Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005238

Physical Location & Seq

Genomic Coordinates Forward (+)

4753705 .. 4761456

Gene Structure 7752 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G14270.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5376 bp

ATGGGTACAAGAGATAGTAATAATAGGACATTCTCTGAGATTGTGGGTCTGATTAAATCCTGGCTCCCCTGGCGATCCGAGCCAGCTACTGTGTCCAGGGATTTTTGGATGCCTGATCAGAGCTGTCGTGTATGTTACGAGTGTGATTGTCAGTTTACCCTCATCAATCGTAGGCACCATTGTCGTCATTGTGGGAGAGTTTTCTGTGGCAAGTGTACTGCCAACTCTATTCCTTTTGCTCCCAGCGACTTGAGAACTCCTCGTGAAGACTGGGAGAGGATACGTGTTTGCAATTATTGTTTCAGGCAGTGGGAGCAAGGTGATGGTGGCCCACACGTCTCAAATATCACGGAACTTAGTACTTCACCTTCAGAGACAAGTCTTCTTAGCTCTAAGACCAGTACAACAGCCAATAGTAGTAGCTTCGCTCTTGGCTCCATGCCGGGACTGATTGGTCTTAATCAAAGGGTTCATCATGGTTCTGATGTCAGCTTGCACGGAGTATCTTCAATGGAGACAAGCGTAACCAAGCAAGGCAAAGAAACTTCAAGGAGGAGCAGCTTCATTGCCACAGACGTGGAAGATCCATCTCGCTTTGCTTTAAATAGCATCAGGAGTGATGATGAGTACGACGAGTATGGTGCATACCAGACTGATATTGAGACGAGTCATTCTCCTCGAGCTAATGAATATTATGGTCCAATGGAATACAATGGAATGGGCATTGATGATGTCCCCTGTAAGCATCTTGGTGGAGAAACCGCGGACCAGAAAAGTTTAAGCGGCTCTCCACTCATTCATCAGTGCCTTGAATCTCTGATTCGGGAAGGATCAGAGCAGTTCCAGAAGAAAAGTGAGCATGATGGTCGTGACGAATGTGAAGCTTCTTCTCCAGCAGATATTTCAGATGATCAAGTGGTAGAACCTGTGGATTTCGAGAACAATGGACTTTTGTGGGTTCCACCTGAGCCAGAAAATGAAGAAGACGAGAGAGAAAGTGCTTTGTTTGATGAGGAAGATAACGAGGGAGATGCCTCAGGTGAGTGGGGATACTTACGACCTTCCACTAGCTTTGGAAGTGGAGAGTACCGTGGCGAGGATCGAACAACTGAAGAGCACAAGAAGGCTATGAAGAATGTGGTTGATGGGCACTTTAGGGCTTTGCTTGCACAACTGTTGCAAGTTGAGAACATCTCTGTGAGCGATGAAGAGGGCAAAGAGAGCTGGTTAGAGATTATCACCTCTCTTTCTTGGGAGGCTGCTAACTTATTGAAGCCTGATATGAGCAAAAGTGGAGGAATGGATCCTGGTGGCTATGTCAAAGTAAAGTGCCTAGCTTCTGGATTCCGACATGATAGCATGGTGGTAAAGGGAGTTGTTTGCAAGAAAAATGTGGTTAATAGGAGAATGAGTACGAAGATTGAGAAAGCTCGACTATTGATTCTAGGCGGTGGACTTGAGTATCAAAGGGTCTCAAACCAGTTATCAAGTTTTGATACTTTGTTGCAACAGGAAAAGGATCATCTAAAGATGGCTGTTGCTAAGATTCACGCTGAACGTCCAAATATTCTACTAGTCGAAAAATCAGTTTCTCGATTTGCGCAAGAGTATCTTCTAGCCAAAGACATATCTCTTGTTCTAAACATCAAGAGGCCACTTTTAGACCGCATTGCTCGCTGCACTGGTGCTCAAATAATTCCTTCAGTAGACCACCTCTCCTCTCAAAAGCTGGGTTACTGTGAGAATTTTCGGGTGGATAGGTATCCTGAAGAACATGGTTCAACTGGTCAAGTTGGAAAGAAAGTGGTGAAGACGTTGATGTATTTTGAGCATTGTCCAAAGCCATTAGGCTTTACTATTTTGCTGAGGGGTGCAAATGAAGATGAGTTGAAAAAAGTGAAGCATGTGGTCCAGTATGGAGTTTTTGCAGCTTATCATTTGGCACTCGAAACATCGTTTCTTGCTGATGAGGGAGCCTCACCAGAACTTCCTTTGAATTCCCCGATTACGGTGGCACTTCCAGATAAATCTACGAGCATTGAACGTTCAATATCTACTGTGCCAGGTTTCACGGTCTCAACATATGAAAAATCTCCGACAATGCTATCTTGTGCTGAACCACAACGAGCAAACAGTGTTCCAGTATCAGAGTTGCTCTCTACCACTACCAATCTATCCATCCAGAAGGATATCCCTCCTATACCCTATGGTTCAGGCTGGCAGGCTAGAGAAATAAACCCGAGTTTCGTCTTTTCACGCCATAATATTTCATTGAATTTGCCTGACCGTGTGATAGAGAGCAGAAACTCAGATTTGTCTGGAAGATCTGTACCGGTGGACACACCAGCTGACAAAAGCAATCCAATTGTTGTGGCAGATGAGACAACGAACAATTCCTTACATTTATCAGGGCAAGGCTTTGTGCGAAAGAGTTCTCAAATTGGTACGAGCATTATGGTCGAAAACCAAGACAATGGTTCAGAGCTTACAATCGCCCAACAACAAAACAATGAGAAACCAAAGGAAACACAATCCCAAAAAGAAGAATTTCCTCCGTCACCCTCTGACCACCAGAGCATTTTGGTTTCTCTATCATCCCGATCAGTGTGGAAAGGAACTGTGTGTGAAAGGTCTCATCTCTTTCGGATAAAATACTATGGTAGTTTTGATAAACCCTTGGGACGGTTCTTGAGAGATCATTTGTTCGATCAGAGTTACAGGTGCCGGTCATGTGAGATGCCATCAGAAGCTCATGTTCACTGTTACACTCATCGACAAGGCAGCCTTACGATATCTGTCAAGAAACTTCAAGATTATCTCTTACCTGGTGAAAAGGAGGGGAAGATCTGGATGTGGCATAGATGCCTGAGATGCCCGCGACTTAACGGCTTCCCTCCAGCGACTCTACGAGTGGTTATGTCTGATGCTGCATGGGGATTATCGTTTGGGAAGTTTCTGGAGCTCAGTTTCTCAAATCACGCAGCTGCTAGTAGAGTAGCTTGTTGTGGCCATTCTCTGCATAGAGATTGTCTTCGCTTCTATGGATTTGGGAACATGGTTGCTTGCTTCCGGTACGCTACTATAGATGTTCATTCGGTCTACCTTCCACCATCAATTCTTAGTTTCAACTACGAGAATCAGGATTGGATACAGAGAGAGACAGACGAGGTGATAGAGAGAGCAGAGCTTCTGTTTTCTGAAGTATTAAATGCTATTAGTCAAATTGCAGAGAAAGGTTTCAGGCGACGAATTGGTGAACTCGAAGAAGTGCTGCAAAAAGAGAAAGCGGAATTTGAGGAGAATATGCAAAAGATTTTGCACAGGGAGGTGAACGAAGGCCAACCTCTCGTGGACATTCTTGAGCTATATCGGATTCACAGACAACTTCTTTTTCAGTCATACATGTGGGATCACCGCTTAATTAATGCATCTACTTTACATAAGCTTGAGAATAGTGATGACACAAAGAGAGAAGAGAACGAGAAACCTCCCTTGGCTAAGAGCCAGACGCTCCCGGAGATGAATGCTGGGACCAACTCTCTTCTTACTGGCTCTGAGGTTAATCTGAATCCTGATGGTGATTCTACTGGTGACACTGGCTCCTTAAATAATGTTCAGAAAGAAGCGGATACGAATTCAGATTTGTATCAGGAAAAGGATGATGGGGGAGAAGTTTCTCCCAGCAAGACCTTACCAGATACATCTTATCCTCTGGAAAATAAAGTCGATGTTCGTAGAACACAGTCTGATGGCCAGATAGTTATGAAAAATCTATCAGCCACTCTTGATGCAGCATGGATAGGTGAACGTCAAACATCTGTGGAGATTCCAACCAACAATAAGGTCTCGCTTCCTCCTTCCACCATGTCAAACTCTTCAACTTTTCCTCCAATCTCAGAGGGGCTAATGCCTATAGATCTCCCAGAACAGCAAAATGAGTTCAAGGTGGCCTATCCTGTTTCCCCAGCATTACCGAGTAAGAATTATGAGAATTCAGAAGACTCTGTCAGCTGGCTAAGTGTGCCATTCCTCAACTTCTACCGTTCCATTAACAAGAATTTCCTGCTTAGCTCTCAGAAGCTTGATACATTTGGCGAGCATAGCCCTATCTATATTTCATCTTTTAGAGAGGCAGAGCTTCAAGGTGGACCAAGGCTACTTCTGCCCGTAGGCTTAAATGATATTGTTGTTCCGGTTTATGATGATGAACCTACTAGTATGATCGCATATGCCTTAATGTCACCAGAATATCAACGCCAAACTTCTGCTGAAGGGGAATCTCTAGTTTCATATCCTTCTGAACTCAATATCCCTCGCCCAGTTGATGATACCATTTTTGACCCTAGCAGAAGCAATGGTTCAGTCGACGAGAGCATACTTTCAATATCCTCATCTCGCAGTACCTCACTTCTGGACCCACTATCATATACAAAAGCTTTACATGCTCGAGTCTCATATGGAGAGGACGGTACGCTTGGAAAAGTGAAGTACACTGTTACTTGTTACTATGCAAAGCGGTTTGAGGCGCTACGGGGTATATGTCTCCCTTCGGAACTTGAGTATATACGGTCTCTTAGCCGGTGTAAAAAATGGGGAGCTCAAGGTGGCAAGAGCAATGTCTTCTTTGCTAAAACCCTGGACGATCGATTTATCATCAAGCAAGTCACCAAGACAGAGTTGGAATCATTCATCAAATTTGCACCTGCTTACTTTAAATACTTGTCTGAGTCTATCAGCACGAAAAGCCCAACCTGCCTGGCAAAAATCTTGGGAATCTATCAGGTCGCAACAAAGCAACTTAAGAGCGGGAAAGAAACAAAGATGGATGTTCTGATTATGGAGAATCTTCTGTTTGGAAGAACTGTGAAACGGCTTTATGACCTTAAAGGATCTTCGCGGGCGCGCTACAATCCTGACTCTAGTGGGAGCAACAAAGTCTTGTTGGATCAAAACTTGATTGAAGCAATGCCAACTTCTCCCATTTTCGTCGGTAACAAAGCAAAACGATTGTTGGAAAGAGCTGTCTGGAACGATACTGCTTTTCTTGCATTGGGTGATGTGATGGATTATTCATTGCTAGTAGGCGTGGATGAAGAAAAGAACGAGCTAGTTCTCGGGATTATCGACTTCTTGAGGCAGTACACATGGGACAAGCATCTAGAGTCTTGGGTGAAATTTACAGGAATCTTAGGAGGACCAAAGAATGAAGCACCAACAGTAATCTCTCCAAAGCAATATAAGAGAAGATTCAGAAAAGCAATGACAACTTACTTCCTCATGGTCCCTGATCAATGGTCTCCGCCTAATGTTGTTGCAAATAACTCCAAGTCTGATCAACCTGAAGAAACCTCTCAAGCCGGTACACAAGCAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000285 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005768 GO:0005773 GO:0005774 GO:0005942 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006810 GO:0006996 GO:0007033 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009555 GO:0009987 GO:0010008 GO:0010118 GO:0010256 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016301 GO:0016307 GO:0016310 GO:0016482 GO:0016740 GO:0016772 GO:0016773 GO:0019637 GO:0019898 GO:0030258 GO:0031090 GO:0031410 GO:0031982 GO:0032266 GO:0032501 GO:0032502 GO:0032991 GO:0035032 GO:0035091 GO:0042147 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0048229 GO:0048856 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061695 GO:0070772 GO:0071704 GO:0071840 GO:0090332 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:1901981 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00562 ko04070 ko04145 ko04810 map00562 map04070 map04145 map04810

