pycom16g15100

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007949
Physical Location & Seq
Reverse (-)
11092887 .. 11095567
2681 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g15100.4

Sequence Viewer

Length: 1275 bp
ATGGGTTGTGTCCATGGCAAGTGTTGCGGCCGGTACCCCCAGTCTTCCGATGGTGATCTACGGGACTTCAGAGATGCTGGTCCGTACAGCGCTCAGAGGAAGAACATACTCTCAGAGAGGTCATTGGAATTCGTCCCTGTTCCTTCACACGATTATCGTTTAGAGTACTCTTTGTTAACGCAGCGCGGCTACTATCCCGACTCGCCGGATAAGGAAAACCAGGACAGTTTCTGTGTTAGAACGCAAGTCCAAGGTAACCCAAATATCCATTTCTTTGGTGTGTTTGATGGGCATGGCCAATTGGGTACCCAGTGTTCTAATTTTGTTAAGGACAGGTTAGTAGAGATACTAGCGAATGATCCCACATTGTCAGCTGACCCTGTGAAAGCTTACAATTCTGCTTTTCTAACGACGAATTATGAGTTGCATAATAGTGAGATTGATGATACTATGAGTGGTACTACGGCGATAACTGTGCTTGTTATTGGGGATACCCTTTATGTCGCAAATGTGGGTGATTCGAGAGCAGTAATTGCAGTGAAAGATGGTAATAGAATTATTGCAGAGGACTTGTCTTCTGACCAAACGCCGTTTAGGAAAGACGAATGTGAGAGAGTGAAGCTGTGTGGGGCCAGAGTTCTGAGTGTTGATCAAGTGGAAGGGCTGAAGGATCCAGATATCCAGTCGTGGGGTGATGAAGAGAGTGAAGGTGGTGATCCTCCAAGGTTGTGGGTTCCAGCAGGGATGTATCCTGGAACTGCGTTTACAAGGAGTGTAGGTGATAGTACAGCTGAGACGATTGGTGTGGTTGCTACTCCAGAGGTGTCAATAGTTAAGCTGACACCTAATCATCTCTTCTTTGTTGTTGCAAGTGATGGAGTTTTCGAGTTTCTCTCAAGCCAAGCTGTGGTTAATATGGCGGCAAGATATCCAGATCCTCGAGATGCATGTGCTGCCATTGCAGGAGAATCATACAAATTATGGTTGGAACATGAAAACCGGACAGATGATATAACAATAATCATTGTTCACATCAAAGGCTTGTCCAATTCTGCTGGTGGTGCTGCAGATGAAGCAAGTGGGGCCAATAATAGGCCAGCAACACGAGTGAGAAAAACTTCAGAGTCATCTGTTACCACTGGGTCGGAAGTTTACCGCTCTGTGAGAAGTGATTCCTCAGAAGTACAGTCTTGTCAGCTTGTGCTTTCGATGAGTCGAAGCCCAGCTATTGTTGTTCCATCTCCTTCATGTCAGAGATCTTTGAGTTGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

425

Amino Acids

46.01

Weight (kDa)

4.89

Isoelectric Point (pI)

45.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 33, 305
AccB1I GGYRCC 2 cut(s) 33, 305
AccB7I CCANNNNNTGG 1 cut(s) 907
AccBSI CCGCTC 1 cut(s) 1158
AccII CGCG 1 cut(s) 186
AciI CCGC 4 cut(s) 27, 186, 920, 1156
AclWI GGATC 5 cut(s) 353, 665, 678, 710, 929
AcoI YGGCCR 2 cut(s) 28, 295
AcsI RAATTY 1 cut(s) 128
AcuI CTGAAG 3 cut(s) 52, 686, 1104
AfaI GTAC 7 cut(s) 35, 86, 167, 307, 460, 787, 1185
AfeI AGCGCT 1 cut(s) 91
AfiI CCNNNNNNNGG 3 cut(s) 688, 907, 1092
AjnI CCWGG 2 cut(s) 219, 751
AluBI AGCT 8 cut(s) 374, 389, 622, 791, 838, 905, 1198, 1226
