Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0010323

Physical Location & Seq

Genomic Coordinates Reverse (-)

51312609 .. 51314381

Gene Structure 1773 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00321700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 726 bp

ATGAAATCGAAACCACAATCGAACTCATCGACTTCCTCATTCTTCTCTCTCGTCCTCTTCTTCCTCTTCTCCTTCATCGCACTCTCTTCATCCTCTACAATGGCCACCCTCGGCGGAATTCGCGACTCCCCTGCCGGCTCCGAGAACAGCCTCGAGACCGAATCCCTCGCCCGCTTCGCCGTCGACGAGCATAACAAGAAGGAGAATGCTCTGCTGGAGTTTGTTCGAGTGGTGAAGGCTAAGGAACAGGTGGTTGCCGGCACTATGCACCATCTGGTTGTGGAGGCCATCGACGGCGGCAAGAAGAAGCTTTATGAAGCCAAGGTCTGGGTCAAGCCTTGGTTGGGATTTAAAGAGGTTCAGGAATTCAAGCACGCTGAGGATCCCGAAGCTGCATCGGAAACGCCGTCGTTTACTGCTTCCGATTTGGGCGTTAAGCAAGGTGGGCATGCTCCAGGGTGGCAAGATGTGCATCCTCATGATCCTCAAGTTCAAGATGCAGCCAACCATGCTGTGAAGAGCCTTCAGCAAAGGTCCAATTCATTGTTCCCTTATGAGCTTCAAGAGGTTGTCCATGCTAAGGCAGAGGTGATAGAAGATCATGCCAAATTCAACATGCTTCTTAAGCTGAAGAGGGGAGACAAAGAAGAGAAATATAGAGTTGAAGTGCACAAGAACAATGAAGGTGCCTACAATCTAAATCAGATGGAGGCGCAGCACTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002020 GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005829 GO:0006950 GO:0006970 GO:0006972 GO:0006979 GO:0008150 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009892 GO:0010035 GO:0010466 GO:0010605 GO:0010951 GO:0012505 GO:0019222 GO:0019899 GO:0030162 GO:0030234 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0042221 GO:0043086 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044424 GO:0044444 GO:0044464 GO:0045861 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0050897 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0080090 GO:0098772 GO:1901700 GO:2000116 GO:2000117

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cystatin SQAPI

Protein Analysis

241

Amino Acids

26.87

Weight (kDa)

6.18

Isoelectric Point (pI)

