Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0005238

Physical Location & Seq

Genomic Coordinates Reverse (-)

20926218 .. 20935393

Gene Structure 9176 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00099330.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5094 bp

ATGGCTGCCCCCAATAAGTTTTTTTCTCAGTTTATTTCCATGGTGAAATCATGGATCCCTTGGCGGTCTGAGCCGGCTAATGTTTCGAGGGATTTTTGGATGCCTGATGATAGCTGTAGGGTATGTTATGAGTGTGATTCACAGTTCACAGTATTTAACCGTAAACATCATTGTAGACTTTGTGGACGAGTTTTCTGTGCCAAGTGTACAGAAAACTCAATTCCTGCCCCCTCTGGTGACTCAAGGATAGATCGGGAAGAGAGGGAAAGGATTCGTGTCTGCAATTTTTGTTACAAGCAACATCAGCAAGGCATAGCTGCTACTCCTGATAATGGAACACAGATTGCTAATCTAGATCTTAGTACTTCACCATCAGAAACAAGTTTTACTAGCTTTAAGTCATGCGGCACTGGTAACAGCAGTAGCTTTACTCTTAACTCAATCCCGTACTCAACTGGGCCATACCAACGGCTTCAAAACAGTTCTGGTCTTAGCCCCTGTCAATCATCTCCCATGAAAACAAGCTCAGAAAAGCACAGCAAATATGGCTCCTGGAGGACCAATGATTTTGTTGCAGATATAGGGGATTCATCTCCAAATCATTATGAGATTTCAACAACCAGGAGTGATGACGACGATGTTGACTATGGGACATATCAGTCAGATTCTAAGAATTATTCTCAAGTCAATGATTACTATGGTCATGTTGAGTTTTATGAGATGAGCAATCATGATGAATCACATAAGGTGCCACTTGATGGTGAAAACATTGATGCAAAGCATTTAAGCAGCTCCCCGTTACTTCACAGTTTTAACTCGCAGGGTTCGGATCCACCACTTGAAAAGAAAGAAGATGAACATGATATGGAACAATGCCCTTCTTTGGCTTCCCCTGAACCAGAAGATGAAGAAGATGAAAGGGAAACTGTTCTTCTTGATGATGACGATGATGGTGATGCTGCAGGAGAGTGGGGAACCTTACGCACATCAAGCAGTTTTGGCAGCGGGGAGTCTCGCAATAGGGATCGATCAGGTGAGGAGCATAAGAAGGTGATGAAGAATGTGGTTGATGGGCACTTTAGGGCATTGGTAGCTCAGCTATTGCAGGTTGAGAACCTCCCTGTAGGCCAGGAAGGTGAAAATGAAAGTTGGTTGGAGATCATTACGTATCTGTCTTGGGAGGCTGCTACACTGTTAAAGCCAGATATGAGCAAAGGCGGAGGGATGGATCCTGGTGGTTATGTAAAAGTAAAATGCATAGCTTCTGGACGTCCCTCTGACAGTATGGTGGTCAAAGGAGTTGTCTGTAAGAAAAATGTGGCTCATCGACGAATGGCATCTAAAATGGAGAAACCTCGGTTCATGATTCTTGGTGGTGCTCTTGAGTACCAGCGAGTTTCTAACCTATTGTCAAGTTTTGATACTCTTTTACAGCAGGAGATGGACCATTTAAAGATGGCAGTAGCAAAGATTGAAGCACACCACCCTGATGTCCTTCTGGTGGAGAAATCAGTTTCTCGACATGCTCAGGAATATCTTCTTGCAAAAGACATATCACTTGTTCTTAATGTCAAGAGGCCACTTTTAGAGCGCATAGCTCGCTGCACAGGTGCTCAAATAGTTCCATCTATCGATCATCTCTCTTCACAAAAGTTGGGCTACTGTGAGACATTTCATGTTGGGAGGTTTCTGGAAGATCTTGGTTCTGCTGGCCAGGGTGGGAAGAAATTGATTTTACTCAGAGGTGCCAATGGGGATGAGTTGAAGAAAGTGAAGCATGTGGTTCAGTATGGGGTTTTTGCAGCATATCACTTGGCTTTGGAGACATCCTTCCTTGCTGATGAAGGTGCCTCTCTGCCTGAACTCCCATTCCAGTCTCCAATTACTGTGGCACTTCCAGATAAACCATCGAGTATTGAGAGGTCCATCTCAACAGTACCTGGTTTTAGAATTGGTGCCAACGAAAAGTCTCTGGGAGCTCAACCTCAGAGTGAACCACGAAGAGCCAACAGTGTCCCGATTACAGATTTTGACTCAGCTATTAAGAGTAGACCACCATGTTTACTAACTGGTCGTAGCTCTCTACCTGTTCGTCCAACTTCAAGTTCCACAAATTCTACCGGTTTACACTCTGCCCCACCTGGGAACGGTGTTACTTTTCATATCGCTGATAATCAAAATGAAATGGGCTCAAAAGATTCTTGGGTGGTGGAAACTTCTGCAACCAAACCTAGCTCTGATAATATGTCTAATCATCTTACTGCAAATAGCATGGGATCTTCAGAGGCCCTGGGACAAGGTATCTTCTCCAATACCCAAAATGATCCAAGTGTAAATCAGCTAGGTAGTTCAGACAATCCAATGCTGCATCAAGATGGTCAAACCCATGCTGCAGATGCAGGAACCATGAATGAAGAGTTCCCTCCGTCACCTGCTGACCATCAGAGCATTTTAGTTTCCTTGTCATCTCGATGCGTGTGGAAGGGGACTGTCTGTGAGAGGTCACATCTGTTCCGAATTAAATACTACGGAAGTTTTGATAAGCCTTTAGGACGGTTTTTGCGAGACCATTTATTTGATCAGACCTATCAATGCCATTCTTGCGAGATGCCATCAGAGGCACATGTTCATTGTTATACTCACCGACAGGGTACACTCACTATATCTGTTAAGAGGCTGCCAGAAATTTTCTTACCAGGTGAAAGGGAAGGGAAGATCTGGATGTGGCACAGATGTTTGAGGTGTCCAAGGATCAGTGGATTTCCTCCAGCTACTCGGAGAATAGTGATGTCTGATGCTGCATGGGGTTTGTCATTTGGGAAGTTTTTGGAGCTCAGTTTTTCAAACCACGCGGCTGCAAGCAGGGTGGCGAGCTGTGGCCATTCCTTACATAGAGATTGTCTTCGTTTCTATGGATTTGGGAAAATGGTTGCTTGCTTTCGGTATGCATCGATTGATGTTCATTCTGTCTACCTTCCACCCTCCAAACTGGATTTCATTTCCGAGAAGCAGGAGTGGATACAAAAAGAAACTAATGAGGTTGTTGCCCGAGCAGAGCTTCTTTTCTCTGAAGTACTTAATGCTCTTAGTCAAATTTCGGAGAAAAGATCTGGATCAGGGTCAATTACTAGTGGCATTGTAACACCTGAATCCAGACACCAAATAGTGGAACTGGAAGGAATGTTACAGAAGGAGAAGGTGGAATTTGAGGAGTTACTTCAGAAAACTTTGAAGAGAGAACCTAAGAAGGGCCAACCTGTTATCGACATTCTGGAGATCAATCGATTGCGGAGGCAGTTGTTTTTTCAATCTTATATGTGGGACCACCGCTTGGTTTATGCAGCCAGTTTAGATAACAACAGCTTCCAGGATAGTTTGAGCGGCTCAATTCCAGCTGAGGATAAACCCGTGGCTACTAACGAAAAGCTTGCTGAGATGGATGTAGAAATAAAGCCAGGAAAAGGCTATAATAGTTGTGATTCCTTTCTGGTGGATGCAATGCTTAGTGACGGCTTTGACCAGGAGGGAGGCTTTACTAGCGCTGCTATCAATGCAGACATGGTTCATGGAGAGGACATGAGTGATGATTTGAATAAGGAAAAAGGTCAAGCTAATCTCCCTACTAGCACAAGTGTCGGTGCTCAATCTGATCCTTTGACACCAAGGACAGCTCTTCGCAGGGTCCTATCTGATGGTCAGCATCCTCTCATGCTGAATTTGTCAGATACCCTTGACACTGCATGGACCGGTGAAAATCTCATTGGAGTTGTGGTAGCAAAGGAGAACTCGTGTCCAGTTCCTGACTTGGCCGTAGAAAATTCTTCAAATGCCTCTCCGGCAGAGGGATTAAATTTAGACCATGCAGAAGCCCAAAATGGGACCAAAGTTGCCCATTATGTGTCACCTGCATTGTCGACCAAGGGGTCTGAGAATATGGAAGACACAGTAAGCTGGTTAAAAATGCCCTTTTTGAACTTCTACCGTTCACTAAATAAGAATTTTTTATCTGCTGCTCAGAAGTTTGATACACTTGGTGGGTACAATCCAGTCTATGTGTCATCCTTTCGGGAGTTGGAACTGGAAGGTGGTGCTAGGCTGCTCTTGCCTGTGGGTGAAAACGATACTGTTGTCCCTGTATATGATGATGAGCCCACAAGTCTTATAGCCTATGCCCTTGTTTCATCAGATTATCAGTTACAAACCAGTGATGAGGCGGAAAGAGCAAAAGACAGTGGGGATATTATGGCCGCAGTATCTTTTACTGATTCAGTCATTATGCATCCAGATGATGACACTGTGTCTGAAACTCATAGAAGTCTTGGGTCTACTGAGGAGAGCATACTATCAATGTCTGGATCTCGTGGCTCCCTGGTCAAACAAGTCACCAAGACAGAGCTGGAATCTTTCATTAAATTTGCACCTGCATATTTCAAGTACCTCTCTGAGTCAATTAGCACAGGAAGTCCAACCTGCCTGGCAAAAATTCTGGGGATATATCAGGTTATGTCGAAGCATGTTAAGGGAGGCAAAGAAACAAAGATGGATGTTTTGATTATGGAGAATCTTCTGTTTGGAAGGACTGTAACACGACTCTATGATCTCAAAGGATCTTCGCGGTCAAGGTATAATCCTGATTCTAGTGGGAGTAACAAGGTTCTGCTGGATCAGAACTTGATTGAAGCAATGCCAACATCTCCAATCTTCGTGGGAAACAAGGCAAAGCGGCTGTTGGAGAGAGCTGTCTGGAATGATACCGCTTTCCTTGCCTCAATTGATGTGATGGATTATTCATTACTGGTCGGGGTGGATGAAGAGAAGCATGAGCTAGTACTGGGGATCATTGATTTCATGAGGCAGTATACATGGGATAAGCACCTTGAAACGTGGGTTAAAGCTTCGGGAATCCTTGGTGGGCCAAAGAACGCATCTCCGACTGTAATTTCACCAAAGCAATACAAAAAAAGGTTCAGGAAGGCCATGACAACCTATTTTCTGATGGTTCCAGATCAGTGGTCTCCTCCATCTATTGTTCCAAGTACATCCCAGTCTGATATCGGTGAAGAGAATGCACACGGTAGTGCCTCGGTTGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000285 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005768 GO:0005773 GO:0005774 GO:0005942 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006810 GO:0006996 GO:0007033 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009555 GO:0009987 GO:0010008 GO:0010118 GO:0010256 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016301 GO:0016307 GO:0016310 GO:0016482 GO:0016740 GO:0016772 GO:0016773 GO:0019637 GO:0019898 GO:0030258 GO:0031090 GO:0031410 GO:0031982 GO:0032266 GO:0032501 GO:0032502 GO:0032991 GO:0035032 GO:0035091 GO:0042147 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0048229 GO:0048856 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061695 GO:0070772 GO:0071704 GO:0071840 GO:0090332 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:1901981 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00562 ko04070 ko04145 ko04810 map00562 map04070 map04145 map04810

