Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1B

Orthogroup ID

OG0010323

Physical Location & Seq

Genomic Coordinates Forward (+)

11439558 .. 11442143

Gene Structure 2586 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1BG074000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 717 bp

ATGAAATTGAAACCACAATCGAACTCATCGTCTTCCTCATTCTTCTCTCTCGTCCTCTTCTCCTTCATCGCACTCTCTTCGTCCTCTACAATGGCCACCCTCGGCGGAATTCGCGACTCCCCTGCCGGCTCCGAGAACAGCCTCGAGACCGAATCCCTCGGTCGCTTCGCCGTCGACGAGCATAACAAGAAGGAGAATGCTCTGCTGGAGTTTGTTCGAGTGGTGAAGGCGAAGGAACAGGTGGTTGCCGGCACTCTGCACCATCTGGTTGTGGAGGCCATCGACGGCGGCAAGAAGAAGCTTTATGAAGCCAAGGTCTGGGTCAAGCCTTGGTTGGGCTTTAAAGAAGTTCAGGAATTCAAGCACGCTGAGGATCCCGAAGCTGCATCTGAAACGCCTTCGTTTACTGCTTCCGATTTGGGCGTTAAGCAAGGTGGGCATGCTCCAGGGTGGCAAGATGTGCATCCTCATGATCCTCAAGTTCAAGATGCAGCCAACCATGCTGTGAAGAGCCTTCAGCAAAGGTCCAATTCGTTGTTCCCTTATGAGCTTCAAGAGGTTGTCCATGCTAAGGCAGAGGTGATAGAAGATCATGCCAAGTTCAACATGCTTCTTAAGCTGAAGAGGGGAGACAAAGAAGAGAAATATAGAGTTGAAGTGCACAAGAACAATGAAGGTGCCTACAATCTAAATCAGATGGAGGCACAGCACTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002020 GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005829 GO:0006950 GO:0006970 GO:0006972 GO:0006979 GO:0008150 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009892 GO:0010035 GO:0010466 GO:0010605 GO:0010951 GO:0012505 GO:0019222 GO:0019899 GO:0030162 GO:0030234 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0042221 GO:0043086 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044424 GO:0044444 GO:0044464 GO:0045861 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0050897 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0080090 GO:0098772 GO:1901700 GO:2000116 GO:2000117

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cystatin SQAPI

Protein Analysis

238

Amino Acids

26.45

Weight (kDa)

6.18

Isoelectric Point (pI)

