Rh3AG325800

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0004978
Physical Location & Seq
Forward (+)
46169861 .. 46171862
2002 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG325800.1

Sequence Viewer

Length: 1227 bp
ATGAGGCTCTTCACATTTATTTTCTTCTTGCACCTAGTCTTGATCGCCTTCTTGATAATCTTTCTGACAGTTTACGGTCTCATTTCGACCAATCACAAGCACCATTTCCACCCACTAAAGTTCTATGCTCCACTGCTCACTTCAACACTCTGCTCTGCAATTTTCGCTTTAATATGGCAATGGGTCACCAGGTTAAGCCCTTCGAAAGCCATTAAAACTGCCTTTTGGCTCAGCCCCTTGCTAACCTGTGCAGTTGGTGTACTACTCATATCTATAAGTTCTGCTGGGGGTTTGGCTGCAGGTGGGGTTGCAATCATCTGTGCGTTCATTCAATCGCTGTATGCCTGCTGGGTCAGTCCCCGTATCGAATTTGCCATCAGGATATTATCTGTTTCTACAGCTGTCCCTCCTCCCAAAATCACAACATTACTCATTCTATCAATTTTTACCTGTATCCTCTACCTCTCTTTCTTGGTATCTGGCATCGGAGGAGCCACTGCCATAAGAAGCAGCTTTAGTGTCCTGTTTATCTCGGTTATCTTGCTAAGCATGGCATGGACTATGCATCTCATCAAGAACCTGCTGCAAGTCACAATTTCACGCATCAAGTACATGCACTTTGCATGCGGAGAAGATGTTTACACAAGTGAGGCTTTCTACAGCACACTTAAGCACTCAATGGGGAGTATCTGCATTGGCTCCATCTTGGTTCCAGTTCTCGGGGTTATTCGGGGCTCAGCCAGAGGCATGAAATCGGTTGCAGGGGATACAGACGAGTTTCTATTTTCATGTGCCAACTGCTACTCAGGACTGGCTTCAACTCTGGTAATGTATGGGAATCGCTGGGGTTTTGTGCATGTTGGAGTCTATAACAAGGGATTTGTGCAGGCATCAGCTGATACATGGGAGATGTTCAGGAATATAGAACTAAAAGAAATAATCGACTTAGATCTCACTACATCGTTCTGTTTTCTTTCTGGGGTGGTCGGAGGTGCAATATGTAGCCTAGTCAGTGGAACTTGGACTCTTGCAATTCATAAGAGTTATGTTACTGAAGTCTCCTTATATGCATTCTTGATTGGCTATTTCATGTGCAGGATTGCTATGGCATGGCCACAAGCATGTGTTTTGTCTTACTATGTAGCTTATGCAGAGAACCCTAATAGCACGCGATTTGACTCAACAATTCCTGTTCGCATTGAAGAGCTTCGGAGATTTCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

408

Amino Acids

45.05

Weight (kDa)

8.92

Isoelectric Point (pI)

