Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0010323

Physical Location & Seq

Genomic Coordinates Reverse (-)

3598980 .. 3601045

Gene Structure 2066 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1043000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 744 bp

ATGAAATCGGAATCTTACCTCTCTCTATTAATTGCTCCCTTCTTCCTCCTCCTCCTCCTCCTCGCTTTCCCTTCCACTACCGCACAAAAATCAACCCGAATTGGCTGCGGCCACAGTTCCCAACACCGGAAAATGGACACAGTCGGCGGAATCCAAGAATCCCGCGGCGTCCAGAACAGCGCCGAAGTTGAAGACCTCGCTCGCTTCGCCGTCCAAGAGCACAATAACAAGGAGAATGCCCTGCTCGAATTTGTGAGGGTTGTGAAGGCGAAAGAGCAGGTGGTTGCCGGAACTCTGCACCATCTGACGATCGAGGCGATCGAGGCCGGCAAGAAGAAGCTGTACGAAGCGAAGGTGTGGGTGAAGCCTTGGATGGGCTTCAAAGAAGAGCAGGAGTTCAAGCAGGCTGATGAGGAGGAGACACCATCCGTTACCTCTTCCGATCTTGGTGCCAAGCAAGGTGGGCATCCTCCTGGGTGGCAATCTGTGCCGCCGCATGACCCGCAGGTGCAGGATGCGGCTAACCACGCTGTTAAGTCCCTCCAGCAGAAGTCTAATTCCTTGTTGCCTTATGAGCTTCTAGAAGTTGTTCACGCCAAGGCTGAGGTGATAGAAGAGCATGCAAAGTTTAACATGCTTCTGAAGGTGAAAAGGGGAAGCAAAGAAGAGAAGTTTAAAGCTGAAGTGCATAAGAATATGGAAGGCGCCTTCAGTCTGAATCATATGGAGGCAGACCACTCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002020 GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005829 GO:0006950 GO:0006970 GO:0006972 GO:0006979 GO:0008150 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009892 GO:0010035 GO:0010466 GO:0010605 GO:0010951 GO:0012505 GO:0019222 GO:0019899 GO:0030162 GO:0030234 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0042221 GO:0043086 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044424 GO:0044444 GO:0044464 GO:0045861 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0050897 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0080090 GO:0098772 GO:1901700 GO:2000116 GO:2000117

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cystatin SQAPI

Protein Analysis

248

Amino Acids

27.58

Weight (kDa)

6.36

Isoelectric Point (pI)

