Rh3DG361600

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0004978
Physical Location & Seq
Forward (+)
44799900 .. 44801760
1861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG361600.1

Sequence Viewer

Length: 1464 bp
ATGGCTGACTTTTCGTCCATCTCTGCCCCCAATCCCCCATTAGAGTACGTGAGGAACAAGGCAATTCTATATAAAGACTCGGTCAGTTCATTCAACAATTTAAAAAGAGTGGAACAGGTTTTTATTAAGTCCAAGTGGATAAAACATATAGTTATTTCAAATTTCCATCAGATTTTGTTAAATCTTGGAAGCAAAGAAAGTGGCGTGAGAAGAAATCCGCAGAGACTTATTTTAAGACGATTGATGGCAACTACAGAGAACATCCTCAAACAAGTTCAAGAAGAAGTAGGCCACGAACCACATCAAGTTTACGGTCTCATTTCCACCAATCACAAGCACCATTTCCACCCACTACAGTTCTATGCTCCACTGCTCACTTCAACACTCTGCTCTGCAATTGTCGCTTTAATATGGCAATGGGTCACCAGGTTAAGCCCTTCGAAAGCCATTAAAACAGCCTTTTGGCTCAGCCCCTTGCTAACCTGCGCAGTTGGTGTACTTCTCATATCTATAAGTTCTGCTGGGAGTTTGGTTGCAGGTGGGGTTGCAATCATCTGTGCGCTCATTCAATCGCTGTATGCCTGCTGGGTCAGTCCCCGTATCGAATTTGCCATCAGGATATTATCTGTTTCTACAGCTGTCCCTCCTCCCAAAATCACAACATTACTCATTCTATCAATTTTTACCTGTATCCTCTACCTCTCTTTCTTGGTATCCGGCATCGGAGGAGCCACTGCCATAAGAAGCAGCTTTAGTGTCCTGTTTATCTCGGTTATCTTGCTGAGCATGGCATGGACTATGCATCTCATCAAGAACCTGCTGCAAGTCACAATTTCACGCATCAAGTACATGCACTTTGCATGCGGAGAAGATGTTGACACAAGTGAGGCTTTCTGCAACACACTTAAGCACTCAATGGGGAGTATCTGCATTGGCTCCATCTTGGTTCCAGTTCTCGGAGTTATTCGGGGATCAGCCAGAGGCATGAAATTGGTTGCAGGGGATACAGACGAGTTTCTATTTTCATGCGCCAACTGCTATTCGGGACTGGCTTCGACTCTGGTGATGTATGGGAATCGCTGGGGTTTTGTGCATGTTGGAGTCTATAACAAGGGCTTTGTGCAGGCATCAGCTGATACATGGGAGATGTTCAGGAATATAGAACTAAGAGAAATAATCGACTTAGATCTCACTACATCGTTCTGTTTTCTTTCTGGGGTGGTCGGAGGTGCAATATGTAGCCTAGTCAGTGGAACTTGGACTCTTGCAATTCATAAGAGTTATGCTACTGAAGTCTCCTTATATGCATTCTTGATCGGCTATTTCATGTGCAGGATTGCTGTGGCATGGCCACAAGCATGTGTTTTGTCTTACTACGTAGCTTATGCAGAGAACCCTAATAGCACGCGATTTGACTCAACACTTCCTGTTCGCATTGAAGAGCTTCGGAGATTTCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

487

Amino Acids

53.93

Weight (kDa)

9.06

Isoelectric Point (pI)

