Rroxscaffold_6G00403840

Sterile alpha motif.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0006591
Physical Location & Seq
Reverse (-)
26300637 .. 26305699
5063 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00403840.1

Sequence Viewer

Length: 840 bp
ATGGATATAGGGGGAATTCTAAACCCAGTTCCCTATTCAAACACAGATTGTCTGCTAATGGCGAAGAAAAGGCAAAGACAGCTGGTGGAAAATCCCCCGCACAAGAAAAGTACTCCGAGTGGTGGTCTTATTGACGCTTCAAACTCAGACAACGATCTGGATCTGCAGGATAGTTGGGTGATAGTCAAAAAGCAGAGAGTCAACATTTTAGTACCTGGACTTCCTGTTGCTAATAAATCACCACTCCCAAGCCCAGAACCAAGTCAGCTGCAACTCGTGGCTAGAGAAACAATAGAAAGTGGACCACAGCTTCCTGCTGATACACATCCCAAAACGGCTTTAGTTCATGAGAAAAAGAAGATTAAACCTGTTGCCCCTAAAAGGGCTGTGCAACTTGCTAAGAAAGCTTCTCCTGCTGCTGAATACGTTCCAACCGTTTCCCAGTCAGTGAGGCGAGAATTAAGCACAAAATCACGAACCCCAGATCAGATGGCTACTTCACAGTGTCAGAGAGCTCTAGGAGTATCTATGACCTCGAAAGCCATCATGCAGCGAAGAAGATTACAACATGTTTTCCTGGATCAGGGAATGGTGCTGAATGAAAGGCTGAGAGCTCGGAATATTGAGAGGAAGCTTCAGAATGCTGGTGGGTTAAGCAGGTGGTTAGCATCATTGGGACTGGAGCAGTTTGTTAGAATTTTTCAGAGGAAAGGTTTCAGTAAATTTCAGTTGGTGAATTTGAACATGAAAAAGCTCAAAGATATGGGTGCAAATGCAGTTGGGCCAAGAAGGAAATTGATGCATGCAATAGACTGCTTTTGCCAACCATCCTATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAM_2

Protein Analysis

279

Amino Acids

31.16

Weight (kDa)

10.25

Isoelectric Point (pI)

