MD12G1012000.v1.1

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004978
Physical Location & Seq
Reverse (-)
1225047 .. 1226239
1193 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1012000.v1.1.491

Sequence Viewer

Length: 1173 bp
ATGCAGACGCAAGAACCTAGCAACTTTCCACCCGAAACAACCACAGCCGGACAATTTCTCAGGAGGCTCTTTAAACTTCTTTTTTACTTGCACTTGATCCTCATCACCATCTTCGTAATCTTCCTTACGCTCTATGGCCTCATCTTCAACACTCGCACTCACCATTTCCGACCCCTCGAGTATTACCCTCCATTGCTCACTGCAATTGGGTTCTCTGCAATTTTTGCTTTCATATGGCAGTGGATGACTAGCTCAAGCCCTTCCAAAGCCTTCAAGGCAGCCTTCTGGCTTAGCCCTTTGCTAACATGCGCGGTCGGCGTACTACTTATAGACATAAGTTCTCCTGCGAGTTTGGCTGCAGGTGTGGTTGCAATCGTTTGTGCCGTAATTCAGTCTCTGTACGCCTGTTGGGTCAGTCCCCGTTTTGACTACGCAATTCGAGTTTTATCAGTTGCCACTGCCTTTCCTCCTCCGAAGACTACAATATTAGTCATTCAATCAATTTCCGTCAGCATTCTTTACTCGTGTCTCTTGGTGTCCGGCATTGGAAGAGCCACTGCTACCAGGAGTAGTTGGAAGGCCCTGTTCATCGCACTAATCTTGCTAAGCATGGCATGGACGATGCAAGTAATCAAGAACACGCTGCTAGTTACCGTCTCGCGAATCAAGTTTATGAACTTGGCATTTGGAACAGAGATCGACACACGTGAGGCTTTCCGCGATACCATTAAGCACTTGACTGGGAGTATCTGCATCGGCTCCATCTTGGTCCCGGTTCTTGGGGTTGTTCGTGGCTCAGCCCGAGCTGTGAAACTGACTGCAGGGGATACAGACGAGTTTCTGTTTTCGTGTGCCAACTGCTATTCAGGAATGGCTTCGACGCTGGTGATGTATGGGAATCGCTGGGGTTTTGTGCACGTAGGAGCGTACAATAAGGGGTTTGTGCAGGCATCGTCGGATACTTGGGAGATGTTCGGGGACGAAGGACTGAAAGAATTAATCGACTCGGATCTCACCGGATCGTTCTGTTTTCTTTCTGGGGTGGCAGCAGGTGCAATCTGCAGCCTAGTGAGTGGGACTTGGGCTCTTATAATTCACAGGAGCTATGCAACAGAAGTTTCAATATACGCATTCTTCATCGGATACTTCATGGTACTTATCCTTCCTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

391

Amino Acids

42.86

Weight (kDa)

