AT3G03500

Deoxyribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006626
Physical Location & Seq
Forward (+)
832718 .. 834600
1883 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G03500.2

Sequence Viewer

Length: 819 bp
ATGAAGTTGTTTGATGCTCATTGTCACCTCCAAGACAAGAGGGTCATCGACAAAGCCTCGCAGCTCATATCTGCTGCTTTAGCTGTCGGTGTTACCAATTTCGCAGTCAATGGAACCTCCGAGAAAGATTGGAACTTGGTGAAAGAGATGGGAGAGACGTATCCTTCTGTTGTTCCTTGCTTTGGACTCCATCCTTGGTTTATTGCTGATAGAAGCCCTCATTGGTTTAAAACATTGAAGAAGTTCTTTGAGACCACTCCTACTGCTGCTGTTGGAGAAATTGGTTTAGACAAAGGTCCCTTAGCAGGAGGAATTGATTACTCAGACCAGCTAGTCGTGTTTCGCCCACAGCTTGAACTTGCAAAAGAACTGAACAAACCAGTAGCAGTTCATTGCATCGATGCATTTGATGATCTCCTAGAGATAATGAGATCCATAGGGCCTTTTCCTGCTGGGGTGATTCTTCATTCATTCAATGGTTCAGCTGAAGTTGTTCCTAAACTCGCTGAGCTTGGTGCATACTTCTCTTTTTCTGGCTGGTTCACTTACATTGATGAGAAAATTGCCAAGAAGACTTTGAAATCGATTCCTTCCGATAGGCTCTTATTGGAGACGGATTCACCTGACGGGTTACCAAAATCAGATGAGAGTTCTTCGGATCCAAAACCAACTCTCAATGAGCCTGCAAACATTCTTGCTGTACTAGATTATGTTGCGAACCTGTCAAACATGAAGAAGGAAGAGCTTGCGGAGTTAAGTTATGTGAATTCTGTGAGGTTGTTTTCTTATCCAGGTTCCAAATTGCTTACAGACCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TatD_DNase

Protein Analysis

272

Amino Acids

29.98

Weight (kDa)

5.2

Isoelectric Point (pI)