Pfam Domains

Protein Families

Cpn60_TCP1 FYVE PIP5K

Protein Analysis

1791

Amino Acids

200.83

Weight (kDa)

5.62

Isoelectric Point (pI)

49.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FYVE	PF01363	36 - 104	4.9e-16	FYVE zinc finger
Cpn60_TCP1	PF00118	391 - 653	2.5e-32	TCP-1/cpn60 chaperonin family
PIP5K	PF01504	1534 - 1700	1.5e-32	Phosphatidylinositol-4-phosphate 5-Kinase
PIP5K	PF01504	1700 - 1756	7.1e-08	Phosphatidylinositol-4-phosphate 5-Kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0005238)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G14270 AT3G14270
fragaria_vesca	FvH4_6g35770 FvH4_6g35770 FvH4_6g35770
malus_domestica	MD09G1173300.v1.1 MD17G1154000.v1.1
prunus_persica	Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1
pyrus_communis	pycom09g09070 pycom17g14660
rosa_chinensis	RchiOBHm_Chr2g0147811
rosa_laevigata	RLG00000020280
rosa_multiflora	Rmu_sc0000308.1_g000046
rosa_roxburghii	Rroxscaffold_2G00099330
rosa_rugosa	Rorug02G0401800 Rorug02G0401800 Rorug02G0401800 Rorug02G0401900
rosa_samantha	Rh2AG459000 Rh2BG472300 Rh2CG446400 Rh2DG481300
rosa_wichuraiana	Rw2G037540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	4711
AasI	GACNNNNNNGTC	1 cut(s)	453
Acc16I	TGCGCA	1 cut(s)	1602
Acc36I	ACCTGC	2 cut(s)	4711, 4760
AccB1I	GGYRCC	2 cut(s)	174, 2721
AccBSI	CCGCTC	1 cut(s)	4806
AccI	GTMKAC	3 cut(s)	1707, 3099, 4365
AccII	CGCG	4 cut(s)	764, 2878, 4897, 4903
AclI	AACGTT	1 cut(s)	2044
AcoI	YGGCCR	2 cut(s)	3007, 3751
AcuI	CTGAAG	7 cut(s)	354, 1131, 1686, 1788, 3219, 4290, 5361
AfiI	CCNNNNNNNGG	3 cut(s)	1096, 2008, 2682
AflII	CTTAAG	1 cut(s)	4799
AflIII	ACRYGT	3 cut(s)	283, 334, 3402
AgeI	ACCGGT	1 cut(s)	2332
AhlI	ACTAGT	2 cut(s)	1573, 4214
AjiI	CACGTC	2 cut(s)	337, 577
AjnI	CCWGG	8 cut(s)	59, 68, 95, 1306, 2062, 2824, 4635, 4755
AjuI	GAANNNNNNNTTGG	2 cut(s)	3476, 3508
AleI	CACNNNNGTG	1 cut(s)	575
AloI	GAACNNNNNNTCC	2 cut(s)	4293, 4325
Alw21I	GWGCWC	4 cut(s)	1119, 1690, 2964, 4600
AlwNI	CAGNNNCTG	1 cut(s)	89
Ama87I	CYCGRG	4 cut(s)	678, 2239, 4446, 5114
AoxI	GGCC	6 cut(s)	328, 1652, 3007, 3337, 3751, 3948
AseI	ATTAAT	1 cut(s)	3423
AsiGI	ACCGGT	1 cut(s)	2332
Asp700I	GAANNNNTTC	3 cut(s)	2431, 2547, 3236
AspLEI	GCGC	4 cut(s)	1603, 4528, 4903, 4905
AspS9I	GGNCC	9 cut(s)	329, 698, 766, 1909, 3531, 4148, 4414, 5195, 5281
AsuC2I	CCSGG	2 cut(s)	444, 3515
AvaI	CYCGRG	4 cut(s)	678, 2239, 4446, 5114
AvaII	GGWCC	8 cut(s)	698, 766, 1909, 3531, 4148, 4414, 5195, 5281
AxyI	CCTNAGG	1 cut(s)	1036
BaeGI	GKGCMC	1 cut(s)	1152
BaeI	ACNNNNGTAYC	2 cut(s)	1445, 1478
BalI	TGGCCA	2 cut(s)	3009, 3753
BamHI	GGATCC	1 cut(s)	1303
BanI	GGYRCC	2 cut(s)	174, 2721
BanII	GRGCYC	2 cut(s)	2964, 4600
BarI	GAAGNNNNNNTAC	2 cut(s)	352, 384
BauI	CACGAG	2 cut(s)	261, 3347
BbsI	GAAGAC	7 cut(s)	273, 374, 990, 1817, 3023, 4005, 4612
Bbv12I	GWGCWC	4 cut(s)	1119, 1690, 2964, 4600
BbvCI	CCTCAGC	1 cut(s)	1865
BceAI	ACGGC	2 cut(s)	2902, 4886
