Prupe.1G147500_v2.0.a1

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0007949
Physical Location & Seq
Reverse (-)
11579749 .. 11584785
5037 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G147500.1

Sequence Viewer

Length: 1275 bp
ATGGGTTGTGTCCATGGCAAGTGTTGCGGTCGTTACCCCCAGTCTTCCGATGGTGATCTCCGGGACTTCAGAGACGCTAGTCCGTACAGTGCGCAAAGGAAGCACATACTCTCAGGGAGGTCATTGGAGCTCGTCCCTGTTCCTTCACATGACTTCCGTTTGGAGTACTCTCTGCTAACGCAGCGTGGTTACTATCCCGACTCGCCGGATAAGGAAAACCAGGACAGTTTCTGTGTTAGAACGCAAGTCAGAGGTAACCCAAATATCCATTTCTTTGGTGTGTTTGATGGGCATGGCCAATTTGGTACCCAGTGTTCTAATTTTGTTAAGGACAGGCTAGTAGAGATATTAGCAAATGATCCGACATTGTCAGCTGACCCTGTGAAAGCTTATAATTCTGCCTTTTTGACGACGAATTATGAGTTGCATAATAGTGACATTGATGATACTATGAGTGGAACCACGGCAATTACTGTGCTTGTTATTGGGAATACCCTTTATGTTGCGAATGTGGGTGATTCGAGAGCTGTGGTTGCAGTGAAAGATGGTAATAGAATTATTGCAGAGGACTTGTCTTATGACCAAACACCATTTAGGAAAGACGAGTATGGGAGAGTGAAGCTTTGTGGGGCCAGGGTTTTGAGTGTTGATCAAGTGGAAGGGCTGAAGGATCCAGATATCCAGACCTGGGGTGATGAAGAGAGTGAAGGCGGTGATCCTCCCAGGCTGTGGGTTCAAAATGGGATGTATCCTGGGACTGCGTTTACAAGGAGTGTAGGTGATAGTACAGCTGAGAAGATTGGTGTTGTTGCAACTCCAGAGGTGTCAATGCTTCAGCTGACACCTAATCATCTCTTCTTTGTTGTCGCAAGTGATGGGGTTTTTGAGTTCCTCTCTAGCCAAGCCGTGGTTAATATGGCTTCAAAATATTCAGATCCCCGGGATGCATGTGCTGCCATTGCTGGAGAATCATACAAATTGTGGTTGGAACATGAAAACCGGACAGATGATATAACAATTATCATTGTTCACATCAAAGGCTTGTCTAATTCAGGTGGTGGTGTTACTGATGGAACAAGTGGGACCAATAATAGACCAGCAACAAGGATGAGAAAAGTTTCTGAATCATCTGTTACCACTGGGTCAGAAGTTTTCCGTTCAGTGAGAAGTGATTCCTCAGAAGTACAGTCTTGTCAGCTTGTGCTTTCGATGAATCGGAGCCCAGCTATTGTTGTTCCGTCGCCTGCATGTCAGAGCCCTTGGAGTTGTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

425

Amino Acids

46.29

Weight (kDa)

5.07

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 393
Acc16I TGCGCA 1 cut(s) 93
Acc65I GGTACC 1 cut(s) 305
AccB1I GGYRCC 1 cut(s) 305
AccB7I CCANNNNNTGG 2 cut(s) 729, 907
AciI CCGC 2 cut(s) 27, 711
AclWI GGATC 5 cut(s) 353, 665, 678, 710, 929
AcoI YGGCCR 1 cut(s) 295
AcuI CTGAAG 3 cut(s) 52, 686, 818
AfaI GTAC 5 cut(s) 86, 167, 307, 787, 1185
AfiI CCNNNNNNNGG 3 cut(s) 688, 729, 907
AgsI TTSAA 2 cut(s) 737, 924
AjnI CCWGG 5 cut(s) 219, 632, 686, 722, 751
