RLG00000022572

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0004978
Physical Location & Seq
Reverse (-)
4071402 .. 4072726
1325 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022572

Sequence Viewer

Length: 1227 bp
ATGAGGCTCTTCACATTTCTTTTCTCCTTGCACCTAGTCTTGATCGCCTTCTTGGTAATCTTTGTGACAGTTTACGGTCTCATTTCCACCAATCACAAGCACCATTTCCACCCCCTACAGTTCTATTCTCCACTGCTCACTTCAACACTCTGCTCTGCAATTGTCGCTTTAATATGGCAATGGGTCACCAGGTTAAGCCCTTCGAAAGCCATTAAAACAGCCTTTTGGCTCAGCCCCTTGCTAACCTGCGCAGTTGGTGTACTACTCATATCTATAAGTTCTGCTGGGAGTTTGGCTGCAGGTGGGGTTGCAATCATCTGTGCGCTCATTCAATCGCTGTATGCCTGCTGGGTCAGTCCCCGTATCGAATTTGCCATCAGGATATTATCAGTTTCTACAGCTGTCCCTCCTCCCAAAGTCACAACATTACTCGTTCTGTCAATTTTCACCTGTATCCTCTACCTCTCTTTCTTGGTATCCGGCATTGGAGGAGCCACTGCCATAAGAAGCAGCCTTAGTGTCCTGTTTATCTCGGTTATCTTGCTAAGCATGGCATGGACTATGCATCTCATCAAGAACCTGCTGCAAGTCACAATTTCACGCATCAAGTACATGCACTTTGCATGTGGAGAAGATGTTGACACAAGTGAGGCTTTCTGCAACACACTTAAGCACTCAATGGGGAGTATCTGCCTTGGCTCCATCTTGGTTCCAGTTCTTGGGGTTATTCGGGGCTCAGCCAGAGGCATGAAATTAGTTGCAGGGGATACAGACGAGTTTTTATTTTCATGTGCCAACTGCTACTCAGGACTGGCTTCAACTCTGGTAATGTATGGGAACCGCTGGGGTTTTGTGCACGTTGGAGTCTATAACAAGGGATTTGTGCAGGCATCAGCTGATACATGGGAGATGTTCAGGAATATAGAACTAAAAGAAATAATTGACTTAGATCTCACGACATCATTCTGTTTTCTTTCTGGGGTGGTCGGAGGTGCAATATGTAGCCTAGTCAGTGGAACTTGGACTCTTGCAATTCATAAGAGTTATGCTACTGAAGTCTCCGTATATGCATTCTTGATTGGCTATTTCATGTGCAGGACTGCTATGGCATGGCCACAAGCATGTGTTTCGTCTTACTACGTAGCTTATGCAGAGAACCCTAATAGCACGCGATTCGACTCAACAATTCCTGTTCGCATTGAAGAGCTTCGGAGATTTCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

409

Amino Acids

44.73

Weight (kDa)