65.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_2 PF07647 217 - 272 1.4e-14 SAM domain (Sterile alpha motif)
SAM_1 PF00536 218 - 272 3.4e-09 SAM domain (Sterile alpha motif)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 648
Acc36I ACCTGC 1 cut(s) 648
AciI CCGC 1 cut(s) 98
AclWI GGATC 2 cut(s) 168, 588
AcsI RAATTY 4 cut(s) 15, 696, 722, 736
AcuI CTGAAG 1 cut(s) 620
AfaI GTAC 2 cut(s) 112, 213
AfiI CCNNNNNNNGG 4 cut(s) 122, 381, 382, 583
AflIII ACRYGT 1 cut(s) 568
AgsI TTSAA 3 cut(s) 39, 141, 742
AjnI CCWGG 2 cut(s) 214, 576
AluBI AGCT 8 cut(s) 82, 268, 310, 407, 515, 614, 634, 754
AluI AGCT 8 cut(s) 82, 268, 310, 407, 515, 614, 634, 754
Alw21I GWGCWC 2 cut(s) 517, 616
AlwI GGATC 2 cut(s) 168, 588
AoxI GGCC 1 cut(s) 782
ApeKI GCWGC 3 cut(s) 268, 416, 550
ApoI RAATTY 4 cut(s) 15, 696, 722, 736
Asp700I GAANNNNTTC 2 cut(s) 426, 713
AspS9I GGNCC 2 cut(s) 302, 782
AsuHPI GGTGA 3 cut(s) 190, 231, 745
AvaII GGWCC 1 cut(s) 302
BanII GRGCYC 2 cut(s) 517, 616
BarI GAAGNNNNNNTAC 2 cut(s) 204, 236
BauI CACGAG 1 cut(s) 275
Bbv12I GWGCWC 2 cut(s) 517, 616
BbvI GCAGC 3 cut(s) 255, 403, 562
BccI CCATC 3 cut(s) 484, 551, 835
BceAI ACGGC 1 cut(s) 351
BciT130I CCWGG 2 cut(s) 216, 578
BfaI CTAG 2 cut(s) 282, 518
BfmI CTRYAG 1 cut(s) 164
BfuAI ACCTGC 1 cut(s) 648
BisI GCNGC 3 cut(s) 269, 417, 551
BlsI GCNGC 3 cut(s) 270, 418, 552
BmcAI AGTACT 1 cut(s) 112
Bme1390I CCNGG 2 cut(s) 216, 578
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 2 cut(s) 302, 782
BmrFI CCNGG 2 cut(s) 216, 578
BmrI ACTGGG 2 cut(s) 20, 436
BmsI GCATC 2 cut(s) 677, 789
BmuI ACTGGG 2 cut(s) 20, 436
BpmI CTGGAG 1 cut(s) 701
BsaBI GATNNNNATC 2 cut(s) 159, 324
Bsc4I CCNNNNNNNGG 4 cut(s) 122, 381, 382, 583
Bse1I ACTGG 3 cut(s) 26, 442, 684
Bse8I GATNNNNATC 2 cut(s) 159, 324
BseBI CCWGG 2 cut(s) 216, 578
BseGI GGATG 2 cut(s) 325, 827
BseJI GATNNNNATC 2 cut(s) 159, 324
BseLI CCNNNNNNNGG 4 cut(s) 122, 381, 382, 583
BseMII CTCAG 2 cut(s) 159, 599
BseNI ACTGG 3 cut(s) 26, 442, 684
BseXI GCAGC 3 cut(s) 255, 403, 562
BshFI GGCC 1 cut(s) 784
BsiHKAI GWGCWC 2 cut(s) 517, 616
BslFI GGGAC 1 cut(s) 690
BslI CCNNNNNNNGG 4 cut(s) 122, 381, 382, 583
BsmFI GGGAC 1 cut(s) 690
BsmI GAATGC 1 cut(s) 646
BsnI GGCC 1 cut(s) 784
Bsp1286I GDGCHC 2 cut(s) 517, 616
Bsp143I GATC 4 cut(s) 154, 160, 484, 580
BspACI CCGC 1 cut(s) 98
BspANI GGCC 1 cut(s) 784
BspCNI CTCAG 2 cut(s) 158, 600
BspHI TCATGA 1 cut(s) 346
BspMAI CTGCAG 1 cut(s) 168
BspMI ACCTGC 1 cut(s) 648
BspPI GGATC 2 cut(s) 168, 588
BsrI ACTGG 3 cut(s) 26, 442, 684
BssMI GATC 4 cut(s) 154, 160, 484, 580
BssSI CACGAG 1 cut(s) 275
