Rroxscaffold_1G00029890

Phytochromobilin ferredoxin oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005084
Physical Location & Seq
Reverse (-)
38869440 .. 38875481
6042 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00029890.1

Sequence Viewer

Length: 1110 bp
ATGGAATGCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTCCTTCCTCTTCTTTAAGCAGCTTTTCCATACTGCGAAAGCCTTGCCCATTTGTACCACCATTTACCACCGTTTATAGGAAGCAGAAGCAGCAGCAGCAGCGGCGACCCCTTATCCAACTCTCCACAATTTCTTACAACAAATTCATTCACTTCGCCTTGGATGAATCCAAACGCCGCACCCATTTGGCCCCTTCCCCCTCCAGGTGTTACTTTGCCTGGCCTAGTGGGTTTTCTGGTTGTCCTTGTTCGTGGTTCCTGATGGACTTCTTCTCCACCTTGCTTTCTACTCAAAGGTATAATTCCATGGTTTCCATGGATGGTAATGCAGAACTTCAAATGTCATCATTCAAAGCTACTAAGATCAGGCTTTTACGTAGCCTATGTATTGAGACTCAAACAATGCAGGTTTTGGATTTTGCTGTCTTCCCAGAACCAGAATTTGATATGCCAATATTTTGTGCCAACTTCTTCACCAGTGCGGATGTCAACATAGTTGTGCTGGACCTCAACCCTTTGCACAACGTTATTATTGAAAGAGAATACAGAGATAAATACTACAAAGGCCTAATACCTCTTGGTGTCAAATATGCTGAGCTTTTACCGTGGGGTGGGAAGCTTACTAGCGAGTCATTGAAATTTTTTTCACCCATTGTTATATGGACAAGATTTACGTCAAGTCCACAAAAATATGAGGTTTTGTATTCGGCTTTCATGGATTATTACAAGGCATGGCTCGAGCTGATGGACCAAGCAGTAGTGGAGACAAATGCCTCTCAAATTAAACGTAACCGTGAAGCGCAACATCGATATCTAACTTGGAGAGCAGAAAAGGATCCTGGACATGGAGTTCTGAAAAGGTTGATTGGGGAGACACAAGCAAAGGATTTGCTGGTGAACTTCCTGTTCAATGGAATTGATAAACTAGGAAGCAAAAGTTTCCTGGAGTACTTTCCCGAGTACTGCTGTGAGGATGGGACTATAAATCAGAAACGTAGTATAATTGGAAAGTCTTATGAAAGTCGCCCGTGGGATAGAAAAGGAGAGTTTGTTAGTAGCAATTTTGAAAGTTAG

Protein Analysis

369

Amino Acids

42.56

Weight (kDa)

8.82

Isoelectric Point (pI)

