AT3G06270

protein phosphatase 2C 35

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007949
Physical Location & Seq
Forward (+)
1895858 .. 1898061
2204 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G06270.1

Sequence Viewer

Length: 1047 bp
ATGGGTTGTGTTCAATGCAAATGTTGTAGCCGATATCCTTCGTCATCATCAGATGGAGATTCTCGTGGACCTTTAGAAGCCAATGGTGTTCTTAAGGGGAAAGATCAGAAACCTCTTGGATCTATTCACGTTCCTTCTCCTAATTTCGATATGGTTTACTCTGTGTTATCTCAACGAGGCTATTACCCTGACTCACCTGATAAGGAGAATCAAGACACTTACTGCATCAAAACAGAGCTTCAAGGTAACCCGAATGTTCATTTCTTCGGTGTCTTTGATGGTCACGGTGTGTTGGGTACGCAGTGTTCGAATTTTGTTAAGGAGAGGGTTGTGGAAATGTTGTCTGAAGACCCAACTCTGCTTGAGGATCCGGAGAAAGCTTACAAGTCTGCTTTTTTGAGGGTTAATGAAGAGTTACACGATAGTGAAATCGACGATTCGATGAGTGGTACTACTGCCATTACGGTTCTTGTTGTTGGGGATAAGATTTATGTTGCCAATGTAGGAGATTCGAGGGCGGTTCTCGCTGTTAAAGACAGGAATCGGATTCTAGCAGAGGATTTGTCTTACGATCAAACGCCTTTTAGGAAAGATGAATGCGAGAGGGTTAAAGCTTGTGGAGCTAGAGTGCTGAGTGTGGATCAAGTGGAAGGGTTGAAAGATCCGAATATACAAACATGGGCAAACGAAGAGAGTGAAGGAGGAGATCCGCCGAGACTTTGGGTACAAAACGGGATGTATCCGGGAACCGCATTCACGAGGAGTGTTGGTGATTTTACAGCTGAGAGCATTGGAGTTATTGCAGAGCCAGAAGTGTCAATGGTTCATCTCTCGCCTAACCATTTGTTCTTTGTTGTTGCAAGCGATGGGATTTTCGAGTTTCTTCCAAGCCAAGCTGTTGTTGATATGGTGGGGAGATACGCAGATCCGCGCGATGGATGCGCTGCAGCAGCAGCGGAATCATATAAACTGTGGTTGGAGCATGAGAATAGGACAGATGATATCACAATTATCATTGTACAGATTAAAAAGCTGTCTAATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

348

Amino Acids

38.33

Weight (kDa)

4.69

Isoelectric Point (pI)

