MD15G1141100.v1.1

Deoxyribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006626
Physical Location & Seq
Forward (+)
10412789 .. 10415984
3196 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1141100.v1.1.491

Sequence Viewer

Length: 900 bp
ATGATGAAATTGTTTGACGCGCACTGTCACCTACAAGACCCAAGAATCTTCGACAAGGCGCCCCAATTGATTGCGGCAACCCTCGATTCTGGCGTCGTTCGTTTTGCCGTCAATGGAGTCTCTGAGAAAGATTGGCACTTGGTCAAGCAGCTTGGGGAGTCCTATCCTTGCGTAATTCCATGCTTCGGTCTCCATCCTTGGTTTGTTGCAGAGAGGACCCCGAATTGGCTTAACACTTTAAAACAATTCTTCGAGTCTACGCCCTCTGCAGCAGTTGGAGAGATTGGCTTGGACAAAGGTTCGCATGGGAAGAAGGTCGATTTCACGGATCAGGTTAATGTATTCAGGCAACAACTTGAACTTGCAAAAGAGTTAAAAAGGCCAGCATCCATCCATTGTGTCTGTGCTTTTGGTGACCTTCTTCAGATAATGAAATCTTTAGGGCCTTTTCCTGCTGGCGTGATTCTTCATTCTTACCTAGGTTCTGCTGAGATGGTTCCTGAATTTGCCAGTCTTGGTGCTTACTTTTCGTTCTCTGGATTTCTCATGTCCATGAAAGTAAACAAGGCAAAGAGGATGCTGAAGATGGTACCTTCTGAAAGAATATTACTGGAGACAGATGCACCTGATGCATTACCAAAGTCTGAGTTAGGTTCTCTACATCTGGTTGAAGGAGATGCTTCCTTCCCTAAAGAGCTCCAGTCGCAAGAAATTTCCTCAGCTCCAGTGGCTAGCGCAAGCGATGCTTTAACACTTCCAAAAGAAGCACTAAATCACCCAGCAAATATTCACAATGTACTCATCTATGTCGCATCTTTGCTAGAGATGACCACAGAAGAACTTGCTGAAATAAGTTACCATAATGCAGTGCGCTTATTCTCCTACGATGTAGGAACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TatD_DNase

Protein Analysis

300

Amino Acids

32.92

Weight (kDa)