AluBI AGCT 9 cut(s) 130, 374, 389, 527, 622, 791, 838, 1198, 1226
AluI AGCT 9 cut(s) 130, 374, 389, 527, 622, 791, 838, 1198, 1226
Alw21I GWGCWC 1 cut(s) 132
Alw26I GTCTC 1 cut(s) 66
AlwI GGATC 5 cut(s) 353, 665, 678, 710, 929
AlwNI CAGNNNCTG 1 cut(s) 231
Ama87I CYCGRG 1 cut(s) 939
AoxI GGCC 2 cut(s) 295, 630
ApeKI GCWGC 2 cut(s) 181, 953
Asp700I GAANNNNTTC 2 cut(s) 1117, 1171
Asp718I GGTACC 1 cut(s) 305
AspLEI GCGC 1 cut(s) 94
AspS9I GGNCC 2 cut(s) 630, 1083
AsuC2I CCSGG 3 cut(s) 62, 940, 941
AsuHPI GGTGA 5 cut(s) 65, 527, 704, 725, 791
AvaI CYCGRG 1 cut(s) 939
AvaII GGWCC 1 cut(s) 1083
BalI TGGCCA 1 cut(s) 297
BamHI GGATCC 1 cut(s) 670
BanI GGYRCC 1 cut(s) 305
BanII GRGCYC 3 cut(s) 132, 1223, 1259
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 2 cut(s) 193, 940
BccI CCATC 5 cut(s) 44, 281, 539, 869, 1064
BceAI ACGGC 2 cut(s) 480, 890
BciT130I CCWGG 5 cut(s) 221, 634, 688, 724, 753
BciVI GTATCC 1 cut(s) 759
BclI TGATCA 1 cut(s) 649
BcnI CCSGG 3 cut(s) 62, 940, 941
BcoDI GTCTC 1 cut(s) 66
BfaI CTAG 3 cut(s) 78, 338, 897
BfuI GTATCC 1 cut(s) 759
BisI GCNGC 2 cut(s) 182, 954
BlsI GCNGC 2 cut(s) 183, 955
BmcAI AGTACT 1 cut(s) 167
Bme1390I CCNGG 8 cut(s) 62, 221, 634, 688, 724, 753, 940, 941
Bme18I GGWCC 1 cut(s) 1083
BmeT110I CYCGRG 1 cut(s) 939
BmgT120I GGNCC 2 cut(s) 630, 1083
BmiI GGNNCC 6 cut(s) 307, 460, 631, 672, 1084, 1220
BmrFI CCNGG 8 cut(s) 62, 221, 634, 688, 724, 753, 940, 941
BmrI ACTGGG 3 cut(s) 34, 304, 1149
BmsI GCATC 1 cut(s) 934
BmuI ACTGGG 3 cut(s) 34, 304, 1149
BoxI GACNNNNGTC 1 cut(s) 78
BpiI GAAGAC 1 cut(s) 36
BplI GAGNNNNNCTC 2 cut(s) 878, 910
BpmI CTGGAG 2 cut(s) 801, 984
BpuMI CCSGG 3 cut(s) 62, 940, 941
BsaBI GATNNNNATC 1 cut(s) 54
BsaWI WCCGGW 1 cut(s) 999
BsaXI ACNNNNNCTCC 2 cut(s) 763, 793
Bsc4I CCNNNNNNNGG 3 cut(s) 688, 729, 907
Bse1I ACTGG 3 cut(s) 40, 310, 1144
Bse3DI GCAATG 1 cut(s) 957
Bse8I GATNNNNATC 1 cut(s) 54
BseBI CCWGG 5 cut(s) 221, 634, 688, 724, 753
BseGI GGATG 3 cut(s) 750, 949, 1113
BseJI GATNNNNATC 1 cut(s) 54
BseLI CCNNNNNNNGG 3 cut(s) 688, 729, 907
BseMI GCAATG 1 cut(s) 957
BseMII CTCAG 3 cut(s) 126, 783, 1191
