Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0010323

Physical Location & Seq

Genomic Coordinates Forward (+)

4771703 .. 4773686

Gene Structure 1984 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g04140.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 708 bp

ATGAAATTGAGATCCCAATCGAACTCATCGCCTTCCTTCGTCTTCTTCTTCGTCCTCTTCTTCTCCTTCATCGCTCTCTCTGAAATGGCCACCCTCGGCGGAATCGGCGACTCCCCTGCCGGCTCCGAGAACAGCCTCGAAACCGAAGCCCTCGGTCGCTTCGCCGTCGACGACCACAACCAGAAGCAGAATGGTATGCTGGAGTTTGTTCGGGTGGTGAAGGCCAAGGAGCAGGTGGTTGCCGGCACTCTGCACCATCTGGTGGTGGAGGCCATCGACGGCGGCAAGAAGAAGCTCTATGAAGCCAAGGTCTGGGTCAAGCCTTGGTTGAACTTTAAACAAGTTCAGGAATTCAAGCACGCTGGTGAGCCCGAGACTGTGTCCGGAACGCCGTCGTTTACCTCTTCCGATTTGGGGGTTAAGCAAGGTGGGCATGCTCCAGGGTGGCAAGATGTGCATCCTCATGATCCTCAAGTTCAAGATGCAGCCAACCATGCTGTGAAGAGCCTTCAGCAGAAGTCCAATTCGTTGTTCCCTTATGAGCTTCAAGAGGTTGTCCATGCTAAGGCTGAGGTGATGGAAGAGCATGCCAAGTTCAACATGCTACTCAAACTGAAGAGGGGAGACAAAGAAGAGAAATATAAAGTTGAAGTGCACAAGAACAATGAAGGTGCCTACAATCTGAATCAGATGGAGGTGGAGCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002020 GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005829 GO:0006950 GO:0006970 GO:0006972 GO:0006979 GO:0008150 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009892 GO:0010035 GO:0010466 GO:0010605 GO:0010951 GO:0012505 GO:0019222 GO:0019899 GO:0030162 GO:0030234 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0042221 GO:0043086 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044424 GO:0044444 GO:0044464 GO:0045861 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0050897 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0080090 GO:0098772 GO:1901700 GO:2000116 GO:2000117

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cystatin SQAPI

Protein Analysis

236

Amino Acids

26.29

Weight (kDa)

6.18

Isoelectric Point (pI)

