MD04G1247200.v1.1

Dehydrogenase reductase SDR family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007904
Physical Location & Seq
Forward (+)
32193318 .. 32197671
4354 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1247200.v1.1.491

Sequence Viewer

Length: 990 bp
ATGGCGATGGTGGTGGCGGTGATAGTCTTCGTTTCTCTGTTTCTGTTTGTTTTTTTAGCAATCAAATTTGCAACAGCTGATGGGGATTTTACTTTGACGTCCAAAGGCGGTGCGAAGCGCGACCAGATTGAAGATAAAGTTGTTTGGATAACTGGCGCTAGCCGCGGAATTGGGGAGATTCTTGCTAAGCAACTTGCGAAATTGGGGGCTAAGCTCATTATTTCTGCACGTAATGAAGCTGAATTGGAGCGTGTCAAGAGAGAACTGACTGGTAAACATGTACCTGATGGTGTAAAGGTTTTGCCATTGGATTTGGCATCTGGTGAAGCTCATCTCAAGGATGCTGTAGAGAAAGCAGAGTCCTTCTTTCTTAATGCTGGTGTTGATTATATGATCCACAATGCAGCTGTTGAGCGTCCTAAAACTTCAGCTTTGGATGTTACTGAGGAGAGTCTGAAGGAGACATTCAATGTCAATGTTCTTGGGACACTAACGCTTACTCGGTTGCTAGCACCTTACATGCTGAGGCGGGGGAAGGGACATTTTGTTGTGATGAGCAGTGCTGCGGGAAAGACACCAGCGCCAGGTCAGGCCATGTACTCTGCCTCTAAACATGCATTAAACGGGTACTTTCACACATTGCGTTCTGAGCTGTGTCAGAAAGGAATCCAGGTGACCATTGTTTGCCCTGGGCCAATAGAAACATCAAATGCTTCTGGAGCAGCAAGCTCAGAAAATAAAGCTTCTCGTGAGAAGCGTGTGTCGTCAGAAAGATGTGCGGAGTTGACGATAGTTGCTGCCACCCATGGTCTGAAAGAAGCTTGGATATCTTACCAGCCTGTGCTGGCTGTAATGTACTTGGTGCAATACATGCCAACTATTGGTTATTGGCTCATGGACAAGATTGGTGGAAATCGAGTTGAAGCAGCTGCACGAAAGGGGAACACTTACTCATTGAGCTTACTGTTTGGGAAAAAGAAGGCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

330

Amino Acids

35.58

Weight (kDa)

9.39

Isoelectric Point (pI)

