pycom13g15430

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007949
Physical Location & Seq
Reverse (-)
11241579 .. 11244268
2690 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g15430.2

Sequence Viewer

Length: 1278 bp
ATGGGTTGTGTCCATGGCAAGTGTTGTGGCCGTTACCCCCAGTCTTCCGTGATGGTCGATCTCCGGGACTTCAGAGATGCTGGTCCGTACAGCGCTCAGAGGAAGCATATACTCTCACAGAGGTCATTGGAGTTCGTCCCTGTTCCTTCACACGGCTATCGTTTGGAGTACTCTTTGTTAACACAGCGCGGCTACTATCCTGACTCGCCGGATAAGGAAAACCAGGACAGTTTCTGTGTTAGTAAGCAAGTCCGAGGTAACCCGAATATCCATTTCTTTGGTGTGTTTGATGGGCATGGCGAACTTGGTACCCAGTGTGCTAATTTTGTTAAGGACAGGTTAATAGAGATATTAGCCAATGATTCCACATTGTCAGCTGACCCTGTGAAATCTTACAATTCTGCTTTTCTAACGACGAATTATGAGTTGCATAATAGTAAGATTGATGATACTATGAGTGGTACCACGGCAATTACTGTGCTTGTTATTGGGGATACCCTTTATGTCGCGAATGTGGGTGATTCGAGAGCAGTGATTGCAGTTAAAGATGGTAATAGAATTATTGCAGAGGACTTGTCTTGTGACCAAACGCCATTTAGGAAAGATGAGTATGAGAGAGTGAAGCTGTGTGGGGCCAGAGTTATGAGTGTTGATCAAGTGGAGGGGCTGAAGGATCCAGATATCCAGACGTGGGGTGATGAAGAGAGTGAAAGTGGTGATCCTCCAAGGTTGTGGATTCCAAATGGAAGGTATCCTGGAACTGCGTTTACAAGGAGTGTAGGTGATAGTACCGCTGAGACGATTGGTGTGGTTGCCACTCCAGAGGTTTCAATGGTTAAGCTGACACCTAATCATCTCTTCTTTGTTGTCGCAAGTGATGGAGTTTTCGAGTTCCTCTCAAGCCAAGCTGTGGTTGATATGGCGGCAAGATATCCAGATCGTCGAGATGCATGTGCTGCCATTGCTGGAGAATCATACAAATTATGGTTAGAACATGAAATCCGGACAGATGATATAACAATAATCATTGTTCACATCAAAGGCTTGTCCAATTCAGCTGGTGGTGCTACAGATGGAGCTAGTGGGGCCAATAATAGGCCAGCAACAAGGGTGAGAAAAATTTCTGAGTCATCTGGAACCACTGGGTCGGAAGTTTACCGTTCTGTGAGAAGTGATTCCTCAGAAATACAGTCTTGTCAGCTTGTGCTTTCGATGAATCGAAGCCCAGCTATTGTTGTTCCATCTCCTACATGTCAGAGCCCTGTGAGTTGCGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

426

Amino Acids

46.33

Weight (kDa)