48.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 182 - 466 3.6e-27 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 527
Acc16I TGCGCA 1 cut(s) 487
Acc36I ACCTGC 3 cut(s) 491, 527, 825
AccII CGCG 1 cut(s) 1408
AciI CCGC 2 cut(s) 218, 864
AclWI GGATC 1 cut(s) 979
AcoI YGGCCR 1 cut(s) 1349
AcsI RAATTY 2 cut(s) 160, 605
AcuI CTGAAG 1 cut(s) 1311
AfaI GTAC 3 cut(s) 47, 498, 848
AfiI CCNNNNNNNGG 1 cut(s) 956
AflII CTTAAG 1 cut(s) 905
AgsI TTSAA 7 cut(s) 94, 159, 278, 381, 569, 1439, 1457
AhdI GACNNNNNGTC 1 cut(s) 13
AjnI CCWGG 1 cut(s) 425
AluBI AGCT 6 cut(s) 638, 750, 1133, 1382, 1444, 1460
AluI AGCT 6 cut(s) 638, 750, 1133, 1382, 1444, 1460
Alw26I GTCTC 3 cut(s) 217, 320, 1300
AlwI GGATC 1 cut(s) 979
AoxI GGCC 2 cut(s) 289, 1349
ApeKI GCWGC 2 cut(s) 747, 820
ApoI RAATTY 2 cut(s) 160, 605
Asp700I GAANNNNTTC 1 cut(s) 1443
AspLEI GCGC 3 cut(s) 488, 562, 1031
AsuHPI GGTGA 2 cut(s) 415, 1075
AsuII TTCGAA 1 cut(s) 440
BalI TGGCCA 1 cut(s) 1351
BbvI GCAGC 2 cut(s) 759, 807
BccI CCATC 5 cut(s) 26, 174, 238, 620, 947
BciT130I CCWGG 1 cut(s) 427
BciVI GTATCC 3 cut(s) 701, 724, 997
BcoDI GTCTC 3 cut(s) 217, 320, 1300
BfaI CTAG 1 cut(s) 1244
BfmI CTRYAG 3 cut(s) 252, 353, 633
BfrI CTTAAG 1 cut(s) 905
BfuAI ACCTGC 3 cut(s) 491, 527, 825
BfuI GTATCC 3 cut(s) 701, 724, 997
BglII AGATCT 1 cut(s) 1186
BisI GCNGC 2 cut(s) 748, 821
BlpI GCTNAGC 2 cut(s) 467, 782
BlsI GCNGC 2 cut(s) 749, 822
Bme1390I CCNGG 1 cut(s) 427
BmeRI GACNNNNNGTC 1 cut(s) 13
BmiI GGNNCC 3 cut(s) 730, 937, 948
BmrFI CCNGG 1 cut(s) 427
BmsI GCATC 4 cut(s) 729, 811, 849, 1136
Bpu1102I GCTNAGC 2 cut(s) 467, 782
Bpu14I TTCGAA 1 cut(s) 440
BsaAI YACGTR 2 cut(s) 49, 1378
BsaI GGTCTC 1 cut(s) 320
BsaXI ACNNNNNCTCC 2 cut(s) 858, 888
Bsc4I CCNNNNNNNGG 1 cut(s) 956
Bse1I ACTGG 2 cut(s) 950, 1053
Bse3DI GCAATG 1 cut(s) 422
BseBI CCWGG 1 cut(s) 427
BseGI GGATG 1 cut(s) 261
BseLI CCNNNNNNNGG 1 cut(s) 956
BseMI GCAATG 1 cut(s) 422
BseMII CTCAG 2 cut(s) 481, 773
BseNI ACTGG 2 cut(s) 950, 1053
BseRI GAGGAG 2 cut(s) 636, 741
BseXI GCAGC 2 cut(s) 759, 807
BseYI CCCAGC 3 cut(s) 521, 585, 1080
BsgI GTGCAG 2 cut(s) 1142, 1351
Bsh1236I CGCG 1 cut(s) 1408
BshFI GGCC 2 cut(s) 291, 1351
BsiSI CCGG 1 cut(s) 717
BslFI GGGAC 3 cut(s) 579, 626, 1059
BslI CCNNNNNNNGG 1 cut(s) 956
BsmAI GTCTC 3 cut(s) 217, 320, 1300
