AT3G03700

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004978
Physical Location & Seq
Forward (+)
916435 .. 918490
2056 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G03700.1

Sequence Viewer

Length: 1449 bp
ATGCCTACACCAACAAGCAACGGTCATGCCTCTCGCGTGCAGATTCAAGAACCAAGAATAATGTCTCCTCTTCCTCCTTCTTCTTCTCCAATCGCCTTCAAGGAACAACAAGGTAGACCACCTCCAACAACACAACAAACCATAGCAGGAAAACTCTTTAGAACTCTTTTCAAGGGTCTTCTCTTCTCACAACTAACCTTAATCTCACTTTTGGTGATCGTTCTCACCATTCGCGGTCTCATCTCAGCAAGTACACACCATTTCCACCTCAAGAAATGGTACCCTCCTTTACTAGCATCTGTTGCTGTCTCAGGAATTGCATCTTTAGCATGGCAATGCATCTTTATCTACAATCCATCAAGAGCAGTCAAAGCAACGTTCTGGCTTAGTCCAATACTCACCTGCTCGGTAGGAATCTTGCTTGTTTTGATTGGCTCAGCGGTAGATGCAGGTATAGGTGCAGTGTTTGTCCTTTTCGCCATTACTCAGTCCCTCTATGGTTGCTGGATTACTCCGAGGCTTGAGTACACCGATAAAATATTATCACTTGCCACAGCATTTCCACCTGCAAGAACCAGAGAAGTAGTCTGCTTATCAATCATAGTCAGTGTCGTTTACTCTGGTTTCTTGGTGACTGGAATTGGAGGAGCAACTTCCACTAGAACAAATCTTGATATCTTGTTCATATCCGTAATCATAATAAGCTTAGCATGGACGATGCAAGTTATCAAGAATGTTCAACAAGTTGCGATTTCACGGGCGAGATATGTAAACTTTGCACATGGAGAAGATATGGACGCTTGGAATGCTTTCCGCATCACTATGAAACACTTGACTGGGAGCATCTGCGTTGGCTCGACGCTTGTTCCAATCATCGTATTCATAAGAGGATCAATAAGAAGTGTTAATCTAATGTCAGGGAGCAGCGATGAGGTCATGTATTCAGGTGCTGATTGCTTCTCTACCATTGCTAACAAACTCATTACACTTGGAAACAGATGGGGCTTTGTACATGTTGGAACCTATGATAAAGGATTCATGGAGGCTTCGAGTGATACCTGGAAGAAGTTCAGAAGCACAACAGGGTTGGAGAAACTGATTGACTCGGATCTCACTAGCTCCTTCTGTTTTCTCAGTGCTGTTTCTGTTGGAGCTGTCTCTTCATTAACTGCTGGGATATGGATGTTGTTAATACACAAGGACTATGCCCTGGAAGTGACTCTCTATGCTTTCATAATTGGATATTTTGTGGGAAGGGTAGGTTTGGCGTGGCTACAGGCATGTGTATTGGCTTACTATGTAGCTTATTCTGAAGATCCTCAGAGCATGAGATTTGATGGAACAATTCCACAACGTATTCAGCGTCTTCAAATGTTAAGTGCTCACCGAGATAACAGGGAGCTTGAGGTGGGTAGAGACAGAGAAGAAGATAGTTATAACAATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

482

Amino Acids

53.05

Weight (kDa)

9.07

Isoelectric Point (pI)