8.81

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 122 - 385 6.7e-22 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1091
AarI CACCTGC 2 cut(s) 350, 1040
Acc36I ACCTGC 2 cut(s) 350, 1040
AccII CGCG 3 cut(s) 311, 661, 720
AciI CCGC 2 cut(s) 311, 718
AclWI GGATC 3 cut(s) 91, 1017, 1027
AcvI CACGTG 1 cut(s) 707
AfaI GTAC 4 cut(s) 321, 401, 929, 1155
AfiI CCNNNNNNNGG 1 cut(s) 779
AflIII ACRYGT 1 cut(s) 704
AgsI TTSAA 4 cut(s) 148, 274, 497, 1122
AjnI CCWGG 1 cut(s) 563
AluBI AGCT 3 cut(s) 252, 806, 1104
AluI AGCT 3 cut(s) 252, 806, 1104
Alw21I GWGCWC 1 cut(s) 918
Alw26I GTCTC 3 cut(s) 399, 533, 661
Alw44I GTGCAC 1 cut(s) 914
AlwI GGATC 3 cut(s) 91, 1017, 1027
AlwNI CAGNNNCTG 1 cut(s) 397
Ama87I CYCGRG 2 cut(s) 176, 801
AoxI GGCC 2 cut(s) 136, 579
ApaLI GTGCAC 1 cut(s) 914
ApeKI GCWGC 5 cut(s) 278, 356, 643, 1046, 1062
AseI ATTAAT 1 cut(s) 998
Asp700I GAANNNNTTC 1 cut(s) 874
AspLEI GCGC 1 cut(s) 311
AspS9I GGNCC 2 cut(s) 580, 769
AsuC2I CCSGG 1 cut(s) 773
AsuHPI GGTGA 4 cut(s) 97, 152, 898, 1006
AvaI CYCGRG 2 cut(s) 176, 801
AvaII GGWCC 1 cut(s) 769
BaeGI GKGCMC 1 cut(s) 918
BanII GRGCYC 1 cut(s) 1087
BarI GAAGNNNNNNTAC 1 cut(s) 1146
BauI CACGAG 1 cut(s) 523
BbrPI CACGTG 1 cut(s) 707
BbsI GAAGAC 1 cut(s) 482
Bbv12I GWGCWC 1 cut(s) 918
BbvI GCAGC 5 cut(s) 290, 343, 630, 1058, 1074
BccI CCATC 2 cut(s) 116, 770
BceAI ACGGC 1 cut(s) 368
BciT130I CCWGG 1 cut(s) 565
BciVI GTATCC 3 cut(s) 820, 952, 1136
BcnI CCSGG 1 cut(s) 773
BcoDI GTCTC 3 cut(s) 399, 533, 661
BfaI CTAG 4 cut(s) 18, 249, 647, 1067
BfmI CTRYAG 3 cut(s) 357, 819, 1060
BfuAI ACCTGC 2 cut(s) 350, 1040
BfuI GTATCC 3 cut(s) 820, 952, 1136
BglI GCCNNNNNGGC 1 cut(s) 275
BisI GCNGC 5 cut(s) 279, 357, 644, 1047, 1063
BlpI GCTNAGC 3 cut(s) 290, 605, 796
BlsI GCNGC 5 cut(s) 280, 358, 645, 1048, 1064
Bme1390I CCNGG 2 cut(s) 565, 773
Bme18I GGWCC 1 cut(s) 769
BmeT110I CYCGRG 2 cut(s) 176, 801
BmgT120I GGNCC 2 cut(s) 580, 769
BmiI GGNNCC 2 cut(s) 760, 771
BmrFI CCNGG 2 cut(s) 565, 773
BmrI ACTGGG 1 cut(s) 750
BmsI GCATC 3 cut(s) 612, 762, 959
BmuI ACTGGG 1 cut(s) 750
BpiI GAAGAC 1 cut(s) 482
Bpu1102I GCTNAGC 3 cut(s) 290, 605, 796
BpuEI CTTGAG 1 cut(s) 238
BpuMI CCSGG 1 cut(s) 773
BsaAI YACGTR 2 cut(s) 707, 919
BsaBI GATNNNNATC 1 cut(s) 101
BsaWI WCCGGW 1 cut(s) 1016
Bsc4I CCNNNNNNNGG 1 cut(s) 779
Bse1I ACTGG 1 cut(s) 745
Bse3DI GCAATG 1 cut(s) 191
Bse8I GATNNNNATC 1 cut(s) 101
BseBI CCWGG 1 cut(s) 565
BseGI GGATG 1 cut(s) 249
BseJI GATNNNNATC 1 cut(s) 101
BseLI CCNNNNNNNGG 1 cut(s) 779
BseMI GCAATG 1 cut(s) 191
BseMII CTCAG 2 cut(s) 73, 810
