FvH4_4g18930

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008733
Physical Location & Seq
Forward (+)
22703736 .. 22707253
3518 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g18930.t1

Sequence Viewer

Length: 1374 bp
ATGAAGATGCTAGTGGTATTGCTCTTGTTGTTGAGTTTGGGCAATGCAATCAAACTCGGTGGAGAAGACGAAGATTGGCCTTGTGATTCTAAACTGCAATTAAAACCAAGGCCGCATAGTGTTTCCATTTTGGAGTTTGGTGCTGTTGGGGATGGCAAAACATTGAACACTCTTGCTTTCCAGAATGCCATTTTCTATCTCAAGTCTTTTGCTGACAAGGGCGGTGCACAGCTTTATGTGCCGCCTGGAAGATGGCTTACCGGAAGTTTCAACCTTACAAGCAATCTCACGCTATTTTTGGAGAAGGGTGCTGTCATTCTTGGATCTCAGGATCCATCTCATTGGGATATTCTTGAGCCCTTGCCCTCGTACGGTCGAGGGATTGAACTCCCAGGAGGGAGATATCGTAGCTTGATAAATGGATATATGTTACAGGATGTGGTCATAACCGGTGATAATGGAACCATCAACGGTCAAGGATCAGTGTGGTGGGATTGGTTTAGCTCTCATACTTTAAACCATAGCCGTCCTCATCTTGTAGAGATAGTTTCTTCTAAAGATGTTGTTGTTTCAAACCTCACGTTTGTCAATTCTCCTGCATACAACATTCATCCAGTCTACTGCAGTAATGTACATATTCACAACATCTCTGTCTCTGCTCCCCCGGAGTCCCCTTATACAGTTGGTATAGTCCCAGATTCTTCTAATAATGTGTGCATACAAGATTGCAGCATTGGCATGGGGTATGATGCCATTGTCCTCAAGAGTGGCTGGGATGAATACGGCATTGCCTATGGAAGACCAACCACAAATGTGCACATCAGACGGGTCCAGCTACAATCATCTTCTGGTTCTTCTCTTGCCTTTGGTAGTGAGATGTCCGGTGGCATTTCTAATGTGCTTGTGGAGCAGGTCCACCTATACAACTCACTTAGTGGCATTCAGTTTAGAACAACAAAGGGTAGAGGTGGTTATATCAGAAATATAATCATTTCAGATGTCAAAATGGAAAACATCGGCGTGGCATTTGGTGCGTCTGGTCAGTTTGGTTCCCATCCGGATGACAACTTTGATCCTAATGCCCTTCCAGTATTGGATCACATCAGCTTACAGGATGTGATCGGTACCAACATCTCTATTGCTGGAAAATTCACTGGGATCAAGGAATCCCCCTTCACTTCCTTTTGTTTGTCCAACATCTCCTTGTCAATCAGTTCAGGTTCTTCTACAAATTCTTGGGTATGTTCAAATGTTTCTGGCTTTTCTGATTCTGTCTTTCCTGAACCATGTTCTGCCCTTAAGTCCTCACATTCAAATTCTTCCTCAGACTGCTTTTCGATACAGACCTTGAATGGAAAAGCCGTAGCATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

458

Amino Acids

49.13

Weight (kDa)

