Rh3CG358200

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0004978
Physical Location & Seq
Forward (+)
44235595 .. 44237175
1581 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG358200.1

Sequence Viewer

Length: 1221 bp
ATGGCAACTACAGAGAACATCCTCAAACAAGTTCAAGAAGAAGTAGGCCACGAACCGCATCAAGTTTACGGTCTCATTTCCACCAATCACAAGCACCATTTCCACCCACTACAGTTCTATGCTCCACTGCTCACTTCAACACTCTGCTCTGCAATTGTCGCTTTAATATGGCAATGGGTCACCAGGATAAGCCCTTCGAAAGCCATCAAAACAGCCTTTTGGCTCAGCCCCTTGCTAACCTGCGCAGTTGGTGTACTTCTCATATCTATAAGTTCTGCTGGGAGTTTGGTTGCAGGTGGGGTTGCAATCATCTGTGCGCTCATTCAATCGCTGTATGCCTGCTGGGTCAGTCCCCGTATCGAATTTGCCATCAGGATATTATCTGTTTCTACAGCTGTCCCTCCTCCAAAAATCACAACATTACTCATTCTATCAATTTTTACCTGTATCCTCTACCTCTCTTTCTTGGTATCCGGCATCGGAGGAGCCACTGCCATAAGAAGCAGCTTTAGTGTCCTGTTTATCTCGGTTATCTTGCTGAGCATGGCATGGACTATGCATCTCATCAAGAACCTGCTGCAAGTCACAATTTCACGCATCAAGTACATGCACTTTGCATGCGGAGAAGATGTTGACACAAGTGAGGCTTTCTGCAACACAGTTAAGCACTCAATGGGGAGTATCTGCATTGGCTCCATCTTGGTTCCAGTTCTCGGAGTTATTCGGGGATCAGCCAGAGGCATGAAATTGGTTGCAGGGGATACAGACGAGATTCTATTTTCATGCGCCAACTGCTATTCGGGACTGGCTTCGACTCTGGTGATGTATGGGAATCGCTGGGGTTTTGTGCATGTTGGAGTCTATAACAAGGGCTTTGTGCAGGCATCAGCTGATACATGGGAGATGTTCAGGAATATAGAACTAAGAGAAATAATCGACTTAGATCTCACTACATCGTTCTGTTTTCTTTCTGGGGTGGTCGGAGGTGCAATATGTAGCCTAGTCAGTGGAACTTGGACTCTTGCAATTCATAAGAGTTATGCTACTGAAGTCTCCTTATATGCATTCTTGATTGGCTATTTCATGTGCAGGATTGCTGTGGCATGGCCACAAGCATGTGTTTTGTCTTACTACGTAGCTTATGCAGAGAACCCTAATAGCACGCGATTTGACTCAACACTTCCCGTTCGCATTGAAGAGCTTCGGAGATTTCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

406

Amino Acids

44.48

Weight (kDa)

