AT3G09150

Phytochromobilin ferredoxin oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005084
Physical Location & Seq
Forward (+)
2803528 .. 2805922
2395 bp
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UTR
Exon/CDS
Intron
AT3G09150.4

Sequence Viewer

Length: 984 bp
ATGGCTTTATCAATGGAGTTTGGGTTTTCAATTGGGTCATGCTTCAAGGCACCAAACCCACCTGTTCTAATCTCTGCAAGCCCTAATAAGATCAATTTCACGTTGAGAAGGAGAAAGAAAAGATTCTTACTTAGAGTCTCTGCTGTGTCGTATAAGGAATTCGCAGAGTCTGCTTTAGAAGAAACCAGGAAAAGGATCGTTCTTGAACCTTCACATCTCCAGTATAGTAGCATGACAGGACTAGATGGTAAGACCGAACTTCAAATGCTTGCTTTTAAATCTTCAAAGATTAGACTCTTGAGGAGTATGGCAATAGAGAATGAGACAATGCAGGTCTTTGACTTTGCGGGTTTCATGGAGCCTGAGTATGATACTCCCATATTCTGTGCTAACTTTTTCACATCTACCAACGTTAACATAGTTGTATTGGACCTTAATCCTTTGCATCAGTTGACTGACCAGACGGATTACCAAGACAAGTATTATAACAAGATAATGTCCATATATCACAAATATGCTGAGACTTTCCCATGGGGAGGGAAATTGACTGGTGAATCCATAAAGTTTTTCTCGCCTTTGGTGATGTGGACTAGGTTTTCGTCTAGCAAAGAAAAACATAAGGCTTTGTTCTCTGCGTTTCTAGAGTACTATCAGGCATGGCTTGAGATGACAATCCAAGTGAGGGAGGAGATGGAACCATCTCATGTGAGAGCCAATTGTGAAGCACAACACAAGTACCTGACATGGCGAGCACAAAAGGATCCTGGACATGGTCTTCTTAAAAGATTAGTAGGTGAAGCAAAGGCAAAGGAGCTGCTAAGGGATTTCCTGTTCAATGGGGTGGATGAGTTAGGCACAAAAACATTCATTGATTACTTTCCAGAGTACCAAACAGAAGATGGAACTGTAAGCGATAAACGAAGTATCATTGGGAAGTCATATGAAACTCGTCCATGGGATTTAACAGGACAATTTATCGGCTAA

Protein Analysis

327

Amino Acids

37.87

Weight (kDa)

8.23

Isoelectric Point (pI)

