Rh4DG250200

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0008733
Physical Location & Seq
Forward (+)
46334548 .. 46349671
15124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG250200.1

Sequence Viewer

Length: 1461 bp
ATGCCATGTGGTCTTGACCAAGAAATTATGTTATATAATTACACAGACAAAAAATCTGAATCTCAGGCTTCTGGTTCTGTGGCTTTCTTGTTCTTGCATTTTCAGGTGGTATTGCTCTTGCTGCTGAGTTTGGGCGATGCTATTGGTGGAGAAGACGAGGAGAGGCCTTGTGATTCTAAACTGCAATTAAAACCAAGGCCGCATAGTGTTTCCATTTTGGAGTTTGGTGCTGTTGGAGATGGCAAGACATTGAACACTCTTGCTTTCCAGAATGCCATTTTCTATCTCAAGTCTTTTGCTGACAAGGGCGGTGCACAGCTTTACGTGCCGCCCGGAAGATGGCTTACCGGAAGTTTCAACCTTACGAGCCATCTCACACTGTTTTTGGAAAAGGGTGCTGTCATTCTTGGATCTCAGGATCCATCTCATTGGGGTATTCTTGAACCCTTGCCCTCGTACGGTCGAGGGATTGAACTCCCAGGAGGGAGATATTGTAGCTTGATAAATGGATATATGTTACAGGATGTGGTCATAACAGGTGATAATGGAACCATCAACGGTCAAGGATCAGTGTGGTGGGATTGGTTTAACTCTCATACTTTAAACCATAGCCGTCCTCATCTTATAGAGTTAGTTTCGTCTAAAGATGTTGTTGTTTCAAACCTCACATTTGTGAATTCTCCTGCATACAACATTCATCCAGTCTATTGCAGTAATGTACATATTCACAACATCTCAGTCTCTGCTCCCCCGGAGTCCCCTTATACAGTCGGTATAGTCCCAGATTCTTCTGACAATGTGTGCATACAAGATTGCAGCATTGGCATGGGTTATGATGCCATTGTCCTCAAGAGTGGTTGGGACGAGTATGGCATTGCCTATGGAAGACCAACCACAAATGTACACATCAGACGGGTCCAGCTACAATCGTCTTCTGGTTCTTCTCTTGCCTTTGGTAGTGAGATGTCCGGTGGCATTTCTAATGTGCTTGTGGAGCAGGTCCACCTATACAACTCACTTAGTGGCATTCAGTTTAGAACAACAAAGGGTAGAGGTGGTTATATCAAAGATATCGTAATTTCAGATGTCAAAATGGAAAACATCGACGTGGCATTTGGTGCGTCTGGTCAGTTTGGTTCCCATCCGGATGACAACTTCGATCCTAATGCCCTTCCAGTATTGGATCACATCAACTTACAGGATGTGATTGGTACCAACATCACTATTGCTGGAAAATTCTCTGGGATCAAGGAATCCCCCTTCACTTCCTTTTGTCTGTCCAATATCTCCTTGTCAATCAATTCTGGTTCTTCTACCAATTCTTGGGTATGTTCAAATGTTTCTGGCTCTTCTGAGTCTGTCTTCCCCGAACCATGTTCTGCCCTTAAGTCATCGTATTCAAATTCTTCCTCAGCCTGCTTTTCAATACAGACCTTGAATGGAACAGCCGTGGCATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

486

Amino Acids

52.29

Weight (kDa)

5.23

Isoelectric Point (pI)