8.16

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 101 - 385 4.9e-27 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 284
Acc16I TGCGCA 1 cut(s) 244
Acc36I ACCTGC 3 cut(s) 248, 284, 582
AccII CGCG 1 cut(s) 1165
AciI CCGC 2 cut(s) 56, 621
AclWI GGATC 1 cut(s) 736
AcoI YGGCCR 1 cut(s) 1106
AcsI RAATTY 1 cut(s) 362
AcuI CTGAAG 1 cut(s) 1068
AfaI GTAC 2 cut(s) 255, 605
AfiI CCNNNNNNNGG 1 cut(s) 713
AgsI TTSAA 5 cut(s) 35, 138, 326, 1196, 1214
AjnI CCWGG 1 cut(s) 182
AluBI AGCT 6 cut(s) 395, 507, 890, 1139, 1201, 1217
AluI AGCT 6 cut(s) 395, 507, 890, 1139, 1201, 1217
Alw26I GTCTC 2 cut(s) 77, 1057
AlwI GGATC 1 cut(s) 736
AoxI GGCC 2 cut(s) 46, 1106
ApeKI GCWGC 2 cut(s) 504, 577
ApoI RAATTY 1 cut(s) 362
Asp700I GAANNNNTTC 1 cut(s) 1200
AspLEI GCGC 3 cut(s) 245, 319, 788
AsuHPI GGTGA 2 cut(s) 172, 832
AsuII TTCGAA 1 cut(s) 197
BalI TGGCCA 1 cut(s) 1108
BbvI GCAGC 2 cut(s) 516, 564
BccI CCATC 3 cut(s) 212, 377, 704
BciT130I CCWGG 1 cut(s) 184
BciVI GTATCC 3 cut(s) 458, 481, 754
BcoDI GTCTC 2 cut(s) 77, 1057
BfaI CTAG 1 cut(s) 1001
BfmI CTRYAG 3 cut(s) 9, 110, 390
BfuAI ACCTGC 3 cut(s) 248, 284, 582
BfuI GTATCC 3 cut(s) 458, 481, 754
BglII AGATCT 1 cut(s) 943
BisI GCNGC 2 cut(s) 505, 578
BlpI GCTNAGC 2 cut(s) 224, 539
BlsI GCNGC 2 cut(s) 506, 579
Bme1390I CCNGG 1 cut(s) 184
BmiI GGNNCC 3 cut(s) 487, 694, 705
BmrFI CCNGG 1 cut(s) 184
BmsI GCATC 5 cut(s) 67, 486, 568, 606, 893
Bpu1102I GCTNAGC 2 cut(s) 224, 539
Bpu14I TTCGAA 1 cut(s) 197
BsaAI YACGTR 1 cut(s) 1135
BsaI GGTCTC 1 cut(s) 77
BsaXI ACNNNNNCTCC 2 cut(s) 615, 645
Bsc4I CCNNNNNNNGG 1 cut(s) 713
Bse1I ACTGG 2 cut(s) 707, 810
Bse3DI GCAATG 1 cut(s) 179
BseBI CCWGG 1 cut(s) 184
BseGI GGATG 1 cut(s) 18
BseLI CCNNNNNNNGG 1 cut(s) 713
BseMI GCAATG 1 cut(s) 179
BseMII CTCAG 2 cut(s) 238, 530
BseNI ACTGG 2 cut(s) 707, 810
BseRI GAGGAG 2 cut(s) 393, 498
BseXI GCAGC 2 cut(s) 516, 564
BseYI CCCAGC 3 cut(s) 278, 342, 837
BsgI GTGCAG 2 cut(s) 899, 1108
Bsh1236I CGCG 1 cut(s) 1165
BshFI GGCC 2 cut(s) 48, 1108
BsiSI CCGG 1 cut(s) 474
BslFI GGGAC 3 cut(s) 336, 383, 816
BslI CCNNNNNNNGG 1 cut(s) 713
BsmAI GTCTC 2 cut(s) 77, 1057
BsmFI GGGAC 3 cut(s) 336, 383, 816
BsmI GAATGC 1 cut(s) 1064
BsnI GGCC 2 cut(s) 48, 1108
Bso31I GGTCTC 1 cut(s) 77
Bsp119I TTCGAA 1 cut(s) 197
Bsp143I GATC 2 cut(s) 728, 943
Bsp1720I GCTNAGC 2 cut(s) 224, 539
BspACI CCGC 2 cut(s) 56, 621
BspANI GGCC 2 cut(s) 48, 1108
BspCNI CTCAG 2 cut(s) 237, 531
BspFNI CGCG 1 cut(s) 1165
BspLI GGNNCC 3 cut(s) 487, 694, 705
BspMI ACCTGC 3 cut(s) 248, 284, 582
BspPI GGATC 1 cut(s) 736
BspQI GCTCTTC 1 cut(s) 1191
BspT104I TTCGAA 1 cut(s) 197
BspTNI GGTCTC 1 cut(s) 77
BsrDI GCAATG 1 cut(s) 179
BsrI ACTGG 2 cut(s) 707, 810
BssMI GATC 2 cut(s) 728, 943
Bst2UI CCWGG 1 cut(s) 184
Bst4CI ACNGT 3 cut(s) 71, 114, 661
