Rorug04G0159900

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007949
Physical Location & Seq
Reverse (-)
27856242 .. 27865265
9024 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0159900.1

Sequence Viewer

Length: 4284 bp
ATGAGTGATTGGGAAGAAAAAGAAACAAGAGGTGAAATCTTGAATTTGGTTTTGATTCTTTCTCATCGGATTGGTTTCAGGCAGACGAAGAACAGGGGGTTGGTAAGAGCTACACTCAAAACACTGAATCAGTTTTTGTCATTAGAACCAACCACCTCGTATACTGCTTTGATCCGTAGCTGGGGTCATAGAGAATCACTAATAGAGCTTACAAATCAAAAGGAGATCGTAGGAGATCTTTTCAACCAACCTCGACTTGCCTCTCAAAGACGAACTAAAGGGCGTCCTGCTCAAAGGACCAAAATTTGGAGTTTCAGTATGGAAAATTTTGAACAGCCTATGGACTTGAGCTCTGGAGGTGGCGATAGCAATGCAATCATCTTGCCAAATAAGAGAAAGAGGAAAAGGAAGGGTACTACTCAGGAGGGTAAGATACTTGGCACAAGAAAAACACCGAAGATGACCAAATCCCAGGAAAGGAAGTTGCAAAAGTTGGAGGTCGAGAAGGAAAAAGCCCTTTTGCTATCCAAGAGCATTGATGCCTTAGGGAAATATAAGCTTCCGGAGGGTGCATATTCTCTTTTGCAATCATCAAAAGATATTGGCCAGGTTGAATCAAAAAAGGAAAAACGTAGGAAGGCTGTACTATTCTCCAAAGCTGGATTAGAAGTTCCCTATACCAACCAACCATTAAAAAAGAAAGATGGTATGGATGCTTGTAGTGAAAGTGAACCTGAGTCGGAAAGAACTCAGTTAAGGCAGGATCTTGACAAAAGTGGTCAAGTACAACCGATGGTAATACAAAAGGAAATCCAAAAGACCACATCTGTATATTTGAACTCCTCGCAGGGGCTAGTCTATAGCAGAGGACATGCTACGAATGGTGGTCCTGCCGCTGGCTCACTGTCTAAAAATGTTATTTGCAACGTGTCTTTGCCAGAAAACATAGCTTCTAGTGTGAATGATGACCATGAAAGAACAGAGGCAATGGATAGAGTAAAAGGGAGCCCAAAGGTGACTTCAAGCAGGACAGGTGAATTTTCTGATTTTGCTGCTCCAAGGTCTCTGATCGCTCCAACTATAGTGCACGTATCAAGGCCGGTTGAAGTTGAAAACACAAGGAAGGATCTTCCAATAGTAATGATGGAACAGGAGATTATGGAGGCTGTTAATGACCATTCCACTGTTATTATATGTGGAGAAACAGGGTGTGGTAAAACCACTCAGGTTCCTCAGTTTCTTTTTGAAGCTGGCTTTGGTTCAAGCCACTCTTGTCATCGAAGCGGTATTATTGGTGTCACTCAACCTCGTCGTGTTGCAGTCCTTGCAACTGCTAAGCGAGTTGCATATGAGCTTGGTCTGCATTTAGGTAAAGAGGTTGGCTTTCAAGTTAGGTTTGACAAAAAATTTGGCGACTGTACCTCCATCAAGTTCATGACAGATGGAATATTACTACGCGAGCTTCAGAGTGATTTTTTGTTAAAGCGATACTCTATAATAATTCTTGATGAAGCTCATGAGCGGAGCTTGAGCACGGACATACTTATTGGAATGCTTTCTCGTGTAATTAGTACTCGCCAGGAAATCTATGCTAAGCAGCAAAGGGCTGTGCTTTCAGGAAAAACTATAGATCCTGGACAACAAGTTTTTCCATTAAAGCTTGTGCTGATGAGTGCCACCTTGCAAGTGGAAGACTTCATTTCTGGTAGAAAGTTGTTTCGTGATGTCCCGCCAGTTATAGAAGTTCCCACTAGACAATTTCCAGTAACCACTCACTTCTCAAAAAGAACTGGGACGGACTATGTTGGCCAAGCATATAAAAAAGTCTTGGCAATTCACAAGAGGCTACCCCGTGGGGGCATACTTGTCTTTGTCACTGGGCAGAAGGAAGTAGAGTTCTTGTGTCGAAAACTACGTAGAGCTTCAACAGAACTGGTCATGAAAACATCTAGAGGGAAAATTGGGTGTAATGCCACTGATGCTTCTAAAAAAGATGTTGAATTACTAGATATGAACGAGATTAATGAAGCATTTGAGGTTGAAGAAAATTTAGCCGACCATCAAATAGACAGGTTTAGCTCTGTTGATGAAGTTCTTGGTAATATATACGAGGATGATTCGTATGATTCAGACACAGACAGTGAAGTGGAAATTATTGAGGATGATGGGGGTTTATTGTTACACGATCCCCCAGAAATTGATGGTGTTGGTGCAGATGTTTTAGGAGAGACAGGGAGCATTGCTTCACTGAAAGCTGCTTTTGAAGCTTTGGCCGGTAAAACTGAACTGTACTCTAGTTCTGATGGGAAACAGCCAATTTCAGTTACTGTAGATGCTTACTTTAATAAATCCCATCCTAGTCTTGGGAAAAAGAGTGAGGTTGATATTGATAATTCCCCAGGTGGCTTGCATGTTCTCCCTCTTTATGCTATGCTGCCTGCGGCTTCTCAGCTTCGCGTATTTGAAGAAGTCAAGGAAGGAGATCGACTAGTTGTTGTAGCCACGAATGTGGCTGAGACTTCTTTAACCATACCTGGGGTAAAGTATGTCGTCGATACTGGCAGAGTAAAAGTGAAAAACTACAATTCCTCAAATGGTATGGAAACATATAACATAAAGTGGATAAGTAAGGCATCAGCTGCTCAACGTAAAGGAAGAGCCGGAAGAACGGGACCTGGGCACTGTTATCGTCTTTATTCTTCTGCAGTATATAATAATGAATTTCCCGACTTTTCTCCAGCTGAAATATTGAAAGTCCCTATTGATGGCGTTGTCCTTCTCATGAAATCTATGAATATTGAAAAGGTATCCAATTTTCCATTTCCAACTCCACCTGATGTTGCTGCCTTGGGTGAAGCAGAACGTTGCTTGAATGCCCTTGAAGCACTTGACAGCAATGGCAGATTAACACCTTTAGGGAAGGCCATGGCTCGTTACCCCATGAGTCCTCGCCACTCCAGGATGCTCCTTACGGTTATTCAAATCATGTACAACGAGAAAAGTTATTCCCGCAGTAAATTAATTCTAGCATATGCAGTTGCAGCAGCGGCTGCTTTGAGTTTGTCAAATCCTTTTGTGAGGCAGTTTGAAAATGACTCAAATAACCATGACTTGGATCAGGATGAAAATCTCAGCACTCCTGTGAACAATAAGGTGTTAGAGAAGCAAGAAAAGTTAAGAAGGAAGAAACTAAAAGAAACTGTAAAGGTGTTTCGTGAAAAATTTTCTAATCCTGTCAGTGATGCTCTGTCTGTAGCTTATGCTTTGCAGTGCTACGAACTTTCTAGGAGCCCACTAAAGTTCTGTGATGATAATGCCCTACACCCAAAAACGATGGAGGAAATGTCCAAGTTGAGAAAGCAACTGCTCCAACTTGTATTTAGTCAATGTGGTGTATCTGGCAGTGAGAAGGATTTTTCATGCATTTTTGGAAGTTTGGAAGATGTTGAACATGTTTGGAGGGCTTCCCATGATAAGAGTCCTCTTTCACTGTATGAGGAAGACCTCTTAGGCCAAGCTATCTGTGCTGGTTGGGCAGATAGGGTGGCGAAACGTATTAGAGGAACCTCAGGGTCATCTGAAGTAGATAGAAAAGTTCATGCAGTGCGGTATCAAGCCTGCATGGTTGAAGAGACTGTGTTCCTCCACCGTTGGTCATCTGTTTCTAATGCTGCCCCAGAGTTTTTGGTGTACACTGAGCTAATACAGACAAGACGTCCATACATGCATGGCGTTACTAGTGTAAAACCAGAATGGCTTGTAAAGTATGCTCCGTCTTTATGCACTTTCTCTGCAGCCTCATCCAATGCCAAACAGTATTATGAGCCTGTGACTGACCAAGTGTTACATGATGTCATTCCAGCCTTTGGTCCTCATCTATGGAAGCTTCCCCCACATAGTTTGCCTATTACTAACTATGTCTCTCGAGTGGCAGTTTTTGCTTATGCTTTGCTTGAAGGCCAGGTTTTGCCATGCTTGAGACGTGCTCGTGAGTTTATGAAGGCACCTCCAGCTAGTATTTTACGGCCAGAGGCAGAAGGGCAAAGACGGGCTGGAAATTTGTTAGCTAAGTTGAACATAAAAAAGATAGACAGCTGTGCTATGTTAAGAGAGATCTGGAAGGAGAATCCTAATGAATTGCATTCAGAAATCCAGGATTGGTTTAAGGAGAGTTTCCGCAAGAATTTGGCAGCTCTATGGTCAGACATGCTTAGGGAAGTTCTTTTAGAGCCGCAAGAACGGTTTTCAAAGAGGGAGATCAGAAGAATTGAAGTTAAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