5.76

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TatD_DNase PF01026 5 - 294 4.7e-63 TatD related DNase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 589
AccB1I GGYRCC 2 cut(s) 58, 589
AccI GTMKAC 1 cut(s) 257
AccII CGCG 1 cut(s) 20
AciI CCGC 1 cut(s) 74
AclWI GGATC 1 cut(s) 336
AcsI RAATTY 2 cut(s) 503, 711
AcuI CTGAAG 2 cut(s) 407, 602
AcyI GRCGYC 2 cut(s) 59, 93
AfaI GTAC 2 cut(s) 591, 798
AfiI CCNNNNNNNGG 2 cut(s) 185, 225
AgsI TTSAA 2 cut(s) 359, 671
AluBI AGCT 3 cut(s) 151, 697, 722
AluI AGCT 3 cut(s) 151, 697, 722
Alw21I GWGCWC 1 cut(s) 699
Alw26I GTCTC 3 cut(s) 124, 194, 608
AlwI GGATC 1 cut(s) 336
AoxI GGCC 2 cut(s) 380, 443
ApeKI GCWGC 2 cut(s) 148, 269
ApoI RAATTY 2 cut(s) 503, 711
Asp718I GGTACC 1 cut(s) 589
AspA2I CCTAGG 1 cut(s) 478
AspLEI GCGC 4 cut(s) 22, 61, 737, 873
AspS9I GGNCC 2 cut(s) 216, 443
AsuHPI GGTGA 3 cut(s) 20, 425, 767
AsuNHI GCTAGC 1 cut(s) 731
AvaII GGWCC 1 cut(s) 216
AvrII CCTAGG 1 cut(s) 478
BanI GGYRCC 2 cut(s) 58, 589
BanII GRGCYC 1 cut(s) 699
Bbv12I GWGCWC 1 cut(s) 699
BbvCI CCTCAGC 1 cut(s) 718
BbvI GCAGC 2 cut(s) 160, 281
BccI CCATC 4 cut(s) 201, 398, 487, 580
BceAI ACGGC 1 cut(s) 92
BcoDI GTCTC 3 cut(s) 124, 194, 608
BfaI CTAG 3 cut(s) 479, 732, 821
BfmI CTRYAG 1 cut(s) 267
BfoI RGCGCY 1 cut(s) 62
BisI GCNGC 3 cut(s) 75, 149, 270
BlnI CCTAGG 1 cut(s) 478
BlsI GCNGC 3 cut(s) 76, 150, 271
Bme18I GGWCC 1 cut(s) 216
BmgT120I GGNCC 2 cut(s) 216, 443
BmiI GGNNCC 5 cut(s) 60, 218, 498, 591, 895
BmsI GCATC 7 cut(s) 395, 567, 610, 619, 667, 733, 821
BmtI GCTAGC 1 cut(s) 735
BpmI CTGGAG 3 cut(s) 632, 683, 708
Bpu10I CCTNAGC 1 cut(s) 718
BsaHI GRCGYC 2 cut(s) 59, 93
BsaI GGTCTC 1 cut(s) 194
BsaJI CCNNGG 2 cut(s) 197, 478
Bsc4I CCNNNNNNNGG 2 cut(s) 185, 225
Bse1I ACTGG 4 cut(s) 510, 615, 700, 725
BseDI CCNNGG 2 cut(s) 197, 478
BseGI GGATG 4 cut(s) 193, 386, 390, 582
BseLI CCNNNNNNNGG 2 cut(s) 185, 225
BseMII CTCAG 4 cut(s) 114, 480, 636, 732
BseNI ACTGG 4 cut(s) 510, 615, 700, 725
BseXI GCAGC 2 cut(s) 160, 281
BseYI CCCAGC 1 cut(s) 778
Bsh1236I CGCG 1 cut(s) 20
BshFI GGCC 2 cut(s) 382, 445
BshNI GGYRCC 2 cut(s) 58, 589
BsiHKAI GWGCWC 1 cut(s) 699
BslI CCNNNNNNNGG 2 cut(s) 185, 225
BsmAI GTCTC 3 cut(s) 124, 194, 608
BsnI GGCC 2 cut(s) 382, 445
Bso31I GGTCTC 1 cut(s) 194
Bsp1286I GDGCHC 1 cut(s) 699
Bsp143I GATC 1 cut(s) 328
BspACI CCGC 1 cut(s) 74
BspANI GGCC 2 cut(s) 382, 445
BspCNI CTCAG 4 cut(s) 115, 481, 637, 731
BspFNI CGCG 1 cut(s) 20
BspLI GGNNCC 5 cut(s) 60, 218, 498, 591, 895
BspMAI CTGCAG 1 cut(s) 271
BspOI GCTAGC 1 cut(s) 735
BspPI GGATC 1 cut(s) 336
BspT107I GGYRCC 2 cut(s) 58, 589
BspTNI GGTCTC 1 cut(s) 194
BsrI ACTGG 4 cut(s) 510, 615, 700, 725
BssECI CCNNGG 2 cut(s) 197, 478
BssMI GATC 1 cut(s) 328
BssNI GRCGYC 2 cut(s) 59, 93
BssT1I CCWWGG 2 cut(s) 197, 478
Bst4CI ACNGT 1 cut(s) 26
BstACI GRCGYC 2 cut(s) 59, 93
BstAPI GCANNNNNTGC 2 cut(s) 629, 743
BstC8I GCNNGC 4 cut(s) 384, 457, 733, 739
