Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006330

Physical Location & Seq

Genomic Coordinates Forward (+)

3919463 .. 3921519

Gene Structure 2057 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G12290.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 900 bp

ATGGCGTCATCATCTGATCACACGGCGAAGATAATCGACGGCAAGGCGATTGCTCATACCATCAGATCAGAGATCGCCGAGGAAGTTCGCGGTCTATCTGAGAAACACGGCAAGGTCCCAGGACTAGCTGTAGTTATTGTTGGGAGCCGAAAGGATTCACAGACCTATGTGAATACGAAGAGGAAAGCGTGCGCTGAGGTTGGGATTAAGTCATTTGACGTGGGCCTACCAGAGGAAGTTTCTGAAGCTGATCTTATTAGCAAAGTTCATGAACTAAATTCAAATCCGGATGTCCATGGCATATTAGTTCAACTCCCATTGCCGAAACATATTAATGAGGAGCATATATTGGGTGCAATCAGCATTGATAAAGATGTCGATGGCTTCCATCCTTTGAATATTGGTAAGCTAGCCATGAAAGGCAGAGAACCCCTCTTCCTTCCTTGCACCCCAAAGGGATGTTTGGAACTCCTAGCTAGAAGCGGCGTAAAGATAAAGGGGCAACGAGCAGTTGTTGTAGGTCGGAGTAACATTGTTGGTTTGCCCGTTTCACTTCTTTTGCTCAAGGCTGATGCTACTGTCACAACTGTACATTCTCACACCAAGGATCCTGAGGCTATCATACGGGAAGCTGACATTGTTATTGCTGCATGCGGACAAGCCCACATGATTAAGGGCAACTGGATAAAGCCAGGGGCTGCAGTAATTGATGTTGGAACTAATGCAGTCAGCGACCCGAGCAAGAAATCAGGATACCGGTTGGTTGGAGATGTTGATTTCGCAGAAGCTTCAAAAGTTGCAGGTTTCATAACTCCGGTCCCTGGTGGTGTAGGCCCAATGACAGTGGCAATGCTTCTCAGGAACACCGTAGACGGTGCCAAGCGTGTCTTTGGCGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004477 GO:0004488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006575 GO:0006725 GO:0006730 GO:0006732 GO:0006760 GO:0006807 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0016491 GO:0016645 GO:0016646 GO:0016787 GO:0016810 GO:0016814 GO:0019219 GO:0019222 GO:0019238 GO:0019752 GO:0031323 GO:0034641 GO:0042558 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0044030 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046653 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051186 GO:0055114 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

THF_DHG_CYH THF_DHG_CYH_C

Protein Analysis

299

Amino Acids

31.59

Weight (kDa)

8.27

Isoelectric Point (pI)

