AT3G06770

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008733
Physical Location & Seq
Reverse (-)
2134773 .. 2137641
2869 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G06770.3

Sequence Viewer

Length: 1134 bp
ATGTCTTTCGCCGACAAAGGTGGGGCCCAGCTCTATGTTCCGCCGGGACATTGGCTCACCGGGAGTTTTAGCCTCACCAGCCACCTCACTCTCTTTCTCGAAAACGGCGCCGTTATAGTCGCTTCTCAGGATCCATCGCATTGGGAAGTGGTTGATCCTTTACCTTCATACGGAAGGGGAATTGATTTGCCTGGGAAGCGATACAAGAGTTTGATCAACGGTAACAAGCTACATGATGTTGTTGTAACAGGTGATAATGGAACTATAGATGGTCAAGGCTTGGTTTGGTGGGATCGGTTTACTTCTCATTCCTTAAAGTATAACCGTCCTCACCTCATCGAGTTTCTTTCTTCTGAAAATGTAATTGTCTCCAATCTTACGTTCTTGAATGCTCCTGCTTATTCAATCTATTCAATCTACTCTAGCCATGTATATATTCATAAGATATTGGCCCATTCTTCTCCTAAATCTCCTTATACAATCGGTATTGTACCAGATTCTTCTGATTACGTCTGCATCCAAAACTCTACCATTAACGTGGGGTATGACGCGATTTCCCTCAAAAGCGGTTGGGATGAATATGGAATTGCCTATTCTCGTCCCACTGAAAATGTTCATATAAGAAATGTGTACCTTCGAGGAGCTTCTGGTTCCTCTATCTCCTTTGGCAGTGAAATGTCTGGTGGAATATCTGATGTCGTGGTTGATAACGCTCATATACACTACTCGTTAACCGGCATTGCCTTTAGAACCACAAAAGGCAGAGGTGGTTATATCAAAGAGATCGATATTTCAAACATCGATATGTTGAGAATAGGTACTGCCATTGTTGCTAATGGTAGCTTTGGATCTCACCCTGATGATAAATATGATGTGAATGCTCTCCCACTTGTGAGTCACATCAGATTAAGCAACATTTCTGGAGAGAACATTGGCATTGCGGGGAAATTGTTTGGGATCAAAGAATCTCCATTTAGCTCAGTGACTTTGTCGAATGTTTCTTTGTCGATGAGCTCAGGTTCTTCTGTTTCTTGGCAATGCTCCTACGTTTACGGGTCCTCGGAGTCAGTCATTCCTGAACCGTGTCCCGAGCTGAAGAGAGATGCTGATGCTTACGGTAGAGCTGCAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

377

Amino Acids

40.81

Weight (kDa)