BseNI ACTGG 3 cut(s) 40, 310, 1144
BseXI GCAGC 2 cut(s) 193, 940
BseYI CCCAGC 1 cut(s) 1222
Bsh1285I CGRYCG 1 cut(s) 31
BshFI GGCC 2 cut(s) 297, 632
BshNI GGYRCC 1 cut(s) 305
BsiEI CGRYCG 1 cut(s) 31
BsiHKAI GWGCWC 1 cut(s) 132
BsiHKCI CYCGRG 1 cut(s) 939
BsiSI CCGG 4 cut(s) 61, 206, 940, 1000
BslFI GGGAC 4 cut(s) 77, 119, 769, 1096
BslI CCNNNNNNNGG 3 cut(s) 688, 729, 907
BsmAI GTCTC 1 cut(s) 66
BsmBI CGTCTC 1 cut(s) 66
BsmFI GGGAC 4 cut(s) 77, 119, 769, 1096
BsnI GGCC 2 cut(s) 297, 632
BsoBI CYCGRG 1 cut(s) 939
Bsp1286I GDGCHC 3 cut(s) 132, 1223, 1259
Bsp143I GATC 6 cut(s) 55, 358, 649, 670, 715, 934
Bsp19I CCATGG 1 cut(s) 13
BspACI CCGC 2 cut(s) 27, 711
BspANI GGCC 2 cut(s) 297, 632
BspCNI CTCAG 3 cut(s) 125, 784, 1190
BspLI GGNNCC 6 cut(s) 307, 460, 631, 672, 1084, 1220
BspPI GGATC 5 cut(s) 353, 665, 678, 710, 929
BspT107I GGYRCC 1 cut(s) 305
BsrDI GCAATG 1 cut(s) 957
BsrI ACTGG 3 cut(s) 40, 310, 1144
BssMI GATC 6 cut(s) 55, 358, 649, 670, 715, 934
BssT1I CCWWGG 2 cut(s) 13, 1259
Bst2UI CCWGG 5 cut(s) 221, 634, 688, 724, 753
Bst4CI ACNGT 4 cut(s) 89, 227, 475, 1188
Bst6I CTCTTC 2 cut(s) 693, 860
BstAPI GCANNNNNTGC 2 cut(s) 24, 953
BstC8I GCNNGC 1 cut(s) 1245
BstDEI CTNAG 3 cut(s) 112, 792, 1177
BstDSI CCRYGG 3 cut(s) 13, 462, 906
BstEII GGTNACC 1 cut(s) 254
BstF5I GGATG 3 cut(s) 750, 949, 1113
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 6 cut(s) 58, 361, 652, 673, 718, 937
BstMAI GTCTC 1 cut(s) 66
BstMBI GATC 6 cut(s) 55, 358, 649, 670, 715, 934
BstMCI CGRYCG 1 cut(s) 31
BstMWI GCNNNNNNNGC 6 cut(s) 24, 100, 181, 533, 953, 959
BstNI CCWGG 5 cut(s) 221, 634, 688, 724, 753
BstNSI RCATGY 2 cut(s) 951, 1251
BstPAI GACNNNNGTC 1 cut(s) 78
BstPI GGTNACC 1 cut(s) 254
BstSCI CCNGG 8 cut(s) 60, 219, 632, 686, 722, 751, 938, 939
BstV1I GCAGC 2 cut(s) 193, 940
BstV2I GAAGAC 1 cut(s) 36
BstX2I RGATCY 2 cut(s) 670, 934
BstXI CCANNNNNNTGG 1 cut(s) 275
BstYI RGATCY 2 cut(s) 670, 934
BsuI GTATCC 1 cut(s) 759
BsuRI GGCC 2 cut(s) 297, 632
BtgI CCRYGG 3 cut(s) 13, 462, 906
BtsCI GGATG 3 cut(s) 750, 949, 1113
BtsI GCAGTG 1 cut(s) 543
BtsIMutI CAGTG 5 cut(s) 94, 317, 543, 1137, 1167
