Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0005238

Physical Location & Seq

Genomic Coordinates Forward (+)

14538935 .. 14547970

Gene Structure 9036 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1154000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5505 bp

ATGGCTGCGCCCAGCAAGATATTCTCCCATTTTATTTCCATGGTTAAATCATGGATCCCTTGGCGATCAGAGCCTGCTAATGTTTCGAGGGATTTTTGGATGCCTGATCATAGCTGTAGGGTATGCTACGAGTGTGATTCACAGTTTACTGTCTTTAACCGTAAACATCATTGTCGACTTTGTGGACGAGTTTTCTGTGCTAAGTGTACAGAGAACTCAATACCTGCCCCCTCTGGTGACCCAAGGACAGATCGGGAAGATTGGGAGAAGATTCGTGTATGCAATTTTTGTTACAAGCAACGGGAGCAAGGCATAGTTATTCCTGATAACGGAATCTCGGTTGCCAACTTAGATCTTAGTACTTCGCCATCAGAAACCAGTTTTGCTAGCTTTAAGTCATGTGGCACTGGTAGCAGTAGTAGCTTCACTAACTCAATGCCATACTCAACTGGGCCATATCAACAATTTCAACTTGGTTCTGGCCTCAGCCCCTGTCAATCGTCTCTAATGGAAACTAACACAGAAAAGCAGAGCAAATTCGGTCAATGGAAGAACAGTGATTTTGTTGCCGATATGTCTCCAGATCAATATGAAGTTGCGACTGCCAGGAGTGATGACGAGGATGTTGAGTATGGTGTATATCAGTCAGATTCAAAGAACTATTCTCAAGTCAGTGACTACTTTAGTCACATTGATTTCGATGAGATGAGCAATGATGATGGATCGCATAAGGTGCATCTTGATGGAGAAAATATTGATGTGAAAAATTTAAGCAGCTCCTCGTTACTTCCCAGTCATGACTCGCAGGTTTTGGAGGGAATCCAACAGCTTGAAAAAAAAGAAGATGAACATGATACTGGGGATGAATGTGAGGCATCTTCCTCTATGTATTCTGCAGGGGATGATGATACAGAACCTGTGGATTTTGAGAACAATGGACTTTTATGGCTTCCCCCTGAACCAGAAGATGAAGAAGATGAAAAGGAAACTGTTCTACTTGATGATGATGATGATGGTGACGCTACAGGAGAGTGGGGGCATTTACGTGCATCAAGCAGTTTTGGAAGTGGGGAGTATCGCAATAGGGATCGGTCAGGTGAGGAGCACAAGAAGGCCATGAAGAATGTAGTTGATGGACACTTTAGGGCTTTGGTAGCTCAGCTGTTGCAGGTTGAGAACCTTCCAATCGGGCAGGAAGGAGAAACAGAAGGTTGGTTGGAGATCATTACGTCTCTGTCCTGGGAGGCTGCTACACTATTAAAGCCAGATATGAGCAAAGGTGGAGGGATGGACCCAGGTGGTTATGTAAAAGTAAAATGTATAGCTTCTGGATCTCGCTGTGATAGTATGGTGGTCAAAGGAGTTGTGTGTAAGAAAAATGTGGCTCATCGACGAATGACATCCAAAATAGAGAAACCTCGGTTTATGATCCTTGGTGGTGCTCTTGAGTACCAGCGTGTTTCTAACCTCTTGTCAAGTTTTGATACTCTATTGCAGCAGGAGATGGACCACTTAAAGATGGCAGTAGCAAAGGTCGATGCACACCGCCCTGATGTCCTTCTGGTAGAGAAATCAGTTTCTCGATATGCCCAGGAATATCTTCTTGCAAAAGACATATCACTTGTTCTGAATATCAAGAGGCCACTTTTAGAGCGTATAGCTCGCTGCACAGGTGCTCAAATAGTCCCTTCAATTGATCATCTCTCATCACAGAAGTTGGGCTACTGCGACTTATTTCATGTGGAGAGGTTCATGGAAGATCTTGGTTCTGCCAGTCAGGGTGGGAAAAAACTGGTGAAGACATTGATGTATTTTGAAGGTTGCCCAAAGCCATTGGGTTGTACTATTCTACTTAGAGGTGCTAATGGGGATGAGTTAAAAAAATTGAAGCATGTGGTTCAGTATGGGATTTTTGCAGCATATCATTTGGCTTTGGAGACATCCTTCCTTGCCGATGAAGGAGCCACTCTGCCAGAACTCCCATTAAACACTCCAATTACTGTGGCGCTTCCAGATAAACCATCAAGCATTGAGAGGTCCATTTCCACGGTGCCTGGTTTTAGTGTTGCTGGCAATGGACAGTCTCACAATGAACCACGAAGATCCATCAGTGTCCCAGTTTCAGATTTGGACGCAGCAATCAGAAGTATACAACCTCCTTTACTAAGTGGTCATACCTCTCTACCCGCTCCTCCCACTACAGGTTTCACCAAGTCTACATCTGTGTATCCTACTCCGTCCGGGAATGCTTCAGATACCACATCTGTGTACTCTACTCCATCTGGGAATTCTTCAGATACTTATCATAAGGGTCTTTCTCCTTATCATATATTTGATGATAGAAATGAAATGGGTTCTAAAAAATTTTCACAGGTAGAAAATTCTGCAACCAAAATTTGCTCTGATATCATGTCTAATCATCTTGCTGGCAATAGCTTGAGGTATTTAGAGTCTATGGGACAGGGTGGCTTCTCGATCGCCCAAAATGATGAAACTGTAAGTACTGGAAATCAGCTAGGTGGTTCATACAATTCATTTCTGCATGAAGATGGTAATACTCAAGCTGATGAACCAGGACCTATGAATGAAGAGTTTCCACCGTCACCTTCTGACCATCAGAGTATTTTGGTTTCCTTGTCATCCCGGTGCGTGTGGAAGGGGACTGTCTGTGAGAGGTCGCATCTGTTTCGAATCAAATACTATGGCAGTTTTGATAAACCTTTAGGACGGTTTTTACAAGACCATTTATTTGATCAGAGTTATCAATGCAATTCTTGTGAGATGCCGTCAGAAGCACATGTTCATTGTTATACTCATCGTCAGGGTACTCTAACTATATCTGTTAAGAAGCTGCCAGAAATAATCTTACCAGGTGAAAAGGAAGGAAAGATCTGGATGTGGCACAGGTGCTTGAAGTGTCCAAGGATCAATGGATTTCCTCCGGCTACTAGGAGAATAGTGATGTCTGATGCCGCATGGGGTTTATCGTTTGGAAAATTTTTGGAGCTCAGTTTTTCAAACCATGCGGCTGCAAGCAGGGTGGCGAGCTGTGGCCATTCTTTACATAGAGATTGTCTTCGTTTTTATGGATTCGGGAAGATGGTCGCTTGCTTTCGGTATGCATCAATTTATGTTCATTCTGTCTACCTTCCACCCGCCAAAGTGGATTTCAATTATGAGAAGCAGGAATGGATACAAAAAGAAACAGATGAGGTTATTGATCGGGCAGAGCTTCTTTTTTCTGAAGTACTTAATGCTCTTCGTCAAATTGCGGAGAAAAGATCTGGTTCGGGATCACATAGTAGTGGCATGGTAACACCTGAATCAAGACACCAAATTGTCGAACTGGAAGGAATGTTACAAAAGGAGAAGGTGGAATTTGAGGAATTGCTCCAGAAAACTTTGAACAGGGAAGCTAAAAAGGGCCAGCCTGTCATTGACATTCTGGAGATCAATCGGCTGCGAAGGCAGCTGCTTTTCCAATCTTACATGTGGGACCATCGCTTAGTTTATGCAGCCAGTTTAGAGAACAACAGATACAACGATGGTTTGAATAGCTCAATTCCAGATGAGGGGAAACCTGCGACTAGTGGTGAAAAGATTGTTGATAATGTGGCAATAAATCTAGGAAAAAGTTATAATAGTTGTGATTCCTTTCTTGTGGATGCAATGCTTAACAAAGGATTTGACCACGGGGGAGACGTTGCCAACACTGTCCATGCTGAAATGCTAAATAAAGAGAGAGACAGCGTCCGAGATTCAAAATATGAAAATGAAGATCAAGGTAATCTCTCCGATGGAGTTAGTACCTATGATCAATCTGATCCTTTGAAACCAAGGGCAGGTATTCATAGGTCTCTCTCGGATGGTCAGTTTCCTGTCATAATGGATTTGTCAGATACCCTTGACACTGCGTGGACCGGTGAAAATCAATGTGGATTTGGAACAGCGAAGGACAATACCTGCACAGTTCCTGTTTTAGGAATAGCAGATTCAAGTGCCTCTCCTGTGAAGGACAAATTAAATTTAGACCATGCAGAAGACCAGAATGCATCCATGATTGCGCATGCCGCTTCTCCTGCATTATCTACCAAGGGGTCTGAAAATATCGAAGACTCTGTAAGCTGGTTGAAAATGCCCTTTTTAAACTTCTATCGTGGGTTCAATAAGAATTTTTTATCTGCTGCTCAGAAGCTTGATACACTCGGCGAGTATAATCCAGTATACGTTTCATCCTTTCGGGAATTGGAACTGGAAGGTGGGGCTAGGCTGCTTCTGCCGGTGGGGATAAACGATACTGTTGTCCCCGTATACGATGACGAGCCCACAAGTCTTATATCTTATGCCCTTATGTCACCAGAATATCATTCGCAAATAAGTGATGAGGGGGAAAGAACAAGAAACAGCGGTTATGTTGGTTTGTCTGATTCTTTCACTACGCAGTCACACCATCCTGATGATGACACAACATCTGAATCTCATCGAAGCTTTGGATCTACTGAGGAGAGCCTTTTGTCCACATATGGATCTCGTAGCTCCCTGGGTTTGGATCCGCTTTCATATACCAAGGCTTTGCATGCAAAAGTTTCTTTCGGAGATGATAGCCCACTTGGTCAGGTTAAATACTCAGTGACATGTTACCATGCAAAACGTTTTGAAGCCTTAAGAAGGATTTGCTGCCCGTCTGAGCTTGACTATGTAAGATCCCTTAGTCGTTGTAAAAAGTGGGGAGCCCAAGGTGGAAAGAGCAATGTCTTTTTTGCTAAAAGCTTGGATGACCGGTTTATCATTAAACAAGTCACCAAGACGGAGTTGGAATCTTTCATAAAATTCGCTCCCGGCTATTTCAAATATATGTCTGAATCAATTGGCACAAAAAGTCCGACATGCCTGGCTAAGATTTTGGGGATCTATCAGGTTAAATCAAAGCATCTTAAAGGAGGGAAAGAGTCGAAGATAGATGTCTTAGTTATGGAGAATCTTATGTTTGGCAGAAATATTACGCGGCTCTATGATCTCAAAGGATCTTCACGGTCTCGTTACAATCCTGATTCTAGTGGGAGTGACAAAGTGCTGCTAGATCAGAACTTGATTGAAGCGATGCCCACATCTCCGATCTTTGTGGGAAACAAGGCAAAGCGGTTGTTGGAGAGAGCTGTCTGGAATGACACTGCTTTTCTTGCTTCAATTGACGTAATGGATTATTCACTACTGGTTGGGGTGGACGAAGAGAAGCATGAATTAGTACTTGGAATTATTGATTTCATGAGGCAGTATACATGGGATAAGCACCTTGAAACTTGGGTTAAAGCTTCGGGCATCCTCGGTGGGCCAAAGAATGCTTCTCCTACTGTAATTTCCCCAAAGCAGTACAAGAAAAGGTTCCGGAAAGCAATGACAACCTATTTTCTGATGGTTCCAGATCAGTGGTCTCCTCCGTCGATTGTTACAAGTACCTCTCAGTCTGATTTGGGCGAAGAGACAGGTGGTAATTCGGTTGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000285 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005768 GO:0005773 GO:0005774 GO:0005942 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006810 GO:0006996 GO:0007033 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009555 GO:0009987 GO:0010008 GO:0010118 GO:0010256 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016301 GO:0016307 GO:0016310 GO:0016482 GO:0016740 GO:0016772 GO:0016773 GO:0019637 GO:0019898 GO:0030258 GO:0031090 GO:0031410 GO:0031982 GO:0032266 GO:0032501 GO:0032502 GO:0032991 GO:0035032 GO:0035091 GO:0042147 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0048229 GO:0048856 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061695 GO:0070772 GO:0071704 GO:0071840 GO:0090332 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:1901981 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00562 ko04070 ko04145 ko04810 map00562 map04070 map04145 map04810

