Rh4AG221100

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0007949
Physical Location & Seq
Forward (+)
52027129 .. 52031544
4416 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG221100.1

Sequence Viewer

Length: 954 bp
ATGGGTTGTGTCCATGGCAAGTGTTGCGGTCGTTTTCCCAAGTCCTCCGACGGCGATCCCCGGGAATTCAGAGACTCCGGACCCTACAGCGCTCAGAGGAAGCAGCACATACTCACAGAGAGGTCGTTGGAGTTGGTCCCCGTTCCTTCACACGGCTTCAATTTGGAGTACACTGTCCTAACGCAGAGGGGTTACTATCCCGACTCGCCGGATAAGGATAATCAGGACAGTTTCTGTGTTAAAACACAGCTTCAAGGTAACCCAAATATCCACTTCTTTGGTGTGTTTGATGGGCATGGTCAATTTGGTACCCAGTGTGCTAATCTTGTTAAGGATAGACTGGTAGAGGTATTAGCAGATGATCCCACATTGCCTGATGACCCTGTGGAAGCTTATAATTCTGCTTTTTTAACCACCAATAGCGAGCTGCATAGTAGTGTAATTGATGACACTATGAGTGGTACCACGGCGATTACTGTGCTTGTGGTTGGGAATACGCTGTATGTTGCGAATGTGGGTGATTCGAGAGCAGTGATTGCGGTGAAAGATGGTAATAGGATTATTGCAGAGGATTTGTCTTGTGACCAAACGCCGTTTAGGAAAGATGAGTATGAGAGAGTGAAGCAGTCTGGAGCCAGGGTTTTGAGTGTTGATCAAGTGGAAGGGCTTAAGGATCCAAGCATTCAGAGTTGGGGTGATGAAGAGAGTGAAGGGGGTGATCCTCCAAGGCTGTGGGTTCAAAATGGTATGTATCCTGGGACTGCATTTACAAGGAGCGTTGGTGATAGTACTGCTGAGACGATTGGTGTTGTTGCAACTCCAGAAGTGTCAATGGTTCAGCTGACTCCCAATCATCTTTTCTTTGTCATTGCAAGTGATGGGGTTTTCGAGTTCCTCTCAAGCCAAGCTGTTGTTAATATGGTACGTAACTTGACCCATGACATACTGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

317

Amino Acids

34.71

Weight (kDa)

4.69

Isoelectric Point (pI)