43.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe_bilin_red PF05996 56 - 272 2.3e-64 Ferredoxin-dependent bilin reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 486
Acc36I ACCTGC 1 cut(s) 322
AccB1I GGYRCC 1 cut(s) 49
AciI CCGC 1 cut(s) 347
AclI AACGTT 1 cut(s) 411
AclWI GGATC 3 cut(s) 203, 755, 768
AcsI RAATTY 1 cut(s) 158
AfaI GTAC 3 cut(s) 647, 737, 887
AfiI CCNNNNNNNGG 4 cut(s) 192, 536, 682, 770
AgsI TTSAA 6 cut(s) 30, 46, 206, 263, 285, 835
AjnI CCWGG 2 cut(s) 185, 763
AloI GAACNNNNNNTCC 2 cut(s) 815, 847
AluBI AGCT 1 cut(s) 814
AluI AGCT 1 cut(s) 814
Alw21I GWGCWC 1 cut(s) 754
Alw26I GTCTC 3 cut(s) 142, 317, 515
AlwI GGATC 3 cut(s) 203, 755, 768
AlwNI CAGNNNCTG 1 cut(s) 170
ApeKI GCWGC 1 cut(s) 814
ApoI RAATTY 1 cut(s) 158
ArsI GACNNNNNNTTYG 2 cut(s) 580, 612
Asp700I GAANNNNTTC 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 430
AsuHPI GGTGA 3 cut(s) 563, 592, 806
AvaII GGWCC 1 cut(s) 430
BaeI ACNNNNGTAYC 2 cut(s) 719, 752
BamHI GGATCC 1 cut(s) 760
BanI GGYRCC 1 cut(s) 49
BbsI GAAGAC 1 cut(s) 767
Bbv12I GWGCWC 1 cut(s) 754
BbvI GCAGC 1 cut(s) 801
BccI CCATC 4 cut(s) 239, 685, 706, 893
BciT130I CCWGG 2 cut(s) 187, 765
BcoDI GTCTC 3 cut(s) 142, 317, 515
BfaI CTAG 4 cut(s) 242, 591, 603, 641
BfuAI ACCTGC 1 cut(s) 322
BisI GCNGC 1 cut(s) 815
BlsI GCNGC 1 cut(s) 816
BmcAI AGTACT 1 cut(s) 647
Bme1390I CCNGG 2 cut(s) 187, 765
Bme18I GGWCC 1 cut(s) 430
BmgT120I GGNCC 1 cut(s) 430
BmiI GGNNCC 4 cut(s) 51, 360, 696, 762
BmrFI CCNGG 2 cut(s) 187, 765
BmsI GCATC 1 cut(s) 454
BpiI GAAGAC 1 cut(s) 767
BpmI CTGGAG 1 cut(s) 203
Bpu10I CCTNAGC 1 cut(s) 818
BpuEI CTTGAG 2 cut(s) 319, 683
BsaBI GATNNNNATC 1 cut(s) 671
BsaJI CCNNGG 2 cut(s) 530, 953
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 350, 380
Bsc4I CCNNNNNNNGG 4 cut(s) 192, 536, 682, 770
Bse1I ACTGG 2 cut(s) 220, 553
Bse8I GATNNNNATC 1 cut(s) 671
BseBI CCWGG 2 cut(s) 187, 765
BseDI CCNNGG 2 cut(s) 530, 953
BseGI GGATG 1 cut(s) 850
BseJI GATNNNNATC 1 cut(s) 671
BseLI CCNNNNNNNGG 4 cut(s) 192, 536, 682, 770
BseMII CTCAG 2 cut(s) 354, 510
BseNI ACTGG 2 cut(s) 220, 553
BseRI GAGGAG 2 cut(s) 316, 701
BseXI GCAGC 1 cut(s) 801
BshNI GGYRCC 1 cut(s) 49
BsiHKAI GWGCWC 1 cut(s) 754
BslI CCNNNNNNNGG 4 cut(s) 192, 536, 682, 770
BsmAI GTCTC 3 cut(s) 142, 317, 515
Bsp1286I GDGCHC 1 cut(s) 754
Bsp143I GATC 3 cut(s) 90, 195, 760
Bsp19I CCATGG 2 cut(s) 530, 953
BspACI CCGC 1 cut(s) 347
BspCNI CTCAG 2 cut(s) 355, 511
BspLI GGNNCC 4 cut(s) 51, 360, 696, 762
BspMI ACCTGC 1 cut(s) 322
BspPI GGATC 3 cut(s) 203, 755, 768
