Rh4AG250400

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0008733
Physical Location & Seq
Forward (+)
56250908 .. 56254228
3321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG250400.1

Sequence Viewer

Length: 1368 bp
ATGAAGATGCTAGTGGTATTGCTCTTGCTGCTGAGTTTGGGCGATGCTATTGGTGGAGAAGACGAGGATAGGCCTTGTGATTCTAAACTGCAATTAAAACCAAGGCCGCATAGTGTTTCCATTTTGGAGTTTGGTGCTGTTGGAGATGGCAAGACATTGAACACTCTTGCTTTCCAGAATGCCATTTTCTATCTCAAGTCTTTTGCTGACAAGGGCGGTGCACAGCTTTATGTGCCGCCCGGAAGATGGCTTACCGGAAGTTTCAACCTCACGAGCCATCTCACACTGTTTTTGGAAAAGGGTGCTGTCATTCTTGGATCTCAGGATCCATCTCATTGGGGTATTCTTGAACCCTTGCCCTCGTACGGTCGAGGGATTGAACTCCCAGGAGGGAGATATTGTAGCTTGATAAATGGATATATGTTACATGATGTGGTCATAACAGGTGATAATGGAACCATCAACGGTCAAGGATCAGTGTGGTGGGATTGGTTTAACTCTCATACTTTAAACCATAGCCGTCCTCATCTTGTAGAGTTAGTTTCGTCTAAAGATGTTGTTGTTTCAAACCTCACATTTGTGAATTCTCCTGCATACAACATTCATCCAGTCTATTGCAGTAATGTACATATTCACAACATCTCAGTCTCTGCTCCCCCGGAGTCCCCTTATACAGTCGGTATAGTCCCAGATTCTTCTGACAATGTGTGCATACAAGATTGCAGCATTGGCATGGGTTATGATGCCATTGTCCTCAAGAGTGGTTGGGACGAGTATGGCATTGCCTATGGAAGACCAACCACAAATGTACACATCAGACGGGTCCAGCTACAATCGTCTTCTGGTTCTTCTCTTGCCTTTGGTAGTGAGATGTCCGGTGGCATTTCTAATGTGCTTGTGGAGCAGGTCCACCTATACAACTCACTTAGTGGCATTCAGTTTAGAACAACAAAGGGTAGAGGTGGTTATATCAAAGATATCGTAATTTCAGATGTCAAAATGGAAAACATCGACGTGGCATTTGGTGCGTCTGGTCAGTTTGGTTCCCATCCGGACGACAACTTCGATCCTAATGCCCTTCCAGTATTGGATCACATCAACTTACAGGATGTGATTGGTACCAACATCACTATTGCTGGAAAATTCTCTGGGATCAAGGAATCCCCCTTCACTTCCTTTTGTCTGTCCAATATCTCCTTGTCAATCAATTCTGGTTCTTCTACCAATTCTTGGGTATGTTCAAATGTTTCTGGCTCTTCTGAGTCTGTCTTCCCCGAACCATGTTCCGCCCTTAAGTCATCGTATTCAAATTCTTCCTCAGCCTGCTTTTCGATACAGACCTTGAATGGAATAGCCGTGGCATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

455

Amino Acids

48.8

Weight (kDa)

5.46

Isoelectric Point (pI)