40.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cystatin	PF00031	45 - 122	3.4e-14	Cystatin domain
SQAPI	PF16845	51 - 122	1.7e-19	Aspartic acid proteinase inhibitor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010323)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12490 AT3G12490
fragaria_vesca	FvH4_7g04140
malus_domestica	MD02G1275000.v1.1 MD07G1043000.v1.1
prunus_persica	Prupe.2G046600_v2.0.a1 Prupe.2G047100_v2.0.a1
pyrus_communis	pycom02g23510
rosa_chinensis	RchiOBHm_Chr1g0328231
rosa_laevigata	RLG00000019109 RLG00000029935
rosa_multiflora	Rmu_sc0004646.1_g000040
rosa_roxburghii	Rroxscaffold_4G00321700
rosa_rugosa	Rorug01G0074400 Rorug01G0074500
rosa_samantha	Rh1AG091700 Rh1BG074000 Rh1CG088100 Rh1DG095100
rosa_wichuraiana	Rw0G011030 Rw1G007080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	677
AccB7I	CCANNNNNTGG	1 cut(s)	318
AccI	GTMKAC	1 cut(s)	174
AccII	CGCG	1 cut(s)	114
AciI	CCGC	2 cut(s)	105, 288
AclWI	GGATC	3 cut(s)	368, 381, 467
AcoI	YGGCCR	1 cut(s)	93
AcsI	RAATTY	2 cut(s)	108, 356
AcuI	CTGAAG	2 cut(s)	500, 641
AfiI	CCNNNNNNNGG	3 cut(s)	318, 335, 571
AflII	CTTAAG	1 cut(s)	614
AgsI	TTSAA	6 cut(s)	10, 361, 485, 554, 604, 656
AjnI	CCWGG	1 cut(s)	445
AjuI	GAANNNNNNNTTGG	2 cut(s)	521, 553
AluBI	AGCT	4 cut(s)	301, 383, 550, 619
AluI	AGCT	4 cut(s)	301, 383, 550, 619
Alw21I	GWGCWC	1 cut(s)	663
Alw26I	GTCTC	2 cut(s)	140, 624
Alw44I	GTGCAC	1 cut(s)	659
AlwI	GGATC	3 cut(s)	368, 381, 467
Ama87I	CYCGRG	1 cut(s)	143
AoxI	GGCC	2 cut(s)	93, 276
ApaLI	GTGCAC	1 cut(s)	659
ApeKI	GCWGC	2 cut(s)	383, 491
ApoI	RAATTY	2 cut(s)	108, 356
ArsI	GACNNNNNNTTYG	2 cut(s)	14, 46
AspS9I	GGNCC	1 cut(s)	525
AsuHPI	GGTGA	2 cut(s)	235, 592
AvaI	CYCGRG	1 cut(s)	143
AvaII	GGWCC	1 cut(s)	525
BaeGI	GKGCMC	1 cut(s)	663
BalI	TGGCCA	1 cut(s)	95
BamHI	GGATCC	1 cut(s)	373
BanI	GGYRCC	1 cut(s)	677
BbsI	GAAGAC	1 cut(s)	24
Bbv12I	GWGCWC	1 cut(s)	663
BbvCI	CCTCAGC	1 cut(s)	369
BbvI	GCAGC	2 cut(s)	370, 503
BccI	CCATC	3 cut(s)	270, 287, 691
BceAI	ACGGC	2 cut(s)	155, 301
BcgI	CGANNNNNNTGC	4 cut(s)	60, 94, 104, 138
BciT130I	CCWGG	1 cut(s)	447
BcoDI	GTCTC	2 cut(s)	140, 624
BfrI	CTTAAG	1 cut(s)	614
BisI	GCNGC	3 cut(s)	289, 384, 492
BlsI	GCNGC	3 cut(s)	290, 385, 493
Bme1390I	CCNGG	1 cut(s)	447
Bme18I	GGWCC	1 cut(s)	525
BmeT110I	CYCGRG	1 cut(s)	143
BmgT120I	GGNCC	1 cut(s)	525
BmiI	GGNNCC	3 cut(s)	130, 375, 679