31.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 224 - 441 1.1e-24 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1437
AarI CACCTGC 2 cut(s) 410, 574
Acc36I ACCTGC 3 cut(s) 410, 440, 574
Acc65I GGTACC 1 cut(s) 279
AccB1I GGYRCC 1 cut(s) 279
AccI GTMKAC 1 cut(s) 115
AccII CGCG 2 cut(s) 36, 234
AciI CCGC 3 cut(s) 234, 440, 814
AclI AACGTT 1 cut(s) 377
AclWI GGATC 3 cut(s) 898, 1116, 1310
AcuI CTGAAG 1 cut(s) 1332
AfaI GTAC 4 cut(s) 253, 281, 527, 1011
AflIII ACRYGT 1 cut(s) 1012
AgsI TTSAA 5 cut(s) 47, 100, 172, 740, 1370
AjnI CCWGG 2 cut(s) 1058, 1209
AluBI AGCT 5 cut(s) 705, 1119, 1154, 1304, 1402
AluI AGCT 5 cut(s) 705, 1119, 1154, 1304, 1402
Alw21I GWGCWC 1 cut(s) 1384
Alw26I GTCTC 5 cut(s) 69, 242, 313, 1162, 1410
AlwI GGATC 3 cut(s) 898, 1116, 1310
AlwNI CAGNNNCTG 1 cut(s) 950
ApeKI GCWGC 1 cut(s) 924
Asp700I GAANNNNTTC 2 cut(s) 809, 1067
Asp718I GGTACC 1 cut(s) 279
AsuHPI GGTGA 5 cut(s) 217, 226, 391, 643, 1376
BanI GGYRCC 1 cut(s) 279
BbsI GAAGAC 2 cut(s) 170, 1358
Bbv12I GWGCWC 1 cut(s) 1384
BbvI GCAGC 1 cut(s) 936
BccI CCATC 3 cut(s) 364, 993, 1331
BciT130I CCWGG 2 cut(s) 1060, 1211
BcoDI GTCTC 5 cut(s) 69, 242, 313, 1162, 1410
BfaI CTAG 3 cut(s) 293, 660, 1116
BfmI CTRYAG 1 cut(s) 1274
BfuAI ACCTGC 3 cut(s) 410, 440, 574
BisI GCNGC 1 cut(s) 925
BlpI GCTNAGC 2 cut(s) 436, 706
BlsI GCNGC 1 cut(s) 926
Bme1390I CCNGG 2 cut(s) 1060, 1211
BmiI GGNNCC 2 cut(s) 281, 1021
BmrFI CCNGG 2 cut(s) 1060, 1211
BmrI ACTGGG 1 cut(s) 846
BmsI GCATC 7 cut(s) 305, 329, 348, 436, 708, 825, 852
BmuI ACTGGG 1 cut(s) 846
BpiI GAAGAC 2 cut(s) 170, 1358
Bpu1102I GCTNAGC 2 cut(s) 436, 706
BpuEI CTTGAG 3 cut(s) 254, 542, 1424
BsaI GGTCTC 1 cut(s) 242
BsaJI CCNNGG 2 cut(s) 515, 1209
Bse1I ACTGG 2 cut(s) 640, 841
Bse3DI GCAATG 2 cut(s) 341, 966
BseBI CCWGG 2 cut(s) 1060, 1211
BseDI CCNNGG 2 cut(s) 515, 1209
BseGI GGATG 1 cut(s) 1188
BseMI GCAATG 2 cut(s) 341, 966
BseMII CTCAG 6 cut(s) 258, 324, 450, 500, 1147, 1334
BseNI ACTGG 2 cut(s) 640, 841
BseRI GAGGAG 2 cut(s) 57, 660
BseXI GCAGC 1 cut(s) 936
BseYI CCCAGC 1 cut(s) 1172
BsgI GTGCAG 2 cut(s) 59, 480
Bsh1236I CGCG 2 cut(s) 36, 234
BshNI GGYRCC 1 cut(s) 279
BsiHKAI GWGCWC 1 cut(s) 1384