BsmFI GGGAC 3 cut(s) 579, 626, 1059
BsmI GAATGC 1 cut(s) 1307
BsnI GGCC 2 cut(s) 291, 1351
Bso31I GGTCTC 1 cut(s) 320
Bsp119I TTCGAA 1 cut(s) 440
Bsp143I GATC 3 cut(s) 971, 1186, 1314
Bsp1720I GCTNAGC 2 cut(s) 467, 782
BspACI CCGC 2 cut(s) 218, 864
BspANI GGCC 2 cut(s) 291, 1351
BspCNI CTCAG 2 cut(s) 480, 774
BspFNI CGCG 1 cut(s) 1408
BspLI GGNNCC 3 cut(s) 730, 937, 948
BspMI ACCTGC 3 cut(s) 491, 527, 825
BspPI GGATC 1 cut(s) 979
BspQI GCTCTTC 1 cut(s) 1434
BspT104I TTCGAA 1 cut(s) 440
BspTI CTTAAG 1 cut(s) 905
BspTNI GGTCTC 1 cut(s) 320
BsrDI GCAATG 1 cut(s) 422
BsrI ACTGG 2 cut(s) 950, 1053
BssMI GATC 3 cut(s) 971, 1186, 1314
Bst2UI CCWGG 1 cut(s) 427
Bst4CI ACNGT 2 cut(s) 314, 357
Bst6I CTCTTC 1 cut(s) 1434
BstAFI CTTAAG 1 cut(s) 905
BstBAI YACGTR 2 cut(s) 49, 1378
BstBI TTCGAA 1 cut(s) 440
BstC8I GCNNGC 4 cut(s) 583, 862, 1125, 1406
BstDEI CTNAG 4 cut(s) 467, 782, 1166, 1183
BstEII GGTNACC 1 cut(s) 421
BstF5I GGATG 1 cut(s) 261
BstFNI CGCG 1 cut(s) 1408
BstHHI GCGC 3 cut(s) 488, 562, 1031
BstKTI GATC 3 cut(s) 974, 1189, 1317
BstMAI GTCTC 3 cut(s) 217, 320, 1300
BstMBI GATC 3 cut(s) 971, 1186, 1314
BstMWI GCNNNNNNNGC 2 cut(s) 401, 1035
BstNI CCWGG 1 cut(s) 427
BstNSI RCATGY 4 cut(s) 853, 864, 1097, 1362
BstPI GGTNACC 1 cut(s) 421
BstSCI CCNGG 1 cut(s) 425
BstSFI CTRYAG 3 cut(s) 252, 353, 633
BstSNI TACGTA 1 cut(s) 1378
BstUI CGCG 1 cut(s) 1408
BstV1I GCAGC 2 cut(s) 759, 807
BstX2I RGATCY 1 cut(s) 1186
BstYI RGATCY 1 cut(s) 1186
BsuI GTATCC 3 cut(s) 701, 724, 997
BsuRI GGCC 2 cut(s) 291, 1351
BtsCI GGATG 1 cut(s) 261
BtsI GCAGTG 2 cut(s) 368, 732
BtsIMutI CAGTG 3 cut(s) 368, 732, 1255
BveI ACCTGC 3 cut(s) 491, 527, 825
Cac8I GCNNGC 4 cut(s) 583, 862, 1125, 1406
CfoI GCGC 3 cut(s) 488, 562, 1031
CsiI ACCWGGT 1 cut(s) 425
Csp6I GTAC 3 cut(s) 46, 497, 847
CspCI CAANNNNNGTGG 2 cut(s) 181, 216
CviQI GTAC 3 cut(s) 46, 497, 847
DdeI CTNAG 4 cut(s) 467, 782, 1166, 1183
DpnI GATC 3 cut(s) 973, 1188, 1316
DpnII GATC 3 cut(s) 971, 1186, 1314
DraI TTTAAA 1 cut(s) 102
DriI GACNNNNNGTC 1 cut(s) 13
EaeI YGGCCR 1 cut(s) 1349
Eam1104I CTCTTC 1 cut(s) 1434
Eam1105I GACNNNNNGTC 1 cut(s) 13
EarI CTCTTC 1 cut(s) 1434
Eco105I TACGTA 1 cut(s) 1378
Eco31I GGTCTC 1 cut(s) 320
Eco57I CTGAAG 1 cut(s) 1311
Eco91I GGTNACC 1 cut(s) 421
EcoO65I GGTNACC 1 cut(s) 421
EcoRII CCWGG 1 cut(s) 425