46.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 168 - 448 1.6e-27 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 988
Acc65I GGTACC 1 cut(s) 1211
AccB1I GGYRCC 1 cut(s) 1211
AccB7I CCANNNNNTGG 1 cut(s) 1323
AccIII TCCGGA 1 cut(s) 1144
AciI CCGC 3 cut(s) 200, 309, 329
AclWI GGATC 7 cut(s) 413, 418, 426, 574, 1154, 1191, 1253
AcsI RAATTY 3 cut(s) 676, 1235, 1402
AdeI CACNNNGTG 1 cut(s) 1022
AfaI GTAC 4 cut(s) 458, 720, 903, 1213
AfiI CCNNNNNNNGG 3 cut(s) 339, 458, 1323
AflII CTTAAG 1 cut(s) 1385
AgsI TTSAA 9 cut(s) 253, 358, 443, 473, 660, 1335, 1401, 1425, 1438
AjiI CACGTC 1 cut(s) 1108
AjnI CCWGG 1 cut(s) 478
AleI CACNNNNGTG 1 cut(s) 671
AluBI AGCT 3 cut(s) 319, 498, 922
AluI AGCT 3 cut(s) 319, 498, 922
Alw21I GWGCWC 1 cut(s) 316
Alw26I GTCTC 1 cut(s) 745
Alw44I GTGCAC 1 cut(s) 312
AlwI GGATC 7 cut(s) 413, 418, 426, 574, 1154, 1191, 1253
AlwNI CAGNNNCTG 1 cut(s) 743
Aor13HI TCCGGA 1 cut(s) 1144
AoxI GGCC 2 cut(s) 164, 197
ApaLI GTGCAC 1 cut(s) 312
ApeKI GCWGC 2 cut(s) 121, 816
ApoI RAATTY 3 cut(s) 676, 1235, 1402
ArsI GACNNNNNNTTYG 2 cut(s) 1097, 1129
Asp718I GGTACC 1 cut(s) 1211
AspS9I GGNCC 2 cut(s) 916, 1000
AsuC2I CCSGG 2 cut(s) 333, 752
AsuHPI GGTGA 1 cut(s) 551
AvaII GGWCC 2 cut(s) 916, 1000
BaeGI GKGCMC 1 cut(s) 316
BamHI GGATCC 1 cut(s) 418
BanI GGYRCC 1 cut(s) 1211
BarI GAAGNNNNNNTAC 2 cut(s) 328, 360
BbsI GAAGAC 4 cut(s) 159, 892, 924, 1354
Bbv12I GWGCWC 1 cut(s) 316
BbvCI CCTCAGC 1 cut(s) 1411
BbvI GCAGC 2 cut(s) 108, 828
BccI CCATC 6 cut(s) 233, 333, 378, 430, 560, 1149
BceAI ACGGC 2 cut(s) 597, 1433
BcgI CGANNNNNNTGC 2 cut(s) 1148, 1182
BciT130I CCWGG 1 cut(s) 480
BcnI CCSGG 2 cut(s) 333, 752
BcoDI GTCTC 1 cut(s) 745
BfrI CTTAAG 1 cut(s) 1385
BfuAI ACCTGC 1 cut(s) 988
BisI GCNGC 4 cut(s) 122, 200, 329, 817
BlsI GCNGC 4 cut(s) 123, 201, 330, 818
Bme1390I CCNGG 3 cut(s) 333, 480, 752
Bme18I GGWCC 2 cut(s) 916, 1000
BmgBI CACGTC 1 cut(s) 1108
BmgT120I GGNCC 2 cut(s) 916, 1000
BmiI GGNNCC 5 cut(s) 420, 550, 917, 1138, 1213
BmrFI CCNGG 3 cut(s) 333, 480, 752
BmsI GCATC 2 cut(s) 127, 826
BpiI GAAGAC 4 cut(s) 159, 892, 924, 1354
Bpu10I CCTNAGC 1 cut(s) 1411
BpuEI CTTGAG 2 cut(s) 272, 833
BpuMI CCSGG 2 cut(s) 333, 752
BsaAI YACGTR 1 cut(s) 325
BsaJI CCNNGG 4 cut(s) 194, 478, 750, 1449
BsaWI WCCGGW 3 cut(s) 347, 968, 1144
BsaXI ACNNNNNCTCC 2 cut(s) 478, 508
Bsc4I CCNNNNNNNGG 3 cut(s) 339, 458, 1323
Bse1I ACTGG 2 cut(s) 701, 1175
Bse3DI GCAATG 1 cut(s) 873
BseAI TCCGGA 1 cut(s) 1144
BseBI CCWGG 1 cut(s) 480