5.78

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 138 - 419 6.9e-29 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 901
Acc65I GGTACC 1 cut(s) 1124
AccB1I GGYRCC 1 cut(s) 1124
AccI GTMKAC 1 cut(s) 618
AccIII TCCGGA 1 cut(s) 1057
AciI CCGC 3 cut(s) 113, 222, 242
AclWI GGATC 7 cut(s) 326, 331, 339, 487, 1067, 1104, 1166
AcsI RAATTY 3 cut(s) 1148, 1231, 1315
AdeI CACNNNGTG 1 cut(s) 935
AfaI GTAC 3 cut(s) 371, 633, 1126
AfiI CCNNNNNNNGG 1 cut(s) 371
AflII CTTAAG 1 cut(s) 1298
AgeI ACCGGT 1 cut(s) 449
AgsI TTSAA 7 cut(s) 166, 271, 386, 573, 1248, 1314, 1351
AjnI CCWGG 2 cut(s) 244, 391
AleI CACNNNNGTG 1 cut(s) 812
AluBI AGCT 5 cut(s) 232, 411, 504, 835, 1107
AluI AGCT 5 cut(s) 232, 411, 504, 835, 1107
Alw21I GWGCWC 2 cut(s) 229, 819
Alw26I GTCTC 1 cut(s) 658
Alw44I GTGCAC 2 cut(s) 225, 815
AlwI GGATC 7 cut(s) 326, 331, 339, 487, 1067, 1104, 1166
Aor13HI TCCGGA 1 cut(s) 1057
AoxI GGCC 2 cut(s) 77, 110
ApaLI GTGCAC 2 cut(s) 225, 815
ApeKI GCWGC 1 cut(s) 729
ApoI RAATTY 3 cut(s) 1148, 1231, 1315
AsiGI ACCGGT 1 cut(s) 449
Asp718I GGTACC 1 cut(s) 1124
AspS9I GGNCC 2 cut(s) 829, 913
AsuC2I CCSGG 1 cut(s) 665
AsuHPI GGTGA 1 cut(s) 464
AvaII GGWCC 2 cut(s) 829, 913
BaeGI GKGCMC 2 cut(s) 229, 819
BamHI GGATCC 1 cut(s) 331
BanI GGYRCC 1 cut(s) 1124
BanII GRGCYC 1 cut(s) 360
BarI GAAGNNNNNNTAC 2 cut(s) 241, 273
BbsI GAAGAC 2 cut(s) 72, 805
Bbv12I GWGCWC 2 cut(s) 229, 819
BbvI GCAGC 1 cut(s) 741
BccI CCATC 5 cut(s) 146, 246, 343, 473, 1062
BceAI ACGGC 3 cut(s) 510, 799, 1346
BciT130I CCWGG 2 cut(s) 246, 393
BcnI CCSGG 1 cut(s) 665
BcoDI GTCTC 1 cut(s) 658
BfaI CTAG 1 cut(s) 11
BfmI CTRYAG 1 cut(s) 622
BfrI CTTAAG 1 cut(s) 1298
BfuAI ACCTGC 1 cut(s) 901
BisI GCNGC 3 cut(s) 113, 242, 730
BlsI GCNGC 3 cut(s) 114, 243, 731
Bme1390I CCNGG 3 cut(s) 246, 393, 665
Bme18I GGWCC 2 cut(s) 829, 913
BmgT120I GGNCC 2 cut(s) 829, 913
BmiI GGNNCC 5 cut(s) 333, 463, 830, 1051, 1126
BmrFI CCNGG 3 cut(s) 246, 393, 665
BmrI ACTGGG 1 cut(s) 1164
BmsI GCATC 1 cut(s) 739
BmuI ACTGGG 1 cut(s) 1164
BpiI GAAGAC 2 cut(s) 72, 805
BpuEI CTTGAG 3 cut(s) 185, 374, 746
BpuMI CCSGG 1 cut(s) 665
BsaJI CCNNGG 3 cut(s) 107, 391, 663
BsaWI WCCGGW 4 cut(s) 260, 449, 881, 1057
BsaXI ACNNNNNCTCC 2 cut(s) 391, 421
Bsc4I CCNNNNNNNGG 1 cut(s) 371
Bse118I RCCGGY 1 cut(s) 449
Bse1I ACTGG 3 cut(s) 614, 1088, 1159
Bse3DI GCAATG 2 cut(s) 49, 786
BseAI TCCGGA 1 cut(s) 1057
