pycom14g00810

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004978
Physical Location & Seq
Reverse (-)
547270 .. 548551
1282 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g00810.1

Sequence Viewer

Length: 1122 bp
ATGGCAAGTTTAGAGACCACCACGAACATCCACATGCAAGTTCAAGTCAGACCTTCATCCGTAAACCAAATCAAGACGCAAGAACCTATCGACATTCCAACCGAAACAACCACGGCAGGACAATTCCTCAGGAGGTTCTTTAAACTTCTTTTTTACTTGCACTTGATTTTCATCACCATCTTCGTAATCTTCCTTACGCTCTATGGCCTTATCTTCACCACTCGCACTCGCCATTTCCGACCCCTCGAGTATTACCCTCCATTGCTCACTGCAATTGGGTGTTCTGCAACTGTTGCTTTCATATGGCAGTGGATCACTAGCTCAAGCCCTTCAAAAGCCATCCAGGTAGCCTTCTCGCTCAGCCCTCTGCTAACATGCGCAGTTGGTGTACTACTTATATACATAAGTTCTCGTGTAAGTTTGGCAGCGGGTGTGATTGCCATCGTTTGTGCCCTCATTCAGTCTCTGTATGGCTGTTGGGTCAGTCCCCGTTTTAACTACGCCATTCGAGTTTTATCAGTTTCCACTGCTTTTCCTCCTCCGAAAACTACAATACTAGTCATTCAATCAATTTTCGTCAGCATTCTTTACTTGTGTCTCTTGGTTGCCGGCATTGGAAGAGCCACTGCCATCAGAAGTAGTTGGAAGGCCCTGTTCATCGCAGTAATATTGCTAAGCATGGCATGGACGATGCAGGTAATCAAGAACACGCTGCTAGTTACCATCTCACGAATCAAGTATATGAACTTCGCATTTGGAATAGAGATTGACACACGTGAGGCTTCCCGCGACACCATTAAGCACTTTACTGGGAGTATTTGCATCGGCTCCATCTTGGTTCCGGTTCTTGGGGTTGTTCGTGGCTCAGCCCGAGCCATGAAACTGATTGCAGGGGACACCGACGAGTTTCTGTTTTCGTGTGCCAACTGCTATTCAGGAATGGCTTCGACGCTGGTGATGTACGGGAATCGCTGGGGTTTTGTGCACGTAGGAGCGTATAATAAGGGGTTTGTGCAGGCATCATCAGATACTTGGGAGAAGTTCGAGAACGAAGGACTGAAAGAATTAATCGACTCGGATCGATCACCGGATCGTTCTGTTTTCTTTCTGGGGTGGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

374

Amino Acids

41.52

Weight (kDa)

