Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0011822

Physical Location & Seq

Genomic Coordinates Forward (+)

2833346 .. 2835390

Gene Structure 2045 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G09230.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1182 bp

ATGGAGGCAGAGATCGTGAGACGATCGGAGGTAACGGGATTAAGAAGGGAGGTGGAAGAATCGTCAATTGGTAGAGGAGATTGCGATGGTGATGGCGGCGATGTGGGAGAAGATGCGGCAGGGTTCGTTGGGACGAGCGGGAGAGGAAGAAGAGATCGAGTTAAAGGGCCGTGGTCGAAGGAGGAGGATGATGTGTTGAGTGAGCTCGTTAAGAGGTTGGGAGCGAGGAATTGGAGTTTTATCGCTCGGAGTATTCCTGGTCGTTCAGGCAAGTCTTGTCGTCTTCGTTGGTGTAATCAGCTCAATCCAAATCTTATACGCAATTCATTTACTGAGGTAGAGGATCAGGCTATCATCGCAGCACATGCCATCCACGGAAACAAATGGGCTGTTATCGCGAAGCTCCTCCCCGGAAGAACAGATAATGCTATCAAGAACCACTGGAACTCTGCTTTAAGACGTCGATTCATAGACTTTGAAAAGGCCAAGAATATAGGAACTGGAAGCTTGGTCGTGGATGATTCTGGATTTGACAGAACGACAACAGTAGCCTCATCAGAAGAAACTTTATCTTCAGGCGGTGGTTGCCATGTAACTACTCCAATTGTATCTCCAGAAGGCAAAGAAGCTACCACCTCCATGGAAATGTCTGAAGAACAATGCGTAGAGAAAACAAACGGAGAAGGTATTTCTAGGCAAGATGATAAGGATCCTCCAACGCTTTTCCGCCCAGTGCCTCGGCTCAGTTCTTTTAATGCTTGCAATCACATGGAAGGATCACCCTCTCCACATATACAAGACCAAAATCAGCTCCAATCATCTAAACAAGACGCAGCAATGCTAAGATTGCTTGAAGGAGCTTACAGCGAACGGTTTGTGCCTCAAACATGTGGAGGTGGTTGTTGCAGCAACAATCCCGATGGCAGTTTTCAGCAAGAATCATTGTTGGGTCCAGAGTTTGTGGATTACTTAGACTCACCAACGTTTCCGAGTTCCGAACTAGCTGCTATAGCAACGGAAATAGGCAGCCTCGCTTGGCTGAGAAGCGGTTTAGAGAGTAGCAGCGTGAGGGTGATGGAAGACGCAGTTGGTCGGTTAAGGCCTCAAGGCTCCAGGGGTCATCGAGATCATTATCTTGTATCTGAACAGGGGACGAACATAACCAATGTCCTGTCCACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006950 GO:0006952 GO:0006970 GO:0007275 GO:0008150 GO:0009414 GO:0009415 GO:0009555 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009737 GO:0009739 GO:0009751 GO:0009753 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010646 GO:0010647 GO:0010928 GO:0010929 GO:0014070 GO:0019219 GO:0019222 GO:0023051 GO:0023056 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0033993 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0046677 GO:0046686 GO:0048229 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0097305 GO:0098542 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1903506 GO:2000022 GO:2000031 GO:2000112 GO:2001023 GO:2001038 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-binding

Protein Analysis

393

Amino Acids

42.81

Weight (kDa)

5.37

Isoelectric Point (pI)