Pfam Domains

Protein Families

Cpn60_TCP1 FYVE PIP5K

Protein Analysis

1835

Amino Acids

203.74

Weight (kDa)

5.5

Isoelectric Point (pI)

45.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FYVE	PF01363	33 - 101	7.8e-18	FYVE zinc finger
Cpn60_TCP1	PF00118	407 - 651	1.6e-30	TCP-1/cpn60 chaperonin family
PIP5K	PF01504	1579 - 1745	3e-35	Phosphatidylinositol-4-phosphate 5-Kinase
PIP5K	PF01504	1744 - 1801	7.2e-08	Phosphatidylinositol-4-phosphate 5-Kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005238)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G14270 AT3G14270
fragaria_vesca	FvH4_6g35770 FvH4_6g35770 FvH4_6g35770
malus_domestica	MD09G1173300.v1.1 MD17G1154000.v1.1
prunus_persica	Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1
pyrus_communis	pycom09g09070 pycom17g14660
rosa_chinensis	RchiOBHm_Chr2g0147811
rosa_laevigata	RLG00000020280
rosa_multiflora	Rmu_sc0000308.1_g000046
rosa_roxburghii	Rroxscaffold_2G00099330
rosa_rugosa	Rorug02G0401800 Rorug02G0401800 Rorug02G0401800 Rorug02G0401900
rosa_samantha	Rh2AG459000 Rh2BG472300 Rh2CG446400 Rh2DG481300
rosa_wichuraiana	Rw2G037540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3639
Acc16I	TGCGCA	1 cut(s)	4065
Acc36I	ACCTGC	6 cut(s)	232, 796, 1159, 3589, 3833, 3971
AccB1I	GGYRCC	1 cut(s)	2050
AccBSI	CCGCTC	1 cut(s)	2189
AccI	GTMKAC	7 cut(s)	175, 2149, 2216, 3144, 4224, 4311, 5276
AccII	CGCG	1 cut(s)	5005
AccIII	TCCGGA	1 cut(s)	5385
AclI	AACGTT	1 cut(s)	4651
AcoI	YGGCCR	1 cut(s)	3052
AcuI	CTGAAG	3 cut(s)	2235, 2277, 3264
AdeI	CACNNNGTG	1 cut(s)	3915
AfiI	CCNNNNNNNGG	7 cut(s)	329, 2201, 3572, 3842, 3980, 4546, 4668
AflII	CTTAAG	1 cut(s)	4663
AflIII	ACRYGT	3 cut(s)	2797, 3489, 4634
AgeI	ACCGGT	2 cut(s)	3920, 4779
AhlI	ACTAGT	1 cut(s)	3587
AjnI	CCWGG	9 cut(s)	605, 1238, 1294, 1590, 2053, 2572, 2869, 4539, 4890
AjuI	GAANNNNNNNTTGG	6 cut(s)	3152, 3184, 5232, 5264, 5326, 5358
AleI	CACNNNNGTG	1 cut(s)	2264
Alw21I	GWGCWC	4 cut(s)	1107, 1444, 1678, 3009
AlwNI	CAGNNNCTG	4 cut(s)	74, 492, 917, 3974
Aor13HI	TCCGGA	1 cut(s)	5385
AoxI	GGCC	7 cut(s)	452, 481, 1113, 1640, 3052, 3424, 5330
ArsI	GACNNNNNNTTYG	2 cut(s)	2732, 2764
AsiGI	ACCGGT	2 cut(s)	3920, 4779
Asp700I	GAANNNNTTC	4 cut(s)	990, 1599, 2592, 5381
AspLEI	GCGC	3 cut(s)	10, 2008, 4066
AspS9I	GGNCC	9 cut(s)	452, 1291, 1507, 2037, 2576, 3424, 3496, 3918, 5330
AsuC2I	CCSGG	3 cut(s)	2242, 2644, 4839
AsuII	TTCGAA	1 cut(s)	2689
AsuNHI	GCTAGC	1 cut(s)	386
AvaII	GGWCC	6 cut(s)	1291, 1507, 2037, 2576, 3496, 3918
BaeI	ACNNNNGTAYC	2 cut(s)	4287, 4320
BalI	TGGCCA	1 cut(s)	3054
BamHI	GGATCC	2 cut(s)	54, 4549
BanI	GGYRCC	1 cut(s)	2050
BanII	GRGCYC	3 cut(s)	3009, 4326, 4735
BarI	GAAGNNNNNNTAC	4 cut(s)	1706, 1738, 3342, 3374
BbsI	GAAGAC	4 cut(s)	1805, 3068, 4047, 4119
Bbv12I	GWGCWC	4 cut(s)	1107, 1444, 1678, 3009
BbvCI	CCTCAGC	1 cut(s)	485
BceAI	ACGGC	1 cut(s)	2770
BcgI	CGANNNNNNTGC	2 cut(s)	2669, 2703
BciT130I	CCWGG	9 cut(s)	607, 1240, 1296, 1592, 2055, 2574, 2871, 4541, 4892
BciVI	GTATCC	2 cut(s)	2238, 3186