35.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 74 - 308 8.3e-41 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 931, 933
AccIII TCCGGA 1 cut(s) 370
AciI CCGC 5 cut(s) 518, 710, 750, 929, 956
AclWI GGATC 7 cut(s) 127, 362, 375, 648, 656, 701, 920
AcsI RAATTY 1 cut(s) 310
AcuI CTGAAG 1 cut(s) 366
AdeI CACNNNGTG 1 cut(s) 289
AfaI GTAC 4 cut(s) 298, 451, 726, 1020
AfiI CCNNNNNNNGG 1 cut(s) 935
AflII CTTAAG 1 cut(s) 92
AgsI TTSAA 3 cut(s) 14, 242, 658
AleI CACNNNNGTG 1 cut(s) 423
AluBI AGCT 7 cut(s) 238, 380, 614, 623, 782, 896, 1033
AluI AGCT 7 cut(s) 238, 380, 614, 623, 782, 896, 1033
Alw26I GTCTC 1 cut(s) 709
AlwI GGATC 7 cut(s) 127, 362, 375, 648, 656, 701, 920
Aor13HI TCCGGA 1 cut(s) 370
ApeKI GCWGC 4 cut(s) 944, 947, 950, 953
ApoI RAATTY 1 cut(s) 310
AspLEI GCGC 2 cut(s) 933, 944
AspS9I GGNCC 1 cut(s) 68
AsuC2I CCSGG 1 cut(s) 744
AsuHPI GGTGA 2 cut(s) 186, 782
AsuII TTCGAA 1 cut(s) 308
AvaII GGWCC 1 cut(s) 68
BamHI GGATCC 1 cut(s) 367
BauI CACGAG 2 cut(s) 63, 757
BbsI GAAGAC 1 cut(s) 354
BbvI GCAGC 4 cut(s) 931, 959, 962, 965
BccI CCATC 4 cut(s) 47, 272, 860, 929
BciVI GTATCC 1 cut(s) 750
BcnI CCSGG 1 cut(s) 744
BcoDI GTCTC 1 cut(s) 709
BfaI CTAG 2 cut(s) 551, 624
BfmI CTRYAG 1 cut(s) 945
BfrI CTTAAG 1 cut(s) 92
BfuI GTATCC 1 cut(s) 750
BisI GCNGC 4 cut(s) 945, 948, 951, 954
BlsI GCNGC 4 cut(s) 946, 949, 952, 955
Bme1390I CCNGG 1 cut(s) 744
Bme18I GGWCC 1 cut(s) 68
BmgT120I GGNCC 1 cut(s) 68
BmiI GGNNCC 2 cut(s) 369, 748
BmrFI CCNGG 1 cut(s) 744
BmsI GCATC 2 cut(s) 234, 929
BpiI GAAGAC 1 cut(s) 354
Bpu14I TTCGAA 1 cut(s) 308
BpuEI CTTGAG 1 cut(s) 383
BpuMI CCSGG 1 cut(s) 744
BsaWI WCCGGW 1 cut(s) 370
BsaXI ACNNNNNCTCC 4 cut(s) 612, 642, 754, 784
Bsc4I CCNNNNNNNGG 1 cut(s) 935
BseAI TCCGGA 1 cut(s) 370
BseGI GGATG 2 cut(s) 741, 944
BseLI CCNNNNNNNGG 1 cut(s) 935
BseMII CTCAG 2 cut(s) 623, 774
BseRI GAGGAG 2 cut(s) 717, 775
BseXI GCAGC 4 cut(s) 931, 959, 962, 965
Bsh1236I CGCG 2 cut(s) 931, 933
BsiSI CCGG 2 cut(s) 371, 743
BslI CCNNNNNNNGG 1 cut(s) 935
BsmAI GTCTC 1 cut(s) 709
BsmI GAATGC 2 cut(s) 602, 752
Bsp119I TTCGAA 1 cut(s) 308
Bsp13I TCCGGA 1 cut(s) 370
Bsp1407I TGTACA 1 cut(s) 1018
Bsp143I GATC 8 cut(s) 103, 119, 367, 571, 640, 661, 706, 925
BspACI CCGC 5 cut(s) 518, 710, 750, 929, 956
BspCNI CTCAG 2 cut(s) 624, 775
BspEI TCCGGA 1 cut(s) 370
BspFNI CGCG 2 cut(s) 931, 933
BspLI GGNNCC 2 cut(s) 369, 748
BspMAI CTGCAG 1 cut(s) 949
BspPI GGATC 7 cut(s) 127, 362, 375, 648, 656, 701, 920
BspT104I TTCGAA 1 cut(s) 308
BspTI CTTAAG 1 cut(s) 92
BsrGI TGTACA 1 cut(s) 1018
BssMI GATC 8 cut(s) 103, 119, 367, 571, 640, 661, 706, 925
BssSI CACGAG 2 cut(s) 63, 757
Bst2BI CACGAG 2 cut(s) 63, 757
Bst4CI ACNGT 3 cut(s) 287, 466, 972
Bst6I CTCTTC 2 cut(s) 405, 684
BstAFI CTTAAG 1 cut(s) 92
BstAUI TGTACA 1 cut(s) 1018