64.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	55 - 101	3.1e-17	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	58 - 117	3.4e-15	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	107 - 151	1.3e-16	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	110 - 163	5.6e-07	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011822)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09230
fragaria_vesca	FvH4_6g12440
malus_domestica	MD04G1142200.v1.1 MD12G1156800.v1.1
prunus_persica	Prupe.6G267600_v2.0.a1
pyrus_communis	pycom04g12920 pycom12g14900
rosa_chinensis	RchiOBHm_Chr3g0464251
rosa_laevigata	RLG00000024713
rosa_multiflora	Rmu_co7985966.1_g000001 Rmu_sc0007811.1_g000007 Rmu_sc0024604.1_g000003
rosa_roxburghii	Rroxscaffold_6G00415900
rosa_rugosa	Rorug03G0071100 Rorug03G0071100
rosa_samantha	Rh3BG132900 Rh3CG135100 Rh3DG134300
rosa_wichuraiana	Rw3G010870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	463
AccBSI	CCGCTC	1 cut(s)	138
AccII	CGCG	1 cut(s)	398
AciI	CCGC	6 cut(s)	96, 116, 138, 579, 727, 1047
AclI	AACGTT	1 cut(s)	983
AclWI	GGATC	4 cut(s)	351, 704, 717, 784
AcuI	CTGAAG	2 cut(s)	558, 672
AcyI	GRCGYC	1 cut(s)	460
AflIII	ACRYGT	1 cut(s)	887
AgsI	TTSAA	2 cut(s)	479, 854
AjnI	CCWGG	2 cut(s)	256, 1112
AjuI	GAANNNNNNNTTGG	4 cut(s)	51, 83, 1071, 1103
AluBI	AGCT	8 cut(s)	205, 301, 403, 507, 629, 811, 860, 1004
AluI	AGCT	8 cut(s)	205, 301, 403, 507, 629, 811, 860, 1004
Alw21I	GWGCWC	1 cut(s)	207
Alw26I	GTCTC	1 cut(s)	13
AlwI	GGATC	4 cut(s)	351, 704, 717, 784
AoxI	GGCC	3 cut(s)	167, 483, 1100
ApeKI	GCWGC	6 cut(s)	359, 833, 906, 1004, 1026, 1062
AspS9I	GGNCC	2 cut(s)	167, 950
AsuC2I	CCSGG	1 cut(s)	411
AsuHPI	GGTGA	4 cut(s)	101, 771, 969, 1084
AvaII	GGWCC	1 cut(s)	950
BamHI	GGATCC	1 cut(s)	709
BanII	GRGCYC	1 cut(s)	207
BbsI	GAAGAC	2 cut(s)	275, 1086
Bbv12I	GWGCWC	1 cut(s)	207
BbvI	GCAGC	6 cut(s)	371, 845, 918, 991, 1038, 1074
BccI	CCATC	5 cut(s)	80, 86, 377, 914, 1069
BceAI	ACGGC	1 cut(s)	154
BcgI	CGANNNNNNTGC	2 cut(s)	986, 1020
BciT130I	CCWGG	2 cut(s)	258, 1114
BcnI	CCSGG	1 cut(s)	411
BcoDI	GTCTC	1 cut(s)	13
BfaI	CTAG	2 cut(s)	693, 1001
BfmI	CTRYAG	1 cut(s)	1008
BisI	GCNGC	8 cut(s)	97, 117, 360, 834, 907, 1005, 1027, 1063
BlsI	GCNGC	8 cut(s)	98, 118, 361, 835, 908, 1006, 1028, 1064
Bme1390I	CCNGG	3 cut(s)	258, 411, 1114
Bme18I	GGWCC	1 cut(s)	950
BmgT120I	GGNCC	2 cut(s)	167, 950
BmiI	GGNNCC	3 cut(s)	711, 951, 1111
BmrFI	CCNGG	3 cut(s)	258, 411, 1114
BmrI	ACTGGG	1 cut(s)	725
BmsI	GCATC	1 cut(s)	103
BmuI	ACTGGG	1 cut(s)	725
BpiI	GAAGAC	2 cut(s)	275, 1086
BpmI	CTGGAG	2 cut(s)	597, 1096