44.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 138 - 417 2.6e-27 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 895
Acc65I GGTACC 1 cut(s) 1118
AccB1I GGYRCC 1 cut(s) 1118
AccB7I CCANNNNNTGG 1 cut(s) 1230
AccIII TCCGGA 1 cut(s) 1051
AciI CCGC 4 cut(s) 107, 216, 236, 1287
AclWI GGATC 7 cut(s) 320, 325, 333, 481, 1061, 1098, 1160
AcsI RAATTY 3 cut(s) 583, 1142, 1309
AdeI CACNNNGTG 1 cut(s) 929
AfaI GTAC 4 cut(s) 365, 627, 810, 1120
AfiI CCNNNNNNNGG 3 cut(s) 246, 365, 1230
AflII CTTAAG 1 cut(s) 1292
AgsI TTSAA 8 cut(s) 160, 265, 350, 380, 567, 1242, 1308, 1345
AjiI CACGTC 1 cut(s) 1015
AjnI CCWGG 1 cut(s) 385
AleI CACNNNNGTG 1 cut(s) 578
AluBI AGCT 3 cut(s) 226, 405, 829
AluI AGCT 3 cut(s) 226, 405, 829
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 1 cut(s) 652
Alw44I GTGCAC 1 cut(s) 219
AlwI GGATC 7 cut(s) 320, 325, 333, 481, 1061, 1098, 1160
AlwNI CAGNNNCTG 1 cut(s) 650
Aor13HI TCCGGA 1 cut(s) 1051
AoxI GGCC 2 cut(s) 71, 104
ApaLI GTGCAC 1 cut(s) 219
ApeKI GCWGC 2 cut(s) 28, 723
ApoI RAATTY 3 cut(s) 583, 1142, 1309
ArsI GACNNNNNNTTYG 4 cut(s) 1004, 1036, 1046, 1078
Asp718I GGTACC 1 cut(s) 1118
AspS9I GGNCC 2 cut(s) 823, 907
AsuC2I CCSGG 2 cut(s) 240, 659
AsuHPI GGTGA 1 cut(s) 458
AvaII GGWCC 2 cut(s) 823, 907
BaeGI GKGCMC 1 cut(s) 223
BamHI GGATCC 1 cut(s) 325
BanI GGYRCC 1 cut(s) 1118
BarI GAAGNNNNNNTAC 2 cut(s) 235, 267
BauI CACGAG 1 cut(s) 271
BbsI GAAGAC 4 cut(s) 66, 799, 831, 1261
Bbv12I GWGCWC 1 cut(s) 223
BbvCI CCTCAGC 1 cut(s) 1318
BbvI GCAGC 2 cut(s) 15, 735
BccI CCATC 6 cut(s) 140, 240, 285, 337, 467, 1056
BceAI ACGGC 2 cut(s) 504, 1340
BcgI CGANNNNNNTGC 2 cut(s) 1055, 1089
BciT130I CCWGG 1 cut(s) 387
BcnI CCSGG 2 cut(s) 240, 659
BcoDI GTCTC 1 cut(s) 652
BfaI CTAG 1 cut(s) 11
BfrI CTTAAG 1 cut(s) 1292
BfuAI ACCTGC 1 cut(s) 895
BisI GCNGC 4 cut(s) 29, 107, 236, 724
BlsI GCNGC 4 cut(s) 30, 108, 237, 725
Bme1390I CCNGG 3 cut(s) 240, 387, 659
Bme18I GGWCC 2 cut(s) 823, 907
BmgBI CACGTC 1 cut(s) 1015
BmgT120I GGNCC 2 cut(s) 823, 907
BmiI GGNNCC 5 cut(s) 327, 457, 824, 1045, 1120
BmrFI CCNGG 3 cut(s) 240, 387, 659
BmsI GCATC 2 cut(s) 34, 733
BpiI GAAGAC 4 cut(s) 66, 799, 831, 1261
Bpu10I CCTNAGC 1 cut(s) 1318
BpuEI CTTGAG 2 cut(s) 179, 740
BpuMI CCSGG 2 cut(s) 240, 659
BsaJI CCNNGG 4 cut(s) 101, 385, 657, 1356
BsaWI WCCGGW 3 cut(s) 254, 875, 1051
BsaXI ACNNNNNCTCC 2 cut(s) 385, 415
Bsc4I CCNNNNNNNGG 3 cut(s) 246, 365, 1230
Bse1I ACTGG 2 cut(s) 608, 1082
Bse3DI GCAATG 1 cut(s) 780