6.04

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 69 - 351 2.2e-32 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 107
AccII CGCG 1 cut(s) 551
AciI CCGC 3 cut(s) 41, 567, 941
AclWI GGATC 6 cut(s) 125, 138, 149, 300, 856, 965
AcuI CTGAAG 1 cut(s) 1115
AcyI GRCGYC 1 cut(s) 108
AfaI GTAC 3 cut(s) 492, 632, 820
AfiI CCNNNNNNNGG 1 cut(s) 170
AgsI TTSAA 4 cut(s) 388, 405, 414, 795
AjnI CCWGG 1 cut(s) 190
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
AluBI AGCT 8 cut(s) 31, 229, 644, 843, 978, 1014, 1093, 1124
AluI AGCT 8 cut(s) 31, 229, 644, 843, 978, 1014, 1093, 1124
Alw21I GWGCWC 1 cut(s) 1016
Alw26I GTCTC 1 cut(s) 373
AlwI GGATC 6 cut(s) 125, 138, 149, 300, 856, 965
Ama87I CYCGRG 1 cut(s) 1088
AoxI GGCC 2 cut(s) 24, 450
ApaI GGGCCC 1 cut(s) 28
ApeKI GCWGC 1 cut(s) 1124
Asp700I GAANNNNTTC 1 cut(s) 612
AspLEI GCGC 1 cut(s) 110
AspS9I GGNCC 4 cut(s) 24, 25, 451, 1056
AsuC2I CCSGG 2 cut(s) 45, 61
AsuHPI GGTGA 5 cut(s) 49, 67, 263, 323, 845
AvaI CYCGRG 1 cut(s) 1088
AvaII GGWCC 1 cut(s) 1056
BaeGI GKGCMC 1 cut(s) 28
BaeI ACNNNNGTAYC 2 cut(s) 193, 226
BamHI GGATCC 1 cut(s) 130
BanI GGYRCC 1 cut(s) 107
BanII GRGCYC 2 cut(s) 28, 1016
Bbv12I GWGCWC 1 cut(s) 1016
BbvI GCAGC 1 cut(s) 1111
BccI CCATC 2 cut(s) 142, 263
BceAI ACGGC 2 cut(s) 95, 121
BciT130I CCWGG 1 cut(s) 192
BclI TGATCA 1 cut(s) 213
BcnI CCSGG 2 cut(s) 45, 61
BcoDI GTCTC 1 cut(s) 373
BfaI CTAG 2 cut(s) 423, 1132
BfmI CTRYAG 2 cut(s) 264, 1125
BfoI RGCGCY 1 cut(s) 111
BisI GCNGC 1 cut(s) 1125
BlsI GCNGC 1 cut(s) 1126
Bme1390I CCNGG 3 cut(s) 45, 61, 192
Bme18I GGWCC 1 cut(s) 1056
BmeT110I CYCGRG 1 cut(s) 1088
BmgT120I GGNCC 4 cut(s) 24, 25, 451, 1056
BmiI GGNNCC 6 cut(s) 25, 26, 109, 132, 652, 1057
BmrFI CCNGG 3 cut(s) 45, 61, 192
BmsI GCATC 3 cut(s) 525, 1093, 1099
BpmI CTGGAG 1 cut(s) 942
Bpu10I CCTNAGC 1 cut(s) 1015
BpuMI CCSGG 2 cut(s) 45, 61
Bsa29I ATCGAT 2 cut(s) 786, 801
BsaHI GRCGYC 1 cut(s) 108
BsaJI CCNNGG 2 cut(s) 191, 1059
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse118I RCCGGY 1 cut(s) 734
Bse3DI GCAATG 3 cut(s) 738, 936, 1043
BseBI CCWGG 1 cut(s) 192
BseCI ATCGAT 2 cut(s) 786, 801
BseDI CCNNGG 2 cut(s) 191, 1059
BseGI GGATG 2 cut(s) 516, 580
BseLI CCNNNNNNNGG 1 cut(s) 170
BseMI GCAATG 3 cut(s) 738, 936, 1043
BseMII CTCAG 3 cut(s) 140, 993, 1029
BseRI GAGGAG 1 cut(s) 654
BseSI GKGCMC 1 cut(s) 28
BseXI GCAGC 1 cut(s) 1111
BseYI CCCAGC 1 cut(s) 27
Bsh1236I CGCG 1 cut(s) 551
BshFI GGCC 2 cut(s) 26, 452
BshNI GGYRCC 1 cut(s) 107