50.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe_bilin_red PF05996 111 - 316 2.7e-54 Ferredoxin-dependent bilin reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 433
AciI CCGC 3 cut(s) 139, 214, 518
AclI AACGTT 1 cut(s) 561
AclWI GGATC 2 cut(s) 866, 879
AcsI RAATTY 3 cut(s) 179, 476, 674
AfaI GTAC 3 cut(s) 93, 986, 998
AfiI CCNNNNNNNGG 4 cut(s) 114, 240, 647, 881
AgsI TTSAA 6 cut(s) 374, 388, 572, 673, 946, 1103
AjnI CCWGG 4 cut(s) 239, 254, 874, 978
AloI GAACNNNNNNTCC 2 cut(s) 870, 902
AluBI AGCT 5 cut(s) 60, 392, 634, 655, 778
AluI AGCT 5 cut(s) 60, 392, 634, 655, 778
Alw26I GTCTC 3 cut(s) 422, 794, 902
AlwI GGATC 2 cut(s) 866, 879
Ama87I CYCGRG 2 cut(s) 773, 992
AoxI GGCC 3 cut(s) 225, 257, 601
ApeKI GCWGC 5 cut(s) 57, 127, 130, 133, 136
ApoI RAATTY 3 cut(s) 179, 476, 674
AspLEI GCGC 1 cut(s) 838
AspS9I GGNCC 3 cut(s) 226, 541, 784
AsuHPI GGTGA 3 cut(s) 502, 675, 943
AvaI CYCGRG 2 cut(s) 773, 992
AvaII GGWCC 2 cut(s) 541, 784
BamHI GGATCC 1 cut(s) 871
BbsI GAAGAC 1 cut(s) 454
BbvI GCAGC 5 cut(s) 69, 139, 142, 145, 148
BccI CCATC 4 cut(s) 292, 350, 775, 1004
BcgI CGANNNNNNTGC 2 cut(s) 63, 97
BciT130I CCWGG 4 cut(s) 241, 256, 876, 980
BcoDI GTCTC 3 cut(s) 422, 794, 902
BfaI CTAG 3 cut(s) 261, 660, 962
BfuAI ACCTGC 1 cut(s) 433
BisI GCNGC 7 cut(s) 58, 128, 131, 134, 137, 140, 214
BlpI GCTNAGC 1 cut(s) 630
BlsI GCNGC 7 cut(s) 59, 129, 132, 135, 138, 141, 215
BmcAI AGTACT 2 cut(s) 986, 998
Bme1390I CCNGG 4 cut(s) 241, 256, 876, 980
Bme18I GGWCC 2 cut(s) 541, 784
BmeT110I CYCGRG 2 cut(s) 773, 992
BmgT120I GGNCC 3 cut(s) 226, 541, 784
BmiI GGNNCC 3 cut(s) 228, 293, 873
BmrFI CCNGG 4 cut(s) 241, 256, 876, 980
BpiI GAAGAC 1 cut(s) 454
BpmI CTGGAG 2 cut(s) 223, 1001
Bpu1102I GCTNAGC 1 cut(s) 630
Bsa29I ATCGAT 1 cut(s) 844
BsaAI YACGTR 1 cut(s) 413
BsaJI CCNNGG 5 cut(s) 195, 342, 351, 641, 1064
BsaXI ACNNNNNCTCC 4 cut(s) 293, 323, 1071, 1101
Bsc4I CCNNNNNNNGG 4 cut(s) 114, 240, 647, 881
Bse1I ACTGG 1 cut(s) 513
BseBI CCWGG 4 cut(s) 241, 256, 876, 980
BseCI ATCGAT 1 cut(s) 844
BseDI CCNNGG 5 cut(s) 195, 342, 351, 641, 1064
BseGI GGATG 4 cut(s) 205, 361, 526, 1015
BseLI CCNNNNNNNGG 4 cut(s) 114, 240, 647, 881
BseMII CTCAG 1 cut(s) 621
BseNI ACTGG 1 cut(s) 513
BseXI GCAGC 5 cut(s) 69, 139, 142, 145, 148
BshFI GGCC 3 cut(s) 227, 259, 603
BshVI ATCGAT 1 cut(s) 844
BsiHKCI CYCGRG 2 cut(s) 773, 992
BslFI GGGAC 1 cut(s) 1027
BslI CCNNNNNNNGG 4 cut(s) 114, 240, 647, 881
BsmAI GTCTC 3 cut(s) 422, 794, 902
BsmFI GGGAC 1 cut(s) 1027
BsmI GAATGC 1 cut(s) 11
BsnI GGCC 3 cut(s) 227, 259, 603
BsoBI CYCGRG 2 cut(s) 773, 992
Bsp143I GATC 2 cut(s) 399, 871
Bsp1720I GCTNAGC 1 cut(s) 630
Bsp19I CCATGG 2 cut(s) 342, 351
BspACI CCGC 3 cut(s) 139, 214, 518
BspANI GGCC 3 cut(s) 227, 259, 603
BspCNI CTCAG 1 cut(s) 622
BspDI ATCGAT 1 cut(s) 844
BspLI GGNNCC 3 cut(s) 228, 293, 873
BspMI ACCTGC 1 cut(s) 433
BspPI GGATC 2 cut(s) 866, 879
BspQI GCTCTTC 1 cut(s) 16