1427

Amino Acids

159.72

Weight (kDa)

8.9

Isoelectric Point (pI)

47.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 802 - 891 4.2e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 953 - 980 3.5e-09 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 982 - 1084 2.8e-08 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1171 - 1259 6.3e-19 Oligonucleotide/oligosaccharide-binding (OB)-fold
DHX37_C PF23362 1326 - 1402 7e-17 DEAH helicase DHX37 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 3718
AccB1I GGYRCC 1 cut(s) 4003
AccB7I CCANNNNNTGG 3 cut(s) 306, 3112, 3866
AccBSI CCGCTC 1 cut(s) 1522
AccI GTMKAC 1 cut(s) 161
AccII CGCG 2 cut(s) 1458, 2457
AccIII TCCGGA 1 cut(s) 562
AclI AACGTT 1 cut(s) 2863
AclWI GGATC 6 cut(s) 166, 771, 1134, 1625, 2180, 3123
AcoI YGGCCR 4 cut(s) 604, 1807, 2271, 4025
AcuI CTGAAG 2 cut(s) 1448, 3600
AcyI GRCGYC 2 cut(s) 283, 3715
AfaI GTAC 8 cut(s) 415, 645, 786, 1420, 1573, 2291, 2990, 3692
AflIII ACRYGT 2 cut(s) 927, 3451
AhdI GACNNNNNGTC 1 cut(s) 3714
AhlI ACTAGT 2 cut(s) 2488, 3737
AjiI CACGTC 1 cut(s) 3983
AjuI GAANNNNNNNTTGG 6 cut(s) 83, 115, 1003, 1035, 1239, 1271
AleI CACNNNNGTG 2 cut(s) 2507, 3140
AloI GAACNNNNNNTCC 4 cut(s) 654, 686, 1880, 1912
Alw21I GWGCWC 4 cut(s) 353, 1089, 1535, 3988
Alw26I GTCTC 6 cut(s) 1068, 2222, 2510, 3626, 3925, 3973
Alw44I GTGCAC 1 cut(s) 1085
AlwI GGATC 6 cut(s) 166, 771, 1134, 1625, 2180, 3123
AlwNI CAGNNNCTG 2 cut(s) 2327, 3050
Ama87I CYCGRG 1 cut(s) 3924
Aor13HI TCCGGA 1 cut(s) 562
AoxI GGCC 8 cut(s) 604, 1097, 1807, 2271, 2922, 3511, 3958, 4025
ApaLI GTGCAC 1 cut(s) 1085
ArsI GACNNNNNNTTYG 2 cut(s) 1391, 1423
AseI ATTAAT 2 cut(s) 2022, 3020
Asp700I GAANNNNTTC 1 cut(s) 1555
AspS9I GGNCC 4 cut(s) 297, 887, 2672, 3869
AsuHPI GGTGA 4 cut(s) 44, 1027, 1046, 2864
AvaI CYCGRG 1 cut(s) 3924
AvaII GGWCC 4 cut(s) 297, 887, 2672, 3869
AxyI CCTNAGG 2 cut(s) 544, 3568
BaeGI GKGCMC 2 cut(s) 1089, 2682
BalI TGGCCA 2 cut(s) 606, 1809
