RLG00000007529

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0008733
Physical Location & Seq
Reverse (-)
17866596 .. 17869070
2475 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007529

Sequence Viewer

Length: 1362 bp
ATGCTAGTGGTATTGCTCTTGCTGCTGAGTTTGGGCGATGCTATTGGTGGAGAAGACGAGGATAGGCCTTGTGATTCTAAACTGCAATTAAAACCAAGGCCGCATAGTGTTTCCATTTTGGAGTTTGGTGCTGTTGGTGATGGCAAGACATTGAACACTCTTGCTTTCCAGAATGCCATTTTCTATCTCAAGTCTTTTGCTGACAAGGGCGGTGCACAGCTTTATGTGCCGCCCGGAAGATGGCTTACCGGAAGTTTCAACCTCACGAGCCATCTCACACTGTTTTTGGAAAAGGGTGCTGTCATTCTTGGATCTCAGGATCCATCTCATTGGGGTATTCTTGAACCCTTGCCCTCGTACGGTCGAGGGATTGAACTCCCAGGAGGGAGATATTGTAGCTTGATAAATGGATATATGTTACATGATGTGGTCATAACAGGTGATAATGGAACCATCAACGGTCAAGGATCAGTGTGGTGGGATTGGTTTAACTCTCATACTTTAAACCATAGCCGTCCTCATCTTGTAGAGTTTGTTTCGTCTAAAGATGTTGTTGTTTCAAACCTCACATTTGTGAATTCTCCTGCATACAACATTCATCCAGTCTATTGCAGTAATGTACATATTCACAACATCTCAGTCTCTGCTCCCCCGGAGTCCCCTTATACAGTCGGTATAGTCCCAGATTCTTCTGACAATGTGTGCATACAAGATTGCAGCATTGGCATGGGTTATGATGCCATTGTCCTCAAGAGTGGTTGGGACGAGTATGGCATTGCCTATGGAAGACCAACCACAAATGTACACATCAGACGGGTCCAGCTACAATCGTCTTCTGGTTCTTCTCTTGCCTTTGGTAGTGAGATGTCCGGTGGCATTTCTAATGTGCTTGTGGAGCAGGTCCACCTATACAACTCACTTAGTGGCATTCAGTTTAGAACAACAAAGGGTAGAGGTGGTTATATCAAAGATATCGTAATTTCAGATGTCCAAATGGAAAACATCGACGTGGCATTTGGTGCATCTGGTCAGTTTGGTTCCCATCCGGATGACAACTTCGATCCTAATGCCCTTCCGGTATTGGATCACATCAACTTACAGGATGTGATTGGTACCAACATCACTATTGCTGGAAAATTCTCTGGGATCAAGGAATCCCCCTTCACTTCCTTTTGTCTGTCCAATATCTCCTTGTCAATCAATTCTGGTTCTTCCACCAATTCTTGGGTATGTTCAAATGTTTCTGGCTCTTCTGAGTCTGTCTTCCCTGAACCATGTTCTGCCCTCAAGTCATCGTATTCAAATTCTTCCTCAGCCTGCTTTTCGATACAGACCTTGAATGGAATAGCCGTGGCATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

454

Amino Acids

48.58

Weight (kDa)

5.27

Isoelectric Point (pI)