37.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cystatin	PF00031	43 - 120	5.7e-13	Cystatin domain
SQAPI	PF16845	49 - 120	1.3e-18	Aspartic acid proteinase inhibitor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010323)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12490 AT3G12490
fragaria_vesca	FvH4_7g04140
malus_domestica	MD02G1275000.v1.1 MD07G1043000.v1.1
prunus_persica	Prupe.2G046600_v2.0.a1 Prupe.2G047100_v2.0.a1
pyrus_communis	pycom02g23510
rosa_chinensis	RchiOBHm_Chr1g0328231
rosa_laevigata	RLG00000019109 RLG00000029935
rosa_multiflora	Rmu_sc0004646.1_g000040
rosa_roxburghii	Rroxscaffold_4G00321700
rosa_rugosa	Rorug01G0074400 Rorug01G0074500
rosa_samantha	Rh1AG091700 Rh1BG074000 Rh1CG088100 Rh1DG095100
rosa_wichuraiana	Rw0G011030 Rw1G007080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	223
Acc36I	ACCTGC	1 cut(s)	223
AccB1I	GGYRCC	1 cut(s)	671
AccB7I	CCANNNNNTGG	2 cut(s)	262, 312
AccI	GTMKAC	1 cut(s)	168
AccIII	TCCGGA	1 cut(s)	383
AciI	CCGC	2 cut(s)	99, 282
AclWI	GGATC	2 cut(s)	6, 461
AcoI	YGGCCR	1 cut(s)	87
AcsI	RAATTY	1 cut(s)	350
AcuI	CTGAAG	2 cut(s)	494, 635
AfiI	CCNNNNNNNGG	4 cut(s)	262, 312, 414, 565
AgsI	TTSAA	6 cut(s)	331, 355, 479, 548, 598, 650
AjnI	CCWGG	1 cut(s)	439
AjuI	GAANNNNNNNTTGG	2 cut(s)	515, 547
AleI	CACNNNNGTG	1 cut(s)	363
AluBI	AGCT	2 cut(s)	295, 544
AluI	AGCT	2 cut(s)	295, 544
Alw21I	GWGCWC	2 cut(s)	657, 705
Alw26I	GTCTC	2 cut(s)	368, 618
Alw44I	GTGCAC	1 cut(s)	653
AlwI	GGATC	2 cut(s)	6, 461
Ama87I	CYCGRG	1 cut(s)	371
Aor13HI	TCCGGA	1 cut(s)	383
AoxI	GGCC	3 cut(s)	87, 222, 270
ApaLI	GTGCAC	1 cut(s)	653
ApeKI	GCWGC	1 cut(s)	485
ApoI	RAATTY	1 cut(s)	350
AsuHPI	GGTGA	3 cut(s)	229, 377, 586
AvaI	CYCGRG	1 cut(s)	371
BaeGI	GKGCMC	1 cut(s)	657
BalI	TGGCCA	1 cut(s)	89
BanI	GGYRCC	1 cut(s)	671
BanII	GRGCYC	1 cut(s)	372
BbsI	GAAGAC	1 cut(s)	34
Bbv12I	GWGCWC	2 cut(s)	657, 705
BbvCI	CCTCAGC	1 cut(s)	570
BbvI	GCAGC	1 cut(s)	497
BccI	CCATC	4 cut(s)	264, 281, 571, 685
BceAI	ACGGC	3 cut(s)	149, 295, 376
BcgI	CGANNNNNNTGC	2 cut(s)	98, 132
BciT130I	CCWGG	1 cut(s)	441
BcoDI	GTCTC	2 cut(s)	368, 618
BfuAI	ACCTGC	1 cut(s)	223
BisI	GCNGC	2 cut(s)	283, 486
BlsI	GCNGC	2 cut(s)	284, 487
Bme1390I	CCNGG	1 cut(s)	441
BmeT110I	CYCGRG	1 cut(s)	371
BmiI	GGNNCC	2 cut(s)	124, 673