BanI GGYRCC 1 cut(s) 4003
BanII GRGCYC 3 cut(s) 353, 1010, 3293
BauI CACGAG 2 cut(s) 1560, 3987
BbsI GAAGAC 2 cut(s) 1698, 3507
Bbv12I GWGCWC 4 cut(s) 353, 1089, 1535, 3988
BceAI ACGGC 1 cut(s) 4040
BcgI CGANNNNNNTGC 4 cut(s) 353, 387, 2669, 2703
BciVI GTATCC 1 cut(s) 2818
BcoDI GTCTC 6 cut(s) 1068, 2222, 2510, 3626, 3925, 3973
BcuI ACTAGT 2 cut(s) 2488, 3737
BfmI CTRYAG 7 cut(s) 859, 1080, 1626, 2328, 2703, 3252, 3792
BfuI GTATCC 1 cut(s) 2818
BglII AGATCT 2 cut(s) 235, 4113
BlpI GCTNAGC 2 cut(s) 1335, 1593
BmcAI AGTACT 1 cut(s) 1573
Bme18I GGWCC 4 cut(s) 297, 887, 2672, 3869
BmeRI GACNNNNNGTC 1 cut(s) 3714
BmeT110I CYCGRG 1 cut(s) 3924
BmgBI CACGTC 1 cut(s) 3983
BmgT120I GGNCC 4 cut(s) 297, 887, 2672, 3869
BmiI GGNNCC 6 cut(s) 1007, 1230, 2673, 3290, 3565, 4005
BmrI ACTGGG 2 cut(s) 1800, 1887
BmsI GCATC 7 cut(s) 529, 703, 1969, 2323, 2642, 2952, 3232
BmuI ACTGGG 2 cut(s) 1800, 1887
BpiI GAAGAC 2 cut(s) 1698, 3507
BplI GAGNNNNNCTC 4 cut(s) 99, 131, 3970, 4002
BpmI CTGGAG 4 cut(s) 375, 2721, 2941, 3993
Bpu10I CCTNAGC 1 cut(s) 4211
Bpu1102I GCTNAGC 2 cut(s) 1335, 1593
BpuEI CTTGAG 3 cut(s) 367, 1549, 3997
BsaAI YACGTR 2 cut(s) 1090, 1916
BsaBI GATNNNNATC 1 cut(s) 3126
BsaHI GRCGYC 2 cut(s) 283, 3715
BsaI GGTCTC 1 cut(s) 1068
BsaJI CCNNGG 8 cut(s) 471, 1058, 1852, 2398, 2534, 2675, 2847, 2925
BsaWI WCCGGW 1 cut(s) 562
BsaXI ACNNNNNCTCC 6 cut(s) 301, 331, 1406, 1436, 3329, 3359
Bse118I RCCGGY 2 cut(s) 1099, 2273
Bse1I ACTGG 6 cut(s) 1733, 1763, 1795, 1882, 1938, 2563
Bse21I CCTNAGG 2 cut(s) 544, 3568
Bse3DI GCAATG 4 cut(s) 376, 993, 2238, 2902
Bse8I GATNNNNATC 1 cut(s) 3126
BseAI TCCGGA 1 cut(s) 562
BseDI CCNNGG 8 cut(s) 471, 1058, 1852, 2398, 2534, 2675, 2847, 2925
BseGI GGATG 7 cut(s) 718, 2119, 2167, 2353, 2967, 3127, 3800
BseJI GATNNNNATC 1 cut(s) 3126
BseMI GCAATG 4 cut(s) 376, 993, 2238, 2902
BseNI ACTGG 6 cut(s) 1733, 1763, 1795, 1882, 1938, 2563
BseRI GAGGAG 1 cut(s) 832
BseSI GKGCMC 2 cut(s) 1089, 2682
BseYI CCCAGC 1 cut(s) 180
BsgI GTGCAG 1 cut(s) 2232
Bsh1236I CGCG 2 cut(s) 1458, 2457
BshFI GGCC 8 cut(s) 606, 1099, 1809, 2273, 2924, 3513, 3960, 4027
BshNI GGYRCC 1 cut(s) 4003
BsiHKAI GWGCWC 4 cut(s) 353, 1089, 1535, 3988
