Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0010323

Physical Location & Seq

Genomic Coordinates Reverse (-)

3960401 .. 3961920

Gene Structure 1520 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G12490.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 477 bp

ATGGCTTTAGTCGGAGGTGTTGGCGATGTCCCTGCTAATCAGAACAGTGGTGAGGTTGAGAGCCTCGCTCGTTTCGCTGTCGATGAACACAACAAGAAAGAGAATGCACTTCTTGAGTTTGCGAGAGTTGTGAAGGCGAAAGAACAAGTTGTAGCCGGAACACTGCATCACCTGACTCTGGAGATTCTCGAGGCAGGACAGAAGAAGCTTTACGAAGCCAAAGTGTGGGTCAAGCCGTGGTTGAACTTTAAAGAGTTACAGGAGTTCAAGCCTGCCAGTGATGCCCCTGCTATCACTTCCTCCGATCTTGGCTGCAAGCAAGGTGAACATGAATCTGGATGGAGGGAAGTTCCCGGAGATGATCCAGAAGTGAAGCATGTTGCTGAGCAGGCTGTCAAGACCATTCAGCAGAGGTCTAACTCCTTGTTCCCTTATGAACTTCTGGAGGTTGTGCATGCTAAAGCTGAGTGTCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002020 GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005829 GO:0006950 GO:0006970 GO:0006972 GO:0006979 GO:0008150 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009892 GO:0010035 GO:0010466 GO:0010605 GO:0010951 GO:0012505 GO:0019222 GO:0019899 GO:0030162 GO:0030234 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0042221 GO:0043086 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044424 GO:0044444 GO:0044464 GO:0045861 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0050897 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0080090 GO:0098772 GO:1901700 GO:2000116 GO:2000117

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cystatin SQAPI

Protein Analysis

158

Amino Acids

17.48

Weight (kDa)

5.39

Isoelectric Point (pI)