AluI AGCT 8 cut(s) 374, 389, 622, 791, 838, 905, 1198, 1226
Alw26I GTCTC 1 cut(s) 788
AlwI GGATC 5 cut(s) 353, 665, 678, 710, 929
AlwNI CAGNNNCTG 1 cut(s) 231
Ama87I CYCGRG 1 cut(s) 939
Aor51HI AGCGCT 1 cut(s) 91
AoxI GGCC 5 cut(s) 28, 295, 630, 1083, 1094
ApeKI GCWGC 3 cut(s) 181, 953, 1064
ApoI RAATTY 1 cut(s) 128
Asp700I GAANNNNTTC 2 cut(s) 1117, 1171
Asp718I GGTACC 2 cut(s) 33, 305
AspLEI GCGC 2 cut(s) 92, 186
AspS9I GGNCC 3 cut(s) 80, 630, 1083
AsuHPI GGTGA 5 cut(s) 65, 527, 704, 725, 791
AvaI CYCGRG 1 cut(s) 939
AvaII GGWCC 1 cut(s) 80
BalI TGGCCA 1 cut(s) 297
BamHI GGATCC 1 cut(s) 670
BanI GGYRCC 2 cut(s) 33, 305
BauI CACGAG 1 cut(s) 1104
BbsI GAAGAC 2 cut(s) 36, 567
BbvI GCAGC 3 cut(s) 193, 940, 1051
BccI CCATC 5 cut(s) 44, 281, 539, 869, 1246
BceAI ACGGC 2 cut(s) 480, 574
BcgI CGANNNNNNTGC 2 cut(s) 457, 491
BciT130I CCWGG 2 cut(s) 221, 753
BciVI GTATCC 2 cut(s) 484, 759
BclI TGATCA 1 cut(s) 649
BcoDI GTCTC 1 cut(s) 788
BfaI CTAG 1 cut(s) 350
BfmI CTRYAG 1 cut(s) 1065
BfoI RGCGCY 1 cut(s) 93
BfuI GTATCC 2 cut(s) 484, 759
BglII AGATCT 1 cut(s) 1256
BisI GCNGC 6 cut(s) 28, 182, 187, 921, 954, 1065
BlsI GCNGC 6 cut(s) 29, 183, 188, 922, 955, 1066
BmcAI AGTACT 1 cut(s) 167
Bme1390I CCNGG 2 cut(s) 221, 753
Bme18I GGWCC 1 cut(s) 80
BmeT110I CYCGRG 1 cut(s) 939
BmgT120I GGNCC 3 cut(s) 80, 630, 1083
BmiI GGNNCC 6 cut(s) 35, 307, 631, 672, 735, 1084
BmrFI CCNGG 2 cut(s) 221, 753
BmrI ACTGGG 3 cut(s) 34, 304, 1149
BmsI GCATC 2 cut(s) 64, 934
BmuI ACTGGG 3 cut(s) 34, 304, 1149
BpiI GAAGAC 2 cut(s) 36, 567
BplI GAGNNNNNCTC 2 cut(s) 878, 910
BpmI CTGGAG 1 cut(s) 801
BpuEI CTTGAG 1 cut(s) 880
BsaBI GATNNNNATC 1 cut(s) 54
BsaJI CCNNGG 3 cut(s) 13, 250, 722
BsaWI WCCGGW 1 cut(s) 999
BsaXI ACNNNNNCTCC 2 cut(s) 763, 793
Bsc4I CCNNNNNNNGG 3 cut(s) 688, 907, 1092
Bse118I RCCGGY 1 cut(s) 30
Bse1I ACTGG 4 cut(s) 40, 310, 682, 1144
Bse3DI GCAATG 1 cut(s) 957
Bse8I GATNNNNATC 1 cut(s) 54
BseBI CCWGG 2 cut(s) 221, 753
BseDI CCNNGG 3 cut(s) 13, 250, 722
BseGI GGATG 1 cut(s) 750
BseJI GATNNNNATC 1 cut(s) 54
BseLI CCNNNNNNNGG 3 cut(s) 688, 907, 1092
BseMI GCAATG 1 cut(s) 957
BseMII CTCAG 5 cut(s) 107, 126, 632, 783, 1191