8.72

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 103 - 387 3.4e-28 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 290
Acc16I TGCGCA 1 cut(s) 250
Acc36I ACCTGC 3 cut(s) 254, 290, 588
AccB7I CCANNNNNTGG 1 cut(s) 719
AccII CGCG 1 cut(s) 1171
AciI CCGC 1 cut(s) 841
AcoI YGGCCR 1 cut(s) 1112
AcsI RAATTY 1 cut(s) 368
AcuI CTGAAG 1 cut(s) 1074
AfaI GTAC 2 cut(s) 261, 611
AfiI CCNNNNNNNGG 1 cut(s) 719
AflII CTTAAG 1 cut(s) 668
AgsI TTSAA 5 cut(s) 144, 332, 819, 1202, 1220
AjnI CCWGG 1 cut(s) 188
AluBI AGCT 5 cut(s) 401, 896, 1145, 1207, 1223
AluI AGCT 5 cut(s) 401, 896, 1145, 1207, 1223
Alw21I GWGCWC 1 cut(s) 858
Alw26I GTCTC 2 cut(s) 83, 1063
Alw44I GTGCAC 1 cut(s) 854
AoxI GGCC 1 cut(s) 1112
ApaLI GTGCAC 1 cut(s) 854
ApeKI GCWGC 3 cut(s) 296, 510, 583
ApoI RAATTY 1 cut(s) 368
Asp700I GAANNNNTTC 1 cut(s) 1206
AspLEI GCGC 2 cut(s) 251, 325
AsuHPI GGTGA 2 cut(s) 178, 439
AsuII TTCGAA 1 cut(s) 203
BaeGI GKGCMC 1 cut(s) 858
BalI TGGCCA 1 cut(s) 1114
BanII GRGCYC 1 cut(s) 737
Bbv12I GWGCWC 1 cut(s) 858
BbvI GCAGC 3 cut(s) 283, 522, 570
BccI CCATC 2 cut(s) 383, 710
BcgI CGANNNNNNTGC 4 cut(s) 1110, 1144, 1156, 1190
BciT130I CCWGG 1 cut(s) 190
BciVI GTATCC 3 cut(s) 464, 487, 760
BcoDI GTCTC 2 cut(s) 83, 1063
BfaI CTAG 2 cut(s) 35, 1007
BfmI CTRYAG 3 cut(s) 116, 297, 396
BfrI CTTAAG 1 cut(s) 668
BfuAI ACCTGC 3 cut(s) 254, 290, 588
BfuI GTATCC 3 cut(s) 464, 487, 760
BglII AGATCT 1 cut(s) 949
BisI GCNGC 3 cut(s) 297, 511, 584
BlpI GCTNAGC 3 cut(s) 230, 545, 736
BlsI GCNGC 3 cut(s) 298, 512, 585
Bme1390I CCNGG 1 cut(s) 190
BmiI GGNNCC 4 cut(s) 493, 700, 711, 839
BmrFI CCNGG 1 cut(s) 190
BmsI GCATC 3 cut(s) 574, 612, 899
Bpu1102I GCTNAGC 3 cut(s) 230, 545, 736
Bpu14I TTCGAA 1 cut(s) 203
BsaAI YACGTR 1 cut(s) 1141
BsaI GGTCTC 1 cut(s) 83
BsaJI CCNNGG 1 cut(s) 694
BsaXI ACNNNNNCTCC 2 cut(s) 621, 651
Bsc4I CCNNNNNNNGG 1 cut(s) 719
Bse1I ACTGG 2 cut(s) 713, 816
Bse3DI GCAATG 1 cut(s) 185
BseBI CCWGG 1 cut(s) 190
BseDI CCNNGG 1 cut(s) 694
BseLI CCNNNNNNNGG 1 cut(s) 719
BseMI GCAATG 1 cut(s) 185
BseMII CTCAG 3 cut(s) 244, 750, 819
BseNI ACTGG 2 cut(s) 713, 816
BseRI GAGGAG 2 cut(s) 399, 504
BseSI GKGCMC 1 cut(s) 858
BseXI GCAGC 3 cut(s) 283, 522, 570
BseYI CCCAGC 3 cut(s) 284, 348, 843
BsgI GTGCAG 2 cut(s) 905, 1114
Bsh1236I CGCG 1 cut(s) 1171
BshFI GGCC 1 cut(s) 1114
BsiHKAI GWGCWC 1 cut(s) 858
