Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0006330

Physical Location & Seq

Genomic Coordinates Forward (+)

20566295 .. 20569404

Gene Structure 3110 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g25270.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 900 bp

ATGGCGTCACAGTCAGAAGGTCAGGCAAAGATAATTGATGGCAAAACCATAGCACAGACTATCCGATCTGAAATTGCTGAGGAAGTTCACCATCTATCTCAGAAATATGGCAAGGTCCCTGGACTGGCAGTAGTTATTGTGGGGAGCAGGAAGGACTCCCAAAGCTATGTCAGCATGAAGCGAAAGGCATGTGCTGAAGTTGGCATTAAATCTGTGGACATAGATCTCCCTGAGAATGTGTCTCTAGATGATCTCATAGCCAAAGTTCACGAATTAAATGTGAATCCTGATGTACATGGCATACTTGTTCAGCTTCCGTTGCCAAGGCATATTAATGAAGAGAAAGTCTTAAGTGAAATCAGCATTGAGAAGGATGTAGATGGGTTTCATCCTCTCAACATCGGCAAGCTTGCAATGAAAGGCAGAGAACCTTTATTCCTCCCTTGCACTCCCAAGGGCTGTCTTGAACTTCTGTCGCGGAGTGGTATAAGCATAGCGGGAAAGAAGGCAGTGGTGGTGGGAAGAAGTAACATAGTTGGATTGCCAGTTTCATTGCTGCTTTTGAAAGCCAATGCTACTGTTACCATAGTTCATTCCAACACTTCTGATCCCGAGACTATCATTCGTGAAGCGGATATTATTATTGCTGCAGTAGGACAGGCAATGATGATCAAGGGTAGCTGGATAAAACCAGGTGCTGCAATCATAGATGTTGGCACAAATGCTGTGGATGACTCCAGCAAAAAGTCGGGTTATAGATTGGTCGGGGATGTAGATTTCCAAGAAGCATGTAAGGTAGCTGGATGGATAACTCCAGTTCCTGGTGGAGTTGGTCCGATGACTGTTGCAATGCTTCTCAAGAACACATTGGATGGTGCTAAGCGTGTGATTGCAGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004477 GO:0004488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006575 GO:0006725 GO:0006730 GO:0006732 GO:0006760 GO:0006807 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0016491 GO:0016645 GO:0016646 GO:0016787 GO:0016810 GO:0016814 GO:0019219 GO:0019222 GO:0019238 GO:0019752 GO:0031323 GO:0034641 GO:0042558 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0044030 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046653 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051186 GO:0055114 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

THF_DHG_CYH THF_DHG_CYH_C

Protein Analysis

300

Amino Acids

31.8

Weight (kDa)

7.05

Isoelectric Point (pI)