BclI	TGATCA	4 cut(s)	106, 1696, 2752, 3814
BcnI	CCSGG	3 cut(s)	2242, 2644, 4839
BcuI	ACTAGT	1 cut(s)	3587
BfaI	CTAG	8 cut(s)	387, 2516, 2949, 3588, 3626, 4266, 5055, 5078
BfmI	CTRYAG	4 cut(s)	115, 894, 1023, 2199
BfoI	RGCGCY	1 cut(s)	2009
BfrI	CTTAAG	1 cut(s)	4663
BfuAI	ACCTGC	6 cut(s)	232, 796, 1159, 3589, 3833, 3971
BfuI	GTATCC	2 cut(s)	2238, 3186
BglII	AGATCT	4 cut(s)	352, 1759, 2889, 3281
BlpI	GCTNAGC	1 cut(s)	1158
BmcAI	AGTACT	4 cut(s)	361, 2503, 3249, 5247
Bme18I	GGWCC	6 cut(s)	1291, 1507, 2037, 2576, 3496, 3918
BmgT120I	GGNCC	9 cut(s)	452, 1291, 1507, 2037, 2576, 3424, 3496, 3918, 5330
BmiI	GGNNCC	9 cut(s)	56, 1293, 1963, 2052, 3497, 4551, 4732, 5384, 5418
BmrI	ACTGGG	4 cut(s)	459, 786, 867, 2111
BmtI	GCTAGC	1 cut(s)	390
BmuI	ACTGGG	4 cut(s)	459, 786, 867, 2111
BpiI	GAAGAC	4 cut(s)	1805, 3068, 4047, 4119
BpmI	CTGGAG	3 cut(s)	564, 3377, 3467
Bpu10I	CCTNAGC	1 cut(s)	485
Bpu1102I	GCTNAGC	1 cut(s)	1158
Bpu14I	TTCGAA	1 cut(s)	2689
BpuEI	CTTGAG	4 cut(s)	651, 1466, 2458, 2544
BpuMI	CCSGG	3 cut(s)	2242, 2644, 4839
BsaAI	YACGTR	1 cut(s)	1046
BsaBI	GATNNNNATC	1 cut(s)	2301
BsaI	GGTCTC	3 cut(s)	3861, 5040, 5436
BsaWI	WCCGGW	3 cut(s)	3920, 4779, 5385
Bsc4I	CCNNNNNNNGG	7 cut(s)	329, 2201, 3572, 3842, 3980, 4546, 4668
Bse118I	RCCGGY	3 cut(s)	3920, 4279, 4779
Bse3DI	GCAATG	5 cut(s)	718, 2080, 3675, 4756, 5400
Bse8I	GATNNNNATC	1 cut(s)	2301
BseAI	TCCGGA	1 cut(s)	5385
BseBI	CCWGG	9 cut(s)	607, 1240, 1296, 1592, 2055, 2574, 2871, 4541, 4892
BseJI	GATNNNNATC	1 cut(s)	2301
BseLI	CCNNNNNNNGG	7 cut(s)	329, 2201, 3572, 3842, 3980, 4546, 4668
BseMI	GCAATG	5 cut(s)	718, 2080, 3675, 4756, 5400
BseMII	CTCAG	8 cut(s)	499, 1172, 3022, 4202, 4491, 4641, 4677, 5474
BseRI	GAGGAG	5 cut(s)	769, 1115, 2181, 4517, 5424
BseYI	CCCAGC	1 cut(s)	11
BsgI	GTGCAG	2 cut(s)	1651, 3949
Bsh1236I	CGCG	1 cut(s)	5005
Bsh1285I	CGRYCG	1 cut(s)	2478
BshFI	GGCC	7 cut(s)	454, 483, 1115, 1642, 3054, 3426, 5332
BshNI	GGYRCC	1 cut(s)	2050
BshTI	ACCGGT	2 cut(s)	3920, 4779
BsiEI	CGRYCG	1 cut(s)	2478
BsiHKAI	GWGCWC	4 cut(s)	1107, 1444, 1678, 3009
BsiSI	CCGG	8 cut(s)	2241, 2644, 2942, 3921, 4280, 4780, 4839, 5386
BslFI	GGGAC	6 cut(s)	1670, 2099, 2472, 2674, 3509, 4289
BslI	CCNNNNNNNGG	7 cut(s)	329, 2201, 3572, 3842, 3980, 4546, 4668
BsmBI	CGTCTC	3 cut(s)	507, 1236, 3693
BsmFI	GGGAC	6 cut(s)	1670, 2099, 2472, 2674, 3509, 4289
BsmI	GAATGC	3 cut(s)	2251, 4054, 5344
BsnI	GGCC	7 cut(s)	454, 483, 1115, 1642, 3054, 3426, 5332
Bso31I	GGTCTC	3 cut(s)	3861, 5040, 5436
Bsp119I	TTCGAA	1 cut(s)	2689
Bsp1286I	GDGCHC	6 cut(s)	1107, 1444, 1678, 3009, 4326, 4735
Bsp13I	TCCGGA	1 cut(s)	5385
Bsp1407I	TGTACA	1 cut(s)	206
Bsp1720I	GCTNAGC	1 cut(s)	1158
Bsp19I	CCATGG	1 cut(s)	39
BspANI	GGCC	7 cut(s)	454, 483, 1115, 1642, 3054, 3426, 5332
BspCNI	CTCAG	8 cut(s)	498, 1171, 3021, 4201, 4492, 4640, 4678, 5473
BspEI	TCCGGA	1 cut(s)	5385
BspFNI	CGCG	1 cut(s)	5005
BspHI	TCATGA	2 cut(s)	796, 5265
BspLI	GGNNCC	9 cut(s)	56, 1293, 1963, 2052, 3497, 4551, 4732, 5384, 5418
