AT3G11860

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006591
Physical Location & Seq
Forward (+)
3744816 .. 3746850
2035 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G11860.1

Sequence Viewer

Length: 759 bp
ATGGGAACTGTGGATATTTTCAATGGCTACAAGATGTATAAGTGGGGTGAGGCGAGATCTAAACGCGGTGAGGAGATAGCGACGATGGATGTTATAATTAGACAGTTGGAACTGAGCAAGGCTGGTCCTTCCAAGTTAGGATTTGATGGAGAAGAAGACGCTTGGGTCTTTGTTAAAAAGCAGAGACTCTTCATTGTCTCGCCTTCTCTTCCACTTCCTCAACAACAGCATTTCACTTTGGAGAAACCTGCAATATCTCCTCAATTAGAAGCTGGGTTTAGAGAAAGCATGGAGGTTACGCAAGATAGTACCTTTGTTCACACTGTGGTGCCTTCTCTTCCTCTGCCTGAACATTTCATTAGGCATAAGCCTGAAACTTCTGAGTCTCAAGCTGAATTAAGAGACGTTCTTGCGGATCCGCACAAAACTACTCATAAGCCTGCGTCTTCTCAGTCACAAGTTGAGTTCAGAGACGTCATGGAGGATACGCATGGAACTACTCCTCTTCAAACAGAGATGTCTTCTCTTCCATTACCTCAACACTTCGTTCTGCAGAAGCCTGCTGGAACTTCTCAGTCAAAAGCGGATACACGCAAAGCTACTCTTGTTCACACGGTGATGCCTTCTCTTCCTGTACCTGAACATTACATTCTGCAATTTGTTTTTAATAATATAAGTGAAAAGAATATGATATATGAATTCCTTATATCTTTTTTGTTTCAAACTTTCAAGTATTATCTGCAATACGATAAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

252

Amino Acids

28.83

Weight (kDa)

5.94

Isoelectric Point (pI)

