Rh4DG220100

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0007949
Physical Location & Seq
Forward (+)
42166390 .. 42169258
2869 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG220100.1

Sequence Viewer

Length: 1281 bp
ATGGGTTGTGTCCATGGCAAGTGTTGCGGTCGTTTTCCCAAGTCCTCCGACGGCGATCCCCGGGAATTCAGAGACTCCGGACCCTACAGCGCTCAGAGGAAGCAGCACATACTCACAGAGAGGTCATTGGAGTTGGTCCCCGTTCCTTCACACGGCTTCAATTTGGAGTACACTGTCCTAACGCAGAGGGGTTACTATCCCGACTCGCCGGATAAGGATAATCAGGACAGTTTCTGTGTTAAAACACAGCTTCAAGGTAACCCAAATATCCATTTCTTTGGTGTGTTTGATGGGCATGGTCAATTTGGTACCCAGTGTGCTAATCTTGTTAAGGATAGACTGGTAGAGGTATTAGCAGATGATCCCACATTGCCTGATGACCCTGTGGAAGCTTATAATTCTGCTTTTTTAACCACGAATAGCGAGCTGCATAGTAGTGTAATTGATGATACTATGAGTGGAACCACGGCGATTACTGTGCTTGTGGTTGGGAATACGCTGTATGTTGCAAATGTGGGTGATTCGAGAGCAGTGATTGCGGTGAAAGATGGTAATAGGATTATTGCAGAGGATTTGTCTTGTGACCAAACGCCGTTTAGGAAAGATGAGTATGAGAGAGTGAAGCAGTCTGGAGCCAGGGTTTTGAGTGTTGATCAAGTGGAAGGGCTTAAGGATCCAAGCATTCAGAGTTGGGGTGATGAAGAGAGTGAAGGGGGTGATCCTCCAAGGCTGTGGGTTCAAAATGGTATGTATCCTGGGACTGCATTTACAAGGAGCGTTGGTGATAGTACTGCTGAGAAGATTGGTGTTGTTGCAACTCCGGAAGTGTCTATGGTTCAGCTGACTCCCAATCATCTTTTCTTTGTCGTTGCAAGTGATGGGGTTTTCGAGTTCCTCTCAAGCCAAGCTGTTGTTAATATGGCAGCAAAATATTCAGATCCTCGGGATGCATGTGCTGCCATTGCTGGAGAATCATATAAGTTATGGTTGGAACATGAAAACCGAACAGATGATATAACAATTATCATTGTTCACATCAAAGGCTTGTCTAATTCAGCTGGTGGTGCTACAGATGGAACAGATGGGAGCACTAATAGAGCTGCAAGGAGGAGGAGAAAAGGGAATTCTGGGTCATCATTTAACAGCGGTTCGGAAGTTTTCCGTTCAGTGAGAAGTGATTCCTCAGAGATACAGTCTTGTCAGCTTGTGCTTTCGATGAATCGAAACCCAGCTATTGTTGTTCCATCTCCTACATGTCAAAGCCCTAGAAATAGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

426

Amino Acids

46.25

Weight (kDa)