Bst2BI CACGAG 1 cut(s) 275
Bst2UI CCWGG 2 cut(s) 216, 578
Bst4CI ACNGT 2 cut(s) 436, 504
BstC8I GCNNGC 1 cut(s) 804
BstDEI CTNAG 3 cut(s) 145, 399, 608
BstENI CCTNNNNNAGG 1 cut(s) 581
BstF5I GGATG 2 cut(s) 325, 827
BstKTI GATC 4 cut(s) 157, 163, 487, 583
BstMBI GATC 4 cut(s) 154, 160, 484, 580
BstMWI GCNNNNNNNGC 3 cut(s) 79, 404, 413
BstNI CCWGG 2 cut(s) 216, 578
BstNSI RCATGY 2 cut(s) 572, 806
BstSCI CCNGG 2 cut(s) 214, 576
BstSFI CTRYAG 1 cut(s) 164
BstV1I GCAGC 3 cut(s) 255, 403, 562
BstX2I RGATCY 1 cut(s) 160
BstYI RGATCY 1 cut(s) 160
BsuRI GGCC 1 cut(s) 784
BtsCI GGATG 2 cut(s) 325, 827
BtsIMutI CAGTG 2 cut(s) 453, 509
BveI ACCTGC 1 cut(s) 648
Cac8I GCNNGC 1 cut(s) 804
CciI TCATGA 1 cut(s) 346
Cfr13I GGNCC 2 cut(s) 302, 782
CseI GACGC 1 cut(s) 143
Csp6I GTAC 2 cut(s) 111, 212
CviAII CATG 5 cut(s) 347, 547, 569, 745, 803
CviQI GTAC 2 cut(s) 111, 212
DdeI CTNAG 3 cut(s) 145, 399, 608
DpnI GATC 4 cut(s) 156, 162, 486, 582
DpnII GATC 4 cut(s) 154, 160, 484, 580
Ecl136II GAGCTC 2 cut(s) 515, 614
Eco24I GRGCYC 2 cut(s) 517, 616
Eco47I GGWCC 1 cut(s) 302
Eco53kI GAGCTC 2 cut(s) 515, 614
Eco57I CTGAAG 1 cut(s) 620
EcoICRI GAGCTC 2 cut(s) 515, 614
EcoNI CCTNNNNNAGG 1 cut(s) 581
EcoRI GAATTC 1 cut(s) 15
EcoRII CCWGG 2 cut(s) 214, 576
EcoT22I ATGCAT 1 cut(s) 804
EcoT38I GRGCYC 2 cut(s) 517, 616
FaeI CATG 5 cut(s) 350, 550, 572, 748, 806
FaiI YATR 8 cut(s) 8, 348, 530, 548, 570, 746, 764, 804
FaqI GGGAC 1 cut(s) 690
FatI CATG 5 cut(s) 346, 546, 568, 744, 802
FauI CCCGC 1 cut(s) 105
Fnu4HI GCNGC 3 cut(s) 269, 417, 551
FokI GGATG 2 cut(s) 312, 814
FriOI GRGCYC 2 cut(s) 517, 616
Fsp4HI GCNGC 3 cut(s) 269, 417, 551
FspBI CTAG 2 cut(s) 282, 518
GluI GCNGC 3 cut(s) 269, 417, 551
GsuI CTGGAG 1 cut(s) 701
HaeIII GGCC 1 cut(s) 784
HgaI GACGC 1 cut(s) 143
Hin1II CATG 5 cut(s) 350, 550, 572, 748, 806
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HindIII AAGCTT 2 cut(s) 405, 632
HinfI GANTC 1 cut(s) 198
HphI GGTGA 3 cut(s) 190, 231, 745
Hpy166II GTNNAC 2 cut(s) 202, 302
Hpy188I TCNGA 7 cut(s) 117, 148, 489, 510, 618, 639, 705
Hpy188III TCNNGA 3 cut(s) 158, 347, 474
Hpy8I GTNNAC 2 cut(s) 202, 302
HpyAV CCTTC 1 cut(s) 783
HpyCH4III ACNGT 2 cut(s) 436, 504
HpyCH4IV ACGT 1 cut(s) 426
HpyCH4V TGCA 8 cut(s) 166, 271, 391, 550, 770, 776, 802, 806
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 404, 413
HpyF3I CTNAG 3 cut(s) 145, 399, 608
HpySE526I ACGT 1 cut(s) 426
Hsp92II CATG 5 cut(s) 350, 550, 572, 748, 806
Kzo9I GATC 4 cut(s) 154, 160, 484, 580
LmnI GCTCC 1 cut(s) 682
Lsp1109I GCAGC 3 cut(s) 255, 403, 562
LweI GCATC 2 cut(s) 677, 789
