Rroxscaffold_5G00362550

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0007949
Physical Location & Seq
Forward (+)
43578968 .. 43582370
3403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00362550.1

Sequence Viewer

Length: 1242 bp
ATGGGTTGCGTCCATGGCAAGTGTTGCGGTCGTTTTCCCAAGTCCTCCGACGGCGATCCCCGGGAATTCAGAGACTCCGGACCCTACAGCGCTCAGAGGAAGCAGCACATACTCACAGAGAGGTCATTGGAGTTGGTCCCCGTTCCTTCACACGGCTTCAATTTGGAGTACACTGTCCTAACGCAGAGGGGTTACTATCCCGACTCGCCGGATAAGGATAATCAGGACAGTTTCTGTGTTAAAACCCAGCTTCAAGGTAACCCAAATATCCATTTCTTTGGTGTGTTTGATGGGCATGGTCAGTTTGGTACCCAGTGTGCTAATCTTGTTAAGGATAGACTGGTAGAGGTGTTAGCAGATGATCCCACATTGCCTGATGACCCTGTGGTAGCTTATAATTCTGCTTTTTTAACCACGAATAGCGAGTTGCATAGTAGTGTGATTGATGATACTATGAGTGGTACCACGGCGATTACTGTGCTTGTGGTTGGGAATACCCTGTATGTTGCGAATGTGGGTGATTCGAGAGCAGTGATTGCGGTGAAAGATGGTAATAGGATTATTGCGGAGGATTTGTCTTGTGACCAAACGCCGTTTAGGAAAGATGAGTATGAGAGAGTGAAGCAGTCTGGAGCCAGGGTTTTGAGTGTTGATCAAGTGGAAGGGCTTAAGGATCCAAGCATTCAGAGTTGGGGTGATGAAGAGAGTGAAGGGGTGACCCTCCAAGGCTGTGGGTTCAAAATGCTGACTCCCAATCATCTTTTCTTTGTCGTTGCAAGTGATGGGGTTTTCGAGTTCCTCTCAAGCCAAGCTGTTGTTAATATGGCAGCAAAATATTCAGATCCTCGGGATGCATGTGCTGCCATTGCTGGAGAATCATATAAGTTATGGTTGGAACATGAAAACCGAACAGATGATATAACAATTATCATTGTTCACATCAAAGGCTTGTCTAATTCAGCTGGTGGTGCTACAGATGGAACAGATGGGAGCACTAATAGAGCTGCAAGGAGGAGGAGAAAAGGGAATTCTGGGTCATCATTTAACAGCGGTTCGGAAGTTTTCCGTTCAGTGAGAAGTGATTCCTCAGAGATACAGTCTTGTCAGCTCGTGCTTTCGATGAATCGAAACCCAGCTATTGTTGTTCCATCTCCTACATGTCAAAGCCCCAGAAATAGGATGCGGGTTAGTGGTGCAATTCAACCAACAACAGAAGCAGTTGGTTCTGCATATACTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

413

Amino Acids

44.85

Weight (kDa)

5.35

Isoelectric Point (pI)