BseDI CCNNGG 4 cut(s) 194, 478, 750, 1449
BseGI GGATG 5 cut(s) 529, 697, 1141, 1153, 1207
BseLI CCNNNNNNNGG 3 cut(s) 339, 458, 1323
BseMI GCAATG 1 cut(s) 873
BseMII CTCAG 6 cut(s) 77, 116, 428, 750, 1344, 1425
BseNI ACTGG 2 cut(s) 701, 1175
BseRI GAGGAG 1 cut(s) 173
BseSI GKGCMC 1 cut(s) 316
BseXI GCAGC 2 cut(s) 108, 828
Bsh1285I CGRYCG 1 cut(s) 463
BshFI GGCC 2 cut(s) 166, 199
BshNI GGYRCC 1 cut(s) 1211
BsiEI CGRYCG 1 cut(s) 463
BsiHKAI GWGCWC 1 cut(s) 316
BsiSI CCGG 5 cut(s) 333, 348, 752, 969, 1145
BsiWI CGTACG 1 cut(s) 456
BslFI GGGAC 3 cut(s) 742, 764, 875
BslI CCNNNNNNNGG 3 cut(s) 339, 458, 1323
BsmAI GTCTC 1 cut(s) 745
BsmFI GGGAC 3 cut(s) 742, 764, 875
BsmI GAATGC 2 cut(s) 277, 1026
BsnI GGCC 2 cut(s) 166, 199
Bsp1286I GDGCHC 1 cut(s) 316
Bsp13I TCCGGA 1 cut(s) 1144
Bsp1407I TGTACA 2 cut(s) 718, 901
Bsp143I GATC 6 cut(s) 410, 418, 566, 1159, 1183, 1245
BspACI CCGC 3 cut(s) 200, 309, 329
BspANI GGCC 2 cut(s) 166, 199
BspCNI CTCAG 6 cut(s) 76, 117, 427, 749, 1345, 1424
BspEI TCCGGA 1 cut(s) 1144
BspLI GGNNCC 5 cut(s) 420, 550, 917, 1138, 1213
BspMI ACCTGC 1 cut(s) 988
BspPI GGATC 7 cut(s) 413, 418, 426, 574, 1154, 1191, 1253
BspQI GCTCTTC 1 cut(s) 1354
BspT107I GGYRCC 1 cut(s) 1211
BspTI CTTAAG 1 cut(s) 1385
BsrDI GCAATG 1 cut(s) 873
BsrGI TGTACA 2 cut(s) 718, 901
BsrI ACTGG 2 cut(s) 701, 1175
BssECI CCNNGG 4 cut(s) 194, 478, 750, 1449
BssMI GATC 6 cut(s) 410, 418, 566, 1159, 1183, 1245
BssT1I CCWWGG 1 cut(s) 194
Bst2UI CCWGG 1 cut(s) 480
Bst4CI ACNGT 4 cut(s) 381, 461, 560, 769
Bst6I CTCTTC 1 cut(s) 1354
BstAFI CTTAAG 1 cut(s) 1385
BstAPI GCANNNNNTGC 1 cut(s) 1118
BstAUI TGTACA 2 cut(s) 718, 901
BstBAI YACGTR 1 cut(s) 325
BstC8I GCNNGC 1 cut(s) 1417
BstDEI CTNAG 7 cut(s) 63, 125, 414, 736, 1019, 1353, 1411
BstDSI CCRYGG 1 cut(s) 1449
BstF5I GGATG 5 cut(s) 529, 697, 1141, 1153, 1207
BstKTI GATC 6 cut(s) 413, 421, 569, 1162, 1186, 1248
BstMAI GTCTC 1 cut(s) 745
BstMBI GATC 6 cut(s) 410, 418, 566, 1159, 1183, 1245
BstMCI CGRYCG 1 cut(s) 463
BstMWI GCNNNNNNNGC 5 cut(s) 121, 325, 822, 994, 1118
BstNI CCWGG 1 cut(s) 480
BstSCI CCNGG 3 cut(s) 331, 478, 750
BstSLI GKGCMC 1 cut(s) 316
BstV1I GCAGC 2 cut(s) 108, 828
BstV2I GAAGAC 4 cut(s) 159, 892, 924, 1354
BstX2I RGATCY 2 cut(s) 410, 418
BstXI CCANNNNNNTGG 1 cut(s) 429
BstYI RGATCY 2 cut(s) 410, 418
BsuRI GGCC 2 cut(s) 166, 199
BtgI CCRYGG 1 cut(s) 1449
BtgZI GCGATG 1 cut(s) 150
BtrI CACGTC 1 cut(s) 1108
BtsCI GGATG 5 cut(s) 529, 697, 1141, 1153, 1207
BtsIMutI CAGTG 2 cut(s) 377, 576
