Rroxscaffold_6G00389650

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004978
Physical Location & Seq
Reverse (-)
3613916 .. 3615757
1842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00389650.1

Sequence Viewer

Length: 1044 bp
ATGGCTGACTTTTCGTCCATCTCTATGGAAAGCATCATACGCCCCCATCAGAGAACTCTTGATCGCCGTCTTGATAATCTTTCTGACAGTTTACGGTCTCATTTCCACCAATCACAAGCACCATTTCCACCCACTACAGTTCTATGCTCCACTGCTCACTTCAACACTCTGCTCTGCAATTGTCGCTTTAATATGGCAATGGGTCACCAGGTGGGGTTGCAATCATCTGTGCGCTCATTCAATCGCTGTATGCCTGCTGGGTCAGTCCCCGTATCGAATTTGCCATCAGGATATTATCAGTTTCTACAGCTGTCCCTCCCCCAAAATCACAACATTACTCGTTCTATCAATTTTCACCTTATGGCATGGACTATGCATCTGATCAAGAACCTGCTGCAAGTCACAATTTCACGCATCAAGTACATGCACTTTGCATGCGGAGAAGATGTTGACACAAGTGAGGCTTTCTGCAACACACTTAAGCACTCAATGGGGAGTATCTGCATTGGCTCCATCTTGGTTCCAGTTCTCGGAGTTATTCGGGGATCAGCCAGAGGCATGAAATTGGTTGCAGGGGATACAGACGAGTTTCTATTTTCATGCGCCAACTGCTATTCAGGACTGGCTTCGACTCTGGTAGTGTATGGGAATCGCTGGGGTTTTGTGCATGTTGGAGTCTATAACAAGGGCTTTGTGCAGGCATCAGCTGATACATGGGAGATGTTCAGGAATATAGAACTAAGAGAAATAATCGACTTGGATCTCACTACATCGTTCTGTTTTCTTTCTGGAGTGGTCGGAGGTGCAATATGTAGCCTGGTCAGCGGAACTTGGACTCTTGCAATTCATAAGAGTTATGCTACTGAAGTCTCCTTATATGCATTCTTGATTGGCTATTTCATGTGCAGGATTGCTACGGCATGGCCACAAGCATGTGTTTTGTCTTACTACGTAGCTTATGCAGAGAACCCTAATAGCACGCGATTTGACTCAACAATTCCTGTTCGCATTGAAGAGCTTCAGAGATTTCAAGCTCAACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

347

Amino Acids

38.8

Weight (kDa)

8.22

Isoelectric Point (pI)