Pfam Domains

Protein Families

Cpn60_TCP1 FYVE PIP5K

Protein Analysis

1697

Amino Acids

187.67

Weight (kDa)

5.55

Isoelectric Point (pI)

48.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FYVE	PF01363	33 - 102	5.7e-18	FYVE zinc finger
Cpn60_TCP1	PF00118	386 - 612	2.6e-27	TCP-1/cpn60 chaperonin family
PIP5K	PF01504	1445 - 1606	1.2e-26	Phosphatidylinositol-4-phosphate 5-Kinase
PIP5K	PF01504	1605 - 1662	6.6e-08	Phosphatidylinositol-4-phosphate 5-Kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0005238)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G14270 AT3G14270
fragaria_vesca	FvH4_6g35770 FvH4_6g35770 FvH4_6g35770
malus_domestica	MD09G1173300.v1.1 MD17G1154000.v1.1
prunus_persica	Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1
pyrus_communis	pycom09g09070 pycom17g14660
rosa_chinensis	RchiOBHm_Chr2g0147811
rosa_laevigata	RLG00000020280
rosa_multiflora	Rmu_sc0000308.1_g000046
rosa_roxburghii	Rroxscaffold_2G00099330
rosa_rugosa	Rorug02G0401800 Rorug02G0401800 Rorug02G0401800 Rorug02G0401900
rosa_samantha	Rh2AG459000 Rh2BG472300 Rh2CG446400 Rh2DG481300
rosa_wichuraiana	Rw2G037540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	3 cut(s)	2440, 3913, 4429
AasI	GACNNNNNNGTC	2 cut(s)	658, 3922
AatII	GACGTC	1 cut(s)	1273
Acc36I	ACCTGC	5 cut(s)	1096, 2440, 3913, 4429, 4478
AccB1I	GGYRCC	4 cut(s)	748, 1745, 1847, 1955
AccB7I	CCANNNNNTGG	3 cut(s)	758, 3166, 3331
AccBSI	CCGCTC	1 cut(s)	3381
AccI	GTMKAC	6 cut(s)	175, 2050, 2970, 3914, 4325, 4859
AccII	CGCG	2 cut(s)	2852, 4615
AcoI	YGGCCR	4 cut(s)	1711, 2878, 3807, 4245
AcuI	CTGAAG	3 cut(s)	2265, 3090, 3203
AcyI	GRCGYC	1 cut(s)	1270
AdeI	CACNNNGTG	1 cut(s)	4034
AfeI	AGCGCT	1 cut(s)	3541
AflIII	ACRYGT	1 cut(s)	2623
AgeI	ACCGGT	2 cut(s)	2120, 3746
AhdI	GACNNNNNGTC	1 cut(s)	4297
AhlI	ACTAGT	1 cut(s)	3128
AjuI	GAANNNNNNNTTGG	6 cut(s)	2089, 2121, 2978, 3010, 3744, 3776
AleI	CACNNNNGTG	1 cut(s)	5076
AloI	GAACNNNNNNTCC	2 cut(s)	1854, 1886
Alw21I	GWGCWC	5 cut(s)	1381, 1615, 1981, 2835, 3643
Alw26I	GTCTC	7 cut(s)	1017, 1661, 1817, 1881, 1974, 2559, 5019
AlwNI	CAGNNNCTG	2 cut(s)	1940, 3800
Ama87I	CYCGRG	1 cut(s)	3048
Aor51HI	AGCGCT	1 cut(s)	3541
ArsI	GACNNNNNNTTYG	4 cut(s)	1287, 1319, 2558, 2590
AsiGI	ACCGGT	2 cut(s)	2120, 3746
Asp700I	GAANNNNTTC	3 cut(s)	270, 927, 1536