BseBI CCWGG 2 cut(s) 246, 393
BseDI CCNNGG 3 cut(s) 107, 391, 663
BseGI GGATG 7 cut(s) 157, 442, 610, 781, 1054, 1066, 1120
BseLI CCNNNNNNNGG 1 cut(s) 371
BseMI GCAATG 2 cut(s) 49, 786
BseMII CTCAG 2 cut(s) 341, 1338
BseNI ACTGG 3 cut(s) 614, 1088, 1159
BseSI GKGCMC 2 cut(s) 229, 819
BseXI GCAGC 1 cut(s) 741
BseYI CCCAGC 1 cut(s) 771
Bsh1285I CGRYCG 1 cut(s) 376
BshFI GGCC 2 cut(s) 79, 112
BshNI GGYRCC 1 cut(s) 1124
BshTI ACCGGT 1 cut(s) 449
BsiEI CGRYCG 1 cut(s) 376
BsiHKAI GWGCWC 2 cut(s) 229, 819
BsiSI CCGG 5 cut(s) 261, 450, 665, 882, 1058
BsiWI CGTACG 1 cut(s) 369
BslFI GGGAC 2 cut(s) 655, 677
BslI CCNNNNNNNGG 1 cut(s) 371
BsmAI GTCTC 1 cut(s) 658
BsmFI GGGAC 2 cut(s) 655, 677
BsmI GAATGC 2 cut(s) 190, 939
BsnI GGCC 2 cut(s) 79, 112
Bsp1286I GDGCHC 3 cut(s) 229, 360, 819
Bsp13I TCCGGA 1 cut(s) 1057
Bsp1407I TGTACA 1 cut(s) 631
Bsp143I GATC 7 cut(s) 323, 331, 479, 1072, 1096, 1119, 1158
BspACI CCGC 3 cut(s) 113, 222, 242
BspANI GGCC 2 cut(s) 79, 112
BspCNI CTCAG 2 cut(s) 340, 1337
BspEI TCCGGA 1 cut(s) 1057
BspLI GGNNCC 5 cut(s) 333, 463, 830, 1051, 1126
BspMAI CTGCAG 1 cut(s) 626
BspMI ACCTGC 1 cut(s) 901
BspPI GGATC 7 cut(s) 326, 331, 339, 487, 1067, 1104, 1166
BspT107I GGYRCC 1 cut(s) 1124
BspTI CTTAAG 1 cut(s) 1298
BsrDI GCAATG 2 cut(s) 49, 786
BsrFI RCCGGY 1 cut(s) 449
BsrGI TGTACA 1 cut(s) 631
BsrI ACTGG 3 cut(s) 614, 1088, 1159
BssAI RCCGGY 1 cut(s) 449
BssECI CCNNGG 3 cut(s) 107, 391, 663
BssMI GATC 7 cut(s) 323, 331, 479, 1072, 1096, 1119, 1158
BssT1I CCWWGG 1 cut(s) 107
Bst2UI CCWGG 2 cut(s) 246, 393
Bst4CI ACNGT 3 cut(s) 374, 473, 682
BstAFI CTTAAG 1 cut(s) 1298
BstAPI GCANNNNNTGC 1 cut(s) 1031
BstAUI TGTACA 1 cut(s) 631
BstDEI CTNAG 3 cut(s) 327, 932, 1324
BstF5I GGATG 7 cut(s) 157, 442, 610, 781, 1054, 1066, 1120
BstKTI GATC 7 cut(s) 326, 334, 482, 1075, 1099, 1122, 1161
BstMAI GTCTC 1 cut(s) 658
BstMBI GATC 7 cut(s) 323, 331, 479, 1072, 1096, 1119, 1158
BstMCI CGRYCG 1 cut(s) 376
BstMWI GCNNNNNNNGC 4 cut(s) 238, 735, 907, 1031
BstNI CCWGG 2 cut(s) 246, 393
BstSCI CCNGG 3 cut(s) 244, 391, 663
BstSFI CTRYAG 1 cut(s) 622
BstSLI GKGCMC 2 cut(s) 229, 819
BstV1I GCAGC 1 cut(s) 741
BstV2I GAAGAC 2 cut(s) 72, 805
BstX2I RGATCY 2 cut(s) 323, 331
BstXI CCANNNNNNTGG 1 cut(s) 342
BstYI RGATCY 2 cut(s) 323, 331
BsuRI GGCC 2 cut(s) 79, 112
BtsCI GGATG 7 cut(s) 157, 442, 610, 781, 1054, 1066, 1120
BtsIMutI CAGTG 2 cut(s) 489, 1152