Bst6I CTCTTC 1 cut(s) 1191
BstBAI YACGTR 1 cut(s) 1135
BstBI TTCGAA 1 cut(s) 197
BstC8I GCNNGC 4 cut(s) 340, 619, 882, 1163
BstDEI CTNAG 4 cut(s) 224, 539, 923, 940
BstEII GGTNACC 1 cut(s) 178
BstF5I GGATG 1 cut(s) 18
BstFNI CGCG 1 cut(s) 1165
BstHHI GCGC 3 cut(s) 245, 319, 788
BstKTI GATC 2 cut(s) 731, 946
BstMAI GTCTC 2 cut(s) 77, 1057
BstMBI GATC 2 cut(s) 728, 943
BstMWI GCNNNNNNNGC 2 cut(s) 158, 792
BstNI CCWGG 1 cut(s) 184
BstNSI RCATGY 4 cut(s) 610, 621, 854, 1119
BstPI GGTNACC 1 cut(s) 178
BstSCI CCNGG 1 cut(s) 182
BstSFI CTRYAG 3 cut(s) 9, 110, 390
BstSNI TACGTA 1 cut(s) 1135
BstUI CGCG 1 cut(s) 1165
BstV1I GCAGC 2 cut(s) 516, 564
BstX2I RGATCY 1 cut(s) 943
BstYI RGATCY 1 cut(s) 943
BsuI GTATCC 3 cut(s) 458, 481, 754
BsuRI GGCC 2 cut(s) 48, 1108
BtsCI GGATG 1 cut(s) 18
BtsI GCAGTG 2 cut(s) 125, 489
BtsIMutI CAGTG 3 cut(s) 125, 489, 1012
BveI ACCTGC 3 cut(s) 248, 284, 582
Cac8I GCNNGC 4 cut(s) 340, 619, 882, 1163
CfoI GCGC 3 cut(s) 245, 319, 788
Csp6I GTAC 2 cut(s) 254, 604
CviQI GTAC 2 cut(s) 254, 604
DdeI CTNAG 4 cut(s) 224, 539, 923, 940
DpnI GATC 2 cut(s) 730, 945
DpnII GATC 2 cut(s) 728, 943
EaeI YGGCCR 1 cut(s) 1106
Eam1104I CTCTTC 1 cut(s) 1191
EarI CTCTTC 1 cut(s) 1191
Eco105I TACGTA 1 cut(s) 1135
Eco31I GGTCTC 1 cut(s) 77
Eco57I CTGAAG 1 cut(s) 1068
Eco91I GGTNACC 1 cut(s) 178
EcoO65I GGTNACC 1 cut(s) 178
EcoRII CCWGG 1 cut(s) 182
EcoT22I ATGCAT 2 cut(s) 561, 1066
FalI AAGNNNNNCTT 2 cut(s) 631, 663
FaqI GGGAC 3 cut(s) 336, 383, 816
Fnu4HI GCNGC 2 cut(s) 505, 578
FokI GGATG 1 cut(s) 5
Fsp4HI GCNGC 2 cut(s) 505, 578
FspBI CTAG 1 cut(s) 1001
FspI TGCGCA 1 cut(s) 244
GlaI GCGC 3 cut(s) 244, 318, 787
GluI GCNGC 2 cut(s) 505, 578
GsaI CCCAGC 3 cut(s) 282, 346, 841
HaeIII GGCC 2 cut(s) 48, 1108
HapII CCGG 1 cut(s) 474
HhaI GCGC 3 cut(s) 245, 319, 788
Hin6I GCGC 3 cut(s) 243, 317, 786
HinP1I GCGC 3 cut(s) 243, 317, 786
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HindIII AAGCTT 1 cut(s) 1215
HinfI GANTC 6 cut(s) 772, 814, 832, 858, 1018, 1172
HpaII CCGG 1 cut(s) 474
HphI GGTGA 2 cut(s) 172, 832
Hpy166II GTNNAC 3 cut(s) 67, 254, 634
Hpy188I TCNGA 4 cut(s) 482, 716, 983, 1206
Hpy188III TCNNGA 6 cut(s) 35, 373, 568, 801, 910, 1069
Hpy8I GTNNAC 3 cut(s) 67, 254, 634
HpyAV CCTTC 1 cut(s) 204
HpyCH4III ACNGT 3 cut(s) 71, 114, 661
HpyCH4IV ACGT 1 cut(s) 1134
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 792
HpyF3I CTNAG 4 cut(s) 224, 539, 923, 940
HpySE526I ACGT 1 cut(s) 1134
HspAI GCGC 3 cut(s) 243, 317, 786
Kzo9I GATC 2 cut(s) 728, 943
LguI GCTCTTC 1 cut(s) 1191
LmnI GCTCC 3 cut(s) 127, 485, 698
Lsp1109I GCAGC 2 cut(s) 516, 564
LweI GCATC 5 cut(s) 67, 486, 568, 606, 893
MaeI CTAG 1 cut(s) 1001
MaeII ACGT 1 cut(s) 1134
MaeIII GTNAC 2 cut(s) 178, 583
MalI GATC 2 cut(s) 730, 945