BciT130I	CCWGG	8 cut(s)	61, 70, 97, 1308, 2064, 2826, 4637, 4757
BciVI	GTATCC	5 cut(s)	273, 1043, 1773, 3141, 3622
BclI	TGATCA	4 cut(s)	115, 910, 5287, 5332
BcnI	CCSGG	2 cut(s)	444, 3515
BcuI	ACTAGT	2 cut(s)	1573, 4214
BfmI	CTRYAG	2 cut(s)	3081, 3912
BfoI	RGCGCY	1 cut(s)	4529
BfrI	CTTAAG	1 cut(s)	4799
BfuAI	ACCTGC	2 cut(s)	4711, 4760
BfuI	GTATCC	5 cut(s)	273, 1043, 1773, 3141, 3622
BglII	AGATCT	3 cut(s)	2324, 2844, 3916
BlpI	GCTNAGC	1 cut(s)	4067
BmcAI	AGTACT	1 cut(s)	361
Bme18I	GGWCC	8 cut(s)	698, 766, 1909, 3531, 4148, 4414, 5195, 5281
BmeT110I	CYCGRG	4 cut(s)	678, 2239, 4446, 5114
BmgBI	CACGTC	2 cut(s)	337, 577
BmgT120I	GGNCC	9 cut(s)	329, 698, 766, 1909, 3531, 4148, 4414, 5195, 5281
BmrI	ACTGGG	2 cut(s)	280, 4313
BmuI	ACTGGG	2 cut(s)	280, 4313
BpiI	GAAGAC	7 cut(s)	273, 374, 990, 1817, 3023, 4005, 4612
BpmI	CTGGAG	3 cut(s)	876, 2880, 2978
Bpu10I	CCTNAGC	2 cut(s)	966, 1865
Bpu1102I	GCTNAGC	1 cut(s)	4067
BpuEI	CTTGAG	9 cut(s)	271, 1478, 2710, 3380, 3467, 4577, 4584, 5152, 5337
BpuMI	CCSGG	2 cut(s)	444, 3515
Bsa29I	ATCGAT	1 cut(s)	4645
BsaAI	YACGTR	1 cut(s)	284
BsaBI	GATNNNNATC	2 cut(s)	1761, 3645
BsaI	GGTCTC	6 cut(s)	1477, 2080, 2637, 3850, 4575, 5301
BsaJI	CCNNGG	8 cut(s)	68, 96, 762, 1090, 2676, 3492, 4151, 4635
BsaWI	WCCGGW	3 cut(s)	2332, 3069, 4192
BsaXI	ACNNNNNCTCC	8 cut(s)	1396, 1426, 1700, 1730, 3584, 3614, 3871, 3901
Bsc4I	CCNNNNNNNGG	3 cut(s)	1096, 2008, 2682
Bse118I	RCCGGY	4 cut(s)	2332, 2724, 4578, 5357
Bse21I	CCTNAGG	1 cut(s)	1036
Bse3DI	GCAATG	7 cut(s)	564, 1668, 4360, 4621, 4971, 5072, 5265
Bse8I	GATNNNNATC	2 cut(s)	1761, 3645
BseBI	CCWGG	8 cut(s)	61, 70, 97, 1308, 2064, 2826, 4637, 4757
BseCI	ATCGAT	1 cut(s)	4645
BseDI	CCNNGG	8 cut(s)	68, 96, 762, 1090, 2676, 3492, 4151, 4635
BseGI	GGATG	7 cut(s)	114, 2178, 2594, 2856, 3664, 4831, 5095
BseJI	GATNNNNATC	2 cut(s)	1761, 3645
BseLI	CCNNNNNNNGG	3 cut(s)	1096, 2008, 2682
BseMI	GCAATG	7 cut(s)	564, 1668, 4360, 4621, 4971, 5072, 5265
BsePI	GCGCGC	1 cut(s)	4901
BseRI	GAGGAG	5 cut(s)	249, 568, 666, 1708, 3311
BseSI	GKGCMC	1 cut(s)	1152
BseYI	CCCAGC	5 cut(s)	242, 1729, 3527, 3680, 3964
BsgI	GTGCAG	1 cut(s)	1663
Bsh1236I	CGCG	4 cut(s)	764, 2878, 4897, 4903
Bsh1285I	CGRYCG	1 cut(s)	4645
BshFI	GGCC	6 cut(s)	330, 1654, 3009, 3339, 3753, 3950
BshNI	GGYRCC	2 cut(s)	174, 2721
BshTI	ACCGGT	1 cut(s)	2332
BshVI	ATCGAT	1 cut(s)	4645
BsiEI	CGRYCG	1 cut(s)	4645
BsiHKAI	GWGCWC	4 cut(s)	1119, 1690, 2964, 4600
BsiHKCI	CYCGRG	4 cut(s)	678, 2239, 4446, 5114
BsiSI	CCGG	8 cut(s)	443, 2333, 2725, 3070, 3515, 4193, 4579, 5358
BslFI	GGGAC	6 cut(s)	459, 719, 2694, 3544, 5162, 5267
BslI	CCNNNNNNNGG	3 cut(s)	1096, 2008, 2682
BsmBI	CGTCTC	2 cut(s)	343, 656
BsmFI	GGGAC	6 cut(s)	459, 719, 2694, 3544, 5162, 5267
BsmI	GAATGC	1 cut(s)	3529
BsnI	GGCC	6 cut(s)	330, 1654, 3009, 3339, 3753, 3950
Bso31I	GGTCTC	6 cut(s)	1477, 2080, 2637, 3850, 4575, 5301
BsoBI	CYCGRG	4 cut(s)	678, 2239, 4446, 5114
Bsp1286I	GDGCHC	5 cut(s)	1119, 1152, 1690, 2964, 4600