32.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TatD_DNase PF01026 36 - 228 3.5e-49 TatD related DNase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 41, 332
AciI CCGC 1 cut(s) 749
AclWI GGATC 3 cut(s) 426, 653, 666
AcsI RAATTY 1 cut(s) 766
AcuI CTGAAG 1 cut(s) 507
AfaI GTAC 1 cut(s) 702
AfiI CCNNNNNNNGG 2 cut(s) 182, 305
AgsI TTSAA 4 cut(s) 238, 356, 475, 580
AjnI CCWGG 1 cut(s) 790
AjuI GAANNNNNNNTTGG 2 cut(s) 205, 237
AluBI AGCT 7 cut(s) 64, 83, 331, 352, 485, 511, 745
AluI AGCT 7 cut(s) 64, 83, 331, 352, 485, 511, 745
Alw26I GTCTC 3 cut(s) 149, 245, 605
AlwI GGATC 3 cut(s) 426, 653, 666
AoxI GGCC 1 cut(s) 440
ApeKI GCWGC 3 cut(s) 61, 74, 266
ApoI RAATTY 1 cut(s) 766
Asp700I GAANNNNTTC 2 cut(s) 242, 492
AspS9I GGNCC 2 cut(s) 296, 440
AsuHPI GGTGA 4 cut(s) 17, 151, 469, 612
AvaII GGWCC 1 cut(s) 296
BamHI GGATCC 1 cut(s) 658
BbsI GAAGAC 1 cut(s) 578
BbvI GCAGC 3 cut(s) 61, 73, 253
BccI CCATC 2 cut(s) 142, 198
BciT130I CCWGG 1 cut(s) 792
BciVI GTATCC 1 cut(s) 171
BcoDI GTCTC 3 cut(s) 149, 245, 605
BfaI CTAG 3 cut(s) 332, 419, 704
BfuI GTATCC 1 cut(s) 171
BisI GCNGC 3 cut(s) 62, 75, 267
BlpI GCTNAGC 1 cut(s) 507
BlsI GCNGC 3 cut(s) 63, 76, 268
Bme1390I CCNGG 1 cut(s) 792
Bme18I GGWCC 1 cut(s) 296
BmgT120I GGNCC 2 cut(s) 296, 440
BmiI GGNNCC 4 cut(s) 115, 298, 660, 796
BmrFI CCNGG 1 cut(s) 792
BmsI GCATC 3 cut(s) 4, 391, 405
BoxI GACNNNNGTC 1 cut(s) 294
BpiI GAAGAC 1 cut(s) 578
Bpu10I CCTNAGC 1 cut(s) 301
Bpu1102I GCTNAGC 1 cut(s) 507
Bsa29I ATCGAT 2 cut(s) 399, 584
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 1 cut(s) 194
Bsc4I CCNNNNNNNGG 2 cut(s) 182, 305
Bse1I ACTGG 2 cut(s) 380, 814
Bse3DI GCAATG 1 cut(s) 391
BseBI CCWGG 1 cut(s) 792
BseCI ATCGAT 2 cut(s) 399, 584
BseDI CCNNGG 1 cut(s) 194
BseGI GGATG 1 cut(s) 190
BseLI CCNNNNNNNGG 2 cut(s) 182, 305
BseMI GCAATG 1 cut(s) 391
BseMII CTCAG 2 cut(s) 336, 498
BseNI ACTGG 2 cut(s) 380, 814
BseXI GCAGC 3 cut(s) 61, 73, 253
BseYI CCCAGC 1 cut(s) 452
BshFI GGCC 1 cut(s) 442
BshVI ATCGAT 2 cut(s) 399, 584
BslFI GGGAC 1 cut(s) 282
BslI CCNNNNNNNGG 2 cut(s) 182, 305
BsmAI GTCTC 3 cut(s) 149, 245, 605
BsmBI CGTCTC 2 cut(s) 149, 605
BsmFI GGGAC 1 cut(s) 282
BsnI GGCC 1 cut(s) 442
Bso31I GGTCTC 1 cut(s) 245
Bsp143I GATC 3 cut(s) 412, 431, 658
Bsp1720I GCTNAGC 1 cut(s) 507
BspACI CCGC 1 cut(s) 749
BspANI GGCC 1 cut(s) 442
BspCNI CTCAG 2 cut(s) 335, 499
BspDI ATCGAT 2 cut(s) 399, 584
BspLI GGNNCC 4 cut(s) 115, 298, 660, 796
BspPI GGATC 3 cut(s) 426, 653, 666