BpuEI	CTTGAG	1 cut(s)	1089
BpuMI	CCSGG	1 cut(s)	411
BsaBI	GATNNNNATC	2 cut(s)	708, 1131
BsaHI	GRCGYC	1 cut(s)	460
BsaJI	CCNNGG	6 cut(s)	170, 373, 409, 639, 737, 1113
Bse1I	ACTGG	3 cut(s)	446, 505, 731
Bse3DI	GCAATG	1 cut(s)	843
Bse8I	GATNNNNATC	2 cut(s)	708, 1131
BseBI	CCWGG	2 cut(s)	258, 1114
BseDI	CCNNGG	6 cut(s)	170, 373, 409, 639, 737, 1113
BseGI	GGATG	3 cut(s)	193, 369, 523
BseJI	GATNNNNATC	2 cut(s)	708, 1131
BseMI	GCAATG	1 cut(s)	843
BseMII	CTCAG	3 cut(s)	324, 757, 1031
BseNI	ACTGG	3 cut(s)	446, 505, 731
BseRI	GAGGAG	3 cut(s)	90, 197, 395
BseXI	GCAGC	6 cut(s)	371, 845, 918, 991, 1038, 1074
Bsh1236I	CGCG	1 cut(s)	398
Bsh1285I	CGRYCG	1 cut(s)	26
BshFI	GGCC	3 cut(s)	169, 485, 1102
BsiEI	CGRYCG	1 cut(s)	26
BsiHKAI	GWGCWC	1 cut(s)	207
BsiSI	CCGG	1 cut(s)	411
BslFI	GGGAC	2 cut(s)	145, 1165
BsmAI	GTCTC	1 cut(s)	13
BsmBI	CGTCTC	1 cut(s)	13
BsmFI	GGGAC	2 cut(s)	145, 1165
BsnI	GGCC	3 cut(s)	169, 485, 1102
Bsp1286I	GDGCHC	1 cut(s)	207
Bsp143I	GATC	7 cut(s)	12, 23, 154, 343, 709, 776, 1126
Bsp19I	CCATGG	1 cut(s)	639
Bsp68I	TCGCGA	1 cut(s)	398
BspACI	CCGC	6 cut(s)	96, 116, 138, 579, 727, 1047
BspANI	GGCC	3 cut(s)	169, 485, 1102
BspCNI	CTCAG	3 cut(s)	325, 756, 1032
BspFNI	CGCG	1 cut(s)	398
BspLI	GGNNCC	3 cut(s)	711, 951, 1111
BspPI	GGATC	4 cut(s)	351, 704, 717, 784
BsrBI	CCGCTC	1 cut(s)	138
BsrDI	GCAATG	1 cut(s)	843
BsrI	ACTGG	3 cut(s)	446, 505, 731
BssECI	CCNNGG	6 cut(s)	170, 373, 409, 639, 737, 1113
BssMI	GATC	7 cut(s)	12, 23, 154, 343, 709, 776, 1126
BssNI	GRCGYC	1 cut(s)	460
BssT1I	CCWWGG	1 cut(s)	639
Bst2UI	CCWGG	2 cut(s)	258, 1114
Bst4CI	ACNGT	2 cut(s)	547, 873
Bst6I	CTCTTC	1 cut(s)	145
BstACI	GRCGYC	1 cut(s)	460
BstAPI	GCANNNNNTGC	1 cut(s)	365
BstC8I	GCNNGC	1 cut(s)	760
BstDEI	CTNAG	5 cut(s)	333, 743, 842, 970, 1040
BstDSI	CCRYGG	3 cut(s)	170, 373, 639
BstF5I	GGATG	3 cut(s)	193, 369, 523
BstFNI	CGCG	1 cut(s)	398
BstKTI	GATC	7 cut(s)	15, 26, 157, 346, 712, 779, 1129
BstMAI	GTCTC	1 cut(s)	13
BstMBI	GATC	7 cut(s)	12, 23, 154, 343, 709, 776, 1126
BstMCI	CGRYCG	1 cut(s)	26
BstMWI	GCNNNNNNNGC	6 cut(s)	356, 365, 395, 585, 847, 1010
BstNI	CCWGG	2 cut(s)	258, 1114
BstNSI	RCATGY	2 cut(s)	368, 891
BstSCI	CCNGG	3 cut(s)	256, 409, 1112
BstSFI	CTRYAG	1 cut(s)	1008
BstUI	CGCG	1 cut(s)	398
BstV1I	GCAGC	6 cut(s)	371, 845, 918, 991, 1038, 1074
BstV2I	GAAGAC	2 cut(s)	275, 1086
BstX2I	RGATCY	1 cut(s)	709
BstXI	CCANNNNNNTGG	1 cut(s)	640
BstYI	RGATCY	1 cut(s)	709
BsuRI	GGCC	3 cut(s)	169, 485, 1102
BtgI	CCRYGG	3 cut(s)	170, 373, 639
BtgZI	GCGATG	3 cut(s)	99, 114, 340
BtsCI	GGATG	3 cut(s)	193, 369, 523
BtsIMutI	CAGTG	2 cut(s)	439, 738