43.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cystatin	PF00031	49 - 135	8.9e-17	Cystatin domain
SQAPI	PF16845	61 - 135	1.2e-18	Aspartic acid proteinase inhibitor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010323)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12490 AT3G12490
fragaria_vesca	FvH4_7g04140
malus_domestica	MD02G1275000.v1.1 MD07G1043000.v1.1
prunus_persica	Prupe.2G046600_v2.0.a1 Prupe.2G047100_v2.0.a1
pyrus_communis	pycom02g23510
rosa_chinensis	RchiOBHm_Chr1g0328231
rosa_laevigata	RLG00000019109 RLG00000029935
rosa_multiflora	Rmu_sc0004646.1_g000040
rosa_roxburghii	Rroxscaffold_4G00321700
rosa_rugosa	Rorug01G0074400 Rorug01G0074500
rosa_samantha	Rh1AG091700 Rh1BG074000 Rh1CG088100 Rh1DG095100
rosa_wichuraiana	Rw0G011030 Rw1G007080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	268, 496
Acc36I	ACCTGC	2 cut(s)	268, 496
AccB1I	GGYRCC	2 cut(s)	449, 704
AccII	CGCG	1 cut(s)	165
AciI	CCGC	9 cut(s)	81, 108, 147, 163, 165, 491, 494, 503, 518
AcoI	YGGCCR	1 cut(s)	109
AcsI	RAATTY	1 cut(s)	248
AcuI	CTGAAG	3 cut(s)	662, 694, 702
AcyI	GRCGYC	2 cut(s)	168, 705
AfaI	GTAC	1 cut(s)	344
AfiI	CCNNNNNNNGG	3 cut(s)	126, 133, 374
AgsI	TTSAA	3 cut(s)	191, 382, 400
AjnI	CCWGG	1 cut(s)	472
AluBI	AGCT	3 cut(s)	340, 577, 680
AluI	AGCT	3 cut(s)	340, 577, 680
Alw21I	GWGCWC	1 cut(s)	222
Alw26I	GTCTC	1 cut(s)	413
AoxI	GGCC	2 cut(s)	109, 324
ApeKI	GCWGC	1 cut(s)	105
ApoI	RAATTY	1 cut(s)	248
AseI	ATTAAT	1 cut(s)	29
Asp700I	GAANNNNTTC	1 cut(s)	588
AspLEI	GCGC	2 cut(s)	182, 707
AsuHPI	GGTGA	3 cut(s)	373, 619, 658
BanI	GGYRCC	2 cut(s)	449, 704
BarI	GAAGNNNNNNTAC	2 cut(s)	326, 358
BbsI	GAAGAC	1 cut(s)	198
Bbv12I	GWGCWC	1 cut(s)	222
BbvCI	CCTCAGC	1 cut(s)	603
BbvI	GCAGC	1 cut(s)	92
BccI	CCATC	3 cut(s)	309, 367, 433
BceAI	ACGGC	1 cut(s)	194
BcgI	CGANNNNNNTGC	4 cut(s)	87, 121, 431, 465
BciT130I	CCWGG	1 cut(s)	474
BcoDI	GTCTC	1 cut(s)	413
BfaI	CTAG	1 cut(s)	581
BfoI	RGCGCY	2 cut(s)	183, 708
BfuAI	ACCTGC	2 cut(s)	268, 496
BisI	GCNGC	6 cut(s)	106, 109, 166, 491, 494, 519
BlsI	GCNGC	6 cut(s)	107, 110, 167, 492, 495, 520
Bme1390I	CCNGG	1 cut(s)	474
BmiI	GGNNCC	2 cut(s)	451, 706
BmrFI	CCNGG	1 cut(s)	474
BmsI	GCATC	2 cut(s)	475, 505
BpiI	GAAGAC	1 cut(s)	198
BpmI	CTGGAG	1 cut(s)	527
Bpu10I	CCTNAGC	1 cut(s)	603
BsaHI	GRCGYC	2 cut(s)	168, 705
BsaJI	CCNNGG	4 cut(s)	163, 368, 473, 597