BveI ACCTGC 1 cut(s) 988
Cac8I GCNNGC 1 cut(s) 1417
CaiI CAGNNNCTG 1 cut(s) 743
Cfr13I GGNCC 2 cut(s) 916, 1000
CseI GACGC 1 cut(s) 1110
Csp6I GTAC 4 cut(s) 457, 719, 902, 1212
CviAII CATG 3 cut(s) 6, 826, 1374
CviQI GTAC 4 cut(s) 457, 719, 902, 1212
DdeI CTNAG 7 cut(s) 63, 125, 414, 736, 1019, 1353, 1411
DpnI GATC 6 cut(s) 412, 420, 568, 1161, 1185, 1247
DpnII GATC 6 cut(s) 410, 418, 566, 1159, 1183, 1245
DraI TTTAAA 1 cut(s) 603
DraIII CACNNNGTG 1 cut(s) 1022
Eam1104I CTCTTC 1 cut(s) 1354
EarI CTCTTC 1 cut(s) 1354
Eco130I CCWWGG 1 cut(s) 194
Eco147I AGGCCT 1 cut(s) 166
Eco32I GATATC 1 cut(s) 1072
Eco47I GGWCC 2 cut(s) 916, 1000
EcoRI GAATTC 1 cut(s) 676
EcoRII CCWGG 1 cut(s) 478
EcoRV GATATC 1 cut(s) 1072
EcoT14I CCWWGG 1 cut(s) 194
ErhI CCWWGG 1 cut(s) 194
FaeI CATG 3 cut(s) 9, 829, 1377
FaqI GGGAC 3 cut(s) 742, 764, 875
FatI CATG 3 cut(s) 5, 825, 1373
Fnu4HI GCNGC 4 cut(s) 122, 200, 329, 817
FokI GGATG 5 cut(s) 536, 684, 1128, 1160, 1214
Fsp4HI GCNGC 4 cut(s) 122, 200, 329, 817
GluI GCNGC 4 cut(s) 122, 200, 329, 817
HaeIII GGCC 2 cut(s) 166, 199
HapII CCGG 5 cut(s) 333, 348, 752, 969, 1145
HgaI GACGC 1 cut(s) 1110
Hin1II CATG 3 cut(s) 9, 829, 1377
HinfI GANTC 6 cut(s) 59, 173, 755, 785, 1253, 1355
HpaII CCGG 5 cut(s) 333, 348, 752, 969, 1145
HphI GGTGA 1 cut(s) 551
Hpy166II GTNNAC 3 cut(s) 314, 904, 1003
Hpy188I TCNGA 5 cut(s) 58, 793, 911, 1084, 1354
Hpy188III TCNNGA 6 cut(s) 14, 268, 416, 440, 850, 1145
Hpy8I GTNNAC 3 cut(s) 314, 904, 1003
Hpy99I CGWCG 1 cut(s) 1109
HpyAV CCTTC 2 cut(s) 1181, 1270
HpyCH4III ACNGT 4 cut(s) 381, 461, 560, 769
HpyCH4IV ACGT 2 cut(s) 324, 1107
HpyCH4V TGCA 7 cut(s) 97, 184, 314, 686, 711, 804, 816
HpyF10VI GCNNNNNNNGC 5 cut(s) 121, 325, 822, 994, 1118
HpyF3I CTNAG 7 cut(s) 63, 125, 414, 736, 1019, 1353, 1411
HpySE526I ACGT 2 cut(s) 324, 1107
Hsp92II CATG 3 cut(s) 9, 829, 1377
Kpn2I TCCGGA 1 cut(s) 1144
KpnI GGTACC 1 cut(s) 1215
Kzo9I GATC 6 cut(s) 410, 418, 566, 1159, 1183, 1245
LguI GCTCTTC 1 cut(s) 1354
LmnI GCTCC 2 cut(s) 751, 994
Lsp1109I GCAGC 2 cut(s) 108, 828
LweI GCATC 2 cut(s) 127, 826
MaeII ACGT 2 cut(s) 324, 1107
MaeIII GTNAC 1 cut(s) 516
MalI GATC 6 cut(s) 412, 420, 568, 1161, 1185, 1247
MboI GATC 6 cut(s) 410, 418, 566, 1159, 1183, 1245
MflI RGATCY 2 cut(s) 410, 418
MhlI GDGCHC 1 cut(s) 316
MluCI AATT 9 cut(s) 24, 37, 185, 676, 1077, 1235, 1300, 1318, 1402
MlyI GAGTC 2 cut(s) 764, 1364
MmeI TCCRAC 1 cut(s) 214
MroI TCCGGA 1 cut(s) 1144
MseI TTAA 4 cut(s) 188, 588, 602, 1386