63.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 95
AasI GACNNNNNNGTC 1 cut(s) 164
AatII GACGTC 1 cut(s) 477
Acc36I ACCTGC 1 cut(s) 256
AccB1I GGYRCC 1 cut(s) 328
AccII CGCG 1 cut(s) 66
AciI CCGC 4 cut(s) 66, 413, 419, 584
AclWI GGATC 2 cut(s) 410, 423
AcsI RAATTY 1 cut(s) 698
AcyI GRCGYC 1 cut(s) 474
AdeI CACNNNGTG 2 cut(s) 325, 616
AfaI GTAC 2 cut(s) 310, 636
AgsI TTSAA 4 cut(s) 22, 509, 722, 730
AleI CACNNNNGTG 1 cut(s) 326
AluBI AGCT 3 cut(s) 272, 392, 599
AluI AGCT 3 cut(s) 272, 392, 599
Alw26I GTCTC 5 cut(s) 178, 202, 390, 396, 465
AlwI GGATC 2 cut(s) 410, 423
ApoI RAATTY 1 cut(s) 698
AspS9I GGNCC 1 cut(s) 125
AsuHPI GGTGA 3 cut(s) 59, 80, 628
AvaII GGWCC 1 cut(s) 125
BamHI GGATCC 1 cut(s) 415
BanI GGYRCC 1 cut(s) 328
BbsI GAAGAC 3 cut(s) 162, 438, 513
BccI CCATC 2 cut(s) 79, 140
BciVI GTATCC 2 cut(s) 478, 580
BcoDI GTCTC 5 cut(s) 178, 202, 390, 396, 465
BfmI CTRYAG 1 cut(s) 551
BfuAI ACCTGC 1 cut(s) 256
BfuI GTATCC 2 cut(s) 478, 580
BglII AGATCT 1 cut(s) 56
Bme18I GGWCC 1 cut(s) 125
BmgT120I GGNCC 1 cut(s) 125
BmiI GGNNCC 2 cut(s) 330, 417
BmsI GCATC 1 cut(s) 609
BpiI GAAGAC 3 cut(s) 162, 438, 513
BpuEI CTTGAG 1 cut(s) 372
BsaHI GRCGYC 1 cut(s) 474
BseGI GGATG 1 cut(s) 94
BseMII CTCAG 4 cut(s) 104, 372, 464, 587
BseRI GAGGAG 3 cut(s) 86, 249, 492
BseYI CCCAGC 1 cut(s) 272
Bsh1236I CGCG 1 cut(s) 66
BshNI GGYRCC 1 cut(s) 328
BsmAI GTCTC 5 cut(s) 178, 202, 390, 396, 465
BsmBI CGTCTC 2 cut(s) 396, 465
Bsp143I GATC 2 cut(s) 56, 415
BspACI CCGC 4 cut(s) 66, 413, 419, 584
BspCNI CTCAG 4 cut(s) 105, 373, 463, 586
BspFNI CGCG 1 cut(s) 66
BspLI GGNNCC 2 cut(s) 330, 417
BspMAI CTGCAG 1 cut(s) 555
BspMI ACCTGC 1 cut(s) 256
BspPI GGATC 2 cut(s) 410, 423
BspT107I GGYRCC 1 cut(s) 328
BssMI GATC 2 cut(s) 56, 415
BssNI GRCGYC 1 cut(s) 474
Bst4CI ACNGT 4 cut(s) 10, 105, 325, 616
Bst6I CTCTTC 6 cut(s) 194, 213, 342, 510, 531, 633
BstACI GRCGYC 1 cut(s) 474
BstC8I GCNNGC 2 cut(s) 441, 561
BstDEI CTNAG 4 cut(s) 113, 381, 450, 573
BstF5I GGATG 1 cut(s) 94
BstFNI CGCG 1 cut(s) 66
BstKTI GATC 2 cut(s) 59, 418
BstMAI GTCTC 5 cut(s) 178, 202, 390, 396, 465
BstMBI GATC 2 cut(s) 56, 415
BstSFI CTRYAG 1 cut(s) 551
BstUI CGCG 1 cut(s) 66
BstV2I GAAGAC 3 cut(s) 162, 438, 513
BstX2I RGATCY 2 cut(s) 56, 415
BstYI RGATCY 2 cut(s) 56, 415
BsuI GTATCC 2 cut(s) 478, 580
BtsCI GGATG 1 cut(s) 94
BtsIMutI CAGTG 1 cut(s) 321
BveI ACCTGC 1 cut(s) 256
Cac8I GCNNGC 2 cut(s) 441, 561
Cfr13I GGNCC 1 cut(s) 125
CseI GACGC 2 cut(s) 167, 432
Csp6I GTAC 2 cut(s) 309, 635
CviAII CATG 3 cut(s) 289, 478, 491
CviJI RGCY 8 cut(s) 27, 122, 272, 370, 392, 439, 559, 599
CviKI_1 RGCY 8 cut(s) 27, 122, 272, 370, 392, 439, 559, 599
CviQI GTAC 2 cut(s) 309, 635
DdeI CTNAG 4 cut(s) 113, 381, 450, 573
DpnI GATC 2 cut(s) 58, 417