BseNI ACTGG 4 cut(s) 40, 310, 682, 1144
BseX3I CGGCCG 1 cut(s) 28
BseXI GCAGC 3 cut(s) 193, 940, 1051
BseYI CCCAGC 1 cut(s) 1222
Bsh1236I CGCG 1 cut(s) 186
Bsh1285I CGRYCG 1 cut(s) 31
BshFI GGCC 5 cut(s) 30, 297, 632, 1085, 1096
BshNI GGYRCC 2 cut(s) 33, 305
BsiEI CGRYCG 1 cut(s) 31
BsiHKCI CYCGRG 1 cut(s) 939
BsiSI CCGG 3 cut(s) 31, 206, 1000
BslFI GGGAC 2 cut(s) 77, 119
BslI CCNNNNNNNGG 3 cut(s) 688, 907, 1092
BsmAI GTCTC 1 cut(s) 788
BsmBI CGTCTC 1 cut(s) 788
BsmFI GGGAC 2 cut(s) 77, 119
BsnI GGCC 5 cut(s) 30, 297, 632, 1085, 1096
BsoBI CYCGRG 1 cut(s) 939
Bsp143I GATC 7 cut(s) 55, 358, 649, 670, 715, 934, 1256
Bsp19I CCATGG 1 cut(s) 13
BspACI CCGC 4 cut(s) 27, 186, 920, 1156
BspANI GGCC 5 cut(s) 30, 297, 632, 1085, 1096
BspCNI CTCAG 5 cut(s) 106, 125, 633, 784, 1190
BspFNI CGCG 1 cut(s) 186
BspLI GGNNCC 6 cut(s) 35, 307, 631, 672, 735, 1084
BspMAI CTGCAG 1 cut(s) 1069
BspPI GGATC 5 cut(s) 353, 665, 678, 710, 929
BspT107I GGYRCC 2 cut(s) 33, 305
BsrBI CCGCTC 1 cut(s) 1158
BsrDI GCAATG 1 cut(s) 957
BsrFI RCCGGY 1 cut(s) 30
BsrI ACTGG 4 cut(s) 40, 310, 682, 1144
BssAI RCCGGY 1 cut(s) 30
BssECI CCNNGG 3 cut(s) 13, 250, 722
BssMI GATC 7 cut(s) 55, 358, 649, 670, 715, 934, 1256
BssSI CACGAG 1 cut(s) 1104
BssT1I CCWWGG 3 cut(s) 13, 250, 722
Bst2BI CACGAG 1 cut(s) 1104
Bst2UI CCWGG 2 cut(s) 221, 753
Bst4CI ACNGT 3 cut(s) 227, 475, 1188
Bst6I CTCTTC 2 cut(s) 693, 860
BstAPI GCANNNNNTGC 3 cut(s) 24, 533, 953
BstC8I GCNNGC 1 cut(s) 1098
BstDEI CTNAG 5 cut(s) 93, 112, 641, 792, 1177
BstDSI CCRYGG 1 cut(s) 13
BstEII GGTNACC 1 cut(s) 254
BstF5I GGATG 1 cut(s) 750
BstFNI CGCG 1 cut(s) 186
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 2 cut(s) 92, 186
BstKTI GATC 7 cut(s) 58, 361, 652, 673, 718, 937, 1259
BstMAI GTCTC 1 cut(s) 788
BstMBI GATC 7 cut(s) 55, 358, 649, 670, 715, 934, 1256
BstMCI CGRYCG 1 cut(s) 31
BstMWI GCNNNNNNNGC 7 cut(s) 24, 533, 953, 959, 1061, 1073, 1082
BstNI CCWGG 2 cut(s) 221, 753
BstNSI RCATGY 1 cut(s) 951
BstPI GGTNACC 1 cut(s) 254
BstSCI CCNGG 2 cut(s) 219, 751
BstSFI CTRYAG 1 cut(s) 1065
BstUI CGCG 1 cut(s) 186
BstV1I GCAGC 3 cut(s) 193, 940, 1051
BstV2I GAAGAC 2 cut(s) 36, 567