41.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cystatin	PF00031	48 - 125	1.6e-16	Cystatin domain
SQAPI	PF16845	54 - 125	3.7e-20	Aspartic acid proteinase inhibitor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010323)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12490 AT3G12490
fragaria_vesca	FvH4_7g04140
malus_domestica	MD02G1275000.v1.1 MD07G1043000.v1.1
prunus_persica	Prupe.2G046600_v2.0.a1 Prupe.2G047100_v2.0.a1
pyrus_communis	pycom02g23510
rosa_chinensis	RchiOBHm_Chr1g0328231
rosa_laevigata	RLG00000019109 RLG00000029935
rosa_multiflora	Rmu_sc0004646.1_g000040
rosa_roxburghii	Rroxscaffold_4G00321700
rosa_rugosa	Rorug01G0074400 Rorug01G0074500
rosa_samantha	Rh1AG091700 Rh1BG074000 Rh1CG088100 Rh1DG095100
rosa_wichuraiana	Rw0G011030 Rw1G007080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	686
AccB7I	CCANNNNNTGG	1 cut(s)	327
AccI	GTMKAC	1 cut(s)	183
AccII	CGCG	1 cut(s)	123
AciI	CCGC	3 cut(s)	114, 172, 297
AclWI	GGATC	3 cut(s)	377, 390, 476
AcoI	YGGCCR	1 cut(s)	102
AcsI	RAATTY	3 cut(s)	117, 365, 608
AcuI	CTGAAG	2 cut(s)	509, 650
AfiI	CCNNNNNNNGG	3 cut(s)	327, 344, 580
AflII	CTTAAG	1 cut(s)	623
AgsI	TTSAA	5 cut(s)	370, 494, 563, 613, 665
AjnI	CCWGG	1 cut(s)	454
AjuI	GAANNNNNNNTTGG	2 cut(s)	530, 562
AluBI	AGCT	4 cut(s)	310, 392, 559, 628
AluI	AGCT	4 cut(s)	310, 392, 559, 628
Alw21I	GWGCWC	1 cut(s)	672
Alw26I	GTCTC	2 cut(s)	149, 633
Alw44I	GTGCAC	1 cut(s)	668
AlwI	GGATC	3 cut(s)	377, 390, 476
Ama87I	CYCGRG	1 cut(s)	152
AoxI	GGCC	2 cut(s)	102, 285
ApaLI	GTGCAC	1 cut(s)	668
ApeKI	GCWGC	3 cut(s)	392, 500, 715
ApoI	RAATTY	3 cut(s)	117, 365, 608
AspLEI	GCGC	1 cut(s)	715
AspS9I	GGNCC	1 cut(s)	534
AsuHPI	GGTGA	2 cut(s)	244, 601
AvaI	CYCGRG	1 cut(s)	152
AvaII	GGWCC	1 cut(s)	534
BaeGI	GKGCMC	1 cut(s)	672
BalI	TGGCCA	1 cut(s)	104
BamHI	GGATCC	1 cut(s)	382
BanI	GGYRCC	1 cut(s)	686
Bbv12I	GWGCWC	1 cut(s)	672
BbvCI	CCTCAGC	1 cut(s)	378
BbvI	GCAGC	2 cut(s)	379, 512
BccI	CCATC	3 cut(s)	279, 296, 700
BceAI	ACGGC	3 cut(s)	164, 310, 391
BcgI	CGANNNNNNTGC	2 cut(s)	113, 147
BciT130I	CCWGG	1 cut(s)	456
BcoDI	GTCTC	2 cut(s)	149, 633
BfrI	CTTAAG	1 cut(s)	623
BisI	GCNGC	4 cut(s)	298, 393, 501, 716
BlsI	GCNGC	4 cut(s)	299, 394, 502, 717
Bme1390I	CCNGG	1 cut(s)	456
Bme18I	GGWCC	1 cut(s)	534
BmeT110I	CYCGRG	1 cut(s)	152
BmgT120I	GGNCC	1 cut(s)	534
BmiI	GGNNCC	3 cut(s)	139, 384, 688
BmrFI	CCNGG	1 cut(s)	456