43.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 136 - 415 1.6e-27 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 889
Acc65I GGTACC 1 cut(s) 1112
AccB1I GGYRCC 1 cut(s) 1112
AccB7I CCANNNNNTGG 1 cut(s) 1224
AccIII TCCGGA 1 cut(s) 1045
AciI CCGC 3 cut(s) 101, 210, 230
AclWI GGATC 7 cut(s) 314, 319, 327, 475, 1055, 1092, 1154
AcsI RAATTY 3 cut(s) 577, 1136, 1303
AdeI CACNNNGTG 1 cut(s) 923
AfaI GTAC 4 cut(s) 359, 621, 804, 1114
AfiI CCNNNNNNNGG 3 cut(s) 240, 359, 1224
AgsI TTSAA 8 cut(s) 154, 259, 344, 374, 561, 1236, 1302, 1339
AjiI CACGTC 1 cut(s) 1009
AjnI CCWGG 1 cut(s) 379
AleI CACNNNNGTG 1 cut(s) 572
AluBI AGCT 3 cut(s) 220, 399, 823
AluI AGCT 3 cut(s) 220, 399, 823
Alw21I GWGCWC 1 cut(s) 217
Alw26I GTCTC 1 cut(s) 646
Alw44I GTGCAC 1 cut(s) 213
AlwI GGATC 7 cut(s) 314, 319, 327, 475, 1055, 1092, 1154
AlwNI CAGNNNCTG 1 cut(s) 644
Aor13HI TCCGGA 1 cut(s) 1045
AoxI GGCC 2 cut(s) 65, 98
ApaLI GTGCAC 1 cut(s) 213
ApeKI GCWGC 2 cut(s) 22, 717
ApoI RAATTY 3 cut(s) 577, 1136, 1303
ArsI GACNNNNNNTTYG 2 cut(s) 998, 1030
Asp718I GGTACC 1 cut(s) 1112
AspS9I GGNCC 2 cut(s) 817, 901
AsuC2I CCSGG 2 cut(s) 234, 653
AsuHPI GGTGA 2 cut(s) 149, 452
AvaII GGWCC 2 cut(s) 817, 901
BaeGI GKGCMC 1 cut(s) 217
BamHI GGATCC 1 cut(s) 319
BanI GGYRCC 1 cut(s) 1112
BarI GAAGNNNNNNTAC 2 cut(s) 229, 261
BauI CACGAG 1 cut(s) 265
BbsI GAAGAC 4 cut(s) 60, 793, 825, 1255
Bbv12I GWGCWC 1 cut(s) 217
BbvCI CCTCAGC 1 cut(s) 1312
BbvI GCAGC 2 cut(s) 9, 729
BccI CCATC 6 cut(s) 134, 234, 279, 331, 461, 1050
BceAI ACGGC 2 cut(s) 498, 1334
BcgI CGANNNNNNTGC 2 cut(s) 1049, 1083
BciT130I CCWGG 1 cut(s) 381
BcnI CCSGG 2 cut(s) 234, 653
BcoDI GTCTC 1 cut(s) 646
BfaI CTAG 1 cut(s) 5
BfuAI ACCTGC 1 cut(s) 889
BisI GCNGC 4 cut(s) 23, 101, 230, 718
BlsI GCNGC 4 cut(s) 24, 102, 231, 719
Bme1390I CCNGG 3 cut(s) 234, 381, 653
Bme18I GGWCC 2 cut(s) 817, 901
BmgBI CACGTC 1 cut(s) 1009
BmgT120I GGNCC 2 cut(s) 817, 901
BmiI GGNNCC 5 cut(s) 321, 451, 818, 1039, 1114
BmrFI CCNGG 3 cut(s) 234, 381, 653
BmsI GCATC 3 cut(s) 28, 727, 1031
BpiI GAAGAC 4 cut(s) 60, 793, 825, 1255
Bpu10I CCTNAGC 1 cut(s) 1312
BpuEI CTTGAG 3 cut(s) 173, 734, 1271
BpuMI CCSGG 2 cut(s) 234, 653
BsaJI CCNNGG 4 cut(s) 95, 379, 651, 1350
BsaWI WCCGGW 4 cut(s) 248, 869, 1045, 1075
BsaXI ACNNNNNCTCC 2 cut(s) 379, 409
Bsc4I CCNNNNNNNGG 3 cut(s) 240, 359, 1224
Bse1I ACTGG 1 cut(s) 602
Bse3DI GCAATG 1 cut(s) 774