28.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
THF_DHG_CYH	PF00763	12 - 127	1.5e-42	Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain
THF_DHG_CYH_C	PF02882	148 - 295	5.8e-61	Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006330)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12290
fragaria_vesca	FvH4_2g25270 FvH4_2g25270 FvH4_2g25270 FvH4_2g25270
malus_domestica	MD11G1304100.v1.1
prunus_persica	Prupe.1G144700_v2.0.a1 Prupe.8G257500_v2.0.a1
pyrus_communis	pycom03g22520 pycom11g26580
rosa_chinensis	RchiOBHm_Chr3g0495451 RchiOBHm_Chr6g0293401
rosa_laevigata	RLG00000011949
rosa_multiflora	Rmu_sc0005599.1_g000023 Rmu_sc0006269.1_g000022
rosa_roxburghii	Rroxscaffold_7G00174180
rosa_rugosa	Rorug03G0273500 Rorug03G0273600 Rorug06G0235300 Rorug06G0235400 Rorug06G0235400
rosa_samantha	Rh3BG356400 Rh3CG353300 Rh3DG355500 Rh6CG362100
rosa_wichuraiana	Rw3G028050 Rw6G030290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	791
AccB1I	GGYRCC	1 cut(s)	875
AccI	GTMKAC	1 cut(s)	870
AccII	CGCG	1 cut(s)	90
AccIII	TCCGGA	1 cut(s)	286
AciI	CCGC	3 cut(s)	90, 483, 654
AclWI	GGATC	2 cut(s)	602, 615
AcsI	RAATTY	1 cut(s)	277
AcuI	CTGAAG	1 cut(s)	264
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	1 cut(s)	591
AfiI	CCNNNNNNNGG	2 cut(s)	232, 821
AgeI	ACCGGT	1 cut(s)	756
AgsI	TTSAA	4 cut(s)	282, 311, 397, 792
AjiI	CACGTC	1 cut(s)	220
AjnI	CCWGG	3 cut(s)	118, 691, 820
AloI	GAACNNNNNNTCC	2 cut(s)	420, 452
AluBI	AGCT	6 cut(s)	128, 248, 409, 476, 632, 788
AluI	AGCT	6 cut(s)	128, 248, 409, 476, 632, 788
AlwI	GGATC	2 cut(s)	602, 615
AlwNI	CAGNNNCTG	1 cut(s)	698
Ama87I	CYCGRG	1 cut(s)	736
Aor13HI	TCCGGA	1 cut(s)	286
AoxI	GGCC	2 cut(s)	223, 832
ApeKI	GCWGC	2 cut(s)	647, 698
ApoI	RAATTY	1 cut(s)	277
AseI	ATTAAT	1 cut(s)	333
AsiGI	ACCGGT	1 cut(s)	756
Asp700I	GAANNNNTTC	1 cut(s)	154
AspLEI	GCGC	1 cut(s)	194
AspS9I	GGNCC	4 cut(s)	115, 223, 817, 833
AsuNHI	GCTAGC	1 cut(s)	409
AvaI	CYCGRG	1 cut(s)	736
AvaII	GGWCC	2 cut(s)	115, 817
AxyI	CCTNAGG	1 cut(s)	612
BamHI	GGATCC	1 cut(s)	607
BanI	GGYRCC	1 cut(s)	875
BbvCI	CCTCAGC	1 cut(s)	195
BbvI	GCAGC	2 cut(s)	634, 685
BccI	CCATC	3 cut(s)	68, 374, 396
BceAI	ACGGC	3 cut(s)	39, 55, 124
BciT130I	CCWGG	3 cut(s)	120, 693, 822
BciVI	GTATCC	1 cut(s)	746
BclI	TGATCA	1 cut(s)	16
BfaI	CTAG	4 cut(s)	125, 410, 473, 477
BfmI	CTRYAG	2 cut(s)	129, 699
BfuAI	ACCTGC	1 cut(s)	791
BfuI	GTATCC	1 cut(s)	746
BisI	GCNGC	3 cut(s)	484, 648, 699
BlsI	GCNGC	3 cut(s)	485, 649, 700
Bme1390I	CCNGG	3 cut(s)	120, 693, 822
Bme18I	GGWCC	2 cut(s)	115, 817
BmeT110I	CYCGRG	1 cut(s)	736
BmgBI	CACGTC	1 cut(s)	220
BmgT120I	GGNCC	4 cut(s)	115, 223, 817, 833
BmiI	GGNNCC	5 cut(s)	117, 146, 609, 819, 877
