AT3G11890

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006591
Physical Location & Seq
Forward (+)
3755456 .. 3757842
2387 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G11890.1

Sequence Viewer

Length: 1503 bp
ATGGCGAAGCTAAGGCCTAGACAGTTGGAACTGAGCAAGACTGTTCCTTCCAAGTTGGGTTTTGATGGAGAAGAAGACGCTTGGGTCTTTGTTAAAAAGCAGAAAATCTTCATTGTCTTGCCTTCTCTTCCACTTCCTCAACAACAGCATTTCACTTTGGAGAAACCTGCAATATCTCCTCAATTAGAAGCTGGGTTTAGAGAAAGCATGGAGGTTACGCAAGATAGTACCTTTGTTCACACTGTGGTGCCTTCTCTTCCTCTGCCTGAAACTCCTCAATCACAAGCTGAGTTAAGAGACGCTCTTGCGGATACACACTTTACCACTCCTGTTCCCACTGTGGTGGTACCTGCTCTTCCTCTACCTGAACAATTCATTTTGCACAAGCCTGAAACTTCTCATTCGCAAGTTCAGTTCAGAGATTGTATTGCGAATACGCATAAAACTACTCCTCTTCACACAGTGGTGCATTCTCTTCCGGTAACTGAACATTCCACTTTGCAGAAGCCTACATCTTCCCAGTCACAAGTTGAGTTAAGAGAGTCTATTGCGGATACTCATGGAACTACTCCTCTTCAAACAGAGATTTCTTCTCTTCCATTACCTCAACACTTCGTTTTGCAGAAGCCCGCAACTTCTCAATCACAAGCGGATACACACGAAACTACTCTTCTTCACACAATACTGCCTTCTCTTCCTGTACCTGAACTATGTACTTTGCAGAAGCCTGCAACTTCTCAATCACAAGCTGAGTTAAGAGCGAATACGCGCAAAGCTACTCTTGTTCACGCAGCAGCGATGCCTTCTCTTCCTGTACCTGAACATTACAGTCTACAGAAGCCTTCAACTTCTCAATCACAAGCTGAGTTAAGAGCGAAGACGCGCAAAGCTACTCTTGTTCACGCAGCAGCGATACCTTCTCTTCCTGTACCTGAACATTACAGTCTACTCAAGCCTTCAACTTCTCAATCACAAGCTGAGAAGCCTGCAACTTCTCAATCGCAAGCTGAAACTAGTCGTGTTCAAACTGTGGAACCTGAGGCCTGTCCTGATTTCACCTCAGTAGACAAACCGGAGATAGTTATGAGTCGCAGTCTCACAACCAGGAAAGCTCCAGCACCAAAGAGATCACTGCAAGAAAGTAGGAAGAATCAGGATAGAAGAGTGGAGATCCACAGGAGGCGTGCGGGTCACAAGCCCATAAGGTTTCCAAGAGTGATGTGTAGCTCTGTTGTTATGGATAATGAGAAGCTGAGAGTGTTGAATCTGGAGAAGAAGGTTGAGAAAGCGGGAGGGTTGAACGAATGGGTTGGATCTATAGGATTAGGGAGAGAGTTTGAGAGGATGTTGAGAGGACAGAGGATGAGTAAGTTTCAGATGGCGAATTTGACAATGGAGAAGCTTAAGCAGATGGGTGCTTTAGCCGTTGGGCCAAGAAGAAAGCTTATACATGCAATAGGTTGTGTCTATCACCCACATTGTCTACGTGCTTCCTTTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

500

Amino Acids

55.62

Weight (kDa)

9.51

Isoelectric Point (pI)