35.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 78 - 313 2.1e-38 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 396
Acc65I GGTACC 2 cut(s) 308, 461
AccB1I GGYRCC 2 cut(s) 308, 461
AccIII TCCGGA 1 cut(s) 77
AciI CCGC 2 cut(s) 27, 539
AclWI GGATC 5 cut(s) 50, 356, 668, 681, 713
AcsI RAATTY 1 cut(s) 65
AfaI GTAC 5 cut(s) 170, 310, 463, 790, 924
AfeI AGCGCT 1 cut(s) 91
AfiI CCNNNNNNNGG 1 cut(s) 152
AflII CTTAAG 1 cut(s) 668
AgsI TTSAA 3 cut(s) 160, 254, 740
AjnI CCWGG 2 cut(s) 635, 754
AjuI GAANNNNNNNTTGG 2 cut(s) 842, 874
AluBI AGCT 5 cut(s) 250, 392, 427, 841, 908
AluI AGCT 5 cut(s) 250, 392, 427, 841, 908
Alw26I GTCTC 2 cut(s) 66, 791
AlwI GGATC 5 cut(s) 50, 356, 668, 681, 713
AlwNI CAGNNNCTG 1 cut(s) 234
Ama87I CYCGRG 1 cut(s) 60
Aor13HI TCCGGA 1 cut(s) 77
Aor51HI AGCGCT 1 cut(s) 91
ApeKI GCWGC 2 cut(s) 103, 427
ApoI RAATTY 1 cut(s) 65
Asp718I GGTACC 2 cut(s) 308, 461
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 2 cut(s) 80, 136
AsuC2I CCSGG 2 cut(s) 61, 62
AsuHPI GGTGA 5 cut(s) 530, 553, 707, 728, 794
AvaI CYCGRG 1 cut(s) 60
AvaII GGWCC 2 cut(s) 80, 136
BamHI GGATCC 1 cut(s) 673
BanI GGYRCC 2 cut(s) 308, 461
BbvI GCAGC 2 cut(s) 115, 414
BccI CCATC 3 cut(s) 284, 542, 872
BceAI ACGGC 4 cut(s) 67, 169, 483, 577
BcgI CGANNNNNNTGC 2 cut(s) 460, 494
BciT130I CCWGG 2 cut(s) 637, 756
BciVI GTATCC 1 cut(s) 762
BclI TGATCA 1 cut(s) 652
BcnI CCSGG 2 cut(s) 61, 62
BcoDI GTCTC 2 cut(s) 66, 791
BfmI CTRYAG 1 cut(s) 85
BfoI RGCGCY 1 cut(s) 93
BfrI CTTAAG 1 cut(s) 668
BfuI GTATCC 1 cut(s) 762
BisI GCNGC 2 cut(s) 104, 428
BlsI GCNGC 2 cut(s) 105, 429
BmcAI AGTACT 1 cut(s) 790
Bme1390I CCNGG 4 cut(s) 61, 62, 637, 756
Bme18I GGWCC 2 cut(s) 80, 136
BmeT110I CYCGRG 1 cut(s) 60
BmgT120I GGNCC 2 cut(s) 80, 136
BmiI GGNNCC 6 cut(s) 82, 138, 310, 463, 634, 675
BmrFI CCNGG 4 cut(s) 61, 62, 637, 756
BmrI ACTGGG 1 cut(s) 307
BmuI ACTGGG 1 cut(s) 307
BplI GAGNNNNNCTC 2 cut(s) 881, 913
BpmI CTGGAG 2 cut(s) 651, 804
BpuEI CTTGAG 1 cut(s) 883
BpuMI CCSGG 2 cut(s) 61, 62
BsaAI YACGTR 1 cut(s) 926
BsaJI CCNNGG 7 cut(s) 13, 59, 60, 465, 636, 725, 755
BsaWI WCCGGW 1 cut(s) 77
BsaXI ACNNNNNCTCC 4 cut(s) 624, 654, 766, 796
Bsc4I CCNNNNNNNGG 1 cut(s) 152
Bse1I ACTGG 2 cut(s) 313, 345
Bse3DI GCAATG 2 cut(s) 368, 867
BseAI TCCGGA 1 cut(s) 77
BseBI CCWGG 2 cut(s) 637, 756
BseDI CCNNGG 7 cut(s) 13, 59, 60, 465, 636, 725, 755
BseLI CCNNNNNNNGG 1 cut(s) 152
BseMI GCAATG 2 cut(s) 368, 867
BseMII CTCAG 2 cut(s) 107, 786
BseNI ACTGG 2 cut(s) 313, 345
BseXI GCAGC 2 cut(s) 115, 414
Bsh1285I CGRYCG 1 cut(s) 31
BshNI GGYRCC 2 cut(s) 308, 461
BsiEI CGRYCG 1 cut(s) 31
BsiHKCI CYCGRG 1 cut(s) 60
BsiSI CCGG 3 cut(s) 61, 78, 209
BslFI GGGAC 2 cut(s) 122, 772
BslI CCNNNNNNNGG 1 cut(s) 152
BsmAI GTCTC 2 cut(s) 66, 791
BsmBI CGTCTC 1 cut(s) 791
BsmFI GGGAC 2 cut(s) 122, 772
BsmI GAATGC 1 cut(s) 681
BsoBI CYCGRG 1 cut(s) 60
Bsp13I TCCGGA 1 cut(s) 77
Bsp143I GATC 5 cut(s) 55, 361, 652, 673, 718
Bsp19I CCATGG 1 cut(s) 13
BspACI CCGC 2 cut(s) 27, 539
BspCNI CTCAG 2 cut(s) 106, 787
BspEI TCCGGA 1 cut(s) 77
