MD03G1169100.v1.1

Phytochromobilin ferredoxin oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005084
Physical Location & Seq
Forward (+)
22887909 .. 22891156
3248 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1169100.v1.1.491

Sequence Viewer

Length: 756 bp
ATGGATGGTAAAGCAGATATTCAAATGTTATCATTTGAAGCTACTAAAATTAGACATCTACGTAGCTTATGCATTGAGAGCCAAACAATGCAGATTTTGGATTTTGCTGTCTTCCCAGAACCCGAATTTGATATGCCCATATTTTGTGCCAACTTCTTCACCAGTGCAAATATAAACATAGTTGTGCTGGACCTCAATCCGTTGCATGATGTAATCAGTCAAAGGCATTACAGAGACAAATACTATAAAAGCCTAATACCTCTTGGTGTTAAATATTCTGAGCTTTTACCCTGGGGAGGAAAGCTTACGAGTGAGTCACTTAAATTCTTTTCACCAATTGTGATATGGACAAGATTTACGTCAAGCCCCAACAATTATGATGTTTTATATTCTGCATTCATGGATTATTACAAGGCATGGCTTGCACTGATGGACCAAGCAGTAGTGGAGACAAATGCCTCCCAAATTATATGTAACCGTGAAGCGCAACATAGATATCTAACATGGAGAGCAGAGAAGGATCCCGGACATGCACTACTGAAAAAGTTGATTGGGGAGACACAAGCTAAGGACTTGCTGGTGAACTTCCTCTTTAATGGAATCGATGAACTAGGAAGCAAAACTTTCCTCGATTACTTTCCAGAGTACGGCTGTGAGGATGGGACTGTAAACCAGAGTCGCAGTATGATAGGGAAATCTTTTGAAAGTCGCCCCTGGGACACAAAAGGAGAATTTATTAGTAACACACACTTTTGA

Protein Analysis

252

Amino Acids

28.98

Weight (kDa)

5.54

Isoelectric Point (pI)