BsiHKCI CYCGRG 1 cut(s) 3924
BsiSI CCGG 4 cut(s) 563, 1100, 2274, 2661
BslFI GGGAC 4 cut(s) 1712, 1807, 2685, 2741
BsmAI GTCTC 6 cut(s) 1068, 2222, 2510, 3626, 3925, 3973
BsmBI CGTCTC 1 cut(s) 3973
BsmFI GGGAC 4 cut(s) 1712, 1807, 2685, 2741
BsmI GAATGC 3 cut(s) 1557, 2878, 4141
BsnI GGCC 8 cut(s) 606, 1099, 1809, 2273, 2924, 3513, 3960, 4027
Bso31I GGTCTC 1 cut(s) 1068
BsoBI CYCGRG 1 cut(s) 3924
Bsp1286I GDGCHC 7 cut(s) 353, 1010, 1089, 1535, 2682, 3293, 3988
Bsp13I TCCGGA 1 cut(s) 562
Bsp1407I TGTACA 2 cut(s) 2988, 3690
Bsp1720I GCTNAGC 2 cut(s) 1335, 1593
Bsp19I CCATGG 1 cut(s) 2925
BspANI GGCC 8 cut(s) 606, 1099, 1809, 2273, 2924, 3513, 3960, 4027
BspEI TCCGGA 1 cut(s) 562
BspFNI CGCG 2 cut(s) 1458, 2457
BspHI TCATGA 4 cut(s) 1434, 1516, 1938, 2781
BspLI GGNNCC 6 cut(s) 1007, 1230, 2673, 3290, 3565, 4005
BspMAI CTGCAG 2 cut(s) 2707, 3796
BspPI GGATC 6 cut(s) 166, 771, 1134, 1625, 2180, 3123
BspQI GCTCTTC 1 cut(s) 2650
BspT107I GGYRCC 1 cut(s) 4003
BspTNI GGTCTC 1 cut(s) 1068
BsrBI CCGCTC 1 cut(s) 1522
BsrDI GCAATG 4 cut(s) 376, 993, 2238, 2902
BsrFI RCCGGY 2 cut(s) 1099, 2273
BsrGI TGTACA 2 cut(s) 2988, 3690
BsrI ACTGG 6 cut(s) 1733, 1763, 1795, 1882, 1938, 2563
BssAI RCCGGY 2 cut(s) 1099, 2273
BssECI CCNNGG 8 cut(s) 471, 1058, 1852, 2398, 2534, 2675, 2847, 2925
BssNAI GTATAC 1 cut(s) 162
BssNI GRCGYC 2 cut(s) 283, 3715
BssSI CACGAG 2 cut(s) 1560, 3987
BssT1I CCWWGG 3 cut(s) 1058, 2847, 2925
Bst1107I GTATAC 1 cut(s) 162
Bst2BI CACGAG 2 cut(s) 1560, 3987
Bst6I CTCTTC 2 cut(s) 2650, 3624
BstACI GRCGYC 2 cut(s) 283, 3715
BstAPI GCANNNNNTGC 4 cut(s) 1325, 2639, 3050, 3938
BstAUI TGTACA 2 cut(s) 2988, 3690
BstBAI YACGTR 2 cut(s) 1090, 1916
BstC8I GCNNGC 6 cut(s) 898, 1252, 1460, 2408, 2439, 3619
BstDSI CCRYGG 2 cut(s) 1852, 2925
BstF5I GGATG 7 cut(s) 718, 2119, 2167, 2353, 2967, 3127, 3800
BstFNI CGCG 2 cut(s) 1458, 2457
BstMAI GTCTC 6 cut(s) 1068, 2222, 2510, 3626, 3925, 3973
BstNSI RCATGY 5 cut(s) 875, 2414, 3455, 3727, 4210
BstSFI CTRYAG 7 cut(s) 859, 1080, 1626, 2328, 2703, 3252, 3792
BstSLI GKGCMC 2 cut(s) 1089, 2682
BstSNI TACGTA 1 cut(s) 1916
BstUI CGCG 2 cut(s) 1458, 2457
BstV2I GAAGAC 2 cut(s) 1698, 3507