BmsI	GCATC	3 cut(s)	404, 481, 487
BpmI	CTGGAG	2 cut(s)	236, 438
Bpu10I	CCTNAGC	3 cut(s)	240, 378, 579
BpuEI	CTTGAG	1 cut(s)	471
BsaBI	GATNNNNATC	1 cut(s)	471
BsaI	GGTCTC	1 cut(s)	149
BsaJI	CCNNGG	4 cut(s)	109, 321, 338, 455
Bsc4I	CCNNNNNNNGG	3 cut(s)	327, 344, 580
Bse118I	RCCGGY	2 cut(s)	134, 257
Bse8I	GATNNNNATC	1 cut(s)	471
BseBI	CCWGG	1 cut(s)	456
BseDI	CCNNGG	4 cut(s)	109, 321, 338, 455
BseGI	GGATG	2 cut(s)	89, 472
BseJI	GATNNNNATC	1 cut(s)	471
BseLI	CCNNNNNNNGG	3 cut(s)	327, 344, 580
BseMII	CTCAG	1 cut(s)	369
BseSI	GKGCMC	1 cut(s)	672
BseXI	GCAGC	2 cut(s)	379, 512
Bsh1236I	CGCG	1 cut(s)	123
BshFI	GGCC	2 cut(s)	104, 287
BshNI	GGYRCC	1 cut(s)	686
BsiHKAI	GWGCWC	1 cut(s)	672
BsiHKCI	CYCGRG	1 cut(s)	152
BsiSI	CCGG	2 cut(s)	135, 258
BslI	CCNNNNNNNGG	3 cut(s)	327, 344, 580
BsmAI	GTCTC	2 cut(s)	149, 633
BsmI	GAATGC	1 cut(s)	211
BsnI	GGCC	2 cut(s)	104, 287
Bso31I	GGTCTC	1 cut(s)	149
BsoBI	CYCGRG	1 cut(s)	152
Bsp1286I	GDGCHC	1 cut(s)	672
Bsp143I	GATC	3 cut(s)	382, 481, 598
Bsp68I	TCGCGA	1 cut(s)	123
BspACI	CCGC	3 cut(s)	114, 172, 297
BspANI	GGCC	2 cut(s)	104, 287
BspCNI	CTCAG	1 cut(s)	370
BspFNI	CGCG	1 cut(s)	123
BspHI	TCATGA	1 cut(s)	478
BspLI	GGNNCC	3 cut(s)	139, 384, 688
BspPI	GGATC	3 cut(s)	377, 390, 476
BspQI	GCTCTTC	1 cut(s)	512
BspT107I	GGYRCC	1 cut(s)	686
BspTI	CTTAAG	1 cut(s)	623
BspTNI	GGTCTC	1 cut(s)	149
BsrFI	RCCGGY	2 cut(s)	134, 257
BssAI	RCCGGY	2 cut(s)	134, 257
BssECI	CCNNGG	4 cut(s)	109, 321, 338, 455
BssMI	GATC	3 cut(s)	382, 481, 598
BssT1I	CCWWGG	2 cut(s)	321, 338
Bst2UI	CCWGG	1 cut(s)	456
Bst6I	CTCTTC	6 cut(s)	62, 71, 91, 512, 626, 642
BstAFI	CTTAAG	1 cut(s)	623
BstAPI	GCANNNNNTGC	1 cut(s)	469
BstC8I	GCNNGC	5 cut(s)	136, 172, 259, 375, 450
BstDEI	CTNAG	3 cut(s)	240, 378, 579
BstF5I	GGATG	2 cut(s)	89, 472
BstFNI	CGCG	1 cut(s)	123
BstHHI	GCGC	1 cut(s)	715
BstKTI	GATC	3 cut(s)	385, 484, 601
BstMAI	GTCTC	2 cut(s)	149, 633
BstMBI	GATC	3 cut(s)	382, 481, 598
BstMWI	GCNNNNNNNGC	6 cut(s)	120, 176, 445, 469, 509, 625
BstNI	CCWGG	1 cut(s)	456
BstNSI	RCATGY	2 cut(s)	452, 619
BstSCI	CCNGG	1 cut(s)	454
BstSLI	GKGCMC	1 cut(s)	672
BstUI	CGCG	1 cut(s)	123
BstV1I	GCAGC	2 cut(s)	379, 512
BstX2I	RGATCY	1 cut(s)	382
BstYI	RGATCY	1 cut(s)	382
BsuRI	GGCC	2 cut(s)	104, 287
BtgZI	GCGATG	1 cut(s)	61
BtsCI	GGATG	2 cut(s)	89, 472
BtuMI	TCGCGA	1 cut(s)	123
Cac8I	GCNNGC	5 cut(s)	136, 172, 259, 375, 450
CciI	TCATGA	1 cut(s)	478
CfoI	GCGC	1 cut(s)	715