5.08

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 79 - 320 1.4e-37 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 396
Acc65I GGTACC 1 cut(s) 308
AccB1I GGYRCC 1 cut(s) 308
AccIII TCCGGA 2 cut(s) 77, 820
AciI CCGC 3 cut(s) 27, 539, 1146
AclWI GGATC 6 cut(s) 50, 356, 668, 681, 713, 932
AcsI RAATTY 2 cut(s) 65, 1123
AfaI GTAC 3 cut(s) 170, 310, 790
AfeI AGCGCT 1 cut(s) 91
AfiI CCNNNNNNNGG 1 cut(s) 152
AflII CTTAAG 1 cut(s) 668
AflIII ACRYGT 1 cut(s) 1253
AgsI TTSAA 3 cut(s) 160, 254, 740
AjnI CCWGG 2 cut(s) 635, 754
AjuI GAANNNNNNNTTGG 2 cut(s) 842, 874
AluBI AGCT 9 cut(s) 250, 392, 427, 841, 908, 1058, 1100, 1204, 1232
AluI AGCT 9 cut(s) 250, 392, 427, 841, 908, 1058, 1100, 1204, 1232
Alw21I GWGCWC 1 cut(s) 1091
Alw26I GTCTC 1 cut(s) 66
AlwI GGATC 6 cut(s) 50, 356, 668, 681, 713, 932
AlwNI CAGNNNCTG 1 cut(s) 234
Ama87I CYCGRG 2 cut(s) 60, 942
Aor13HI TCCGGA 2 cut(s) 77, 820
Aor51HI AGCGCT 1 cut(s) 91
ApeKI GCWGC 5 cut(s) 103, 427, 923, 956, 1100
ApoI RAATTY 2 cut(s) 65, 1123
Asp700I GAANNNNTTC 1 cut(s) 1177
Asp718I GGTACC 1 cut(s) 308
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 2 cut(s) 80, 136
AsuC2I CCSGG 2 cut(s) 61, 62
AsuHPI GGTGA 5 cut(s) 530, 553, 707, 728, 794
AvaI CYCGRG 2 cut(s) 60, 942
AvaII GGWCC 2 cut(s) 80, 136
BamHI GGATCC 1 cut(s) 673
BanI GGYRCC 1 cut(s) 308
Bbv12I GWGCWC 1 cut(s) 1091
BbvI GCAGC 5 cut(s) 115, 414, 935, 943, 1087
BccI CCATC 6 cut(s) 284, 542, 872, 1067, 1076, 1252
BceAI ACGGC 4 cut(s) 67, 169, 483, 577
BcgI CGANNNNNNTGC 2 cut(s) 460, 494
BciT130I CCWGG 2 cut(s) 637, 756
BciVI GTATCC 1 cut(s) 762
BclI TGATCA 1 cut(s) 652
BcnI CCSGG 2 cut(s) 61, 62
BcoDI GTCTC 1 cut(s) 66
BfaI CTAG 1 cut(s) 1266
BfmI CTRYAG 2 cut(s) 85, 1068
BfoI RGCGCY 1 cut(s) 93
BfrI CTTAAG 1 cut(s) 668
BfuI GTATCC 1 cut(s) 762
BisI GCNGC 5 cut(s) 104, 428, 924, 957, 1101
BlsI GCNGC 5 cut(s) 105, 429, 925, 958, 1102
BmcAI AGTACT 1 cut(s) 790
Bme1390I CCNGG 4 cut(s) 61, 62, 637, 756
Bme18I GGWCC 2 cut(s) 80, 136
BmeT110I CYCGRG 2 cut(s) 60, 942
BmgT120I GGNCC 2 cut(s) 80, 136
BmiI GGNNCC 6 cut(s) 82, 138, 310, 463, 634, 675
BmrFI CCNGG 4 cut(s) 61, 62, 637, 756
BmrI ACTGGG 1 cut(s) 307
BmsI GCATC 1 cut(s) 937
BmuI ACTGGG 1 cut(s) 307
BplI GAGNNNNNCTC 2 cut(s) 881, 913
BpmI CTGGAG 2 cut(s) 651, 987
BpuEI CTTGAG 1 cut(s) 883
BpuMI CCSGG 2 cut(s) 61, 62
BsaJI CCNNGG 8 cut(s) 13, 59, 60, 465, 636, 725, 755, 941
BsaWI WCCGGW 2 cut(s) 77, 820
BsaXI ACNNNNNCTCC 4 cut(s) 624, 654, 766, 796
Bsc4I CCNNNNNNNGG 1 cut(s) 152
Bse1I ACTGG 2 cut(s) 313, 345
Bse3DI GCAATG 2 cut(s) 368, 960
BseAI TCCGGA 2 cut(s) 77, 820
BseBI CCWGG 2 cut(s) 637, 756