67.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_2 PF07647 431 - 489 1.2e-12 SAM domain (Sterile alpha motif)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 83
Acc36I ACCTGC 2 cut(s) 175, 358
Acc65I GGTACC 1 cut(s) 346
AccB1I GGYRCC 2 cut(s) 247, 346
AccI GTMKAC 4 cut(s) 832, 946, 1065, 1483
AccII CGCG 2 cut(s) 769, 883
AciI CCGC 6 cut(s) 308, 551, 630, 650, 1187, 1289
AclWI GGATC 2 cut(s) 1165, 1321
AcsI RAATTY 1 cut(s) 1384
AdeI CACNNNGTG 2 cut(s) 244, 463
AfaI GTAC 6 cut(s) 229, 348, 702, 715, 816, 930
AflII CTTAAG 1 cut(s) 1403
AgsI TTSAA 6 cut(s) 578, 846, 960, 1025, 1264, 1300
AhlI ACTAGT 1 cut(s) 1013
AjnI CCWGG 1 cut(s) 1103
AleI CACNNNNGTG 3 cut(s) 245, 341, 464
Alw26I GTCTC 2 cut(s) 291, 1100
AlwI GGATC 2 cut(s) 1165, 1321
AoxI GGCC 3 cut(s) 14, 1041, 1430
ApeKI GCWGC 4 cut(s) 791, 794, 905, 908
ApoI RAATTY 1 cut(s) 1384
Asp700I GAANNNNTTC 1 cut(s) 107
Asp718I GGTACC 1 cut(s) 346
AspLEI GCGC 2 cut(s) 771, 885
AspS9I GGNCC 1 cut(s) 1430
AsuHPI GGTGA 2 cut(s) 1048, 1463
AxyI CCTNAGG 1 cut(s) 1038
BanI GGYRCC 2 cut(s) 247, 346
BarI GAAGNNNNNNTAC 2 cut(s) 339, 371
BbsI GAAGAC 2 cut(s) 81, 884
BbvI GCAGC 4 cut(s) 803, 806, 917, 920
BccI CCATC 3 cut(s) 59, 1372, 1405
BceAI ACGGC 1 cut(s) 1409
BciT130I CCWGG 1 cut(s) 1105
BciVI GTATCC 3 cut(s) 304, 547, 646
BcoDI GTCTC 2 cut(s) 291, 1100
BcuI ACTAGT 1 cut(s) 1013
BfaI CTAG 2 cut(s) 18, 1014
BfmI CTRYAG 2 cut(s) 833, 1317
BfrI CTTAAG 1 cut(s) 1403
BfuAI ACCTGC 2 cut(s) 175, 358
BfuI GTATCC 3 cut(s) 304, 547, 646
BisI GCNGC 4 cut(s) 792, 795, 906, 909
BlsI GCNGC 4 cut(s) 793, 796, 907, 910
Bme1390I CCNGG 1 cut(s) 1105
BmgT120I GGNCC 1 cut(s) 1430
BmiI GGNNCC 3 cut(s) 249, 348, 1035
BmrFI CCNGG 1 cut(s) 1105
BmrI ACTGGG 1 cut(s) 514
BmsI GCATC 1 cut(s) 789
BmuI ACTGGG 1 cut(s) 514
BpiI GAAGAC 2 cut(s) 81, 884
BpmI CTGGAG 2 cut(s) 1098, 1289
Bpu10I CCTNAGC 1 cut(s) 11
BpuEI CTTGAG 1 cut(s) 935
BsaAI YACGTR 1 cut(s) 1487
BsaWI WCCGGW 2 cut(s) 478, 1072
Bse1I ACTGG 1 cut(s) 520
Bse21I CCTNAGG 1 cut(s) 1038
BseBI CCWGG 1 cut(s) 1105
BseGI GGATG 2 cut(s) 1350, 1368
BseMII CTCAG 8 cut(s) 23, 279, 741, 855, 969, 1029, 1074, 1244
BseNI ACTGG 1 cut(s) 520
BseRI GAGGAG 4 cut(s) 168, 264, 441, 561
BseXI GCAGC 4 cut(s) 803, 806, 917, 920