BstBI TTCGAA 1 cut(s) 308
BstC8I GCNNGC 1 cut(s) 862
BstDEI CTNAG 2 cut(s) 632, 783
BstEII GGTNACC 1 cut(s) 245
BstF5I GGATG 2 cut(s) 741, 944
BstFNI CGCG 2 cut(s) 931, 933
BstHHI GCGC 2 cut(s) 933, 944
BstKTI GATC 8 cut(s) 106, 122, 370, 574, 643, 664, 709, 928
BstMAI GTCTC 1 cut(s) 709
BstMBI GATC 8 cut(s) 103, 119, 367, 571, 640, 661, 706, 925
BstMWI GCNNNNNNNGC 5 cut(s) 524, 620, 939, 950, 953
BstPI GGTNACC 1 cut(s) 245
BstSCI CCNGG 1 cut(s) 742
BstSFI CTRYAG 1 cut(s) 945
BstUI CGCG 2 cut(s) 931, 933
BstV1I GCAGC 4 cut(s) 931, 959, 962, 965
BstV2I GAAGAC 1 cut(s) 354
BstX2I RGATCY 5 cut(s) 119, 367, 661, 706, 925
BstYI RGATCY 5 cut(s) 119, 367, 661, 706, 925
BsuI GTATCC 1 cut(s) 750
BtgZI GCGATG 2 cut(s) 879, 948
BtsCI GGATG 2 cut(s) 741, 944
BtsI GCAGTG 1 cut(s) 308
BtsIMutI CAGTG 1 cut(s) 308
Cac8I GCNNGC 1 cut(s) 862
CfoI GCGC 2 cut(s) 933, 944
Cfr13I GGNCC 1 cut(s) 68
Csp6I GTAC 4 cut(s) 297, 450, 725, 1019
CviAII CATG 2 cut(s) 678, 983
CviQI GTAC 4 cut(s) 297, 450, 725, 1019
DdeI CTNAG 2 cut(s) 632, 783
DpnI GATC 8 cut(s) 105, 121, 369, 573, 642, 663, 708, 927
DpnII GATC 8 cut(s) 103, 119, 367, 571, 640, 661, 706, 925
DraIII CACNNNGTG 1 cut(s) 289
Eam1104I CTCTTC 2 cut(s) 405, 684
EarI CTCTTC 2 cut(s) 405, 684
EciI GGCGGA 1 cut(s) 699
Eco32I GATATC 2 cut(s) 35, 1003
Eco47I GGWCC 1 cut(s) 68
Eco57I CTGAAG 1 cut(s) 366
Eco91I GGTNACC 1 cut(s) 245
EcoO65I GGTNACC 1 cut(s) 245
EcoRV GATATC 2 cut(s) 35, 1003
FaeI CATG 2 cut(s) 681, 986
FaiI YATR 8 cut(s) 152, 492, 671, 679, 908, 964, 966, 984
FatI CATG 2 cut(s) 677, 982
Fnu4HI GCNGC 4 cut(s) 945, 948, 951, 954
FokI GGATG 2 cut(s) 748, 951
Fsp4HI GCNGC 4 cut(s) 945, 948, 951, 954
FspBI CTAG 2 cut(s) 551, 624
GlaI GCGC 2 cut(s) 932, 943
GluI GCNGC 4 cut(s) 945, 948, 951, 954
HapII CCGG 2 cut(s) 371, 743
HhaI GCGC 2 cut(s) 933, 944
Hin1II CATG 2 cut(s) 681, 986
Hin6I GCGC 2 cut(s) 931, 942
HinP1I GCGC 2 cut(s) 931, 942
HindIII AAGCTT 2 cut(s) 378, 612
HinfI GANTC 8 cut(s) 59, 191, 208, 437, 509, 541, 547, 959
HpaII CCGG 2 cut(s) 371, 743
HphI GGTGA 2 cut(s) 186, 782
Hpy166II GTNNAC 2 cut(s) 68, 157
Hpy188I TCNGA 5 cut(s) 52, 108, 346, 546, 666
Hpy188III TCNNGA 3 cut(s) 212, 371, 757
Hpy8I GTNNAC 2 cut(s) 68, 157
Hpy99I CGWCG 1 cut(s) 437
HpyAV CCTTC 4 cut(s) 48, 144, 644, 692
HpyCH4III ACNGT 3 cut(s) 287, 466, 972
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 5 cut(s) 18, 225, 803, 860, 947
HpyF10VI GCNNNNNNNGC 5 cut(s) 524, 620, 939, 950, 953
HpyF3I CTNAG 2 cut(s) 632, 783
HpySE526I ACGT 1 cut(s) 129
Hsp92II CATG 2 cut(s) 681, 986
HspAI GCGC 2 cut(s) 931, 942
Kpn2I TCCGGA 1 cut(s) 370
Kzo9I GATC 8 cut(s) 103, 119, 367, 571, 640, 661, 706, 925
LmnI GCTCC 2 cut(s) 620, 979
LpnPI CCDG 6 cut(s) 201, 210, 384, 523, 756, 822
Lsp1109I GCAGC 4 cut(s) 931, 959, 962, 965
LweI GCATC 2 cut(s) 234, 929