BspQI GCTCTTC 1 cut(s) 735
BspTNI GGTCTC 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 391
BsrI ACTGG 2 cut(s) 380, 814
BssECI CCNNGG 1 cut(s) 194
BssMI GATC 3 cut(s) 412, 431, 658
BssT1I CCWWGG 1 cut(s) 194
Bst2UI CCWGG 1 cut(s) 792
Bst6I CTCTTC 1 cut(s) 735
BstC8I GCNNGC 2 cut(s) 684, 747
BstDEI CTNAG 3 cut(s) 301, 322, 507
BstEII GGTNACC 1 cut(s) 630
BstF5I GGATG 1 cut(s) 190
BstKTI GATC 3 cut(s) 415, 434, 661
BstMAI GTCTC 3 cut(s) 149, 245, 605
BstMBI GATC 3 cut(s) 412, 431, 658
BstMWI GCNNNNNNNGC 1 cut(s) 80
BstNI CCWGG 1 cut(s) 792
BstPAI GACNNNNGTC 1 cut(s) 294
BstPI GGTNACC 1 cut(s) 630
BstSCI CCNGG 1 cut(s) 790
BstV1I GCAGC 3 cut(s) 61, 73, 253
BstV2I GAAGAC 1 cut(s) 578
BstX2I RGATCY 2 cut(s) 431, 658
BstYI RGATCY 2 cut(s) 431, 658
Bsu15I ATCGAT 2 cut(s) 399, 584
BsuI GTATCC 1 cut(s) 171
BsuRI GGCC 1 cut(s) 442
BsuTUI ATCGAT 2 cut(s) 399, 584
BtsCI GGATG 1 cut(s) 190
Cac8I GCNNGC 2 cut(s) 684, 747
Cfr13I GGNCC 2 cut(s) 296, 440
ClaI ATCGAT 2 cut(s) 399, 584
Csp6I GTAC 1 cut(s) 701
CviAII CATG 1 cut(s) 730
CviQI GTAC 1 cut(s) 701
DdeI CTNAG 3 cut(s) 301, 322, 507
DpnI GATC 3 cut(s) 414, 433, 660
DpnII GATC 3 cut(s) 412, 431, 658
DraI TTTAAA 1 cut(s) 229
DrdI GACNNNNNNGTC 2 cut(s) 41, 332
DseDI GACNNNNNNGTC 2 cut(s) 41, 332
Eam1104I CTCTTC 1 cut(s) 735
EarI CTCTTC 1 cut(s) 735
Eco130I CCWWGG 1 cut(s) 194
Eco31I GGTCTC 1 cut(s) 245
Eco47I GGWCC 1 cut(s) 296
Eco57I CTGAAG 1 cut(s) 507
Eco91I GGTNACC 1 cut(s) 630
EcoO109I RGGNCCY 2 cut(s) 296, 440
EcoO65I GGTNACC 1 cut(s) 630
EcoRI GAATTC 1 cut(s) 766
EcoRII CCWGG 1 cut(s) 790
EcoT14I CCWWGG 1 cut(s) 194
EcoT22I ATGCAT 1 cut(s) 406
ErhI CCWWGG 1 cut(s) 194
Esp3I CGTCTC 2 cut(s) 149, 605
FaeI CATG 1 cut(s) 733
FaiI YATR 6 cut(s) 68, 437, 520, 711, 731, 762
FalI AAGNNNNNCTT 2 cut(s) 230, 262
FaqI GGGAC 1 cut(s) 282
FatI CATG 1 cut(s) 729
Fnu4HI GCNGC 3 cut(s) 62, 75, 267
FokI GGATG 1 cut(s) 177
Fsp4HI GCNGC 3 cut(s) 62, 75, 267
FspBI CTAG 3 cut(s) 332, 419, 704
GluI GCNGC 3 cut(s) 62, 75, 267
GsaI CCCAGC 1 cut(s) 456
HaeIII GGCC 1 cut(s) 442
Hin1II CATG 1 cut(s) 733
HinfI GANTC 4 cut(s) 186, 460, 586, 617
HphI GGTGA 4 cut(s) 17, 151, 469, 612
Hpy166II GTNNAC 1 cut(s) 543
Hpy188I TCNGA 5 cut(s) 121, 325, 595, 643, 658
Hpy8I GTNNAC 1 cut(s) 543
HpyAV CCTTC 3 cut(s) 174, 600, 730
HpyCH4IV ACGT 1 cut(s) 158
HpyCH4V TGCA 5 cut(s) 362, 396, 404, 518, 686
HpyF10VI GCNNNNNNNGC 1 cut(s) 80
HpyF3I CTNAG 3 cut(s) 301, 322, 507
HpySE526I ACGT 1 cut(s) 158