32.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 46 - 240 6.8e-48 short chain dehydrogenase
KR PF08659 48 - 217 1.4e-15 KR domain
Polysacc_synt_2 PF02719 48 - 237 7e-07 Polysaccharide biosynthesis protein
Epimerase PF01370 49 - 216 1.9e-06 NAD dependent epimerase/dehydratase family
adh_short_C2 PF13561 52 - 266 2e-36 Enoyl-(Acyl carrier protein) reductase
NAD_binding_10 PF13460 52 - 264 8.9e-07 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 101
AccB7I CCANNNNNTGG 1 cut(s) 881
AccII CGCG 2 cut(s) 120, 165
AciI CCGC 7 cut(s) 17, 108, 163, 165, 529, 566, 779
AclWI GGATC 1 cut(s) 388
AcsI RAATTY 1 cut(s) 65
AcuI CTGAAG 2 cut(s) 411, 476
AcyI GRCGYC 1 cut(s) 98
AfaI GTAC 4 cut(s) 282, 599, 629, 857
AfiI CCNNNNNNNGG 2 cut(s) 584, 881
AflIII ACRYGT 1 cut(s) 277
AgsI TTSAA 3 cut(s) 131, 469, 923
AjnI CCWGG 3 cut(s) 583, 669, 688
Alw26I GTCTC 1 cut(s) 455
AlwI GGATC 1 cut(s) 388
AoxI GGCC 2 cut(s) 591, 692
ApeKI GCWGC 6 cut(s) 404, 563, 722, 797, 926, 929
ApoI RAATTY 1 cut(s) 65
AspLEI GCGC 3 cut(s) 120, 158, 583
AspS9I GGNCC 1 cut(s) 692
AsuHPI GGTGA 3 cut(s) 31, 335, 685
AsuNHI GCTAGC 2 cut(s) 158, 508
BauI CACGAG 1 cut(s) 747
BbsI GAAGAC 1 cut(s) 19
BbvCI CCTCAGC 1 cut(s) 524
BbvI GCAGC 6 cut(s) 416, 550, 734, 784, 916, 938
BccI CCATC 2 cut(s) 74, 281
BciT130I CCWGG 3 cut(s) 585, 671, 690
BcoDI GTCTC 1 cut(s) 455
BfaI CTAG 2 cut(s) 159, 509
BfmI CTRYAG 1 cut(s) 345
BfoI RGCGCY 2 cut(s) 159, 584
BisI GCNGC 7 cut(s) 163, 405, 564, 723, 798, 927, 930
BlpI GCTNAGC 2 cut(s) 186, 210
BlsI GCNGC 7 cut(s) 164, 406, 565, 724, 799, 928, 931
Bme1390I CCNGG 3 cut(s) 585, 671, 690
BmgT120I GGNCC 1 cut(s) 692
BmrFI CCNGG 3 cut(s) 585, 671, 690
BmsI GCATC 2 cut(s) 326, 331
BmtI GCTAGC 2 cut(s) 162, 512
BpiI GAAGAC 1 cut(s) 19
BpmI CTGGAG 1 cut(s) 738
Bpu10I CCTNAGC 1 cut(s) 524
Bpu1102I GCTNAGC 2 cut(s) 186, 210
BpuEI CTTGAG 1 cut(s) 320
BsaAI YACGTR 1 cut(s) 230
BsaHI GRCGYC 1 cut(s) 98
BsaJI CCNNGG 4 cut(s) 163, 688, 689, 805
Bsc4I CCNNNNNNNGG 2 cut(s) 584, 881
Bse1I ACTGG 2 cut(s) 157, 274
Bse3DI GCAATG 1 cut(s) 638
BseBI CCWGG 3 cut(s) 585, 671, 690
BseDI CCNNGG 4 cut(s) 163, 688, 689, 805
BseGI GGATG 2 cut(s) 346, 442
BseLI CCNNNNNNNGG 2 cut(s) 584, 881
BseMI GCAATG 1 cut(s) 638
BseMII CTCAG 4 cut(s) 435, 515, 639, 744
BseNI ACTGG 2 cut(s) 157, 274
BseRI GAGGAG 1 cut(s) 461
BseXI GCAGC 6 cut(s) 416, 550, 734, 784, 916, 938
BsgI GTGCAG 2 cut(s) 210, 915
Bsh1236I CGCG 2 cut(s) 120, 165
BshFI GGCC 2 cut(s) 593, 694
BslFI GGGAC 2 cut(s) 499, 552
BslI CCNNNNNNNGG 2 cut(s) 584, 881
BsmAI GTCTC 1 cut(s) 455
BsmFI GGGAC 2 cut(s) 499, 552
BsnI GGCC 2 cut(s) 593, 694
Bsp143I GATC 1 cut(s) 393
Bsp1720I GCTNAGC 2 cut(s) 186, 210
Bsp19I CCATGG 1 cut(s) 805
BspACI CCGC 7 cut(s) 17, 108, 163, 165, 529, 566, 779
BspANI GGCC 2 cut(s) 593, 694
BspCNI CTCAG 4 cut(s) 436, 516, 640, 743
BspFNI CGCG 2 cut(s) 120, 165
BspHI TCATGA 1 cut(s) 986
BspOI GCTAGC 2 cut(s) 162, 512
BspPI GGATC 1 cut(s) 388
BsrDI GCAATG 1 cut(s) 638
BsrI ACTGG 2 cut(s) 157, 274
BssECI CCNNGG 4 cut(s) 163, 688, 689, 805