BsiSI CCGG 1 cut(s) 480
BslFI GGGAC 2 cut(s) 342, 389
BslI CCNNNNNNNGG 1 cut(s) 719
BsmAI GTCTC 2 cut(s) 83, 1063
BsmFI GGGAC 2 cut(s) 342, 389
BsmI GAATGC 1 cut(s) 1070
BsnI GGCC 1 cut(s) 1114
Bso31I GGTCTC 1 cut(s) 83
Bsp119I TTCGAA 1 cut(s) 203
Bsp1286I GDGCHC 2 cut(s) 737, 858
Bsp143I GATC 2 cut(s) 42, 949
Bsp1720I GCTNAGC 3 cut(s) 230, 545, 736
BspACI CCGC 1 cut(s) 841
BspANI GGCC 1 cut(s) 1114
BspCNI CTCAG 3 cut(s) 243, 749, 818
BspFNI CGCG 1 cut(s) 1171
BspLI GGNNCC 4 cut(s) 493, 700, 711, 839
BspMAI CTGCAG 1 cut(s) 301
BspMI ACCTGC 3 cut(s) 254, 290, 588
BspQI GCTCTTC 2 cut(s) 14, 1197
BspT104I TTCGAA 1 cut(s) 203
BspTI CTTAAG 1 cut(s) 668
BspTNI GGTCTC 1 cut(s) 83
BsrDI GCAATG 1 cut(s) 185
BsrI ACTGG 2 cut(s) 713, 816
BssECI CCNNGG 1 cut(s) 694
BssMI GATC 2 cut(s) 42, 949
BssT1I CCWWGG 1 cut(s) 694
Bst2UI CCWGG 1 cut(s) 190
Bst4CI ACNGT 3 cut(s) 70, 77, 120
Bst6I CTCTTC 2 cut(s) 14, 1197
BstAFI CTTAAG 1 cut(s) 668
BstBAI YACGTR 1 cut(s) 1141
BstBI TTCGAA 1 cut(s) 203
BstC8I GCNNGC 3 cut(s) 346, 888, 1169
BstDEI CTNAG 6 cut(s) 230, 515, 545, 736, 805, 946
BstEII GGTNACC 1 cut(s) 184
BstFNI CGCG 1 cut(s) 1171
BstHHI GCGC 2 cut(s) 251, 325
BstKTI GATC 2 cut(s) 45, 952
BstMAI GTCTC 2 cut(s) 83, 1063
BstMBI GATC 2 cut(s) 42, 949
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstNI CCWGG 1 cut(s) 190
BstNSI RCATGY 3 cut(s) 616, 627, 1125
BstPI GGTNACC 1 cut(s) 184
BstSCI CCNGG 1 cut(s) 188
BstSFI CTRYAG 3 cut(s) 116, 297, 396
BstSLI GKGCMC 1 cut(s) 858
BstSNI TACGTA 1 cut(s) 1141
BstUI CGCG 1 cut(s) 1171
BstV1I GCAGC 3 cut(s) 283, 522, 570
BstX2I RGATCY 1 cut(s) 949
BstYI RGATCY 1 cut(s) 949
BsuI GTATCC 3 cut(s) 464, 487, 760
BsuRI GGCC 1 cut(s) 1114
BtsI GCAGTG 2 cut(s) 131, 495
BtsIMutI CAGTG 3 cut(s) 131, 495, 1018
BveI ACCTGC 3 cut(s) 254, 290, 588
Cac8I GCNNGC 3 cut(s) 346, 888, 1169
CfoI GCGC 2 cut(s) 251, 325
CsiI ACCWGGT 1 cut(s) 188
Csp6I GTAC 2 cut(s) 260, 610
CviQI GTAC 2 cut(s) 260, 610
DdeI CTNAG 6 cut(s) 230, 515, 545, 736, 805, 946
DpnI GATC 2 cut(s) 44, 951
DpnII GATC 2 cut(s) 42, 949
EaeI YGGCCR 1 cut(s) 1112
Eam1104I CTCTTC 2 cut(s) 14, 1197
EarI CTCTTC 2 cut(s) 14, 1197
Eco105I TACGTA 1 cut(s) 1141
Eco130I CCWWGG 1 cut(s) 694
Eco24I GRGCYC 1 cut(s) 737
Eco31I GGTCTC 1 cut(s) 83
Eco57I CTGAAG 1 cut(s) 1074
Eco91I GGTNACC 1 cut(s) 184
EcoO65I GGTNACC 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 188