BseDI CCNNGG 8 cut(s) 13, 59, 60, 465, 636, 725, 755, 941
BseGI GGATG 1 cut(s) 952
BseLI CCNNNNNNNGG 1 cut(s) 152
BseMI GCAATG 2 cut(s) 368, 960
BseMII CTCAG 3 cut(s) 107, 786, 1197
BseNI ACTGG 2 cut(s) 313, 345
BseRI GAGGAG 2 cut(s) 1123, 1126
BseXI GCAGC 5 cut(s) 115, 414, 935, 943, 1087
BseYI CCCAGC 1 cut(s) 1228
Bsh1285I CGRYCG 1 cut(s) 31
BshNI GGYRCC 1 cut(s) 308
BsiEI CGRYCG 1 cut(s) 31
BsiHKAI GWGCWC 1 cut(s) 1091
BsiHKCI CYCGRG 2 cut(s) 60, 942
BsiSI CCGG 4 cut(s) 61, 78, 209, 821
BslFI GGGAC 2 cut(s) 122, 772
BslI CCNNNNNNNGG 1 cut(s) 152
BsmAI GTCTC 1 cut(s) 66
BsmFI GGGAC 2 cut(s) 122, 772
BsmI GAATGC 1 cut(s) 681
BsoBI CYCGRG 2 cut(s) 60, 942
Bsp1286I GDGCHC 1 cut(s) 1091
Bsp13I TCCGGA 2 cut(s) 77, 820
Bsp143I GATC 6 cut(s) 55, 361, 652, 673, 718, 937
Bsp19I CCATGG 1 cut(s) 13
BspACI CCGC 3 cut(s) 27, 539, 1146
BspCNI CTCAG 3 cut(s) 106, 787, 1196
BspEI TCCGGA 2 cut(s) 77, 820
BspLI GGNNCC 6 cut(s) 82, 138, 310, 463, 634, 675
BspPI GGATC 6 cut(s) 50, 356, 668, 681, 713, 932
BspT107I GGYRCC 1 cut(s) 308
BspTI CTTAAG 1 cut(s) 668
BsrDI GCAATG 2 cut(s) 368, 960
BsrI ACTGG 2 cut(s) 313, 345
BssECI CCNNGG 8 cut(s) 13, 59, 60, 465, 636, 725, 755, 941
BssMI GATC 6 cut(s) 55, 361, 652, 673, 718, 937
BssT1I CCWWGG 2 cut(s) 13, 725
Bst2UI CCWGG 2 cut(s) 637, 756
Bst4CI ACNGT 4 cut(s) 175, 230, 478, 1194
Bst6I CTCTTC 1 cut(s) 696
BstAFI CTTAAG 1 cut(s) 668
BstAPI GCANNNNNTGC 3 cut(s) 24, 536, 956
BstC8I GCNNGC 1 cut(s) 425
BstDEI CTNAG 3 cut(s) 93, 795, 1183
BstDSI CCRYGG 2 cut(s) 13, 465
BstEII GGTNACC 1 cut(s) 257
BstF5I GGATG 1 cut(s) 952
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 6 cut(s) 58, 364, 655, 676, 721, 940
BstMAI GTCTC 1 cut(s) 66
BstMBI GATC 6 cut(s) 55, 361, 652, 673, 718, 937
BstMCI CGRYCG 1 cut(s) 31
BstMWI GCNNNNNNNGC 5 cut(s) 24, 536, 956, 962, 1064
BstNI CCWGG 2 cut(s) 637, 756
BstNSI RCATGY 2 cut(s) 954, 1257
BstPI GGTNACC 1 cut(s) 257
BstSCI CCNGG 4 cut(s) 59, 60, 635, 754
BstSFI CTRYAG 2 cut(s) 85, 1068
BstV1I GCAGC 5 cut(s) 115, 414, 935, 943, 1087
BstX2I RGATCY 2 cut(s) 673, 937
BstXI CCANNNNNNTGG 2 cut(s) 278, 732
BstYI RGATCY 2 cut(s) 673, 937
BsuI GTATCC 1 cut(s) 762
BtgI CCRYGG 2 cut(s) 13, 465
BtsCI GGATG 1 cut(s) 952
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 4 cut(s) 171, 320, 537, 1173
Cac8I GCNNGC 1 cut(s) 425
CaiI CAGNNNCTG 1 cut(s) 234
CfoI GCGC 1 cut(s) 92
Cfr13I GGNCC 2 cut(s) 80, 136
Cfr9I CCCGGG 1 cut(s) 60
Csp6I GTAC 3 cut(s) 169, 309, 789
CviAII CATG 5 cut(s) 14, 296, 951, 995, 1254
CviQI GTAC 3 cut(s) 169, 309, 789
DdeI CTNAG 3 cut(s) 93, 795, 1183
DpnI GATC 6 cut(s) 57, 363, 654, 675, 720, 939