MslI CAYNNNNRTG 4 cut(s) 671, 824, 1106, 1146
MspCI CTTAAG 1 cut(s) 1385
MspI CCGG 5 cut(s) 333, 348, 752, 969, 1145
MspR9I CCNGG 3 cut(s) 333, 480, 752
Mva1269I GAATGC 2 cut(s) 277, 1026
MvaI CCWGG 1 cut(s) 480
MwoI GCNNNNNNNGC 5 cut(s) 121, 325, 822, 994, 1118
NciI CCSGG 2 cut(s) 333, 752
NdeII GATC 6 cut(s) 410, 418, 566, 1159, 1183, 1245
NlaIII CATG 3 cut(s) 9, 829, 1377
NlaIV GGNNCC 5 cut(s) 420, 550, 917, 1138, 1213
OliI CACNNNNGTG 1 cut(s) 671
PceI AGGCCT 1 cut(s) 166
PciSI GCTCTTC 1 cut(s) 1354
PctI GAATGC 2 cut(s) 277, 1026
PfeI GAWTC 4 cut(s) 59, 173, 785, 1253
Pfl23II CGTACG 1 cut(s) 456
PflMI CCANNNNNTGG 1 cut(s) 1323
PkrI GCNGC 4 cut(s) 123, 201, 330, 818
PleI GAGTC 2 cut(s) 763, 1363
PpsI GAGTC 2 cut(s) 763, 1363
Ppu21I YACGTR 1 cut(s) 325
Psp6I CCWGG 1 cut(s) 478
PspGI CCWGG 1 cut(s) 478
PspLI CGTACG 1 cut(s) 456
PspN4I GGNNCC 5 cut(s) 420, 550, 917, 1138, 1213
PspPI GGNCC 2 cut(s) 916, 1000
PstNI CAGNNNCTG 1 cut(s) 743
PsuI RGATCY 2 cut(s) 410, 418
RsaI GTAC 4 cut(s) 458, 720, 903, 1213
RsaNI GTAC 4 cut(s) 457, 719, 902, 1212
RseI CAYNNNNRTG 4 cut(s) 671, 824, 1106, 1146
SapI GCTCTTC 1 cut(s) 1354
SaqAI TTAA 4 cut(s) 188, 588, 602, 1386
SatI GCNGC 4 cut(s) 122, 200, 329, 817
Sau3AI GATC 6 cut(s) 410, 418, 566, 1159, 1183, 1245
Sau96I GGNCC 2 cut(s) 916, 1000
SchI GAGTC 2 cut(s) 764, 1364
ScrFI CCNGG 3 cut(s) 333, 480, 752
SduI GDGCHC 1 cut(s) 316
SfaNI GCATC 2 cut(s) 127, 826
SinI GGWCC 2 cut(s) 916, 1000
SmiMI CAYNNNNRTG 4 cut(s) 671, 824, 1106, 1146
SmlI CTYRAG 3 cut(s) 287, 848, 1385
SmoI CTYRAG 3 cut(s) 287, 848, 1385
Sse9I AATT 9 cut(s) 24, 37, 185, 676, 1077, 1235, 1300, 1318, 1402
SseBI AGGCCT 1 cut(s) 166
SsiI CCGC 3 cut(s) 200, 309, 329
StuI AGGCCT 1 cut(s) 166
StyD4I CCNGG 3 cut(s) 331, 478, 750
StyI CCWWGG 1 cut(s) 194
TaaI ACNGT 4 cut(s) 381, 461, 560, 769
TaiI ACGT 2 cut(s) 327, 1110
TaqI TCGA 3 cut(s) 463, 1104, 1158
TasI AATT 9 cut(s) 24, 37, 185, 676, 1077, 1235, 1300, 1318, 1402
TatI WGTACW 2 cut(s) 718, 901
TauI GCSGC 2 cut(s) 202, 331
TfiI GAWTC 4 cut(s) 59, 173, 785, 1253
Tru1I TTAA 4 cut(s) 188, 588, 602, 1386
Tru9I TTAA 4 cut(s) 188, 588, 602, 1386
TscAI CASTG 2 cut(s) 384, 576
TseI GCWGC 2 cut(s) 121, 816
TspDTI ATGAA 1 cut(s) 686
TspRI CASTG 2 cut(s) 384, 576
Van91I CCANNNNNTGG 1 cut(s) 1323
Vha464I CTTAAG 1 cut(s) 1385
VneI GTGCAC 1 cut(s) 312
VpaK11BI GGWCC 2 cut(s) 916, 1000
XapI RAATTY 3 cut(s) 676, 1235, 1402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.