BseAI TCCGGA 1 cut(s) 1045
BseBI CCWGG 1 cut(s) 381
BseDI CCNNGG 4 cut(s) 95, 379, 651, 1350
BseGI GGATG 4 cut(s) 598, 1042, 1054, 1108
BseLI CCNNNNNNNGG 3 cut(s) 240, 359, 1224
BseMI GCAATG 1 cut(s) 774
BseMII CTCAG 5 cut(s) 17, 329, 651, 1245, 1326
BseNI ACTGG 1 cut(s) 602
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 2 cut(s) 9, 729
Bsh1285I CGRYCG 1 cut(s) 364
BshFI GGCC 2 cut(s) 67, 100
BshNI GGYRCC 1 cut(s) 1112
BsiEI CGRYCG 1 cut(s) 364
BsiHKAI GWGCWC 1 cut(s) 217
BsiSI CCGG 6 cut(s) 234, 249, 653, 870, 1046, 1076
BsiWI CGTACG 1 cut(s) 357
BslFI GGGAC 3 cut(s) 643, 665, 776
BslI CCNNNNNNNGG 3 cut(s) 240, 359, 1224
BsmAI GTCTC 1 cut(s) 646
BsmFI GGGAC 3 cut(s) 643, 665, 776
BsmI GAATGC 2 cut(s) 178, 927
BsnI GGCC 2 cut(s) 67, 100
Bsp1286I GDGCHC 1 cut(s) 217
Bsp13I TCCGGA 1 cut(s) 1045
Bsp1407I TGTACA 2 cut(s) 619, 802
Bsp143I GATC 6 cut(s) 311, 319, 467, 1060, 1084, 1146
BspACI CCGC 3 cut(s) 101, 210, 230
BspANI GGCC 2 cut(s) 67, 100
BspCNI CTCAG 5 cut(s) 18, 328, 650, 1246, 1325
BspEI TCCGGA 1 cut(s) 1045
BspLI GGNNCC 5 cut(s) 321, 451, 818, 1039, 1114
BspMI ACCTGC 1 cut(s) 889
BspPI GGATC 7 cut(s) 314, 319, 327, 475, 1055, 1092, 1154
BspQI GCTCTTC 1 cut(s) 1255
BspT107I GGYRCC 1 cut(s) 1112
BsrDI GCAATG 1 cut(s) 774
BsrGI TGTACA 2 cut(s) 619, 802
BsrI ACTGG 1 cut(s) 602
BssECI CCNNGG 4 cut(s) 95, 379, 651, 1350
BssMI GATC 6 cut(s) 311, 319, 467, 1060, 1084, 1146
BssSI CACGAG 1 cut(s) 265
BssT1I CCWWGG 1 cut(s) 95
Bst2BI CACGAG 1 cut(s) 265
Bst2UI CCWGG 1 cut(s) 381
Bst4CI ACNGT 4 cut(s) 282, 362, 461, 670
Bst6I CTCTTC 1 cut(s) 1255
BstAPI GCANNNNNTGC 1 cut(s) 1019
BstAUI TGTACA 2 cut(s) 619, 802
BstC8I GCNNGC 1 cut(s) 1318
BstDEI CTNAG 6 cut(s) 26, 315, 637, 920, 1254, 1312
BstDSI CCRYGG 1 cut(s) 1350
BstF5I GGATG 4 cut(s) 598, 1042, 1054, 1108
BstKTI GATC 6 cut(s) 314, 322, 470, 1063, 1087, 1149
BstMAI GTCTC 1 cut(s) 646
BstMBI GATC 6 cut(s) 311, 319, 467, 1060, 1084, 1146
BstMCI CGRYCG 1 cut(s) 364
BstMWI GCNNNNNNNGC 5 cut(s) 22, 226, 723, 895, 1019
BstNI CCWGG 1 cut(s) 381
BstSCI CCNGG 3 cut(s) 232, 379, 651
BstSLI GKGCMC 1 cut(s) 217
BstV1I GCAGC 2 cut(s) 9, 729
BstV2I GAAGAC 4 cut(s) 60, 793, 825, 1255
BstX2I RGATCY 2 cut(s) 311, 319
BstXI CCANNNNNNTGG 1 cut(s) 330
BstYI RGATCY 2 cut(s) 311, 319
BsuRI GGCC 2 cut(s) 67, 100
BtgI CCRYGG 1 cut(s) 1350
BtgZI GCGATG 1 cut(s) 51
BtrI CACGTC 1 cut(s) 1009
BtsCI GGATG 4 cut(s) 598, 1042, 1054, 1108