35.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe_bilin_red PF05996 5 - 199 1.7e-52 Ferredoxin-dependent bilin reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 515, 528
AcsI RAATTY 3 cut(s) 125, 323, 731
AfaI GTAC 1 cut(s) 647
AfiI CCNNNNNNNGG 2 cut(s) 296, 647
AgsI TTSAA 3 cut(s) 23, 38, 704
AjnI CCWGG 2 cut(s) 290, 713
AluBI AGCT 5 cut(s) 41, 66, 283, 304, 566
AluI AGCT 5 cut(s) 41, 66, 283, 304, 566
Alw26I GTCTC 3 cut(s) 228, 443, 551
AlwI GGATC 2 cut(s) 515, 528
ApoI RAATTY 3 cut(s) 125, 323, 731
AspLEI GCGC 1 cut(s) 487
AspS9I GGNCC 2 cut(s) 190, 433
AsuC2I CCSGG 1 cut(s) 525
AsuHPI GGTGA 3 cut(s) 151, 324, 592
AvaII GGWCC 2 cut(s) 190, 433
BamHI GGATCC 1 cut(s) 520
BbsI GAAGAC 1 cut(s) 103
BccI CCATC 2 cut(s) 424, 653
BceAI ACGGC 1 cut(s) 664
BciT130I CCWGG 2 cut(s) 292, 715
BcnI CCSGG 1 cut(s) 525
BcoDI GTCTC 3 cut(s) 228, 443, 551
BfaI CTAG 1 cut(s) 611
Bme1390I CCNGG 3 cut(s) 292, 525, 715
Bme18I GGWCC 2 cut(s) 190, 433
BmgT120I GGNCC 2 cut(s) 190, 433
BmiI GGNNCC 1 cut(s) 522
BmrFI CCNGG 3 cut(s) 292, 525, 715
BpiI GAAGAC 1 cut(s) 103
Bpu10I CCTNAGC 1 cut(s) 567
BpuMI CCSGG 1 cut(s) 525
Bsa29I ATCGAT 1 cut(s) 603
BsaAI YACGTR 1 cut(s) 62
BsaJI CCNNGG 4 cut(s) 290, 291, 713, 714
Bsc4I CCNNNNNNNGG 2 cut(s) 296, 647
Bse1I ACTGG 1 cut(s) 162
BseBI CCWGG 2 cut(s) 292, 715
BseCI ATCGAT 1 cut(s) 603
BseDI CCNNGG 4 cut(s) 290, 291, 713, 714
BseGI GGATG 2 cut(s) 10, 664
BseLI CCNNNNNNNGG 2 cut(s) 296, 647
BseMII CTCAG 1 cut(s) 270
BseNI ACTGG 1 cut(s) 162
BshVI ATCGAT 1 cut(s) 603
BsiSI CCGG 1 cut(s) 525
BslFI GGGAC 2 cut(s) 676, 731
BslI CCNNNNNNNGG 2 cut(s) 296, 647
BsmAI GTCTC 3 cut(s) 228, 443, 551
BsmFI GGGAC 2 cut(s) 676, 731
BsmI GAATGC 1 cut(s) 395
Bsp143I GATC 1 cut(s) 520
BspCNI CTCAG 1 cut(s) 271
BspDI ATCGAT 1 cut(s) 603
BspLI GGNNCC 1 cut(s) 522
BspPI GGATC 2 cut(s) 515, 528
BsrI ACTGG 1 cut(s) 162
BssECI CCNNGG 4 cut(s) 290, 291, 713, 714
BssMI GATC 1 cut(s) 520
Bst2UI CCWGG 2 cut(s) 292, 715
Bst4CI ACNGT 2 cut(s) 479, 667
BstAPI GCANNNNNTGC 1 cut(s) 422
BstBAI YACGTR 1 cut(s) 62
BstC8I GCNNGC 1 cut(s) 423
BstDEI CTNAG 2 cut(s) 279, 567
BstF5I GGATG 2 cut(s) 10, 664
BstHHI GCGC 1 cut(s) 487
BstKTI GATC 1 cut(s) 523
BstMAI GTCTC 3 cut(s) 228, 443, 551
BstMBI GATC 1 cut(s) 520
BstMWI GCNNNNNNNGC 2 cut(s) 78, 422
BstNI CCWGG 2 cut(s) 292, 715
BstNSI RCATGY 1 cut(s) 533
BstSCI CCNGG 3 cut(s) 290, 523, 713
BstSNI TACGTA 1 cut(s) 62
BstV2I GAAGAC 1 cut(s) 103
BstX2I RGATCY 1 cut(s) 520
BstYI RGATCY 1 cut(s) 520
Bsu15I ATCGAT 1 cut(s) 603
BsuTUI ATCGAT 1 cut(s) 603
BtsCI GGATG 2 cut(s) 10, 664
BtsIMutI CAGTG 2 cut(s) 169, 425
Cac8I GCNNGC 1 cut(s) 423
CfoI GCGC 1 cut(s) 487
Cfr13I GGNCC 2 cut(s) 190, 433
ClaI ATCGAT 1 cut(s) 603
Csp6I GTAC 1 cut(s) 646
CviAII CATG 5 cut(s) 206, 400, 417, 504, 530
CviQI GTAC 1 cut(s) 646
DdeI CTNAG 2 cut(s) 279, 567
DpnI GATC 1 cut(s) 522
DpnII GATC 1 cut(s) 520
Eco105I TACGTA 1 cut(s) 62
Eco32I GATATC 1 cut(s) 497
Eco47I GGWCC 2 cut(s) 190, 433
EcoRII CCWGG 2 cut(s) 290, 713
EcoRV GATATC 1 cut(s) 497
EcoT22I ATGCAT 1 cut(s) 74
FaeI CATG 5 cut(s) 209, 403, 420, 507, 533
FalI AAGNNNNNCTT 2 cut(s) 607, 639