9.35

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 132 - 360 1.7e-15 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 379
Acc36I ACCTGC 1 cut(s) 685
AccII CGCG 1 cut(s) 789
AciI CCGC 2 cut(s) 428, 787
AclWI GGATC 3 cut(s) 320, 1086, 1098
AcvI CACGTG 1 cut(s) 776
AfaI GTAC 2 cut(s) 390, 962
AfiI CCNNNNNNNGG 1 cut(s) 848
AflIII ACRYGT 1 cut(s) 773
AgsI TTSAA 3 cut(s) 44, 333, 566
AhlI ACTAGT 1 cut(s) 556
AjnI CCWGG 1 cut(s) 342
AluBI AGCT 1 cut(s) 321
AluI AGCT 1 cut(s) 321
Alw21I GWGCWC 1 cut(s) 987
Alw26I GTCTC 3 cut(s) 8, 468, 602
Alw44I GTGCAC 1 cut(s) 983
AlwI GGATC 3 cut(s) 320, 1086, 1098
AlwNI CAGNNNCTG 1 cut(s) 466
Ama87I CYCGRG 2 cut(s) 245, 870
AoxI GGCC 2 cut(s) 205, 648
ApaLI GTGCAC 1 cut(s) 983
ApeKI GCWGC 2 cut(s) 425, 712
AseI ATTAAT 1 cut(s) 1067
Asp700I GAANNNNTTC 1 cut(s) 943
AspLEI GCGC 1 cut(s) 380
AspS9I GGNCC 1 cut(s) 649
AsuHPI GGTGA 4 cut(s) 166, 208, 967, 1077
AvaI CYCGRG 2 cut(s) 245, 870
AxyI CCTNAGG 1 cut(s) 128
BaeGI GKGCMC 2 cut(s) 454, 987
BarI GAAGNNNNNNTAC 2 cut(s) 731, 763
BauI CACGAG 1 cut(s) 411
BbrPI CACGTG 1 cut(s) 776
Bbv12I GWGCWC 1 cut(s) 987
BbvI GCAGC 2 cut(s) 437, 699
BccI CCATC 6 cut(s) 185, 347, 449, 638, 731, 839
BceAI ACGGC 1 cut(s) 129
BciT130I CCWGG 1 cut(s) 344
BcoDI GTCTC 3 cut(s) 8, 468, 602
BcuI ACTAGT 1 cut(s) 556
BfaI CTAG 3 cut(s) 318, 557, 716
BfuAI ACCTGC 1 cut(s) 685
BisI GCNGC 2 cut(s) 426, 713
BlpI GCTNAGC 3 cut(s) 359, 674, 865
BlsI GCNGC 2 cut(s) 427, 714
Bme1390I CCNGG 1 cut(s) 344
BmeT110I CYCGRG 2 cut(s) 245, 870
BmgT120I GGNCC 1 cut(s) 649
BmiI GGNNCC 2 cut(s) 829, 840
BmrFI CCNGG 1 cut(s) 344
BmrI ACTGGG 1 cut(s) 819
BmsI GCATC 3 cut(s) 681, 831, 1028
BmuI ACTGGG 1 cut(s) 819
Bpu1102I GCTNAGC 3 cut(s) 359, 674, 865
BpuEI CTTGAG 1 cut(s) 307
Bsa29I ATCGAT 1 cut(s) 1081
BsaAI YACGTR 2 cut(s) 776, 988
BsaBI GATNNNNATC 2 cut(s) 170, 440
BsaI GGTCTC 1 cut(s) 8
BsaJI CCNNGG 1 cut(s) 111
BsaWI WCCGGW 2 cut(s) 841, 1087
Bsc4I CCNNNNNNNGG 1 cut(s) 848
Bse118I RCCGGY 1 cut(s) 608
Bse1I ACTGG 1 cut(s) 814
Bse21I CCTNAGG 1 cut(s) 128
Bse3DI GCAATG 1 cut(s) 260
Bse8I GATNNNNATC 2 cut(s) 170, 440
BseBI CCWGG 1 cut(s) 344
BseCI ATCGAT 1 cut(s) 1081
BseDI CCNNGG 1 cut(s) 111
BseGI GGATG 3 cut(s) 27, 56, 339
BseJI GATNNNNATC 2 cut(s) 170, 440
BseLI CCNNNNNNNGG 1 cut(s) 848
BseMI GCAATG 1 cut(s) 260
BseMII CTCAG 3 cut(s) 142, 373, 879
BseNI ACTGG 1 cut(s) 814
BseRI GAGGAG 1 cut(s) 528
BseSI GKGCMC 2 cut(s) 454, 987
BseXI GCAGC 2 cut(s) 437, 699