Cac8I GCNNGC 1 cut(s) 1245
CaiI CAGNNNCTG 1 cut(s) 231
CfoI GCGC 1 cut(s) 94
Cfr13I GGNCC 2 cut(s) 630, 1083
Cfr9I CCCGGG 1 cut(s) 939
CseI GACGC 1 cut(s) 83
Csp6I GTAC 5 cut(s) 85, 166, 306, 786, 1184
CviAII CATG 6 cut(s) 14, 149, 293, 948, 992, 1248
CviQI GTAC 5 cut(s) 85, 166, 306, 786, 1184
DdeI CTNAG 3 cut(s) 112, 792, 1177
DpnI GATC 6 cut(s) 57, 360, 651, 672, 717, 936
DpnII GATC 6 cut(s) 55, 358, 649, 670, 715, 934
EaeI YGGCCR 1 cut(s) 295
Eam1104I CTCTTC 2 cut(s) 693, 860
EarI CTCTTC 2 cut(s) 693, 860
Ecl136II GAGCTC 1 cut(s) 130
Eco130I CCWWGG 2 cut(s) 13, 1259
Eco24I GRGCYC 3 cut(s) 132, 1223, 1259
Eco32I GATATC 1 cut(s) 679
Eco47I GGWCC 1 cut(s) 1083
Eco53kI GAGCTC 1 cut(s) 130
Eco57I CTGAAG 3 cut(s) 52, 686, 818
Eco88I CYCGRG 1 cut(s) 939
Eco91I GGTNACC 1 cut(s) 254
EcoICRI GAGCTC 1 cut(s) 130
EcoO65I GGTNACC 1 cut(s) 254
EcoRII CCWGG 5 cut(s) 219, 632, 686, 722, 751
EcoRV GATATC 1 cut(s) 679
EcoT14I CCWWGG 2 cut(s) 13, 1259
EcoT22I ATGCAT 1 cut(s) 949
EcoT38I GRGCYC 3 cut(s) 132, 1223, 1259
ErhI CCWWGG 2 cut(s) 13, 1259
Esp3I CGTCTC 1 cut(s) 66
FaeI CATG 6 cut(s) 17, 152, 296, 951, 995, 1251
FaqI GGGAC 4 cut(s) 77, 119, 769, 1096
FatI CATG 6 cut(s) 13, 148, 292, 947, 991, 1247
FbaI TGATCA 1 cut(s) 649
Fnu4HI GCNGC 2 cut(s) 182, 954
FokI GGATG 3 cut(s) 757, 956, 1120
FriOI GRGCYC 3 cut(s) 132, 1223, 1259
Fsp4HI GCNGC 2 cut(s) 182, 954
FspBI CTAG 3 cut(s) 78, 338, 897
FspI TGCGCA 1 cut(s) 93
GlaI GCGC 1 cut(s) 93
GluI GCNGC 2 cut(s) 182, 954
GsaI CCCAGC 1 cut(s) 1226
GsuI CTGGAG 2 cut(s) 801, 984
HaeIII GGCC 2 cut(s) 297, 632
HapII CCGG 4 cut(s) 61, 206, 940, 1000
HgaI GACGC 1 cut(s) 83
HhaI GCGC 1 cut(s) 94
Hin1II CATG 6 cut(s) 17, 152, 296, 951, 995, 1251
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HindIII AAGCTT 2 cut(s) 387, 620
HinfI GANTC 6 cut(s) 200, 518, 968, 1124, 1172, 1213
HpaII CCGG 4 cut(s) 61, 206, 940, 1000
HphI GGTGA 5 cut(s) 65, 527, 704, 725, 791
Hpy166II GTNNAC 2 cut(s) 765, 1030
Hpy188III TCNNGA 5 cut(s) 197, 522, 674, 682, 818
Hpy8I GTNNAC 2 cut(s) 765, 1030
Hpy99I CGWCG 2 cut(s) 415, 1243
HpyAV CCTTC 4 cut(s) 153, 653, 661, 701