5.29

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 308, 461
AccB1I GGYRCC 2 cut(s) 308, 461
AccB7I CCANNNNNTGG 1 cut(s) 910
AccII CGCG 2 cut(s) 189, 509
AccIII TCCGGA 1 cut(s) 1002
AciI CCGC 4 cut(s) 189, 792, 923, 1272
AclWI GGATC 3 cut(s) 668, 681, 713
AcoI YGGCCR 1 cut(s) 28
AcsI RAATTY 1 cut(s) 1119
AcuI CTGAAG 2 cut(s) 55, 689
AfaI GTAC 5 cut(s) 89, 170, 310, 463, 790
AfeI AGCGCT 1 cut(s) 94
AfiI CCNNNNNNNGG 4 cut(s) 152, 691, 910, 1095
AflIII ACRYGT 1 cut(s) 1250
AgsI TTSAA 1 cut(s) 831
AjiI CACGTC 1 cut(s) 690
AjnI CCWGG 2 cut(s) 222, 754
AloI GAACNNNNNNTCC 2 cut(s) 984, 1016
AluBI AGCT 8 cut(s) 377, 625, 841, 908, 1058, 1079, 1201, 1229
AluI AGCT 8 cut(s) 377, 625, 841, 908, 1058, 1079, 1201, 1229
Alw26I GTCTC 1 cut(s) 791
AlwI GGATC 3 cut(s) 668, 681, 713
AlwNI CAGNNNCTG 1 cut(s) 234
Aor13HI TCCGGA 1 cut(s) 1002
Aor51HI AGCGCT 1 cut(s) 94
AoxI GGCC 4 cut(s) 28, 633, 1086, 1097
ApeKI GCWGC 1 cut(s) 956
ApoI RAATTY 1 cut(s) 1119
Asp700I GAANNNNTTC 2 cut(s) 1120, 1174
Asp718I GGTACC 2 cut(s) 308, 461
AspLEI GCGC 2 cut(s) 95, 189
AspS9I GGNCC 3 cut(s) 83, 633, 1086
AsuC2I CCSGG 1 cut(s) 65
AsuHPI GGTGA 5 cut(s) 530, 707, 728, 794, 1123
AvaII GGWCC 1 cut(s) 83
BamHI GGATCC 1 cut(s) 673
BanI GGYRCC 2 cut(s) 308, 461
BanII GRGCYC 1 cut(s) 1262
BbsI GAAGAC 1 cut(s) 36
BbvI GCAGC 1 cut(s) 943
BccI CCATC 6 cut(s) 46, 284, 542, 872, 1067, 1249
BceAI ACGGC 3 cut(s) 15, 169, 483
BciT130I CCWGG 2 cut(s) 224, 756
BciVI GTATCC 2 cut(s) 487, 762
BclI TGATCA 1 cut(s) 652
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 1 cut(s) 791
BfaI CTAG 1 cut(s) 1080
BfmI CTRYAG 1 cut(s) 1068
BfoI RGCGCY 1 cut(s) 96
BfuI GTATCC 2 cut(s) 487, 762
BisI GCNGC 3 cut(s) 190, 924, 957
BlsI GCNGC 3 cut(s) 191, 925, 958
BmcAI AGTACT 1 cut(s) 170
Bme1390I CCNGG 3 cut(s) 65, 224, 756
Bme18I GGWCC 1 cut(s) 83
BmgBI CACGTC 1 cut(s) 690
BmgT120I GGNCC 3 cut(s) 83, 633, 1086
BmiI GGNNCC 6 cut(s) 310, 463, 634, 675, 1087, 1138
BmrFI CCNGG 3 cut(s) 65, 224, 756
BmrI ACTGGG 3 cut(s) 34, 307, 1152
BmsI GCATC 2 cut(s) 67, 937
BmuI ACTGGG 3 cut(s) 34, 307, 1152
BpiI GAAGAC 1 cut(s) 36
BplI GAGNNNNNCTC 2 cut(s) 881, 913
BpmI CTGGAG 2 cut(s) 804, 987
BpuEI CTTGAG 1 cut(s) 883
BpuMI CCSGG 1 cut(s) 65
BsaJI CCNNGG 4 cut(s) 13, 253, 465, 725
BsaWI WCCGGW 1 cut(s) 1002
BsaXI ACNNNNNCTCC 2 cut(s) 766, 796
Bsc4I CCNNNNNNNGG 4 cut(s) 152, 691, 910, 1095
Bse1I ACTGG 3 cut(s) 40, 313, 1147
Bse3DI GCAATG 1 cut(s) 960
BseAI TCCGGA 1 cut(s) 1002
BseBI CCWGG 2 cut(s) 224, 756
BseDI CCNNGG 4 cut(s) 13, 253, 465, 725
BseLI CCNNNNNNNGG 4 cut(s) 152, 691, 910, 1095
BseMI GCAATG 1 cut(s) 960
BseMII CTCAG 4 cut(s) 110, 786, 1116, 1194