BseYI CCCAGC 1 cut(s) 972
BsgI GTGCAG 1 cut(s) 1034
Bsh1236I CGCG 1 cut(s) 789
BshFI GGCC 2 cut(s) 207, 650
BshVI ATCGAT 1 cut(s) 1081
BsiHKAI GWGCWC 1 cut(s) 987
BsiHKCI CYCGRG 2 cut(s) 245, 870
BsiSI CCGG 3 cut(s) 609, 842, 1088
BslFI GGGAC 2 cut(s) 471, 908
BslI CCNNNNNNNGG 1 cut(s) 848
BsmAI GTCTC 3 cut(s) 8, 468, 602
BsmFI GGGAC 2 cut(s) 471, 908
BsmI GAATGC 1 cut(s) 582
BsnI GGCC 2 cut(s) 207, 650
Bso31I GGTCTC 1 cut(s) 8
BsoBI CYCGRG 2 cut(s) 245, 870
Bsp1286I GDGCHC 2 cut(s) 454, 987
Bsp143I GATC 4 cut(s) 312, 1078, 1082, 1090
Bsp1720I GCTNAGC 3 cut(s) 359, 674, 865
BspACI CCGC 2 cut(s) 428, 787
BspANI GGCC 2 cut(s) 207, 650
BspCNI CTCAG 3 cut(s) 141, 372, 878
BspDI ATCGAT 1 cut(s) 1081
BspFNI CGCG 1 cut(s) 789
BspLI GGNNCC 2 cut(s) 829, 840
BspMI ACCTGC 1 cut(s) 685
BspPI GGATC 3 cut(s) 320, 1086, 1098
BspQI GCTCTTC 1 cut(s) 613
BspTNI GGTCTC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 260
BsrFI RCCGGY 1 cut(s) 608
BsrI ACTGG 1 cut(s) 814
BssAI RCCGGY 1 cut(s) 608
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 4 cut(s) 312, 1078, 1082, 1090
BssSI CACGAG 1 cut(s) 411
Bst2BI CACGAG 1 cut(s) 411
Bst2UI CCWGG 1 cut(s) 344
Bst4CI ACNGT 1 cut(s) 292
Bst6I CTCTTC 1 cut(s) 613
BstAPI GCANNNNNTGC 1 cut(s) 293
BstBAI YACGTR 2 cut(s) 776, 988
BstC8I GCNNGC 2 cut(s) 610, 1017
BstDEI CTNAG 4 cut(s) 128, 359, 674, 865
BstDSI CCRYGG 1 cut(s) 111
BstF5I GGATG 3 cut(s) 27, 56, 339
BstFNI CGCG 1 cut(s) 789
BstHHI GCGC 1 cut(s) 380
BstKTI GATC 4 cut(s) 315, 1081, 1085, 1093
BstMAI GTCTC 3 cut(s) 8, 468, 602
BstMBI GATC 4 cut(s) 312, 1078, 1082, 1090
BstMWI GCNNNNNNNGC 1 cut(s) 293
BstNI CCWGG 1 cut(s) 344
BstNSI RCATGY 2 cut(s) 37, 378
BstSCI CCNGG 1 cut(s) 342
BstSLI GKGCMC 2 cut(s) 454, 987
BstUI CGCG 1 cut(s) 789
BstV1I GCAGC 2 cut(s) 437, 699
Bsu15I ATCGAT 1 cut(s) 1081
Bsu36I CCTNAGG 1 cut(s) 128
BsuRI GGCC 2 cut(s) 207, 650
BsuTUI ATCGAT 1 cut(s) 1081
BtgI CCRYGG 1 cut(s) 111
BtgZI GCGATG 1 cut(s) 643
BtsCI GGATG 3 cut(s) 27, 56, 339
BtsI GCAGTG 4 cut(s) 267, 314, 525, 624
BtsIMutI CAGTG 4 cut(s) 267, 314, 525, 624
BveI ACCTGC 1 cut(s) 685
Cac8I GCNNGC 2 cut(s) 610, 1017
CaiI CAGNNNCTG 1 cut(s) 466
CfoI GCGC 1 cut(s) 380
Cfr10I RCCGGY 1 cut(s) 608
Cfr13I GGNCC 1 cut(s) 649
ClaI ATCGAT 1 cut(s) 1081
CseI GACGC 2 cut(s) 85, 958
Csp6I GTAC 2 cut(s) 389, 961
CviAII CATG 5 cut(s) 34, 375, 679, 684, 877
CviQI GTAC 2 cut(s) 389, 961
DdeI CTNAG 4 cut(s) 128, 359, 674, 865