50.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 102 - 324 2.3e-26 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 399
AccII CGCG 1 cut(s) 982
AciI CCGC 2 cut(s) 438, 825
AclWI GGATC 2 cut(s) 553, 768
AcoI YGGCCR 1 cut(s) 923
AcsI RAATTY 1 cut(s) 277
AcuI CTGAAG 2 cut(s) 885, 1004
AdeI CACNNNGTG 1 cut(s) 211
AfaI GTAC 1 cut(s) 422
AfiI CCNNNNNNNGG 1 cut(s) 530
AflII CTTAAG 1 cut(s) 479
AgsI TTSAA 4 cut(s) 163, 241, 1013, 1031
AhdI GACNNNNNGTC 1 cut(s) 13
AjnI CCWGG 2 cut(s) 207, 816
AluBI AGCT 5 cut(s) 310, 707, 956, 1018, 1034
AluI AGCT 5 cut(s) 310, 707, 956, 1018, 1034
Alw26I GTCTC 2 cut(s) 102, 874
AlwI GGATC 2 cut(s) 553, 768
AoxI GGCC 1 cut(s) 923
ApeKI GCWGC 1 cut(s) 394
ApoI RAATTY 1 cut(s) 277
Asp700I GAANNNNTTC 1 cut(s) 1017
AspLEI GCGC 2 cut(s) 234, 605
AsuHPI GGTGA 2 cut(s) 197, 347
BalI TGGCCA 1 cut(s) 925
BbvI GCAGC 1 cut(s) 381
BccI CCATC 4 cut(s) 26, 54, 292, 521
BceAI ACGGC 2 cut(s) 51, 933
BciT130I CCWGG 2 cut(s) 209, 818
BciVI GTATCC 1 cut(s) 571
BclI TGATCA 1 cut(s) 381
BcoDI GTCTC 2 cut(s) 102, 874
BfmI CTRYAG 2 cut(s) 135, 305
BfrI CTTAAG 1 cut(s) 479
BfuAI ACCTGC 1 cut(s) 399
BfuI GTATCC 1 cut(s) 571
BisI GCNGC 1 cut(s) 395
BlsI GCNGC 1 cut(s) 396
Bme1390I CCNGG 2 cut(s) 209, 818
BmeRI GACNNNNNGTC 1 cut(s) 13
BmiI GGNNCC 2 cut(s) 511, 522
BmrFI CCNGG 2 cut(s) 209, 818
BmsI GCATC 4 cut(s) 42, 385, 423, 710
BpmI CTGGAG 1 cut(s) 810
BsaAI YACGTR 1 cut(s) 952
BsaI GGTCTC 1 cut(s) 102
BsaXI ACNNNNNCTCC 2 cut(s) 432, 462
Bsc4I CCNNNNNNNGG 1 cut(s) 530
Bse1I ACTGG 2 cut(s) 524, 627
Bse3DI GCAATG 1 cut(s) 204
BseBI CCWGG 2 cut(s) 209, 818
BseLI CCNNNNNNNGG 1 cut(s) 530
BseMI GCAATG 1 cut(s) 204
BseNI ACTGG 2 cut(s) 524, 627
BseXI GCAGC 1 cut(s) 381
BseYI CCCAGC 2 cut(s) 257, 654
BsgI GTGCAG 2 cut(s) 716, 925
Bsh1236I CGCG 1 cut(s) 982
BshFI GGCC 1 cut(s) 925
BslFI GGGAC 2 cut(s) 251, 298
BslI CCNNNNNNNGG 1 cut(s) 530
BsmAI GTCTC 2 cut(s) 102, 874
BsmFI GGGAC 2 cut(s) 251, 298
BsmI GAATGC 1 cut(s) 881
BsnI GGCC 1 cut(s) 925
Bso31I GGTCTC 1 cut(s) 102
Bsp143I GATC 4 cut(s) 61, 381, 545, 760
BspACI CCGC 2 cut(s) 438, 825
BspANI GGCC 1 cut(s) 925
BspFNI CGCG 1 cut(s) 982
BspLI GGNNCC 2 cut(s) 511, 522
BspMI ACCTGC 1 cut(s) 399
BspPI GGATC 2 cut(s) 553, 768
BspQI GCTCTTC 1 cut(s) 1008
BspTI CTTAAG 1 cut(s) 479
BspTNI GGTCTC 1 cut(s) 102
BsrDI GCAATG 1 cut(s) 204
BsrI ACTGG 2 cut(s) 524, 627
BssMI GATC 4 cut(s) 61, 381, 545, 760
Bst2UI CCWGG 2 cut(s) 209, 818
Bst4CI ACNGT 3 cut(s) 89, 96, 139
Bst6I CTCTTC 1 cut(s) 1008
BstAFI CTTAAG 1 cut(s) 479
BstBAI YACGTR 1 cut(s) 952
BstC8I GCNNGC 4 cut(s) 255, 436, 699, 980