BtuMI	TCGCGA	1 cut(s)	398
Cac8I	GCNNGC	1 cut(s)	760
Cfr13I	GGNCC	2 cut(s)	167, 950
CseI	GACGC	2 cut(s)	839, 1091
CviAII	CATG	5 cut(s)	365, 590, 640, 769, 888
DdeI	CTNAG	5 cut(s)	333, 743, 842, 970, 1040
DpnI	GATC	7 cut(s)	14, 25, 156, 345, 711, 778, 1128
DpnII	GATC	7 cut(s)	12, 23, 154, 343, 709, 776, 1126
Eam1104I	CTCTTC	1 cut(s)	145
EarI	CTCTTC	1 cut(s)	145
EciI	GGCGGA	1 cut(s)	716
Ecl136II	GAGCTC	1 cut(s)	205
Eco130I	CCWWGG	1 cut(s)	639
Eco147I	AGGCCT	1 cut(s)	1102
Eco24I	GRGCYC	1 cut(s)	207
Eco47I	GGWCC	1 cut(s)	950
Eco53kI	GAGCTC	1 cut(s)	205
Eco57I	CTGAAG	2 cut(s)	558, 672
EcoICRI	GAGCTC	1 cut(s)	205
EcoRII	CCWGG	2 cut(s)	256, 1112
EcoT14I	CCWWGG	1 cut(s)	639
EcoT38I	GRGCYC	1 cut(s)	207
ErhI	CCWWGG	1 cut(s)	639
Esp3I	CGTCTC	1 cut(s)	13
FaeI	CATG	5 cut(s)	368, 593, 643, 772, 891
FaqI	GGGAC	2 cut(s)	145, 1165
FatI	CATG	5 cut(s)	364, 589, 639, 768, 887
FauI	CCCGC	1 cut(s)	131
Fnu4HI	GCNGC	8 cut(s)	97, 117, 360, 834, 907, 1005, 1027, 1063
FokI	GGATG	3 cut(s)	200, 356, 530
FriOI	GRGCYC	1 cut(s)	207
Fsp4HI	GCNGC	8 cut(s)	97, 117, 360, 834, 907, 1005, 1027, 1063
FspBI	CTAG	2 cut(s)	693, 1001
GluI	GCNGC	8 cut(s)	97, 117, 360, 834, 907, 1005, 1027, 1063
GsuI	CTGGAG	2 cut(s)	597, 1096
HaeIII	GGCC	3 cut(s)	169, 485, 1102
HapII	CCGG	1 cut(s)	411
HgaI	GACGC	2 cut(s)	839, 1091
Hin1I	GRCGYC	1 cut(s)	460
Hin1II	CATG	5 cut(s)	368, 593, 643, 772, 891
HindIII	AAGCTT	1 cut(s)	505
HinfI	GANTC	5 cut(s)	59, 465, 521, 938, 974
HpaII	CCGG	1 cut(s)	411
HphI	GGTGA	4 cut(s)	101, 771, 969, 1084
Hpy166II	GTNNAC	1 cut(s)	1176
Hpy188I	TCNGA	7 cut(s)	28, 249, 559, 652, 990, 997, 1144
Hpy188III	TCNNGA	8 cut(s)	16, 397, 433, 525, 614, 917, 953, 1124
Hpy8I	GTNNAC	1 cut(s)	1176
Hpy99I	CGWCG	1 cut(s)	465
HpyAV	CCTTC	6 cut(s)	39, 172, 611, 677, 767, 848
HpyCH4III	ACNGT	2 cut(s)	547, 873
HpyCH4IV	ACGT	2 cut(s)	460, 983
HpyCH4V	TGCA	2 cut(s)	762, 906
HpyF10VI	GCNNNNNNNGC	6 cut(s)	356, 365, 395, 585, 847, 1010
HpyF3I	CTNAG	5 cut(s)	333, 743, 842, 970, 1040
HpySE526I	ACGT	2 cut(s)	460, 983
Hsp92I	GRCGYC	1 cut(s)	460
Hsp92II	CATG	5 cut(s)	368, 593, 643, 772, 891
Kzo9I	GATC	7 cut(s)	12, 23, 154, 343, 709, 776, 1126
LmnI	GCTCC	5 cut(s)	221, 408, 816, 857, 1115
Lsp1109I	GCAGC	6 cut(s)	371, 845, 918, 991, 1038, 1074
LweI	GCATC	1 cut(s)	103
MaeI	CTAG	2 cut(s)	693, 1001
MaeII	ACGT	2 cut(s)	460, 983
MaeIII	GTNAC	2 cut(s)	31, 592
MalI	GATC	7 cut(s)	14, 25, 156, 345, 711, 778, 1128
MbiI	CCGCTC	1 cut(s)	138
MboI	GATC	7 cut(s)	12, 23, 154, 343, 709, 776, 1126
MfeI	CAATTG	2 cut(s)	66, 603
MflI	RGATCY	1 cut(s)	709
MhlI	GDGCHC	1 cut(s)	207