42.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 103 - 387 4.8e-27 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 290
Acc36I ACCTGC 2 cut(s) 290, 588
AccII CGCG 1 cut(s) 1171
AciI CCGC 1 cut(s) 627
AcoI YGGCCR 1 cut(s) 1112
AcsI RAATTY 1 cut(s) 368
AcuI CTGAAG 1 cut(s) 1074
AfaI GTAC 2 cut(s) 261, 611
AfiI CCNNNNNNNGG 1 cut(s) 719
AflII CTTAAG 1 cut(s) 668
AgsI TTSAA 5 cut(s) 144, 332, 819, 1202, 1220
AjnI CCWGG 1 cut(s) 188
AluBI AGCT 6 cut(s) 401, 513, 896, 1145, 1207, 1223
AluI AGCT 6 cut(s) 401, 513, 896, 1145, 1207, 1223
Alw26I GTCTC 2 cut(s) 83, 1063
Ama87I CYCGRG 1 cut(s) 719
AoxI GGCC 1 cut(s) 1112
ApeKI GCWGC 3 cut(s) 296, 510, 583
ApoI RAATTY 1 cut(s) 368
Asp700I GAANNNNTTC 1 cut(s) 1206
AsuHPI GGTGA 1 cut(s) 178
AsuII TTCGAA 1 cut(s) 203
AvaI CYCGRG 1 cut(s) 719
BalI TGGCCA 1 cut(s) 1114
BanII GRGCYC 1 cut(s) 737
BbvI GCAGC 3 cut(s) 283, 522, 570
BccI CCATC 2 cut(s) 383, 710
BciT130I CCWGG 1 cut(s) 190
BciVI GTATCC 2 cut(s) 464, 760
BcoDI GTCTC 2 cut(s) 83, 1063
BfaI CTAG 2 cut(s) 35, 1007
BfmI CTRYAG 3 cut(s) 297, 396, 658
BfrI CTTAAG 1 cut(s) 668
BfuAI ACCTGC 2 cut(s) 290, 588
BfuI GTATCC 2 cut(s) 464, 760
BglII AGATCT 1 cut(s) 949
BisI GCNGC 3 cut(s) 297, 511, 584
BlpI GCTNAGC 3 cut(s) 230, 545, 736
BlsI GCNGC 3 cut(s) 298, 512, 585
Bme1390I CCNGG 1 cut(s) 190
BmeT110I CYCGRG 1 cut(s) 719
BmiI GGNNCC 3 cut(s) 493, 700, 711
BmrFI CCNGG 1 cut(s) 190
BmsI GCATC 4 cut(s) 492, 574, 612, 899
Bpu1102I GCTNAGC 3 cut(s) 230, 545, 736
Bpu14I TTCGAA 1 cut(s) 203
BsaI GGTCTC 1 cut(s) 83
BsaXI ACNNNNNCTCC 2 cut(s) 621, 651
Bsc4I CCNNNNNNNGG 1 cut(s) 719
Bse1I ACTGG 2 cut(s) 713, 816
Bse3DI GCAATG 1 cut(s) 185
BseBI CCWGG 1 cut(s) 190
BseLI CCNNNNNNNGG 1 cut(s) 719
BseMI GCAATG 1 cut(s) 185
BseMII CTCAG 3 cut(s) 244, 750, 819
BseNI ACTGG 2 cut(s) 713, 816
BseRI GAGGAG 2 cut(s) 399, 504
BseXI GCAGC 3 cut(s) 283, 522, 570
BseYI CCCAGC 3 cut(s) 284, 348, 843
BsgI GTGCAG 3 cut(s) 270, 905, 1114
Bsh1236I CGCG 1 cut(s) 1171
BshFI GGCC 1 cut(s) 1114
BsiHKCI CYCGRG 1 cut(s) 719
BslFI GGGAC 2 cut(s) 342, 389
BslI CCNNNNNNNGG 1 cut(s) 719
BsmAI GTCTC 2 cut(s) 83, 1063
BsmFI GGGAC 2 cut(s) 342, 389
BsmI GAATGC 1 cut(s) 1070
BsnI GGCC 1 cut(s) 1114
Bso31I GGTCTC 1 cut(s) 83
BsoBI CYCGRG 1 cut(s) 719
Bsp119I TTCGAA 1 cut(s) 203
Bsp1286I GDGCHC 1 cut(s) 737
Bsp143I GATC 2 cut(s) 42, 949
Bsp1720I GCTNAGC 3 cut(s) 230, 545, 736
BspACI CCGC 1 cut(s) 627
BspANI GGCC 1 cut(s) 1114
BspCNI CTCAG 3 cut(s) 243, 749, 818
BspFNI CGCG 1 cut(s) 1171
BspLI GGNNCC 3 cut(s) 493, 700, 711
BspMAI CTGCAG 1 cut(s) 301
BspMI ACCTGC 2 cut(s) 290, 588
BspQI GCTCTTC 2 cut(s) 14, 1197
BspT104I TTCGAA 1 cut(s) 203
BspTI CTTAAG 1 cut(s) 668
BspTNI GGTCTC 1 cut(s) 83