BtsIMutI CAGTG 2 cut(s) 278, 477
BveI ACCTGC 1 cut(s) 889
Cac8I GCNNGC 1 cut(s) 1318
CaiI CAGNNNCTG 1 cut(s) 644
Cfr13I GGNCC 2 cut(s) 817, 901
Csp6I GTAC 4 cut(s) 358, 620, 803, 1113
CviAII CATG 3 cut(s) 422, 727, 1275
CviQI GTAC 4 cut(s) 358, 620, 803, 1113
DdeI CTNAG 6 cut(s) 26, 315, 637, 920, 1254, 1312
DpnI GATC 6 cut(s) 313, 321, 469, 1062, 1086, 1148
DpnII GATC 6 cut(s) 311, 319, 467, 1060, 1084, 1146
DraI TTTAAA 1 cut(s) 504
DraIII CACNNNGTG 1 cut(s) 923
Eam1104I CTCTTC 1 cut(s) 1255
EarI CTCTTC 1 cut(s) 1255
Eco130I CCWWGG 1 cut(s) 95
Eco147I AGGCCT 1 cut(s) 67
Eco32I GATATC 1 cut(s) 973
Eco47I GGWCC 2 cut(s) 817, 901
EcoRI GAATTC 1 cut(s) 577
EcoRII CCWGG 1 cut(s) 379
EcoRV GATATC 1 cut(s) 973
EcoT14I CCWWGG 1 cut(s) 95
ErhI CCWWGG 1 cut(s) 95
FaeI CATG 3 cut(s) 425, 730, 1278
FaqI GGGAC 3 cut(s) 643, 665, 776
FatI CATG 3 cut(s) 421, 726, 1274
Fnu4HI GCNGC 4 cut(s) 23, 101, 230, 718
FokI GGATG 4 cut(s) 585, 1029, 1061, 1115
Fsp4HI GCNGC 4 cut(s) 23, 101, 230, 718
FspBI CTAG 1 cut(s) 5
GluI GCNGC 4 cut(s) 23, 101, 230, 718
HaeIII GGCC 2 cut(s) 67, 100
HapII CCGG 6 cut(s) 234, 249, 653, 870, 1046, 1076
Hin1II CATG 3 cut(s) 425, 730, 1278
HinfI GANTC 5 cut(s) 74, 656, 686, 1154, 1256
HpaII CCGG 6 cut(s) 234, 249, 653, 870, 1046, 1076
HphI GGTGA 2 cut(s) 149, 452
Hpy166II GTNNAC 3 cut(s) 215, 805, 904
Hpy188I TCNGA 4 cut(s) 694, 812, 985, 1255
Hpy188III TCNNGA 6 cut(s) 169, 265, 317, 341, 751, 1046
Hpy8I GTNNAC 3 cut(s) 215, 805, 904
Hpy99I CGWCG 1 cut(s) 1010
HpyAV CCTTC 2 cut(s) 1082, 1171
HpyCH4III ACNGT 4 cut(s) 282, 362, 461, 670
HpyCH4IV ACGT 1 cut(s) 1008
HpyCH4V TGCA 7 cut(s) 85, 215, 587, 612, 705, 717, 1022
HpyF10VI GCNNNNNNNGC 5 cut(s) 22, 226, 723, 895, 1019
HpyF3I CTNAG 6 cut(s) 26, 315, 637, 920, 1254, 1312
HpySE526I ACGT 1 cut(s) 1008
Hsp92II CATG 3 cut(s) 425, 730, 1278
Kpn2I TCCGGA 1 cut(s) 1045
KpnI GGTACC 1 cut(s) 1116
Kzo9I GATC 6 cut(s) 311, 319, 467, 1060, 1084, 1146
LguI GCTCTTC 1 cut(s) 1255
LmnI GCTCC 2 cut(s) 652, 895
Lsp1109I GCAGC 2 cut(s) 9, 729
LweI GCATC 3 cut(s) 28, 727, 1031
MaeI CTAG 1 cut(s) 5
MaeII ACGT 1 cut(s) 1008
MaeIII GTNAC 1 cut(s) 417
MalI GATC 6 cut(s) 313, 321, 469, 1062, 1086, 1148
MboI GATC 6 cut(s) 311, 319, 467, 1060, 1084, 1146
MflI RGATCY 2 cut(s) 311, 319
MhlI GDGCHC 1 cut(s) 217
MluCI AATT 7 cut(s) 86, 577, 978, 1136, 1201, 1219, 1303
MlyI GAGTC 2 cut(s) 665, 1265
MroI TCCGGA 1 cut(s) 1045