BmrFI	CCNGG	1 cut(s)	441
BmsI	GCATC	2 cut(s)	466, 472
BpiI	GAAGAC	1 cut(s)	34
BpmI	CTGGAG	2 cut(s)	221, 423
Bpu10I	CCTNAGC	2 cut(s)	564, 570
BpuEI	CTTGAG	1 cut(s)	456
BsaBI	GATNNNNATC	2 cut(s)	16, 456
BsaJI	CCNNGG	6 cut(s)	94, 151, 225, 306, 323, 440
BsaWI	WCCGGW	1 cut(s)	383
Bsc4I	CCNNNNNNNGG	4 cut(s)	262, 312, 414, 565
Bse118I	RCCGGY	2 cut(s)	119, 242
Bse8I	GATNNNNATC	2 cut(s)	16, 456
BseAI	TCCGGA	1 cut(s)	383
BseBI	CCWGG	1 cut(s)	441
BseDI	CCNNGG	6 cut(s)	94, 151, 225, 306, 323, 440
BseGI	GGATG	1 cut(s)	457
BseJI	GATNNNNATC	2 cut(s)	16, 456
BseLI	CCNNNNNNNGG	4 cut(s)	262, 312, 414, 565
BseMII	CTCAG	1 cut(s)	561
BseSI	GKGCMC	1 cut(s)	657
BseXI	GCAGC	1 cut(s)	497
BsgI	GTGCAG	1 cut(s)	236
Bsh1285I	CGRYCG	1 cut(s)	157
BshFI	GGCC	3 cut(s)	89, 224, 272
BshNI	GGYRCC	1 cut(s)	671
BsiEI	CGRYCG	1 cut(s)	157
BsiHKAI	GWGCWC	2 cut(s)	657, 705
BsiHKCI	CYCGRG	1 cut(s)	371
BsiSI	CCGG	3 cut(s)	120, 243, 384
BslI	CCNNNNNNNGG	4 cut(s)	262, 312, 414, 565
BsmAI	GTCTC	2 cut(s)	368, 618
BsnI	GGCC	3 cut(s)	89, 224, 272
BsoBI	CYCGRG	1 cut(s)	371
Bsp1286I	GDGCHC	3 cut(s)	372, 657, 705
Bsp13I	TCCGGA	1 cut(s)	383
Bsp143I	GATC	2 cut(s)	11, 466
BspACI	CCGC	2 cut(s)	99, 282
BspANI	GGCC	3 cut(s)	89, 224, 272
BspCNI	CTCAG	1 cut(s)	562
BspEI	TCCGGA	1 cut(s)	383
BspHI	TCATGA	1 cut(s)	463
BspLI	GGNNCC	2 cut(s)	124, 673
BspMI	ACCTGC	1 cut(s)	223
BspPI	GGATC	2 cut(s)	6, 461
BspQI	GCTCTTC	2 cut(s)	497, 576
BspT107I	GGYRCC	1 cut(s)	671
BsrFI	RCCGGY	2 cut(s)	119, 242
BssAI	RCCGGY	2 cut(s)	119, 242
BssECI	CCNNGG	6 cut(s)	94, 151, 225, 306, 323, 440
BssMI	GATC	2 cut(s)	11, 466
BssT1I	CCWWGG	3 cut(s)	225, 306, 323
Bst2UI	CCWGG	1 cut(s)	441
Bst4CI	ACNGT	1 cut(s)	379
Bst6I	CTCTTC	6 cut(s)	62, 409, 497, 576, 611, 627
BstAPI	GCANNNNNTGC	1 cut(s)	454
BstC8I	GCNNGC	5 cut(s)	121, 244, 360, 435, 588
BstDEI	CTNAG	2 cut(s)	564, 570
BstF5I	GGATG	1 cut(s)	457
BstKTI	GATC	2 cut(s)	14, 469
BstMAI	GTCTC	2 cut(s)	368, 618
BstMBI	GATC	2 cut(s)	11, 466
BstMCI	CGRYCG	1 cut(s)	157
BstMWI	GCNNNNNNNGC	4 cut(s)	105, 430, 454, 494
BstNI	CCWGG	1 cut(s)	441
BstNSI	RCATGY	3 cut(s)	437, 590, 604
BstSCI	CCNGG	1 cut(s)	439
BstSLI	GKGCMC	1 cut(s)	657
BstV1I	GCAGC	1 cut(s)	497
BstV2I	GAAGAC	1 cut(s)	34
BstX2I	RGATCY	1 cut(s)	11
BstYI	RGATCY	1 cut(s)	11
BsuRI	GGCC	3 cut(s)	89, 224, 272