BsrDI GCAATG 1 cut(s) 185
BsrI ACTGG 2 cut(s) 713, 816
BssMI GATC 2 cut(s) 42, 949
Bst2UI CCWGG 1 cut(s) 190
Bst4CI ACNGT 2 cut(s) 70, 77
Bst6I CTCTTC 2 cut(s) 14, 1197
BstAFI CTTAAG 1 cut(s) 668
BstBI TTCGAA 1 cut(s) 203
BstC8I GCNNGC 4 cut(s) 346, 625, 888, 1169
BstDEI CTNAG 5 cut(s) 230, 545, 736, 805, 946
BstEII GGTNACC 1 cut(s) 184
BstFNI CGCG 1 cut(s) 1171
BstKTI GATC 2 cut(s) 45, 952
BstMAI GTCTC 2 cut(s) 83, 1063
BstMBI GATC 2 cut(s) 42, 949
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstNI CCWGG 1 cut(s) 190
BstNSI RCATGY 4 cut(s) 616, 627, 860, 1125
BstPI GGTNACC 1 cut(s) 184
BstSCI CCNGG 1 cut(s) 188
BstSFI CTRYAG 3 cut(s) 297, 396, 658
BstUI CGCG 1 cut(s) 1171
BstV1I GCAGC 3 cut(s) 283, 522, 570
BstX2I RGATCY 1 cut(s) 949
BstYI RGATCY 1 cut(s) 949
BsuI GTATCC 2 cut(s) 464, 760
BsuRI GGCC 1 cut(s) 1114
BtsI GCAGTG 2 cut(s) 131, 495
BtsIMutI CAGTG 3 cut(s) 131, 495, 1018
BveI ACCTGC 2 cut(s) 290, 588
Cac8I GCNNGC 4 cut(s) 346, 625, 888, 1169
CsiI ACCWGGT 1 cut(s) 188
Csp6I GTAC 2 cut(s) 260, 610
CviQI GTAC 2 cut(s) 260, 610
DdeI CTNAG 5 cut(s) 230, 545, 736, 805, 946
DpnI GATC 2 cut(s) 44, 951
DpnII GATC 2 cut(s) 42, 949
EaeI YGGCCR 1 cut(s) 1112
Eam1104I CTCTTC 2 cut(s) 14, 1197
EarI CTCTTC 2 cut(s) 14, 1197
Eco24I GRGCYC 1 cut(s) 737
Eco31I GGTCTC 1 cut(s) 83
Eco57I CTGAAG 1 cut(s) 1074
Eco88I CYCGRG 1 cut(s) 719
Eco91I GGTNACC 1 cut(s) 184
EcoO65I GGTNACC 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 188
EcoT22I ATGCAT 2 cut(s) 567, 1072
EcoT38I GRGCYC 1 cut(s) 737
FalI AAGNNNNNCTT 2 cut(s) 637, 669
FaqI GGGAC 2 cut(s) 342, 389
Fnu4HI GCNGC 3 cut(s) 297, 511, 584
FriOI GRGCYC 1 cut(s) 737
Fsp4HI GCNGC 3 cut(s) 297, 511, 584
FspBI CTAG 2 cut(s) 35, 1007
GluI GCNGC 3 cut(s) 297, 511, 584
GsaI CCCAGC 3 cut(s) 288, 352, 847
HaeIII GGCC 1 cut(s) 1114
HindIII AAGCTT 1 cut(s) 1221
HinfI GANTC 4 cut(s) 838, 864, 1024, 1178
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 3 cut(s) 73, 260, 640
Hpy188I TCNGA 4 cut(s) 66, 488, 989, 1212
Hpy188III TCNNGA 7 cut(s) 40, 52, 379, 574, 807, 916, 1075
Hpy8I GTNNAC 3 cut(s) 73, 260, 640
HpyAV CCTTC 2 cut(s) 58, 210
HpyCH4III ACNGT 2 cut(s) 70, 77
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
HpyF3I CTNAG 5 cut(s) 230, 545, 736, 805, 946
Kzo9I GATC 2 cut(s) 42, 949
LguI GCTCTTC 2 cut(s) 14, 1197
LmnI GCTCC 3 cut(s) 133, 491, 704
Lsp1109I GCAGC 3 cut(s) 283, 522, 570
LweI GCATC 4 cut(s) 492, 574, 612, 899
MabI ACCWGGT 1 cut(s) 188
MaeI CTAG 2 cut(s) 35, 1007
MaeIII GTNAC 3 cut(s) 184, 589, 1048
MalI GATC 2 cut(s) 44, 951
MboI GATC 2 cut(s) 42, 949
MboII GAAGA 3 cut(s) 16, 644, 1214
MflI RGATCY 1 cut(s) 949
MhlI GDGCHC 1 cut(s) 737
MlsI TGGCCA 1 cut(s) 1114