BseNI ACTGG 3 cut(s) 40, 313, 1147
BseXI GCAGC 1 cut(s) 943
BseYI CCCAGC 1 cut(s) 1225
Bsh1236I CGCG 2 cut(s) 189, 509
BshFI GGCC 4 cut(s) 30, 635, 1088, 1099
BshNI GGYRCC 2 cut(s) 308, 461
BsiSI CCGG 3 cut(s) 64, 209, 1003
BslFI GGGAC 2 cut(s) 80, 122
BslI CCNNNNNNNGG 4 cut(s) 152, 691, 910, 1095
BsmAI GTCTC 1 cut(s) 791
BsmBI CGTCTC 1 cut(s) 791
BsmFI GGGAC 2 cut(s) 80, 122
BsnI GGCC 4 cut(s) 30, 635, 1088, 1099
Bsp1286I GDGCHC 1 cut(s) 1262
Bsp13I TCCGGA 1 cut(s) 1002
Bsp143I GATC 5 cut(s) 58, 652, 673, 718, 937
Bsp19I CCATGG 1 cut(s) 13
Bsp68I TCGCGA 1 cut(s) 509
BspACI CCGC 4 cut(s) 189, 792, 923, 1272
BspANI GGCC 4 cut(s) 30, 635, 1088, 1099
BspCNI CTCAG 4 cut(s) 109, 787, 1117, 1193
BspEI TCCGGA 1 cut(s) 1002
BspFNI CGCG 2 cut(s) 189, 509
BspLI GGNNCC 6 cut(s) 310, 463, 634, 675, 1087, 1138
BspPI GGATC 3 cut(s) 668, 681, 713
BspT107I GGYRCC 2 cut(s) 308, 461
BsrDI GCAATG 1 cut(s) 960
BsrI ACTGG 3 cut(s) 40, 313, 1147
BssECI CCNNGG 4 cut(s) 13, 253, 465, 725
BssMI GATC 5 cut(s) 58, 652, 673, 718, 937
BssT1I CCWWGG 2 cut(s) 13, 725
Bst2UI CCWGG 2 cut(s) 224, 756
Bst4CI ACNGT 4 cut(s) 230, 478, 1160, 1191
Bst6I CTCTTC 2 cut(s) 696, 863
BstAPI GCANNNNNTGC 2 cut(s) 536, 956
BstC8I GCNNGC 1 cut(s) 1101
BstDEI CTNAG 4 cut(s) 96, 795, 1125, 1180
BstDSI CCRYGG 2 cut(s) 13, 465
BstEII GGTNACC 1 cut(s) 257
BstFNI CGCG 2 cut(s) 189, 509
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 2 cut(s) 95, 189
BstKTI GATC 5 cut(s) 61, 655, 676, 721, 940
BstMAI GTCTC 1 cut(s) 791
BstMBI GATC 5 cut(s) 58, 652, 673, 718, 937
BstMWI GCNNNNNNNGC 5 cut(s) 536, 956, 962, 1064, 1085
BstNI CCWGG 2 cut(s) 224, 756
BstNSI RCATGY 2 cut(s) 954, 1254
BstPI GGTNACC 1 cut(s) 257
BstSCI CCNGG 3 cut(s) 63, 222, 754
BstSFI CTRYAG 1 cut(s) 1068
BstUI CGCG 2 cut(s) 189, 509
BstV1I GCAGC 1 cut(s) 943
BstV2I GAAGAC 1 cut(s) 36
BstX2I RGATCY 1 cut(s) 673
BstXI CCANNNNNNTGG 2 cut(s) 278, 732
BstYI RGATCY 1 cut(s) 673
BsuI GTATCC 2 cut(s) 487, 762
BsuRI GGCC 4 cut(s) 30, 635, 1088, 1099
BtgI CCRYGG 2 cut(s) 13, 465
BtrI CACGTC 1 cut(s) 690
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 3 cut(s) 320, 537, 1140
BtuMI TCGCGA 1 cut(s) 509
Cac8I GCNNGC 1 cut(s) 1101
CaiI CAGNNNCTG 1 cut(s) 234
CfoI GCGC 2 cut(s) 95, 189
Cfr13I GGNCC 3 cut(s) 83, 633, 1086
Csp6I GTAC 5 cut(s) 88, 169, 309, 462, 789
CspCI CAANNNNNGTGG 2 cut(s) 7, 42
CviAII CATG 5 cut(s) 14, 296, 951, 995, 1251
CviQI GTAC 5 cut(s) 88, 169, 309, 462, 789
DdeI CTNAG 4 cut(s) 96, 795, 1125, 1180
DpnI GATC 5 cut(s) 60, 654, 675, 720, 939
DpnII GATC 5 cut(s) 58, 652, 673, 718, 937
EaeI YGGCCR 1 cut(s) 28
Eam1104I CTCTTC 2 cut(s) 696, 863
EarI CTCTTC 2 cut(s) 696, 863