EcoT14I CCWWGG 1 cut(s) 694
EcoT22I ATGCAT 2 cut(s) 567, 1072
EcoT38I GRGCYC 1 cut(s) 737
ErhI CCWWGG 1 cut(s) 694
FalI AAGNNNNNCTT 2 cut(s) 637, 669
FaqI GGGAC 2 cut(s) 342, 389
Fnu4HI GCNGC 3 cut(s) 297, 511, 584
FriOI GRGCYC 1 cut(s) 737
Fsp4HI GCNGC 3 cut(s) 297, 511, 584
FspBI CTAG 2 cut(s) 35, 1007
FspI TGCGCA 1 cut(s) 250
GlaI GCGC 2 cut(s) 250, 324
GluI GCNGC 3 cut(s) 297, 511, 584
GsaI CCCAGC 3 cut(s) 288, 352, 847
HaeIII GGCC 1 cut(s) 1114
HapII CCGG 1 cut(s) 480
HhaI GCGC 2 cut(s) 251, 325
Hin6I GCGC 2 cut(s) 249, 323
HinP1I GCGC 2 cut(s) 249, 323
HincII GTYRAC 1 cut(s) 640
HindII GTYRAC 1 cut(s) 640
HindIII AAGCTT 1 cut(s) 1221
HinfI GANTC 4 cut(s) 864, 1024, 1173, 1178
HpaII CCGG 1 cut(s) 480
HphI GGTGA 2 cut(s) 178, 439
Hpy166II GTNNAC 4 cut(s) 73, 260, 640, 856
Hpy188I TCNGA 2 cut(s) 989, 1212
Hpy188III TCNNGA 7 cut(s) 40, 379, 574, 807, 916, 955, 1075
Hpy8I GTNNAC 4 cut(s) 73, 260, 640, 856
HpyAV CCTTC 2 cut(s) 58, 210
HpyCH4III ACNGT 3 cut(s) 70, 77, 120
HpyCH4IV ACGT 2 cut(s) 858, 1140
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
HpyF3I CTNAG 6 cut(s) 230, 515, 545, 736, 805, 946
HpySE526I ACGT 2 cut(s) 858, 1140
HspAI GCGC 2 cut(s) 249, 323
Kzo9I GATC 2 cut(s) 42, 949
LguI GCTCTTC 2 cut(s) 14, 1197
LmnI GCTCC 2 cut(s) 491, 704
Lsp1109I GCAGC 3 cut(s) 283, 522, 570
LweI GCATC 3 cut(s) 574, 612, 899
MabI ACCWGGT 1 cut(s) 188
MaeI CTAG 2 cut(s) 35, 1007
MaeII ACGT 2 cut(s) 858, 1140
MaeIII GTNAC 4 cut(s) 64, 184, 418, 589
MalI GATC 2 cut(s) 44, 951
MboI GATC 2 cut(s) 42, 949
MboII GAAGA 2 cut(s) 644, 1214
MfeI CAATTG 1 cut(s) 159
MflI RGATCY 1 cut(s) 949
MhlI GDGCHC 2 cut(s) 737, 858
MlsI TGGCCA 1 cut(s) 1114
MluCI AATT 8 cut(s) 159, 368, 441, 594, 752, 939, 1032, 1185
MluNI TGGCCA 1 cut(s) 1114
MlyI GAGTC 3 cut(s) 873, 1018, 1172
MmeI TCCRAC 2 cut(s) 841, 967
MnlI CCTC 8 cut(s) 417, 420, 467, 473, 482, 643, 737, 983
Mox20I TGGCCA 1 cut(s) 1114
Mph1103I ATGCAT 2 cut(s) 567, 1072
MroXI GAANNNNTTC 1 cut(s) 1206
MscI TGGCCA 1 cut(s) 1114
MseI TTAA 5 cut(s) 170, 194, 213, 669, 1225
MslI CAYNNNNRTG 1 cut(s) 1120
Msp20I TGGCCA 1 cut(s) 1114
MspA1I CMGCKG 3 cut(s) 401, 843, 896
MspCI CTTAAG 1 cut(s) 668
MspI CCGG 1 cut(s) 480
MspR9I CCNGG 1 cut(s) 190
MunI CAATTG 1 cut(s) 159
Mva1269I GAATGC 1 cut(s) 1070
MvaI CCWGG 1 cut(s) 190
MvnI CGCG 1 cut(s) 1171