BspMAI	CTGCAG	1 cut(s)	898
BspMI	ACCTGC	6 cut(s)	232, 796, 1159, 3589, 3833, 3971
BspOI	GCTAGC	1 cut(s)	390
BspQI	GCTCTTC	1 cut(s)	3264
BspT104I	TTCGAA	1 cut(s)	2689
BspT107I	GGYRCC	1 cut(s)	2050
BspTI	CTTAAG	1 cut(s)	4663
BspTNI	GGTCTC	3 cut(s)	3861, 5040, 5436
BsrBI	CCGCTC	1 cut(s)	2189
BsrDI	GCAATG	5 cut(s)	718, 2080, 3675, 4756, 5400
BsrFI	RCCGGY	3 cut(s)	3920, 4279, 4779
BsrGI	TGTACA	1 cut(s)	206
BssAI	RCCGGY	3 cut(s)	3920, 4279, 4779
BssNAI	GTATAC	4 cut(s)	2150, 4225, 4312, 5277
BssT1I	CCWWGG	9 cut(s)	39, 59, 242, 1432, 2921, 3836, 4092, 4566, 4735
Bst1107I	GTATAC	4 cut(s)	2150, 4225, 4312, 5277
Bst2UI	CCWGG	9 cut(s)	607, 1240, 1296, 1592, 2055, 2574, 2871, 4541, 4892
Bst6I	CTCTTC	4 cut(s)	2583, 3264, 5223, 5472
BstAFI	CTTAAG	1 cut(s)	4663
BstAPI	GCANNNNNTGC	1 cut(s)	733
BstAUI	TGTACA	1 cut(s)	206
BstBAI	YACGTR	1 cut(s)	1046
BstBI	TTCGAA	1 cut(s)	2689
BstDSI	CCRYGG	3 cut(s)	39, 2046, 3691
BstEII	GGTNACC	1 cut(s)	236
BstENI	CCTNNNNNAGG	2 cut(s)	3978, 4666
BstFNI	CGCG	1 cut(s)	5005
BstH2I	RGCGCY	1 cut(s)	2009
BstHHI	GCGC	3 cut(s)	10, 2008, 4066
BstMCI	CGRYCG	1 cut(s)	2478
BstNI	CCWGG	9 cut(s)	607, 1240, 1296, 1592, 2055, 2574, 2871, 4541, 4892
BstNSI	RCATGY	7 cut(s)	1895, 2801, 3493, 4070, 4580, 4638, 4890
BstPI	GGTNACC	1 cut(s)	236
BstSFI	CTRYAG	4 cut(s)	115, 894, 1023, 2199
BstUI	CGCG	1 cut(s)	5005
BstV2I	GAAGAC	4 cut(s)	1805, 3068, 4047, 4119
BstXI	CCANNNNNNTGG	1 cut(s)	5427
BstZ17I	GTATAC	4 cut(s)	2150, 4225, 4312, 5277
BsuI	GTATCC	2 cut(s)	2238, 3186
BsuRI	GGCC	7 cut(s)	454, 483, 1115, 1642, 3054, 3426, 5332
BtgI	CCRYGG	3 cut(s)	39, 2046, 3691
BtgZI	GCGATG	2 cut(s)	3485, 5114
BtsI	GCAGTG	2 cut(s)	3909, 5169
BtsIMutI	CAGTG	9 cut(s)	405, 562, 679, 2116, 3711, 3909, 4635, 5169, 5432
BveI	ACCTGC	6 cut(s)	232, 796, 1159, 3589, 3833, 3971
CaiI	CAGNNNCTG	4 cut(s)	74, 492, 917, 3974
CciI	TCATGA	2 cut(s)	796, 5265
CfoI	GCGC	3 cut(s)	10, 2008, 4066
Cfr10I	RCCGGY	3 cut(s)	3920, 4279, 4779
Cfr13I	GGNCC	9 cut(s)	452, 1291, 1507, 2037, 2576, 3424, 3496, 3918, 5330
CseI	GACGC	3 cut(s)	1028, 2141, 3739
CsiI	ACCWGGT	1 cut(s)	2869
CspAI	ACCGGT	2 cut(s)	3920, 4779
CspCI	CAANNNNNGTGG	4 cut(s)	1983, 2018, 3021, 3056
DraI	TTTAAA	1 cut(s)	4146
DraIII	CACNNNGTG	1 cut(s)	3915
EaeI	YGGCCR	1 cut(s)	3052
Eam1104I	CTCTTC	4 cut(s)	2583, 3264, 5223, 5472
EarI	CTCTTC	4 cut(s)	2583, 3264, 5223, 5472
Ecl136II	GAGCTC	1 cut(s)	3007
Eco130I	CCWWGG	9 cut(s)	39, 59, 242, 1432, 2921, 3836, 4092, 4566, 4735
Eco24I	GRGCYC	3 cut(s)	3009, 4326, 4735
Eco31I	GGTCTC	3 cut(s)	3861, 5040, 5436
Eco32I	GATATC	1 cut(s)	2407
Eco47I	GGWCC	6 cut(s)	1291, 1507, 2037, 2576, 3496, 3918
Eco53kI	GAGCTC	1 cut(s)	3007
Eco57I	CTGAAG	3 cut(s)	2235, 2277, 3264
Eco91I	GGTNACC	1 cut(s)	236
EcoICRI	GAGCTC	1 cut(s)	3007
EcoNI	CCTNNNNNAGG	2 cut(s)	3978, 4666
EcoO109I	RGGNCCY	1 cut(s)	2576
EcoO65I	GGTNACC	1 cut(s)	236
EcoRI	GAATTC	1 cut(s)	2287
EcoRII	CCWGG	9 cut(s)	605, 1238, 1294, 1590, 2053, 2572, 2869, 4539, 4890
EcoRV	GATATC	1 cut(s)	2407