BstDEI CTNAG 4 cut(s) 123, 489, 645, 718
BstEII GGTNACC 1 cut(s) 413
BstF5I GGATG 4 cut(s) 193, 386, 390, 582
BstFNI CGCG 1 cut(s) 20
BstH2I RGCGCY 1 cut(s) 62
BstHHI GCGC 4 cut(s) 22, 61, 737, 873
BstKTI GATC 1 cut(s) 331
BstMAI GTCTC 3 cut(s) 124, 194, 608
BstMBI GATC 1 cut(s) 328
BstMWI GCNNNNNNNGC 4 cut(s) 629, 703, 728, 743
BstPI GGTNACC 1 cut(s) 413
BstSFI CTRYAG 1 cut(s) 267
BstUI CGCG 1 cut(s) 20
BstV1I GCAGC 2 cut(s) 160, 281
BsuRI GGCC 2 cut(s) 382, 445
BtgZI GCGATG 1 cut(s) 756
BtsCI GGATG 4 cut(s) 193, 386, 390, 582
BtsI GCAGTG 1 cut(s) 873
BtsIMutI CAGTG 3 cut(s) 22, 732, 873
Cac8I GCNNGC 4 cut(s) 384, 457, 733, 739
CfoI GCGC 4 cut(s) 22, 61, 737, 873
Cfr13I GGNCC 2 cut(s) 216, 443
CseI GACGC 2 cut(s) 26, 82
Csp6I GTAC 2 cut(s) 590, 797
CviAII CATG 4 cut(s) 180, 305, 547, 553
CviJI RGCY 8 cut(s) 151, 229, 288, 382, 445, 697, 722, 731
CviKI_1 RGCY 8 cut(s) 151, 229, 288, 382, 445, 697, 722, 731
CviQI GTAC 2 cut(s) 590, 797
DdeI CTNAG 4 cut(s) 123, 489, 645, 718
DinI GGCGCC 1 cut(s) 60
DpnI GATC 1 cut(s) 330
DpnII GATC 1 cut(s) 328
DraI TTTAAA 1 cut(s) 240
Ecl136II GAGCTC 1 cut(s) 697
Eco130I CCWWGG 2 cut(s) 197, 478
Eco24I GRGCYC 1 cut(s) 699
Eco31I GGTCTC 1 cut(s) 194
Eco47I GGWCC 1 cut(s) 216
Eco53kI GAGCTC 1 cut(s) 697
Eco57I CTGAAG 2 cut(s) 407, 602
Eco91I GGTNACC 1 cut(s) 413
EcoICRI GAGCTC 1 cut(s) 697
EcoO109I RGGNCCY 2 cut(s) 216, 443
EcoO65I GGTNACC 1 cut(s) 413
EcoT14I CCWWGG 2 cut(s) 197, 478
EcoT22I ATGCAT 1 cut(s) 634
EcoT38I GRGCYC 1 cut(s) 699
EgeI GGCGCC 1 cut(s) 60
EheI GGCGCC 1 cut(s) 60
ErhI CCWWGG 2 cut(s) 197, 478
FaeI CATG 4 cut(s) 183, 308, 550, 556
FaiI YATR 6 cut(s) 181, 306, 548, 554, 807, 861
FalI AAGNNNNNCTT 2 cut(s) 730, 762
FatI CATG 4 cut(s) 179, 304, 546, 552
FblI GTMKAC 1 cut(s) 257
Fnu4HI GCNGC 3 cut(s) 75, 149, 270
FokI GGATG 4 cut(s) 180, 373, 377, 589
FriOI GRGCYC 1 cut(s) 699
Fsp4HI GCNGC 3 cut(s) 75, 149, 270
FspBI CTAG 3 cut(s) 479, 732, 821
GlaI GCGC 4 cut(s) 21, 60, 736, 872
GluI GCNGC 3 cut(s) 75, 149, 270
GsaI CCCAGC 1 cut(s) 782
GsuI CTGGAG 3 cut(s) 632, 683, 708
HaeII RGCGCY 1 cut(s) 62
HaeIII GGCC 2 cut(s) 382, 445
HgaI GACGC 2 cut(s) 26, 82
HhaI GCGC 4 cut(s) 22, 61, 737, 873
Hin1I GRCGYC 2 cut(s) 59, 93
Hin1II CATG 4 cut(s) 183, 308, 550, 556
Hin6I GCGC 4 cut(s) 20, 59, 735, 871
HinP1I GCGC 4 cut(s) 20, 59, 735, 871
HinfI GANTC 6 cut(s) 45, 86, 117, 158, 254, 463
HphI GGTGA 3 cut(s) 20, 425, 767
Hpy166II GTNNAC 2 cut(s) 258, 562
Hpy188I TCNGA 4 cut(s) 124, 426, 598, 646
Hpy188III TCNNGA 2 cut(s) 500, 537
Hpy8I GTNNAC 2 cut(s) 258, 562
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 5 cut(s) 307, 428, 603, 665, 694
HpyCH4III ACNGT 1 cut(s) 26
HpyCH4V TGCA 6 cut(s) 209, 269, 365, 623, 632, 866
HpyF10VI GCNNNNNNNGC 4 cut(s) 629, 703, 728, 743
HpyF3I CTNAG 4 cut(s) 123, 489, 645, 718
Hsp92I GRCGYC 2 cut(s) 59, 93
Hsp92II CATG 4 cut(s) 183, 308, 550, 556
HspAI GCGC 4 cut(s) 20, 59, 735, 871
KasI GGCGCC 1 cut(s) 58