MwoI GCNNNNNNNGC 1 cut(s) 164
NdeII GATC 2 cut(s) 42, 949
NlaIV GGNNCC 4 cut(s) 493, 700, 711, 839
NmuCI GTSAC 4 cut(s) 64, 184, 418, 589
NsbI TGCGCA 1 cut(s) 250
NsiI ATGCAT 2 cut(s) 567, 1072
NspI RCATGY 3 cut(s) 616, 627, 1125
NspV TTCGAA 1 cut(s) 203
PaqCI CACCTGC 1 cut(s) 290
PciSI GCTCTTC 2 cut(s) 14, 1197
PctI GAATGC 1 cut(s) 1070
PdmI GAANNNNTTC 1 cut(s) 1206
PfeI GAWTC 1 cut(s) 1173
PflMI CCANNNNNTGG 1 cut(s) 719
PkrI GCNGC 3 cut(s) 298, 512, 585
PleI GAGTC 3 cut(s) 872, 1018, 1172
PpsI GAGTC 3 cut(s) 872, 1018, 1172
Ppu21I YACGTR 1 cut(s) 1141
Psp6I CCWGG 1 cut(s) 188
PspEI GGTNACC 1 cut(s) 184
PspFI CCCAGC 3 cut(s) 284, 348, 843
PspGI CCWGG 1 cut(s) 188
PspN4I GGNNCC 4 cut(s) 493, 700, 711, 839
PstI CTGCAG 1 cut(s) 301
PsuI RGATCY 1 cut(s) 949
PvuII CAGCTG 2 cut(s) 401, 896
RsaI GTAC 2 cut(s) 261, 611
RsaNI GTAC 2 cut(s) 260, 610
RseI CAYNNNNRTG 1 cut(s) 1120
SapI GCTCTTC 2 cut(s) 14, 1197
SaqAI TTAA 5 cut(s) 170, 194, 213, 669, 1225
SatI GCNGC 3 cut(s) 297, 511, 584
Sau3AI GATC 2 cut(s) 42, 949
SchI GAGTC 3 cut(s) 873, 1018, 1172
ScrFI CCNGG 1 cut(s) 190
SduI GDGCHC 2 cut(s) 737, 858
SexAI ACCWGGT 1 cut(s) 188
SfaNI GCATC 3 cut(s) 574, 612, 899
SfcI CTRYAG 3 cut(s) 116, 297, 396
SfuI TTCGAA 1 cut(s) 203
SmiMI CAYNNNNRTG 1 cut(s) 1120
SmlI CTYRAG 1 cut(s) 668
SmoI CTYRAG 1 cut(s) 668
SnaBI TACGTA 1 cut(s) 1141
Sse9I AATT 8 cut(s) 159, 368, 441, 594, 752, 939, 1032, 1185
SsiI CCGC 1 cut(s) 841
SspMI CTAG 2 cut(s) 35, 1007
StyD4I CCNGG 1 cut(s) 188
StyI CCWWGG 1 cut(s) 694
TaaI ACNGT 3 cut(s) 70, 77, 120
TaiI ACGT 2 cut(s) 861, 1143
TaqI TCGA 3 cut(s) 203, 366, 1176
TasI AATT 8 cut(s) 159, 368, 441, 594, 752, 939, 1032, 1185
TatI WGTACW 2 cut(s) 259, 609
TfiI GAWTC 1 cut(s) 1173
Tru1I TTAA 5 cut(s) 170, 194, 213, 669, 1225
Tru9I TTAA 5 cut(s) 170, 194, 213, 669, 1225
TscAI CASTG 3 cut(s) 138, 502, 1018
TseFI GTSAC 4 cut(s) 64, 184, 418, 589
TseI GCWGC 3 cut(s) 296, 510, 583
Tsp45I GTSAC 4 cut(s) 64, 184, 418, 589
TspDTI ATGAA 4 cut(s) 764, 777, 1025, 1078
TspGWI ACGGA 1 cut(s) 1051
TspRI CASTG 3 cut(s) 138, 502, 1018
Van91I CCANNNNNTGG 1 cut(s) 719
Vha464I CTTAAG 1 cut(s) 668
VneI GTGCAC 1 cut(s) 854
XapI RAATTY 1 cut(s) 368
XceI RCATGY 3 cut(s) 616, 627, 1125
XmnI GAANNNNTTC 1 cut(s) 1206
XspI CTAG 2 cut(s) 35, 1007
Zsp2I ATGCAT 2 cut(s) 567, 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.