Bsp1720I	GCTNAGC	1 cut(s)	4067
BspANI	GGCC	6 cut(s)	330, 1654, 3009, 3339, 3753, 3950
BspDI	ATCGAT	1 cut(s)	4645
BspFNI	CGCG	4 cut(s)	764, 2878, 4897, 4903
BspMI	ACCTGC	2 cut(s)	4711, 4760
BspQI	GCTCTTC	1 cut(s)	1107
BspT107I	GGYRCC	2 cut(s)	174, 2721
BspTI	CTTAAG	1 cut(s)	4799
BspTNI	GGTCTC	6 cut(s)	1477, 2080, 2637, 3850, 4575, 5301
BsrBI	CCGCTC	1 cut(s)	4806
BsrDI	GCAATG	7 cut(s)	564, 1668, 4360, 4621, 4971, 5072, 5265
BsrFI	RCCGGY	4 cut(s)	2332, 2724, 4578, 5357
BssAI	RCCGGY	4 cut(s)	2332, 2724, 4578, 5357
BssECI	CCNNGG	8 cut(s)	68, 96, 762, 1090, 2676, 3492, 4151, 4635
BssHII	GCGCGC	1 cut(s)	4901
BssSI	CACGAG	2 cut(s)	261, 3347
BssT1I	CCWWGG	3 cut(s)	2676, 3492, 4151
Bst2BI	CACGAG	2 cut(s)	261, 3347
Bst2UI	CCWGG	8 cut(s)	61, 70, 97, 1308, 2064, 2826, 4637, 4757
Bst6I	CTCTTC	5 cut(s)	1107, 1203, 3468, 3546, 3880
BstAFI	CTTAAG	1 cut(s)	4799
BstBAI	YACGTR	1 cut(s)	284
BstDSI	CCRYGG	2 cut(s)	762, 1090
BstF5I	GGATG	7 cut(s)	114, 2178, 2594, 2856, 3664, 4831, 5095
BstFNI	CGCG	4 cut(s)	764, 2878, 4897, 4903
BstH2I	RGCGCY	1 cut(s)	4529
BstHHI	GCGC	4 cut(s)	1603, 4528, 4903, 4905
BstMCI	CGRYCG	1 cut(s)	4645
BstNI	CCWGG	8 cut(s)	61, 70, 97, 1308, 2064, 2826, 4637, 4757
BstNSI	RCATGY	3 cut(s)	1907, 3406, 4445
BstSFI	CTRYAG	2 cut(s)	3081, 3912
BstSLI	GKGCMC	1 cut(s)	1152
BstUI	CGCG	4 cut(s)	764, 2878, 4897, 4903
BstV2I	GAAGAC	7 cut(s)	273, 374, 990, 1817, 3023, 4005, 4612
BstX2I	RGATCY	6 cut(s)	583, 1303, 2324, 2844, 3916, 4889
BstXI	CCANNNNNNTGG	2 cut(s)	577, 2474
BstYI	RGATCY	6 cut(s)	583, 1303, 2324, 2844, 3916, 4889
Bsu15I	ATCGAT	1 cut(s)	4645
Bsu36I	CCTNAGG	1 cut(s)	1036
BsuI	GTATCC	5 cut(s)	273, 1043, 1773, 3141, 3622
BsuRI	GGCC	6 cut(s)	330, 1654, 3009, 3339, 3753, 3950
BsuTUI	ATCGAT	1 cut(s)	4645
BtgI	CCRYGG	2 cut(s)	762, 1090
BtgZI	GCGATG	1 cut(s)	1218
BtrI	CACGTC	2 cut(s)	337, 577
BtsCI	GGATG	7 cut(s)	114, 2178, 2594, 2856, 3664, 4831, 5095
BtsI	GCAGTG	1 cut(s)	314
BtsIMutI	CAGTG	8 cut(s)	314, 809, 1680, 2140, 2610, 2758, 3594, 4491
BveI	ACCTGC	2 cut(s)	4711, 4760
CaiI	CAGNNNCTG	1 cut(s)	89
CfoI	GCGC	4 cut(s)	1603, 4528, 4903, 4905
Cfr10I	RCCGGY	4 cut(s)	2332, 2724, 4578, 5357
Cfr13I	GGNCC	9 cut(s)	329, 698, 766, 1909, 3531, 4148, 4414, 5195, 5281
Cfr42I	CCGCGG	1 cut(s)	765
ClaI	ATCGAT	1 cut(s)	4645
CseI	GACGC	1 cut(s)	3517
CsiI	ACCWGGT	1 cut(s)	2824
CspAI	ACCGGT	1 cut(s)	2332
CspCI	CAANNNNNGTGG	4 cut(s)	1054, 1089, 2352, 2387
DraI	TTTAAA	2 cut(s)	603, 4714
DrdI	GACNNNNNNGTC	1 cut(s)	453
DseDI	GACNNNNNNGTC	1 cut(s)	453
EaeI	YGGCCR	2 cut(s)	3007, 3751
Eam1104I	CTCTTC	5 cut(s)	1107, 1203, 3468, 3546, 3880
EarI	CTCTTC	5 cut(s)	1107, 1203, 3468, 3546, 3880
EciI	GGCGGA	1 cut(s)	5289
Ecl136II	GAGCTC	2 cut(s)	2962, 4598
Eco130I	CCWWGG	3 cut(s)	2676, 3492, 4151
Eco24I	GRGCYC	2 cut(s)	2964, 4600
Eco31I	GGTCTC	6 cut(s)	1477, 2080, 2637, 3850, 4575, 5301
Eco32I	GATATC	2 cut(s)	2191, 2793
Eco47I	GGWCC	8 cut(s)	698, 766, 1909, 3531, 4148, 4414, 5195, 5281