BshVI ATCGAT 2 cut(s) 786, 801
BsiHKAI GWGCWC 1 cut(s) 1016
BsiHKCI CYCGRG 1 cut(s) 1088
BsiSI CCGG 3 cut(s) 44, 60, 735
BslFI GGGAC 3 cut(s) 60, 585, 1071
BslI CCNNNNNNNGG 1 cut(s) 170
BsmAI GTCTC 1 cut(s) 373
BsmFI GGGAC 3 cut(s) 60, 585, 1071
BsmI GAATGC 2 cut(s) 394, 883
BsnI GGCC 2 cut(s) 26, 452
BsoBI CYCGRG 1 cut(s) 1088
Bsp120I GGGCCC 1 cut(s) 24
Bsp1286I GDGCHC 2 cut(s) 28, 1016
Bsp143I GATC 7 cut(s) 130, 154, 213, 292, 783, 848, 957
BspACI CCGC 3 cut(s) 41, 567, 941
BspANI GGCC 2 cut(s) 26, 452
BspCNI CTCAG 3 cut(s) 139, 992, 1028
BspDI ATCGAT 2 cut(s) 786, 801
BspFNI CGCG 1 cut(s) 551
BspLI GGNNCC 6 cut(s) 25, 26, 109, 132, 652, 1057
BspMAI CTGCAG 1 cut(s) 1129
BspPI GGATC 6 cut(s) 125, 138, 149, 300, 856, 965
BspT107I GGYRCC 1 cut(s) 107
BsrDI GCAATG 3 cut(s) 738, 936, 1043
BsrFI RCCGGY 1 cut(s) 734
BssAI RCCGGY 1 cut(s) 734
BssECI CCNNGG 2 cut(s) 191, 1059
BssMI GATC 7 cut(s) 130, 154, 213, 292, 783, 848, 957
BssNI GRCGYC 1 cut(s) 108
Bst2UI CCWGG 1 cut(s) 192
Bst4CI ACNGT 4 cut(s) 221, 326, 1083, 1118
Bst6I CTCTTC 1 cut(s) 1091
BstACI GRCGYC 1 cut(s) 108
BstDEI CTNAG 3 cut(s) 126, 979, 1015
BstF5I GGATG 2 cut(s) 516, 580
BstFNI CGCG 1 cut(s) 551
BstH2I RGCGCY 1 cut(s) 111
BstHHI GCGC 1 cut(s) 110
BstKTI GATC 7 cut(s) 133, 157, 216, 295, 786, 851, 960
BstMAI GTCTC 1 cut(s) 373
BstMBI GATC 7 cut(s) 130, 154, 213, 292, 783, 848, 957
BstMWI GCNNNNNNNGC 3 cut(s) 78, 196, 830
BstNI CCWGG 1 cut(s) 192
BstSCI CCNGG 3 cut(s) 43, 59, 190
BstSFI CTRYAG 2 cut(s) 264, 1125
BstSLI GKGCMC 1 cut(s) 28
BstUI CGCG 1 cut(s) 551
BstV1I GCAGC 1 cut(s) 1111
BstX2I RGATCY 2 cut(s) 130, 848
BstXI CCANNNNNNTGG 2 cut(s) 141, 538
BstYI RGATCY 2 cut(s) 130, 848
Bsu15I ATCGAT 2 cut(s) 786, 801
BsuRI GGCC 2 cut(s) 26, 452
BsuTUI ATCGAT 2 cut(s) 786, 801
BtgZI GCGATG 1 cut(s) 120
BtsCI GGATG 2 cut(s) 516, 580
BtsI GCAGTG 1 cut(s) 676
BtsIMutI CAGTG 3 cut(s) 603, 676, 987
CfoI GCGC 1 cut(s) 110
Cfr10I RCCGGY 1 cut(s) 734
Cfr13I GGNCC 4 cut(s) 24, 25, 451, 1056
ClaI ATCGAT 2 cut(s) 786, 801
CseI GACGC 1 cut(s) 557
Csp6I GTAC 3 cut(s) 491, 631, 819
CviAII CATG 2 cut(s) 233, 428
CviQI GTAC 3 cut(s) 491, 631, 819
DdeI CTNAG 3 cut(s) 126, 979, 1015
DinI GGCGCC 1 cut(s) 109
DpnI GATC 7 cut(s) 132, 156, 215, 294, 785, 850, 959
DpnII GATC 7 cut(s) 130, 154, 213, 292, 783, 848, 957
Eam1104I CTCTTC 1 cut(s) 1091
EarI CTCTTC 1 cut(s) 1091
EciI GGCGGA 1 cut(s) 30
Ecl136II GAGCTC 1 cut(s) 1014
Eco24I GRGCYC 2 cut(s) 28, 1016
Eco47I GGWCC 1 cut(s) 1056
