Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0010323

Physical Location & Seq

Genomic Coordinates Reverse (-)

32929385 .. 32931191

Gene Structure 1807 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1275000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 735 bp

ATGAAATCGGAATATTTTTCTCTATTAATTGCTTCCTTCTTCGTCCTCCTCCTCCCCGCATTCTCATCCGCCGCCGAAGAATCATCAACCCCAATCGGCTGCGGCCACACCTCCCACCGCAACATGGCCACACTCGGCGGAATCCACGAGTCCCACGGCGCCCAAAACAGCGCCGAAGTTGAAGACCTCGCTCGCTTCGCAGTCCAAGAACACAACAAGAAGGAGAATGCTCTGCTCGAATTTGTGAGGGTTGTGAAGGCGAAAGAGCAGGTGGTTGCCGGAACTCTGCACCATCTGACGATCGAGGCGATCGAGGCCGGCAAGAAGAAGCTGTACCAAGCAAAGGTGTGGGTGAAGCCTTGGATGGGCTTCAAGGAAGTGCAGGAGTTCAAGCACGCTGATGAGGAGGAAACGCCGTCGGTTACCTCTTCCGATCTTGGCGTCAAGCAAGGCGGTCATCCTCCTGGGTGGCAATCTGTGCCGCCGCATGACCCGCAGGTGCAGGATGCGGCTAACCATGCTGTTAAGTCCCTCCAGCAGAGGTCTAATTCCTTGTTGCCTTATGAGCTTCAAGAAGTTGTTCACGCTCAGGCTGAGGTGGCAGAAGAGCATGCAAAGTTTAACATGCTTCTGAAGGTGAAGAGGGGAAGCAAAGAAGAGAAGTTTAAAGCCGAAGTGCATAAGAATATGGAAGGCACCTTCAGTCTGAATCAGATGGAGGCAGATCACTCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002020 GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005829 GO:0006950 GO:0006970 GO:0006972 GO:0006979 GO:0008150 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009892 GO:0010035 GO:0010466 GO:0010605 GO:0010951 GO:0012505 GO:0019222 GO:0019899 GO:0030162 GO:0030234 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0042221 GO:0043086 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044424 GO:0044444 GO:0044464 GO:0045861 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0050897 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0080090 GO:0098772 GO:1901700 GO:2000116 GO:2000117

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cystatin SQAPI

Protein Analysis

245

Amino Acids

27.11

Weight (kDa)

6.1

Isoelectric Point (pI)

42.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cystatin	PF00031	46 - 132	1.1e-17	Cystatin domain
SQAPI	PF16845	58 - 132	6.3e-20	Aspartic acid proteinase inhibitor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010323)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12490 AT3G12490
fragaria_vesca	FvH4_7g04140
malus_domestica	MD02G1275000.v1.1 MD07G1043000.v1.1
prunus_persica	Prupe.2G046600_v2.0.a1 Prupe.2G047100_v2.0.a1
pyrus_communis	pycom02g23510
rosa_chinensis	RchiOBHm_Chr1g0328231
rosa_laevigata	RLG00000019109 RLG00000029935
rosa_multiflora	Rmu_sc0004646.1_g000040
rosa_roxburghii	Rroxscaffold_4G00321700
rosa_rugosa	Rorug01G0074400 Rorug01G0074500
rosa_samantha	Rh1AG091700 Rh1BG074000 Rh1CG088100 Rh1DG095100
rosa_wichuraiana	Rw0G011030 Rw1G007080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	259, 487
Acc36I	ACCTGC	2 cut(s)	259, 487
AccB1I	GGYRCC	2 cut(s)	158, 695
AcoI	YGGCCR	2 cut(s)	103, 126
AcsI	RAATTY	1 cut(s)	239
AcuI	CTGAAG	2 cut(s)	653, 685
AcyI	GRCGYC	2 cut(s)	159, 441
AfaI	GTAC	1 cut(s)	335
AfiI	CCNNNNNNNGG	3 cut(s)	124, 343, 365
AgsI	TTSAA	4 cut(s)	182, 373, 391, 572
AjnI	CCWGG	1 cut(s)	463
AluBI	AGCT	2 cut(s)	331, 568
AluI	AGCT	2 cut(s)	331, 568
AoxI	GGCC	3 cut(s)	103, 126, 315
ApeKI	GCWGC	1 cut(s)	99
ApoI	RAATTY	1 cut(s)	239
AseI	ATTAAT	1 cut(s)	26
Asp700I	GAANNNNTTC	1 cut(s)	579
AspLEI	GCGC	2 cut(s)	161, 173
AsuHPI	GGTGA	2 cut(s)	364, 649
BalI	TGGCCA	1 cut(s)	128
BanI	GGYRCC	2 cut(s)	158, 695
BarI	GAAGNNNNNNTAC	2 cut(s)	317, 349
BauI	CACGAG	1 cut(s)	146
BbsI	GAAGAC	1 cut(s)	189
BbvCI	CCTCAGC	1 cut(s)	594
BbvI	GCAGC	1 cut(s)	86
BccI	CCATC	3 cut(s)	300, 358, 709
BceAI	ACGGC	2 cut(s)	172, 400
BciT130I	CCWGG	1 cut(s)	465
BfoI	RGCGCY	2 cut(s)	162, 174
BfuAI	ACCTGC	2 cut(s)	259, 487
BisI	GCNGC	6 cut(s)	72, 100, 103, 482, 485, 510
BlsI	GCNGC	6 cut(s)	73, 101, 104, 483, 486, 511
Bme1390I	CCNGG	1 cut(s)	465
BmiI	GGNNCC	2 cut(s)	160, 697
BmrFI	CCNGG	1 cut(s)	465
BmsI	GCATC	1 cut(s)	496
BpiI	GAAGAC	1 cut(s)	189
BpmI	CTGGAG	1 cut(s)	518
Bpu10I	CCTNAGC	2 cut(s)	588, 594
BsaHI	GRCGYC	2 cut(s)	159, 441
BsaJI	CCNNGG	3 cut(s)	154, 359, 464
Bsc4I	CCNNNNNNNGG	3 cut(s)	124, 343, 365
Bse118I	RCCGGY	1 cut(s)	317
BseBI	CCWGG	1 cut(s)	465
BseDI	CCNNGG	3 cut(s)	154, 359, 464
BseGI	GGATG	4 cut(s)	65, 369, 457, 511
BseLI	CCNNNNNNNGG	3 cut(s)	124, 343, 365
BseMII	CTCAG	2 cut(s)	585, 602
BseRI	GAGGAG	3 cut(s)	38, 41, 419
BseXI	GCAGC	1 cut(s)	86
BsgI	GTGCAG	3 cut(s)	272, 401, 521
Bsh1285I	CGRYCG	2 cut(s)	303, 312
BshFI	GGCC	3 cut(s)	105, 128, 317
BshNI	GGYRCC	2 cut(s)	158, 695
BsiEI	CGRYCG	2 cut(s)	303, 312
BsiSI	CCGG	2 cut(s)	279, 318
BslFI	GGGAC	2 cut(s)	136, 514
BslI	CCNNNNNNNGG	3 cut(s)	124, 343, 365
BsmFI	GGGAC	2 cut(s)	136, 514
BsmI	GAATGC	2 cut(s)	59, 232
BsnI	GGCC	3 cut(s)	105, 128, 317
Bsp143I	GATC	4 cut(s)	300, 309, 433, 724
BspANI	GGCC	3 cut(s)	105, 128, 317
BspCNI	CTCAG	2 cut(s)	586, 601
BspLI	GGNNCC	2 cut(s)	160, 697