BseYI CCCAGC 1 cut(s) 191
Bsh1236I CGCG 2 cut(s) 769, 883
BshFI GGCC 3 cut(s) 16, 1043, 1432
BshNI GGYRCC 2 cut(s) 247, 346
BsiSI CCGG 2 cut(s) 479, 1073
BsmAI GTCTC 2 cut(s) 291, 1100
BsmBI CGTCTC 1 cut(s) 291
BsmI GAATGC 1 cut(s) 469
BsnI GGCC 3 cut(s) 16, 1043, 1432
Bsp143I GATC 3 cut(s) 1127, 1170, 1313
BspACI CCGC 6 cut(s) 308, 551, 630, 650, 1187, 1289
BspANI GGCC 3 cut(s) 16, 1043, 1432
BspCNI CTCAG 8 cut(s) 24, 280, 742, 856, 970, 1030, 1073, 1245
BspFNI CGCG 2 cut(s) 769, 883
BspLI GGNNCC 3 cut(s) 249, 348, 1035
BspMI ACCTGC 2 cut(s) 175, 358
BspPI GGATC 2 cut(s) 1165, 1321
BspQI GCTCTTC 1 cut(s) 360
BspT107I GGYRCC 2 cut(s) 247, 346
BspTI CTTAAG 1 cut(s) 1403
BsrI ACTGG 1 cut(s) 520
BssMI GATC 3 cut(s) 1127, 1170, 1313
Bst2UI CCWGG 1 cut(s) 1105
Bst4CI ACNGT 8 cut(s) 24, 43, 244, 340, 463, 830, 944, 1030
BstAFI CTTAAG 1 cut(s) 1403
BstBAI YACGTR 1 cut(s) 1487
BstC8I GCNNGC 5 cut(s) 630, 729, 987, 1005, 1185
BstDEI CTNAG 9 cut(s) 11, 32, 288, 750, 864, 978, 1038, 1060, 1253
BstF5I GGATG 2 cut(s) 1350, 1368
BstFNI CGCG 2 cut(s) 769, 883
BstHHI GCGC 2 cut(s) 771, 885
BstKTI GATC 3 cut(s) 1130, 1173, 1316
BstMAI GTCTC 2 cut(s) 291, 1100
BstMBI GATC 3 cut(s) 1127, 1170, 1313
BstNI CCWGG 1 cut(s) 1105
BstNSI RCATGY 1 cut(s) 1454
BstSCI CCNGG 1 cut(s) 1103
BstSFI CTRYAG 2 cut(s) 833, 1317
BstUI CGCG 2 cut(s) 769, 883
BstV1I GCAGC 4 cut(s) 803, 806, 917, 920
BstV2I GAAGAC 2 cut(s) 81, 884
BstX2I RGATCY 2 cut(s) 1170, 1313
BstXI CCANNNNNNTGG 1 cut(s) 343
BstYI RGATCY 2 cut(s) 1170, 1313
Bsu36I CCTNAGG 1 cut(s) 1038
BsuI GTATCC 3 cut(s) 304, 547, 646
BsuRI GGCC 3 cut(s) 16, 1043, 1432
BtgZI GCGATG 1 cut(s) 812
BtsCI GGATG 2 cut(s) 1350, 1368
BtsI GCAGTG 1 cut(s) 1130
BtsIMutI CAGTG 4 cut(s) 240, 336, 468, 1130
BveI ACCTGC 2 cut(s) 175, 358
Cac8I GCNNGC 5 cut(s) 630, 729, 987, 1005, 1185
CfoI GCGC 2 cut(s) 771, 885
Cfr13I GGNCC 1 cut(s) 1430
CseI GACGC 3 cut(s) 86, 308, 889
Csp6I GTAC 6 cut(s) 228, 347, 701, 714, 815, 929
CviAII CATG 3 cut(s) 208, 560, 1451
CviQI GTAC 6 cut(s) 228, 347, 701, 714, 815, 929
DdeI CTNAG 9 cut(s) 11, 32, 288, 750, 864, 978, 1038, 1060, 1253
DpnI GATC 3 cut(s) 1129, 1172, 1315
DpnII GATC 3 cut(s) 1127, 1170, 1313