DpnI GATC 4 cut(s) 314, 1080, 1084, 1092
DpnII GATC 4 cut(s) 312, 1078, 1082, 1090
DraI TTTAAA 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 613
EarI CTCTTC 1 cut(s) 613
Eco31I GGTCTC 1 cut(s) 8
Eco72I CACGTG 1 cut(s) 776
Eco81I CCTNAGG 1 cut(s) 128
Eco88I CYCGRG 2 cut(s) 245, 870
EcoO109I RGGNCCY 1 cut(s) 649
EcoRII CCWGG 1 cut(s) 342
FaeI CATG 5 cut(s) 37, 378, 682, 687, 880
FaqI GGGAC 2 cut(s) 471, 908
FatI CATG 5 cut(s) 33, 374, 678, 683, 876
FauI CCCGC 2 cut(s) 421, 794
FauNDI CATATG 1 cut(s) 302
Fnu4HI GCNGC 2 cut(s) 426, 713
FokI GGATG 3 cut(s) 14, 43, 326
Fsp4HI GCNGC 2 cut(s) 426, 713
FspBI CTAG 3 cut(s) 318, 557, 716
FspI TGCGCA 1 cut(s) 379
GlaI GCGC 1 cut(s) 379
GluI GCNGC 2 cut(s) 426, 713
GsaI CCCAGC 1 cut(s) 976
HaeIII GGCC 2 cut(s) 207, 650
HapII CCGG 3 cut(s) 609, 842, 1088
HgaI GACGC 2 cut(s) 85, 958
HhaI GCGC 1 cut(s) 380
Hin1II CATG 5 cut(s) 37, 378, 682, 687, 880
Hin6I GCGC 1 cut(s) 378
HinP1I GCGC 1 cut(s) 378
HinfI GANTC 3 cut(s) 732, 967, 1073
HpaII CCGG 3 cut(s) 609, 842, 1088
HphI GGTGA 4 cut(s) 166, 208, 967, 1077
Hpy166II GTNNAC 3 cut(s) 64, 389, 985
Hpy188I TCNGA 6 cut(s) 50, 239, 543, 635, 1027, 1078
Hpy188III TCNNGA 6 cut(s) 73, 130, 703, 729, 936, 1045
Hpy8I GTNNAC 3 cut(s) 64, 389, 985
Hpy99I CGWCG 2 cut(s) 905, 952
HpyAV CCTTC 5 cut(s) 63, 339, 361, 640, 1046
HpyCH4III ACNGT 1 cut(s) 292
HpyCH4IV ACGT 2 cut(s) 775, 987
HpyCH4V TGCA 9 cut(s) 37, 160, 272, 287, 694, 822, 890, 985, 1015
HpyF10VI GCNNNNNNNGC 1 cut(s) 293
HpyF3I CTNAG 4 cut(s) 128, 359, 674, 865
HpySE526I ACGT 2 cut(s) 775, 987
Hsp92II CATG 5 cut(s) 37, 378, 682, 687, 880
HspAI GCGC 1 cut(s) 378
KroI GCCGGC 1 cut(s) 608
KroNI GCCGGC 1 cut(s) 610
Kzo9I GATC 4 cut(s) 312, 1078, 1082, 1090
LguI GCTCTTC 1 cut(s) 613
LmnI GCTCC 2 cut(s) 833, 992
Lsp1109I GCAGC 2 cut(s) 437, 699
LweI GCATC 3 cut(s) 681, 831, 1028
MaeI CTAG 3 cut(s) 318, 557, 716
MaeII ACGT 2 cut(s) 775, 987
MaeIII GTNAC 1 cut(s) 718
MalI GATC 4 cut(s) 314, 1080, 1084, 1092
MboI GATC 4 cut(s) 312, 1078, 1082, 1090
MboII GAAGA 4 cut(s) 172, 181, 205, 630
MfeI CAATTG 1 cut(s) 273
MhlI GDGCHC 2 cut(s) 454, 987
MluCI AATT 4 cut(s) 122, 273, 570, 1064
MlyI GAGTC 1 cut(s) 1067
MmeI TCCRAC 3 cut(s) 122, 262, 623
MnlI CCTC 9 cut(s) 126, 137, 254, 267, 375, 464, 546, 549, 772
MroNI GCCGGC 1 cut(s) 608
MroXI GAANNNNTTC 1 cut(s) 943
MseI TTAA 4 cut(s) 141, 495, 798, 1067
MslI CAYNNNNRTG 1 cut(s) 32
MspA1I CMGCKG 1 cut(s) 428
MspI CCGG 3 cut(s) 609, 842, 1088