MboI GATC 2 cut(s) 728, 943
MboII GAAGA 3 cut(s) 50, 638, 1208
MfeI CAATTG 1 cut(s) 153
MflI RGATCY 1 cut(s) 943
MlsI TGGCCA 1 cut(s) 1108
MluCI AATT 6 cut(s) 153, 362, 435, 588, 746, 1026
MluNI TGGCCA 1 cut(s) 1108
MlyI GAGTC 4 cut(s) 808, 867, 1012, 1166
MmeI TCCRAC 2 cut(s) 835, 961
MnlI CCTC 9 cut(s) 32, 411, 414, 461, 467, 476, 637, 731, 977
Mox20I TGGCCA 1 cut(s) 1108
Mph1103I ATGCAT 2 cut(s) 561, 1066
MroXI GAANNNNTTC 1 cut(s) 1200
MscI TGGCCA 1 cut(s) 1108
MseI TTAA 3 cut(s) 164, 663, 1219
MslI CAYNNNNRTG 1 cut(s) 1114
Msp20I TGGCCA 1 cut(s) 1108
MspA1I CMGCKG 2 cut(s) 395, 890
MspI CCGG 1 cut(s) 474
MspR9I CCNGG 1 cut(s) 184
MunI CAATTG 1 cut(s) 153
Mva1269I GAATGC 1 cut(s) 1064
MvaI CCWGG 1 cut(s) 184
MvnI CGCG 1 cut(s) 1165
MwoI GCNNNNNNNGC 2 cut(s) 158, 792
NdeII GATC 2 cut(s) 728, 943
NlaIV GGNNCC 3 cut(s) 487, 694, 705
NmuCI GTSAC 2 cut(s) 178, 583
NsbI TGCGCA 1 cut(s) 244
NsiI ATGCAT 2 cut(s) 561, 1066
NspI RCATGY 4 cut(s) 610, 621, 854, 1119
NspV TTCGAA 1 cut(s) 197
PaeI GCATGC 1 cut(s) 621
PaqCI CACCTGC 1 cut(s) 284
PciSI GCTCTTC 1 cut(s) 1191
PctI GAATGC 1 cut(s) 1064
PdmI GAANNNNTTC 1 cut(s) 1200
PfeI GAWTC 2 cut(s) 772, 832
PkrI GCNGC 2 cut(s) 506, 579
PleI GAGTC 4 cut(s) 808, 866, 1012, 1166
PpsI GAGTC 4 cut(s) 808, 866, 1012, 1166
Ppu21I YACGTR 1 cut(s) 1135
Psp6I CCWGG 1 cut(s) 182
PspEI GGTNACC 1 cut(s) 178
PspFI CCCAGC 3 cut(s) 278, 342, 837
PspGI CCWGG 1 cut(s) 182
PspN4I GGNNCC 3 cut(s) 487, 694, 705
PsuI RGATCY 1 cut(s) 943
PvuII CAGCTG 2 cut(s) 395, 890
RsaI GTAC 2 cut(s) 255, 605
RsaNI GTAC 2 cut(s) 254, 604
RseI CAYNNNNRTG 1 cut(s) 1114
SapI GCTCTTC 1 cut(s) 1191
SaqAI TTAA 3 cut(s) 164, 663, 1219
SatI GCNGC 2 cut(s) 505, 578
Sau3AI GATC 2 cut(s) 728, 943
SchI GAGTC 4 cut(s) 808, 867, 1012, 1166
ScrFI CCNGG 1 cut(s) 184
SfaNI GCATC 5 cut(s) 67, 486, 568, 606, 893
SfcI CTRYAG 3 cut(s) 9, 110, 390
SfuI TTCGAA 1 cut(s) 197
SmiMI CAYNNNNRTG 1 cut(s) 1114
SnaBI TACGTA 1 cut(s) 1135
SphI GCATGC 1 cut(s) 621
Sse9I AATT 6 cut(s) 153, 362, 435, 588, 746, 1026
SsiI CCGC 2 cut(s) 56, 621
SspMI CTAG 1 cut(s) 1001
StyD4I CCNGG 1 cut(s) 182
TaaI ACNGT 3 cut(s) 71, 114, 661
TaiI ACGT 1 cut(s) 1137
TaqI TCGA 4 cut(s) 197, 360, 812, 936
TasI AATT 6 cut(s) 153, 362, 435, 588, 746, 1026
TatI WGTACW 2 cut(s) 253, 603
TfiI GAWTC 2 cut(s) 772, 832
Tru1I TTAA 3 cut(s) 164, 663, 1219
Tru9I TTAA 3 cut(s) 164, 663, 1219
TscAI CASTG 3 cut(s) 132, 496, 1012
TseFI GTSAC 2 cut(s) 178, 583
TseI GCWGC 2 cut(s) 504, 577
Tsp45I GTSAC 2 cut(s) 178, 583
TspDTI ATGAA 4 cut(s) 758, 771, 1019, 1072
TspRI CASTG 3 cut(s) 132, 496, 1012
XapI RAATTY 1 cut(s) 362
XceI RCATGY 4 cut(s) 610, 621, 854, 1119
XmnI GAANNNNTTC 1 cut(s) 1200
XspI CTAG 1 cut(s) 1001
Zsp2I ATGCAT 2 cut(s) 561, 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.