BspMI	ACCTGC	2 cut(s)	259, 487
BspQI	GCTCTTC	1 cut(s)	600
BspT107I	GGYRCC	2 cut(s)	158, 695
BsrFI	RCCGGY	1 cut(s)	317
BssAI	RCCGGY	1 cut(s)	317
BssECI	CCNNGG	3 cut(s)	154, 359, 464
BssMI	GATC	4 cut(s)	300, 309, 433, 724
BssNI	GRCGYC	2 cut(s)	159, 441
BssSI	CACGAG	1 cut(s)	146
BssT1I	CCWWGG	1 cut(s)	359
Bst2BI	CACGAG	1 cut(s)	146
Bst2UI	CCWGG	1 cut(s)	465
Bst6I	CTCTTC	4 cut(s)	433, 600, 635, 651
BstACI	GRCGYC	2 cut(s)	159, 441
BstAPI	GCANNNNNTGC	1 cut(s)	478
BstC8I	GCNNGC	4 cut(s)	193, 319, 396, 612
BstDEI	CTNAG	2 cut(s)	588, 594
BstDSI	CCRYGG	1 cut(s)	154
BstEII	GGTNACC	1 cut(s)	421
BstF5I	GGATG	4 cut(s)	65, 369, 457, 511
BstH2I	RGCGCY	2 cut(s)	162, 174
BstHHI	GCGC	2 cut(s)	161, 173
BstKTI	GATC	4 cut(s)	303, 312, 436, 727
BstMBI	GATC	4 cut(s)	300, 309, 433, 724
BstMCI	CGRYCG	2 cut(s)	303, 312
BstMWI	GCNNNNNNNGC	7 cut(s)	197, 314, 478, 493, 518, 565, 599
BstNI	CCWGG	1 cut(s)	465
BstNSI	RCATGY	2 cut(s)	614, 628
BstPI	GGTNACC	1 cut(s)	421
BstSCI	CCNGG	1 cut(s)	463
BstV1I	GCAGC	1 cut(s)	86
BstV2I	GAAGAC	1 cut(s)	189
BsuRI	GGCC	3 cut(s)	105, 128, 317
BtgI	CCRYGG	1 cut(s)	154
BtsCI	GGATG	4 cut(s)	65, 369, 457, 511
BveI	ACCTGC	2 cut(s)	259, 487
Cac8I	GCNNGC	4 cut(s)	193, 319, 396, 612
CfoI	GCGC	2 cut(s)	161, 173
Cfr10I	RCCGGY	1 cut(s)	317
CseI	GACGC	1 cut(s)	430
Csp6I	GTAC	1 cut(s)	334
CviAII	CATG	5 cut(s)	124, 488, 518, 611, 625
CviQI	GTAC	1 cut(s)	334
DdeI	CTNAG	2 cut(s)	588, 594
DinI	GGCGCC	1 cut(s)	160
DpnI	GATC	4 cut(s)	302, 311, 435, 726
DpnII	GATC	4 cut(s)	300, 309, 433, 724
DraI	TTTAAA	1 cut(s)	667
EaeI	YGGCCR	2 cut(s)	103, 126
Eam1104I	CTCTTC	4 cut(s)	433, 600, 635, 651
EarI	CTCTTC	4 cut(s)	433, 600, 635, 651
EciI	GGCGGA	2 cut(s)	58, 153
Eco130I	CCWWGG	1 cut(s)	359
Eco57I	CTGAAG	2 cut(s)	653, 685
Eco91I	GGTNACC	1 cut(s)	421
EcoO65I	GGTNACC	1 cut(s)	421
EcoRII	CCWGG	1 cut(s)	463
EcoT14I	CCWWGG	1 cut(s)	359
EgeI	GGCGCC	1 cut(s)	160
EheI	GGCGCC	1 cut(s)	160
ErhI	CCWWGG	1 cut(s)	359
FaeI	CATG	5 cut(s)	127, 491, 521, 614, 628
FaiI	YATR	8 cut(s)	125, 489, 519, 564, 612, 626, 681, 689
FaqI	GGGAC	2 cut(s)	136, 514
FatI	CATG	5 cut(s)	123, 487, 517, 610, 624
FauI	CCCGC	2 cut(s)	64, 501
Fnu4HI	GCNGC	6 cut(s)	72, 100, 103, 482, 485, 510
FokI	GGATG	4 cut(s)	52, 376, 444, 518
Fsp4HI	GCNGC	6 cut(s)	72, 100, 103, 482, 485, 510
GlaI	GCGC	2 cut(s)	160, 172
GluI	GCNGC	6 cut(s)	72, 100, 103, 482, 485, 510
GsuI	CTGGAG	1 cut(s)	518
HaeII	RGCGCY	2 cut(s)	162, 174
HaeIII	GGCC	3 cut(s)	105, 128, 317
HapII	CCGG	2 cut(s)	279, 318
HgaI	GACGC	1 cut(s)	430
HhaI	GCGC	2 cut(s)	161, 173
Hin1I	GRCGYC	2 cut(s)	159, 441
Hin1II	CATG	5 cut(s)	127, 491, 521, 614, 628
Hin6I	GCGC	2 cut(s)	159, 171
HinP1I	GCGC	2 cut(s)	159, 171
HinfI	GANTC	4 cut(s)	80, 141, 149, 709
HpaII	CCGG	2 cut(s)	279, 318
HphI	GGTGA	2 cut(s)	364, 649
Hpy166II	GTNNAC	1 cut(s)	583
Hpy188I	TCNGA	6 cut(s)	10, 297, 433, 633, 708, 714
Hpy188III	TCNNGA	1 cut(s)	572
Hpy8I	GTNNAC	1 cut(s)	583
Hpy99I	CGWCG	1 cut(s)	421