43.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 78 - 222 4.3e-24 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 396
Acc65I GGTACC 2 cut(s) 308, 461
AccB1I GGYRCC 2 cut(s) 308, 461
AccIII TCCGGA 1 cut(s) 77
AciI CCGC 5 cut(s) 27, 539, 566, 1050, 1183
AclWI GGATC 5 cut(s) 50, 356, 668, 681, 836
AcsI RAATTY 2 cut(s) 65, 1027
AfaI GTAC 3 cut(s) 170, 310, 463
AfeI AGCGCT 1 cut(s) 91
AfiI CCNNNNNNNGG 2 cut(s) 152, 1176
AflII CTTAAG 1 cut(s) 668
AflIII ACRYGT 1 cut(s) 1157
AgsI TTSAA 4 cut(s) 160, 254, 739, 1202
AjnI CCWGG 1 cut(s) 635
AjuI GAANNNNNNNTTGG 2 cut(s) 746, 778
AluBI AGCT 7 cut(s) 250, 392, 812, 962, 1004, 1108, 1136
AluI AGCT 7 cut(s) 250, 392, 812, 962, 1004, 1108, 1136
Alw21I GWGCWC 1 cut(s) 995
Alw26I GTCTC 1 cut(s) 66
AlwI GGATC 5 cut(s) 50, 356, 668, 681, 836
AlwNI CAGNNNCTG 1 cut(s) 234
Ama87I CYCGRG 2 cut(s) 60, 846
Aor13HI TCCGGA 1 cut(s) 77
Aor51HI AGCGCT 1 cut(s) 91
ApeKI GCWGC 4 cut(s) 103, 827, 860, 1004
ApoI RAATTY 2 cut(s) 65, 1027
Asp700I GAANNNNTTC 1 cut(s) 1081
Asp718I GGTACC 2 cut(s) 308, 461
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 2 cut(s) 80, 136
AsuC2I CCSGG 2 cut(s) 61, 62
AsuHPI GGTGA 4 cut(s) 530, 553, 707, 727
AvaI CYCGRG 2 cut(s) 60, 846
AvaII GGWCC 2 cut(s) 80, 136
BamHI GGATCC 1 cut(s) 673
BanI GGYRCC 2 cut(s) 308, 461
BauI CACGAG 1 cut(s) 1109
Bbv12I GWGCWC 1 cut(s) 995
BbvI GCAGC 4 cut(s) 115, 839, 847, 991
BccI CCATC 6 cut(s) 284, 542, 776, 971, 980, 1156
BceAI ACGGC 4 cut(s) 67, 169, 483, 577
BcgI CGANNNNNNTGC 2 cut(s) 460, 494
BciT130I CCWGG 1 cut(s) 637
BclI TGATCA 1 cut(s) 652
BcnI CCSGG 2 cut(s) 61, 62
BcoDI GTCTC 1 cut(s) 66
BfmI CTRYAG 2 cut(s) 85, 972
BfoI RGCGCY 1 cut(s) 93
BfrI CTTAAG 1 cut(s) 668
BisI GCNGC 4 cut(s) 104, 828, 861, 1005
BlsI GCNGC 4 cut(s) 105, 829, 862, 1006
Bme1390I CCNGG 3 cut(s) 61, 62, 637
Bme18I GGWCC 2 cut(s) 80, 136
BmeT110I CYCGRG 2 cut(s) 60, 846
BmgT120I GGNCC 2 cut(s) 80, 136
BmiI GGNNCC 6 cut(s) 82, 138, 310, 463, 634, 675
BmrFI CCNGG 3 cut(s) 61, 62, 637
BmrI ACTGGG 1 cut(s) 307
BmsI GCATC 2 cut(s) 841, 1170
BmuI ACTGGG 1 cut(s) 307
BplI GAGNNNNNCTC 2 cut(s) 785, 817
BpmI CTGGAG 2 cut(s) 651, 891
BpuEI CTTGAG 1 cut(s) 787
BpuMI CCSGG 2 cut(s) 61, 62
BsaJI CCNNGG 7 cut(s) 13, 59, 60, 465, 636, 724, 845
BsaWI WCCGGW 1 cut(s) 77
BsaXI ACNNNNNCTCC 4 cut(s) 560, 590, 624, 654
Bsc4I CCNNNNNNNGG 2 cut(s) 152, 1176
Bse1I ACTGG 2 cut(s) 313, 345
Bse3DI GCAATG 2 cut(s) 368, 864
BseAI TCCGGA 1 cut(s) 77
BseBI CCWGG 1 cut(s) 637
BseDI CCNNGG 7 cut(s) 13, 59, 60, 465, 636, 724, 845