BmrFI	CCNGG	3 cut(s)	120, 693, 822
BmsI	GCATC	1 cut(s)	562
BmtI	GCTAGC	1 cut(s)	413
BplI	GAGNNNNNCTC	2 cut(s)	417, 449
Bpu10I	CCTNAGC	1 cut(s)	195
BpuEI	CTTGAG	1 cut(s)	548
BsaHI	GRCGYC	1 cut(s)	5
BsaJI	CCNNGG	6 cut(s)	78, 118, 295, 603, 692, 820
BsaWI	WCCGGW	3 cut(s)	286, 756, 814
Bsc4I	CCNNNNNNNGG	2 cut(s)	232, 821
Bse118I	RCCGGY	1 cut(s)	756
Bse1I	ACTGG	1 cut(s)	686
Bse21I	CCTNAGG	1 cut(s)	612
Bse3DI	GCAATG	2 cut(s)	317, 855
BseAI	TCCGGA	1 cut(s)	286
BseBI	CCWGG	3 cut(s)	120, 693, 822
BseDI	CCNNGG	6 cut(s)	78, 118, 295, 603, 692, 820
BseGI	GGATG	3 cut(s)	295, 388, 464
BseLI	CCNNNNNNNGG	2 cut(s)	232, 821
BseMI	GCAATG	2 cut(s)	317, 855
BseMII	CTCAG	4 cut(s)	90, 186, 603, 871
BseNI	ACTGG	1 cut(s)	686
BseRI	GAGGAG	1 cut(s)	353
BseXI	GCAGC	2 cut(s)	634, 685
Bsh1236I	CGCG	1 cut(s)	90
BshFI	GGCC	2 cut(s)	225, 834
BshNI	GGYRCC	1 cut(s)	875
BshTI	ACCGGT	1 cut(s)	756
BsiHKCI	CYCGRG	1 cut(s)	736
BsiSI	CCGG	3 cut(s)	287, 757, 815
BslFI	GGGAC	2 cut(s)	101, 803
BslI	CCNNNNNNNGG	2 cut(s)	232, 821
BsmFI	GGGAC	2 cut(s)	101, 803
BsnI	GGCC	2 cut(s)	225, 834
BsoBI	CYCGRG	1 cut(s)	736
Bsp13I	TCCGGA	1 cut(s)	286
Bsp1407I	TGTACA	1 cut(s)	589
Bsp143I	GATC	5 cut(s)	16, 65, 72, 250, 607
Bsp19I	CCATGG	1 cut(s)	295
BspACI	CCGC	3 cut(s)	90, 483, 654
BspANI	GGCC	2 cut(s)	225, 834
BspCNI	CTCAG	4 cut(s)	91, 187, 604, 870
BspEI	TCCGGA	1 cut(s)	286
BspFNI	CGCG	1 cut(s)	90
BspHI	TCATGA	1 cut(s)	268
BspLI	GGNNCC	5 cut(s)	117, 146, 609, 819, 877
BspMAI	CTGCAG	1 cut(s)	703
BspMI	ACCTGC	1 cut(s)	791
BspOI	GCTAGC	1 cut(s)	413
BspPI	GGATC	2 cut(s)	602, 615
BspT107I	GGYRCC	1 cut(s)	875
BsrDI	GCAATG	2 cut(s)	317, 855
BsrFI	RCCGGY	1 cut(s)	756
BsrGI	TGTACA	1 cut(s)	589
BsrI	ACTGG	1 cut(s)	686
BssAI	RCCGGY	1 cut(s)	756
BssECI	CCNNGG	6 cut(s)	78, 118, 295, 603, 692, 820
BssMI	GATC	5 cut(s)	16, 65, 72, 250, 607
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	2 cut(s)	295, 603
Bst2UI	CCWGG	3 cut(s)	120, 693, 822
Bst4CI	ACNGT	5 cut(s)	580, 589, 844, 868, 875
Bst6I	CTCTTC	2 cut(s)	173, 440
BstACI	GRCGYC	1 cut(s)	5
BstAUI	TGTACA	1 cut(s)	589
BstC8I	GCNNGC	3 cut(s)	190, 411, 652
BstDEI	CTNAG	4 cut(s)	99, 195, 612, 857
BstDSI	CCRYGG	1 cut(s)	295
BstENI	CCTNNNNNAGG	1 cut(s)	230
BstF5I	GGATG	3 cut(s)	295, 388, 464
BstFNI	CGCG	1 cut(s)	90
BstHHI	GCGC	1 cut(s)	194
BstKTI	GATC	5 cut(s)	19, 68, 75, 253, 610
BstMBI	GATC	5 cut(s)	16, 65, 72, 250, 607
BstMWI	GCNNNNNNNGC	1 cut(s)	738
BstNI	CCWGG	3 cut(s)	120, 693, 822
BstNSI	RCATGY	1 cut(s)	654
BstSCI	CCNGG	3 cut(s)	118, 691, 820
BstSFI	CTRYAG	2 cut(s)	129, 699
BstUI	CGCG	1 cut(s)	90
BstV1I	GCAGC	2 cut(s)	634, 685
BstX2I	RGATCY	1 cut(s)	607
BstYI	RGATCY	1 cut(s)	607