Cfr10I	RCCGGY	2 cut(s)	134, 257
Cfr13I	GGNCC	1 cut(s)	534
CviAII	CATG	6 cut(s)	449, 479, 509, 575, 602, 616
DdeI	CTNAG	3 cut(s)	240, 378, 579
DpnI	GATC	3 cut(s)	384, 483, 600
DpnII	GATC	3 cut(s)	382, 481, 598
DraI	TTTAAA	1 cut(s)	352
EaeI	YGGCCR	1 cut(s)	102
Eam1104I	CTCTTC	6 cut(s)	62, 71, 91, 512, 626, 642
EarI	CTCTTC	6 cut(s)	62, 71, 91, 512, 626, 642
EciI	GGCGGA	1 cut(s)	129
Eco130I	CCWWGG	2 cut(s)	321, 338
Eco31I	GGTCTC	1 cut(s)	149
Eco47I	GGWCC	1 cut(s)	534
Eco57I	CTGAAG	2 cut(s)	509, 650
Eco88I	CYCGRG	1 cut(s)	152
EcoRI	GAATTC	2 cut(s)	117, 365
EcoRII	CCWGG	1 cut(s)	454
EcoT14I	CCWWGG	2 cut(s)	321, 338
ErhI	CCWWGG	2 cut(s)	321, 338
FaeI	CATG	6 cut(s)	452, 482, 512, 578, 605, 619
FatI	CATG	6 cut(s)	448, 478, 508, 574, 601, 615
FauI	CCCGC	1 cut(s)	179
FblI	GTMKAC	1 cut(s)	183
Fnu4HI	GCNGC	4 cut(s)	298, 393, 501, 716
FokI	GGATG	2 cut(s)	76, 459
Fsp4HI	GCNGC	4 cut(s)	298, 393, 501, 716
GlaI	GCGC	1 cut(s)	714
GluI	GCNGC	4 cut(s)	298, 393, 501, 716
GsuI	CTGGAG	2 cut(s)	236, 438
HaeIII	GGCC	2 cut(s)	104, 287
HapII	CCGG	2 cut(s)	135, 258
HhaI	GCGC	1 cut(s)	715
Hin1II	CATG	6 cut(s)	452, 482, 512, 578, 605, 619
Hin6I	GCGC	1 cut(s)	713
HinP1I	GCGC	1 cut(s)	713
HincII	GTYRAC	1 cut(s)	184
HindII	GTYRAC	1 cut(s)	184
HindIII	AAGCTT	1 cut(s)	308
HinfI	GANTC	2 cut(s)	125, 161
HpaII	CCGG	2 cut(s)	135, 258
HphI	GGTGA	2 cut(s)	244, 601
Hpy166II	GTNNAC	3 cut(s)	184, 414, 670
Hpy188I	TCNGA	4 cut(s)	142, 400, 424, 705
Hpy188III	TCNNGA	7 cut(s)	122, 154, 362, 386, 479, 494, 563
Hpy8I	GTNNAC	3 cut(s)	184, 414, 670
Hpy99I	CGWCG	4 cut(s)	185, 188, 296, 412
HpyAV	CCTTC	5 cut(s)	82, 193, 229, 533, 677
HpyCH4V	TGCA	5 cut(s)	268, 395, 472, 500, 670
HpyF10VI	GCNNNNNNNGC	6 cut(s)	120, 176, 445, 469, 509, 625
HpyF3I	CTNAG	3 cut(s)	240, 378, 579
Hsp92II	CATG	6 cut(s)	452, 482, 512, 578, 605, 619
HspAI	GCGC	1 cut(s)	713
KroI	GCCGGC	2 cut(s)	134, 257
KroNI	GCCGGC	2 cut(s)	136, 259
Kzo9I	GATC	3 cut(s)	382, 481, 598
LguI	GCTCTTC	1 cut(s)	512
LmnI	GCTCC	2 cut(s)	143, 457
Lsp1109I	GCAGC	2 cut(s)	379, 512
LweI	GCATC	3 cut(s)	404, 481, 487
MalI	GATC	3 cut(s)	384, 483, 600
MboI	GATC	3 cut(s)	382, 481, 598
MflI	RGATCY	1 cut(s)	382
MhlI	GDGCHC	1 cut(s)	672
MlsI	TGGCCA	1 cut(s)	104
MluCI	AATT	4 cut(s)	117, 365, 538, 608
MluNI	TGGCCA	1 cut(s)	104
MlyI	GAGTC	1 cut(s)	119
Mox20I	TGGCCA	1 cut(s)	104
MroNI	GCCGGC	2 cut(s)	134, 257
MscI	TGGCCA	1 cut(s)	104