BseGI GGATG 2 cut(s) 856, 1185
BseLI CCNNNNNNNGG 2 cut(s) 152, 1176
BseMI GCAATG 2 cut(s) 368, 864
BseMII CTCAG 2 cut(s) 107, 1101
BseNI ACTGG 2 cut(s) 313, 345
BseRI GAGGAG 2 cut(s) 1027, 1030
BseXI GCAGC 4 cut(s) 115, 839, 847, 991
BseYI CCCAGC 2 cut(s) 246, 1132
Bsh1285I CGRYCG 1 cut(s) 31
BshNI GGYRCC 2 cut(s) 308, 461
BsiEI CGRYCG 1 cut(s) 31
BsiHKAI GWGCWC 1 cut(s) 995
BsiHKCI CYCGRG 2 cut(s) 60, 846
BsiSI CCGG 3 cut(s) 61, 78, 209
BslFI GGGAC 1 cut(s) 122
BslI CCNNNNNNNGG 2 cut(s) 152, 1176
BsmAI GTCTC 1 cut(s) 66
BsmFI GGGAC 1 cut(s) 122
BsmI GAATGC 1 cut(s) 681
BsoBI CYCGRG 2 cut(s) 60, 846
Bsp1286I GDGCHC 1 cut(s) 995
Bsp13I TCCGGA 1 cut(s) 77
Bsp143I GATC 5 cut(s) 55, 361, 652, 673, 841
Bsp19I CCATGG 1 cut(s) 13
BspACI CCGC 5 cut(s) 27, 539, 566, 1050, 1183
BspCNI CTCAG 2 cut(s) 106, 1100
BspEI TCCGGA 1 cut(s) 77
BspLI GGNNCC 6 cut(s) 82, 138, 310, 463, 634, 675
BspPI GGATC 5 cut(s) 50, 356, 668, 681, 836
BspT107I GGYRCC 2 cut(s) 308, 461
BspTI CTTAAG 1 cut(s) 668
BsrDI GCAATG 2 cut(s) 368, 864
BsrI ACTGG 2 cut(s) 313, 345
BssECI CCNNGG 7 cut(s) 13, 59, 60, 465, 636, 724, 845
BssMI GATC 5 cut(s) 55, 361, 652, 673, 841
BssSI CACGAG 1 cut(s) 1109
BssT1I CCWWGG 2 cut(s) 13, 724
Bst2BI CACGAG 1 cut(s) 1109
Bst2UI CCWGG 1 cut(s) 637
Bst4CI ACNGT 5 cut(s) 175, 230, 478, 1098, 1237
Bst6I CTCTTC 1 cut(s) 696
BstAFI CTTAAG 1 cut(s) 668
BstAPI GCANNNNNTGC 3 cut(s) 24, 536, 860
BstDEI CTNAG 2 cut(s) 93, 1087
BstDSI CCRYGG 2 cut(s) 13, 465
BstEII GGTNACC 2 cut(s) 257, 715
BstF5I GGATG 2 cut(s) 856, 1185
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 5 cut(s) 58, 364, 655, 676, 844
BstMAI GTCTC 1 cut(s) 66
BstMBI GATC 5 cut(s) 55, 361, 652, 673, 841
BstMCI CGRYCG 1 cut(s) 31
BstMWI GCNNNNNNNGC 6 cut(s) 15, 24, 536, 860, 866, 968
BstNI CCWGG 1 cut(s) 637
BstNSI RCATGY 2 cut(s) 858, 1161
BstPI GGTNACC 2 cut(s) 257, 715
BstSCI CCNGG 3 cut(s) 59, 60, 635
BstSFI CTRYAG 2 cut(s) 85, 972
BstV1I GCAGC 4 cut(s) 115, 839, 847, 991
BstX2I RGATCY 2 cut(s) 673, 841
BstXI CCANNNNNNTGG 2 cut(s) 278, 731
BstYI RGATCY 2 cut(s) 673, 841
BtgI CCRYGG 2 cut(s) 13, 465
BtsCI GGATG 2 cut(s) 856, 1185
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 4 cut(s) 171, 320, 537, 1077
CaiI CAGNNNCTG 1 cut(s) 234
CfoI GCGC 1 cut(s) 92
Cfr13I GGNCC 2 cut(s) 80, 136
Cfr9I CCCGGG 1 cut(s) 60
Csp6I GTAC 3 cut(s) 169, 309, 462
CviAII CATG 5 cut(s) 14, 296, 855, 899, 1158
CviQI GTAC 3 cut(s) 169, 309, 462
DdeI CTNAG 2 cut(s) 93, 1087
DpnI GATC 5 cut(s) 57, 363, 654, 675, 843