Hsp92II CATG 1 cut(s) 733
Kzo9I GATC 3 cut(s) 412, 431, 658
LguI GCTCTTC 1 cut(s) 735
Lsp1109I GCAGC 3 cut(s) 61, 73, 253
LweI GCATC 3 cut(s) 4, 391, 405
MaeI CTAG 3 cut(s) 332, 419, 704
MaeII ACGT 1 cut(s) 158
MaeIII GTNAC 3 cut(s) 23, 91, 630
MalI GATC 3 cut(s) 414, 433, 660
MboI GATC 3 cut(s) 412, 431, 658
MboII GAAGA 6 cut(s) 250, 455, 583, 645, 745, 752
MflI RGATCY 2 cut(s) 431, 658
MluCI AATT 6 cut(s) 97, 279, 312, 561, 766, 800
MlyI GAGTC 1 cut(s) 180
MmeI TCCRAC 1 cut(s) 253
MnlI CCTC 7 cut(s) 33, 38, 67, 127, 228, 302, 768
Mph1103I ATGCAT 1 cut(s) 406
MroXI GAANNNNTTC 2 cut(s) 242, 492
MseI TTAA 2 cut(s) 228, 755
MspA1I CMGCKG 1 cut(s) 485
MspR9I CCNGG 1 cut(s) 792
MvaI CCWGG 1 cut(s) 792
MwoI GCNNNNNNNGC 1 cut(s) 80
NdeII GATC 3 cut(s) 412, 431, 658
NlaIII CATG 1 cut(s) 733
NlaIV GGNNCC 4 cut(s) 115, 298, 660, 796
NmuCI GTSAC 1 cut(s) 23
NsiI ATGCAT 1 cut(s) 406
PciSI GCTCTTC 1 cut(s) 735
PdmI GAANNNNTTC 2 cut(s) 242, 492
PfeI GAWTC 3 cut(s) 460, 586, 617
PkrI GCNGC 3 cut(s) 63, 76, 268
PleI GAGTC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 180
PpuMI RGGWCCY 1 cut(s) 296
PshAI GACNNNNGTC 1 cut(s) 294
Psp5II RGGWCCY 1 cut(s) 296
Psp6I CCWGG 1 cut(s) 790
PspEI GGTNACC 1 cut(s) 630
PspFI CCCAGC 1 cut(s) 452
PspGI CCWGG 1 cut(s) 790
PspN4I GGNNCC 4 cut(s) 115, 298, 660, 796
PspPI GGNCC 2 cut(s) 296, 440
PspPPI RGGWCCY 1 cut(s) 296
PsuI RGATCY 2 cut(s) 431, 658
PvuII CAGCTG 1 cut(s) 485
RsaI GTAC 1 cut(s) 702
RsaNI GTAC 1 cut(s) 701
SapI GCTCTTC 1 cut(s) 735
SaqAI TTAA 2 cut(s) 228, 755
SatI GCNGC 3 cut(s) 62, 75, 267
Sau3AI GATC 3 cut(s) 412, 431, 658
Sau96I GGNCC 2 cut(s) 296, 440
SchI GAGTC 1 cut(s) 180
ScrFI CCNGG 1 cut(s) 792
SfaNI GCATC 3 cut(s) 4, 391, 405
SinI GGWCC 1 cut(s) 296
Sse9I AATT 6 cut(s) 97, 279, 312, 561, 766, 800
SsiI CCGC 1 cut(s) 749
SspMI CTAG 3 cut(s) 332, 419, 704
StyD4I CCNGG 1 cut(s) 790
StyI CCWWGG 1 cut(s) 194
TaiI ACGT 1 cut(s) 161
TaqI TCGA 3 cut(s) 48, 399, 584
TasI AATT 6 cut(s) 97, 279, 312, 561, 766, 800
TatI WGTACW 1 cut(s) 700
TfiI GAWTC 3 cut(s) 460, 586, 617
Tru1I TTAA 2 cut(s) 228, 755
Tru9I TTAA 2 cut(s) 228, 755
TseFI GTSAC 1 cut(s) 23
TseI GCWGC 3 cut(s) 61, 74, 266
Tsp45I GTSAC 1 cut(s) 23
TspDTI ATGAA 5 cut(s) 17, 380, 455, 459, 746
TspGWI ACGGA 1 cut(s) 629
VpaK11BI GGWCC 1 cut(s) 296
XapI RAATTY 1 cut(s) 766
XmnI GAANNNNTTC 2 cut(s) 242, 492
XspI CTAG 3 cut(s) 332, 419, 704
Zsp2I ATGCAT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.