BspLI GGNNCC 6 cut(s) 82, 138, 310, 463, 634, 675
BspPI GGATC 5 cut(s) 50, 356, 668, 681, 713
BspT107I GGYRCC 2 cut(s) 308, 461
BspTI CTTAAG 1 cut(s) 668
BsrDI GCAATG 2 cut(s) 368, 867
BsrI ACTGG 2 cut(s) 313, 345
BssECI CCNNGG 7 cut(s) 13, 59, 60, 465, 636, 725, 755
BssMI GATC 5 cut(s) 55, 361, 652, 673, 718
BssT1I CCWWGG 2 cut(s) 13, 725
Bst2UI CCWGG 2 cut(s) 637, 756
Bst4CI ACNGT 3 cut(s) 175, 230, 478
Bst6I CTCTTC 1 cut(s) 696
BstAFI CTTAAG 1 cut(s) 668
BstAPI GCANNNNNTGC 2 cut(s) 24, 536
BstBAI YACGTR 1 cut(s) 926
BstC8I GCNNGC 1 cut(s) 425
BstDEI CTNAG 2 cut(s) 93, 795
BstDSI CCRYGG 2 cut(s) 13, 465
BstEII GGTNACC 1 cut(s) 257
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 5 cut(s) 58, 364, 655, 676, 721
BstMAI GTCTC 2 cut(s) 66, 791
BstMBI GATC 5 cut(s) 55, 361, 652, 673, 718
BstMCI CGRYCG 1 cut(s) 31
BstMWI GCNNNNNNNGC 2 cut(s) 24, 536
BstNI CCWGG 2 cut(s) 637, 756
BstPI GGTNACC 1 cut(s) 257
BstSCI CCNGG 4 cut(s) 59, 60, 635, 754
BstSFI CTRYAG 1 cut(s) 85
BstSNI TACGTA 1 cut(s) 926
BstV1I GCAGC 2 cut(s) 115, 414
BstX2I RGATCY 1 cut(s) 673
BstXI CCANNNNNNTGG 2 cut(s) 278, 732
BstYI RGATCY 1 cut(s) 673
BsuI GTATCC 1 cut(s) 762
BtgI CCRYGG 2 cut(s) 13, 465
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 3 cut(s) 171, 320, 537
Cac8I GCNNGC 1 cut(s) 425
CaiI CAGNNNCTG 1 cut(s) 234
CfoI GCGC 1 cut(s) 92
Cfr13I GGNCC 2 cut(s) 80, 136
Cfr9I CCCGGG 1 cut(s) 60
Csp6I GTAC 5 cut(s) 169, 309, 462, 789, 923
CviAII CATG 3 cut(s) 14, 296, 938
CviQI GTAC 5 cut(s) 169, 309, 462, 789, 923
DdeI CTNAG 2 cut(s) 93, 795
DpnI GATC 5 cut(s) 57, 363, 654, 675, 720
DpnII GATC 5 cut(s) 55, 361, 652, 673, 718
Eam1104I CTCTTC 1 cut(s) 696
EarI CTCTTC 1 cut(s) 696
Eco105I TACGTA 1 cut(s) 926
Eco130I CCWWGG 2 cut(s) 13, 725
Eco47I GGWCC 2 cut(s) 80, 136
Eco47III AGCGCT 1 cut(s) 91
Eco88I CYCGRG 1 cut(s) 60
Eco91I GGTNACC 1 cut(s) 257
EcoO65I GGTNACC 1 cut(s) 257
EcoRI GAATTC 1 cut(s) 65
EcoRII CCWGG 2 cut(s) 635, 754
EcoT14I CCWWGG 2 cut(s) 13, 725
ErhI CCWWGG 2 cut(s) 13, 725
Esp3I CGTCTC 1 cut(s) 791
FaeI CATG 3 cut(s) 17, 299, 941
FaqI GGGAC 2 cut(s) 122, 772
FatI CATG 3 cut(s) 13, 295, 937
FbaI TGATCA 1 cut(s) 652
Fnu4HI GCNGC 2 cut(s) 104, 428
Fsp4HI GCNGC 2 cut(s) 104, 428
GlaI GCGC 1 cut(s) 91
GluI GCNGC 2 cut(s) 104, 428
GsuI CTGGAG 2 cut(s) 651, 804
HaeII RGCGCY 1 cut(s) 93
HapII CCGG 3 cut(s) 61, 78, 209
HhaI GCGC 1 cut(s) 92
Hin1II CATG 3 cut(s) 17, 299, 941
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HindIII AAGCTT 1 cut(s) 390
HinfI GANTC 4 cut(s) 74, 203, 521, 844
HpaII CCGG 3 cut(s) 61, 78, 209
HphI GGTGA 5 cut(s) 530, 553, 707, 728, 794
Hpy166II GTNNAC 1 cut(s) 171
Hpy188I TCNGA 4 cut(s) 49, 71, 96, 687
Hpy188III TCNNGA 6 cut(s) 78, 200, 224, 525, 630, 821
Hpy8I GTNNAC 1 cut(s) 171
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 3 cut(s) 156, 656, 704
HpyCH4III ACNGT 3 cut(s) 175, 230, 478
HpyCH4IV ACGT 1 cut(s) 925
HpyCH4V TGCA 5 cut(s) 430, 566, 764, 815, 872
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 536
HpyF3I CTNAG 2 cut(s) 93, 795