Eco53kI	GAGCTC	2 cut(s)	2962, 4598
Eco57I	CTGAAG	7 cut(s)	354, 1131, 1686, 1788, 3219, 4290, 5361
Eco81I	CCTNAGG	1 cut(s)	1036
Eco88I	CYCGRG	4 cut(s)	678, 2239, 4446, 5114
EcoICRI	GAGCTC	2 cut(s)	2962, 4598
EcoRI	GAATTC	3 cut(s)	1996, 3634, 3991
EcoRII	CCWGG	8 cut(s)	59, 68, 95, 1306, 2062, 2824, 4635, 4755
EcoRV	GATATC	2 cut(s)	2191, 2793
EcoT14I	CCWWGG	3 cut(s)	2676, 3492, 4151
EcoT22I	ATGCAT	1 cut(s)	3430
EcoT38I	GRGCYC	2 cut(s)	2964, 4600
ErhI	CCWWGG	3 cut(s)	2676, 3492, 4151
Esp3I	CGTCTC	2 cut(s)	343, 656
FalI	AAGNNNNNCTT	4 cut(s)	1596, 1628, 2769, 2801
FaqI	GGGAC	6 cut(s)	459, 719, 2694, 3544, 5162, 5267
FauI	CCCGC	3 cut(s)	2883, 4799, 4890
FauNDI	CATATG	3 cut(s)	2083, 4228, 4456
FbaI	TGATCA	4 cut(s)	115, 910, 5287, 5332
FblI	GTMKAC	3 cut(s)	1707, 3099, 4365
FokI	GGATG	7 cut(s)	121, 2165, 2581, 2863, 3671, 4838, 5102
FriOI	GRGCYC	2 cut(s)	2964, 4600
FspI	TGCGCA	1 cut(s)	1602
GlaI	GCGC	4 cut(s)	1602, 4527, 4902, 4904
GsaI	CCCAGC	5 cut(s)	246, 1733, 3531, 3684, 3968
GsuI	CTGGAG	3 cut(s)	876, 2880, 2978
HaeII	RGCGCY	1 cut(s)	4529
HaeIII	GGCC	6 cut(s)	330, 1654, 3009, 3339, 3753, 3950
HapII	CCGG	8 cut(s)	443, 2333, 2725, 3070, 3515, 4193, 4579, 5358
HgaI	GACGC	1 cut(s)	3517
HhaI	GCGC	4 cut(s)	1603, 4528, 4903, 4905
Hin6I	GCGC	4 cut(s)	1601, 4526, 4901, 4903
HinP1I	GCGC	4 cut(s)	1601, 4526, 4901, 4903
HincII	GTYRAC	1 cut(s)	4366
HindII	GTYRAC	1 cut(s)	4366
HindIII	AAGCTT	4 cut(s)	882, 3443, 4079, 4434
HpaII	CCGG	8 cut(s)	443, 2333, 2725, 3070, 3515, 4193, 4579, 5358
Hpy99I	CGWCG	4 cut(s)	635, 3249, 4370, 4991
HpyCH4IV	ACGT	7 cut(s)	283, 336, 576, 1558, 1814, 2044, 3808
HpySE526I	ACGT	7 cut(s)	283, 336, 576, 1558, 1814, 2044, 3808
HspAI	GCGC	4 cut(s)	1601, 4526, 4901, 4903
Ksp22I	TGATCA	4 cut(s)	115, 910, 5287, 5332
KspI	CCGCGG	1 cut(s)	765
LguI	GCTCTTC	1 cut(s)	1107
MabI	ACCWGGT	1 cut(s)	2824
MaeII	ACGT	7 cut(s)	283, 336, 576, 1558, 1814, 2044, 3808
MbiI	CCGCTC	1 cut(s)	4806
MfeI	CAATTG	1 cut(s)	2364
MflI	RGATCY	6 cut(s)	583, 1303, 2324, 2844, 3916, 4889
MhlI	GDGCHC	5 cut(s)	1119, 1152, 1690, 2964, 4600
MlsI	TGGCCA	2 cut(s)	3009, 3753
MluNI	TGGCCA	2 cut(s)	3009, 3753
MlyI	GAGTC	7 cut(s)	676, 2896, 3995, 4458, 4736, 4910, 5171
MmeI	TCCRAC	7 cut(s)	1372, 1774, 2120, 3854, 4659, 4923, 4992
Mox20I	TGGCCA	2 cut(s)	3009, 3753
Mph1103I	ATGCAT	1 cut(s)	3430
MroXI	GAANNNNTTC	3 cut(s)	2431, 2547, 3236
MscI	TGGCCA	2 cut(s)	3009, 3753
MslI	CAYNNNNRTG	4 cut(s)	575, 750, 1196, 3403
Msp20I	TGGCCA	2 cut(s)	3009, 3753
MspA1I	CMGCKG	4 cut(s)	764, 2348, 2984, 4011
MspCI	CTTAAG	1 cut(s)	4799
MspI	CCGG	8 cut(s)	443, 2333, 2725, 3070, 3515, 4193, 4579, 5358
MunI	CAATTG	1 cut(s)	2364
Mva1269I	GAATGC	1 cut(s)	3529
MvaI	CCWGG	8 cut(s)	61, 70, 97, 1308, 2064, 2826, 4637, 4757
MvnI	CGCG	4 cut(s)	764, 2878, 4897, 4903
NciI	CCSGG	2 cut(s)	444, 3515
NdeI	CATATG	3 cut(s)	2083, 4228, 4456
NmuCI	GTSAC	5 cut(s)	869, 2556, 3590, 4239, 4663
NsbI	TGCGCA	1 cut(s)	1602
NsiI	ATGCAT	1 cut(s)	3430