BssMI GATC 1 cut(s) 393
BssNI GRCGYC 1 cut(s) 98
BssSI CACGAG 1 cut(s) 747
BssT1I CCWWGG 1 cut(s) 805
Bst2BI CACGAG 1 cut(s) 747
Bst2UI CCWGG 3 cut(s) 585, 671, 690
Bst4CI ACNGT 1 cut(s) 966
BstACI GRCGYC 1 cut(s) 98
BstAPI GCANNNNNTGC 1 cut(s) 871
BstBAI YACGTR 1 cut(s) 230
BstC8I GCNNGC 4 cut(s) 160, 510, 727, 846
BstDEI CTNAG 6 cut(s) 186, 210, 444, 524, 648, 730
BstDSI CCRYGG 2 cut(s) 163, 805
BstEII GGTNACC 1 cut(s) 673
BstF5I GGATG 2 cut(s) 346, 442
BstFNI CGCG 2 cut(s) 120, 165
BstH2I RGCGCY 2 cut(s) 159, 584
BstHHI GCGC 3 cut(s) 120, 158, 583
BstKTI GATC 1 cut(s) 396
BstMAI GTCTC 1 cut(s) 455
BstMBI GATC 1 cut(s) 393
BstMWI GCNNNNNNNGC 4 cut(s) 162, 649, 719, 871
BstNI CCWGG 3 cut(s) 585, 671, 690
BstNSI RCATGY 4 cut(s) 281, 523, 617, 874
BstPI GGTNACC 1 cut(s) 673
BstSCI CCNGG 3 cut(s) 583, 669, 688
BstSFI CTRYAG 1 cut(s) 345
BstUI CGCG 2 cut(s) 120, 165
BstV1I GCAGC 6 cut(s) 416, 550, 734, 784, 916, 938
BstV2I GAAGAC 1 cut(s) 19
BsuRI GGCC 2 cut(s) 593, 694
BtgI CCRYGG 2 cut(s) 163, 805
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 2 cut(s) 346, 442
BtsI GCAGTG 1 cut(s) 565
BtsIMutI CAGTG 1 cut(s) 565
Cac8I GCNNGC 4 cut(s) 160, 510, 727, 846
CciI TCATGA 1 cut(s) 986
CfoI GCGC 3 cut(s) 120, 158, 583
Cfr13I GGNCC 1 cut(s) 692
Cfr42I CCGCGG 1 cut(s) 166
CseI GACGC 1 cut(s) 404
Csp6I GTAC 4 cut(s) 281, 598, 628, 856
CspCI CAANNNNNGTGG 2 cut(s) 889, 924
CviAII CATG 8 cut(s) 278, 520, 595, 614, 806, 871, 895, 987
CviQI GTAC 4 cut(s) 281, 598, 628, 856
DdeI CTNAG 6 cut(s) 186, 210, 444, 524, 648, 730
DpnI GATC 1 cut(s) 395
DpnII GATC 1 cut(s) 393
Eco130I CCWWGG 1 cut(s) 805
Eco32I GATATC 1 cut(s) 828
Eco57I CTGAAG 2 cut(s) 411, 476
Eco91I GGTNACC 1 cut(s) 673
EcoO65I GGTNACC 1 cut(s) 673
EcoRII CCWGG 3 cut(s) 583, 669, 688
EcoRV GATATC 1 cut(s) 828
EcoT14I CCWWGG 1 cut(s) 805
EcoT22I ATGCAT 1 cut(s) 619
ErhI CCWWGG 1 cut(s) 805
FaeI CATG 8 cut(s) 281, 523, 598, 617, 809, 874, 898, 990
FaqI GGGAC 2 cut(s) 499, 552
FatI CATG 8 cut(s) 277, 519, 594, 613, 805, 870, 894, 986
FauI CCCGC 2 cut(s) 522, 559
Fnu4HI GCNGC 7 cut(s) 163, 405, 564, 723, 798, 927, 930
FokI GGATG 2 cut(s) 353, 449
Fsp4HI GCNGC 7 cut(s) 163, 405, 564, 723, 798, 927, 930
FspBI CTAG 2 cut(s) 159, 509
GlaI GCGC 3 cut(s) 119, 157, 582
GluI GCNGC 7 cut(s) 163, 405, 564, 723, 798, 927, 930
GsuI CTGGAG 1 cut(s) 738
HaeII RGCGCY 2 cut(s) 159, 584
HaeIII GGCC 2 cut(s) 593, 694
HgaI GACGC 1 cut(s) 404
HhaI GCGC 3 cut(s) 120, 158, 583
Hin1I GRCGYC 1 cut(s) 98
Hin1II CATG 8 cut(s) 281, 523, 598, 617, 809, 874, 898, 990
Hin6I GCGC 3 cut(s) 118, 156, 581
HinP1I GCGC 3 cut(s) 118, 156, 581
HincII GTYRAC 1 cut(s) 786
HindII GTYRAC 1 cut(s) 786
HindIII AAGCTT 2 cut(s) 741, 819
HinfI GANTC 4 cut(s) 178, 359, 451, 666
HphI GGTGA 3 cut(s) 31, 335, 685
Hpy166II GTNNAC 2 cut(s) 275, 786
Hpy188I TCNGA 6 cut(s) 456, 649, 660, 733, 769, 813
Hpy188III TCNNGA 4 cut(s) 256, 717, 749, 987
Hpy8I GTNNAC 2 cut(s) 275, 786
HpyAV CCTTC 4 cut(s) 373, 451, 529, 973
HpyCH4III ACNGT 1 cut(s) 966