BstDEI CTNAG 1 cut(s) 740
BstEII GGTNACC 1 cut(s) 203
BstFNI CGCG 1 cut(s) 982
BstHHI GCGC 2 cut(s) 234, 605
BstKTI GATC 4 cut(s) 64, 384, 548, 763
BstMAI GTCTC 2 cut(s) 102, 874
BstMBI GATC 4 cut(s) 61, 381, 545, 760
BstMWI GCNNNNNNNGC 4 cut(s) 39, 183, 609, 822
BstNI CCWGG 2 cut(s) 209, 818
BstNSI RCATGY 4 cut(s) 427, 438, 671, 936
BstPI GGTNACC 1 cut(s) 203
BstSCI CCNGG 2 cut(s) 207, 816
BstSFI CTRYAG 2 cut(s) 135, 305
BstSNI TACGTA 1 cut(s) 952
BstUI CGCG 1 cut(s) 982
BstV1I GCAGC 1 cut(s) 381
BstX2I RGATCY 1 cut(s) 760
BstXI CCANNNNNNTGG 1 cut(s) 25
BstYI RGATCY 1 cut(s) 760
BsuI GTATCC 1 cut(s) 571
BsuRI GGCC 1 cut(s) 925
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 1 cut(s) 150
BveI ACCTGC 1 cut(s) 399
Cac8I GCNNGC 4 cut(s) 255, 436, 699, 980
CfoI GCGC 2 cut(s) 234, 605
CsiI ACCWGGT 1 cut(s) 207
Csp6I GTAC 1 cut(s) 421
CviQI GTAC 1 cut(s) 421
DdeI CTNAG 1 cut(s) 740
DpnI GATC 4 cut(s) 63, 383, 547, 762
DpnII GATC 4 cut(s) 61, 381, 545, 760
DraIII CACNNNGTG 1 cut(s) 211
DriI GACNNNNNGTC 1 cut(s) 13
EaeI YGGCCR 1 cut(s) 923
Eam1104I CTCTTC 1 cut(s) 1008
Eam1105I GACNNNNNGTC 1 cut(s) 13
EarI CTCTTC 1 cut(s) 1008
Eco105I TACGTA 1 cut(s) 952
Eco31I GGTCTC 1 cut(s) 102
Eco57I CTGAAG 2 cut(s) 885, 1004
Eco91I GGTNACC 1 cut(s) 203
EcoO65I GGTNACC 1 cut(s) 203
EcoRII CCWGG 2 cut(s) 207, 816
EcoT22I ATGCAT 2 cut(s) 378, 883
FalI AAGNNNNNCTT 2 cut(s) 448, 480
FaqI GGGAC 2 cut(s) 251, 298
FbaI TGATCA 1 cut(s) 381
Fnu4HI GCNGC 1 cut(s) 395
Fsp4HI GCNGC 1 cut(s) 395
GlaI GCGC 2 cut(s) 233, 604
GluI GCNGC 1 cut(s) 395
GsaI CCCAGC 2 cut(s) 261, 658
GsuI CTGGAG 1 cut(s) 810
HaeIII GGCC 1 cut(s) 925
HhaI GCGC 2 cut(s) 234, 605
Hin6I GCGC 2 cut(s) 232, 603
HinP1I GCGC 2 cut(s) 232, 603
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HinfI GANTC 5 cut(s) 631, 649, 675, 835, 989
HphI GGTGA 2 cut(s) 197, 347
Hpy166II GTNNAC 2 cut(s) 92, 451
Hpy188I TCNGA 6 cut(s) 51, 85, 381, 533, 800, 1023
Hpy188III TCNNGA 8 cut(s) 59, 71, 288, 385, 618, 727, 789, 886
Hpy8I GTNNAC 2 cut(s) 92, 451
HpyCH4III ACNGT 3 cut(s) 89, 96, 139
HpyCH4IV ACGT 1 cut(s) 951
HpyF10VI GCNNNNNNNGC 4 cut(s) 39, 183, 609, 822
HpyF3I CTNAG 1 cut(s) 740
HpySE526I ACGT 1 cut(s) 951
HspAI GCGC 2 cut(s) 232, 603
Ksp22I TGATCA 1 cut(s) 381
Kzo9I GATC 4 cut(s) 61, 381, 545, 760
LguI GCTCTTC 1 cut(s) 1008
LmnI GCTCC 2 cut(s) 152, 515
Lsp1109I GCAGC 1 cut(s) 381
LweI GCATC 4 cut(s) 42, 385, 423, 710
MabI ACCWGGT 1 cut(s) 207
MaeII ACGT 1 cut(s) 951
MaeIII GTNAC 2 cut(s) 203, 400
MalI GATC 4 cut(s) 63, 383, 547, 762
MboI GATC 4 cut(s) 61, 381, 545, 760
MboII GAAGA 2 cut(s) 455, 1025