BmrFI	CCNGG	1 cut(s)	447
BmsI	GCATC	3 cut(s)	395, 472, 478
BpiI	GAAGAC	1 cut(s)	24
BpmI	CTGGAG	2 cut(s)	227, 429
Bpu10I	CCTNAGC	2 cut(s)	369, 570
BpuEI	CTTGAG	1 cut(s)	462
BsaBI	GATNNNNATC	1 cut(s)	462
BsaI	GGTCTC	1 cut(s)	140
BsaJI	CCNNGG	5 cut(s)	100, 157, 312, 329, 446
Bsc4I	CCNNNNNNNGG	3 cut(s)	318, 335, 571
Bse118I	RCCGGY	2 cut(s)	125, 248
Bse8I	GATNNNNATC	1 cut(s)	462
BseBI	CCWGG	1 cut(s)	447
BseDI	CCNNGG	5 cut(s)	100, 157, 312, 329, 446
BseGI	GGATG	1 cut(s)	463
BseJI	GATNNNNATC	1 cut(s)	462
BseLI	CCNNNNNNNGG	3 cut(s)	318, 335, 571
BseMII	CTCAG	1 cut(s)	360
BseSI	GKGCMC	1 cut(s)	663
BseXI	GCAGC	2 cut(s)	370, 503
BsgI	GTGCAG	1 cut(s)	242
Bsh1236I	CGCG	1 cut(s)	114
Bsh1285I	CGRYCG	1 cut(s)	163
BshFI	GGCC	2 cut(s)	95, 278
BshNI	GGYRCC	1 cut(s)	677
BsiEI	CGRYCG	1 cut(s)	163
BsiHKAI	GWGCWC	1 cut(s)	663
BsiHKCI	CYCGRG	1 cut(s)	143
BsiSI	CCGG	2 cut(s)	126, 249
BslI	CCNNNNNNNGG	3 cut(s)	318, 335, 571
BsmAI	GTCTC	2 cut(s)	140, 624
BsmI	GAATGC	1 cut(s)	202
BsnI	GGCC	2 cut(s)	95, 278
Bso31I	GGTCTC	1 cut(s)	140
BsoBI	CYCGRG	1 cut(s)	143
Bsp1286I	GDGCHC	1 cut(s)	663
Bsp143I	GATC	3 cut(s)	373, 472, 589
Bsp68I	TCGCGA	1 cut(s)	114
BspACI	CCGC	2 cut(s)	105, 288
BspANI	GGCC	2 cut(s)	95, 278
BspCNI	CTCAG	1 cut(s)	361
BspFNI	CGCG	1 cut(s)	114
BspHI	TCATGA	1 cut(s)	469
BspLI	GGNNCC	3 cut(s)	130, 375, 679
BspPI	GGATC	3 cut(s)	368, 381, 467
BspQI	GCTCTTC	1 cut(s)	503
BspT107I	GGYRCC	1 cut(s)	677
BspTI	CTTAAG	1 cut(s)	614
BspTNI	GGTCTC	1 cut(s)	140
BsrFI	RCCGGY	2 cut(s)	125, 248
BssAI	RCCGGY	2 cut(s)	125, 248
BssECI	CCNNGG	5 cut(s)	100, 157, 312, 329, 446
BssMI	GATC	3 cut(s)	373, 472, 589
BssT1I	CCWWGG	2 cut(s)	312, 329
Bst2UI	CCWGG	1 cut(s)	447
Bst6I	CTCTTC	5 cut(s)	62, 82, 503, 617, 633
BstAFI	CTTAAG	1 cut(s)	614
BstAPI	GCANNNNNTGC	1 cut(s)	460
BstC8I	GCNNGC	4 cut(s)	127, 250, 366, 441
BstDEI	CTNAG	2 cut(s)	369, 570
BstF5I	GGATG	1 cut(s)	463
BstFNI	CGCG	1 cut(s)	114
BstKTI	GATC	3 cut(s)	376, 475, 592
BstMAI	GTCTC	2 cut(s)	140, 624
BstMBI	GATC	3 cut(s)	373, 472, 589
BstMCI	CGRYCG	1 cut(s)	163
BstMWI	GCNNNNNNNGC	5 cut(s)	111, 436, 460, 500, 616
BstNI	CCWGG	1 cut(s)	447
BstNSI	RCATGY	2 cut(s)	443, 610
BstSCI	CCNGG	1 cut(s)	445
BstSLI	GKGCMC	1 cut(s)	663
BstUI	CGCG	1 cut(s)	114
BstV1I	GCAGC	2 cut(s)	370, 503
BstV2I	GAAGAC	1 cut(s)	24
BstX2I	RGATCY	1 cut(s)	373
BstYI	RGATCY	1 cut(s)	373
BsuRI	GGCC	2 cut(s)	95, 278