34.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
THF_DHG_CYH	PF00763	12 - 127	1.9e-42	Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain
THF_DHG_CYH_C	PF02882	157 - 301	1.5e-57	Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006330)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12290
fragaria_vesca	FvH4_2g25270 FvH4_2g25270 FvH4_2g25270 FvH4_2g25270
malus_domestica	MD11G1304100.v1.1
prunus_persica	Prupe.1G144700_v2.0.a1 Prupe.8G257500_v2.0.a1
pyrus_communis	pycom03g22520 pycom11g26580
rosa_chinensis	RchiOBHm_Chr3g0495451 RchiOBHm_Chr6g0293401
rosa_laevigata	RLG00000011949
rosa_multiflora	Rmu_sc0005599.1_g000023 Rmu_sc0006269.1_g000022
rosa_roxburghii	Rroxscaffold_7G00174180
rosa_rugosa	Rorug03G0273500 Rorug03G0273600 Rorug06G0235300 Rorug06G0235400 Rorug06G0235400
rosa_samantha	Rh3BG356400 Rh3CG353300 Rh3DG355500 Rh6CG362100
rosa_wichuraiana	Rw3G028050 Rw6G030290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	821
AccII	CGCG	1 cut(s)	478
AciI	CCGC	3 cut(s)	478, 497, 632
AclWI	GGATC	1 cut(s)	602
AcuI	CTGAAG	1 cut(s)	216
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	1 cut(s)	294
AfiI	CCNNNNNNNGG	2 cut(s)	124, 821
AflII	CTTAAG	1 cut(s)	349
AgsI	TTSAA	2 cut(s)	467, 565
AjnI	CCWGG	3 cut(s)	118, 691, 820
AloI	GAACNNNNNNTCC	2 cut(s)	420, 452
AluBI	AGCT	5 cut(s)	165, 313, 409, 681, 800
AluI	AGCT	5 cut(s)	165, 313, 409, 681, 800
Alw26I	GTCTC	2 cut(s)	246, 608
AlwI	GGATC	1 cut(s)	602
AlwNI	CAGNNNCTG	2 cut(s)	698, 821
Ama87I	CYCGRG	1 cut(s)	611
ApeKI	GCWGC	3 cut(s)	556, 647, 698
AseI	ATTAAT	1 cut(s)	333
AspS9I	GGNCC	2 cut(s)	115, 833
AsuHPI	GGTGA	1 cut(s)	80
AvaI	CYCGRG	1 cut(s)	611
AvaII	GGWCC	2 cut(s)	115, 833
BbvCI	CCTCAGC	1 cut(s)	78
BbvI	GCAGC	3 cut(s)	543, 634, 685
BccI	CCATC	5 cut(s)	32, 99, 374, 798, 866
BciT130I	CCWGG	3 cut(s)	120, 693, 822
BclI	TGATCA	1 cut(s)	669
BcoDI	GTCTC	2 cut(s)	246, 608
BfaI	CTAG	1 cut(s)	245
BfmI	CTRYAG	1 cut(s)	648
BfrI	CTTAAG	1 cut(s)	349
BglII	AGATCT	1 cut(s)	223
BisI	GCNGC	3 cut(s)	557, 648, 699
BlpI	GCTNAGC	1 cut(s)	879
BlsI	GCNGC	3 cut(s)	558, 649, 700
Bme1390I	CCNGG	3 cut(s)	120, 693, 822
Bme18I	GGWCC	2 cut(s)	115, 833
BmeT110I	CYCGRG	1 cut(s)	611
BmgT120I	GGNCC	2 cut(s)	115, 833
BmiI	GGNNCC	1 cut(s)	117
BmrFI	CCNGG	3 cut(s)	120, 693, 822
BpmI	CTGGAG	2 cut(s)	721, 798
Bpu10I	CCTNAGC	1 cut(s)	78
Bpu1102I	GCTNAGC	1 cut(s)	879
BpuEI	CTTGAG	1 cut(s)	842
BsaHI	GRCGYC	1 cut(s)	5
BsaJI	CCNNGG	3 cut(s)	118, 323, 453
Bsc4I	CCNNNNNNNGG	2 cut(s)	124, 821
Bse1I	ACTGG	3 cut(s)	129, 545, 815
Bse3DI	GCAATG	4 cut(s)	420, 551, 669, 855
BseBI	CCWGG	3 cut(s)	120, 693, 822
BseDI	CCNNGG	3 cut(s)	118, 323, 453
BseGI	GGATG	6 cut(s)	379, 388, 736, 775, 809, 877
BseLI	CCNNNNNNNGG	2 cut(s)	124, 821
BseMI	GCAATG	4 cut(s)	420, 551, 669, 855
BseMII	CTCAG	3 cut(s)	69, 113, 222
BseNI	ACTGG	3 cut(s)	129, 545, 815
BseXI	GCAGC	3 cut(s)	543, 634, 685
Bsh1236I	CGCG	1 cut(s)	478
BsiHKCI	CYCGRG	1 cut(s)	611
BslFI	GGGAC	1 cut(s)	101