DpnII GATC 5 cut(s) 55, 361, 652, 673, 841
Eam1104I CTCTTC 1 cut(s) 696
EarI CTCTTC 1 cut(s) 696
Eco130I CCWWGG 2 cut(s) 13, 724
Eco47I GGWCC 2 cut(s) 80, 136
Eco47III AGCGCT 1 cut(s) 91
Eco88I CYCGRG 2 cut(s) 60, 846
Eco91I GGTNACC 2 cut(s) 257, 715
EcoO65I GGTNACC 2 cut(s) 257, 715
EcoRI GAATTC 2 cut(s) 65, 1027
EcoRII CCWGG 1 cut(s) 635
EcoT14I CCWWGG 2 cut(s) 13, 724
EcoT22I ATGCAT 1 cut(s) 856
ErhI CCWWGG 2 cut(s) 13, 724
FaeI CATG 5 cut(s) 17, 299, 858, 902, 1161
FaqI GGGAC 1 cut(s) 122
FatI CATG 5 cut(s) 13, 295, 854, 898, 1157
FauI CCCGC 1 cut(s) 1176
FbaI TGATCA 1 cut(s) 652
Fnu4HI GCNGC 4 cut(s) 104, 828, 861, 1005
FokI GGATG 2 cut(s) 863, 1192
Fsp4HI GCNGC 4 cut(s) 104, 828, 861, 1005
GlaI GCGC 1 cut(s) 91
GluI GCNGC 4 cut(s) 104, 828, 861, 1005
GsaI CCCAGC 2 cut(s) 250, 1136
GsuI CTGGAG 2 cut(s) 651, 891
HaeII RGCGCY 1 cut(s) 93
HapII CCGG 3 cut(s) 61, 78, 209
HhaI GCGC 1 cut(s) 92
Hin1II CATG 5 cut(s) 17, 299, 858, 902, 1161
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HinfI GANTC 7 cut(s) 74, 203, 521, 748, 875, 1082, 1123
HpaII CCGG 3 cut(s) 61, 78, 209
HphI GGTGA 4 cut(s) 530, 553, 707, 727
Hpy166II GTNNAC 2 cut(s) 171, 937
Hpy188I TCNGA 7 cut(s) 49, 71, 96, 687, 841, 1057, 1090
Hpy188III TCNNGA 6 cut(s) 78, 200, 224, 525, 630, 848
Hpy8I GTNNAC 2 cut(s) 171, 937
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 3 cut(s) 156, 656, 704
HpyCH4III ACNGT 5 cut(s) 175, 230, 478, 1098, 1237
HpyCH4V TGCA 6 cut(s) 430, 776, 854, 1007, 1196, 1229
HpyF10VI GCNNNNNNNGC 6 cut(s) 15, 24, 536, 860, 866, 968
HpyF3I CTNAG 2 cut(s) 93, 1087
Hsp92II CATG 5 cut(s) 17, 299, 858, 902, 1161
HspAI GCGC 1 cut(s) 90
Kpn2I TCCGGA 1 cut(s) 77
KpnI GGTACC 2 cut(s) 312, 465
Ksp22I TGATCA 1 cut(s) 652
Kzo9I GATC 5 cut(s) 55, 361, 652, 673, 841
LmnI GCTCC 2 cut(s) 632, 990
Lsp1109I GCAGC 4 cut(s) 115, 839, 847, 991
LweI GCATC 2 cut(s) 841, 1170
MaeIII GTNAC 4 cut(s) 191, 257, 581, 715
MalI GATC 5 cut(s) 57, 363, 654, 675, 843
MboI GATC 5 cut(s) 55, 361, 652, 673, 841
MboII GAAGA 1 cut(s) 713
MflI RGATCY 2 cut(s) 673, 841
MhlI GDGCHC 1 cut(s) 995
MluCI AATT 7 cut(s) 65, 160, 397, 924, 955, 1027, 1197
MlyI GAGTC 3 cut(s) 68, 197, 742
MmeI TCCRAC 2 cut(s) 72, 873
Mph1103I ATGCAT 1 cut(s) 856
MroI TCCGGA 1 cut(s) 77
MroXI GAANNNNTTC 1 cut(s) 1081
MseI TTAA 6 cut(s) 240, 330, 410, 669, 819, 1044
MslI CAYNNNNRTG 1 cut(s) 435
MspA1I CMGCKG 2 cut(s) 962, 1050
MspCI CTTAAG 1 cut(s) 668
MspI CCGG 3 cut(s) 61, 78, 209
MspR9I CCNGG 3 cut(s) 61, 62, 637