BtgZI	GCGATG	1 cut(s)	52
BtsCI	GGATG	1 cut(s)	463
BtuMI	TCGCGA	1 cut(s)	114
Cac8I	GCNNGC	4 cut(s)	127, 250, 366, 441
CciI	TCATGA	1 cut(s)	469
Cfr10I	RCCGGY	2 cut(s)	125, 248
Cfr13I	GGNCC	1 cut(s)	525
CviAII	CATG	6 cut(s)	440, 470, 500, 566, 593, 607
DdeI	CTNAG	2 cut(s)	369, 570
DpnI	GATC	3 cut(s)	375, 474, 591
DpnII	GATC	3 cut(s)	373, 472, 589
DraI	TTTAAA	1 cut(s)	343
EaeI	YGGCCR	1 cut(s)	93
Eam1104I	CTCTTC	5 cut(s)	62, 82, 503, 617, 633
EarI	CTCTTC	5 cut(s)	62, 82, 503, 617, 633
EciI	GGCGGA	1 cut(s)	120
Eco130I	CCWWGG	2 cut(s)	312, 329
Eco31I	GGTCTC	1 cut(s)	140
Eco47I	GGWCC	1 cut(s)	525
Eco57I	CTGAAG	2 cut(s)	500, 641
Eco88I	CYCGRG	1 cut(s)	143
EcoRI	GAATTC	2 cut(s)	108, 356
EcoRII	CCWGG	1 cut(s)	445
EcoT14I	CCWWGG	2 cut(s)	312, 329
ErhI	CCWWGG	2 cut(s)	312, 329
FaeI	CATG	6 cut(s)	443, 473, 503, 569, 596, 610
FatI	CATG	6 cut(s)	439, 469, 499, 565, 592, 606
FblI	GTMKAC	1 cut(s)	174
Fnu4HI	GCNGC	3 cut(s)	289, 384, 492
FokI	GGATG	1 cut(s)	450
Fsp4HI	GCNGC	3 cut(s)	289, 384, 492
GluI	GCNGC	3 cut(s)	289, 384, 492
GsuI	CTGGAG	2 cut(s)	227, 429
HaeIII	GGCC	2 cut(s)	95, 278
HapII	CCGG	2 cut(s)	126, 249
Hin1II	CATG	6 cut(s)	443, 473, 503, 569, 596, 610
HincII	GTYRAC	1 cut(s)	175
HindII	GTYRAC	1 cut(s)	175
HindIII	AAGCTT	1 cut(s)	299
HinfI	GANTC	2 cut(s)	116, 152
HpaII	CCGG	2 cut(s)	126, 249
HphI	GGTGA	2 cut(s)	235, 592
Hpy166II	GTNNAC	3 cut(s)	175, 405, 661
Hpy188I	TCNGA	4 cut(s)	133, 391, 415, 696
Hpy188III	TCNNGA	7 cut(s)	113, 145, 353, 377, 470, 485, 554
Hpy8I	GTNNAC	3 cut(s)	175, 405, 661
Hpy99I	CGWCG	3 cut(s)	176, 179, 287
HpyAV	CCTTC	7 cut(s)	73, 184, 220, 226, 408, 524, 668
HpyCH4V	TGCA	5 cut(s)	259, 386, 463, 491, 661
HpyF10VI	GCNNNNNNNGC	5 cut(s)	111, 436, 460, 500, 616
HpyF3I	CTNAG	2 cut(s)	369, 570
Hsp92II	CATG	6 cut(s)	443, 473, 503, 569, 596, 610
KroI	GCCGGC	2 cut(s)	125, 248
KroNI	GCCGGC	2 cut(s)	127, 250
Kzo9I	GATC	3 cut(s)	373, 472, 589
LguI	GCTCTTC	1 cut(s)	503
LmnI	GCTCC	2 cut(s)	134, 448
Lsp1109I	GCAGC	2 cut(s)	370, 503
LweI	GCATC	3 cut(s)	395, 472, 478
MalI	GATC	3 cut(s)	375, 474, 591
MboI	GATC	3 cut(s)	373, 472, 589
MboII	GAAGA	9 cut(s)	24, 34, 49, 69, 307, 520, 599, 634, 650
MflI	RGATCY	1 cut(s)	373
MhlI	GDGCHC	1 cut(s)	663
MlsI	TGGCCA	1 cut(s)	95
MluCI	AATT	4 cut(s)	5, 108, 356, 529
MluNI	TGGCCA	1 cut(s)	95
MlyI	GAGTC	1 cut(s)	110
Mox20I	TGGCCA	1 cut(s)	95
MroNI	GCCGGC	2 cut(s)	125, 248