DpnII GATC 6 cut(s) 55, 361, 652, 673, 718, 937
Eam1104I CTCTTC 1 cut(s) 696
EarI CTCTTC 1 cut(s) 696
Eco130I CCWWGG 2 cut(s) 13, 725
Eco47I GGWCC 2 cut(s) 80, 136
Eco47III AGCGCT 1 cut(s) 91
Eco88I CYCGRG 2 cut(s) 60, 942
Eco91I GGTNACC 1 cut(s) 257
EcoO65I GGTNACC 1 cut(s) 257
EcoRI GAATTC 2 cut(s) 65, 1123
EcoRII CCWGG 2 cut(s) 635, 754
EcoT14I CCWWGG 2 cut(s) 13, 725
EcoT22I ATGCAT 1 cut(s) 952
ErhI CCWWGG 2 cut(s) 13, 725
FaeI CATG 5 cut(s) 17, 299, 954, 998, 1257
FaqI GGGAC 2 cut(s) 122, 772
FatI CATG 5 cut(s) 13, 295, 950, 994, 1253
FbaI TGATCA 1 cut(s) 652
Fnu4HI GCNGC 5 cut(s) 104, 428, 924, 957, 1101
FokI GGATG 1 cut(s) 959
Fsp4HI GCNGC 5 cut(s) 104, 428, 924, 957, 1101
FspBI CTAG 1 cut(s) 1266
GlaI GCGC 1 cut(s) 91
GluI GCNGC 5 cut(s) 104, 428, 924, 957, 1101
GsaI CCCAGC 1 cut(s) 1232
GsuI CTGGAG 2 cut(s) 651, 987
HaeII RGCGCY 1 cut(s) 93
HapII CCGG 4 cut(s) 61, 78, 209, 821
HhaI GCGC 1 cut(s) 92
Hin1II CATG 5 cut(s) 17, 299, 954, 998, 1257
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HindIII AAGCTT 1 cut(s) 390
HinfI GANTC 7 cut(s) 74, 203, 521, 844, 971, 1178, 1219
HpaII CCGG 4 cut(s) 61, 78, 209, 821
HphI GGTGA 5 cut(s) 530, 553, 707, 728, 794
Hpy166II GTNNAC 2 cut(s) 171, 1033
Hpy188I TCNGA 7 cut(s) 49, 71, 96, 687, 937, 1153, 1186
Hpy188III TCNNGA 7 cut(s) 78, 200, 224, 525, 630, 821, 944
Hpy8I GTNNAC 2 cut(s) 171, 1033
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 3 cut(s) 156, 656, 704
HpyCH4III ACNGT 4 cut(s) 175, 230, 478, 1194
HpyCH4V TGCA 8 cut(s) 430, 509, 566, 764, 815, 872, 950, 1103
HpyF10VI GCNNNNNNNGC 5 cut(s) 24, 536, 956, 962, 1064
HpyF3I CTNAG 3 cut(s) 93, 795, 1183
Hsp92II CATG 5 cut(s) 17, 299, 954, 998, 1257
HspAI GCGC 1 cut(s) 90
Kpn2I TCCGGA 2 cut(s) 77, 820
KpnI GGTACC 1 cut(s) 312
Ksp22I TGATCA 1 cut(s) 652
Kzo9I GATC 6 cut(s) 55, 361, 652, 673, 718, 937
LmnI GCTCC 3 cut(s) 632, 774, 1086
Lsp1109I GCAGC 5 cut(s) 115, 414, 935, 943, 1087
LweI GCATC 1 cut(s) 937
MaeI CTAG 1 cut(s) 1266
MaeIII GTNAC 3 cut(s) 191, 257, 581
MalI GATC 6 cut(s) 57, 363, 654, 675, 720, 939
MboI GATC 6 cut(s) 55, 361, 652, 673, 718, 937
MboII GAAGA 2 cut(s) 713, 811
MflI RGATCY 2 cut(s) 673, 937
MhlI GDGCHC 1 cut(s) 1091
MluCI AATT 8 cut(s) 65, 160, 302, 397, 441, 1020, 1051, 1123
MlyI GAGTC 3 cut(s) 68, 197, 838
MmeI TCCRAC 2 cut(s) 72, 969
Mph1103I ATGCAT 1 cut(s) 952
MroI TCCGGA 2 cut(s) 77, 820
MroXI GAANNNNTTC 1 cut(s) 1177
MseI TTAA 6 cut(s) 240, 330, 410, 669, 915, 1140
MslI CAYNNNNRTG 1 cut(s) 435
MspA1I CMGCKG 3 cut(s) 841, 1058, 1146
MspCI CTTAAG 1 cut(s) 668
MspI CCGG 4 cut(s) 61, 78, 209, 821
MspR9I CCNGG 4 cut(s) 61, 62, 637, 756
Mva1269I GAATGC 1 cut(s) 681