BstX2I RGATCY 3 cut(s) 670, 934, 1256
BstXI CCANNNNNNTGG 2 cut(s) 275, 729
BstYI RGATCY 3 cut(s) 670, 934, 1256
BstZI CGGCCG 1 cut(s) 28
BsuI GTATCC 2 cut(s) 484, 759
BsuRI GGCC 5 cut(s) 30, 297, 632, 1085, 1096
BtgI CCRYGG 1 cut(s) 13
BtsCI GGATG 1 cut(s) 750
BtsI GCAGTG 1 cut(s) 543
BtsIMutI CAGTG 3 cut(s) 317, 543, 1137
Cac8I GCNNGC 1 cut(s) 1098
CaiI CAGNNNCTG 1 cut(s) 231
CfoI GCGC 2 cut(s) 92, 186
Cfr10I RCCGGY 1 cut(s) 30
Cfr13I GGNCC 3 cut(s) 80, 630, 1083
Csp6I GTAC 7 cut(s) 34, 85, 166, 306, 459, 786, 1184
CviAII CATG 5 cut(s) 14, 293, 948, 992, 1248
CviQI GTAC 7 cut(s) 34, 85, 166, 306, 459, 786, 1184
DdeI CTNAG 5 cut(s) 93, 112, 641, 792, 1177
DpnI GATC 7 cut(s) 57, 360, 651, 672, 717, 936, 1258
DpnII GATC 7 cut(s) 55, 358, 649, 670, 715, 934, 1256
EaeI YGGCCR 2 cut(s) 28, 295
EagI CGGCCG 1 cut(s) 28
Eam1104I CTCTTC 2 cut(s) 693, 860
EarI CTCTTC 2 cut(s) 693, 860
EclXI CGGCCG 1 cut(s) 28
Eco130I CCWWGG 3 cut(s) 13, 250, 722
Eco32I GATATC 2 cut(s) 679, 929
Eco47I GGWCC 1 cut(s) 80
Eco47III AGCGCT 1 cut(s) 91
Eco52I CGGCCG 1 cut(s) 28
Eco57I CTGAAG 3 cut(s) 52, 686, 1104
Eco88I CYCGRG 1 cut(s) 939
Eco91I GGTNACC 1 cut(s) 254
EcoO65I GGTNACC 1 cut(s) 254
EcoRI GAATTC 1 cut(s) 128
EcoRII CCWGG 2 cut(s) 219, 751
EcoRV GATATC 2 cut(s) 679, 929
EcoT14I CCWWGG 3 cut(s) 13, 250, 722
EcoT22I ATGCAT 1 cut(s) 949
ErhI CCWWGG 3 cut(s) 13, 250, 722
Esp3I CGTCTC 1 cut(s) 788
FaeI CATG 5 cut(s) 17, 296, 951, 995, 1251
FaqI GGGAC 2 cut(s) 77, 119
FatI CATG 5 cut(s) 13, 292, 947, 991, 1247
FbaI TGATCA 1 cut(s) 649
Fnu4HI GCNGC 6 cut(s) 28, 182, 187, 921, 954, 1065
FokI GGATG 1 cut(s) 757
Fsp4HI GCNGC 6 cut(s) 28, 182, 187, 921, 954, 1065
FspBI CTAG 1 cut(s) 350
GlaI GCGC 2 cut(s) 91, 185
GluI GCNGC 6 cut(s) 28, 182, 187, 921, 954, 1065
GsaI CCCAGC 1 cut(s) 1226
GsuI CTGGAG 1 cut(s) 801
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 5 cut(s) 30, 297, 632, 1085, 1096
HapII CCGG 3 cut(s) 31, 206, 1000
HhaI GCGC 2 cut(s) 92, 186
Hin1II CATG 5 cut(s) 17, 296, 951, 995, 1251
Hin6I GCGC 2 cut(s) 90, 184
HinP1I GCGC 2 cut(s) 90, 184
HincII GTYRAC 1 cut(s) 177
HindII GTYRAC 1 cut(s) 177
HindIII AAGCTT 1 cut(s) 387
HinfI GANTC 6 cut(s) 200, 518, 968, 1124, 1172, 1213