Bsu36I	CCTNAGG	1 cut(s)	612
BsuI	GTATCC	1 cut(s)	746
BsuRI	GGCC	2 cut(s)	225, 834
BtgI	CCRYGG	1 cut(s)	295
BtrI	CACGTC	1 cut(s)	220
BtsCI	GGATG	3 cut(s)	295, 388, 464
BtsIMutI	CAGTG	1 cut(s)	849
BveI	ACCTGC	1 cut(s)	791
Cac8I	GCNNGC	3 cut(s)	190, 411, 652
CaiI	CAGNNNCTG	1 cut(s)	698
CciI	TCATGA	1 cut(s)	268
CfoI	GCGC	1 cut(s)	194
Cfr10I	RCCGGY	1 cut(s)	756
Cfr13I	GGNCC	4 cut(s)	115, 223, 817, 833
Csp6I	GTAC	1 cut(s)	590
CspAI	ACCGGT	1 cut(s)	756
CspCI	CAANNNNNGTGG	2 cut(s)	825, 860
CviAII	CATG	5 cut(s)	269, 296, 415, 651, 667
CviQI	GTAC	1 cut(s)	590
DdeI	CTNAG	4 cut(s)	99, 195, 612, 857
DpnI	GATC	5 cut(s)	18, 67, 74, 252, 609
DpnII	GATC	5 cut(s)	16, 65, 72, 250, 607
Eam1104I	CTCTTC	2 cut(s)	173, 440
EarI	CTCTTC	2 cut(s)	173, 440
Eco130I	CCWWGG	2 cut(s)	295, 603
Eco47I	GGWCC	2 cut(s)	115, 817
Eco57I	CTGAAG	1 cut(s)	264
Eco81I	CCTNAGG	1 cut(s)	612
Eco88I	CYCGRG	1 cut(s)	736
EcoNI	CCTNNNNNAGG	1 cut(s)	230
EcoO109I	RGGNCCY	1 cut(s)	115
EcoRII	CCWGG	3 cut(s)	118, 691, 820
EcoT14I	CCWWGG	2 cut(s)	295, 603
ErhI	CCWWGG	2 cut(s)	295, 603
FaeI	CATG	5 cut(s)	272, 299, 418, 654, 670
FalI	AAGNNNNNCTT	3 cut(s)	237, 269, 872
FaqI	GGGAC	2 cut(s)	101, 803
FatI	CATG	5 cut(s)	268, 295, 414, 650, 666
FbaI	TGATCA	1 cut(s)	16
FblI	GTMKAC	1 cut(s)	870
Fnu4HI	GCNGC	3 cut(s)	484, 648, 699
FokI	GGATG	3 cut(s)	302, 375, 471
Fsp4HI	GCNGC	3 cut(s)	484, 648, 699
FspBI	CTAG	4 cut(s)	125, 410, 473, 477
GlaI	GCGC	1 cut(s)	193
GluI	GCNGC	3 cut(s)	484, 648, 699
HaeIII	GGCC	2 cut(s)	225, 834
HapII	CCGG	3 cut(s)	287, 757, 815
HhaI	GCGC	1 cut(s)	194
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	5 cut(s)	272, 299, 418, 654, 670
Hin6I	GCGC	1 cut(s)	192
HinP1I	GCGC	1 cut(s)	192
HindIII	AAGCTT	1 cut(s)	786
HinfI	GANTC	1 cut(s)	155
HpaII	CCGG	3 cut(s)	287, 757, 815
Hpy166II	GTNNAC	1 cut(s)	871
Hpy188I	TCNGA	6 cut(s)	16, 65, 70, 100, 244, 525
Hpy188III	TCNNGA	5 cut(s)	269, 287, 611, 750, 859
Hpy8I	GTNNAC	1 cut(s)	871
Hpy99I	CGWCG	1 cut(s)	41
HpyAV	CCTTC	1 cut(s)	449
HpyCH4III	ACNGT	5 cut(s)	580, 589, 844, 868, 875
HpyCH4IV	ACGT	1 cut(s)	219
HpyCH4V	TGCA	6 cut(s)	356, 447, 650, 701, 725, 800
HpyF10VI	GCNNNNNNNGC	1 cut(s)	738
HpyF3I	CTNAG	4 cut(s)	99, 195, 612, 857
HpySE526I	ACGT	1 cut(s)	219
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	5 cut(s)	272, 299, 418, 654, 670
HspAI	GCGC	1 cut(s)	192
Kpn2I	TCCGGA	1 cut(s)	286
Ksp22I	TGATCA	1 cut(s)	16
Kzo9I	GATC	5 cut(s)	16, 65, 72, 250, 607
LmnI	GCTCC	2 cut(s)	144, 340
Lsp1109I	GCAGC	2 cut(s)	634, 685
LweI	GCATC	1 cut(s)	562
MaeI	CTAG	4 cut(s)	125, 410, 473, 477
MaeII	ACGT	1 cut(s)	219
MaeIII	GTNAC	2 cut(s)	527, 580
MalI	GATC	5 cut(s)	18, 67, 74, 252, 609