Eco130I CCWWGG 2 cut(s) 13, 725
Eco24I GRGCYC 1 cut(s) 1262
Eco32I GATATC 2 cut(s) 682, 932
Eco47I GGWCC 1 cut(s) 83
Eco47III AGCGCT 1 cut(s) 94
Eco57I CTGAAG 2 cut(s) 55, 689
Eco91I GGTNACC 1 cut(s) 257
EcoO65I GGTNACC 1 cut(s) 257
EcoRII CCWGG 2 cut(s) 222, 754
EcoRV GATATC 2 cut(s) 682, 932
EcoT14I CCWWGG 2 cut(s) 13, 725
EcoT22I ATGCAT 1 cut(s) 952
EcoT38I GRGCYC 1 cut(s) 1262
ErhI CCWWGG 2 cut(s) 13, 725
Esp3I CGTCTC 1 cut(s) 791
FaeI CATG 5 cut(s) 17, 299, 954, 998, 1254
FaqI GGGAC 2 cut(s) 80, 122
FatI CATG 5 cut(s) 13, 295, 950, 994, 1250
FbaI TGATCA 1 cut(s) 652
Fnu4HI GCNGC 3 cut(s) 190, 924, 957
FriOI GRGCYC 1 cut(s) 1262
Fsp4HI GCNGC 3 cut(s) 190, 924, 957
FspBI CTAG 1 cut(s) 1080
GlaI GCGC 2 cut(s) 94, 188
GluI GCNGC 3 cut(s) 190, 924, 957
GsaI CCCAGC 1 cut(s) 1229
GsuI CTGGAG 2 cut(s) 804, 987
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 4 cut(s) 30, 635, 1088, 1099
HapII CCGG 3 cut(s) 64, 209, 1003
HhaI GCGC 2 cut(s) 95, 189
Hin1II CATG 5 cut(s) 17, 299, 954, 998, 1254
Hin6I GCGC 2 cut(s) 93, 187
HinP1I GCGC 2 cut(s) 93, 187
HincII GTYRAC 1 cut(s) 180
HindII GTYRAC 1 cut(s) 180
HinfI GANTC 8 cut(s) 203, 362, 521, 736, 971, 1127, 1175, 1216
HpaI GTTAAC 1 cut(s) 180
HpaII CCGG 3 cut(s) 64, 209, 1003
HphI GGTGA 5 cut(s) 530, 707, 728, 794, 1123
Hpy166II GTNNAC 4 cut(s) 180, 768, 1033, 1156
Hpy188I TCNGA 7 cut(s) 74, 99, 254, 1126, 1150, 1183, 1257
Hpy8I GTNNAC 4 cut(s) 180, 768, 1033, 1156
Hpy99I CGWCG 2 cut(s) 418, 945
HpyAV CCTTC 3 cut(s) 156, 664, 741
HpyCH4III ACNGT 4 cut(s) 230, 478, 1160, 1191
HpyCH4IV ACGT 1 cut(s) 689
HpyCH4V TGCA 4 cut(s) 430, 539, 566, 950
HpyF10VI GCNNNNNNNGC 5 cut(s) 536, 956, 962, 1064, 1085
HpyF3I CTNAG 4 cut(s) 96, 795, 1125, 1180
HpySE526I ACGT 1 cut(s) 689
Hsp92II CATG 5 cut(s) 17, 299, 954, 998, 1254
HspAI GCGC 2 cut(s) 93, 187
Kpn2I TCCGGA 1 cut(s) 1002
KpnI GGTACC 2 cut(s) 312, 465
Ksp22I TGATCA 1 cut(s) 652
KspAI GTTAAC 1 cut(s) 180
Kzo9I GATC 5 cut(s) 58, 652, 673, 718, 937
LmnI GCTCC 1 cut(s) 1076
Lsp1109I GCAGC 1 cut(s) 943
LweI GCATC 2 cut(s) 67, 937
MaeI CTAG 1 cut(s) 1080
MaeII ACGT 1 cut(s) 689
MaeIII GTNAC 3 cut(s) 32, 257, 581
MalI GATC 5 cut(s) 60, 654, 675, 720, 939
MboI GATC 5 cut(s) 58, 652, 673, 718, 937
MboII GAAGA 3 cut(s) 36, 713, 850
MflI RGATCY 1 cut(s) 673
MhlI GDGCHC 1 cut(s) 1262
MluCI AATT 8 cut(s) 322, 397, 418, 471, 558, 980, 1051, 1119
MlyI GAGTC 2 cut(s) 197, 1136
MmeI TCCRAC 1 cut(s) 1128
MnlI CCTC 9 cut(s) 93, 114, 248, 562, 655, 732, 817, 905, 1189
Mph1103I ATGCAT 1 cut(s) 952
MroI TCCGGA 1 cut(s) 1002
MroXI GAANNNNTTC 2 cut(s) 1120, 1174
MseI TTAA 5 cut(s) 179, 330, 341, 543, 837
MspA1I CMGCKG 3 cut(s) 377, 794, 1058