HpyCH4III ACNGT 4 cut(s) 89, 227, 475, 1188
HpyCH4V TGCA 6 cut(s) 427, 536, 563, 812, 947, 1247
HpyF10VI GCNNNNNNNGC 6 cut(s) 24, 100, 181, 533, 953, 959
HpyF3I CTNAG 3 cut(s) 112, 792, 1177
Hsp92II CATG 6 cut(s) 17, 152, 296, 951, 995, 1251
HspAI GCGC 1 cut(s) 92
KpnI GGTACC 1 cut(s) 309
Ksp22I TGATCA 1 cut(s) 649
Kzo9I GATC 6 cut(s) 55, 358, 649, 670, 715, 934
LmnI GCTCC 2 cut(s) 127, 1218
Lsp1109I GCAGC 2 cut(s) 193, 940
LweI GCATC 1 cut(s) 934
MaeI CTAG 3 cut(s) 78, 338, 897
MaeIII GTNAC 6 cut(s) 32, 188, 254, 434, 1063, 1132
MalI GATC 6 cut(s) 57, 360, 651, 672, 717, 936
MboI GATC 6 cut(s) 55, 358, 649, 670, 715, 934
MboII GAAGA 4 cut(s) 36, 710, 808, 847
MflI RGATCY 2 cut(s) 670, 934
MhlI GDGCHC 3 cut(s) 132, 1223, 1259
MlsI TGGCCA 1 cut(s) 297
MluCI AATT 9 cut(s) 299, 319, 394, 415, 468, 555, 977, 1017, 1048
MluNI TGGCCA 1 cut(s) 297
MlyI GAGTC 1 cut(s) 194
MmeI TCCRAC 2 cut(s) 386, 966
MnlI CCTC 7 cut(s) 111, 245, 559, 729, 814, 902, 1186
Mox20I TGGCCA 1 cut(s) 297
Mph1103I ATGCAT 1 cut(s) 949
MroXI GAANNNNTTC 2 cut(s) 1117, 1171
MscI TGGCCA 1 cut(s) 297
MseI TTAA 2 cut(s) 327, 912
MslI CAYNNNNRTG 1 cut(s) 432
Msp20I TGGCCA 1 cut(s) 297
MspA1I CMGCKG 3 cut(s) 374, 791, 838
MspI CCGG 4 cut(s) 61, 206, 940, 1000
MspR9I CCNGG 8 cut(s) 62, 221, 634, 688, 724, 753, 940, 941
MvaI CCWGG 5 cut(s) 221, 634, 688, 724, 753
MwoI GCNNNNNNNGC 6 cut(s) 24, 100, 181, 533, 953, 959
NciI CCSGG 3 cut(s) 62, 940, 941
NcoI CCATGG 1 cut(s) 13
NdeII GATC 6 cut(s) 55, 358, 649, 670, 715, 934
NlaIII CATG 6 cut(s) 17, 152, 296, 951, 995, 1251
NlaIV GGNNCC 6 cut(s) 307, 460, 631, 672, 1084, 1220
NmuCI GTSAC 1 cut(s) 434
NsbI TGCGCA 1 cut(s) 93
NsiI ATGCAT 1 cut(s) 949
NspI RCATGY 2 cut(s) 951, 1251
PdmI GAANNNNTTC 2 cut(s) 1117, 1171
PfeI GAWTC 5 cut(s) 518, 968, 1124, 1172, 1213
PflFI GACNNNGTC 1 cut(s) 367
PflMI CCANNNNNTGG 2 cut(s) 729, 907
PfoI TCCNGGA 1 cut(s) 60
PkrI GCNGC 2 cut(s) 183, 955
PleI GAGTC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 194
PshAI GACNNNNGTC 1 cut(s) 78
PsiI TTATAA 1 cut(s) 393
Psp124BI GAGCTC 1 cut(s) 132