BsrI ACTGG 1 cut(s) 513
BssECI CCNNGG 5 cut(s) 195, 342, 351, 641, 1064
BssMI GATC 2 cut(s) 399, 871
BssT1I CCWWGG 3 cut(s) 195, 342, 351
Bst2UI CCWGG 4 cut(s) 241, 256, 876, 980
Bst4CI ACNGT 3 cut(s) 109, 642, 830
Bst6I CTCTTC 2 cut(s) 16, 52
BstBAI YACGTR 1 cut(s) 413
BstDEI CTNAG 2 cut(s) 396, 630
BstDSI CCRYGG 4 cut(s) 342, 351, 641, 1064
BstF5I GGATG 4 cut(s) 205, 361, 526, 1015
BstHHI GCGC 1 cut(s) 838
BstKTI GATC 2 cut(s) 402, 874
BstMAI GTCTC 3 cut(s) 422, 794, 902
BstMBI GATC 2 cut(s) 399, 871
BstMWI GCNNNNNNNGC 4 cut(s) 127, 133, 136, 139
BstNI CCWGG 4 cut(s) 241, 256, 876, 980
BstSCI CCNGG 4 cut(s) 239, 254, 874, 978
BstSNI TACGTA 1 cut(s) 413
BstV1I GCAGC 5 cut(s) 69, 139, 142, 145, 148
BstV2I GAAGAC 1 cut(s) 454
BstX2I RGATCY 1 cut(s) 871
BstYI RGATCY 1 cut(s) 871
Bsu15I ATCGAT 1 cut(s) 844
BsuRI GGCC 3 cut(s) 227, 259, 603
BsuTUI ATCGAT 1 cut(s) 844
BtgI CCRYGG 4 cut(s) 342, 351, 641, 1064
BtsCI GGATG 4 cut(s) 205, 361, 526, 1015
BtsIMutI CAGTG 1 cut(s) 520
BveI ACCTGC 1 cut(s) 433
CfoI GCGC 1 cut(s) 838
Cfr13I GGNCC 3 cut(s) 226, 541, 784
ClaI ATCGAT 1 cut(s) 844
Csp6I GTAC 3 cut(s) 92, 985, 997
CviAII CATG 5 cut(s) 343, 352, 751, 768, 881
CviQI GTAC 3 cut(s) 92, 985, 997
DdeI CTNAG 2 cut(s) 396, 630
DpnI GATC 2 cut(s) 401, 873
DpnII GATC 2 cut(s) 399, 871
Eam1104I CTCTTC 2 cut(s) 16, 52
EarI CTCTTC 2 cut(s) 16, 52
Eco105I TACGTA 1 cut(s) 413
Eco130I CCWWGG 3 cut(s) 195, 342, 351
Eco147I AGGCCT 1 cut(s) 603
Eco32I GATATC 1 cut(s) 848
Eco47I GGWCC 2 cut(s) 541, 784
Eco88I CYCGRG 2 cut(s) 773, 992
EcoRII CCWGG 4 cut(s) 239, 254, 874, 978
EcoRV GATATC 1 cut(s) 848
EcoT14I CCWWGG 3 cut(s) 195, 342, 351
ErhI CCWWGG 3 cut(s) 195, 342, 351
FaeI CATG 5 cut(s) 346, 355, 754, 771, 884
FaqI GGGAC 1 cut(s) 1027
FatI CATG 5 cut(s) 342, 351, 750, 767, 880
Fnu4HI GCNGC 7 cut(s) 58, 128, 131, 134, 137, 140, 214
FokI GGATG 4 cut(s) 212, 368, 533, 1022
Fsp4HI GCNGC 7 cut(s) 58, 128, 131, 134, 137, 140, 214
FspBI CTAG 3 cut(s) 261, 660, 962
GlaI GCGC 1 cut(s) 837
GluI GCNGC 7 cut(s) 58, 128, 131, 134, 137, 140, 214
GsuI CTGGAG 2 cut(s) 223, 1001
HaeIII GGCC 3 cut(s) 227, 259, 603
HhaI GCGC 1 cut(s) 838
Hin1II CATG 5 cut(s) 346, 355, 754, 771, 884
Hin6I GCGC 1 cut(s) 836
HinP1I GCGC 1 cut(s) 836
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HindIII AAGCTT 1 cut(s) 653
HinfI GANTC 3 cut(s) 203, 430, 665
HphI GGTGA 3 cut(s) 502, 675, 943
Hpy166II GTNNAC 3 cut(s) 526, 719, 934
Hpy188I TCNGA 2 cut(s) 891, 1026
Hpy188III TCNNGA 2 cut(s) 295, 992
Hpy8I GTNNAC 3 cut(s) 526, 719, 934
HpyAV CCTTC 2 cut(s) 51, 240
HpyCH4III ACNGT 3 cut(s) 109, 642, 830
HpyCH4IV ACGT 5 cut(s) 412, 561, 710, 823, 1030
HpyCH4V TGCA 3 cut(s) 365, 442, 556
HpyF10VI GCNNNNNNNGC 4 cut(s) 127, 133, 136, 139
HpyF3I CTNAG 2 cut(s) 396, 630
HpySE526I ACGT 5 cut(s) 412, 561, 710, 823, 1030
Hsp92II CATG 5 cut(s) 346, 355, 754, 771, 884
HspAI GCGC 1 cut(s) 836