32.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cystatin	PF00031	10 - 91	1.3e-16	Cystatin domain
SQAPI	PF16845	16 - 91	2.4e-20	Aspartic acid proteinase inhibitor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0010323)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12490 AT3G12490
fragaria_vesca	FvH4_7g04140
malus_domestica	MD02G1275000.v1.1 MD07G1043000.v1.1
prunus_persica	Prupe.2G046600_v2.0.a1 Prupe.2G047100_v2.0.a1
pyrus_communis	pycom02g23510
rosa_chinensis	RchiOBHm_Chr1g0328231
rosa_laevigata	RLG00000019109 RLG00000029935
rosa_multiflora	Rmu_sc0004646.1_g000040
rosa_roxburghii	Rroxscaffold_4G00321700
rosa_rugosa	Rorug01G0074400 Rorug01G0074500
rosa_samantha	Rh1AG091700 Rh1BG074000 Rh1CG088100 Rh1DG095100
rosa_wichuraiana	Rw0G011030 Rw1G007080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	225
AclWI	GGATC	1 cut(s)	356
AfiI	CCNNNNNNNGG	2 cut(s)	178, 225
AgsI	TTSAA	2 cut(s)	244, 268
AluBI	AGCT	2 cut(s)	208, 464
AluI	AGCT	2 cut(s)	208, 464
AlwI	GGATC	1 cut(s)	356
Ama87I	CYCGRG	1 cut(s)	188
ApeKI	GCWGC	1 cut(s)	312
ArsI	GACNNNNNNTTYG	2 cut(s)	213, 245
AsuC2I	CCSGG	1 cut(s)	354
AsuHPI	GGTGA	3 cut(s)	62, 161, 335
AvaI	CYCGRG	1 cut(s)	188
BarI	GAAGNNNNNNTAC	2 cut(s)	194, 226
BbvI	GCAGC	1 cut(s)	299
BccI	CCATC	1 cut(s)	333
BceAI	ACGGC	1 cut(s)	220
BcgI	CGANNNNNNTGC	2 cut(s)	14, 48
BcnI	CCSGG	1 cut(s)	354
BisI	GCNGC	1 cut(s)	313
BlpI	GCTNAGC	1 cut(s)	384
BlsI	GCNGC	1 cut(s)	314
Bme1390I	CCNGG	1 cut(s)	354
BmeT110I	CYCGRG	1 cut(s)	188
BmrFI	CCNGG	1 cut(s)	354
BmsI	GCATC	2 cut(s)	175, 271
BplI	GAGNNNNNCTC	2 cut(s)	52, 84
BpmI	CTGGAG	2 cut(s)	200, 464
Bpu1102I	GCTNAGC	1 cut(s)	384
BpuEI	CTTGAG	1 cut(s)	134
BpuMI	CCSGG	1 cut(s)	354
BsaJI	CCNNGG	1 cut(s)	236
Bsc4I	CCNNNNNNNGG	2 cut(s)	178, 225
Bse1I	ACTGG	1 cut(s)	276
BseDI	CCNNGG	1 cut(s)	236
BseGI	GGATG	1 cut(s)	344
BseLI	CCNNNNNNNGG	2 cut(s)	178, 225
BseMII	CTCAG	2 cut(s)	375, 456
BseNI	ACTGG	1 cut(s)	276
BseXI	GCAGC	1 cut(s)	299
BsiHKCI	CYCGRG	1 cut(s)	188
BsiSI	CCGG	2 cut(s)	156, 354
BslFI	GGGAC	1 cut(s)	14
BslI	CCNNNNNNNGG	2 cut(s)	178, 225
BsmFI	GGGAC	1 cut(s)	14
BsmI	GAATGC	1 cut(s)	109
BsoBI	CYCGRG	1 cut(s)	188
Bsp143I	GATC	2 cut(s)	304, 361
Bsp1720I	GCTNAGC	1 cut(s)	384
BspCNI	CTCAG	2 cut(s)	376, 457
BspPI	GGATC	1 cut(s)	356
BsrI	ACTGG	1 cut(s)	276
BssECI	CCNNGG	1 cut(s)	236
BssMI	GATC	2 cut(s)	304, 361
Bst4CI	ACNGT	1 cut(s)	47
BstC8I	GCNNGC	4 cut(s)	273, 317, 390, 456
BstDEI	CTNAG	2 cut(s)	384, 465
BstDSI	CCRYGG	1 cut(s)	236
BstF5I	GGATG	1 cut(s)	344
BstKTI	GATC	2 cut(s)	307, 364
BstMBI	GATC	2 cut(s)	304, 361
BstMWI	GCNNNNNNNGC	3 cut(s)	74, 281, 389
BstNSI	RCATGY	2 cut(s)	380, 458
BstSCI	CCNGG	1 cut(s)	352
BstV1I	GCAGC	1 cut(s)	299
BtgI	CCRYGG	1 cut(s)	236