AspLEI	GCGC	2 cut(s)	1593, 3542
AvaI	CYCGRG	1 cut(s)	3048
AvaII	GGWCC	7 cut(s)	558, 1444, 1923, 3322, 3682, 3744, 3879
BaeGI	GKGCMC	1 cut(s)	1077
BaeI	ACNNNNGTAYC	2 cut(s)	4113, 4146
BalI	TGGCCA	2 cut(s)	1713, 2880
BamHI	GGATCC	3 cut(s)	54, 829, 1228
BanI	GGYRCC	4 cut(s)	748, 1745, 1847, 1955
BanII	GRGCYC	4 cut(s)	1981, 2192, 2835, 4152
BarI	GAAGNNNNNNTAC	2 cut(s)	3168, 3200
BauI	CACGAG	2 cut(s)	3787, 4359
BbsI	GAAGAC	2 cut(s)	2894, 3945
Bbv12I	GWGCWC	5 cut(s)	1381, 1615, 1981, 2835, 3643
BbvCI	CCTCAGC	1 cut(s)	3396
BceAI	ACGGC	3 cut(s)	485, 3526, 3794
BcgI	CGANNNNNNTGC	4 cut(s)	3410, 3444, 3616, 3650
BciVI	GTATCC	1 cut(s)	3012
BclI	TGATCA	1 cut(s)	2578
BcoDI	GTCTC	7 cut(s)	1017, 1661, 1817, 1881, 1974, 2559, 5019
BcuI	ACTAGT	1 cut(s)	3128
BfmI	CTRYAG	4 cut(s)	115, 960, 1122, 2391
BfoI	RGCGCY	1 cut(s)	3543
BfuAI	ACCTGC	5 cut(s)	1096, 2440, 3913, 4429, 4478
BfuI	GTATCC	1 cut(s)	3012
BglI	GCCNNNNNGGC	1 cut(s)	3836
BglII	AGATCT	4 cut(s)	355, 1696, 2715, 3107
BlpI	GCTNAGC	1 cut(s)	1095
BmcAI	AGTACT	3 cut(s)	364, 3075, 4830
Bme18I	GGWCC	7 cut(s)	558, 1444, 1923, 3322, 3682, 3744, 3879
BmeRI	GACNNNNNGTC	1 cut(s)	4297
BmeT110I	CYCGRG	1 cut(s)	3048
BmrI	ACTGGG	3 cut(s)	465, 4841, 5038
BmuI	ACTGGG	3 cut(s)	465, 4841, 5038
BpiI	GAAGAC	2 cut(s)	2894, 3945
BpmI	CTGGAG	3 cut(s)	574, 2751, 3293
Bpu10I	CCTNAGC	2 cut(s)	1527, 3396
Bpu1102I	GCTNAGC	1 cut(s)	1095
BpuEI	CTTGAG	3 cut(s)	226, 666, 1403
Bsa29I	ATCGAT	4 cut(s)	1027, 1632, 2951, 3283
BsaAI	YACGTR	1 cut(s)	1167
BsaBI	GATNNNNATC	1 cut(s)	3112
BsaHI	GRCGYC	1 cut(s)	1270
BsaI	GGTCTC	2 cut(s)	2559, 5019
BsaWI	WCCGGW	2 cut(s)	2120, 3746
BsaXI	ACNNNNNCTCC	4 cut(s)	3756, 3773, 3786, 3803
Bse118I	RCCGGY	3 cut(s)	73, 2120, 3746
Bse3DI	GCAATG	2 cut(s)	3504, 4689
Bse8I	GATNNNNATC	1 cut(s)	3112
BseCI	ATCGAT	4 cut(s)	1027, 1632, 2951, 3283
BseJI	GATNNNNATC	1 cut(s)	3112
BseMI	GCAATG	2 cut(s)	3504, 4689
BseRI	GAGGAG	4 cut(s)	1052, 3224, 4346, 5007
BseSI	GKGCMC	1 cut(s)	1077
BsgI	GTGCAG	1 cut(s)	1588
Bsh1236I	CGCG	2 cut(s)	2852, 4615
BshNI	GGYRCC	4 cut(s)	748, 1745, 1847, 1955
BshTI	ACCGGT	2 cut(s)	2120, 3746
BshVI	ATCGAT	4 cut(s)	1027, 1632, 2951, 3283
BsiHKAI	GWGCWC	5 cut(s)	1381, 1615, 1981, 2835, 3643
BsiHKCI	CYCGRG	1 cut(s)	3048
BsiSI	CCGG	4 cut(s)	74, 2121, 3747, 3836