BslFI GGGAC 1 cut(s) 475
BsmAI GTCTC 5 cut(s) 69, 242, 313, 1162, 1410
BsmFI GGGAC 1 cut(s) 475
BsmI GAATGC 1 cut(s) 811
Bso31I GGTCTC 1 cut(s) 242
Bsp1286I GDGCHC 1 cut(s) 1384
Bsp1407I TGTACA 1 cut(s) 1009
Bsp143I GATC 4 cut(s) 216, 890, 1108, 1315
Bsp1720I GCTNAGC 2 cut(s) 436, 706
BspACI CCGC 3 cut(s) 234, 440, 814
BspCNI CTCAG 6 cut(s) 257, 323, 449, 499, 1146, 1333
BspFNI CGCG 2 cut(s) 36, 234
BspLI GGNNCC 2 cut(s) 281, 1021
BspMI ACCTGC 3 cut(s) 410, 440, 574
BspPI GGATC 3 cut(s) 898, 1116, 1310
BspT107I GGYRCC 1 cut(s) 279
BspTNI GGTCTC 1 cut(s) 242
BsrDI GCAATG 2 cut(s) 341, 966
BsrGI TGTACA 1 cut(s) 1009
BsrI ACTGG 2 cut(s) 640, 841
BssECI CCNNGG 2 cut(s) 515, 1209
BssMI GATC 4 cut(s) 216, 890, 1108, 1315
Bst2UI CCWGG 2 cut(s) 1060, 1211
Bst4CI ACNGT 1 cut(s) 23
Bst6I CTCTTC 3 cut(s) 75, 188, 1165
BstAPI GCANNNNNTGC 1 cut(s) 302
BstAUI TGTACA 1 cut(s) 1009
BstC8I GCNNGC 1 cut(s) 38
BstDEI CTNAG 8 cut(s) 244, 310, 386, 436, 486, 706, 1133, 1320
BstF5I GGATG 1 cut(s) 1188
BstFNI CGCG 2 cut(s) 36, 234
BstKTI GATC 4 cut(s) 219, 893, 1111, 1318
BstMAI GTCTC 5 cut(s) 69, 242, 313, 1162, 1410
BstMBI GATC 4 cut(s) 216, 890, 1108, 1315
BstMWI GCNNNNNNNGC 5 cut(s) 302, 326, 371, 446, 806
BstNI CCWGG 2 cut(s) 1060, 1211
BstNSI RCATGY 2 cut(s) 1016, 1284
BstSCI CCNGG 2 cut(s) 1058, 1209
BstSFI CTRYAG 1 cut(s) 1274
BstUI CGCG 2 cut(s) 36, 234
BstV1I GCAGC 1 cut(s) 936
BstV2I GAAGAC 2 cut(s) 170, 1358
BstX2I RGATCY 2 cut(s) 1108, 1315
BstYI RGATCY 2 cut(s) 1108, 1315
BtgZI GCGATG 1 cut(s) 942
BtsCI GGATG 1 cut(s) 1188
BtsI GCAGTG 1 cut(s) 468
BtsIMutI CAGTG 3 cut(s) 468, 613, 1141
BveI ACCTGC 3 cut(s) 410, 440, 574
Cac8I GCNNGC 1 cut(s) 38
CaiI CAGNNNCTG 1 cut(s) 950
CseI GACGC 3 cut(s) 806, 868, 1352
Csp6I GTAC 4 cut(s) 252, 280, 526, 1010
CviAII CATG 9 cut(s) 26, 330, 711, 782, 937, 1013, 1039, 1281, 1327
CviQI GTAC 4 cut(s) 252, 280, 526, 1010
DdeI CTNAG 8 cut(s) 244, 310, 386, 436, 486, 706, 1133, 1320
DpnI GATC 4 cut(s) 218, 892, 1110, 1317
DpnII GATC 4 cut(s) 216, 890, 1108, 1315
Eam1104I CTCTTC 3 cut(s) 75, 188, 1165
EarI CTCTTC 3 cut(s) 75, 188, 1165
Eco31I GGTCTC 1 cut(s) 242
Eco32I GATATC 1 cut(s) 676
Eco57I CTGAAG 1 cut(s) 1332