DraIII CACNNNGTG 2 cut(s) 244, 463
DrdI GACNNNNNNGTC 1 cut(s) 83
DseDI GACNNNNNNGTC 1 cut(s) 83
Eco147I AGGCCT 2 cut(s) 16, 1043
Eco81I CCTNAGG 1 cut(s) 1038
EcoRII CCWGG 1 cut(s) 1103
Esp3I CGTCTC 1 cut(s) 291
FaeI CATG 3 cut(s) 211, 563, 1454
FalI AAGNNNNNCTT 4 cut(s) 765, 797, 879, 911
FatI CATG 3 cut(s) 207, 559, 1450
FauI CCCGC 3 cut(s) 637, 1180, 1282
FblI GTMKAC 4 cut(s) 832, 946, 1065, 1483
Fnu4HI GCNGC 4 cut(s) 792, 795, 906, 909
FokI GGATG 2 cut(s) 1357, 1375
Fsp4HI GCNGC 4 cut(s) 792, 795, 906, 909
FspBI CTAG 2 cut(s) 18, 1014
GlaI GCGC 2 cut(s) 770, 884
GluI GCNGC 4 cut(s) 792, 795, 906, 909
GsaI CCCAGC 1 cut(s) 195
GsuI CTGGAG 2 cut(s) 1098, 1289
HaeIII GGCC 3 cut(s) 16, 1043, 1432
HapII CCGG 2 cut(s) 479, 1073
HgaI GACGC 3 cut(s) 86, 308, 889
HhaI GCGC 2 cut(s) 771, 885
Hin1II CATG 3 cut(s) 211, 563, 1454
Hin6I GCGC 2 cut(s) 769, 883
HinP1I GCGC 2 cut(s) 769, 883
HindIII AAGCTT 2 cut(s) 1400, 1442
HinfI GANTC 4 cut(s) 542, 1087, 1150, 1264
HpaII CCGG 2 cut(s) 479, 1073
HphI GGTGA 2 cut(s) 1048, 1463
Hpy166II GTNNAC 7 cut(s) 238, 787, 833, 901, 947, 1066, 1484
Hpy188I TCNGA 2 cut(s) 419, 1377
Hpy188III TCNNGA 3 cut(s) 1049, 1154, 1268
Hpy8I GTNNAC 7 cut(s) 238, 787, 833, 901, 947, 1066, 1484
HpyAV CCTTC 9 cut(s) 57, 132, 261, 699, 813, 852, 927, 966, 1270
HpyCH4III ACNGT 8 cut(s) 24, 43, 244, 340, 463, 830, 944, 1030
HpyCH4IV ACGT 1 cut(s) 1486
HpyF3I CTNAG 9 cut(s) 11, 32, 288, 750, 864, 978, 1038, 1060, 1253
HpySE526I ACGT 1 cut(s) 1486
Hsp92II CATG 3 cut(s) 211, 563, 1454
HspAI GCGC 2 cut(s) 769, 883
KpnI GGTACC 1 cut(s) 350
Kzo9I GATC 3 cut(s) 1127, 1170, 1313
LguI GCTCTTC 1 cut(s) 360
LmnI GCTCC 1 cut(s) 1117
Lsp1109I GCAGC 4 cut(s) 803, 806, 917, 920
LweI GCATC 1 cut(s) 789
MaeI CTAG 2 cut(s) 18, 1014
MaeII ACGT 1 cut(s) 1486
MaeIII GTNAC 4 cut(s) 214, 481, 522, 1190
MalI GATC 3 cut(s) 1129, 1172, 1315
MboI GATC 3 cut(s) 1127, 1170, 1313
MflI RGATCY 2 cut(s) 1170, 1313
MluCI AATT 3 cut(s) 182, 371, 1384
MlyI GAGTC 2 cut(s) 551, 1096
MmeI TCCRAC 2 cut(s) 6, 1291
MroXI GAANNNNTTC 1 cut(s) 107
MseI TTAA 7 cut(s) 93, 293, 536, 755, 869, 1404, 1497
MslI CAYNNNNRTG 3 cut(s) 245, 341, 464
MspCI CTTAAG 1 cut(s) 1403
MspI CCGG 2 cut(s) 479, 1073
MspR9I CCNGG 1 cut(s) 1105