BtgZI	GCGATG	2 cut(s)	12, 55
BtsCI	GGATG	1 cut(s)	457
BtsIMutI	CAGTG	1 cut(s)	703
BveI	ACCTGC	1 cut(s)	223
Cac8I	GCNNGC	5 cut(s)	121, 244, 360, 435, 588
CciI	TCATGA	1 cut(s)	463
Cfr10I	RCCGGY	2 cut(s)	119, 242
CviAII	CATG	6 cut(s)	434, 464, 494, 560, 587, 601
DdeI	CTNAG	2 cut(s)	564, 570
DpnI	GATC	2 cut(s)	13, 468
DpnII	GATC	2 cut(s)	11, 466
DraI	TTTAAA	1 cut(s)	337
EaeI	YGGCCR	1 cut(s)	87
Eam1104I	CTCTTC	6 cut(s)	62, 409, 497, 576, 611, 627
EarI	CTCTTC	6 cut(s)	62, 409, 497, 576, 611, 627
EciI	GGCGGA	1 cut(s)	114
Eco130I	CCWWGG	3 cut(s)	225, 306, 323
Eco24I	GRGCYC	1 cut(s)	372
Eco57I	CTGAAG	2 cut(s)	494, 635
Eco88I	CYCGRG	1 cut(s)	371
EcoRI	GAATTC	1 cut(s)	350
EcoRII	CCWGG	1 cut(s)	439
EcoT14I	CCWWGG	3 cut(s)	225, 306, 323
EcoT38I	GRGCYC	1 cut(s)	372
ErhI	CCWWGG	3 cut(s)	225, 306, 323
FaeI	CATG	6 cut(s)	437, 467, 497, 563, 590, 604
FatI	CATG	6 cut(s)	433, 463, 493, 559, 586, 600
FblI	GTMKAC	1 cut(s)	168
Fnu4HI	GCNGC	2 cut(s)	283, 486
FokI	GGATG	1 cut(s)	444
FriOI	GRGCYC	1 cut(s)	372
Fsp4HI	GCNGC	2 cut(s)	283, 486
GluI	GCNGC	2 cut(s)	283, 486
GsuI	CTGGAG	2 cut(s)	221, 423
HaeIII	GGCC	3 cut(s)	89, 224, 272
HapII	CCGG	3 cut(s)	120, 243, 384
Hin1II	CATG	6 cut(s)	437, 467, 497, 563, 590, 604
HincII	GTYRAC	1 cut(s)	169
HindII	GTYRAC	1 cut(s)	169
HinfI	GANTC	3 cut(s)	102, 110, 685
HpaII	CCGG	3 cut(s)	120, 243, 384
HphI	GGTGA	3 cut(s)	229, 377, 586
Hpy166II	GTNNAC	3 cut(s)	169, 399, 655
Hpy188I	TCNGA	5 cut(s)	82, 127, 409, 684, 690
Hpy188III	TCNNGA	5 cut(s)	347, 384, 464, 479, 548
Hpy8I	GTNNAC	3 cut(s)	169, 399, 655
Hpy99I	CGWCG	4 cut(s)	170, 173, 281, 397
HpyAV	CCTTC	6 cut(s)	42, 46, 76, 214, 518, 662
HpyCH4III	ACNGT	1 cut(s)	379
HpyCH4V	TGCA	4 cut(s)	253, 457, 485, 655
HpyF10VI	GCNNNNNNNGC	4 cut(s)	105, 430, 454, 494
HpyF3I	CTNAG	2 cut(s)	564, 570
Hsp92II	CATG	6 cut(s)	437, 467, 497, 563, 590, 604
Kpn2I	TCCGGA	1 cut(s)	383
KroI	GCCGGC	2 cut(s)	119, 242
KroNI	GCCGGC	2 cut(s)	121, 244
Kzo9I	GATC	2 cut(s)	11, 466
LguI	GCTCTTC	2 cut(s)	497, 576
LmnI	GCTCC	4 cut(s)	128, 229, 442, 700
Lsp1109I	GCAGC	1 cut(s)	497
LweI	GCATC	2 cut(s)	466, 472
MalI	GATC	2 cut(s)	13, 468
MboI	GATC	2 cut(s)	11, 466
MflI	RGATCY	1 cut(s)	11
MhlI	GDGCHC	3 cut(s)	372, 657, 705
MlsI	TGGCCA	1 cut(s)	89
MluCI	AATT	3 cut(s)	5, 350, 523