EcoRII CCWGG 2 cut(s) 1058, 1209
EcoRV GATATC 1 cut(s) 676
EcoT22I ATGCAT 1 cut(s) 341
FaeI CATG 9 cut(s) 29, 333, 714, 785, 940, 1016, 1042, 1284, 1330
FaqI GGGAC 1 cut(s) 475
FatI CATG 9 cut(s) 25, 329, 710, 781, 936, 1012, 1038, 1280, 1326
FblI GTMKAC 1 cut(s) 115
Fnu4HI GCNGC 1 cut(s) 925
FokI GGATG 1 cut(s) 1195
Fsp4HI GCNGC 1 cut(s) 925
FspBI CTAG 3 cut(s) 293, 660, 1116
GluI GCNGC 1 cut(s) 925
GsaI CCCAGC 1 cut(s) 1176
HgaI GACGC 3 cut(s) 806, 868, 1352
Hin1II CATG 9 cut(s) 29, 333, 714, 785, 940, 1016, 1042, 1284, 1330
HindIII AAGCTT 1 cut(s) 703
HinfI GANTC 5 cut(s) 43, 414, 1035, 1103, 1219
HphI GGTGA 5 cut(s) 217, 226, 391, 643, 1376
Hpy166II GTNNAC 5 cut(s) 116, 254, 528, 616, 772
Hpy188I TCNGA 5 cut(s) 516, 1073, 1108, 1312, 1323
Hpy188III TCNNGA 6 cut(s) 47, 271, 312, 360, 671, 730
Hpy8I GTNNAC 5 cut(s) 116, 254, 528, 616, 772
Hpy99I CGWCG 1 cut(s) 862
HpyAV CCTTC 4 cut(s) 87, 106, 1132, 1248
HpyCH4III ACNGT 1 cut(s) 23
HpyCH4IV ACGT 2 cut(s) 377, 1354
HpyCH4V TGCA 8 cut(s) 40, 320, 339, 449, 461, 569, 721, 779
HpyF10VI GCNNNNNNNGC 5 cut(s) 302, 326, 371, 446, 806
HpyF3I CTNAG 8 cut(s) 244, 310, 386, 436, 486, 706, 1133, 1320
HpySE526I ACGT 2 cut(s) 377, 1354
Hsp92II CATG 9 cut(s) 29, 333, 714, 785, 940, 1016, 1042, 1284, 1330
KpnI GGTACC 1 cut(s) 283
Kzo9I GATC 4 cut(s) 216, 890, 1108, 1315
LmnI GCTCC 6 cut(s) 647, 840, 921, 1124, 1151, 1399
Lsp1109I GCAGC 1 cut(s) 936
LweI GCATC 7 cut(s) 305, 329, 348, 436, 708, 825, 852
MaeI CTAG 3 cut(s) 293, 660, 1116
MaeII ACGT 2 cut(s) 377, 1354
MaeIII GTNAC 2 cut(s) 631, 1216
MalI GATC 4 cut(s) 218, 892, 1110, 1317
MboI GATC 4 cut(s) 216, 890, 1108, 1315
MfeI CAATTG 1 cut(s) 1441
MflI RGATCY 2 cut(s) 1108, 1315
MhlI GDGCHC 1 cut(s) 1384
MluCI AATT 5 cut(s) 315, 639, 1236, 1344, 1441
MlyI GAGTC 2 cut(s) 1097, 1213
MmeI TCCRAC 4 cut(s) 149, 997, 1068, 1129
Mph1103I ATGCAT 1 cut(s) 341
MroXI GAANNNNTTC 2 cut(s) 809, 1067
MseI TTAA 5 cut(s) 200, 906, 1166, 1190, 1376
MslI CAYNNNNRTG 2 cut(s) 334, 821
MspA1I CMGCKG 1 cut(s) 440
MspR9I CCNGG 2 cut(s) 1060, 1211
MunI CAATTG 1 cut(s) 1441
Mva1269I GAATGC 1 cut(s) 811
MvaI CCWGG 2 cut(s) 1060, 1211
MvnI CGCG 2 cut(s) 36, 234
MwoI GCNNNNNNNGC 5 cut(s) 302, 326, 371, 446, 806