BseAI TCCGGA 1 cut(s) 1051
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 4 cut(s) 101, 385, 657, 1356
BseGI GGATG 3 cut(s) 604, 1048, 1114
BseLI CCNNNNNNNGG 3 cut(s) 246, 365, 1230
BseMI GCAATG 1 cut(s) 780
BseMII CTCAG 5 cut(s) 23, 335, 657, 1251, 1332
BseNI ACTGG 2 cut(s) 608, 1082
BseSI GKGCMC 1 cut(s) 223
BseXI GCAGC 2 cut(s) 15, 735
Bsh1285I CGRYCG 1 cut(s) 370
BshFI GGCC 2 cut(s) 73, 106
BshNI GGYRCC 1 cut(s) 1118
BsiEI CGRYCG 1 cut(s) 370
BsiHKAI GWGCWC 1 cut(s) 223
BsiSI CCGG 5 cut(s) 240, 255, 659, 876, 1052
BsiWI CGTACG 1 cut(s) 363
BslFI GGGAC 3 cut(s) 649, 671, 782
BslI CCNNNNNNNGG 3 cut(s) 246, 365, 1230
BsmAI GTCTC 1 cut(s) 652
BsmFI GGGAC 3 cut(s) 649, 671, 782
BsmI GAATGC 2 cut(s) 184, 933
BsnI GGCC 2 cut(s) 73, 106
Bsp1286I GDGCHC 1 cut(s) 223
Bsp13I TCCGGA 1 cut(s) 1051
Bsp1407I TGTACA 2 cut(s) 625, 808
Bsp143I GATC 6 cut(s) 317, 325, 473, 1066, 1090, 1152
BspACI CCGC 4 cut(s) 107, 216, 236, 1287
BspANI GGCC 2 cut(s) 73, 106
BspCNI CTCAG 5 cut(s) 24, 334, 656, 1252, 1331
BspEI TCCGGA 1 cut(s) 1051
BspLI GGNNCC 5 cut(s) 327, 457, 824, 1045, 1120
BspMI ACCTGC 1 cut(s) 895
BspPI GGATC 7 cut(s) 320, 325, 333, 481, 1061, 1098, 1160
BspQI GCTCTTC 1 cut(s) 1261
BspT107I GGYRCC 1 cut(s) 1118
BspTI CTTAAG 1 cut(s) 1292
BsrDI GCAATG 1 cut(s) 780
BsrGI TGTACA 2 cut(s) 625, 808
BsrI ACTGG 2 cut(s) 608, 1082
BssECI CCNNGG 4 cut(s) 101, 385, 657, 1356
BssMI GATC 6 cut(s) 317, 325, 473, 1066, 1090, 1152
BssSI CACGAG 1 cut(s) 271
BssT1I CCWWGG 1 cut(s) 101
Bst2BI CACGAG 1 cut(s) 271
Bst2UI CCWGG 1 cut(s) 387
Bst4CI ACNGT 4 cut(s) 288, 368, 467, 676
Bst6I CTCTTC 1 cut(s) 1261
BstAFI CTTAAG 1 cut(s) 1292
BstAPI GCANNNNNTGC 1 cut(s) 1025
BstAUI TGTACA 2 cut(s) 625, 808
BstC8I GCNNGC 1 cut(s) 1324
BstDEI CTNAG 6 cut(s) 32, 321, 643, 926, 1260, 1318
BstDSI CCRYGG 1 cut(s) 1356
BstF5I GGATG 3 cut(s) 604, 1048, 1114
BstKTI GATC 6 cut(s) 320, 328, 476, 1069, 1093, 1155
BstMAI GTCTC 1 cut(s) 652
BstMBI GATC 6 cut(s) 317, 325, 473, 1066, 1090, 1152
BstMCI CGRYCG 1 cut(s) 370
BstMWI GCNNNNNNNGC 5 cut(s) 28, 232, 729, 901, 1025
BstNI CCWGG 1 cut(s) 387
BstSCI CCNGG 3 cut(s) 238, 385, 657
BstSLI GKGCMC 1 cut(s) 223
BstV1I GCAGC 2 cut(s) 15, 735
BstV2I GAAGAC 4 cut(s) 66, 799, 831, 1261
BstX2I RGATCY 2 cut(s) 317, 325
BstXI CCANNNNNNTGG 1 cut(s) 336
BstYI RGATCY 2 cut(s) 317, 325
BsuRI GGCC 2 cut(s) 73, 106
BtgI CCRYGG 1 cut(s) 1356
BtgZI GCGATG 1 cut(s) 57
BtrI CACGTC 1 cut(s) 1015
BtsCI GGATG 3 cut(s) 604, 1048, 1114