Eco53kI GAGCTC 1 cut(s) 1014
Eco57I CTGAAG 1 cut(s) 1115
Eco88I CYCGRG 1 cut(s) 1088
EcoICRI GAGCTC 1 cut(s) 1014
EcoO109I RGGNCCY 2 cut(s) 24, 1056
EcoRII CCWGG 1 cut(s) 190
EcoT38I GRGCYC 2 cut(s) 28, 1016
EgeI GGCGCC 1 cut(s) 109
EheI GGCGCC 1 cut(s) 109
FaeI CATG 2 cut(s) 236, 431
FaqI GGGAC 3 cut(s) 60, 585, 1071
FatI CATG 2 cut(s) 232, 427
FauI CCCGC 1 cut(s) 934
FbaI TGATCA 1 cut(s) 213
Fnu4HI GCNGC 1 cut(s) 1125
FokI GGATG 2 cut(s) 503, 587
FriOI GRGCYC 2 cut(s) 28, 1016
Fsp4HI GCNGC 1 cut(s) 1125
FspBI CTAG 2 cut(s) 423, 1132
GlaI GCGC 1 cut(s) 109
GluI GCNGC 1 cut(s) 1125
GsaI CCCAGC 1 cut(s) 31
GsuI CTGGAG 1 cut(s) 942
HaeII RGCGCY 1 cut(s) 111
HaeIII GGCC 2 cut(s) 26, 452
HapII CCGG 3 cut(s) 44, 60, 735
HgaI GACGC 1 cut(s) 557
HhaI GCGC 1 cut(s) 110
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 2 cut(s) 236, 431
Hin6I GCGC 1 cut(s) 108
HinP1I GCGC 1 cut(s) 108
HincII GTYRAC 1 cut(s) 732
HindII GTYRAC 1 cut(s) 732
HinfI GANTC 4 cut(s) 497, 895, 965, 1064
HpaI GTTAAC 1 cut(s) 732
HpaII CCGG 3 cut(s) 44, 60, 735
HphI GGTGA 5 cut(s) 49, 67, 263, 323, 845
Hpy166II GTNNAC 4 cut(s) 300, 631, 732, 1051
Hpy188I TCNGA 5 cut(s) 355, 505, 694, 905, 1063
Hpy188III TCNNGA 6 cut(s) 98, 128, 385, 921, 1076, 1088
Hpy8I GTNNAC 4 cut(s) 300, 631, 732, 1051
HpyAV CCTTC 3 cut(s) 168, 174, 644
HpyCH4III ACNGT 4 cut(s) 221, 326, 1083, 1118
HpyCH4IV ACGT 4 cut(s) 380, 510, 537, 1047
HpyCH4V TGCA 2 cut(s) 516, 1127
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 196, 830
HpyF3I CTNAG 3 cut(s) 126, 979, 1015
HpySE526I ACGT 4 cut(s) 380, 510, 537, 1047
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 2 cut(s) 236, 431
HspAI GCGC 1 cut(s) 108
KasI GGCGCC 1 cut(s) 107
Ksp22I TGATCA 1 cut(s) 213
KspAI GTTAAC 1 cut(s) 732
Kzo9I GATC 7 cut(s) 130, 154, 213, 292, 783, 848, 957
LmnI GCTCC 3 cut(s) 397, 641, 1046
Lsp1109I GCAGC 1 cut(s) 1111
LweI GCATC 3 cut(s) 525, 1093, 1099
MaeI CTAG 2 cut(s) 423, 1132
MaeII ACGT 4 cut(s) 380, 510, 537, 1047
MaeIII GTNAC 4 cut(s) 221, 244, 896, 982
MalI GATC 7 cut(s) 132, 156, 215, 294, 785, 850, 959
MboI GATC 7 cut(s) 130, 154, 213, 292, 783, 848, 957
MboII GAAGA 5 cut(s) 342, 450, 492, 1014, 1108
MflI RGATCY 2 cut(s) 130, 848
MhlI GDGCHC 2 cut(s) 28, 1016
MluCI AATT 4 cut(s) 180, 363, 585, 947
Mly113I GGCGCC 1 cut(s) 108
MlyI GAGTC 2 cut(s) 904, 1073
MnlI CCTC 9 cut(s) 83, 95, 339, 344, 569, 632, 664, 758, 1069
MroXI GAANNNNTTC 1 cut(s) 612
MseI TTAA 4 cut(s) 314, 534, 731, 908