KpnI GGTACC 1 cut(s) 593
Kzo9I GATC 1 cut(s) 328
LmnI GCTCC 2 cut(s) 702, 727
Lsp1109I GCAGC 2 cut(s) 160, 281
LweI GCATC 7 cut(s) 395, 567, 610, 619, 667, 733, 821
MaeI CTAG 3 cut(s) 479, 732, 821
MaeIII GTNAC 3 cut(s) 26, 413, 854
MalI GATC 1 cut(s) 330
MboI GATC 1 cut(s) 328
MboII GAAGA 7 cut(s) 40, 241, 322, 413, 458, 595, 848
MfeI CAATTG 1 cut(s) 65
MhlI GDGCHC 1 cut(s) 699
MluCI AATT 7 cut(s) 8, 65, 174, 223, 245, 503, 711
Mly113I GGCGCC 1 cut(s) 59
MlyI GAGTC 3 cut(s) 126, 167, 263
MmeI TCCRAC 1 cut(s) 256
MnlI CCTC 5 cut(s) 92, 207, 274, 567, 727
Mph1103I ATGCAT 1 cut(s) 634
MseI TTAA 5 cut(s) 231, 239, 336, 374, 749
MslI CAYNNNNRTG 1 cut(s) 551
MunI CAATTG 1 cut(s) 65
MvnI CGCG 1 cut(s) 20
MwoI GCNNNNNNNGC 4 cut(s) 629, 703, 728, 743
NarI GGCGCC 1 cut(s) 59
NdeII GATC 1 cut(s) 328
NheI GCTAGC 1 cut(s) 731
NlaIII CATG 4 cut(s) 183, 308, 550, 556
NlaIV GGNNCC 5 cut(s) 60, 218, 498, 591, 895
NmuCI GTSAC 2 cut(s) 26, 413
NsiI ATGCAT 1 cut(s) 634
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PfeI GAWTC 3 cut(s) 45, 86, 463
PkrI GCNGC 3 cut(s) 76, 150, 271
PleI GAGTC 3 cut(s) 125, 166, 262
PluTI GGCGCC 1 cut(s) 62
PpsI GAGTC 3 cut(s) 125, 166, 262
PpuMI RGGWCCY 1 cut(s) 216
Psp124BI GAGCTC 1 cut(s) 699
Psp5II RGGWCCY 1 cut(s) 216
PspEI GGTNACC 1 cut(s) 413
PspFI CCCAGC 1 cut(s) 778
PspN4I GGNNCC 5 cut(s) 60, 218, 498, 591, 895
PspPI GGNCC 2 cut(s) 216, 443
PspPPI RGGWCCY 1 cut(s) 216
PstI CTGCAG 1 cut(s) 271
RsaI GTAC 2 cut(s) 591, 798
RsaNI GTAC 2 cut(s) 590, 797
RseI CAYNNNNRTG 1 cut(s) 551
SacI GAGCTC 1 cut(s) 699
SaqAI TTAA 5 cut(s) 231, 239, 336, 374, 749
SatI GCNGC 3 cut(s) 75, 149, 270
Sau3AI GATC 1 cut(s) 328
Sau96I GGNCC 2 cut(s) 216, 443
SchI GAGTC 3 cut(s) 126, 167, 263
SduI GDGCHC 1 cut(s) 699
SfaNI GCATC 7 cut(s) 395, 567, 610, 619, 667, 733, 821
SfcI CTRYAG 1 cut(s) 267
SfoI GGCGCC 1 cut(s) 60
SinI GGWCC 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 551
Sse9I AATT 7 cut(s) 8, 65, 174, 223, 245, 503, 711
SsiI CCGC 1 cut(s) 74
SspDI GGCGCC 1 cut(s) 58
SspI AATATT 2 cut(s) 606, 787
SspMI CTAG 3 cut(s) 479, 732, 821
SstI GAGCTC 1 cut(s) 699
StyI CCWWGG 2 cut(s) 197, 478
TaaI ACNGT 1 cut(s) 26
TaqI TCGA 4 cut(s) 51, 84, 252, 318
TaqII GACCGA 1 cut(s) 176
TasI AATT 7 cut(s) 8, 65, 174, 223, 245, 503, 711
TatI WGTACW 1 cut(s) 796
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 3 cut(s) 45, 86, 463
Tru1I TTAA 5 cut(s) 231, 239, 336, 374, 749
Tru9I TTAA 5 cut(s) 231, 239, 336, 374, 749
TscAI CASTG 3 cut(s) 29, 732, 873
TseFI GTSAC 2 cut(s) 26, 413
TseI GCWGC 2 cut(s) 148, 269
Tsp45I GTSAC 2 cut(s) 26, 413
TspDTI ATGAA 4 cut(s) 20, 446, 458, 569
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 3 cut(s) 29, 732, 873
VpaK11BI GGWCC 1 cut(s) 216
XapI RAATTY 2 cut(s) 503, 711
XmaJI CCTAGG 1 cut(s) 478
XmiI GTMKAC 1 cut(s) 257
XspI CTAG 3 cut(s) 479, 732, 821
Zsp2I ATGCAT 1 cut(s) 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.