NdeII GATC 4 cut(s) 216, 890, 1108, 1315
NlaIII CATG 9 cut(s) 29, 333, 714, 785, 940, 1016, 1042, 1284, 1330
NlaIV GGNNCC 2 cut(s) 281, 1021
NmuCI GTSAC 2 cut(s) 631, 1216
NsiI ATGCAT 1 cut(s) 341
NspI RCATGY 2 cut(s) 1016, 1284
PaqCI CACCTGC 2 cut(s) 410, 574
PciI ACATGT 1 cut(s) 1012
PcsI WCGNNNNNNNCGW 1 cut(s) 1360
PctI GAATGC 1 cut(s) 811
PdmI GAANNNNTTC 2 cut(s) 809, 1067
PfeI GAWTC 3 cut(s) 43, 414, 1035
PkrI GCNGC 1 cut(s) 926
PleI GAGTC 2 cut(s) 1097, 1213
PpsI GAGTC 2 cut(s) 1097, 1213
PscI ACATGT 1 cut(s) 1012
PsiI TTATAA 1 cut(s) 1437
Psp1406I AACGTT 1 cut(s) 377
Psp6I CCWGG 2 cut(s) 1058, 1209
PspFI CCCAGC 1 cut(s) 1172
PspGI CCWGG 2 cut(s) 1058, 1209
PspN4I GGNNCC 2 cut(s) 281, 1021
PstNI CAGNNNCTG 1 cut(s) 950
PsuI RGATCY 2 cut(s) 1108, 1315
RsaI GTAC 4 cut(s) 253, 281, 527, 1011
RsaNI GTAC 4 cut(s) 252, 280, 526, 1010
RseI CAYNNNNRTG 2 cut(s) 334, 821
SaqAI TTAA 5 cut(s) 200, 906, 1166, 1190, 1376
SatI GCNGC 1 cut(s) 925
Sau3AI GATC 4 cut(s) 216, 890, 1108, 1315
SchI GAGTC 2 cut(s) 1097, 1213
ScrFI CCNGG 2 cut(s) 1060, 1211
SduI GDGCHC 1 cut(s) 1384
SfaNI GCATC 7 cut(s) 305, 329, 348, 436, 708, 825, 852
SfcI CTRYAG 1 cut(s) 1274
SmiMI CAYNNNNRTG 2 cut(s) 334, 821
SmlI CTYRAG 3 cut(s) 269, 521, 1403
SmoI CTYRAG 3 cut(s) 269, 521, 1403
Sse9I AATT 5 cut(s) 315, 639, 1236, 1344, 1441
SsiI CCGC 3 cut(s) 234, 440, 814
SspI AATATT 1 cut(s) 540
SspMI CTAG 3 cut(s) 293, 660, 1116
StyD4I CCNGG 2 cut(s) 1058, 1209
TaaI ACNGT 1 cut(s) 23
TaiI ACGT 2 cut(s) 380, 1357
TaqI TCGA 2 cut(s) 857, 1049
TasI AATT 5 cut(s) 315, 639, 1236, 1344, 1441
TatI WGTACW 3 cut(s) 251, 525, 1009
TfiI GAWTC 3 cut(s) 43, 414, 1035
Tru1I TTAA 5 cut(s) 200, 906, 1166, 1190, 1376
Tru9I TTAA 5 cut(s) 200, 906, 1166, 1190, 1376
TscAI CASTG 3 cut(s) 468, 613, 1141
TseFI GTSAC 2 cut(s) 631, 1216
TseI GCWGC 1 cut(s) 924
Tsp45I GTSAC 2 cut(s) 631, 1216
TspDTI ATGAA 6 cut(s) 673, 839, 871, 1027, 1152, 1222
TspGWI ACGGA 1 cut(s) 679
TspRI CASTG 3 cut(s) 468, 613, 1141
XceI RCATGY 2 cut(s) 1016, 1284
XmiI GTMKAC 1 cut(s) 115
XmnI GAANNNNTTC 2 cut(s) 809, 1067
XspI CTAG 3 cut(s) 293, 660, 1116
Zsp2I ATGCAT 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.