BseNI ACTGG 1 cut(s) 745
BseRI GAGGAG 1 cut(s) 459
BseSI GKGCMC 1 cut(s) 918
BseXI GCAGC 5 cut(s) 290, 343, 630, 1058, 1074
BseYI CCCAGC 1 cut(s) 903
BsgI GTGCAG 1 cut(s) 965
Bsh1236I CGCG 3 cut(s) 311, 661, 720
Bsh1285I CGRYCG 1 cut(s) 315
BshFI GGCC 2 cut(s) 138, 581
BsiEI CGRYCG 1 cut(s) 315
BsiHKAI GWGCWC 1 cut(s) 918
BsiHKCI CYCGRG 2 cut(s) 176, 801
BsiSI CCGG 4 cut(s) 48, 540, 773, 1017
BslFI GGGAC 4 cut(s) 402, 755, 992, 1090
BslI CCNNNNNNNGG 1 cut(s) 779
BsmAI GTCTC 3 cut(s) 399, 533, 661
BsmBI CGTCTC 1 cut(s) 661
BsmFI GGGAC 4 cut(s) 402, 755, 992, 1090
BsmI GAATGC 2 cut(s) 513, 1130
BsnI GGCC 2 cut(s) 138, 581
BsoBI CYCGRG 2 cut(s) 176, 801
Bsp1286I GDGCHC 2 cut(s) 918, 1087
Bsp143I GATC 4 cut(s) 96, 696, 1009, 1019
Bsp1720I GCTNAGC 3 cut(s) 290, 605, 796
Bsp68I TCGCGA 1 cut(s) 661
BspACI CCGC 2 cut(s) 311, 718
BspANI GGCC 2 cut(s) 138, 581
BspCNI CTCAG 2 cut(s) 72, 809
BspFNI CGCG 3 cut(s) 311, 661, 720
BspLI GGNNCC 2 cut(s) 760, 771
BspMAI CTGCAG 3 cut(s) 361, 823, 1064
BspMI ACCTGC 2 cut(s) 350, 1040
BspPI GGATC 3 cut(s) 91, 1017, 1027
BspQI GCTCTTC 1 cut(s) 544
BsrDI GCAATG 1 cut(s) 191
BsrI ACTGG 1 cut(s) 745
BssMI GATC 4 cut(s) 96, 696, 1009, 1019
BssSI CACGAG 1 cut(s) 523
Bst2BI CACGAG 1 cut(s) 523
Bst2UI CCWGG 1 cut(s) 565
Bst4CI ACNGT 1 cut(s) 655
Bst6I CTCTTC 1 cut(s) 544
BstAPI GCANNNNNTGC 2 cut(s) 224, 1052
BstBAI YACGTR 2 cut(s) 707, 919
BstC8I GCNNGC 1 cut(s) 948
BstDEI CTNAG 5 cut(s) 59, 290, 605, 796, 1167
BstF5I GGATG 1 cut(s) 249
BstFNI CGCG 3 cut(s) 311, 661, 720
BstHHI GCGC 1 cut(s) 311
BstKTI GATC 4 cut(s) 99, 699, 1012, 1022
BstMAI GTCTC 3 cut(s) 399, 533, 661
BstMBI GATC 4 cut(s) 96, 696, 1009, 1019
BstMCI CGRYCG 1 cut(s) 315
BstMWI GCNNNNNNNGC 5 cut(s) 224, 275, 315, 353, 1052
BstNI CCWGG 1 cut(s) 565
BstNSI RCATGY 1 cut(s) 309
BstSCI CCNGG 2 cut(s) 563, 771
BstSFI CTRYAG 3 cut(s) 357, 819, 1060
BstSLI GKGCMC 1 cut(s) 918
BstUI CGCG 3 cut(s) 311, 661, 720
BstV1I GCAGC 5 cut(s) 290, 343, 630, 1058, 1074
BstV2I GAAGAC 1 cut(s) 482
BstX2I RGATCY 1 cut(s) 1009
BstYI RGATCY 1 cut(s) 1009
BsuI GTATCC 3 cut(s) 820, 952, 1136
BsuRI GGCC 2 cut(s) 138, 581
BtgZI GCGATG 1 cut(s) 574
BtsCI GGATG 1 cut(s) 249
BtsI GCAGTG 4 cut(s) 198, 245, 456, 555
BtsIMutI CAGTG 4 cut(s) 198, 245, 456, 555
BtuMI TCGCGA 1 cut(s) 661
BveI ACCTGC 2 cut(s) 350, 1040
Cac8I GCNNGC 1 cut(s) 948
CaiI CAGNNNCTG 1 cut(s) 397
CfoI GCGC 1 cut(s) 311
Cfr13I GGNCC 2 cut(s) 580, 769
CseI GACGC 2 cut(s) 16, 889
Csp6I GTAC 4 cut(s) 320, 400, 928, 1154
CviAII CATG 4 cut(s) 306, 610, 615, 1150
CviQI GTAC 4 cut(s) 320, 400, 928, 1154