MscI	TGGCCA	1 cut(s)	95
MseI	TTAA	4 cut(s)	342, 426, 615, 715
MslI	CAYNNNNRTG	1 cut(s)	468
Msp20I	TGGCCA	1 cut(s)	95
MspCI	CTTAAG	1 cut(s)	614
MspI	CCGG	2 cut(s)	126, 249
MspR9I	CCNGG	1 cut(s)	447
Mva1269I	GAATGC	1 cut(s)	202
MvaI	CCWGG	1 cut(s)	447
MvnI	CGCG	1 cut(s)	114
MwoI	GCNNNNNNNGC	5 cut(s)	111, 436, 460, 500, 616
NaeI	GCCGGC	2 cut(s)	127, 250
NdeII	GATC	3 cut(s)	373, 472, 589
NgoMIV	GCCGGC	2 cut(s)	125, 248
NlaIII	CATG	6 cut(s)	443, 473, 503, 569, 596, 610
NlaIV	GGNNCC	3 cut(s)	130, 375, 679
NmeAIII	GCCGAG	1 cut(s)	81
NruI	TCGCGA	1 cut(s)	114
NspI	RCATGY	2 cut(s)	443, 610
PaeI	GCATGC	1 cut(s)	443
PaeR7I	CTCGAG	1 cut(s)	143
PagI	TCATGA	1 cut(s)	469
PciSI	GCTCTTC	1 cut(s)	503
PcsI	WCGNNNNNNNCGW	2 cut(s)	26, 174
PctI	GAATGC	1 cut(s)	202
PdiI	GCCGGC	2 cut(s)	127, 250
PfeI	GAWTC	1 cut(s)	152
PflMI	CCANNNNNTGG	1 cut(s)	318
PkrI	GCNGC	3 cut(s)	290, 385, 493
PleI	GAGTC	1 cut(s)	110
PpsI	GAGTC	1 cut(s)	110
Psp6I	CCWGG	1 cut(s)	445
PspGI	CCWGG	1 cut(s)	445
PspN4I	GGNNCC	3 cut(s)	130, 375, 679
PspPI	GGNCC	1 cut(s)	525
PsuI	RGATCY	1 cut(s)	373
RruI	TCGCGA	1 cut(s)	114
RseI	CAYNNNNRTG	1 cut(s)	468
SalI	GTCGAC	1 cut(s)	173
SapI	GCTCTTC	1 cut(s)	503
SaqAI	TTAA	4 cut(s)	342, 426, 615, 715
SatI	GCNGC	3 cut(s)	289, 384, 492
Sau3AI	GATC	3 cut(s)	373, 472, 589
Sau96I	GGNCC	1 cut(s)	525
SchI	GAGTC	1 cut(s)	110
ScrFI	CCNGG	1 cut(s)	447
SduI	GDGCHC	1 cut(s)	663
SfaNI	GCATC	3 cut(s)	395, 472, 478
Sfr274I	CTCGAG	1 cut(s)	143
SgrDI	CGTCGACG	1 cut(s)	173
SinI	GGWCC	1 cut(s)	525
SlaI	CTCGAG	1 cut(s)	143
SmiMI	CAYNNNNRTG	1 cut(s)	468
SmlI	CTYRAG	3 cut(s)	143, 477, 614
SmoI	CTYRAG	3 cut(s)	143, 477, 614
SphI	GCATGC	1 cut(s)	443
Sse9I	AATT	4 cut(s)	5, 108, 356, 529
SsiI	CCGC	2 cut(s)	105, 288
StyD4I	CCNGG	1 cut(s)	445
StyI	CCWWGG	2 cut(s)	312, 329
TaqI	TCGA	5 cut(s)	20, 144, 174, 217, 282
TaqII	GACCGA	2 cut(s)	149, 164
TasI	AATT	4 cut(s)	5, 108, 356, 529
TauI	GCSGC	1 cut(s)	291
TfiI	GAWTC	1 cut(s)	152
Tru1I	TTAA	4 cut(s)	342, 426, 615, 715
Tru9I	TTAA	4 cut(s)	342, 426, 615, 715
TseI	GCWGC	2 cut(s)	383, 491
TspDTI	ATGAA	4 cut(s)	17, 55, 321, 687
Van91I	CCANNNNNTGG	1 cut(s)	318
Vha464I	CTTAAG	1 cut(s)	614
VneI	GTGCAC	1 cut(s)	659
VpaK11BI	GGWCC	1 cut(s)	525
XapI	RAATTY	2 cut(s)	108, 356
XceI	RCATGY	2 cut(s)	443, 610
XhoI	CTCGAG	1 cut(s)	143
XmiI	GTMKAC	1 cut(s)	174

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.