MspI CCGG 3 cut(s) 64, 209, 1003
MspR9I CCNGG 3 cut(s) 65, 224, 756
MvaI CCWGG 2 cut(s) 224, 756
MvnI CGCG 2 cut(s) 189, 509
MwoI GCNNNNNNNGC 5 cut(s) 536, 956, 962, 1064, 1085
NciI CCSGG 1 cut(s) 65
NcoI CCATGG 1 cut(s) 13
NdeII GATC 5 cut(s) 58, 652, 673, 718, 937
NlaIII CATG 5 cut(s) 17, 299, 954, 998, 1254
NlaIV GGNNCC 6 cut(s) 310, 463, 634, 675, 1087, 1138
NmuCI GTSAC 1 cut(s) 581
NruI TCGCGA 1 cut(s) 509
NsiI ATGCAT 1 cut(s) 952
NspI RCATGY 2 cut(s) 954, 1254
PciI ACATGT 1 cut(s) 1250
PdmI GAANNNNTTC 2 cut(s) 1120, 1174
PfeI GAWTC 6 cut(s) 362, 521, 736, 971, 1175, 1216
PflMI CCANNNNNTGG 1 cut(s) 910
PfoI TCCNGGA 2 cut(s) 63, 754
PkrI GCNGC 3 cut(s) 191, 925, 958
PleI GAGTC 2 cut(s) 197, 1135
PpsI GAGTC 2 cut(s) 197, 1135
PscI ACATGT 1 cut(s) 1250
Psp6I CCWGG 2 cut(s) 222, 754
PspEI GGTNACC 1 cut(s) 257
PspFI CCCAGC 1 cut(s) 1225
PspGI CCWGG 2 cut(s) 222, 754
PspN4I GGNNCC 6 cut(s) 310, 463, 634, 675, 1087, 1138
PspPI GGNCC 3 cut(s) 83, 633, 1086
PsrI GAACNNNNNNTAC 2 cut(s) 751, 783
PstNI CAGNNNCTG 1 cut(s) 234
PsuI RGATCY 1 cut(s) 673
PvuII CAGCTG 2 cut(s) 377, 1058
RruI TCGCGA 1 cut(s) 509
RsaI GTAC 5 cut(s) 89, 170, 310, 463, 790
RsaNI GTAC 5 cut(s) 88, 169, 309, 462, 789
SaqAI TTAA 5 cut(s) 179, 330, 341, 543, 837
SatI GCNGC 3 cut(s) 190, 924, 957
Sau3AI GATC 5 cut(s) 58, 652, 673, 718, 937
Sau96I GGNCC 3 cut(s) 83, 633, 1086
ScaI AGTACT 1 cut(s) 170
SchI GAGTC 2 cut(s) 197, 1136
ScrFI CCNGG 3 cut(s) 65, 224, 756
SduI GDGCHC 1 cut(s) 1262
SfaNI GCATC 2 cut(s) 67, 937
SfcI CTRYAG 1 cut(s) 1068
SinI GGWCC 1 cut(s) 83
SmlI CTYRAG 1 cut(s) 898
SmoI CTYRAG 1 cut(s) 898
Sse9I AATT 8 cut(s) 322, 397, 418, 471, 558, 980, 1051, 1119
SsiI CCGC 4 cut(s) 189, 792, 923, 1272
SspMI CTAG 1 cut(s) 1080
StyD4I CCNGG 3 cut(s) 63, 222, 754
StyI CCWWGG 2 cut(s) 13, 725
TaaI ACNGT 4 cut(s) 230, 478, 1160, 1191
TaiI ACGT 1 cut(s) 692
TaqI TCGA 6 cut(s) 57, 524, 888, 943, 1211, 1219
TasI AATT 8 cut(s) 322, 397, 418, 471, 558, 980, 1051, 1119
TatI WGTACW 1 cut(s) 168
TauI GCSGC 2 cut(s) 192, 926
TfiI GAWTC 6 cut(s) 362, 521, 736, 971, 1175, 1216
Tru1I TTAA 5 cut(s) 179, 330, 341, 543, 837
Tru9I TTAA 5 cut(s) 179, 330, 341, 543, 837
TscAI CASTG 3 cut(s) 320, 537, 1147
TseFI GTSAC 1 cut(s) 581
TseI GCWGC 1 cut(s) 956
Tsp45I GTSAC 1 cut(s) 581
TspDTI ATGAA 3 cut(s) 714, 1011, 1229
TspGWI ACGGA 2 cut(s) 37, 75
TspRI CASTG 3 cut(s) 320, 537, 1147
Van91I CCANNNNNTGG 1 cut(s) 910
VpaK11BI GGWCC 1 cut(s) 83
XapI RAATTY 1 cut(s) 1119
XceI RCATGY 2 cut(s) 954, 1254
XmnI GAANNNNTTC 2 cut(s) 1120, 1174
XspI CTAG 1 cut(s) 1080
ZrmI AGTACT 1 cut(s) 170
Zsp2I ATGCAT 1 cut(s) 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.