MseI TTAA 3 cut(s) 89, 489, 503
MslI CAYNNNNRTG 4 cut(s) 572, 725, 1007, 1047
MspI CCGG 6 cut(s) 234, 249, 653, 870, 1046, 1076
MspR9I CCNGG 3 cut(s) 234, 381, 653
Mva1269I GAATGC 2 cut(s) 178, 927
MvaI CCWGG 1 cut(s) 381
MwoI GCNNNNNNNGC 5 cut(s) 22, 226, 723, 895, 1019
NciI CCSGG 2 cut(s) 234, 653
NdeII GATC 6 cut(s) 311, 319, 467, 1060, 1084, 1146
NlaIII CATG 3 cut(s) 425, 730, 1278
NlaIV GGNNCC 5 cut(s) 321, 451, 818, 1039, 1114
OliI CACNNNNGTG 1 cut(s) 572
PceI AGGCCT 1 cut(s) 67
PciSI GCTCTTC 1 cut(s) 1255
PctI GAATGC 2 cut(s) 178, 927
PfeI GAWTC 3 cut(s) 74, 686, 1154
Pfl23II CGTACG 1 cut(s) 357
PflMI CCANNNNNTGG 1 cut(s) 1224
PkrI GCNGC 4 cut(s) 24, 102, 231, 719
PleI GAGTC 2 cut(s) 664, 1264
PpsI GAGTC 2 cut(s) 664, 1264
Psp6I CCWGG 1 cut(s) 379
PspGI CCWGG 1 cut(s) 379
PspLI CGTACG 1 cut(s) 357
PspN4I GGNNCC 5 cut(s) 321, 451, 818, 1039, 1114
PspPI GGNCC 2 cut(s) 817, 901
PstNI CAGNNNCTG 1 cut(s) 644
PsuI RGATCY 2 cut(s) 311, 319
RsaI GTAC 4 cut(s) 359, 621, 804, 1114
RsaNI GTAC 4 cut(s) 358, 620, 803, 1113
RseI CAYNNNNRTG 4 cut(s) 572, 725, 1007, 1047
SapI GCTCTTC 1 cut(s) 1255
SaqAI TTAA 3 cut(s) 89, 489, 503
SatI GCNGC 4 cut(s) 23, 101, 230, 718
Sau3AI GATC 6 cut(s) 311, 319, 467, 1060, 1084, 1146
Sau96I GGNCC 2 cut(s) 817, 901
SchI GAGTC 2 cut(s) 665, 1265
ScrFI CCNGG 3 cut(s) 234, 381, 653
SduI GDGCHC 1 cut(s) 217
SfaNI GCATC 3 cut(s) 28, 727, 1031
SinI GGWCC 2 cut(s) 817, 901
SmiMI CAYNNNNRTG 4 cut(s) 572, 725, 1007, 1047
SmlI CTYRAG 3 cut(s) 188, 749, 1286
SmoI CTYRAG 3 cut(s) 188, 749, 1286
Sse9I AATT 7 cut(s) 86, 577, 978, 1136, 1201, 1219, 1303
SseBI AGGCCT 1 cut(s) 67
SsiI CCGC 3 cut(s) 101, 210, 230
SspMI CTAG 1 cut(s) 5
StuI AGGCCT 1 cut(s) 67
StyD4I CCNGG 3 cut(s) 232, 379, 651
StyI CCWWGG 1 cut(s) 95
TaaI ACNGT 4 cut(s) 282, 362, 461, 670
TaiI ACGT 1 cut(s) 1011
TaqI TCGA 4 cut(s) 364, 1005, 1059, 1325
TasI AATT 7 cut(s) 86, 577, 978, 1136, 1201, 1219, 1303
TatI WGTACW 2 cut(s) 619, 802
TauI GCSGC 2 cut(s) 103, 232
TfiI GAWTC 3 cut(s) 74, 686, 1154
Tru1I TTAA 3 cut(s) 89, 489, 503
Tru9I TTAA 3 cut(s) 89, 489, 503
TscAI CASTG 2 cut(s) 285, 477
TseI GCWGC 2 cut(s) 22, 717
TspDTI ATGAA 1 cut(s) 587
TspRI CASTG 2 cut(s) 285, 477
Van91I CCANNNNNTGG 1 cut(s) 1224
VneI GTGCAC 1 cut(s) 213
VpaK11BI GGWCC 2 cut(s) 817, 901
XapI RAATTY 3 cut(s) 577, 1136, 1303
XspI CTAG 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.