BstX2I RGATCY 5 cut(s) 235, 763, 1126, 1630, 4113
BstXI CCANNNNNNTGG 1 cut(s) 2509
BstYI RGATCY 5 cut(s) 235, 763, 1126, 1630, 4113
BstZ17I GTATAC 1 cut(s) 162
Bsu36I CCTNAGG 2 cut(s) 544, 3568
BsuI GTATCC 1 cut(s) 2818
BsuRI GGCC 8 cut(s) 606, 1099, 1809, 2273, 2924, 3513, 3960, 4027
BtgI CCRYGG 2 cut(s) 1852, 2925
BtrI CACGTC 1 cut(s) 3983
BtsCI GGATG 7 cut(s) 718, 2119, 2167, 2353, 2967, 3127, 3800
BtsI GCAGTG 3 cut(s) 3275, 3409, 3609
Cac8I GCNNGC 6 cut(s) 898, 1252, 1460, 2408, 2439, 3619
CaiI CAGNNNCTG 2 cut(s) 2327, 3050
CciI TCATGA 4 cut(s) 1434, 1516, 1938, 2781
Cfr10I RCCGGY 2 cut(s) 1099, 2273
Cfr13I GGNCC 4 cut(s) 297, 887, 2672, 3869
CseI GACGC 1 cut(s) 272
Csp6I GTAC 8 cut(s) 414, 644, 785, 1419, 1572, 2290, 2989, 3691
CspCI CAANNNNNGTGG 2 cut(s) 3882, 3917
CviQI GTAC 8 cut(s) 414, 644, 785, 1419, 1572, 2290, 2989, 3691
DriI GACNNNNNGTC 1 cut(s) 3714
EaeI YGGCCR 4 cut(s) 604, 1807, 2271, 4025
Eam1104I CTCTTC 2 cut(s) 2650, 3624
Eam1105I GACNNNNNGTC 1 cut(s) 3714
EarI CTCTTC 2 cut(s) 2650, 3624
Ecl136II GAGCTC 1 cut(s) 351
Eco105I TACGTA 1 cut(s) 1916
Eco130I CCWWGG 3 cut(s) 1058, 2847, 2925
Eco24I GRGCYC 3 cut(s) 353, 1010, 3293
Eco31I GGTCTC 1 cut(s) 1068
Eco47I GGWCC 4 cut(s) 297, 887, 2672, 3869
Eco53kI GAGCTC 1 cut(s) 351
Eco57I CTGAAG 2 cut(s) 1448, 3600
Eco81I CCTNAGG 2 cut(s) 544, 3568
Eco88I CYCGRG 1 cut(s) 3924
EcoICRI GAGCTC 1 cut(s) 351
EcoO109I RGGNCCY 1 cut(s) 2672
EcoT14I CCWWGG 3 cut(s) 1058, 2847, 2925
EcoT22I ATGCAT 2 cut(s) 3425, 3729
EcoT38I GRGCYC 3 cut(s) 353, 1010, 3293
ErhI CCWWGG 3 cut(s) 1058, 2847, 2925
Esp3I CGTCTC 1 cut(s) 3973
FalI AAGNNNNNCTT 2 cut(s) 1255, 1287
FaqI GGGAC 4 cut(s) 1712, 1807, 2685, 2741
FauI CCCGC 2 cut(s) 1737, 3017
FauNDI CATATG 2 cut(s) 1348, 3031
FblI GTMKAC 1 cut(s) 161
FokI GGATG 7 cut(s) 725, 2126, 2174, 2340, 2974, 3134, 3787
FriOI GRGCYC 3 cut(s) 353, 1010, 3293
GsaI CCCAGC 1 cut(s) 184
GsuI CTGGAG 4 cut(s) 375, 2721, 2941, 3993
HaeIII GGCC 8 cut(s) 606, 1099, 1809, 2273, 2924, 3513, 3960, 4027
HapII CCGG 4 cut(s) 563, 1100, 2274, 2661
HgaI GACGC 1 cut(s) 272
Hin1I GRCGYC 2 cut(s) 283, 3715
HindIII AAGCTT 4 cut(s) 557, 1658, 2265, 3884
HpaII CCGG 4 cut(s) 563, 1100, 2274, 2661