BspT107I GGYRCC 1 cut(s) 49
BsrI ACTGG 2 cut(s) 220, 553
BssECI CCNNGG 2 cut(s) 530, 953
BssMI GATC 3 cut(s) 90, 195, 760
BssT1I CCWWGG 2 cut(s) 530, 953
Bst2UI CCWGG 2 cut(s) 187, 765
Bst4CI ACNGT 1 cut(s) 907
BstAPI GCANNNNNTGC 1 cut(s) 170
BstC8I GCNNGC 3 cut(s) 79, 270, 750
BstDEI CTNAG 4 cut(s) 131, 363, 519, 818
BstDSI CCRYGG 2 cut(s) 530, 953
BstF5I GGATG 1 cut(s) 850
BstKTI GATC 3 cut(s) 93, 198, 763
BstMAI GTCTC 3 cut(s) 142, 317, 515
BstMBI GATC 3 cut(s) 90, 195, 760
BstMWI GCNNNNNNNGC 1 cut(s) 170
BstNI CCWGG 2 cut(s) 187, 765
BstSCI CCNGG 2 cut(s) 185, 763
BstV1I GCAGC 1 cut(s) 801
BstV2I GAAGAC 1 cut(s) 767
BstX2I RGATCY 1 cut(s) 760
BstYI RGATCY 1 cut(s) 760
BtgI CCRYGG 2 cut(s) 530, 953
BtsCI GGATG 1 cut(s) 850
BveI ACCTGC 1 cut(s) 322
Cac8I GCNNGC 3 cut(s) 79, 270, 750
CaiI CAGNNNCTG 1 cut(s) 170
Cfr13I GGNCC 1 cut(s) 430
Csp6I GTAC 3 cut(s) 646, 736, 886
CviAII CATG 9 cut(s) 39, 232, 355, 531, 657, 704, 744, 770, 954
CviJI RGCY 8 cut(s) 5, 81, 361, 623, 661, 713, 814, 981
CviKI_1 RGCY 8 cut(s) 5, 81, 361, 623, 661, 713, 814, 981
CviQI GTAC 3 cut(s) 646, 736, 886
DdeI CTNAG 4 cut(s) 131, 363, 519, 818
DpnI GATC 3 cut(s) 92, 197, 762
DpnII GATC 3 cut(s) 90, 195, 760
DraI TTTAAA 1 cut(s) 277
Eco130I CCWWGG 2 cut(s) 530, 953
Eco47I GGWCC 1 cut(s) 430
EcoRI GAATTC 1 cut(s) 158
EcoRII CCWGG 2 cut(s) 185, 763
EcoT14I CCWWGG 2 cut(s) 530, 953
ErhI CCWWGG 2 cut(s) 530, 953
FaeI CATG 9 cut(s) 42, 235, 358, 534, 660, 707, 747, 773, 957
FatI CATG 9 cut(s) 38, 231, 354, 530, 656, 703, 743, 769, 953
FauI CCCGC 1 cut(s) 340
FauNDI CATATG 1 cut(s) 940
Fnu4HI GCNGC 1 cut(s) 815
FokI GGATG 1 cut(s) 857
Fsp4HI GCNGC 1 cut(s) 815
FspBI CTAG 4 cut(s) 242, 591, 603, 641
GluI GCNGC 1 cut(s) 815
GsuI CTGGAG 1 cut(s) 203
Hin1II CATG 9 cut(s) 42, 235, 358, 534, 660, 707, 747, 773, 957
HincII GTYRAC 2 cut(s) 415, 453
HindII GTYRAC 2 cut(s) 415, 453
HinfI GANTC 5 cut(s) 123, 135, 167, 294, 554
HpaI GTTAAC 1 cut(s) 415
HphI GGTGA 3 cut(s) 563, 592, 806
Hpy166II GTNNAC 3 cut(s) 415, 453, 588
Hpy188III TCNNGA 4 cut(s) 203, 298, 641, 881
Hpy8I GTNNAC 3 cut(s) 415, 453, 588
HpyAV CCTTC 2 cut(s) 102, 219
HpyCH4III ACNGT 1 cut(s) 907
HpyCH4IV ACGT 2 cut(s) 101, 411
HpyCH4V TGCA 3 cut(s) 77, 331, 445
HpyF10VI GCNNNNNNNGC 1 cut(s) 170
HpyF3I CTNAG 4 cut(s) 131, 363, 519, 818
HpySE526I ACGT 2 cut(s) 101, 411
Hsp92II CATG 9 cut(s) 42, 235, 358, 534, 660, 707, 747, 773, 957
KspAI GTTAAC 1 cut(s) 415
Kzo9I GATC 3 cut(s) 90, 195, 760
LmnI GCTCC 2 cut(s) 358, 811
Lsp1109I GCAGC 1 cut(s) 801
LweI GCATC 1 cut(s) 454