MspR9I CCNGG 1 cut(s) 344
MunI CAATTG 1 cut(s) 273
Mva1269I GAATGC 1 cut(s) 582
MvaI CCWGG 1 cut(s) 344
MvnI CGCG 1 cut(s) 789
MwoI GCNNNNNNNGC 1 cut(s) 293
NaeI GCCGGC 1 cut(s) 610
NdeI CATATG 1 cut(s) 302
NdeII GATC 4 cut(s) 312, 1078, 1082, 1090
NgoMIV GCCGGC 1 cut(s) 608
NlaIII CATG 5 cut(s) 37, 378, 682, 687, 880
NlaIV GGNNCC 2 cut(s) 829, 840
NsbI TGCGCA 1 cut(s) 379
NspI RCATGY 2 cut(s) 37, 378
PaeR7I CTCGAG 1 cut(s) 245
PciSI GCTCTTC 1 cut(s) 613
PcsI WCGNNNNNNNCGW 1 cut(s) 235
PctI GAATGC 1 cut(s) 582
PdiI GCCGGC 1 cut(s) 610
PdmI GAANNNNTTC 1 cut(s) 943
PfeI GAWTC 2 cut(s) 732, 967
PkrI GCNGC 2 cut(s) 427, 714
PleI GAGTC 1 cut(s) 1067
PmaCI CACGTG 1 cut(s) 776
PmlI CACGTG 1 cut(s) 776
PpsI GAGTC 1 cut(s) 1067
Ppu21I YACGTR 2 cut(s) 776, 988
PshBI ATTAAT 1 cut(s) 1067
Psp6I CCWGG 1 cut(s) 342
PspCI CACGTG 1 cut(s) 776
PspFI CCCAGC 1 cut(s) 972
PspGI CCWGG 1 cut(s) 342
PspN4I GGNNCC 2 cut(s) 829, 840
PspPI GGNCC 1 cut(s) 649
PspXI VCTCGAGB 1 cut(s) 245
PstNI CAGNNNCTG 1 cut(s) 466
RsaI GTAC 2 cut(s) 390, 962
RsaNI GTAC 2 cut(s) 389, 961
RseI CAYNNNNRTG 1 cut(s) 32
SapI GCTCTTC 1 cut(s) 613
SaqAI TTAA 4 cut(s) 141, 495, 798, 1067
SatI GCNGC 2 cut(s) 426, 713
Sau3AI GATC 4 cut(s) 312, 1078, 1082, 1090
Sau96I GGNCC 1 cut(s) 649
SchI GAGTC 1 cut(s) 1067
ScrFI CCNGG 1 cut(s) 344
SduI GDGCHC 2 cut(s) 454, 987
SetI ASST 8 cut(s) 55, 88, 137, 323, 348, 699, 778, 990
SfaNI GCATC 3 cut(s) 681, 831, 1028
Sfr274I CTCGAG 1 cut(s) 245
SlaI CTCGAG 1 cut(s) 245
SmiMI CAYNNNNRTG 1 cut(s) 32
SmlI CTYRAG 2 cut(s) 245, 322
SmoI CTYRAG 2 cut(s) 245, 322
SpeI ACTAGT 1 cut(s) 556
Sse9I AATT 4 cut(s) 122, 273, 570, 1064
SsiI CCGC 2 cut(s) 428, 787
SspI AATATT 1 cut(s) 669
SspMI CTAG 3 cut(s) 318, 557, 716
StyD4I CCNGG 1 cut(s) 342
TaaI ACNGT 1 cut(s) 292
TaiI ACGT 2 cut(s) 778, 990
TaqI TCGA 7 cut(s) 90, 246, 508, 947, 1044, 1071, 1081
TasI AATT 4 cut(s) 122, 273, 570, 1064
TatI WGTACW 1 cut(s) 388
TfiI GAWTC 2 cut(s) 732, 967
Tru1I TTAA 4 cut(s) 141, 495, 798, 1067
Tru9I TTAA 4 cut(s) 141, 495, 798, 1067
TscAI CASTG 4 cut(s) 274, 314, 532, 631
TseI GCWGC 2 cut(s) 425, 712
TspDTI ATGAA 6 cut(s) 45, 160, 289, 646, 758, 893
TspGWI ACGGA 1 cut(s) 49
TspRI CASTG 4 cut(s) 274, 314, 532, 631
VneI GTGCAC 1 cut(s) 983
VspI ATTAAT 1 cut(s) 1067
XceI RCATGY 2 cut(s) 37, 378
XhoI CTCGAG 1 cut(s) 245
XmnI GAANNNNTTC 1 cut(s) 943
XspI CTAG 3 cut(s) 318, 557, 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.