HphI GGTGA 4 cut(s) 44, 1027, 1046, 2864
Hpy166II GTNNAC 6 cut(s) 162, 731, 1087, 3145, 3691, 3693
Hpy8I GTNNAC 6 cut(s) 162, 731, 1087, 3145, 3691, 3693
Hpy99I CGWCG 2 cut(s) 1314, 2555
HpyCH4IV ACGT 9 cut(s) 631, 927, 1089, 1915, 2647, 2863, 3553, 3715, 3982
HpySE526I ACGT 9 cut(s) 631, 927, 1089, 1915, 2647, 2863, 3553, 3715, 3982
Hsp92I GRCGYC 2 cut(s) 283, 3715
Kpn2I TCCGGA 1 cut(s) 562
LguI GCTCTTC 1 cut(s) 2650
LmnI GCTCC 9 cut(s) 1005, 1060, 1078, 1524, 2235, 2970, 3288, 3372, 3775
LweI GCATC 7 cut(s) 529, 703, 1969, 2323, 2642, 2952, 3232
MaeII ACGT 9 cut(s) 631, 927, 1089, 1915, 2647, 2863, 3553, 3715, 3982
MbiI CCGCTC 1 cut(s) 1522
MflI RGATCY 5 cut(s) 235, 763, 1126, 1630, 4113
MhlI GDGCHC 7 cut(s) 353, 1010, 1089, 1535, 2682, 3293, 3988
MlsI TGGCCA 2 cut(s) 606, 1809
MluNI TGGCCA 2 cut(s) 606, 1809
MlyI GAGTC 4 cut(s) 746, 2953, 3089, 3487
MmeI TCCRAC 5 cut(s) 474, 720, 1100, 2849, 3394
Mox20I TGGCCA 2 cut(s) 606, 1809
Mph1103I ATGCAT 2 cut(s) 3425, 3729
MroI TCCGGA 1 cut(s) 562
MroXI GAANNNNTTC 1 cut(s) 1555
MscI TGGCCA 2 cut(s) 606, 1809
MslI CAYNNNNRTG 2 cut(s) 2507, 3140
Msp20I TGGCCA 2 cut(s) 606, 1809
MspA1I CMGCKG 5 cut(s) 896, 2639, 2741, 3047, 4095
MspI CCGG 4 cut(s) 563, 1100, 2274, 2661
Mva1269I GAATGC 3 cut(s) 1557, 2878, 4141
MvnI CGCG 2 cut(s) 1458, 2457
NcoI CCATGG 1 cut(s) 2925
NdeI CATATG 2 cut(s) 1348, 3031
NlaIV GGNNCC 6 cut(s) 1007, 1230, 2673, 3290, 3565, 4005
NmuCI GTSAC 4 cut(s) 1015, 1297, 1873, 3829
NsiI ATGCAT 2 cut(s) 3425, 3729
NspI RCATGY 5 cut(s) 875, 2414, 3455, 3727, 4210
OliI CACNNNNGTG 2 cut(s) 2507, 3140
PaeR7I CTCGAG 1 cut(s) 3924
PagI TCATGA 4 cut(s) 1434, 1516, 1938, 2781
PciI ACATGT 1 cut(s) 3451
PciSI GCTCTTC 1 cut(s) 2650
PcsI WCGNNNNNNNCGW 1 cut(s) 1912
PctI GAATGC 3 cut(s) 1557, 2878, 4141
PdmI GAANNNNTTC 1 cut(s) 1555
PfeI GAWTC 7 cut(s) 55, 127, 194, 614, 2117, 2126, 4126
PflMI CCANNNNNTGG 3 cut(s) 306, 3112, 3866
PfoI TCCNGGA 3 cut(s) 1633, 2957, 4152
PleI GAGTC 4 cut(s) 745, 2952, 3089, 3486
PpsI GAGTC 4 cut(s) 745, 2952, 3089, 3486
Ppu21I YACGTR 2 cut(s) 1090, 1916
PpuMI RGGWCCY 1 cut(s) 2672
PscI ACATGT 1 cut(s) 3451
PshBI ATTAAT 2 cut(s) 2022, 3020