MfeI CAATTG 1 cut(s) 178
MflI RGATCY 1 cut(s) 760
MlsI TGGCCA 1 cut(s) 925
MluCI AATT 7 cut(s) 178, 277, 349, 405, 563, 843, 996
MluNI TGGCCA 1 cut(s) 925
MlyI GAGTC 4 cut(s) 625, 684, 829, 983
MmeI TCCRAC 2 cut(s) 652, 778
MnlI CCTC 4 cut(s) 326, 454, 548, 794
Mox20I TGGCCA 1 cut(s) 925
Mph1103I ATGCAT 2 cut(s) 378, 883
MroXI GAANNNNTTC 1 cut(s) 1017
MscI TGGCCA 1 cut(s) 925
MseI TTAA 2 cut(s) 189, 480
MslI CAYNNNNRTG 2 cut(s) 23, 931
Msp20I TGGCCA 1 cut(s) 925
MspA1I CMGCKG 3 cut(s) 310, 707, 825
MspCI CTTAAG 1 cut(s) 479
MspR9I CCNGG 2 cut(s) 209, 818
MunI CAATTG 1 cut(s) 178
Mva1269I GAATGC 1 cut(s) 881
MvaI CCWGG 2 cut(s) 209, 818
MvnI CGCG 1 cut(s) 982
MwoI GCNNNNNNNGC 4 cut(s) 39, 183, 609, 822
NdeII GATC 4 cut(s) 61, 381, 545, 760
NlaIV GGNNCC 2 cut(s) 511, 522
NmuCI GTSAC 2 cut(s) 203, 400
NsiI ATGCAT 2 cut(s) 378, 883
NspI RCATGY 4 cut(s) 427, 438, 671, 936
PaeI GCATGC 1 cut(s) 438
PciSI GCTCTTC 1 cut(s) 1008
PctI GAATGC 1 cut(s) 881
PdmI GAANNNNTTC 1 cut(s) 1017
PfeI GAWTC 1 cut(s) 649
PkrI GCNGC 1 cut(s) 396
PleI GAGTC 4 cut(s) 625, 683, 829, 983
PpsI GAGTC 4 cut(s) 625, 683, 829, 983
Ppu21I YACGTR 1 cut(s) 952
Psp6I CCWGG 2 cut(s) 207, 816
PspEI GGTNACC 1 cut(s) 203
PspFI CCCAGC 2 cut(s) 257, 654
PspGI CCWGG 2 cut(s) 207, 816
PspN4I GGNNCC 2 cut(s) 511, 522
PsuI RGATCY 1 cut(s) 760
PvuII CAGCTG 2 cut(s) 310, 707
RsaI GTAC 1 cut(s) 422
RsaNI GTAC 1 cut(s) 421
RseI CAYNNNNRTG 2 cut(s) 23, 931
SapI GCTCTTC 1 cut(s) 1008
SaqAI TTAA 2 cut(s) 189, 480
SatI GCNGC 1 cut(s) 395
Sau3AI GATC 4 cut(s) 61, 381, 545, 760
SchI GAGTC 4 cut(s) 625, 684, 829, 983
ScrFI CCNGG 2 cut(s) 209, 818
SexAI ACCWGGT 1 cut(s) 207
SfaNI GCATC 4 cut(s) 42, 385, 423, 710
SfcI CTRYAG 2 cut(s) 135, 305
SmiMI CAYNNNNRTG 2 cut(s) 23, 931
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
SnaBI TACGTA 1 cut(s) 952
SphI GCATGC 1 cut(s) 438
Sse9I AATT 7 cut(s) 178, 277, 349, 405, 563, 843, 996
SsiI CCGC 2 cut(s) 438, 825
StyD4I CCNGG 2 cut(s) 207, 816
TaaI ACNGT 3 cut(s) 89, 96, 139
TaiI ACGT 1 cut(s) 954
TaqI TCGA 3 cut(s) 275, 629, 753
TasI AATT 7 cut(s) 178, 277, 349, 405, 563, 843, 996
TatI WGTACW 1 cut(s) 420
TfiI GAWTC 1 cut(s) 649
Tru1I TTAA 2 cut(s) 189, 480
Tru9I TTAA 2 cut(s) 189, 480
TscAI CASTG 1 cut(s) 157
TseFI GTSAC 2 cut(s) 203, 400
TseI GCWGC 1 cut(s) 394
Tsp45I GTSAC 2 cut(s) 203, 400
TspDTI ATGAA 4 cut(s) 575, 588, 836, 889
TspRI CASTG 1 cut(s) 157
Vha464I CTTAAG 1 cut(s) 479
XapI RAATTY 1 cut(s) 277
XceI RCATGY 4 cut(s) 427, 438, 671, 936
XmnI GAANNNNTTC 1 cut(s) 1017
Zsp2I ATGCAT 2 cut(s) 378, 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.