EcoT22I ATGCAT 2 cut(s) 804, 1309
FalI AAGNNNNNCTT 2 cut(s) 874, 906
FaqI GGGAC 3 cut(s) 579, 626, 1059
Fnu4HI GCNGC 2 cut(s) 748, 821
FokI GGATG 1 cut(s) 248
Fsp4HI GCNGC 2 cut(s) 748, 821
FspBI CTAG 1 cut(s) 1244
FspI TGCGCA 1 cut(s) 487
GlaI GCGC 3 cut(s) 487, 561, 1030
GluI GCNGC 2 cut(s) 748, 821
GsaI CCCAGC 3 cut(s) 525, 589, 1084
HaeIII GGCC 2 cut(s) 291, 1351
HapII CCGG 1 cut(s) 717
HhaI GCGC 3 cut(s) 488, 562, 1031
Hin6I GCGC 3 cut(s) 486, 560, 1029
HinP1I GCGC 3 cut(s) 486, 560, 1029
HincII GTYRAC 1 cut(s) 877
HindII GTYRAC 1 cut(s) 877
HindIII AAGCTT 1 cut(s) 1458
HinfI GANTC 6 cut(s) 77, 1057, 1075, 1101, 1261, 1415
HpaII CCGG 1 cut(s) 717
HphI GGTGA 2 cut(s) 415, 1075
Hpy166II GTNNAC 3 cut(s) 310, 497, 877
Hpy188I TCNGA 5 cut(s) 171, 725, 959, 1226, 1449
Hpy188III TCNNGA 6 cut(s) 278, 616, 811, 1044, 1153, 1312
Hpy8I GTNNAC 3 cut(s) 310, 497, 877
HpyAV CCTTC 1 cut(s) 447
HpyCH4III ACNGT 2 cut(s) 314, 357
HpyCH4IV ACGT 2 cut(s) 48, 1377
HpyF10VI GCNNNNNNNGC 2 cut(s) 401, 1035
HpyF3I CTNAG 4 cut(s) 467, 782, 1166, 1183
HpySE526I ACGT 2 cut(s) 48, 1377
HspAI GCGC 3 cut(s) 486, 560, 1029
Kzo9I GATC 3 cut(s) 971, 1186, 1314
LguI GCTCTTC 1 cut(s) 1434
LmnI GCTCC 3 cut(s) 370, 728, 941
Lsp1109I GCAGC 2 cut(s) 759, 807
LweI GCATC 4 cut(s) 729, 811, 849, 1136
MabI ACCWGGT 1 cut(s) 425
MaeI CTAG 1 cut(s) 1244
MaeII ACGT 2 cut(s) 48, 1377
MaeIII GTNAC 2 cut(s) 421, 826
MalI GATC 3 cut(s) 973, 1188, 1316
MboI GATC 3 cut(s) 971, 1186, 1314
MboII GAAGA 4 cut(s) 222, 293, 881, 1451
MfeI CAATTG 1 cut(s) 396
MflI RGATCY 1 cut(s) 1186
MlsI TGGCCA 1 cut(s) 1351
MluCI AATT 9 cut(s) 63, 97, 160, 396, 605, 678, 831, 989, 1269
MluNI TGGCCA 1 cut(s) 1351
MlyI GAGTC 5 cut(s) 71, 1051, 1110, 1255, 1409
MmeI TCCRAC 2 cut(s) 1078, 1204
Mox20I TGGCCA 1 cut(s) 1351
Mph1103I ATGCAT 2 cut(s) 804, 1309
MroXI GAANNNNTTC 1 cut(s) 1443
MscI TGGCCA 1 cut(s) 1351
MseI TTAA 9 cut(s) 101, 126, 179, 233, 407, 431, 450, 906, 1462
MslI CAYNNNNRTG 1 cut(s) 1357
Msp20I TGGCCA 1 cut(s) 1351
MspA1I CMGCKG 2 cut(s) 638, 1133
MspCI CTTAAG 1 cut(s) 905
MspI CCGG 1 cut(s) 717
MspR9I CCNGG 1 cut(s) 427
MunI CAATTG 1 cut(s) 396
Mva1269I GAATGC 1 cut(s) 1307
MvaI CCWGG 1 cut(s) 427
MvnI CGCG 1 cut(s) 1408
MwoI GCNNNNNNNGC 2 cut(s) 401, 1035
NdeII GATC 3 cut(s) 971, 1186, 1314
NlaIV GGNNCC 3 cut(s) 730, 937, 948