BslI	CCNNNNNNNGG	2 cut(s)	124, 821
BsmAI	GTCTC	2 cut(s)	246, 608
BsmFI	GGGAC	1 cut(s)	101
BsoBI	CYCGRG	1 cut(s)	611
Bsp1407I	TGTACA	1 cut(s)	292
Bsp143I	GATC	5 cut(s)	65, 223, 250, 607, 669
Bsp1720I	GCTNAGC	1 cut(s)	879
BspACI	CCGC	3 cut(s)	478, 497, 632
BspCNI	CTCAG	3 cut(s)	70, 112, 223
BspFNI	CGCG	1 cut(s)	478
BspLI	GGNNCC	1 cut(s)	117
BspMAI	CTGCAG	1 cut(s)	652
BspPI	GGATC	1 cut(s)	602
BspTI	CTTAAG	1 cut(s)	349
BsrDI	GCAATG	4 cut(s)	420, 551, 669, 855
BsrGI	TGTACA	1 cut(s)	292
BsrI	ACTGG	3 cut(s)	129, 545, 815
BssECI	CCNNGG	3 cut(s)	118, 323, 453
BssMI	GATC	5 cut(s)	65, 223, 250, 607, 669
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	2 cut(s)	323, 453
Bst2UI	CCWGG	3 cut(s)	120, 693, 822
Bst4CI	ACNGT	3 cut(s)	12, 580, 844
Bst6I	CTCTTC	1 cut(s)	333
BstACI	GRCGYC	1 cut(s)	5
BstAFI	CTTAAG	1 cut(s)	349
BstAUI	TGTACA	1 cut(s)	292
BstC8I	GCNNGC	2 cut(s)	407, 411
BstDEI	CTNAG	4 cut(s)	78, 99, 231, 879
BstF5I	GGATG	6 cut(s)	379, 388, 736, 775, 809, 877
BstFNI	CGCG	1 cut(s)	478
BstKTI	GATC	5 cut(s)	68, 226, 253, 610, 672
BstMAI	GTCTC	2 cut(s)	246, 608
BstMBI	GATC	5 cut(s)	65, 223, 250, 607, 669
BstMWI	GCNNNNNNNGC	2 cut(s)	171, 319
BstNI	CCWGG	3 cut(s)	120, 693, 822
BstNSI	RCATGY	2 cut(s)	192, 792
BstSCI	CCNGG	3 cut(s)	118, 691, 820
BstSFI	CTRYAG	1 cut(s)	648
BstUI	CGCG	1 cut(s)	478
BstV1I	GCAGC	3 cut(s)	543, 634, 685
BstX2I	RGATCY	1 cut(s)	223
BstYI	RGATCY	1 cut(s)	223
BtsCI	GGATG	6 cut(s)	379, 388, 736, 775, 809, 877
BtsI	GCAGTG	1 cut(s)	516
BtsIMutI	CAGTG	1 cut(s)	516
Cac8I	GCNNGC	2 cut(s)	407, 411
CaiI	CAGNNNCTG	2 cut(s)	698, 821
Cfr13I	GGNCC	2 cut(s)	115, 833
CsiI	ACCWGGT	1 cut(s)	691
Csp6I	GTAC	1 cut(s)	293
CspCI	CAANNNNNGTGG	2 cut(s)	708, 743
CviAII	CATG	4 cut(s)	175, 189, 296, 789
CviJI	RGCY	8 cut(s)	165, 260, 313, 409, 459, 569, 681, 800
CviKI_1	RGCY	8 cut(s)	165, 260, 313, 409, 459, 569, 681, 800
CviQI	GTAC	1 cut(s)	293
DdeI	CTNAG	4 cut(s)	78, 99, 231, 879
DpnI	GATC	5 cut(s)	67, 225, 252, 609, 671
DpnII	GATC	5 cut(s)	65, 223, 250, 607, 669
Eam1104I	CTCTTC	1 cut(s)	333
EarI	CTCTTC	1 cut(s)	333
Eco130I	CCWWGG	2 cut(s)	323, 453
Eco47I	GGWCC	2 cut(s)	115, 833
Eco57I	CTGAAG	1 cut(s)	216
Eco88I	CYCGRG	1 cut(s)	611
EcoO109I	RGGNCCY	1 cut(s)	115
EcoRII	CCWGG	3 cut(s)	118, 691, 820
EcoT14I	CCWWGG	2 cut(s)	323, 453
ErhI	CCWWGG	2 cut(s)	323, 453
FaeI	CATG	4 cut(s)	178, 192, 299, 792
FaqI	GGGAC	1 cut(s)	101
FatI	CATG	4 cut(s)	174, 188, 295, 788
FauI	CCCGC	1 cut(s)	490
FbaI	TGATCA	1 cut(s)	669
Fnu4HI	GCNGC	3 cut(s)	557, 648, 699
FokI	GGATG	6 cut(s)	375, 386, 743, 782, 816, 884
Fsp4HI	GCNGC	3 cut(s)	557, 648, 699
FspBI	CTAG	1 cut(s)	245
GluI	GCNGC	3 cut(s)	557, 648, 699
GsuI	CTGGAG	2 cut(s)	721, 798
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	4 cut(s)	178, 192, 299, 792
HindIII	AAGCTT	1 cut(s)	407
HinfI	GANTC	3 cut(s)	155, 283, 734
HphI	GGTGA	1 cut(s)	80
Hpy166II	GTNNAC	3 cut(s)	88, 217, 268
Hpy188I	TCNGA	6 cut(s)	16, 65, 70, 102, 607, 837
Hpy188III	TCNNGA	7 cut(s)	245, 269, 287, 464, 611, 626, 859