BsaWI	WCCGGW	1 cut(s)	126
Bsc4I	CCNNNNNNNGG	3 cut(s)	126, 133, 374
Bse118I	RCCGGY	1 cut(s)	326
BseBI	CCWGG	1 cut(s)	474
BseDI	CCNNGG	4 cut(s)	163, 368, 473, 597
BseGI	GGATG	4 cut(s)	378, 425, 466, 520
BseLI	CCNNNNNNNGG	3 cut(s)	126, 133, 374
BseMII	CTCAG	1 cut(s)	594
BseRI	GAGGAG	7 cut(s)	38, 41, 44, 47, 50, 428, 431
BseXI	GCAGC	1 cut(s)	92
BsgI	GTGCAG	2 cut(s)	281, 530
Bsh1236I	CGCG	1 cut(s)	165
Bsh1285I	CGRYCG	2 cut(s)	312, 321
BshFI	GGCC	2 cut(s)	111, 326
BshNI	GGYRCC	2 cut(s)	449, 704
BsiEI	CGRYCG	2 cut(s)	312, 321
BsiHKAI	GWGCWC	1 cut(s)	222
BsiSI	CCGG	3 cut(s)	127, 288, 327
BslFI	GGGAC	1 cut(s)	523
BslI	CCNNNNNNNGG	3 cut(s)	126, 133, 374
BsmAI	GTCTC	1 cut(s)	413
BsmFI	GGGAC	1 cut(s)	523
BsmI	GAATGC	1 cut(s)	241
BsnI	GGCC	2 cut(s)	111, 326
Bsp1286I	GDGCHC	1 cut(s)	222
Bsp143I	GATC	3 cut(s)	309, 318, 442
BspACI	CCGC	9 cut(s)	81, 108, 147, 163, 165, 491, 494, 503, 518
BspANI	GGCC	2 cut(s)	111, 326
BspCNI	CTCAG	1 cut(s)	595
BspFNI	CGCG	1 cut(s)	165
BspLI	GGNNCC	2 cut(s)	451, 706
BspMI	ACCTGC	2 cut(s)	268, 496
BspQI	GCTCTTC	2 cut(s)	381, 609
BspT107I	GGYRCC	2 cut(s)	449, 704
BsrFI	RCCGGY	1 cut(s)	326
BssAI	RCCGGY	1 cut(s)	326
BssECI	CCNNGG	4 cut(s)	163, 368, 473, 597
BssMI	GATC	3 cut(s)	309, 318, 442
BssNI	GRCGYC	2 cut(s)	168, 705
BssT1I	CCWWGG	2 cut(s)	368, 597
Bst2UI	CCWGG	1 cut(s)	474
Bst4CI	ACNGT	2 cut(s)	116, 142
Bst6I	CTCTTC	4 cut(s)	381, 442, 609, 660
BstACI	GRCGYC	2 cut(s)	168, 705
BstAPI	GCANNNNNTGC	1 cut(s)	487
BstC8I	GCNNGC	4 cut(s)	202, 328, 405, 621
BstDEI	CTNAG	1 cut(s)	603
BstDSI	CCRYGG	1 cut(s)	163
BstF5I	GGATG	4 cut(s)	378, 425, 466, 520
BstFNI	CGCG	1 cut(s)	165
BstH2I	RGCGCY	2 cut(s)	183, 708
BstHHI	GCGC	2 cut(s)	182, 707
BstKTI	GATC	3 cut(s)	312, 321, 445
BstMAI	GTCTC	1 cut(s)	413
BstMBI	GATC	3 cut(s)	309, 318, 442
BstMCI	CGRYCG	2 cut(s)	312, 321
BstMWI	GCNNNNNNNGC	7 cut(s)	206, 323, 463, 487, 502, 527, 574
BstNI	CCWGG	1 cut(s)	474
BstNSI	RCATGY	2 cut(s)	623, 637
BstSCI	CCNGG	1 cut(s)	472
BstUI	CGCG	1 cut(s)	165
BstV1I	GCAGC	1 cut(s)	92
BstV2I	GAAGAC	1 cut(s)	198
BsuRI	GGCC	2 cut(s)	111, 326
BtgI	CCRYGG	1 cut(s)	163
BtsCI	GGATG	4 cut(s)	378, 425, 466, 520
BveI	ACCTGC	2 cut(s)	268, 496
Cac8I	GCNNGC	4 cut(s)	202, 328, 405, 621
CfoI	GCGC	2 cut(s)	182, 707
Cfr10I	RCCGGY	1 cut(s)	326
Cfr42I	CCGCGG	1 cut(s)	166
CseI	GACGC	1 cut(s)	157
Csp6I	GTAC	1 cut(s)	343
