s) 109, 269, 592, 780
HpyAV CCTTC 3 cut(s) 523, 925, 967
HpyCH4V TGCA 7 cut(s) 191, 275, 560, 575, 773, 859, 926
HpyF10VI GCNNNNNNNGC 3 cut(s) 496, 643, 865
HpyF3I CTNAG 6 cut(s) 180, 204, 438, 518, 724, 744
Hsp92II CATG 8 cut(s) 107, 275, 388, 517, 611, 803, 868, 984
KspAI GTTAAC 1 cut(s) 109
Kzo9I GATC 1 cut(s) 387
LmnI GCTCC 3 cut(s) 200, 241, 713
Lsp1109I GCAGC 6 cut(s) 385, 544, 728, 778, 907, 910
MabI ACCWGGT 1 cut(s) 577
MaeI CTAG 1 cut(s) 153
MaeIII GTNAC 3 cut(s) 433, 454, 667
MalI GATC 1 cut(s) 389
MboI GATC 1 cut(s) 387
MboII GAAGA 5 cut(s) 26, 29, 137, 332, 732
MhlI GDGCHC 1 cut(s) 648
MluCI AATT 6 cut(s) 59, 120, 162, 193, 236, 786
MlyI GAGTC 3 cut(s) 255, 362, 454
MnlI CCTC 5 cut(s) 163, 433, 513, 518, 610
MseI TTAA 3 cut(s) 53, 108, 614
MslI CAYNNNNRTG 1 cut(s) 89
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 1 cut(s) 496
MspR9I CCNGG 4 cut(s) 496, 579, 684, 839
MvaI CCWGG 3 cut(s) 579, 684, 839
MwoI GCNNNNNNNGC 3 cut(s) 496, 643, 865
NciI CCSGG 1 cut(s) 496
NcoI CCATGG 1 cut(s) 799
NdeII GATC 1 cut(s) 387
NheI GCTAGC 1 cut(s) 152
NlaIII CATG 8 cut(s) 107, 275, 388, 517, 611, 803, 868, 984
NlaIV GGNNCC 1 cut(s) 507
NmuCI GTSAC 1 cut(s) 667
NspI RCATGY 4 cut(s) 275, 517, 611, 868
PagI TCATGA 1 cut(s) 980
PaqCI CACCTGC 1 cut(s) 580
PflMI CCANNNNNTGG 2 cut(s) 371, 875
PkrI GCNGC 6 cut(s) 400, 559, 718, 793, 922, 925
PleI GAGTC 3 cut(s) 254, 361, 453
PpsI GAGTC 3 cut(s) 254, 361, 453
Psp124BI GAGCTC 1 cut(s) 648
Psp6I CCWGG 3 cut(s) 577, 682, 837
PspEI GGTNACC 1 cut(s) 667
PspGI CCWGG 3 cut(s) 577, 682, 837
PspN4I GGNNCC 1 cut(s) 507
PspPI GGNCC 2 cut(s) 532, 686
PstI CTGCAG 1 cut(s) 562
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 4 cut(s) 623, 709, 851, 863
RsaNI GTAC 4 cut(s) 622, 708, 850, 862
RseI CAYNNNNRTG 1 cut(s) 89
SacI GAGCTC 1 cut(s) 648
SaqAI TTAA 3 cut(s) 53, 108, 614
SatI GCNGC 6 cut(s) 399, 558, 717, 792, 921, 924
Sau3AI GATC 1 cut(s) 387
Sau96I GGNCC 2 cut(s) 532, 686
SchI GAGTC 3 cut(s) 255, 362, 454
ScrFI CCNGG 4 cut(s) 496, 579, 684, 839
SduI GDGCHC 1 cut(s) 648
SexAI ACCWGGT 1 cut(s) 577
SfcI CTRYAG 1 cut(s) 558
SinI GGWCC 1 cut(s) 686
SmiMI CAYNNNNRTG 1 cut(s) 89
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
Sse9I AATT 6 cut(s) 59, 120, 162, 193, 236, 786
SsiI CCGC 2 cut(s) 112, 219
SspMI CTAG 1 cut(s) 153
SstI GAGCTC 1 cut(s) 648
StyD4I CCNGG 4 cut(s) 494, 577, 682, 837
StyI CCWWGG 1 cut(s) 799
TaqI TCGA 1 cut(s) 910
TasI AATT 6 cut(s) 59, 120, 162, 193, 236, 786
TatI WGTACW 2 cut(s) 849, 861
Tru1I TTAA 3 cut(s) 53, 108, 614
Tru9I TTAA 3 cut(s) 53, 108, 614
TscAI CASTG 1 cut(s) 559
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 6 cut(s) 398, 557, 716, 791, 920, 923
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 1 cut(s) 243
TspRI CASTG 1 cut(s) 559
Van91I CCANNNNNTGG 2 cut(s) 371, 875
VpaK11BI GGWCC 1 cut(s) 686
XagI CCTNNNNNAGG 1 cut(s) 576
XapI RAATTY 2 cut(s) 59, 193
XceI RCATGY 4 cut(s) 275, 517, 611, 868
XmiI GTMKAC 1 cut(s) 591
XspI CTAG 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.