MluCI	AATT	4 cut(s)	66, 229, 322, 603
MlyI	GAGTC	1 cut(s)	968
MmeI	TCCRAC	1 cut(s)	740
MseI	TTAA	6 cut(s)	41, 162, 210, 455, 753, 1097
MslI	CAYNNNNRTG	2 cut(s)	638, 644
MspI	CCGG	1 cut(s)	411
MspR9I	CCNGG	3 cut(s)	258, 411, 1114
MunI	CAATTG	2 cut(s)	66, 603
MvaI	CCWGG	2 cut(s)	258, 1114
MvnI	CGCG	1 cut(s)	398
MwoI	GCNNNNNNNGC	6 cut(s)	356, 365, 395, 585, 847, 1010
NciI	CCSGG	1 cut(s)	411
NcoI	CCATGG	1 cut(s)	639
NdeII	GATC	7 cut(s)	12, 23, 154, 343, 709, 776, 1126
NlaIII	CATG	5 cut(s)	368, 593, 643, 772, 891
NlaIV	GGNNCC	3 cut(s)	711, 951, 1111
NmeAIII	GCCGAG	1 cut(s)	718
NruI	TCGCGA	1 cut(s)	398
NspI	RCATGY	2 cut(s)	368, 891
PceI	AGGCCT	1 cut(s)	1102
PciI	ACATGT	1 cut(s)	887
PfeI	GAWTC	4 cut(s)	59, 465, 521, 938
PkrI	GCNGC	8 cut(s)	98, 118, 361, 835, 908, 1006, 1028, 1064
Ple19I	CGATCG	1 cut(s)	26
PleI	GAGTC	1 cut(s)	968
PpsI	GAGTC	1 cut(s)	968
PscI	ACATGT	1 cut(s)	887
Psp124BI	GAGCTC	1 cut(s)	207
Psp1406I	AACGTT	1 cut(s)	983
Psp6I	CCWGG	2 cut(s)	256, 1112
PspGI	CCWGG	2 cut(s)	256, 1112
PspN4I	GGNNCC	3 cut(s)	711, 951, 1111
PspPI	GGNCC	2 cut(s)	167, 950
PsuI	RGATCY	1 cut(s)	709
PvuI	CGATCG	1 cut(s)	26
RruI	TCGCGA	1 cut(s)	398
RseI	CAYNNNNRTG	2 cut(s)	638, 644
SacI	GAGCTC	1 cut(s)	207
SaqAI	TTAA	6 cut(s)	41, 162, 210, 455, 753, 1097
SatI	GCNGC	8 cut(s)	97, 117, 360, 834, 907, 1005, 1027, 1063
Sau3AI	GATC	7 cut(s)	12, 23, 154, 343, 709, 776, 1126
Sau96I	GGNCC	2 cut(s)	167, 950
SchI	GAGTC	1 cut(s)	968
ScrFI	CCNGG	3 cut(s)	258, 411, 1114
SduI	GDGCHC	1 cut(s)	207
SfaNI	GCATC	1 cut(s)	103
SfcI	CTRYAG	1 cut(s)	1008
SinI	GGWCC	1 cut(s)	950
SmiMI	CAYNNNNRTG	2 cut(s)	638, 644
SmlI	CTYRAG	1 cut(s)	1104
SmoI	CTYRAG	1 cut(s)	1104
Sse9I	AATT	4 cut(s)	66, 229, 322, 603
SseBI	AGGCCT	1 cut(s)	1102
SsiI	CCGC	6 cut(s)	96, 116, 138, 579, 727, 1047
SspMI	CTAG	2 cut(s)	693, 1001
SstI	GAGCTC	1 cut(s)	207
StuI	AGGCCT	1 cut(s)	1102
StyD4I	CCNGG	3 cut(s)	256, 409, 1112
StyI	CCWWGG	1 cut(s)	639
TaaI	ACNGT	2 cut(s)	547, 873
TaiI	ACGT	2 cut(s)	463, 986
TaqI	TCGA	4 cut(s)	157, 176, 463, 1123
TasI	AATT	4 cut(s)	66, 229, 322, 603
TauI	GCSGC	2 cut(s)	99, 119
TfiI	GAWTC	4 cut(s)	59, 465, 521, 938
Tru1I	TTAA	6 cut(s)	41, 162, 210, 455, 753, 1097
Tru9I	TTAA	6 cut(s)	41, 162, 210, 455, 753, 1097
TscAI	CASTG	2 cut(s)	446, 738
TseI	GCWGC	6 cut(s)	359, 833, 906, 1004, 1026, 1062
TspDTI	ATGAA	2 cut(s)	315, 457
TspGWI	ACGGA	3 cut(s)	390, 693, 1031
TspRI	CASTG	2 cut(s)	446, 738
VpaK11BI	GGWCC	1 cut(s)	950
XceI	RCATGY	2 cut(s)	368, 891
XspI	CTAG	2 cut(s)	693, 1001
ZraI	GACGTC	1 cut(s)	461

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.