HpyCH4IV ACGT 2 cut(s) 98, 229
HpyCH4V TGCA 6 cut(s) 71, 227, 404, 617, 865, 932
HpyF10VI GCNNNNNNNGC 4 cut(s) 162, 649, 719, 871
HpyF3I CTNAG 6 cut(s) 186, 210, 444, 524, 648, 730
HpySE526I ACGT 2 cut(s) 98, 229
Hsp92I GRCGYC 1 cut(s) 98
Hsp92II CATG 8 cut(s) 281, 523, 598, 617, 809, 874, 898, 990
HspAI GCGC 3 cut(s) 118, 156, 581
KspI CCGCGG 1 cut(s) 166
Kzo9I GATC 1 cut(s) 393
LmnI GCTCC 2 cut(s) 247, 719
Lsp1109I GCAGC 6 cut(s) 416, 550, 734, 784, 916, 938
LweI GCATC 2 cut(s) 326, 331
MaeI CTAG 2 cut(s) 159, 509
MaeII ACGT 2 cut(s) 98, 229
MaeIII GTNAC 2 cut(s) 439, 673
MalI GATC 1 cut(s) 395
MboI GATC 1 cut(s) 393
MboII GAAGA 2 cut(s) 19, 143
MluCI AATT 4 cut(s) 65, 168, 200, 242
MlyI GAGTC 2 cut(s) 368, 460
MnlI CCTC 3 cut(s) 439, 519, 616
Mph1103I ATGCAT 1 cut(s) 619
MseI TTAA 2 cut(s) 372, 620
MspA1I CMGCKG 4 cut(s) 77, 165, 407, 929
MspR9I CCNGG 3 cut(s) 585, 671, 690
MvaI CCWGG 3 cut(s) 585, 671, 690
MvnI CGCG 2 cut(s) 120, 165
MwoI GCNNNNNNNGC 4 cut(s) 162, 649, 719, 871
NcoI CCATGG 1 cut(s) 805
NdeII GATC 1 cut(s) 393
NheI GCTAGC 2 cut(s) 158, 508
NlaIII CATG 8 cut(s) 281, 523, 598, 617, 809, 874, 898, 990
NmuCI GTSAC 1 cut(s) 673
NsiI ATGCAT 1 cut(s) 619
NspI RCATGY 4 cut(s) 281, 523, 617, 874
PagI TCATGA 1 cut(s) 986
PasI CCCWGGG 1 cut(s) 689
PciI ACATGT 1 cut(s) 277
PcsI WCGNNNNNNNCGW 1 cut(s) 754
PfeI GAWTC 2 cut(s) 178, 666
PflMI CCANNNNNTGG 1 cut(s) 881
PkrI GCNGC 7 cut(s) 164, 406, 565, 724, 799, 928, 931
PleI GAGTC 2 cut(s) 367, 459
PpsI GAGTC 2 cut(s) 367, 459
Ppu21I YACGTR 1 cut(s) 230
PscI ACATGT 1 cut(s) 277
Psp6I CCWGG 3 cut(s) 583, 669, 688
PspEI GGTNACC 1 cut(s) 673
PspGI CCWGG 3 cut(s) 583, 669, 688
PspPI GGNCC 1 cut(s) 692
PvuII CAGCTG 3 cut(s) 77, 407, 929
RsaI GTAC 4 cut(s) 282, 599, 629, 857
RsaNI GTAC 4 cut(s) 281, 598, 628, 856
SacII CCGCGG 1 cut(s) 166
SaqAI TTAA 2 cut(s) 372, 620
SatI GCNGC 7 cut(s) 163, 405, 564, 723, 798, 927, 930
Sau3AI GATC 1 cut(s) 393
Sau96I GGNCC 1 cut(s) 692
SchI GAGTC 2 cut(s) 368, 460
ScrFI CCNGG 3 cut(s) 585, 671, 690
SfaNI GCATC 2 cut(s) 326, 331
SfcI CTRYAG 1 cut(s) 345
Sfr303I CCGCGG 1 cut(s) 166
SgrBI CCGCGG 1 cut(s) 166
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
Sse9I AATT 4 cut(s) 65, 168, 200, 242
SsiI CCGC 7 cut(s) 17, 108, 163, 165, 529, 566, 779
SspMI CTAG 2 cut(s) 159, 509
StyD4I CCNGG 3 cut(s) 583, 669, 688
StyI CCWWGG 1 cut(s) 805
TaaI ACNGT 1 cut(s) 966
TaiI ACGT 2 cut(s) 101, 232
TaqI TCGA 1 cut(s) 916
TasI AATT 4 cut(s) 65, 168, 200, 242
TatI WGTACW 2 cut(s) 597, 855
TauI GCSGC 1 cut(s) 165
TfiI GAWTC 2 cut(s) 178, 666
Tru1I TTAA 2 cut(s) 372, 620
Tru9I TTAA 2 cut(s) 372, 620
TscAI CASTG 1 cut(s) 565
TseFI GTSAC 1 cut(s) 673
TseI GCWGC 6 cut(s) 404, 563, 722, 797, 926, 929
Tsp45I GTSAC 1 cut(s) 673
TspDTI ATGAA 1 cut(s) 249
TspRI CASTG 1 cut(s) 565
Van91I CCANNNNNTGG 1 cut(s) 881
XapI RAATTY 1 cut(s) 65
XceI RCATGY 4 cut(s) 281, 523, 617, 874
XspI CTAG 2 cut(s) 159, 509
ZraI GACGTC 1 cut(s) 99
Zsp2I ATGCAT 1 cut(s) 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.