Psp124BI GAGCTC 1 cut(s) 353
Psp1406I AACGTT 1 cut(s) 2863
Psp5II RGGWCCY 1 cut(s) 2672
PspFI CCCAGC 1 cut(s) 180
PspN4I GGNNCC 6 cut(s) 1007, 1230, 2673, 3290, 3565, 4005
PspPI GGNCC 4 cut(s) 297, 887, 2672, 3869
PspPPI RGGWCCY 1 cut(s) 2672
PstI CTGCAG 2 cut(s) 2707, 3796
PstNI CAGNNNCTG 2 cut(s) 2327, 3050
PsuI RGATCY 5 cut(s) 235, 763, 1126, 1630, 4113
PvuII CAGCTG 3 cut(s) 2639, 2741, 4095
RsaI GTAC 8 cut(s) 415, 645, 786, 1420, 1573, 2291, 2990, 3692
RsaNI GTAC 8 cut(s) 414, 644, 785, 1419, 1572, 2290, 2989, 3691
RseI CAYNNNNRTG 2 cut(s) 2507, 3140
SacI GAGCTC 1 cut(s) 353
SapI GCTCTTC 1 cut(s) 2650
Sau96I GGNCC 4 cut(s) 297, 887, 2672, 3869
ScaI AGTACT 1 cut(s) 1573
SchI GAGTC 4 cut(s) 746, 2953, 3089, 3487
SduI GDGCHC 7 cut(s) 353, 1010, 1089, 1535, 2682, 3293, 3988
SfaNI GCATC 7 cut(s) 529, 703, 1969, 2323, 2642, 2952, 3232
SfcI CTRYAG 7 cut(s) 859, 1080, 1626, 2328, 2703, 3252, 3792
Sfr274I CTCGAG 1 cut(s) 3924
SinI GGWCC 4 cut(s) 297, 887, 2672, 3869
SlaI CTCGAG 1 cut(s) 3924
SmiMI CAYNNNNRTG 2 cut(s) 2507, 3140
SmlI CTYRAG 4 cut(s) 346, 1528, 3924, 3976
SmoI CTYRAG 4 cut(s) 346, 1528, 3924, 3976
SnaBI TACGTA 1 cut(s) 1916
SpeI ACTAGT 2 cut(s) 2488, 3737
SspI AATATT 3 cut(s) 1449, 2748, 2797
SstI GAGCTC 1 cut(s) 353
StyI CCWWGG 3 cut(s) 1058, 2847, 2925
TaiI ACGT 9 cut(s) 634, 930, 1092, 1918, 2650, 2866, 3556, 3718, 3985
TaqI TCGA 7 cut(s) 253, 501, 1279, 1906, 2485, 2553, 3925
TatI WGTACW 6 cut(s) 643, 784, 1571, 2289, 2988, 3690
TauI GCSGC 4 cut(s) 896, 2444, 3050, 4234
TfiI GAWTC 7 cut(s) 55, 127, 194, 614, 2117, 2126, 4126
TseFI GTSAC 4 cut(s) 1015, 1297, 1873, 3829
Tsp45I GTSAC 4 cut(s) 1015, 1297, 1873, 3829
TspGWI ACGGA 4 cut(s) 164, 1550, 1811, 3762
Van91I CCANNNNNTGG 3 cut(s) 306, 3112, 3866
VneI GTGCAC 1 cut(s) 1085
VpaK11BI GGWCC 4 cut(s) 297, 887, 2672, 3869
VspI ATTAAT 2 cut(s) 2022, 3020
XbaI TCTAGA 1 cut(s) 1949
XceI RCATGY 5 cut(s) 875, 2414, 3455, 3727, 4210
XcmI CCANNNNNNNNNTGG 2 cut(s) 1684, 2360
XhoI CTCGAG 1 cut(s) 3924
XmiI GTMKAC 1 cut(s) 161
XmnI GAANNNNTTC 1 cut(s) 1555
ZraI GACGTC 1 cut(s) 3716
ZrmI AGTACT 1 cut(s) 1573
Zsp2I ATGCAT 2 cut(s) 3425, 3729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.