CspCI	CAANNNNNGTGG	2 cut(s)	442, 477
CviAII	CATG	3 cut(s)	497, 620, 634
CviQI	GTAC	1 cut(s)	343
DdeI	CTNAG	1 cut(s)	603
DinI	GGCGCC	1 cut(s)	706
DpnI	GATC	3 cut(s)	311, 320, 444
DpnII	GATC	3 cut(s)	309, 318, 442
DraI	TTTAAA	1 cut(s)	676
EaeI	YGGCCR	1 cut(s)	109
Eam1104I	CTCTTC	4 cut(s)	381, 442, 609, 660
EarI	CTCTTC	4 cut(s)	381, 442, 609, 660
EciI	GGCGGA	1 cut(s)	162
Eco130I	CCWWGG	2 cut(s)	368, 597
Eco57I	CTGAAG	3 cut(s)	662, 694, 702
EcoRII	CCWGG	1 cut(s)	472
EcoT14I	CCWWGG	2 cut(s)	368, 597
EgeI	GGCGCC	1 cut(s)	706
EheI	GGCGCC	1 cut(s)	706
ErhI	CCWWGG	2 cut(s)	368, 597
FaeI	CATG	3 cut(s)	500, 623, 637
FaiI	YATR	8 cut(s)	498, 573, 621, 635, 690, 698, 723, 725
FaqI	GGGAC	1 cut(s)	523
FatI	CATG	3 cut(s)	496, 619, 633
FauI	CCCGC	2 cut(s)	170, 510
FauNDI	CATATG	1 cut(s)	723
Fnu4HI	GCNGC	6 cut(s)	106, 109, 166, 491, 494, 519
FokI	GGATG	4 cut(s)	385, 412, 453, 527
Fsp4HI	GCNGC	6 cut(s)	106, 109, 166, 491, 494, 519
FspBI	CTAG	1 cut(s)	581
GlaI	GCGC	2 cut(s)	181, 706
GluI	GCNGC	6 cut(s)	106, 109, 166, 491, 494, 519
GsuI	CTGGAG	1 cut(s)	527
HaeII	RGCGCY	2 cut(s)	183, 708
HaeIII	GGCC	2 cut(s)	111, 326
HapII	CCGG	3 cut(s)	127, 288, 327
HgaI	GACGC	1 cut(s)	157
HhaI	GCGC	2 cut(s)	182, 707
Hin1I	GRCGYC	2 cut(s)	168, 705
Hin1II	CATG	3 cut(s)	500, 623, 637
Hin6I	GCGC	2 cut(s)	180, 705
HinP1I	GCGC	2 cut(s)	180, 705
HinfI	GANTC	4 cut(s)	11, 150, 158, 718
HpaII	CCGG	3 cut(s)	127, 288, 327
HphI	GGTGA	3 cut(s)	373, 619, 658
Hpy166II	GTNNAC	1 cut(s)	592
Hpy188I	TCNGA	5 cut(s)	10, 306, 442, 642, 717
Hpy188III	TCNNGA	2 cut(s)	172, 581
Hpy8I	GTNNAC	1 cut(s)	592
HpyAV	CCTTC	7 cut(s)	49, 81, 259, 346, 637, 695, 718
HpyCH4III	ACNGT	2 cut(s)	116, 142
HpyCH4V	TGCA	4 cut(s)	298, 511, 623, 688
HpyF10VI	GCNNNNNNNGC	7 cut(s)	206, 323, 463, 487, 502, 527, 574
HpyF3I	CTNAG	1 cut(s)	603
Hsp92I	GRCGYC	2 cut(s)	168, 705
Hsp92II	CATG	3 cut(s)	500, 623, 637
HspAI	GCGC	2 cut(s)	180, 705
KasI	GGCGCC	1 cut(s)	704
KroI	GCCGGC	1 cut(s)	326
KroNI	GCCGGC	1 cut(s)	328
KspI	CCGCGG	1 cut(s)	166
Kzo9I	GATC	3 cut(s)	309, 318, 442
LguI	GCTCTTC	2 cut(s)	381, 609
LmnI	GCTCC	1 cut(s)	40
Lsp1109I	GCAGC	1 cut(s)	92
LweI	GCATC	2 cut(s)	475, 505
MaeI	CTAG	1 cut(s)	581
MaeIII	GTNAC	1 cut(s)	430
MalI	GATC	3 cut(s)	311, 320, 444
MboI	GATC	3 cut(s)	309, 318, 442
MboII	GAAGA	7 cut(s)	34, 203, 346, 398, 429, 626, 677
MhlI	GDGCHC	1 cut(s)	222
MluCI	AATT	4 cut(s)	30, 99, 248, 556