MseI	TTAA	4 cut(s)	351, 435, 624, 724
MslI	CAYNNNNRTG	1 cut(s)	477
Msp20I	TGGCCA	1 cut(s)	104
MspCI	CTTAAG	1 cut(s)	623
MspI	CCGG	2 cut(s)	135, 258
MspR9I	CCNGG	1 cut(s)	456
Mva1269I	GAATGC	1 cut(s)	211
MvaI	CCWGG	1 cut(s)	456
MvnI	CGCG	1 cut(s)	123
MwoI	GCNNNNNNNGC	6 cut(s)	120, 176, 445, 469, 509, 625
NaeI	GCCGGC	2 cut(s)	136, 259
NdeII	GATC	3 cut(s)	382, 481, 598
NgoMIV	GCCGGC	2 cut(s)	134, 257
NlaIII	CATG	6 cut(s)	452, 482, 512, 578, 605, 619
NlaIV	GGNNCC	3 cut(s)	139, 384, 688
NmeAIII	GCCGAG	1 cut(s)	90
NruI	TCGCGA	1 cut(s)	123
NspI	RCATGY	2 cut(s)	452, 619
PaeI	GCATGC	1 cut(s)	452
PaeR7I	CTCGAG	1 cut(s)	152
PagI	TCATGA	1 cut(s)	478
PciSI	GCTCTTC	1 cut(s)	512
PcsI	WCGNNNNNNNCGW	3 cut(s)	26, 183, 404
PctI	GAATGC	1 cut(s)	211
PdiI	GCCGGC	2 cut(s)	136, 259
PfeI	GAWTC	1 cut(s)	161
PflMI	CCANNNNNTGG	1 cut(s)	327
PkrI	GCNGC	4 cut(s)	299, 394, 502, 717
PleI	GAGTC	1 cut(s)	119
PpsI	GAGTC	1 cut(s)	119
Psp6I	CCWGG	1 cut(s)	454
PspGI	CCWGG	1 cut(s)	454
PspN4I	GGNNCC	3 cut(s)	139, 384, 688
PspPI	GGNCC	1 cut(s)	534
PsuI	RGATCY	1 cut(s)	382
RruI	TCGCGA	1 cut(s)	123
RseI	CAYNNNNRTG	1 cut(s)	477
SalI	GTCGAC	1 cut(s)	182
SapI	GCTCTTC	1 cut(s)	512
SaqAI	TTAA	4 cut(s)	351, 435, 624, 724
SatI	GCNGC	4 cut(s)	298, 393, 501, 716
Sau3AI	GATC	3 cut(s)	382, 481, 598
Sau96I	GGNCC	1 cut(s)	534
SchI	GAGTC	1 cut(s)	119
ScrFI	CCNGG	1 cut(s)	456
SduI	GDGCHC	1 cut(s)	672
SfaNI	GCATC	3 cut(s)	404, 481, 487
Sfr274I	CTCGAG	1 cut(s)	152
SgrDI	CGTCGACG	1 cut(s)	182
SinI	GGWCC	1 cut(s)	534
SlaI	CTCGAG	1 cut(s)	152
SmiMI	CAYNNNNRTG	1 cut(s)	477
SmlI	CTYRAG	3 cut(s)	152, 486, 623
SmoI	CTYRAG	3 cut(s)	152, 486, 623
SphI	GCATGC	1 cut(s)	452
Sse9I	AATT	4 cut(s)	117, 365, 538, 608
SsiI	CCGC	3 cut(s)	114, 172, 297
StyD4I	CCNGG	1 cut(s)	454
StyI	CCWWGG	2 cut(s)	321, 338
TaqI	TCGA	7 cut(s)	8, 20, 29, 153, 183, 226, 291
TaqII	GACCGA	1 cut(s)	173
TasI	AATT	4 cut(s)	117, 365, 538, 608
TauI	GCSGC	1 cut(s)	300
TfiI	GAWTC	1 cut(s)	161
Tru1I	TTAA	4 cut(s)	351, 435, 624, 724
Tru9I	TTAA	4 cut(s)	351, 435, 624, 724
TseI	GCWGC	3 cut(s)	392, 500, 715
TspDTI	ATGAA	6 cut(s)	17, 64, 78, 330, 531, 696
Van91I	CCANNNNNTGG	1 cut(s)	327
Vha464I	CTTAAG	1 cut(s)	623
VneI	GTGCAC	1 cut(s)	668
VpaK11BI	GGWCC	1 cut(s)	534
XapI	RAATTY	3 cut(s)	117, 365, 608
XceI	RCATGY	2 cut(s)	452, 619
XhoI	CTCGAG	1 cut(s)	152
XmiI	GTMKAC	1 cut(s)	183

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.