DdeI CTNAG 5 cut(s) 59, 290, 605, 796, 1167
DpnI GATC 4 cut(s) 98, 698, 1011, 1021
DpnII GATC 4 cut(s) 96, 696, 1009, 1019
DraI TTTAAA 1 cut(s) 73
Eam1104I CTCTTC 1 cut(s) 544
EarI CTCTTC 1 cut(s) 544
Eco24I GRGCYC 1 cut(s) 1087
Eco47I GGWCC 1 cut(s) 769
Eco72I CACGTG 1 cut(s) 707
Eco88I CYCGRG 2 cut(s) 176, 801
EcoO109I RGGNCCY 1 cut(s) 580
EcoRII CCWGG 1 cut(s) 563
EcoT38I GRGCYC 1 cut(s) 1087
Esp3I CGTCTC 1 cut(s) 661
FaeI CATG 4 cut(s) 309, 613, 618, 1153
FalI AAGNNNNNCTT 2 cut(s) 266, 298
FaqI GGGAC 4 cut(s) 402, 755, 992, 1090
FatI CATG 4 cut(s) 305, 609, 614, 1149
FauNDI CATATG 1 cut(s) 233
Fnu4HI GCNGC 5 cut(s) 279, 357, 644, 1047, 1063
FokI GGATG 1 cut(s) 256
FriOI GRGCYC 1 cut(s) 1087
Fsp4HI GCNGC 5 cut(s) 279, 357, 644, 1047, 1063
FspBI CTAG 4 cut(s) 18, 249, 647, 1067
GlaI GCGC 1 cut(s) 310
GluI GCNGC 5 cut(s) 279, 357, 644, 1047, 1063
GsaI CCCAGC 1 cut(s) 907
HaeIII GGCC 2 cut(s) 138, 581
HapII CCGG 4 cut(s) 48, 540, 773, 1017
HgaI GACGC 2 cut(s) 16, 889
HhaI GCGC 1 cut(s) 311
Hin1II CATG 4 cut(s) 309, 613, 618, 1153
Hin6I GCGC 1 cut(s) 309
HinP1I GCGC 1 cut(s) 309
HinfI GANTC 3 cut(s) 663, 898, 1004
HpaII CCGG 4 cut(s) 48, 540, 773, 1017
HphI GGTGA 4 cut(s) 97, 152, 898, 1006
Hpy166II GTNNAC 1 cut(s) 916
Hpy188I TCNGA 5 cut(s) 170, 474, 958, 1009, 1142
Hpy188III TCNNGA 4 cut(s) 61, 634, 660, 867
Hpy8I GTNNAC 1 cut(s) 916
Hpy99I CGWCG 2 cut(s) 883, 958
HpyAV CCTTC 6 cut(s) 270, 280, 292, 571, 977, 1172
HpyCH4III ACNGT 1 cut(s) 655
HpyCH4IV ACGT 2 cut(s) 706, 918
HpyF10VI GCNNNNNNNGC 5 cut(s) 224, 275, 315, 353, 1052
HpyF3I CTNAG 5 cut(s) 59, 290, 605, 796, 1167
HpySE526I ACGT 2 cut(s) 706, 918
Hsp92II CATG 4 cut(s) 309, 613, 618, 1153
HspAI GCGC 1 cut(s) 309
Kzo9I GATC 4 cut(s) 96, 696, 1009, 1019
LguI GCTCTTC 1 cut(s) 544
LmnI GCTCC 3 cut(s) 764, 923, 1101
Lsp1109I GCAGC 5 cut(s) 290, 343, 630, 1058, 1074
LweI GCATC 3 cut(s) 612, 762, 959
MaeI CTAG 4 cut(s) 18, 249, 647, 1067
MaeII ACGT 2 cut(s) 706, 918
MaeIII GTNAC 1 cut(s) 649
MalI GATC 4 cut(s) 98, 698, 1011, 1021
MboI GATC 4 cut(s) 96, 696, 1009, 1019
MboII GAAGA 6 cut(s) 103, 112, 136, 487, 561, 1126
MfeI CAATTG 1 cut(s) 204
MflI RGATCY 1 cut(s) 1009
MhlI GDGCHC 2 cut(s) 918, 1087
MluCI AATT 8 cut(s) 53, 204, 219, 387, 435, 501, 995, 1092
MlyI GAGTC 1 cut(s) 998
MmeI TCCRAC 3 cut(s) 193, 554, 936
MnlI CCTC 8 cut(s) 57, 110, 149, 185, 198, 477, 480, 703
MroXI GAANNNNTTC 1 cut(s) 874
MseI TTAA 3 cut(s) 72, 729, 998
MspI CCGG 4 cut(s) 48, 540, 773, 1017
MspR9I CCNGG 2 cut(s) 565, 773
MunI CAATTG 1 cut(s) 204
Mva1269I GAATGC 2 cut(s) 513, 1130