NmuCI GTSAC 2 cut(s) 421, 826
NsbI TGCGCA 1 cut(s) 487
NsiI ATGCAT 2 cut(s) 804, 1309
NspI RCATGY 4 cut(s) 853, 864, 1097, 1362
NspV TTCGAA 1 cut(s) 440
PaeI GCATGC 1 cut(s) 864
PaqCI CACCTGC 1 cut(s) 527
PciSI GCTCTTC 1 cut(s) 1434
PctI GAATGC 1 cut(s) 1307
PdmI GAANNNNTTC 1 cut(s) 1443
PfeI GAWTC 1 cut(s) 1075
PflFI GACNNNGTC 1 cut(s) 80
PkrI GCNGC 2 cut(s) 749, 822
PleI GAGTC 5 cut(s) 71, 1051, 1109, 1255, 1409
PpsI GAGTC 5 cut(s) 71, 1051, 1109, 1255, 1409
Ppu21I YACGTR 2 cut(s) 49, 1378
Psp6I CCWGG 1 cut(s) 425
PspEI GGTNACC 1 cut(s) 421
PspFI CCCAGC 3 cut(s) 521, 585, 1080
PspGI CCWGG 1 cut(s) 425
PspN4I GGNNCC 3 cut(s) 730, 937, 948
PsuI RGATCY 1 cut(s) 1186
PsyI GACNNNGTC 1 cut(s) 80
PvuII CAGCTG 2 cut(s) 638, 1133
RsaI GTAC 3 cut(s) 47, 498, 848
RsaNI GTAC 3 cut(s) 46, 497, 847
RseI CAYNNNNRTG 1 cut(s) 1357
SapI GCTCTTC 1 cut(s) 1434
SaqAI TTAA 9 cut(s) 101, 126, 179, 233, 407, 431, 450, 906, 1462
SatI GCNGC 2 cut(s) 748, 821
Sau3AI GATC 3 cut(s) 971, 1186, 1314
SchI GAGTC 5 cut(s) 71, 1051, 1110, 1255, 1409
ScrFI CCNGG 1 cut(s) 427
SexAI ACCWGGT 1 cut(s) 425
SfaNI GCATC 4 cut(s) 729, 811, 849, 1136
SfcI CTRYAG 3 cut(s) 252, 353, 633
SfuI TTCGAA 1 cut(s) 440
SmiMI CAYNNNNRTG 1 cut(s) 1357
SmlI CTYRAG 1 cut(s) 905
SmoI CTYRAG 1 cut(s) 905
SnaBI TACGTA 1 cut(s) 1378
SphI GCATGC 1 cut(s) 864
Sse9I AATT 9 cut(s) 63, 97, 160, 396, 605, 678, 831, 989, 1269
SsiI CCGC 2 cut(s) 218, 864
SspMI CTAG 1 cut(s) 1244
StyD4I CCNGG 1 cut(s) 425
TaaI ACNGT 2 cut(s) 314, 357
TaiI ACGT 2 cut(s) 51, 1380
TaqI TCGA 4 cut(s) 440, 603, 1055, 1179
TaqII GACCGA 1 cut(s) 70
TasI AATT 9 cut(s) 63, 97, 160, 396, 605, 678, 831, 989, 1269
TatI WGTACW 2 cut(s) 496, 846
TfiI GAWTC 1 cut(s) 1075
Tru1I TTAA 9 cut(s) 101, 126, 179, 233, 407, 431, 450, 906, 1462
Tru9I TTAA 9 cut(s) 101, 126, 179, 233, 407, 431, 450, 906, 1462
TscAI CASTG 3 cut(s) 375, 739, 1255
TseFI GTSAC 2 cut(s) 421, 826
TseI GCWGC 2 cut(s) 747, 820
Tsp45I GTSAC 2 cut(s) 421, 826
TspDTI ATGAA 5 cut(s) 78, 1001, 1014, 1262, 1315
TspRI CASTG 3 cut(s) 375, 739, 1255
Tth111I GACNNNGTC 1 cut(s) 80
Vha464I CTTAAG 1 cut(s) 905
XapI RAATTY 2 cut(s) 160, 605
XceI RCATGY 4 cut(s) 853, 864, 1097, 1362
XmnI GAANNNNTTC 1 cut(s) 1443
XspI CTAG 1 cut(s) 1244
Zsp2I ATGCAT 2 cut(s) 804, 1309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.