DpnII GATC 2 cut(s) 56, 415
DraIII CACNNNGTG 2 cut(s) 325, 616
DrdI GACNNNNNNGTC 1 cut(s) 164
DseDI GACNNNNNNGTC 1 cut(s) 164
Eam1104I CTCTTC 6 cut(s) 194, 213, 342, 510, 531, 633
EarI CTCTTC 6 cut(s) 194, 213, 342, 510, 531, 633
Eco47I GGWCC 1 cut(s) 125
EcoRI GAATTC 1 cut(s) 698
Esp3I CGTCTC 2 cut(s) 396, 465
FaeI CATG 3 cut(s) 292, 481, 494
FalI AAGNNNNNCTT 2 cut(s) 588, 620
FatI CATG 3 cut(s) 288, 477, 490
FokI GGATG 1 cut(s) 101
GsaI CCCAGC 1 cut(s) 276
HgaI GACGC 2 cut(s) 167, 432
Hin1I GRCGYC 1 cut(s) 474
Hin1II CATG 3 cut(s) 292, 481, 494
HinfI GANTC 2 cut(s) 186, 383
HphI GGTGA 3 cut(s) 59, 80, 628
Hpy166II GTNNAC 2 cut(s) 319, 610
Hpy188I TCNGA 2 cut(s) 382, 470
Hpy8I GTNNAC 2 cut(s) 319, 610
Hpy99I CGWCG 1 cut(s) 85
HpyAV CCTTC 4 cut(s) 138, 213, 342, 633
HpyCH4III ACNGT 4 cut(s) 10, 105, 325, 616
HpyCH4IV ACGT 2 cut(s) 405, 474
HpyCH4V TGCA 4 cut(s) 251, 553, 655, 742
HpyF3I CTNAG 4 cut(s) 113, 381, 450, 573
HpySE526I ACGT 2 cut(s) 405, 474
Hsp92I GRCGYC 1 cut(s) 474
Hsp92II CATG 3 cut(s) 292, 481, 494
Kzo9I GATC 2 cut(s) 56, 415
LweI GCATC 1 cut(s) 609
MaeII ACGT 2 cut(s) 405, 474
MaeIII GTNAC 2 cut(s) 295, 453
MalI GATC 2 cut(s) 58, 417
MboI GATC 2 cut(s) 56, 415
MflI RGATCY 2 cut(s) 56, 415
MluCI AATT 5 cut(s) 96, 263, 395, 656, 698
MlyI GAGTC 2 cut(s) 180, 392
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 9 cut(s) 43, 64, 228, 270, 286, 351, 475, 513, 546
MseI TTAA 3 cut(s) 174, 398, 666
MslI CAYNNNNRTG 2 cut(s) 326, 617
MvnI CGCG 1 cut(s) 66
NdeII GATC 2 cut(s) 56, 415
NlaIII CATG 3 cut(s) 292, 481, 494
NlaIV GGNNCC 2 cut(s) 330, 417
NmuCI GTSAC 1 cut(s) 453
OliI CACNNNNGTG 1 cut(s) 326
PleI GAGTC 2 cut(s) 180, 391
PpsI GAGTC 2 cut(s) 180, 391
PsiI TTATAA 1 cut(s) 95
PspFI CCCAGC 1 cut(s) 272
PspN4I GGNNCC 2 cut(s) 330, 417
PspPI GGNCC 1 cut(s) 125
PstI CTGCAG 1 cut(s) 555
PsuI RGATCY 2 cut(s) 56, 415
RsaI GTAC 2 cut(s) 310, 636
RsaNI GTAC 2 cut(s) 309, 635
RseI CAYNNNNRTG 2 cut(s) 326, 617
SaqAI TTAA 3 cut(s) 174, 398, 666
Sau3AI GATC 2 cut(s) 56, 415
Sau96I GGNCC 1 cut(s) 125
SchI GAGTC 2 cut(s) 180, 392
SfaNI GCATC 1 cut(s) 609
SfcI CTRYAG 1 cut(s) 551
SinI GGWCC 1 cut(s) 125
SmiMI CAYNNNNRTG 2 cut(s) 326, 617
SmlI CTYRAG 1 cut(s) 387
SmoI CTYRAG 1 cut(s) 387
Sse9I AATT 5 cut(s) 96, 263, 395, 656, 698
SsiI CCGC 4 cut(s) 66, 413, 419, 584
TaaI ACNGT 4 cut(s) 10, 105, 325, 616
TaiI ACGT 2 cut(s) 408, 477
TasI AATT 5 cut(s) 96, 263, 395, 656, 698
Tru1I TTAA 3 cut(s) 174, 398, 666
Tru9I TTAA 3 cut(s) 174, 398, 666
TscAI CASTG 1 cut(s) 328
TseFI GTSAC 1 cut(s) 453
Tsp45I GTSAC 1 cut(s) 453
TspDTI ATGAA 3 cut(s) 181, 346, 711
TspRI CASTG 1 cut(s) 328
VpaK11BI GGWCC 1 cut(s) 125
XapI RAATTY 1 cut(s) 698
ZraI GACGTC 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.