MboI	GATC	5 cut(s)	16, 65, 72, 250, 607
MboII	GAAGA	3 cut(s)	40, 190, 427
MflI	RGATCY	1 cut(s)	607
MluCI	AATT	2 cut(s)	277, 705
MmeI	TCCRAC	3 cut(s)	503, 694, 745
MnlI	CCTC	7 cut(s)	73, 174, 190, 226, 331, 443, 607
MroI	TCCGGA	1 cut(s)	286
MroXI	GAANNNNTTC	1 cut(s)	154
MseI	TTAA	3 cut(s)	207, 333, 672
MslI	CAYNNNNRTG	1 cut(s)	333
MspI	CCGG	3 cut(s)	287, 757, 815
MspR9I	CCNGG	3 cut(s)	120, 693, 822
MvaI	CCWGG	3 cut(s)	120, 693, 822
MvnI	CGCG	1 cut(s)	90
MwoI	GCNNNNNNNGC	1 cut(s)	738
NcoI	CCATGG	1 cut(s)	295
NdeII	GATC	5 cut(s)	16, 65, 72, 250, 607
NheI	GCTAGC	1 cut(s)	409
NlaIII	CATG	5 cut(s)	272, 299, 418, 654, 670
NlaIV	GGNNCC	5 cut(s)	117, 146, 609, 819, 877
NmeAIII	GCCGAG	1 cut(s)	103
NmuCI	GTSAC	1 cut(s)	580
NspI	RCATGY	1 cut(s)	654
PaeI	GCATGC	1 cut(s)	654
PagI	TCATGA	1 cut(s)	268
PdmI	GAANNNNTTC	1 cut(s)	154
PfeI	GAWTC	1 cut(s)	155
PinAI	ACCGGT	1 cut(s)	756
PkrI	GCNGC	3 cut(s)	485, 649, 700
PpuMI	RGGWCCY	1 cut(s)	115
PshBI	ATTAAT	1 cut(s)	333
Psp5II	RGGWCCY	1 cut(s)	115
Psp6I	CCWGG	3 cut(s)	118, 691, 820
PspGI	CCWGG	3 cut(s)	118, 691, 820
PspN4I	GGNNCC	5 cut(s)	117, 146, 609, 819, 877
PspPI	GGNCC	4 cut(s)	115, 223, 817, 833
PspPPI	RGGWCCY	1 cut(s)	115
PstI	CTGCAG	1 cut(s)	703
PstNI	CAGNNNCTG	1 cut(s)	698
PsuI	RGATCY	1 cut(s)	607
RsaI	GTAC	1 cut(s)	591
RsaNI	GTAC	1 cut(s)	590
RseI	CAYNNNNRTG	1 cut(s)	333
SaqAI	TTAA	3 cut(s)	207, 333, 672
SatI	GCNGC	3 cut(s)	484, 648, 699
Sau3AI	GATC	5 cut(s)	16, 65, 72, 250, 607
Sau96I	GGNCC	4 cut(s)	115, 223, 817, 833
ScrFI	CCNGG	3 cut(s)	120, 693, 822
SfaNI	GCATC	1 cut(s)	562
SfcI	CTRYAG	2 cut(s)	129, 699
SinI	GGWCC	2 cut(s)	115, 817
SmiMI	CAYNNNNRTG	1 cut(s)	333
SmlI	CTYRAG	1 cut(s)	563
SmoI	CTYRAG	1 cut(s)	563
SphI	GCATGC	1 cut(s)	654
Sse9I	AATT	2 cut(s)	277, 705
SsiI	CCGC	3 cut(s)	90, 483, 654
SspI	AATATT	1 cut(s)	400
SspMI	CTAG	4 cut(s)	125, 410, 473, 477
StyD4I	CCNGG	3 cut(s)	118, 691, 820
StyI	CCWWGG	2 cut(s)	295, 603
TaaI	ACNGT	5 cut(s)	580, 589, 844, 868, 875
TaiI	ACGT	1 cut(s)	222
TaqI	TCGA	2 cut(s)	36, 378
TasI	AATT	2 cut(s)	277, 705
TatI	WGTACW	1 cut(s)	589
TauI	GCSGC	1 cut(s)	486
TfiI	GAWTC	1 cut(s)	155
Tru1I	TTAA	3 cut(s)	207, 333, 672
Tru9I	TTAA	3 cut(s)	207, 333, 672
TscAI	CASTG	1 cut(s)	849
TseFI	GTSAC	1 cut(s)	580
TseI	GCWGC	2 cut(s)	647, 698
Tsp45I	GTSAC	1 cut(s)	580
TspDTI	ATGAA	4 cut(s)	257, 285, 431, 796
TspRI	CASTG	1 cut(s)	849
VpaK11BI	GGWCC	2 cut(s)	115, 817
VspI	ATTAAT	1 cut(s)	333
XagI	CCTNNNNNAGG	1 cut(s)	230
XapI	RAATTY	1 cut(s)	277
XceI	RCATGY	1 cut(s)	654
XmiI	GTMKAC	1 cut(s)	870
XmnI	GAANNNNTTC	1 cut(s)	154
XspI	CTAG	4 cut(s)	125, 410, 473, 477

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.