BtgZI	GCGATG	1 cut(s)	39
BtsCI	GGATG	1 cut(s)	344
BtsI	GCAGTG	1 cut(s)	161
BtsIMutI	CAGTG	3 cut(s)	52, 161, 283
Cac8I	GCNNGC	4 cut(s)	273, 317, 390, 456
CviAII	CATG	3 cut(s)	329, 377, 455
DdeI	CTNAG	2 cut(s)	384, 465
DpnI	GATC	2 cut(s)	306, 363
DpnII	GATC	2 cut(s)	304, 361
DraI	TTTAAA	1 cut(s)	250
Eco88I	CYCGRG	1 cut(s)	188
FaeI	CATG	3 cut(s)	332, 380, 458
FaiI	YATR	4 cut(s)	330, 378, 435, 456
FaqI	GGGAC	1 cut(s)	14
FatI	CATG	3 cut(s)	328, 376, 454
Fnu4HI	GCNGC	1 cut(s)	313
FokI	GGATG	1 cut(s)	351
Fsp4HI	GCNGC	1 cut(s)	313
GluI	GCNGC	1 cut(s)	313
GsuI	CTGGAG	2 cut(s)	200, 464
HapII	CCGG	2 cut(s)	156, 354
Hin1II	CATG	3 cut(s)	332, 380, 458
HindIII	AAGCTT	1 cut(s)	206
HinfI	GANTC	3 cut(s)	175, 184, 332
HpaII	CCGG	2 cut(s)	156, 354
HphI	GGTGA	3 cut(s)	62, 161, 335
Hpy166II	GTNNAC	1 cut(s)	326
Hpy188I	TCNGA	3 cut(s)	14, 42, 304
Hpy188III	TCNNGA	7 cut(s)	113, 179, 188, 336, 365, 397, 443
Hpy8I	GTNNAC	1 cut(s)	326
HpyAV	CCTTC	1 cut(s)	127
HpyCH4III	ACNGT	1 cut(s)	47
HpyCH4V	TGCA	4 cut(s)	107, 166, 315, 454
HpyF10VI	GCNNNNNNNGC	3 cut(s)	74, 281, 389
HpyF3I	CTNAG	2 cut(s)	384, 465
Hsp92II	CATG	3 cut(s)	332, 380, 458
Kzo9I	GATC	2 cut(s)	304, 361
Lsp1109I	GCAGC	1 cut(s)	299
LweI	GCATC	2 cut(s)	175, 271
MaeIII	GTNAC	1 cut(s)	255
MalI	GATC	2 cut(s)	306, 363
MboI	GATC	2 cut(s)	304, 361
MboII	GAAGA	1 cut(s)	214
MlyI	GAGTC	1 cut(s)	169
MnlI	CCTC	8 cut(s)	8, 46, 74, 184, 310, 336, 405, 439
MseI	TTAA	1 cut(s)	249
MspI	CCGG	2 cut(s)	156, 354
MspR9I	CCNGG	1 cut(s)	354
Mva1269I	GAATGC	1 cut(s)	109
MwoI	GCNNNNNNNGC	3 cut(s)	74, 281, 389
NciI	CCSGG	1 cut(s)	354
NdeII	GATC	2 cut(s)	304, 361
NlaIII	CATG	3 cut(s)	332, 380, 458
NspI	RCATGY	2 cut(s)	380, 458
PaeI	GCATGC	1 cut(s)	458
PaeR7I	CTCGAG	1 cut(s)	188
PctI	GAATGC	1 cut(s)	109
PfeI	GAWTC	2 cut(s)	184, 332
PflMI	CCANNNNNTGG	1 cut(s)	225
PfoI	TCCNGGA	1 cut(s)	352
PkrI	GCNGC	1 cut(s)	314
PleI	GAGTC	1 cut(s)	169
PpsI	GAGTC	1 cut(s)	169
SaqAI	TTAA	1 cut(s)	249
SatI	GCNGC	1 cut(s)	313
Sau3AI	GATC	2 cut(s)	304, 361
SchI	GAGTC	1 cut(s)	169
ScrFI	CCNGG	1 cut(s)	354
SetI	ASST	8 cut(s)	19, 57, 174, 210, 325, 416, 450, 466
SfaNI	GCATC	2 cut(s)	175, 271
Sfr274I	CTCGAG	1 cut(s)	188
SlaI	CTCGAG	1 cut(s)	188
SmlI	CTYRAG	2 cut(s)	113, 188
SmoI	CTYRAG	2 cut(s)	113, 188
SphI	GCATGC	1 cut(s)	458
StyD4I	CCNGG	1 cut(s)	352
TaaI	ACNGT	1 cut(s)	47
TaqI	TCGA	2 cut(s)	81, 189
TfiI	GAWTC	2 cut(s)	184, 332
Tru1I	TTAA	1 cut(s)	249
Tru9I	TTAA	1 cut(s)	249
TscAI	CASTG	3 cut(s)	52, 168, 283
TseI	GCWGC	1 cut(s)	312
TspDTI	ATGAA	3 cut(s)	99, 345, 450
TspRI	CASTG	3 cut(s)	52, 168, 283
Van91I	CCANNNNNTGG	1 cut(s)	225
XceI	RCATGY	2 cut(s)	380, 458
XhoI	CTCGAG	1 cut(s)	188

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.