HpaI GTTAAC 1 cut(s) 177
HpaII CCGG 3 cut(s) 31, 206, 1000
HphI GGTGA 5 cut(s) 65, 527, 704, 725, 791
Hpy166II GTNNAC 4 cut(s) 177, 765, 1030, 1153
Hpy188III TCNNGA 6 cut(s) 197, 522, 674, 818, 932, 941
Hpy8I GTNNAC 4 cut(s) 177, 765, 1030, 1153
Hpy99I CGWCG 1 cut(s) 415
HpyAV CCTTC 5 cut(s) 153, 653, 661, 701, 1254
HpyCH4III ACNGT 3 cut(s) 227, 475, 1188
HpyCH4V TGCA 7 cut(s) 427, 536, 563, 869, 947, 962, 1067
HpyF10VI GCNNNNNNNGC 7 cut(s) 24, 533, 953, 959, 1061, 1073, 1082
HpyF3I CTNAG 5 cut(s) 93, 112, 641, 792, 1177
Hsp92II CATG 5 cut(s) 17, 296, 951, 995, 1251
HspAI GCGC 2 cut(s) 90, 184
KpnI GGTACC 2 cut(s) 37, 309
Ksp22I TGATCA 1 cut(s) 649
KspAI GTTAAC 1 cut(s) 177
Kzo9I GATC 7 cut(s) 55, 358, 649, 670, 715, 934, 1256
Lsp1109I GCAGC 3 cut(s) 193, 940, 1051
LweI GCATC 2 cut(s) 64, 934
MaeI CTAG 1 cut(s) 350
MaeIII GTNAC 2 cut(s) 254, 1132
MalI GATC 7 cut(s) 57, 360, 651, 672, 717, 936, 1258
MbiI CCGCTC 1 cut(s) 1158
MboI GATC 7 cut(s) 55, 358, 649, 670, 715, 934, 1256
MboII GAAGA 5 cut(s) 36, 112, 567, 710, 847
MfeI CAATTG 1 cut(s) 299
MflI RGATCY 3 cut(s) 670, 934, 1256
MlsI TGGCCA 1 cut(s) 297
MluCI AATT 9 cut(s) 128, 299, 319, 394, 415, 531, 555, 977, 1048
MluNI TGGCCA 1 cut(s) 297
MlyI GAGTC 3 cut(s) 194, 1133, 1222
MmeI TCCRAC 2 cut(s) 966, 1125
MnlI CCTC 7 cut(s) 90, 111, 559, 729, 814, 948, 1186
Mox20I TGGCCA 1 cut(s) 297
Mph1103I ATGCAT 1 cut(s) 949
MroXI GAANNNNTTC 2 cut(s) 1117, 1171
MscI TGGCCA 1 cut(s) 297
MseI TTAA 4 cut(s) 176, 327, 834, 912
MslI CAYNNNNRTG 1 cut(s) 432
Msp20I TGGCCA 1 cut(s) 297
MspA1I CMGCKG 2 cut(s) 374, 791
MspI CCGG 3 cut(s) 31, 206, 1000
MspR9I CCNGG 2 cut(s) 221, 753
MunI CAATTG 1 cut(s) 299
MvaI CCWGG 2 cut(s) 221, 753
MvnI CGCG 1 cut(s) 186
MwoI GCNNNNNNNGC 7 cut(s) 24, 533, 953, 959, 1061, 1073, 1082
NcoI CCATGG 1 cut(s) 13
NdeII GATC 7 cut(s) 55, 358, 649, 670, 715, 934, 1256
NlaIII CATG 5 cut(s) 17, 296, 951, 995, 1251
NlaIV GGNNCC 6 cut(s) 35, 307, 631, 672, 735, 1084
NsiI ATGCAT 1 cut(s) 949
NspI RCATGY 1 cut(s) 951
PaeR7I CTCGAG 1 cut(s) 939
PdmI GAANNNNTTC 2 cut(s) 1117, 1171
PfeI GAWTC 3 cut(s) 518, 968, 1172
PflMI CCANNNNNTGG 1 cut(s) 907