MslI CAYNNNNRTG 3 cut(s) 536, 803, 858
MspI CCGG 3 cut(s) 44, 60, 735
MspR9I CCNGG 3 cut(s) 45, 61, 192
Mva1269I GAATGC 2 cut(s) 394, 883
MvaI CCWGG 1 cut(s) 192
MvnI CGCG 1 cut(s) 551
MwoI GCNNNNNNNGC 3 cut(s) 78, 196, 830
NarI GGCGCC 1 cut(s) 108
NciI CCSGG 2 cut(s) 45, 61
NdeII GATC 7 cut(s) 130, 154, 213, 292, 783, 848, 957
NlaIII CATG 2 cut(s) 236, 431
NlaIV GGNNCC 6 cut(s) 25, 26, 109, 132, 652, 1057
NmuCI GTSAC 2 cut(s) 896, 982
PctI GAATGC 2 cut(s) 394, 883
PdmI GAANNNNTTC 1 cut(s) 612
PfeI GAWTC 2 cut(s) 497, 965
PflFI GACNNNGTC 1 cut(s) 988
PkrI GCNGC 1 cut(s) 1126
PleI GAGTC 2 cut(s) 903, 1072
PluTI GGCGCC 1 cut(s) 111
PpsI GAGTC 2 cut(s) 903, 1072
PpuMI RGGWCCY 1 cut(s) 1056
Psp124BI GAGCTC 1 cut(s) 1016
Psp5II RGGWCCY 1 cut(s) 1056
Psp6I CCWGG 1 cut(s) 190
PspFI CCCAGC 1 cut(s) 27
PspGI CCWGG 1 cut(s) 190
PspN4I GGNNCC 6 cut(s) 25, 26, 109, 132, 652, 1057
PspOMI GGGCCC 1 cut(s) 24
PspPI GGNCC 4 cut(s) 24, 25, 451, 1056
PspPPI RGGWCCY 1 cut(s) 1056
PstI CTGCAG 1 cut(s) 1129
PsuI RGATCY 2 cut(s) 130, 848
PsyI GACNNNGTC 1 cut(s) 988
RsaI GTAC 3 cut(s) 492, 632, 820
RsaNI GTAC 3 cut(s) 491, 631, 819
RseI CAYNNNNRTG 3 cut(s) 536, 803, 858
SacI GAGCTC 1 cut(s) 1016
SaqAI TTAA 4 cut(s) 314, 534, 731, 908
SatI GCNGC 1 cut(s) 1125
Sau3AI GATC 7 cut(s) 130, 154, 213, 292, 783, 848, 957
Sau96I GGNCC 4 cut(s) 24, 25, 451, 1056
SchI GAGTC 2 cut(s) 904, 1073
ScrFI CCNGG 3 cut(s) 45, 61, 192
SduI GDGCHC 2 cut(s) 28, 1016
SfaNI GCATC 3 cut(s) 525, 1093, 1099
SfcI CTRYAG 2 cut(s) 264, 1125
SfoI GGCGCC 1 cut(s) 109
SinI GGWCC 1 cut(s) 1056
SmiMI CAYNNNNRTG 3 cut(s) 536, 803, 858
Sse9I AATT 4 cut(s) 180, 363, 585, 947
SsiI CCGC 3 cut(s) 41, 567, 941
SspDI GGCGCC 1 cut(s) 107
SspMI CTAG 2 cut(s) 423, 1132
SstI GAGCTC 1 cut(s) 1016
StyD4I CCNGG 3 cut(s) 43, 59, 190
TaaI ACNGT 4 cut(s) 221, 326, 1083, 1118
TaiI ACGT 4 cut(s) 383, 513, 540, 1050
TaqI TCGA 7 cut(s) 99, 339, 637, 786, 801, 992, 1007
TasI AATT 4 cut(s) 180, 363, 585, 947
TfiI GAWTC 2 cut(s) 497, 965
Tru1I TTAA 4 cut(s) 314, 534, 731, 908
Tru9I TTAA 4 cut(s) 314, 534, 731, 908
TscAI CASTG 3 cut(s) 610, 676, 987
TseFI GTSAC 2 cut(s) 896, 982
TseI GCWGC 1 cut(s) 1124
Tsp45I GTSAC 2 cut(s) 896, 982
TspDTI ATGAA 4 cut(s) 156, 428, 591, 605
TspGWI ACGGA 1 cut(s) 186
TspRI CASTG 3 cut(s) 610, 676, 987
Tth111I GACNNNGTC 1 cut(s) 988
VpaK11BI GGWCC 1 cut(s) 1056
XmnI GAANNNNTTC 1 cut(s) 612
XspI CTAG 2 cut(s) 423, 1132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.