BveI ACCTGC 1 cut(s) 901
Cfr10I RCCGGY 1 cut(s) 449
Cfr13I GGNCC 2 cut(s) 829, 913
CseI GACGC 1 cut(s) 1023
Csp6I GTAC 3 cut(s) 370, 632, 1125
CspAI ACCGGT 1 cut(s) 449
CspCI CAANNNNNGTGG 2 cut(s) 40, 75
CviAII CATG 2 cut(s) 739, 1287
CviQI GTAC 3 cut(s) 370, 632, 1125
DdeI CTNAG 3 cut(s) 327, 932, 1324
DpnI GATC 7 cut(s) 325, 333, 481, 1074, 1098, 1121, 1160
DpnII GATC 7 cut(s) 323, 331, 479, 1072, 1096, 1119, 1158
DraI TTTAAA 1 cut(s) 516
DraIII CACNNNGTG 1 cut(s) 935
Eco130I CCWWGG 1 cut(s) 107
Eco24I GRGCYC 1 cut(s) 360
Eco32I GATATC 1 cut(s) 404
Eco47I GGWCC 2 cut(s) 829, 913
EcoRII CCWGG 2 cut(s) 244, 391
EcoRV GATATC 1 cut(s) 404
EcoT14I CCWWGG 1 cut(s) 107
EcoT38I GRGCYC 1 cut(s) 360
ErhI CCWWGG 1 cut(s) 107
FaeI CATG 2 cut(s) 742, 1290
FaqI GGGAC 2 cut(s) 655, 677
FatI CATG 2 cut(s) 738, 1286
FblI GTMKAC 1 cut(s) 618
Fnu4HI GCNGC 3 cut(s) 113, 242, 730
FokI GGATG 7 cut(s) 164, 449, 597, 788, 1041, 1073, 1127
FriOI GRGCYC 1 cut(s) 360
Fsp4HI GCNGC 3 cut(s) 113, 242, 730
FspBI CTAG 1 cut(s) 11
GluI GCNGC 3 cut(s) 113, 242, 730
GsaI CCCAGC 1 cut(s) 775
HaeIII GGCC 2 cut(s) 79, 112
HapII CCGG 5 cut(s) 261, 450, 665, 882, 1058
HgaI GACGC 1 cut(s) 1023
Hin1II CATG 2 cut(s) 742, 1290
HinfI GANTC 5 cut(s) 86, 668, 698, 1166, 1268
HpaII CCGG 5 cut(s) 261, 450, 665, 882, 1058
HphI GGTGA 1 cut(s) 464
Hpy166II GTNNAC 4 cut(s) 227, 619, 817, 916
Hpy188I TCNGA 5 cut(s) 824, 980, 997, 1267, 1327
Hpy188III TCNNGA 6 cut(s) 181, 329, 353, 763, 1058, 1280
Hpy8I GTNNAC 4 cut(s) 227, 619, 817, 916
HpyAV CCTTC 3 cut(s) 298, 1094, 1183
HpyCH4III ACNGT 3 cut(s) 374, 473, 682
HpyCH4IV ACGT 1 cut(s) 581
HpyCH4V TGCA 8 cut(s) 47, 97, 227, 599, 624, 717, 729, 817
HpyF10VI GCNNNNNNNGC 4 cut(s) 238, 735, 907, 1031
HpyF3I CTNAG 3 cut(s) 327, 932, 1324
HpySE526I ACGT 1 cut(s) 581
Hsp92II CATG 2 cut(s) 742, 1290
Kpn2I TCCGGA 1 cut(s) 1057
KpnI GGTACC 1 cut(s) 1128
Kzo9I GATC 7 cut(s) 323, 331, 479, 1072, 1096, 1119, 1158
LmnI GCTCC 2 cut(s) 664, 907
Lsp1109I GCAGC 1 cut(s) 741
LweI GCATC 1 cut(s) 739
MaeI CTAG 1 cut(s) 11
MaeII ACGT 1 cut(s) 581
MaeIII GTNAC 1 cut(s) 429
MalI GATC 7 cut(s) 325, 333, 481, 1074, 1098, 1121, 1160
MboI GATC 7 cut(s) 323, 331, 479, 1072, 1096, 1119, 1158
MflI RGATCY 2 cut(s) 323, 331
MhlI GDGCHC 3 cut(s) 229, 360, 819
MluCI AATT 5 cut(s) 98, 589, 1148, 1231, 1315
MlyI GAGTC 1 cut(s) 677
MmeI TCCRAC 1 cut(s) 1218