MvaI CCWGG 1 cut(s) 565
MvnI CGCG 3 cut(s) 311, 661, 720
MwoI GCNNNNNNNGC 5 cut(s) 224, 275, 315, 353, 1052
NciI CCSGG 1 cut(s) 773
NdeI CATATG 1 cut(s) 233
NdeII GATC 4 cut(s) 96, 696, 1009, 1019
NlaIII CATG 4 cut(s) 309, 613, 618, 1153
NlaIV GGNNCC 2 cut(s) 760, 771
NruI TCGCGA 1 cut(s) 661
NspI RCATGY 1 cut(s) 309
PaeR7I CTCGAG 1 cut(s) 176
PaqCI CACCTGC 2 cut(s) 350, 1040
PciSI GCTCTTC 1 cut(s) 544
PcsI WCGNNNNNNNCGW 1 cut(s) 381
PctI GAATGC 2 cut(s) 513, 1130
PdmI GAANNNNTTC 1 cut(s) 874
PfeI GAWTC 2 cut(s) 663, 898
PkrI GCNGC 5 cut(s) 280, 358, 645, 1048, 1064
PleI GAGTC 1 cut(s) 998
PmaCI CACGTG 1 cut(s) 707
PmlI CACGTG 1 cut(s) 707
PpsI GAGTC 1 cut(s) 998
Ppu21I YACGTR 2 cut(s) 707, 919
PshBI ATTAAT 1 cut(s) 998
PsiI TTATAA 1 cut(s) 1091
Psp6I CCWGG 1 cut(s) 563
PspCI CACGTG 1 cut(s) 707
PspFI CCCAGC 1 cut(s) 903
PspGI CCWGG 1 cut(s) 563
PspN4I GGNNCC 2 cut(s) 760, 771
PspPI GGNCC 2 cut(s) 580, 769
PspXI VCTCGAGB 1 cut(s) 176
PstI CTGCAG 3 cut(s) 361, 823, 1064
PstNI CAGNNNCTG 1 cut(s) 397
PsuI RGATCY 1 cut(s) 1009
RruI TCGCGA 1 cut(s) 661
RsaI GTAC 4 cut(s) 321, 401, 929, 1155
RsaNI GTAC 4 cut(s) 320, 400, 928, 1154
SapI GCTCTTC 1 cut(s) 544
SaqAI TTAA 3 cut(s) 72, 729, 998
SatI GCNGC 5 cut(s) 279, 357, 644, 1047, 1063
Sau3AI GATC 4 cut(s) 96, 696, 1009, 1019
Sau96I GGNCC 2 cut(s) 580, 769
SchI GAGTC 1 cut(s) 998
ScrFI CCNGG 2 cut(s) 565, 773
SduI GDGCHC 2 cut(s) 918, 1087
SetI ASST 8 cut(s) 19, 254, 364, 709, 808, 921, 1054, 1106
SfaNI GCATC 3 cut(s) 612, 762, 959
SfcI CTRYAG 3 cut(s) 357, 819, 1060
Sfr274I CTCGAG 1 cut(s) 176
SinI GGWCC 1 cut(s) 769
SlaI CTCGAG 1 cut(s) 176
SmlI CTYRAG 2 cut(s) 176, 253
SmoI CTYRAG 2 cut(s) 176, 253
Sse9I AATT 8 cut(s) 53, 204, 219, 387, 435, 501, 995, 1092
SsiI CCGC 2 cut(s) 311, 718
SspI AATATT 1 cut(s) 486
SspMI CTAG 4 cut(s) 18, 249, 647, 1067
StyD4I CCNGG 2 cut(s) 563, 771
TaaI ACNGT 1 cut(s) 655
TaiI ACGT 2 cut(s) 709, 921
TaqI TCGA 5 cut(s) 177, 439, 699, 878, 1002
TasI AATT 8 cut(s) 53, 204, 219, 387, 435, 501, 995, 1092
TfiI GAWTC 2 cut(s) 663, 898
Tru1I TTAA 3 cut(s) 72, 729, 998
Tru9I TTAA 3 cut(s) 72, 729, 998
TscAI CASTG 4 cut(s) 205, 245, 463, 562
TseI GCWGC 5 cut(s) 278, 356, 643, 1046, 1062
TspDTI ATGAA 5 cut(s) 220, 577, 689, 1126, 1138
TspGWI ACGGA 1 cut(s) 496
TspRI CASTG 4 cut(s) 205, 245, 463, 562
VneI GTGCAC 1 cut(s) 914
VpaK11BI GGWCC 1 cut(s) 769
VspI ATTAAT 1 cut(s) 998
XceI RCATGY 1 cut(s) 309
XhoI CTCGAG 1 cut(s) 176
XmnI GAANNNNTTC 1 cut(s) 874
XspI CTAG 4 cut(s) 18, 249, 647, 1067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.