Mva1269I GAATGC 1 cut(s) 469
MvaI CCWGG 1 cut(s) 1105
MvnI CGCG 2 cut(s) 769, 883
NdeII GATC 3 cut(s) 1127, 1170, 1313
NlaIII CATG 3 cut(s) 211, 563, 1454
NlaIV GGNNCC 3 cut(s) 249, 348, 1035
NmuCI GTSAC 2 cut(s) 522, 1190
NspI RCATGY 1 cut(s) 1454
OliI CACNNNNGTG 3 cut(s) 245, 341, 464
PceI AGGCCT 2 cut(s) 16, 1043
PciSI GCTCTTC 1 cut(s) 360
PctI GAATGC 1 cut(s) 469
PdmI GAANNNNTTC 1 cut(s) 107
PfeI GAWTC 2 cut(s) 1150, 1264
PkrI GCNGC 4 cut(s) 793, 796, 907, 910
PleI GAGTC 2 cut(s) 550, 1095
PpsI GAGTC 2 cut(s) 550, 1095
Ppu21I YACGTR 1 cut(s) 1487
Psp6I CCWGG 1 cut(s) 1103
PspFI CCCAGC 1 cut(s) 191
PspGI CCWGG 1 cut(s) 1103
PspN4I GGNNCC 3 cut(s) 249, 348, 1035
PspPI GGNCC 1 cut(s) 1430
PsuI RGATCY 2 cut(s) 1170, 1313
RsaI GTAC 6 cut(s) 229, 348, 702, 715, 816, 930
RsaNI GTAC 6 cut(s) 228, 347, 701, 714, 815, 929
RseI CAYNNNNRTG 3 cut(s) 245, 341, 464
SapI GCTCTTC 1 cut(s) 360
SaqAI TTAA 7 cut(s) 93, 293, 536, 755, 869, 1404, 1497
SatI GCNGC 4 cut(s) 792, 795, 906, 909
Sau3AI GATC 3 cut(s) 1127, 1170, 1313
Sau96I GGNCC 1 cut(s) 1430
SchI GAGTC 2 cut(s) 551, 1096
ScrFI CCNGG 1 cut(s) 1105
SfaNI GCATC 1 cut(s) 789
SfcI CTRYAG 2 cut(s) 833, 1317
SmiMI CAYNNNNRTG 3 cut(s) 245, 341, 464
SmlI CTYRAG 2 cut(s) 950, 1403
SmoI CTYRAG 2 cut(s) 950, 1403
SpeI ACTAGT 1 cut(s) 1013
Sse9I AATT 3 cut(s) 182, 371, 1384
SseBI AGGCCT 2 cut(s) 16, 1043
SsiI CCGC 6 cut(s) 308, 551, 630, 650, 1187, 1289
SspMI CTAG 2 cut(s) 18, 1014
StuI AGGCCT 2 cut(s) 16, 1043
StyD4I CCNGG 1 cut(s) 1103
TaaI ACNGT 8 cut(s) 24, 43, 244, 340, 463, 830, 944, 1030
TaiI ACGT 1 cut(s) 1489
TasI AATT 3 cut(s) 182, 371, 1384
TatI WGTACW 1 cut(s) 713
TfiI GAWTC 2 cut(s) 1150, 1264
Tru1I TTAA 7 cut(s) 93, 293, 536, 755, 869, 1404, 1497
Tru9I TTAA 7 cut(s) 93, 293, 536, 755, 869, 1404, 1497
TscAI CASTG 4 cut(s) 247, 343, 468, 1137
TseFI GTSAC 2 cut(s) 522, 1190
TseI GCWGC 4 cut(s) 791, 794, 905, 908
Tsp45I GTSAC 2 cut(s) 522, 1190
TspDTI ATGAA 2 cut(s) 100, 364
TspRI CASTG 4 cut(s) 247, 343, 468, 1137
Vha464I CTTAAG 1 cut(s) 1403
XapI RAATTY 1 cut(s) 1384
XceI RCATGY 1 cut(s) 1454
XmiI GTMKAC 4 cut(s) 832, 946, 1065, 1483
XmnI GAANNNNTTC 1 cut(s) 107
XspI CTAG 2 cut(s) 18, 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.