FaqI GGGAC 2 cut(s) 676, 731
FatI CATG 5 cut(s) 205, 399, 416, 503, 529
FokI GGATG 2 cut(s) 17, 671
FspBI CTAG 1 cut(s) 611
GlaI GCGC 1 cut(s) 486
HapII CCGG 1 cut(s) 525
HhaI GCGC 1 cut(s) 487
Hin1II CATG 5 cut(s) 209, 403, 420, 507, 533
Hin6I GCGC 1 cut(s) 485
HinP1I GCGC 1 cut(s) 485
HindIII AAGCTT 1 cut(s) 302
HinfI GANTC 3 cut(s) 314, 600, 676
HpaII CCGG 1 cut(s) 525
HphI GGTGA 3 cut(s) 151, 324, 592
Hpy166II GTNNAC 2 cut(s) 583, 670
Hpy188I TCNGA 1 cut(s) 280
Hpy188III TCNNGA 1 cut(s) 641
Hpy8I GTNNAC 2 cut(s) 583, 670
HpyAV CCTTC 1 cut(s) 511
HpyCH4III ACNGT 2 cut(s) 479, 667
HpyCH4IV ACGT 2 cut(s) 61, 359
HpyCH4V TGCA 7 cut(s) 72, 91, 167, 205, 395, 425, 533
HpyF10VI GCNNNNNNNGC 2 cut(s) 78, 422
HpyF3I CTNAG 2 cut(s) 279, 567
HpySE526I ACGT 2 cut(s) 61, 359
Hsp92II CATG 5 cut(s) 209, 403, 420, 507, 533
HspAI GCGC 1 cut(s) 485
Kzo9I GATC 1 cut(s) 520
MaeI CTAG 1 cut(s) 611
MaeII ACGT 2 cut(s) 61, 359
MaeIII GTNAC 3 cut(s) 315, 473, 740
MalI GATC 1 cut(s) 522
MboI GATC 1 cut(s) 520
MboII GAAGA 2 cut(s) 103, 148
MfeI CAATTG 1 cut(s) 336
MflI RGATCY 1 cut(s) 520
MluCI AATT 7 cut(s) 48, 125, 323, 336, 373, 465, 731
MlyI GAGTC 2 cut(s) 323, 685
MnlI CCTC 7 cut(s) 203, 270, 290, 469, 599, 638, 649
Mph1103I ATGCAT 1 cut(s) 74
MseI TTAA 3 cut(s) 270, 321, 594
MslI CAYNNNNRTG 1 cut(s) 182
MspI CCGG 1 cut(s) 525
MspR9I CCNGG 3 cut(s) 292, 525, 715
MunI CAATTG 1 cut(s) 336
Mva1269I GAATGC 1 cut(s) 395
MvaI CCWGG 2 cut(s) 292, 715
MwoI GCNNNNNNNGC 2 cut(s) 78, 422
NciI CCSGG 1 cut(s) 525
NdeII GATC 1 cut(s) 520
NlaIII CATG 5 cut(s) 209, 403, 420, 507, 533
NlaIV GGNNCC 1 cut(s) 522
NmuCI GTSAC 1 cut(s) 315
NsiI ATGCAT 1 cut(s) 74
NspI RCATGY 1 cut(s) 533
PasI CCCWGGG 2 cut(s) 291, 714
PctI GAATGC 1 cut(s) 395
PfeI GAWTC 1 cut(s) 600
PfoI TCCNGGA 1 cut(s) 523
PleI GAGTC 2 cut(s) 322, 684
PpsI GAGTC 2 cut(s) 322, 684
Ppu21I YACGTR 1 cut(s) 62
Psp6I CCWGG 2 cut(s) 290, 713
PspGI CCWGG 2 cut(s) 290, 713
PspN4I GGNNCC 1 cut(s) 522
PspPI GGNCC 2 cut(s) 190, 433
PsuI RGATCY 1 cut(s) 520
RsaI GTAC 1 cut(s) 647
RsaNI GTAC 1 cut(s) 646
RseI CAYNNNNRTG 1 cut(s) 182
SaqAI TTAA 3 cut(s) 270, 321, 594
Sau3AI GATC 1 cut(s) 520
Sau96I GGNCC 2 cut(s) 190, 433
SchI GAGTC 2 cut(s) 323, 685
ScrFI CCNGG 3 cut(s) 292, 525, 715
SetI ASST 9 cut(s) 43, 64, 68, 195, 262, 285, 306, 362, 568
SinI GGWCC 2 cut(s) 190, 433
SmiMI CAYNNNNRTG 1 cut(s) 182
SnaBI TACGTA 1 cut(s) 62
Sse9I AATT 7 cut(s) 48, 125, 323, 336, 373, 465, 731
SspI AATATT 1 cut(s) 275
SspMI CTAG 1 cut(s) 611
StyD4I CCNGG 3 cut(s) 290, 523, 713
TaaI ACNGT 2 cut(s) 479, 667
TaiI ACGT 2 cut(s) 64, 362
TaqI TCGA 2 cut(s) 603, 630
TasI AATT 7 cut(s) 48, 125, 323, 336, 373, 465, 731
TfiI GAWTC 1 cut(s) 600
Tru1I TTAA 3 cut(s) 270, 321, 594
Tru9I TTAA 3 cut(s) 270, 321, 594
TscAI CASTG 2 cut(s) 169, 432
TseFI GTSAC 1 cut(s) 315
Tsp45I GTSAC 1 cut(s) 315
TspDTI ATGAA 2 cut(s) 388, 621
TspGWI ACGGA 1 cut(s) 189
TspRI CASTG 2 cut(s) 169, 432
VpaK11BI GGWCC 2 cut(s) 190, 433
XapI RAATTY 3 cut(s) 125, 323, 731
XceI RCATGY 1 cut(s) 533
XcmI CCANNNNNNNNNTGG 1 cut(s) 342
XspI CTAG 1 cut(s) 611
Zsp2I ATGCAT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.