Kzo9I GATC 2 cut(s) 399, 871
LguI GCTCTTC 1 cut(s) 16
Lsp1109I GCAGC 5 cut(s) 69, 139, 142, 145, 148
MaeI CTAG 3 cut(s) 261, 660, 962
MaeII ACGT 5 cut(s) 412, 561, 710, 823, 1030
MaeIII GTNAC 2 cut(s) 245, 824
MalI GATC 2 cut(s) 401, 873
MboI GATC 2 cut(s) 399, 871
MflI RGATCY 1 cut(s) 871
MluCI AATT 9 cut(s) 165, 179, 337, 476, 674, 816, 951, 1038, 1096
MlyI GAGTC 2 cut(s) 424, 674
MmeI TCCRAC 1 cut(s) 178
MnlI CCTC 7 cut(s) 55, 247, 554, 621, 724, 820, 1000
MseI TTAA 2 cut(s) 53, 819
MslI CAYNNNNRTG 1 cut(s) 533
MspA1I CMGCKG 1 cut(s) 139
MspR9I CCNGG 4 cut(s) 241, 256, 876, 980
Mva1269I GAATGC 1 cut(s) 11
MvaI CCWGG 4 cut(s) 241, 256, 876, 980
MwoI GCNNNNNNNGC 4 cut(s) 127, 133, 136, 139
NcoI CCATGG 2 cut(s) 342, 351
NdeII GATC 2 cut(s) 399, 871
NlaIII CATG 5 cut(s) 346, 355, 754, 771, 884
NlaIV GGNNCC 3 cut(s) 228, 293, 873
PaeR7I CTCGAG 1 cut(s) 773
PceI AGGCCT 1 cut(s) 603
PciSI GCTCTTC 1 cut(s) 16
PctI GAATGC 1 cut(s) 11
PfeI GAWTC 1 cut(s) 203
PfoI TCCNGGA 2 cut(s) 874, 978
PkrI GCNGC 7 cut(s) 59, 129, 132, 135, 138, 141, 215
PleI GAGTC 2 cut(s) 424, 673
PpsI GAGTC 2 cut(s) 424, 673
Ppu21I YACGTR 1 cut(s) 413
Psp1406I AACGTT 1 cut(s) 561
Psp6I CCWGG 4 cut(s) 239, 254, 874, 978
PspGI CCWGG 4 cut(s) 239, 254, 874, 978
PspN4I GGNNCC 3 cut(s) 228, 293, 873
PspPI GGNCC 3 cut(s) 226, 541, 784
PspXI VCTCGAGB 1 cut(s) 773
PsuI RGATCY 1 cut(s) 871
RsaI GTAC 3 cut(s) 93, 986, 998
RsaNI GTAC 3 cut(s) 92, 985, 997
RseI CAYNNNNRTG 1 cut(s) 533
SapI GCTCTTC 1 cut(s) 16
SaqAI TTAA 2 cut(s) 53, 819
SatI GCNGC 7 cut(s) 58, 128, 131, 134, 137, 140, 214
Sau3AI GATC 2 cut(s) 399, 871
Sau96I GGNCC 3 cut(s) 226, 541, 784
ScaI AGTACT 2 cut(s) 986, 998
SchI GAGTC 2 cut(s) 424, 674
ScrFI CCNGG 4 cut(s) 241, 256, 876, 980
Sfr274I CTCGAG 1 cut(s) 773
SinI GGWCC 2 cut(s) 541, 784
SlaI CTCGAG 1 cut(s) 773
SmiMI CAYNNNNRTG 1 cut(s) 533
SmlI CTYRAG 1 cut(s) 773
SmoI CTYRAG 1 cut(s) 773
SnaBI TACGTA 1 cut(s) 413
Sse9I AATT 9 cut(s) 165, 179, 337, 476, 674, 816, 951, 1038, 1096
SseBI AGGCCT 1 cut(s) 603
SsiI CCGC 3 cut(s) 139, 214, 518
SspI AATATT 1 cut(s) 492
SspMI CTAG 3 cut(s) 261, 660, 962
StuI AGGCCT 1 cut(s) 603
StyD4I CCNGG 4 cut(s) 239, 254, 874, 978
StyI CCWWGG 3 cut(s) 195, 342, 351
TaaI ACNGT 3 cut(s) 109, 642, 830
TaiI ACGT 5 cut(s) 415, 564, 713, 826, 1033
TaqI TCGA 2 cut(s) 774, 844
TasI AATT 9 cut(s) 165, 179, 337, 476, 674, 816, 951, 1038, 1096
TatI WGTACW 2 cut(s) 984, 996
TauI GCSGC 2 cut(s) 142, 216
TfiI GAWTC 1 cut(s) 203
Tru1I TTAA 2 cut(s) 53, 819
Tru9I TTAA 2 cut(s) 53, 819
TscAI CASTG 1 cut(s) 520
TseI GCWGC 5 cut(s) 57, 127, 130, 133, 136
TspDTI ATGAA 4 cut(s) 172, 216, 739, 1068
TspRI CASTG 1 cut(s) 520
VpaK11BI GGWCC 2 cut(s) 541, 784
XapI RAATTY 3 cut(s) 179, 476, 674
XcmI CCANNNNNNNNNTGG 1 cut(s) 349
XhoI CTCGAG 1 cut(s) 773
XspI CTAG 3 cut(s) 261, 660, 962
ZrmI AGTACT 2 cut(s) 986, 998
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.