MluCI AATT 6 cut(s) 159, 368, 441, 594, 1032, 1185
MluNI TGGCCA 1 cut(s) 1114
MlyI GAGTC 3 cut(s) 873, 1018, 1172
MmeI TCCRAC 2 cut(s) 841, 967
MnlI CCTC 8 cut(s) 417, 420, 467, 473, 482, 643, 737, 983
Mox20I TGGCCA 1 cut(s) 1114
Mph1103I ATGCAT 2 cut(s) 567, 1072
MroXI GAANNNNTTC 1 cut(s) 1206
MscI TGGCCA 1 cut(s) 1114
MseI TTAA 5 cut(s) 170, 194, 213, 669, 1225
MslI CAYNNNNRTG 1 cut(s) 1120
Msp20I TGGCCA 1 cut(s) 1114
MspA1I CMGCKG 2 cut(s) 401, 896
MspCI CTTAAG 1 cut(s) 668
MspR9I CCNGG 1 cut(s) 190
Mva1269I GAATGC 1 cut(s) 1070
MvaI CCWGG 1 cut(s) 190
MvnI CGCG 1 cut(s) 1171
MwoI GCNNNNNNNGC 1 cut(s) 164
NdeII GATC 2 cut(s) 42, 949
NlaIV GGNNCC 3 cut(s) 493, 700, 711
NmuCI GTSAC 2 cut(s) 184, 589
NsiI ATGCAT 2 cut(s) 567, 1072
NspI RCATGY 4 cut(s) 616, 627, 860, 1125
NspV TTCGAA 1 cut(s) 203
PaeI GCATGC 1 cut(s) 627
PaqCI CACCTGC 1 cut(s) 290
PciSI GCTCTTC 2 cut(s) 14, 1197
PctI GAATGC 1 cut(s) 1070
PdmI GAANNNNTTC 1 cut(s) 1206
PfeI GAWTC 1 cut(s) 838
PkrI GCNGC 3 cut(s) 298, 512, 585
PleI GAGTC 3 cut(s) 872, 1018, 1172
PpsI GAGTC 3 cut(s) 872, 1018, 1172
Psp6I CCWGG 1 cut(s) 188
PspEI GGTNACC 1 cut(s) 184
PspFI CCCAGC 3 cut(s) 284, 348, 843
PspGI CCWGG 1 cut(s) 188
PspN4I GGNNCC 3 cut(s) 493, 700, 711
PstI CTGCAG 1 cut(s) 301
PsuI RGATCY 1 cut(s) 949
PvuII CAGCTG 2 cut(s) 401, 896
RsaI GTAC 2 cut(s) 261, 611
RsaNI GTAC 2 cut(s) 260, 610
RseI CAYNNNNRTG 1 cut(s) 1120
SapI GCTCTTC 2 cut(s) 14, 1197
SaqAI TTAA 5 cut(s) 170, 194, 213, 669, 1225
SatI GCNGC 3 cut(s) 297, 511, 584
Sau3AI GATC 2 cut(s) 42, 949
SchI GAGTC 3 cut(s) 873, 1018, 1172
ScrFI CCNGG 1 cut(s) 190
SduI GDGCHC 1 cut(s) 737
SexAI ACCWGGT 1 cut(s) 188
SfaNI GCATC 4 cut(s) 492, 574, 612, 899
SfcI CTRYAG 3 cut(s) 297, 396, 658
SfuI TTCGAA 1 cut(s) 203
SmiMI CAYNNNNRTG 1 cut(s) 1120
SmlI CTYRAG 1 cut(s) 668
SmoI CTYRAG 1 cut(s) 668
SphI GCATGC 1 cut(s) 627
Sse9I AATT 6 cut(s) 159, 368, 441, 594, 1032, 1185
SsiI CCGC 1 cut(s) 627
SspMI CTAG 2 cut(s) 35, 1007
StyD4I CCNGG 1 cut(s) 188
TaaI ACNGT 2 cut(s) 70, 77
TaqI TCGA 4 cut(s) 86, 203, 366, 942
TasI AATT 6 cut(s) 159, 368, 441, 594, 1032, 1185
TatI WGTACW 2 cut(s) 259, 609
TfiI GAWTC 1 cut(s) 838
Tru1I TTAA 5 cut(s) 170, 194, 213, 669, 1225
Tru9I TTAA 5 cut(s) 170, 194, 213, 669, 1225
TscAI CASTG 3 cut(s) 138, 502, 1018
TseFI GTSAC 2 cut(s) 184, 589
TseI GCWGC 3 cut(s) 296, 510, 583
Tsp45I GTSAC 2 cut(s) 184, 589
TspDTI ATGAA 5 cut(s) 316, 764, 777, 1025, 1078
TspRI CASTG 3 cut(s) 138, 502, 1018
Vha464I CTTAAG 1 cut(s) 668
XapI RAATTY 1 cut(s) 368
XceI RCATGY 4 cut(s) 616, 627, 860, 1125
XmnI GAANNNNTTC 1 cut(s) 1206
XspI CTAG 2 cut(s) 35, 1007
Zsp2I ATGCAT 2 cut(s) 567, 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.