Mva1269I GAATGC 1 cut(s) 681
MvaI CCWGG 1 cut(s) 637
MwoI GCNNNNNNNGC 6 cut(s) 15, 24, 536, 860, 866, 968
NciI CCSGG 2 cut(s) 61, 62
NcoI CCATGG 1 cut(s) 13
NdeII GATC 5 cut(s) 55, 361, 652, 673, 841
NlaIII CATG 5 cut(s) 17, 299, 858, 902, 1161
NlaIV GGNNCC 6 cut(s) 82, 138, 310, 463, 634, 675
NmuCI GTSAC 2 cut(s) 581, 715
NsiI ATGCAT 1 cut(s) 856
NspI RCATGY 2 cut(s) 858, 1161
PciI ACATGT 1 cut(s) 1157
PctI GAATGC 1 cut(s) 681
PdmI GAANNNNTTC 1 cut(s) 1081
PfeI GAWTC 4 cut(s) 521, 875, 1082, 1123
PkrI GCNGC 4 cut(s) 105, 829, 862, 1006
PleI GAGTC 3 cut(s) 68, 197, 742
PpsI GAGTC 3 cut(s) 68, 197, 742
PscI ACATGT 1 cut(s) 1157
PsiI TTATAA 1 cut(s) 396
Psp6I CCWGG 1 cut(s) 635
PspEI GGTNACC 2 cut(s) 257, 715
PspFI CCCAGC 2 cut(s) 246, 1132
PspGI CCWGG 1 cut(s) 635
PspN4I GGNNCC 6 cut(s) 82, 138, 310, 463, 634, 675
PspPI GGNCC 2 cut(s) 80, 136
PstNI CAGNNNCTG 1 cut(s) 234
PsuI RGATCY 2 cut(s) 673, 841
PvuII CAGCTG 1 cut(s) 962
RsaI GTAC 3 cut(s) 170, 310, 463
RsaNI GTAC 3 cut(s) 169, 309, 462
RseI CAYNNNNRTG 1 cut(s) 435
SaqAI TTAA 6 cut(s) 240, 330, 410, 669, 819, 1044
SatI GCNGC 4 cut(s) 104, 828, 861, 1005
Sau3AI GATC 5 cut(s) 55, 361, 652, 673, 841
Sau96I GGNCC 2 cut(s) 80, 136
SchI GAGTC 3 cut(s) 68, 197, 742
ScrFI CCNGG 3 cut(s) 61, 62, 637
SduI GDGCHC 1 cut(s) 995
SfaNI GCATC 2 cut(s) 841, 1170
SfcI CTRYAG 2 cut(s) 85, 972
SinI GGWCC 2 cut(s) 80, 136
SmaI CCCGGG 1 cut(s) 62
SmiMI CAYNNNNRTG 1 cut(s) 435
SmlI CTYRAG 2 cut(s) 668, 802
SmoI CTYRAG 2 cut(s) 668, 802
Sse9I AATT 7 cut(s) 65, 160, 397, 924, 955, 1027, 1197
SsiI CCGC 5 cut(s) 27, 539, 566, 1050, 1183
SspI AATATT 1 cut(s) 836
StyD4I CCNGG 3 cut(s) 59, 60, 635
StyI CCWWGG 2 cut(s) 13, 724
TaaI ACNGT 5 cut(s) 175, 230, 478, 1098, 1237
TaqI TCGA 4 cut(s) 524, 792, 1118, 1126
TasI AATT 7 cut(s) 65, 160, 397, 924, 955, 1027, 1197
TatI WGTACW 1 cut(s) 168
TfiI GAWTC 4 cut(s) 521, 875, 1082, 1123
Tru1I TTAA 6 cut(s) 240, 330, 410, 669, 819, 1044
Tru9I TTAA 6 cut(s) 240, 330, 410, 669, 819, 1044
TscAI CASTG 4 cut(s) 178, 320, 537, 1077
TseFI GTSAC 2 cut(s) 581, 715
TseI GCWGC 4 cut(s) 103, 827, 860, 1004
Tsp45I GTSAC 2 cut(s) 581, 715
TspDTI ATGAA 3 cut(s) 714, 915, 1136
TspGWI ACGGA 1 cut(s) 1055
TspMI CCCGGG 1 cut(s) 60
TspRI CASTG 4 cut(s) 178, 320, 537, 1077
Vha464I CTTAAG 1 cut(s) 668
VpaK11BI GGWCC 2 cut(s) 80, 136
XapI RAATTY 2 cut(s) 65, 1027
XceI RCATGY 2 cut(s) 858, 1161
XmaI CCCGGG 1 cut(s) 60
XmnI GAANNNNTTC 1 cut(s) 1081
Zsp2I ATGCAT 1 cut(s) 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.