NspI	RCATGY	3 cut(s)	1907, 3406, 4445
OliI	CACNNNNGTG	1 cut(s)	575
PacI	TTAATTAA	1 cut(s)	3423
PaeR7I	CTCGAG	2 cut(s)	678, 4446
PaqCI	CACCTGC	1 cut(s)	4711
PauI	GCGCGC	1 cut(s)	4901
PciI	ACATGT	1 cut(s)	3402
PciSI	GCTCTTC	1 cut(s)	1107
PcsI	WCGNNNNNNNCGW	1 cut(s)	5121
PctI	GAATGC	1 cut(s)	3529
PdmI	GAANNNNTTC	3 cut(s)	2431, 2547, 3236
PflFI	GACNNNGTC	1 cut(s)	4004
PfoI	TCCNGGA	1 cut(s)	3513
PinAI	ACCGGT	1 cut(s)	2332
Ple19I	CGATCG	1 cut(s)	4645
PleI	GAGTC	7 cut(s)	675, 2896, 3995, 4457, 4735, 4910, 5170
PpsI	GAGTC	7 cut(s)	675, 2896, 3995, 4457, 4735, 4910, 5170
Ppu21I	YACGTR	1 cut(s)	284
PscI	ACATGT	1 cut(s)	3402
PshBI	ATTAAT	1 cut(s)	3423
Psp124BI	GAGCTC	2 cut(s)	2964, 4600
Psp1406I	AACGTT	1 cut(s)	2044
Psp6I	CCWGG	8 cut(s)	59, 68, 95, 1306, 2062, 2824, 4635, 4755
PspFI	CCCAGC	5 cut(s)	242, 1729, 3527, 3680, 3964
PspGI	CCWGG	8 cut(s)	59, 68, 95, 1306, 2062, 2824, 4635, 4755
PspPI	GGNCC	9 cut(s)	329, 698, 766, 1909, 3531, 4148, 4414, 5195, 5281
PspXI	VCTCGAGB	2 cut(s)	678, 4446
PstNI	CAGNNNCTG	1 cut(s)	89
PsuI	RGATCY	6 cut(s)	583, 1303, 2324, 2844, 3916, 4889
PsyI	GACNNNGTC	1 cut(s)	4004
PteI	GCGCGC	1 cut(s)	4901
PvuI	CGATCG	1 cut(s)	4645
PvuII	CAGCTG	3 cut(s)	2348, 2984, 4011
RseI	CAYNNNNRTG	4 cut(s)	575, 750, 1196, 3403
SacI	GAGCTC	2 cut(s)	2964, 4600
SacII	CCGCGG	1 cut(s)	765
SalI	GTCGAC	1 cut(s)	4364
SapI	GCTCTTC	1 cut(s)	1107
Sau96I	GGNCC	9 cut(s)	329, 698, 766, 1909, 3531, 4148, 4414, 5195, 5281
ScaI	AGTACT	1 cut(s)	361
SchI	GAGTC	7 cut(s)	676, 2896, 3995, 4458, 4736, 4910, 5171
SduI	GDGCHC	5 cut(s)	1119, 1152, 1690, 2964, 4600
SexAI	ACCWGGT	1 cut(s)	2824
SfcI	CTRYAG	2 cut(s)	3081, 3912
Sfr274I	CTCGAG	2 cut(s)	678, 4446
Sfr303I	CCGCGG	1 cut(s)	765
SgrBI	CCGCGG	1 cut(s)	765
SinI	GGWCC	8 cut(s)	698, 766, 1909, 3531, 4148, 4414, 5195, 5281
SlaI	CTCGAG	2 cut(s)	678, 4446
SmiMI	CAYNNNNRTG	4 cut(s)	575, 750, 1196, 3403
SpeI	ACTAGT	2 cut(s)	1573, 4214
SspI	AATATT	3 cut(s)	692, 1567, 2266
SstI	GAGCTC	2 cut(s)	2964, 4600
StyI	CCWWGG	3 cut(s)	2676, 3492, 4151
TaiI	ACGT	7 cut(s)	286, 339, 579, 1561, 1817, 2047, 3811
TaqII	GACCGA	1 cut(s)	3085
TatI	WGTACW	5 cut(s)	215, 359, 401, 4488, 5139
TauI	GCSGC	1 cut(s)	786
TscAI	CASTG	8 cut(s)	314, 809, 1687, 2140, 2610, 2765, 3601, 4498
TseFI	GTSAC	5 cut(s)	869, 2556, 3590, 4239, 4663
Tsp45I	GTSAC	5 cut(s)	869, 2556, 3590, 4239, 4663
TspGWI	ACGGA	3 cut(s)	365, 513, 2544
TspRI	CASTG	8 cut(s)	314, 809, 1687, 2140, 2610, 2765, 3601, 4498
Tth111I	GACNNNGTC	1 cut(s)	4004
Vha464I	CTTAAG	1 cut(s)	4799
VpaK11BI	GGWCC	8 cut(s)	698, 766, 1909, 3531, 4148, 4414, 5195, 5281
VspI	ATTAAT	1 cut(s)	3423
XbaI	TCTAGA	1 cut(s)	5158
XceI	RCATGY	3 cut(s)	1907, 3406, 4445
XcmI	CCANNNNNNNNNTGG	1 cut(s)	4675
XhoI	CTCGAG	2 cut(s)	678, 4446
XmiI	GTMKAC	3 cut(s)	1707, 3099, 4365
XmnI	GAANNNNTTC	3 cut(s)	2431, 2547, 3236
ZrmI	AGTACT	1 cut(s)	361
Zsp2I	ATGCAT	1 cut(s)	3430

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.