EcoT14I	CCWWGG	9 cut(s)	39, 59, 242, 1432, 2921, 3836, 4092, 4566, 4735
EcoT22I	ATGCAT	2 cut(s)	3124, 4054
EcoT38I	GRGCYC	3 cut(s)	3009, 4326, 4735
ErhI	CCWWGG	9 cut(s)	39, 59, 242, 1432, 2921, 3836, 4092, 4566, 4735
Esp3I	CGTCTC	3 cut(s)	507, 1236, 3693
FaqI	GGGAC	6 cut(s)	1670, 2099, 2472, 2674, 3509, 4289
FauI	CCCGC	2 cut(s)	2194, 3163
FauNDI	CATATG	1 cut(s)	4522
FbaI	TGATCA	4 cut(s)	106, 1696, 2752, 3814
FblI	GTMKAC	7 cut(s)	175, 2149, 2216, 3144, 4224, 4311, 5276
FriOI	GRGCYC	3 cut(s)	3009, 4326, 4735
FspBI	CTAG	8 cut(s)	387, 2516, 2949, 3588, 3626, 4266, 5055, 5078
FspI	TGCGCA	1 cut(s)	4065
GlaI	GCGC	3 cut(s)	9, 2007, 4065
GsaI	CCCAGC	1 cut(s)	15
GsuI	CTGGAG	3 cut(s)	564, 3377, 3467
HaeII	RGCGCY	1 cut(s)	2009
HaeIII	GGCC	7 cut(s)	454, 483, 1115, 1642, 3054, 3426, 5332
HapII	CCGG	8 cut(s)	2241, 2644, 2942, 3921, 4280, 4780, 4839, 5386
HgaI	GACGC	3 cut(s)	1028, 2141, 3739
HhaI	GCGC	3 cut(s)	10, 2008, 4066
Hin6I	GCGC	3 cut(s)	8, 2006, 4064
HinP1I	GCGC	3 cut(s)	8, 2006, 4064
HincII	GTYRAC	1 cut(s)	176
HindII	GTYRAC	1 cut(s)	176
HindIII	AAGCTT	4 cut(s)	4193, 4486, 4768, 5310
HpaII	CCGG	8 cut(s)	2241, 2644, 2942, 3921, 4280, 4780, 4839, 5386
Hpy99I	CGWCG	2 cut(s)	1395, 5443
HpyCH4IV	ACGT	6 cut(s)	1045, 1229, 3702, 4227, 4651, 5193
HpySE526I	ACGT	6 cut(s)	1045, 1229, 3702, 4227, 4651, 5193
HspAI	GCGC	3 cut(s)	8, 2006, 4064
Kpn2I	TCCGGA	1 cut(s)	5385
Ksp22I	TGATCA	4 cut(s)	106, 1696, 2752, 3814
LguI	GCTCTTC	1 cut(s)	3264
MabI	ACCWGGT	1 cut(s)	2869
MaeI	CTAG	8 cut(s)	387, 2516, 2949, 3588, 3626, 4266, 5055, 5078
MaeII	ACGT	6 cut(s)	1045, 1229, 3702, 4227, 4651, 5193
MbiI	CCGCTC	1 cut(s)	2189
MfeI	CAATTG	3 cut(s)	1692, 4866, 5187
MhlI	GDGCHC	6 cut(s)	1107, 1444, 1678, 3009, 4326, 4735
MlsI	TGGCCA	1 cut(s)	3054
MluNI	TGGCCA	1 cut(s)	3054
MlyI	GAGTC	4 cut(s)	794, 2459, 4109, 4958
MmeI	TCCRAC	5 cut(s)	847, 1197, 4794, 4907, 5127
Mox20I	TGGCCA	1 cut(s)	3054
Mph1103I	ATGCAT	2 cut(s)	3124, 4054
MroI	TCCGGA	1 cut(s)	5385
MroXI	GAANNNNTTC	4 cut(s)	990, 1599, 2592, 5381
MscI	TGGCCA	1 cut(s)	3054
MslI	CAYNNNNRTG	7 cut(s)	741, 1044, 2264, 2547, 2644, 3303, 4455
Msp20I	TGGCCA	1 cut(s)	3054
MspA1I	CMGCKG	3 cut(s)	1162, 3472, 4407
MspCI	CTTAAG	1 cut(s)	4663
MspI	CCGG	8 cut(s)	2241, 2644, 2942, 3921, 4280, 4780, 4839, 5386
MunI	CAATTG	3 cut(s)	1692, 4866, 5187
Mva1269I	GAATGC	3 cut(s)	2251, 4054, 5344
MvaI	CCWGG	9 cut(s)	607, 1240, 1296, 1592, 2055, 2574, 2871, 4541, 4892
MvnI	CGCG	1 cut(s)	5005
NciI	CCSGG	3 cut(s)	2242, 2644, 4839
NcoI	CCATGG	1 cut(s)	39
NdeI	CATATG	1 cut(s)	4522
NheI	GCTAGC	1 cut(s)	386
NlaIV	GGNNCC	9 cut(s)	56, 1293, 1963, 2052, 3497, 4551, 4732, 5384, 5418
NmeAIII	GCCGAG	1 cut(s)	4185
NsbI	TGCGCA	1 cut(s)	4065
NsiI	ATGCAT	2 cut(s)	3124, 4054
NspI	RCATGY	7 cut(s)	1895, 2801, 3493, 4070, 4580, 4638, 4890
NspV	TTCGAA	1 cut(s)	2689
OliI	CACNNNNGTG	1 cut(s)	2264
PaeI	GCATGC	2 cut(s)	4070, 4580
PagI	TCATGA	2 cut(s)	796, 5265
PasI	CCCWGGG	1 cut(s)	4540
PciI	ACATGT	3 cut(s)	2797, 3489, 4634
PciSI	GCTCTTC	1 cut(s)	3264