MvaI CCWGG 2 cut(s) 637, 756
MwoI GCNNNNNNNGC 5 cut(s) 24, 536, 956, 962, 1064
NciI CCSGG 2 cut(s) 61, 62
NcoI CCATGG 1 cut(s) 13
NdeII GATC 6 cut(s) 55, 361, 652, 673, 718, 937
NlaIII CATG 5 cut(s) 17, 299, 954, 998, 1257
NlaIV GGNNCC 6 cut(s) 82, 138, 310, 463, 634, 675
NmuCI GTSAC 1 cut(s) 581
NsiI ATGCAT 1 cut(s) 952
NspI RCATGY 2 cut(s) 954, 1257
PciI ACATGT 1 cut(s) 1253
PctI GAATGC 1 cut(s) 681
PdmI GAANNNNTTC 1 cut(s) 1177
PfeI GAWTC 4 cut(s) 521, 971, 1178, 1219
PkrI GCNGC 5 cut(s) 105, 429, 925, 958, 1102
PleI GAGTC 3 cut(s) 68, 197, 838
PpsI GAGTC 3 cut(s) 68, 197, 838
PscI ACATGT 1 cut(s) 1253
PsiI TTATAA 1 cut(s) 396
Psp6I CCWGG 2 cut(s) 635, 754
PspEI GGTNACC 1 cut(s) 257
PspFI CCCAGC 1 cut(s) 1228
PspGI CCWGG 2 cut(s) 635, 754
PspN4I GGNNCC 6 cut(s) 82, 138, 310, 463, 634, 675
PspPI GGNCC 2 cut(s) 80, 136
PstNI CAGNNNCTG 1 cut(s) 234
PsuI RGATCY 2 cut(s) 673, 937
PvuII CAGCTG 2 cut(s) 841, 1058
RsaI GTAC 3 cut(s) 170, 310, 790
RsaNI GTAC 3 cut(s) 169, 309, 789
RseI CAYNNNNRTG 1 cut(s) 435
SaqAI TTAA 6 cut(s) 240, 330, 410, 669, 915, 1140
SatI GCNGC 5 cut(s) 104, 428, 924, 957, 1101
Sau3AI GATC 6 cut(s) 55, 361, 652, 673, 718, 937
Sau96I GGNCC 2 cut(s) 80, 136
ScaI AGTACT 1 cut(s) 790
SchI GAGTC 3 cut(s) 68, 197, 838
ScrFI CCNGG 4 cut(s) 61, 62, 637, 756
SduI GDGCHC 1 cut(s) 1091
SfaNI GCATC 1 cut(s) 937
SfcI CTRYAG 2 cut(s) 85, 1068
SinI GGWCC 2 cut(s) 80, 136
SmaI CCCGGG 1 cut(s) 62
SmiMI CAYNNNNRTG 1 cut(s) 435
SmlI CTYRAG 2 cut(s) 668, 898
SmoI CTYRAG 2 cut(s) 668, 898
Sse9I AATT 8 cut(s) 65, 160, 302, 397, 441, 1020, 1051, 1123
SsiI CCGC 3 cut(s) 27, 539, 1146
SspI AATATT 1 cut(s) 932
SspMI CTAG 1 cut(s) 1266
StyD4I CCNGG 4 cut(s) 59, 60, 635, 754
StyI CCWWGG 2 cut(s) 13, 725
TaaI ACNGT 4 cut(s) 175, 230, 478, 1194
TaqI TCGA 4 cut(s) 524, 888, 1214, 1222
TasI AATT 8 cut(s) 65, 160, 302, 397, 441, 1020, 1051, 1123
TatI WGTACW 2 cut(s) 168, 788
TfiI GAWTC 4 cut(s) 521, 971, 1178, 1219
Tru1I TTAA 6 cut(s) 240, 330, 410, 669, 915, 1140
Tru9I TTAA 6 cut(s) 240, 330, 410, 669, 915, 1140
TscAI CASTG 4 cut(s) 178, 320, 537, 1173
TseFI GTSAC 1 cut(s) 581
TseI GCWGC 5 cut(s) 103, 427, 923, 956, 1100
Tsp45I GTSAC 1 cut(s) 581
TspDTI ATGAA 3 cut(s) 714, 1011, 1232
TspGWI ACGGA 1 cut(s) 1151
TspMI CCCGGG 1 cut(s) 60
TspRI CASTG 4 cut(s) 178, 320, 537, 1173
Vha464I CTTAAG 1 cut(s) 668
VpaK11BI GGWCC 2 cut(s) 80, 136
XapI RAATTY 2 cut(s) 65, 1123
XceI RCATGY 2 cut(s) 954, 1257
XmaI CCCGGG 1 cut(s) 60
XmnI GAANNNNTTC 1 cut(s) 1177
XspI CTAG 1 cut(s) 1266
ZrmI AGTACT 1 cut(s) 790
Zsp2I ATGCAT 1 cut(s) 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.