PfoI TCCNGGA 1 cut(s) 751
PkrI GCNGC 6 cut(s) 29, 183, 188, 922, 955, 1066
PleI GAGTC 3 cut(s) 194, 1132, 1221
PpsI GAGTC 3 cut(s) 194, 1132, 1221
Psp6I CCWGG 2 cut(s) 219, 751
PspEI GGTNACC 1 cut(s) 254
PspFI CCCAGC 1 cut(s) 1222
PspGI CCWGG 2 cut(s) 219, 751
PspN4I GGNNCC 6 cut(s) 35, 307, 631, 672, 735, 1084
PspPI GGNCC 3 cut(s) 80, 630, 1083
PsrI GAACNNNNNNTAC 4 cut(s) 298, 330, 748, 780
PstI CTGCAG 1 cut(s) 1069
PstNI CAGNNNCTG 1 cut(s) 231
PsuI RGATCY 3 cut(s) 670, 934, 1256
PvuII CAGCTG 2 cut(s) 374, 791
RsaI GTAC 7 cut(s) 35, 86, 167, 307, 460, 787, 1185
RsaNI GTAC 7 cut(s) 34, 85, 166, 306, 459, 786, 1184
RseI CAYNNNNRTG 1 cut(s) 432
SaqAI TTAA 4 cut(s) 176, 327, 834, 912
SatI GCNGC 6 cut(s) 28, 182, 187, 921, 954, 1065
Sau3AI GATC 7 cut(s) 55, 358, 649, 670, 715, 934, 1256
Sau96I GGNCC 3 cut(s) 80, 630, 1083
ScaI AGTACT 1 cut(s) 167
SchI GAGTC 3 cut(s) 194, 1133, 1222
ScrFI CCNGG 2 cut(s) 221, 753
SfaNI GCATC 2 cut(s) 64, 934
SfcI CTRYAG 1 cut(s) 1065
Sfr274I CTCGAG 1 cut(s) 939
SinI GGWCC 1 cut(s) 80
SlaI CTCGAG 1 cut(s) 939
SmiMI CAYNNNNRTG 1 cut(s) 432
SmlI CTYRAG 2 cut(s) 895, 939
SmoI CTYRAG 2 cut(s) 895, 939
Sse9I AATT 9 cut(s) 128, 299, 319, 394, 415, 531, 555, 977, 1048
SsiI CCGC 4 cut(s) 27, 186, 920, 1156
SspMI CTAG 1 cut(s) 350
StyD4I CCNGG 2 cut(s) 219, 751
StyI CCWWGG 3 cut(s) 13, 250, 722
TaaI ACNGT 3 cut(s) 227, 475, 1188
TaqI TCGA 5 cut(s) 521, 885, 940, 1208, 1216
TasI AATT 9 cut(s) 128, 299, 319, 394, 415, 531, 555, 977, 1048
TatI WGTACW 3 cut(s) 165, 785, 1183
TauI GCSGC 3 cut(s) 30, 189, 923
TfiI GAWTC 3 cut(s) 518, 968, 1172
Tru1I TTAA 4 cut(s) 176, 327, 834, 912
Tru9I TTAA 4 cut(s) 176, 327, 834, 912
TscAI CASTG 3 cut(s) 317, 543, 1144
TseI GCWGC 3 cut(s) 181, 953, 1064
TspDTI ATGAA 4 cut(s) 711, 1008, 1086, 1236
TspGWI ACGGA 1 cut(s) 72
TspRI CASTG 3 cut(s) 317, 543, 1144
Van91I CCANNNNNTGG 1 cut(s) 907
VpaK11BI GGWCC 1 cut(s) 80
XapI RAATTY 1 cut(s) 128
XceI RCATGY 1 cut(s) 951
XcmI CCANNNNNNNNNTGG 1 cut(s) 47
XhoI CTCGAG 1 cut(s) 939
XmnI GAANNNNTTC 2 cut(s) 1117, 1171
XspI CTAG 1 cut(s) 350
ZrmI AGTACT 1 cut(s) 167
Zsp2I ATGCAT 1 cut(s) 949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.