MluNI	TGGCCA	1 cut(s)	89
MlyI	GAGTC	1 cut(s)	104
Mox20I	TGGCCA	1 cut(s)	89
MroI	TCCGGA	1 cut(s)	383
MroNI	GCCGGC	2 cut(s)	119, 242
MscI	TGGCCA	1 cut(s)	89
MseI	TTAA	2 cut(s)	336, 420
MslI	CAYNNNNRTG	2 cut(s)	363, 462
Msp20I	TGGCCA	1 cut(s)	89
MspI	CCGG	3 cut(s)	120, 243, 384
MspR9I	CCNGG	1 cut(s)	441
MvaI	CCWGG	1 cut(s)	441
MwoI	GCNNNNNNNGC	4 cut(s)	105, 430, 454, 494
NaeI	GCCGGC	2 cut(s)	121, 244
NdeII	GATC	2 cut(s)	11, 466
NgoMIV	GCCGGC	2 cut(s)	119, 242
NlaIII	CATG	6 cut(s)	437, 467, 497, 563, 590, 604
NlaIV	GGNNCC	2 cut(s)	124, 673
NmeAIII	GCCGAG	1 cut(s)	75
NspI	RCATGY	3 cut(s)	437, 590, 604
OliI	CACNNNNGTG	1 cut(s)	363
PaeI	GCATGC	2 cut(s)	437, 590
PagI	TCATGA	1 cut(s)	463
PaqCI	CACCTGC	1 cut(s)	223
PciSI	GCTCTTC	2 cut(s)	497, 576
PcsI	WCGNNNNNNNCGW	1 cut(s)	168
PdiI	GCCGGC	2 cut(s)	121, 244
PfeI	GAWTC	2 cut(s)	102, 685
PflFI	GACNNNGTC	1 cut(s)	379
PflMI	CCANNNNNTGG	2 cut(s)	262, 312
PkrI	GCNGC	2 cut(s)	284, 487
PleI	GAGTC	1 cut(s)	104
PpsI	GAGTC	1 cut(s)	104
Psp6I	CCWGG	1 cut(s)	439
PspGI	CCWGG	1 cut(s)	439
PspN4I	GGNNCC	2 cut(s)	124, 673
PsuI	RGATCY	1 cut(s)	11
PsyI	GACNNNGTC	1 cut(s)	379
RseI	CAYNNNNRTG	2 cut(s)	363, 462
SalI	GTCGAC	1 cut(s)	167
SapI	GCTCTTC	2 cut(s)	497, 576
SaqAI	TTAA	2 cut(s)	336, 420
SatI	GCNGC	2 cut(s)	283, 486
Sau3AI	GATC	2 cut(s)	11, 466
SchI	GAGTC	1 cut(s)	104
ScrFI	CCNGG	1 cut(s)	441
SduI	GDGCHC	3 cut(s)	372, 657, 705
SfaNI	GCATC	2 cut(s)	466, 472
SgrDI	CGTCGACG	1 cut(s)	167
SmiMI	CAYNNNNRTG	2 cut(s)	363, 462
SmlI	CTYRAG	1 cut(s)	471
SmoI	CTYRAG	1 cut(s)	471
SphI	GCATGC	2 cut(s)	437, 590
Sse9I	AATT	3 cut(s)	5, 350, 523
SsiI	CCGC	2 cut(s)	99, 282
StyD4I	CCNGG	1 cut(s)	439
StyI	CCWWGG	3 cut(s)	225, 306, 323
TaaI	ACNGT	1 cut(s)	379
TaqI	TCGA	4 cut(s)	20, 138, 168, 276
TaqII	GACCGA	1 cut(s)	143
TasI	AATT	3 cut(s)	5, 350, 523
TauI	GCSGC	1 cut(s)	285
TfiI	GAWTC	2 cut(s)	102, 685
Tru1I	TTAA	2 cut(s)	336, 420
Tru9I	TTAA	2 cut(s)	336, 420
TseI	GCWGC	1 cut(s)	485
TspDTI	ATGAA	4 cut(s)	17, 58, 315, 681
Tth111I	GACNNNGTC	1 cut(s)	379
Van91I	CCANNNNNTGG	2 cut(s)	262, 312
VneI	GTGCAC	1 cut(s)	653
XapI	RAATTY	1 cut(s)	350
XceI	RCATGY	3 cut(s)	437, 590, 604
XcmI	CCANNNNNNNNNTGG	2 cut(s)	188, 232
XmiI	GTMKAC	1 cut(s)	168

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.