MaeI CTAG 4 cut(s) 242, 591, 603, 641
MaeII ACGT 2 cut(s) 101, 411
MalI GATC 3 cut(s) 92, 197, 762
MboI GATC 3 cut(s) 90, 195, 760
MboII GAAGA 4 cut(s) 191, 273, 767, 908
MfeI CAATTG 2 cut(s) 30, 715
MflI RGATCY 1 cut(s) 760
MhlI GDGCHC 1 cut(s) 754
MluCI AATT 6 cut(s) 30, 94, 158, 542, 715, 971
MlyI GAGTC 3 cut(s) 144, 176, 288
MnlI CCTC 4 cut(s) 294, 530, 675, 679
MroXI GAANNNNTTC 1 cut(s) 122
MseI TTAA 5 cut(s) 276, 414, 435, 780, 962
MslI CAYNNNNRTG 1 cut(s) 513
MspR9I CCNGG 2 cut(s) 187, 765
MunI CAATTG 2 cut(s) 30, 715
MvaI CCWGG 2 cut(s) 187, 765
MwoI GCNNNNNNNGC 1 cut(s) 170
NcoI CCATGG 2 cut(s) 530, 953
NdeI CATATG 1 cut(s) 940
NdeII GATC 3 cut(s) 90, 195, 760
NlaIII CATG 9 cut(s) 42, 235, 358, 534, 660, 707, 747, 773, 957
NlaIV GGNNCC 4 cut(s) 51, 360, 696, 762
PdmI GAANNNNTTC 1 cut(s) 122
PfeI GAWTC 2 cut(s) 123, 554
PflFI GACNNNGTC 1 cut(s) 771
PfoI TCCNGGA 1 cut(s) 763
PkrI GCNGC 1 cut(s) 816
PleI GAGTC 3 cut(s) 143, 175, 288
PpsI GAGTC 3 cut(s) 143, 175, 288
PsiI TTATAA 1 cut(s) 486
Psp1406I AACGTT 1 cut(s) 411
Psp6I CCWGG 2 cut(s) 185, 763
PspGI CCWGG 2 cut(s) 185, 763
PspN4I GGNNCC 4 cut(s) 51, 360, 696, 762
PspPI GGNCC 1 cut(s) 430
PstNI CAGNNNCTG 1 cut(s) 170
PsuI RGATCY 1 cut(s) 760
PsyI GACNNNGTC 1 cut(s) 771
RsaI GTAC 3 cut(s) 647, 737, 887
RsaNI GTAC 3 cut(s) 646, 736, 886
RseI CAYNNNNRTG 1 cut(s) 513
SaqAI TTAA 5 cut(s) 276, 414, 435, 780, 962
SatI GCNGC 1 cut(s) 815
Sau3AI GATC 3 cut(s) 90, 195, 760
Sau96I GGNCC 1 cut(s) 430
ScaI AGTACT 1 cut(s) 647
SchI GAGTC 3 cut(s) 144, 176, 288
ScrFI CCNGG 2 cut(s) 187, 765
SduI GDGCHC 1 cut(s) 754
SfaNI GCATC 1 cut(s) 454
SinI GGWCC 1 cut(s) 430
SmiMI CAYNNNNRTG 1 cut(s) 513
SmlI CTYRAG 2 cut(s) 298, 662
SmoI CTYRAG 2 cut(s) 298, 662
Sse9I AATT 6 cut(s) 30, 94, 158, 542, 715, 971
SsiI CCGC 1 cut(s) 347
SspMI CTAG 4 cut(s) 242, 591, 603, 641
StyD4I CCNGG 2 cut(s) 185, 763
StyI CCWWGG 2 cut(s) 530, 953
TaaI ACNGT 1 cut(s) 907
TaiI ACGT 2 cut(s) 104, 414
TaqII GACCGA 1 cut(s) 269
TasI AATT 6 cut(s) 30, 94, 158, 542, 715, 971
TatI WGTACW 1 cut(s) 645
TfiI GAWTC 2 cut(s) 123, 554
Tru1I TTAA 5 cut(s) 276, 414, 435, 780, 962
Tru9I TTAA 5 cut(s) 276, 414, 435, 780, 962
TseI GCWGC 1 cut(s) 814
TspDTI ATGAA 3 cut(s) 343, 856, 957
TspGWI ACGGA 1 cut(s) 479
Tth111I GACNNNGTC 1 cut(s) 771
VpaK11BI GGWCC 1 cut(s) 430
XapI RAATTY 1 cut(s) 158
XbaI TCTAGA 1 cut(s) 640
XcmI CCANNNNNNNNNTGG 1 cut(s) 896
XmnI GAANNNNTTC 1 cut(s) 122
XspI CTAG 4 cut(s) 242, 591, 603, 641
ZrmI AGTACT 1 cut(s) 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.