Mly113I	GGCGCC	1 cut(s)	705
MroNI	GCCGGC	1 cut(s)	326
MroXI	GAANNNNTTC	1 cut(s)	588
MseI	TTAA	4 cut(s)	29, 534, 630, 675
MspA1I	CMGCKG	1 cut(s)	165
MspI	CCGG	3 cut(s)	127, 288, 327
MspR9I	CCNGG	1 cut(s)	474
Mva1269I	GAATGC	1 cut(s)	241
MvaI	CCWGG	1 cut(s)	474
MvnI	CGCG	1 cut(s)	165
MwoI	GCNNNNNNNGC	7 cut(s)	206, 323, 463, 487, 502, 527, 574
NaeI	GCCGGC	1 cut(s)	328
NarI	GGCGCC	1 cut(s)	705
NdeI	CATATG	1 cut(s)	723
NdeII	GATC	3 cut(s)	309, 318, 442
NgoMIV	GCCGGC	1 cut(s)	326
NlaIII	CATG	3 cut(s)	500, 623, 637
NlaIV	GGNNCC	2 cut(s)	451, 706
NspI	RCATGY	2 cut(s)	623, 637
PaeI	GCATGC	1 cut(s)	623
PaqCI	CACCTGC	2 cut(s)	268, 496
PciSI	GCTCTTC	2 cut(s)	381, 609
PcsI	WCGNNNNNNNCGW	2 cut(s)	314, 318
PctI	GAATGC	1 cut(s)	241
PdiI	GCCGGC	1 cut(s)	328
PdmI	GAANNNNTTC	1 cut(s)	588
PfeI	GAWTC	4 cut(s)	11, 150, 158, 718
PflFI	GACNNNGTC	1 cut(s)	140
PkrI	GCNGC	6 cut(s)	107, 110, 167, 492, 495, 520
Ple19I	CGATCG	2 cut(s)	312, 321
PluTI	GGCGCC	1 cut(s)	708
PshBI	ATTAAT	1 cut(s)	29
Psp6I	CCWGG	1 cut(s)	472
PspGI	CCWGG	1 cut(s)	472
PspN4I	GGNNCC	2 cut(s)	451, 706
PsyI	GACNNNGTC	1 cut(s)	140
PvuI	CGATCG	2 cut(s)	312, 321
RsaI	GTAC	1 cut(s)	344
RsaNI	GTAC	1 cut(s)	343
SacII	CCGCGG	1 cut(s)	166
SapI	GCTCTTC	2 cut(s)	381, 609
SaqAI	TTAA	4 cut(s)	29, 534, 630, 675
SatI	GCNGC	6 cut(s)	106, 109, 166, 491, 494, 519
Sau3AI	GATC	3 cut(s)	309, 318, 442
ScrFI	CCNGG	1 cut(s)	474
SduI	GDGCHC	1 cut(s)	222
SfaNI	GCATC	2 cut(s)	475, 505
SfoI	GGCGCC	1 cut(s)	706
Sfr303I	CCGCGG	1 cut(s)	166
SgrBI	CCGCGG	1 cut(s)	166
SphI	GCATGC	1 cut(s)	623
Sse9I	AATT	4 cut(s)	30, 99, 248, 556
SsiI	CCGC	9 cut(s)	81, 108, 147, 163, 165, 491, 494, 503, 518
SspDI	GGCGCC	1 cut(s)	704
SspMI	CTAG	1 cut(s)	581
StyD4I	CCNGG	1 cut(s)	472
StyI	CCWWGG	2 cut(s)	368, 597
TaaI	ACNGT	2 cut(s)	116, 142
TaqI	TCGA	3 cut(s)	246, 312, 321
TasI	AATT	4 cut(s)	30, 99, 248, 556
TauI	GCSGC	5 cut(s)	111, 168, 493, 496, 521
TfiI	GAWTC	4 cut(s)	11, 150, 158, 718
Tru1I	TTAA	4 cut(s)	29, 534, 630, 675
Tru9I	TTAA	4 cut(s)	29, 534, 630, 675
TseI	GCWGC	1 cut(s)	105
TspDTI	ATGAA	1 cut(s)	17
TspGWI	ACGGA	1 cut(s)	418
Tth111I	GACNNNGTC	1 cut(s)	140
VspI	ATTAAT	1 cut(s)	29
XapI	RAATTY	1 cut(s)	248
XbaI	TCTAGA	1 cut(s)	580
XceI	RCATGY	2 cut(s)	623, 637
XmnI	GAANNNNTTC	1 cut(s)	588
XspI	CTAG	1 cut(s)	581

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.