Hpy8I	GTNNAC	3 cut(s)	88, 217, 268
HpyAV	CCTTC	4 cut(s)	11, 145, 364, 499
HpyCH4III	ACNGT	3 cut(s)	12, 580, 844
HpyCH4V	TGCA	6 cut(s)	413, 447, 650, 701, 848, 893
HpyF10VI	GCNNNNNNNGC	2 cut(s)	171, 319
HpyF3I	CTNAG	4 cut(s)	78, 99, 231, 879
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	4 cut(s)	178, 192, 299, 792
Ksp22I	TGATCA	1 cut(s)	669
Kzo9I	GATC	5 cut(s)	65, 223, 250, 607, 669
LmnI	GCTCC	1 cut(s)	144
Lsp1109I	GCAGC	3 cut(s)	543, 634, 685
MabI	ACCWGGT	1 cut(s)	691
MaeI	CTAG	1 cut(s)	245
MaeIII	GTNAC	3 cut(s)	6, 527, 580
MalI	GATC	5 cut(s)	67, 225, 252, 609, 671
MboI	GATC	5 cut(s)	65, 223, 250, 607, 669
MboII	GAAGA	2 cut(s)	350, 534
MflI	RGATCY	1 cut(s)	223
MluCI	AATT	3 cut(s)	33, 72, 272
MlyI	GAGTC	2 cut(s)	149, 728
MmeI	TCCRAC	2 cut(s)	517, 621
MnlI	CCTC	3 cut(s)	73, 402, 449
MseI	TTAA	4 cut(s)	207, 275, 333, 350
MslI	CAYNNNNRTG	1 cut(s)	333
MspCI	CTTAAG	1 cut(s)	349
MspR9I	CCNGG	3 cut(s)	120, 693, 822
MvaI	CCWGG	3 cut(s)	120, 693, 822
MvnI	CGCG	1 cut(s)	478
MwoI	GCNNNNNNNGC	2 cut(s)	171, 319
NdeII	GATC	5 cut(s)	65, 223, 250, 607, 669
NlaIII	CATG	4 cut(s)	178, 192, 299, 792
NlaIV	GGNNCC	1 cut(s)	117
NmuCI	GTSAC	1 cut(s)	6
NspI	RCATGY	2 cut(s)	192, 792
PfeI	GAWTC	1 cut(s)	283
PflMI	CCANNNNNTGG	1 cut(s)	821
PkrI	GCNGC	3 cut(s)	558, 649, 700
PleI	GAGTC	2 cut(s)	149, 728
PpsI	GAGTC	2 cut(s)	149, 728
PpuMI	RGGWCCY	1 cut(s)	115
PshBI	ATTAAT	1 cut(s)	333
Psp5II	RGGWCCY	1 cut(s)	115
Psp6I	CCWGG	3 cut(s)	118, 691, 820
PspGI	CCWGG	3 cut(s)	118, 691, 820
PspN4I	GGNNCC	1 cut(s)	117
PspPI	GGNCC	2 cut(s)	115, 833
PspPPI	RGGWCCY	1 cut(s)	115
PstI	CTGCAG	1 cut(s)	652
PstNI	CAGNNNCTG	2 cut(s)	698, 821
PsuI	RGATCY	1 cut(s)	223
RsaI	GTAC	1 cut(s)	294
RsaNI	GTAC	1 cut(s)	293
RseI	CAYNNNNRTG	1 cut(s)	333
SaqAI	TTAA	4 cut(s)	207, 275, 333, 350
SatI	GCNGC	3 cut(s)	557, 648, 699
Sau3AI	GATC	5 cut(s)	65, 223, 250, 607, 669
Sau96I	GGNCC	2 cut(s)	115, 833
SchI	GAGTC	2 cut(s)	149, 728
ScrFI	CCNGG	3 cut(s)	120, 693, 822
SexAI	ACCWGGT	1 cut(s)	691
SfcI	CTRYAG	1 cut(s)	648
SinI	GGWCC	2 cut(s)	115, 833
SmiMI	CAYNNNNRTG	1 cut(s)	333
SmlI	CTYRAG	2 cut(s)	349, 857
SmoI	CTYRAG	2 cut(s)	349, 857
Sse9I	AATT	3 cut(s)	33, 72, 272
SsiI	CCGC	3 cut(s)	478, 497, 632
SspMI	CTAG	1 cut(s)	245
StyD4I	CCNGG	3 cut(s)	118, 691, 820
StyI	CCWWGG	2 cut(s)	323, 453
TaaI	ACNGT	3 cut(s)	12, 580, 844
TasI	AATT	3 cut(s)	33, 72, 272
TatI	WGTACW	1 cut(s)	292
TfiI	GAWTC	1 cut(s)	283
Tru1I	TTAA	4 cut(s)	207, 275, 333, 350
Tru9I	TTAA	4 cut(s)	207, 275, 333, 350
TscAI	CASTG	1 cut(s)	516
TseFI	GTSAC	1 cut(s)	6
TseI	GCWGC	3 cut(s)	556, 647, 698
Tsp45I	GTSAC	1 cut(s)	6
TspDTI	ATGAA	6 cut(s)	191, 351, 377, 431, 540, 581
TspGWI	ACGGA	1 cut(s)	306
TspRI	CASTG	1 cut(s)	516
Van91I	CCANNNNNTGG	1 cut(s)	821
Vha464I	CTTAAG	1 cut(s)	349
VpaK11BI	GGWCC	2 cut(s)	115, 833
VspI	ATTAAT	1 cut(s)	333
XbaI	TCTAGA	1 cut(s)	244
XceI	RCATGY	2 cut(s)	192, 792
XspI	CTAG	1 cut(s)	245

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.