MaeI CTAG 2 cut(s) 282, 518
MaeII ACGT 1 cut(s) 426
MalI GATC 4 cut(s) 156, 162, 486, 582
MboI GATC 4 cut(s) 154, 160, 484, 580
MboII GAAGA 4 cut(s) 76, 370, 567, 570
MflI RGATCY 1 cut(s) 160
MhlI GDGCHC 2 cut(s) 517, 616
MluCI AATT 6 cut(s) 15, 458, 696, 722, 736, 794
MlyI GAGTC 1 cut(s) 207
MmeI TCCRAC 1 cut(s) 455
MnlI CCTC 4 cut(s) 444, 544, 621, 699
Mph1103I ATGCAT 1 cut(s) 804
MroXI GAANNNNTTC 2 cut(s) 426, 713
MseI TTAA 4 cut(s) 363, 461, 653, 838
MspA1I CMGCKG 2 cut(s) 82, 268
MspR9I CCNGG 2 cut(s) 216, 578
Mva1269I GAATGC 1 cut(s) 646
MvaI CCWGG 2 cut(s) 216, 578
MwoI GCNNNNNNNGC 3 cut(s) 79, 404, 413
NdeII GATC 4 cut(s) 154, 160, 484, 580
NlaIII CATG 5 cut(s) 350, 550, 572, 748, 806
NsiI ATGCAT 1 cut(s) 804
NspI RCATGY 2 cut(s) 572, 806
PaeI GCATGC 1 cut(s) 806
PagI TCATGA 1 cut(s) 346
PaqCI CACCTGC 1 cut(s) 648
PciI ACATGT 1 cut(s) 568
PcsI WCGNNNNNNNCGW 1 cut(s) 432
PctI GAATGC 1 cut(s) 646
PdmI GAANNNNTTC 2 cut(s) 426, 713
PfoI TCCNGGA 1 cut(s) 576
PkrI GCNGC 3 cut(s) 270, 418, 552
PleI GAGTC 1 cut(s) 206
PpsI GAGTC 1 cut(s) 206
PscI ACATGT 1 cut(s) 568
Psp124BI GAGCTC 2 cut(s) 517, 616
Psp6I CCWGG 2 cut(s) 214, 576
PspGI CCWGG 2 cut(s) 214, 576
PspPI GGNCC 2 cut(s) 302, 782
PstI CTGCAG 1 cut(s) 168
PsuI RGATCY 1 cut(s) 160
PvuII CAGCTG 2 cut(s) 82, 268
RsaI GTAC 2 cut(s) 112, 213
RsaNI GTAC 2 cut(s) 111, 212
SacI GAGCTC 2 cut(s) 517, 616
SaqAI TTAA 4 cut(s) 363, 461, 653, 838
SatI GCNGC 3 cut(s) 269, 417, 551
Sau3AI GATC 4 cut(s) 154, 160, 484, 580
Sau96I GGNCC 2 cut(s) 302, 782
ScaI AGTACT 1 cut(s) 112
SchI GAGTC 1 cut(s) 207
ScrFI CCNGG 2 cut(s) 216, 578
SduI GDGCHC 2 cut(s) 517, 616
SfaNI GCATC 2 cut(s) 677, 789
SfcI CTRYAG 1 cut(s) 164
SinI GGWCC 1 cut(s) 302
SphI GCATGC 1 cut(s) 806
Sse9I AATT 6 cut(s) 15, 458, 696, 722, 736, 794
SsiI CCGC 1 cut(s) 98
SspI AATATT 1 cut(s) 622
SspMI CTAG 2 cut(s) 282, 518
SstI GAGCTC 2 cut(s) 517, 616
StyD4I CCNGG 2 cut(s) 214, 576
TaaI ACNGT 2 cut(s) 436, 504
TaiI ACGT 1 cut(s) 429
TaqI TCGA 1 cut(s) 536
TasI AATT 6 cut(s) 15, 458, 696, 722, 736, 794
TatI WGTACW 1 cut(s) 110
Tru1I TTAA 4 cut(s) 363, 461, 653, 838
Tru9I TTAA 4 cut(s) 363, 461, 653, 838
TscAI CASTG 2 cut(s) 453, 509
TseI GCWGC 3 cut(s) 268, 416, 550
TspDTI ATGAA 3 cut(s) 335, 615, 761
TspRI CASTG 2 cut(s) 453, 509
VpaK11BI GGWCC 1 cut(s) 302
XagI CCTNNNNNAGG 1 cut(s) 581
XapI RAATTY 4 cut(s) 15, 696, 722, 736
XceI RCATGY 2 cut(s) 572, 806
XmnI GAANNNNTTC 2 cut(s) 426, 713
XspI CTAG 2 cut(s) 282, 518
ZrmI AGTACT 1 cut(s) 112
Zsp2I ATGCAT 1 cut(s) 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.