PcsI	WCGNNNNNNNCGW	1 cut(s)	3699
PctI	GAATGC	3 cut(s)	2251, 4054, 5344
PdmI	GAANNNNTTC	4 cut(s)	990, 1599, 2592, 5381
PflFI	GACNNNGTC	1 cut(s)	3749
PfoI	TCCNGGA	1 cut(s)	2240
PinAI	ACCGGT	2 cut(s)	3920, 4779
Ple19I	CGATCG	1 cut(s)	2478
PleI	GAGTC	4 cut(s)	794, 2458, 4109, 4957
PpsI	GAGTC	4 cut(s)	794, 2458, 4109, 4957
Ppu21I	YACGTR	1 cut(s)	1046
PpuMI	RGGWCCY	1 cut(s)	2576
PscI	ACATGT	3 cut(s)	2797, 3489, 4634
PsiI	TTATAA	1 cut(s)	3639
Psp124BI	GAGCTC	1 cut(s)	3009
Psp1406I	AACGTT	1 cut(s)	4651
Psp5II	RGGWCCY	1 cut(s)	2576
Psp6I	CCWGG	9 cut(s)	605, 1238, 1294, 1590, 2053, 2572, 2869, 4539, 4890
PspEI	GGTNACC	1 cut(s)	236
PspFI	CCCAGC	1 cut(s)	11
PspGI	CCWGG	9 cut(s)	605, 1238, 1294, 1590, 2053, 2572, 2869, 4539, 4890
PspN4I	GGNNCC	9 cut(s)	56, 1293, 1963, 2052, 3497, 4551, 4732, 5384, 5418
PspPI	GGNCC	9 cut(s)	452, 1291, 1507, 2037, 2576, 3424, 3496, 3918, 5330
PspPPI	RGGWCCY	1 cut(s)	2576
PsrI	GAACNNNNNNTAC	2 cut(s)	3521, 3553
PstI	CTGCAG	1 cut(s)	898
PstNI	CAGNNNCTG	4 cut(s)	74, 492, 917, 3974
PsyI	GACNNNGTC	1 cut(s)	3749
PvuI	CGATCG	1 cut(s)	2478
PvuII	CAGCTG	2 cut(s)	1162, 3472
RseI	CAYNNNNRTG	7 cut(s)	741, 1044, 2264, 2547, 2644, 3303, 4455
SacI	GAGCTC	1 cut(s)	3009
SalI	GTCGAC	1 cut(s)	174
SapI	GCTCTTC	1 cut(s)	3264
Sau96I	GGNCC	9 cut(s)	452, 1291, 1507, 2037, 2576, 3424, 3496, 3918, 5330
ScaI	AGTACT	4 cut(s)	361, 2503, 3249, 5247
SchI	GAGTC	4 cut(s)	794, 2459, 4109, 4958
SduI	GDGCHC	6 cut(s)	1107, 1444, 1678, 3009, 4326, 4735
SexAI	ACCWGGT	1 cut(s)	2869
SfcI	CTRYAG	4 cut(s)	115, 894, 1023, 2199
SfuI	TTCGAA	1 cut(s)	2689
SinI	GGWCC	6 cut(s)	1291, 1507, 2037, 2576, 3496, 3918
SmiMI	CAYNNNNRTG	7 cut(s)	741, 1044, 2264, 2547, 2644, 3303, 4455
SmlI	CTYRAG	5 cut(s)	666, 1445, 2437, 2559, 4663
SmoI	CTYRAG	5 cut(s)	666, 1445, 2437, 2559, 4663
SpeI	ACTAGT	1 cut(s)	3587
SphI	GCATGC	2 cut(s)	4070, 4580
SspI	AATATT	2 cut(s)	754, 4999
SspMI	CTAG	8 cut(s)	387, 2516, 2949, 3588, 3626, 4266, 5055, 5078
SstI	GAGCTC	1 cut(s)	3009
StyI	CCWWGG	9 cut(s)	39, 59, 242, 1432, 2921, 3836, 4092, 4566, 4735
TaiI	ACGT	6 cut(s)	1048, 1232, 3705, 4230, 4654, 5196
TaqII	GACCGA	2 cut(s)	530, 1080
TatI	WGTACW	8 cut(s)	206, 359, 1841, 2268, 2501, 3247, 5245, 5370
TauI	GCSGC	4 cut(s)	2975, 3029, 4073, 5008
TscAI	CASTG	9 cut(s)	412, 562, 679, 2116, 3718, 3916, 4635, 5176, 5432
TspGWI	ACGGA	4 cut(s)	345, 2226, 4823, 5427
TspRI	CASTG	9 cut(s)	412, 562, 679, 2116, 3718, 3916, 4635, 5176, 5432
Tth111I	GACNNNGTC	1 cut(s)	3749
Vha464I	CTTAAG	1 cut(s)	4663
VpaK11BI	GGWCC	6 cut(s)	1291, 1507, 2037, 2576, 3496, 3918
XagI	CCTNNNNNAGG	2 cut(s)	3978, 4666
XceI	RCATGY	7 cut(s)	1895, 2801, 3493, 4070, 4580, 4638, 4890
XcmI	CCANNNNNNNNNTGG	1 cut(s)	4810
XmiI	GTMKAC	7 cut(s)	175, 2149, 2216, 3144, 4224, 4311, 5276
XmnI	GAANNNNTTC	4 cut(s)	990, 1599, 2592, 5381
XspI	CTAG	8 cut(s)	387, 2516, 2949, 3588, 3626, 4266, 5055, 5078
ZrmI	AGTACT	4 cut(s)	361, 2503, 3249, 5247
Zsp2I	ATGCAT	2 cut(s)	3124, 4054

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.