Psp6I CCWGG 5 cut(s) 219, 632, 686, 722, 751
PspEI GGTNACC 1 cut(s) 254
PspFI CCCAGC 1 cut(s) 1222
PspGI CCWGG 5 cut(s) 219, 632, 686, 722, 751
PspN4I GGNNCC 6 cut(s) 307, 460, 631, 672, 1084, 1220
PspPI GGNCC 2 cut(s) 630, 1083
PsrI GAACNNNNNNTAC 2 cut(s) 298, 330
PstNI CAGNNNCTG 1 cut(s) 231
PsuI RGATCY 2 cut(s) 670, 934
PsyI GACNNNGTC 1 cut(s) 367
PvuII CAGCTG 3 cut(s) 374, 791, 838
RsaI GTAC 5 cut(s) 86, 167, 307, 787, 1185
RsaNI GTAC 5 cut(s) 85, 166, 306, 786, 1184
RseI CAYNNNNRTG 1 cut(s) 432
SacI GAGCTC 1 cut(s) 132
SaqAI TTAA 2 cut(s) 327, 912
SatI GCNGC 2 cut(s) 182, 954
Sau3AI GATC 6 cut(s) 55, 358, 649, 670, 715, 934
Sau96I GGNCC 2 cut(s) 630, 1083
ScaI AGTACT 1 cut(s) 167
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 8 cut(s) 62, 221, 634, 688, 724, 753, 940, 941
SduI GDGCHC 3 cut(s) 132, 1223, 1259
SfaNI GCATC 1 cut(s) 934
SinI GGWCC 1 cut(s) 1083
SmaI CCCGGG 1 cut(s) 941
SmiMI CAYNNNNRTG 1 cut(s) 432
Sse9I AATT 9 cut(s) 299, 319, 394, 415, 468, 555, 977, 1017, 1048
SsiI CCGC 2 cut(s) 27, 711
SspI AATATT 1 cut(s) 929
SspMI CTAG 3 cut(s) 78, 338, 897
SstI GAGCTC 1 cut(s) 132
StyD4I CCNGG 8 cut(s) 60, 219, 632, 686, 722, 751, 938, 939
StyI CCWWGG 2 cut(s) 13, 1259
TaaI ACNGT 4 cut(s) 89, 227, 475, 1188
TaqI TCGA 2 cut(s) 521, 1208
TasI AATT 9 cut(s) 299, 319, 394, 415, 468, 555, 977, 1017, 1048
TatI WGTACW 3 cut(s) 165, 785, 1183
TfiI GAWTC 5 cut(s) 518, 968, 1124, 1172, 1213
Tru1I TTAA 2 cut(s) 327, 912
Tru9I TTAA 2 cut(s) 327, 912
TscAI CASTG 5 cut(s) 94, 317, 543, 1144, 1167
TseFI GTSAC 1 cut(s) 434
TseI GCWGC 2 cut(s) 181, 953
Tsp45I GTSAC 1 cut(s) 434
TspDTI ATGAA 3 cut(s) 711, 1008, 1226
TspGWI ACGGA 4 cut(s) 72, 146, 1145, 1227
TspMI CCCGGG 1 cut(s) 939
TspRI CASTG 5 cut(s) 94, 317, 543, 1144, 1167
Tth111I GACNNNGTC 1 cut(s) 367
Van91I CCANNNNNTGG 2 cut(s) 729, 907
VpaK11BI GGWCC 1 cut(s) 1083
XceI RCATGY 2 cut(s) 951, 1251
XcmI CCANNNNNNNNNTGG 1 cut(s) 47
XmaI CCCGGG 1 cut(s) 939
XmnI GAANNNNTTC 2 cut(s) 1117, 1171
XspI CTAG 3 cut(s) 78, 338, 897
ZrmI AGTACT 1 cut(s) 167
Zsp2I ATGCAT 1 cut(s) 949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.