HpySE526I ACGT 1 cut(s) 925
Hsp92II CATG 3 cut(s) 17, 299, 941
HspAI GCGC 1 cut(s) 90
Kpn2I TCCGGA 1 cut(s) 77
KpnI GGTACC 2 cut(s) 312, 465
Ksp22I TGATCA 1 cut(s) 652
Kzo9I GATC 5 cut(s) 55, 361, 652, 673, 718
LmnI GCTCC 2 cut(s) 632, 774
Lsp1109I GCAGC 2 cut(s) 115, 414
MaeII ACGT 1 cut(s) 925
MaeIII GTNAC 4 cut(s) 191, 257, 581, 926
MalI GATC 5 cut(s) 57, 363, 654, 675, 720
MboI GATC 5 cut(s) 55, 361, 652, 673, 718
MboII GAAGA 1 cut(s) 713
MflI RGATCY 1 cut(s) 673
MluCI AATT 5 cut(s) 65, 160, 302, 397, 441
MlyI GAGTC 3 cut(s) 68, 197, 838
MmeI TCCRAC 2 cut(s) 72, 108
MnlI CCTC 8 cut(s) 55, 90, 114, 180, 340, 562, 732, 905
MroI TCCGGA 1 cut(s) 77
MseI TTAA 6 cut(s) 240, 330, 410, 669, 915, 952
MslI CAYNNNNRTG 1 cut(s) 435
MspA1I CMGCKG 1 cut(s) 841
MspCI CTTAAG 1 cut(s) 668
MspI CCGG 3 cut(s) 61, 78, 209
MspR9I CCNGG 4 cut(s) 61, 62, 637, 756
Mva1269I GAATGC 1 cut(s) 681
MvaI CCWGG 2 cut(s) 637, 756
MwoI GCNNNNNNNGC 2 cut(s) 24, 536
NciI CCSGG 2 cut(s) 61, 62
NcoI CCATGG 1 cut(s) 13
NdeII GATC 5 cut(s) 55, 361, 652, 673, 718
NlaIII CATG 3 cut(s) 17, 299, 941
NlaIV GGNNCC 6 cut(s) 82, 138, 310, 463, 634, 675
NmuCI GTSAC 1 cut(s) 581
PctI GAATGC 1 cut(s) 681
PfeI GAWTC 1 cut(s) 521
PkrI GCNGC 2 cut(s) 105, 429
PleI GAGTC 3 cut(s) 68, 197, 838
PpsI GAGTC 3 cut(s) 68, 197, 838
Ppu21I YACGTR 1 cut(s) 926
PsiI TTATAA 1 cut(s) 396
Psp6I CCWGG 2 cut(s) 635, 754
PspEI GGTNACC 1 cut(s) 257
PspGI CCWGG 2 cut(s) 635, 754
PspN4I GGNNCC 6 cut(s) 82, 138, 310, 463, 634, 675
PspPI GGNCC 2 cut(s) 80, 136
PstNI CAGNNNCTG 1 cut(s) 234
PsuI RGATCY 1 cut(s) 673
PvuII CAGCTG 1 cut(s) 841
RsaI GTAC 5 cut(s) 170, 310, 463, 790, 924
RsaNI GTAC 5 cut(s) 169, 309, 462, 789, 923
RseI CAYNNNNRTG 1 cut(s) 435
SaqAI TTAA 6 cut(s) 240, 330, 410, 669, 915, 952
SatI GCNGC 2 cut(s) 104, 428
Sau3AI GATC 5 cut(s) 55, 361, 652, 673, 718
Sau96I GGNCC 2 cut(s) 80, 136
ScaI AGTACT 1 cut(s) 790
SchI GAGTC 3 cut(s) 68, 197, 838
ScrFI CCNGG 4 cut(s) 61, 62, 637, 756
SetI ASST 9 cut(s) 125, 252, 259, 351, 394, 429, 843, 910, 928
SfcI CTRYAG 1 cut(s) 85
SinI GGWCC 2 cut(s) 80, 136
SmaI CCCGGG 1 cut(s) 62
SmiMI CAYNNNNRTG 1 cut(s) 435
SmlI CTYRAG 2 cut(s) 668, 898
SmoI CTYRAG 2 cut(s) 668, 898
SnaBI TACGTA 1 cut(s) 926
Sse9I AATT 5 cut(s) 65, 160, 302, 397, 441
SsiI CCGC 2 cut(s) 27, 539
StyD4I CCNGG 4 cut(s) 59, 60, 635, 754
StyI CCWWGG 2 cut(s) 13, 725
TaaI ACNGT 3 cut(s) 175, 230, 478
TaiI ACGT 1 cut(s) 928
TaqI TCGA 2 cut(s) 524, 888
TasI AATT 5 cut(s) 65, 160, 302, 397, 441
TatI WGTACW 2 cut(s) 168, 788
TfiI GAWTC 1 cut(s) 521
Tru1I TTAA 6 cut(s) 240, 330, 410, 669, 915, 952
Tru9I TTAA 6 cut(s) 240, 330, 410, 669, 915, 952
TscAI CASTG 3 cut(s) 178, 320, 537
TseFI GTSAC 1 cut(s) 581
TseI GCWGC 2 cut(s) 103, 427
Tsp45I GTSAC 1 cut(s) 581
TspDTI ATGAA 1 cut(s) 714
TspMI CCCGGG 1 cut(s) 60
TspRI CASTG 3 cut(s) 178, 320, 537
Vha464I CTTAAG 1 cut(s) 668
VpaK11BI GGWCC 2 cut(s) 80, 136
XapI RAATTY 1 cut(s) 65
XmaI CCCGGG 1 cut(s) 60
ZrmI AGTACT 1 cut(s) 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.