MnlI CCTC 9 cut(s) 371, 376, 389, 540, 587, 770, 959, 1315, 1333
MroI TCCGGA 1 cut(s) 1057
MseI TTAA 3 cut(s) 101, 515, 1299
MslI CAYNNNNRTG 4 cut(s) 737, 812, 1019, 1059
MspCI CTTAAG 1 cut(s) 1298
MspI CCGG 5 cut(s) 261, 450, 665, 882, 1058
MspR9I CCNGG 3 cut(s) 246, 393, 665
Mva1269I GAATGC 2 cut(s) 190, 939
MvaI CCWGG 2 cut(s) 246, 393
MwoI GCNNNNNNNGC 4 cut(s) 238, 735, 907, 1031
NciI CCSGG 1 cut(s) 665
NdeII GATC 7 cut(s) 323, 331, 479, 1072, 1096, 1119, 1158
NlaIII CATG 2 cut(s) 742, 1290
NlaIV GGNNCC 5 cut(s) 333, 463, 830, 1051, 1126
OliI CACNNNNGTG 1 cut(s) 812
PctI GAATGC 2 cut(s) 190, 939
PfeI GAWTC 4 cut(s) 86, 698, 1166, 1268
Pfl23II CGTACG 1 cut(s) 369
PinAI ACCGGT 1 cut(s) 449
PkrI GCNGC 3 cut(s) 114, 243, 731
PleI GAGTC 1 cut(s) 676
PpsI GAGTC 1 cut(s) 676
Psp6I CCWGG 2 cut(s) 244, 391
PspFI CCCAGC 1 cut(s) 771
PspGI CCWGG 2 cut(s) 244, 391
PspLI CGTACG 1 cut(s) 369
PspN4I GGNNCC 5 cut(s) 333, 463, 830, 1051, 1126
PspPI GGNCC 2 cut(s) 829, 913
PstI CTGCAG 1 cut(s) 626
PsuI RGATCY 2 cut(s) 323, 331
RsaI GTAC 3 cut(s) 371, 633, 1126
RsaNI GTAC 3 cut(s) 370, 632, 1125
RseI CAYNNNNRTG 4 cut(s) 737, 812, 1019, 1059
SaqAI TTAA 3 cut(s) 101, 515, 1299
SatI GCNGC 3 cut(s) 113, 242, 730
Sau3AI GATC 7 cut(s) 323, 331, 479, 1072, 1096, 1119, 1158
Sau96I GGNCC 2 cut(s) 829, 913
SchI GAGTC 1 cut(s) 677
ScrFI CCNGG 3 cut(s) 246, 393, 665
SduI GDGCHC 3 cut(s) 229, 360, 819
SfaNI GCATC 1 cut(s) 739
SfcI CTRYAG 1 cut(s) 622
SinI GGWCC 2 cut(s) 829, 913
SmiMI CAYNNNNRTG 4 cut(s) 737, 812, 1019, 1059
SmlI CTYRAG 4 cut(s) 200, 353, 761, 1298
SmoI CTYRAG 4 cut(s) 200, 353, 761, 1298
Sse9I AATT 5 cut(s) 98, 589, 1148, 1231, 1315
SsiI CCGC 3 cut(s) 113, 222, 242
SspMI CTAG 1 cut(s) 11
StyD4I CCNGG 3 cut(s) 244, 391, 663
StyI CCWWGG 1 cut(s) 107
TaaI ACNGT 3 cut(s) 374, 473, 682
TaiI ACGT 1 cut(s) 584
TaqI TCGA 2 cut(s) 376, 1337
TasI AATT 5 cut(s) 98, 589, 1148, 1231, 1315
TatI WGTACW 1 cut(s) 631
TauI GCSGC 2 cut(s) 115, 244
TfiI GAWTC 4 cut(s) 86, 698, 1166, 1268
Tru1I TTAA 3 cut(s) 101, 515, 1299
Tru9I TTAA 3 cut(s) 101, 515, 1299
TscAI CASTG 2 cut(s) 489, 1159
TseI GCWGC 1 cut(s) 729
TspDTI ATGAA 3 cut(s) 17, 599, 792
TspRI CASTG 2 cut(s) 489, 1159
Vha464I CTTAAG 1 cut(s) 1298
VneI GTGCAC 2 cut(s) 225, 815
VpaK11BI GGWCC 2 cut(s) 829, 913
XapI RAATTY 3 cut(s) 1148, 1231, 1315
XmiI GTMKAC 1 cut(s) 618
XspI CTAG 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.