BtsIMutI CAGTG 2 cut(s) 284, 483
BveI ACCTGC 1 cut(s) 895
Cac8I GCNNGC 1 cut(s) 1324
CaiI CAGNNNCTG 1 cut(s) 650
Cfr13I GGNCC 2 cut(s) 823, 907
CseI GACGC 1 cut(s) 1017
Csp6I GTAC 4 cut(s) 364, 626, 809, 1119
CviAII CATG 3 cut(s) 428, 733, 1281
CviQI GTAC 4 cut(s) 364, 626, 809, 1119
DdeI CTNAG 6 cut(s) 32, 321, 643, 926, 1260, 1318
DpnI GATC 6 cut(s) 319, 327, 475, 1068, 1092, 1154
DpnII GATC 6 cut(s) 317, 325, 473, 1066, 1090, 1152
DraI TTTAAA 1 cut(s) 510
DraIII CACNNNGTG 1 cut(s) 929
Eam1104I CTCTTC 1 cut(s) 1261
EarI CTCTTC 1 cut(s) 1261
EciI GGCGGA 1 cut(s) 1276
Eco130I CCWWGG 1 cut(s) 101
Eco147I AGGCCT 1 cut(s) 73
Eco32I GATATC 1 cut(s) 979
Eco47I GGWCC 2 cut(s) 823, 907
EcoRI GAATTC 1 cut(s) 583
EcoRII CCWGG 1 cut(s) 385
EcoRV GATATC 1 cut(s) 979
EcoT14I CCWWGG 1 cut(s) 101
ErhI CCWWGG 1 cut(s) 101
FaeI CATG 3 cut(s) 431, 736, 1284
FaqI GGGAC 3 cut(s) 649, 671, 782
FatI CATG 3 cut(s) 427, 732, 1280
Fnu4HI GCNGC 4 cut(s) 29, 107, 236, 724
FokI GGATG 3 cut(s) 591, 1035, 1121
Fsp4HI GCNGC 4 cut(s) 29, 107, 236, 724
FspBI CTAG 1 cut(s) 11
GluI GCNGC 4 cut(s) 29, 107, 236, 724
HaeIII GGCC 2 cut(s) 73, 106
HapII CCGG 5 cut(s) 240, 255, 659, 876, 1052
HgaI GACGC 1 cut(s) 1017
Hin1II CATG 3 cut(s) 431, 736, 1284
HinfI GANTC 5 cut(s) 80, 662, 692, 1160, 1262
HpaII CCGG 5 cut(s) 240, 255, 659, 876, 1052
HphI GGTGA 1 cut(s) 458
Hpy166II GTNNAC 3 cut(s) 221, 811, 910
Hpy188I TCNGA 4 cut(s) 700, 818, 991, 1261
Hpy188III TCNNGA 6 cut(s) 175, 271, 323, 347, 757, 1052
Hpy8I GTNNAC 3 cut(s) 221, 811, 910
Hpy99I CGWCG 1 cut(s) 1016
HpyAV CCTTC 2 cut(s) 1088, 1177
HpyCH4III ACNGT 4 cut(s) 288, 368, 467, 676
HpyCH4IV ACGT 1 cut(s) 1014
HpyCH4V TGCA 6 cut(s) 91, 221, 593, 618, 711, 723
HpyF10VI GCNNNNNNNGC 5 cut(s) 28, 232, 729, 901, 1025
HpyF3I CTNAG 6 cut(s) 32, 321, 643, 926, 1260, 1318
HpySE526I ACGT 1 cut(s) 1014
Hsp92II CATG 3 cut(s) 431, 736, 1284
Kpn2I TCCGGA 1 cut(s) 1051
KpnI GGTACC 1 cut(s) 1122
Kzo9I GATC 6 cut(s) 317, 325, 473, 1066, 1090, 1152
LguI GCTCTTC 1 cut(s) 1261
LmnI GCTCC 2 cut(s) 658, 901
Lsp1109I GCAGC 2 cut(s) 15, 735
LweI GCATC 2 cut(s) 34, 733
MaeI CTAG 1 cut(s) 11
MaeII ACGT 1 cut(s) 1014
MaeIII GTNAC 1 cut(s) 423
MalI GATC 6 cut(s) 319, 327, 475, 1068, 1092, 1154
MboI GATC 6 cut(s) 317, 325, 473, 1066, 1090, 1152
MflI RGATCY 2 cut(s) 317, 325
MhlI GDGCHC 1 cut(s) 223
MluCI AATT 7 cut(s) 92, 583, 984, 1142, 1207, 1225, 1309
MlyI GAGTC 2 cut(s) 671, 1271
MmeI TCCRAC 1 cut(s) 121
MroI TCCGGA 1 cut(s) 1051
MseI TTAA 4 cut(s) 95, 495, 509, 1293
MslI CAYNNNNRTG 3 cut(s) 578, 731, 1013
MspCI CTTAAG 1 cut(s) 1292
MspI CCGG 5 cut(s) 240, 255, 659, 876, 1052
MspR9I CCNGG 3 cut(s) 240, 387, 659
Mva1269I GAATGC 2 cut(s) 184, 933
MvaI CCWGG 1 cut(s) 387
MwoI GCNNNNNNNGC 5 cut(s) 28, 232, 729, 901, 1025
NciI CCSGG 2 cut(s) 240, 659
NdeII GATC 6 cut(s) 317, 325, 473, 1066, 1090, 1152
NlaIII CATG 3 cut(s) 431, 736, 1284
NlaIV GGNNCC 5 cut(s) 327, 457, 824, 1045, 1120
OliI CACNNNNGTG 1 cut(s) 578
PceI AGGCCT 1 cut(s) 73
PciSI GCTCTTC 1 cut(s) 1261
PcsI WCGNNNNNNNCGW 1 cut(s) 1062
PctI GAATGC 2 cut(s) 184, 933
PfeI GAWTC 3 cut(s) 80, 692, 1160
Pfl23II CGTACG 1 cut(s) 363
PflMI CCANNNNNTGG 1 cut(s) 1230
PkrI GCNGC 4 cut(s) 30, 108, 237, 725
PleI GAGTC 2 cut(s) 670, 1270
PpsI GAGTC 2 cut(s) 670, 1270
Psp6I CCWGG 1 cut(s) 385
PspGI CCWGG 1 cut(s) 385
PspLI CGTACG 1 cut(s) 363
PspN4I GGNNCC 5 cut(s) 327, 457, 824, 1045, 1120
PspPI GGNCC 2 cut(s) 823, 907
PstNI CAGNNNCTG 1 cut(s) 650
PsuI RGATCY 2 cut(s) 317, 325
RsaI GTAC 4 cut(s) 365, 627, 810, 1120
RsaNI GTAC 4 cut(s) 364, 626, 809, 1119
RseI CAYNNNNRTG 3 cut(s) 578, 731, 1013
SapI GCTCTTC 1 cut(s) 1261
SaqAI TTAA 4 cut(s) 95, 495, 509, 1293
SatI GCNGC 4 cut(s) 29, 107, 236, 724
Sau3AI GATC 6 cut(s) 317, 325, 473, 1066, 1090, 1152
Sau96I GGNCC 2 cut(s) 823, 907
SchI GAGTC 2 cut(s) 671, 1271
ScrFI CCNGG 3 cut(s) 240, 387, 659
SduI GDGCHC 1 cut(s) 223
SfaNI GCATC 2 cut(s) 34, 733
SinI GGWCC 2 cut(s) 823, 907
SmiMI CAYNNNNRTG 3 cut(s) 578, 731, 1013
SmlI CTYRAG 3 cut(s) 194, 755, 1292
SmoI CTYRAG 3 cut(s) 194, 755, 1292
Sse9I AATT 7 cut(s) 92, 583, 984, 1142, 1207, 1225, 1309
SseBI AGGCCT 1 cut(s) 73
SsiI CCGC 4 cut(s) 107, 216, 236, 1287
SspMI CTAG 1 cut(s) 11
StuI AGGCCT 1 cut(s) 73
StyD4I CCNGG 3 cut(s) 238, 385, 657
StyI CCWWGG 1 cut(s) 101
TaaI ACNGT 4 cut(s) 288, 368, 467, 676
TaiI ACGT 1 cut(s) 1017
TaqI TCGA 4 cut(s) 370, 1011, 1065, 1331
TasI AATT 7 cut(s) 92, 583, 984, 1142, 1207, 1225, 1309
TatI WGTACW 2 cut(s) 625, 808
TauI GCSGC 2 cut(s) 109, 238
TfiI GAWTC 3 cut(s) 80, 692, 1160
Tru1I TTAA 4 cut(s) 95, 495, 509, 1293
Tru9I TTAA 4 cut(s) 95, 495, 509, 1293
TscAI CASTG 2 cut(s) 291, 483
TseI GCWGC 2 cut(s) 28, 723
TspDTI ATGAA 2 cut(s) 17, 593
TspRI CASTG 2 cut(s) 291, 483
Van91I CCANNNNNTGG 1 cut(s) 1230
Vha464I CTTAAG 1 cut(s) 1292
VneI GTGCAC 1 cut(s) 219
VpaK11BI GGWCC 2 cut(s) 823, 907
XapI RAATTY 3 cut(s) 583, 1142, 1309
XspI CTAG 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.