HpyAV	CCTTC	6 cut(s)	46, 214, 250, 628, 686, 709
HpyCH4V	TGCA	5 cut(s)	289, 382, 502, 614, 679
HpyF10VI	GCNNNNNNNGC	7 cut(s)	197, 314, 478, 493, 518, 565, 599
HpyF3I	CTNAG	2 cut(s)	588, 594
Hsp92I	GRCGYC	2 cut(s)	159, 441
Hsp92II	CATG	5 cut(s)	127, 491, 521, 614, 628
HspAI	GCGC	2 cut(s)	159, 171
KasI	GGCGCC	1 cut(s)	158
KroI	GCCGGC	1 cut(s)	317
KroNI	GCCGGC	1 cut(s)	319
Kzo9I	GATC	4 cut(s)	300, 309, 433, 724
LguI	GCTCTTC	1 cut(s)	600
Lsp1109I	GCAGC	1 cut(s)	86
LweI	GCATC	1 cut(s)	496
MaeIII	GTNAC	1 cut(s)	421
MalI	GATC	4 cut(s)	302, 311, 435, 726
MboI	GATC	4 cut(s)	300, 309, 433, 724
MboII	GAAGA	8 cut(s)	31, 89, 194, 337, 420, 617, 652, 668
MlsI	TGGCCA	1 cut(s)	128
MluCI	AATT	3 cut(s)	27, 239, 547
MluNI	TGGCCA	1 cut(s)	128
Mly113I	GGCGCC	1 cut(s)	159
MlyI	GAGTC	1 cut(s)	158
Mox20I	TGGCCA	1 cut(s)	128
MroNI	GCCGGC	1 cut(s)	317
MroXI	GAANNNNTTC	1 cut(s)	579
MscI	TGGCCA	1 cut(s)	128
MseI	TTAA	4 cut(s)	26, 525, 621, 666
MslI	CAYNNNNRTG	1 cut(s)	399
Msp20I	TGGCCA	1 cut(s)	128
MspI	CCGG	2 cut(s)	279, 318
MspR9I	CCNGG	1 cut(s)	465
Mva1269I	GAATGC	2 cut(s)	59, 232
MvaI	CCWGG	1 cut(s)	465
MwoI	GCNNNNNNNGC	7 cut(s)	197, 314, 478, 493, 518, 565, 599
NaeI	GCCGGC	1 cut(s)	319
NarI	GGCGCC	1 cut(s)	159
NdeII	GATC	4 cut(s)	300, 309, 433, 724
NgoMIV	GCCGGC	1 cut(s)	317
NlaIII	CATG	5 cut(s)	127, 491, 521, 614, 628
NlaIV	GGNNCC	2 cut(s)	160, 697
NmeAIII	GCCGAG	1 cut(s)	114
NspI	RCATGY	2 cut(s)	614, 628
PaeI	GCATGC	1 cut(s)	614
PaqCI	CACCTGC	2 cut(s)	259, 487
PciSI	GCTCTTC	1 cut(s)	600
PcsI	WCGNNNNNNNCGW	2 cut(s)	305, 309
PctI	GAATGC	2 cut(s)	59, 232
PdiI	GCCGGC	1 cut(s)	319
PdmI	GAANNNNTTC	1 cut(s)	579
PfeI	GAWTC	3 cut(s)	80, 141, 709
PkrI	GCNGC	6 cut(s)	73, 101, 104, 483, 486, 511
Ple19I	CGATCG	2 cut(s)	303, 312
PleI	GAGTC	1 cut(s)	157
PluTI	GGCGCC	1 cut(s)	162
PpsI	GAGTC	1 cut(s)	157
PshBI	ATTAAT	1 cut(s)	26
Psp6I	CCWGG	1 cut(s)	463
PspEI	GGTNACC	1 cut(s)	421
PspGI	CCWGG	1 cut(s)	463
PspN4I	GGNNCC	2 cut(s)	160, 697
PvuI	CGATCG	2 cut(s)	303, 312
RsaI	GTAC	1 cut(s)	335
RsaNI	GTAC	1 cut(s)	334
RseI	CAYNNNNRTG	1 cut(s)	399
SapI	GCTCTTC	1 cut(s)	600
SaqAI	TTAA	4 cut(s)	26, 525, 621, 666
SatI	GCNGC	6 cut(s)	72, 100, 103, 482, 485, 510
Sau3AI	GATC	4 cut(s)	300, 309, 433, 724
SchI	GAGTC	1 cut(s)	158
ScrFI	CCNGG	1 cut(s)	465
SfaNI	GCATC	1 cut(s)	496
SfoI	GGCGCC	1 cut(s)	160
SmiMI	CAYNNNNRTG	1 cut(s)	399
SphI	GCATGC	1 cut(s)	614
Sse9I	AATT	3 cut(s)	27, 239, 547
SspDI	GGCGCC	1 cut(s)	158
SspI	AATATT	1 cut(s)	14
StyD4I	CCNGG	1 cut(s)	463
StyI	CCWWGG	1 cut(s)	359
TaqI	TCGA	3 cut(s)	237, 303, 312
TasI	AATT	3 cut(s)	27, 239, 547
TauI	GCSGC	5 cut(s)	74, 105, 484, 487, 512
TfiI	GAWTC	3 cut(s)	80, 141, 709
Tru1I	TTAA	4 cut(s)	26, 525, 621, 666
Tru9I	TTAA	4 cut(s)	26, 525, 621, 666
TseI	GCWGC	1 cut(s)	99
TspDTI	ATGAA	1 cut(s)	17
VspI	ATTAAT	1 cut(s)	26
XapI	RAATTY	1 cut(s)	239
XceI	RCATGY	2 cut(s)	614, 628
XmnI	GAANNNNTTC	1 cut(s)	579

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.