MaeI CTAG 2 cut(s) 551, 624
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 3 cut(s) 245, 281, 414
MalI GATC 8 cut(s) 105, 121, 369, 573, 642, 663, 708, 927
MboI GATC 8 cut(s) 103, 119, 367, 571, 640, 661, 706, 925
MboII GAAGA 5 cut(s) 256, 359, 422, 701, 875
MflI RGATCY 5 cut(s) 119, 367, 661, 706, 925
MluCI AATT 3 cut(s) 142, 310, 1008
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 1 cut(s) 957
MroI TCCGGA 1 cut(s) 370
MseI TTAA 6 cut(s) 93, 318, 405, 531, 609, 1026
MslI CAYNNNNRTG 1 cut(s) 423
MspA1I CMGCKG 2 cut(s) 782, 956
MspCI CTTAAG 1 cut(s) 92
MspI CCGG 2 cut(s) 371, 743
MspR9I CCNGG 1 cut(s) 744
Mva1269I GAATGC 2 cut(s) 602, 752
MvnI CGCG 2 cut(s) 931, 933
MwoI GCNNNNNNNGC 5 cut(s) 524, 620, 939, 950, 953
NciI CCSGG 1 cut(s) 744
NdeII GATC 8 cut(s) 103, 119, 367, 571, 640, 661, 706, 925
NlaIII CATG 2 cut(s) 681, 986
NlaIV GGNNCC 2 cut(s) 369, 748
NmeAIII GCCGAG 1 cut(s) 738
NmuCI GTSAC 1 cut(s) 281
NspV TTCGAA 1 cut(s) 308
OliI CACNNNNGTG 1 cut(s) 423
PcsI WCGNNNNNNNCGW 1 cut(s) 305
PctI GAATGC 2 cut(s) 602, 752
PfeI GAWTC 7 cut(s) 59, 208, 437, 509, 541, 547, 959
PfoI TCCNGGA 1 cut(s) 742
PkrI GCNGC 4 cut(s) 946, 949, 952, 955
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
PspEI GGTNACC 1 cut(s) 245
PspN4I GGNNCC 2 cut(s) 369, 748
PspPI GGNCC 1 cut(s) 68
PsrI GAACNNNNNNTAC 2 cut(s) 289, 321
PstI CTGCAG 1 cut(s) 949
PsuI RGATCY 5 cut(s) 119, 367, 661, 706, 925
PvuII CAGCTG 1 cut(s) 782
RsaI GTAC 4 cut(s) 298, 451, 726, 1020
RsaNI GTAC 4 cut(s) 297, 450, 725, 1019
RseI CAYNNNNRTG 1 cut(s) 423
SaqAI TTAA 6 cut(s) 93, 318, 405, 531, 609, 1026
SatI GCNGC 4 cut(s) 945, 948, 951, 954
Sau3AI GATC 8 cut(s) 103, 119, 367, 571, 640, 661, 706, 925
Sau96I GGNCC 1 cut(s) 68
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 1 cut(s) 744
SfaNI GCATC 2 cut(s) 234, 929
SfcI CTRYAG 1 cut(s) 945
SfuI TTCGAA 1 cut(s) 308
SinI GGWCC 1 cut(s) 68
SmiMI CAYNNNNRTG 1 cut(s) 423
SmlI CTYRAG 2 cut(s) 92, 362
SmoI CTYRAG 2 cut(s) 92, 362
Sse9I AATT 3 cut(s) 142, 310, 1008
SsiI CCGC 5 cut(s) 518, 710, 750, 929, 956
SspMI CTAG 2 cut(s) 551, 624
StyD4I CCNGG 1 cut(s) 742
TaaI ACNGT 3 cut(s) 287, 466, 972
TaiI ACGT 1 cut(s) 132
TaqI TCGA 6 cut(s) 147, 308, 432, 440, 512, 876
TasI AATT 3 cut(s) 142, 310, 1008
TatI WGTACW 1 cut(s) 1018
TfiI GAWTC 7 cut(s) 59, 208, 437, 509, 541, 547, 959
Tru1I TTAA 6 cut(s) 93, 318, 405, 531, 609, 1026
Tru9I TTAA 6 cut(s) 93, 318, 405, 531, 609, 1026
TscAI CASTG 1 cut(s) 308
TseFI GTSAC 1 cut(s) 281
TseI GCWGC 4 cut(s) 944, 947, 950, 953
Tsp45I GTSAC 1 cut(s) 281
TspDTI ATGAA 4 cut(s) 248, 423, 609, 815
TspRI CASTG 1 cut(s) 308
Vha464I CTTAAG 1 cut(s) 92
VpaK11BI GGWCC 1 cut(s) 68
XapI RAATTY 1 cut(s) 310
XspI CTAG 2 cut(s) 551, 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.