BslFI	GGGAC	8 cut(s)	664, 1257, 2000, 2307, 2500, 3335, 3892, 4115
BsmAI	GTCTC	7 cut(s)	1017, 1661, 1817, 1881, 1974, 2559, 5019
BsmFI	GGGAC	8 cut(s)	664, 1257, 2000, 2307, 2500, 3335, 3892, 4115
BsmI	GAATGC	1 cut(s)	5071
Bso31I	GGTCTC	2 cut(s)	2559, 5019
BsoBI	CYCGRG	1 cut(s)	3048
Bsp1286I	GDGCHC	8 cut(s)	1077, 1381, 1615, 1981, 2192, 2835, 3643, 4152
Bsp1407I	TGTACA	1 cut(s)	206
Bsp1720I	GCTNAGC	1 cut(s)	1095
Bsp19I	CCATGG	1 cut(s)	39
BspDI	ATCGAT	4 cut(s)	1027, 1632, 2951, 3283
BspFNI	CGCG	2 cut(s)	2852, 4615
BspHI	TCATGA	3 cut(s)	730, 1362, 4848
BspMAI	CTGCAG	2 cut(s)	964, 2395
BspMI	ACCTGC	5 cut(s)	1096, 2440, 3913, 4429, 4478
BspQI	GCTCTTC	2 cut(s)	1996, 3678
BspT107I	GGYRCC	4 cut(s)	748, 1745, 1847, 1955
BspTNI	GGTCTC	2 cut(s)	2559, 5019
BsrBI	CCGCTC	1 cut(s)	3381
BsrDI	GCAATG	2 cut(s)	3504, 4689
BsrFI	RCCGGY	3 cut(s)	73, 2120, 3746
BsrGI	TGTACA	1 cut(s)	206
BssAI	RCCGGY	3 cut(s)	73, 2120, 3746
BssNAI	GTATAC	1 cut(s)	4860
BssNI	GRCGYC	1 cut(s)	1270
BssSI	CACGAG	2 cut(s)	3787, 4359
BssT1I	CCWWGG	6 cut(s)	39, 59, 2747, 3662, 3918, 4906
Bst1107I	GTATAC	1 cut(s)	4860
Bst2BI	CACGAG	2 cut(s)	3787, 4359
Bst6I	CTCTTC	8 cut(s)	252, 1648, 1996, 2409, 3227, 3678, 4806, 5055
BstACI	GRCGYC	1 cut(s)	1270
BstAUI	TGTACA	1 cut(s)	206
BstBAI	YACGTR	1 cut(s)	1167
BstC8I	GCNNGC	7 cut(s)	75, 1600, 1711, 2860, 2872, 2935, 3429
BstDSI	CCRYGG	2 cut(s)	39, 3408
BstFNI	CGCG	2 cut(s)	2852, 4615
BstH2I	RGCGCY	1 cut(s)	3543
BstHHI	GCGC	2 cut(s)	1593, 3542
BstMAI	GTCTC	7 cut(s)	1017, 1661, 1817, 1881, 1974, 2559, 5019
BstNSI	RCATGY	4 cut(s)	1526, 1781, 2627, 4517
BstSFI	CTRYAG	4 cut(s)	115, 960, 1122, 2391
BstSLI	GKGCMC	1 cut(s)	1077
BstSNI	TACGTA	1 cut(s)	1167
BstUI	CGCG	2 cut(s)	2852, 4615
BstV2I	GAAGAC	2 cut(s)	2894, 3945
BstXI	CCANNNNNNTGG	1 cut(s)	5010
BstZ17I	GTATAC	1 cut(s)	4860
Bsu15I	ATCGAT	4 cut(s)	1027, 1632, 2951, 3283
BsuI	GTATCC	1 cut(s)	3012
BsuTUI	ATCGAT	4 cut(s)	1027, 1632, 2951, 3283
BtgI	CCRYGG	2 cut(s)	39, 3408
BtsI	GCAGTG	1 cut(s)	3735
BtsIMutI	CAGTG	9 cut(s)	408, 1190, 2017, 2761, 3735, 4210, 4237, 4293, 5015
BveI	ACCTGC	5 cut(s)	1096, 2440, 3913, 4429, 4478
Cac8I	GCNNGC	7 cut(s)	75, 1600, 1711, 2860, 2872, 2935, 3429
CaiI	CAGNNNCTG	2 cut(s)	1940, 3800
CciI	TCATGA	3 cut(s)	730, 1362, 4848
CfoI	GCGC	2 cut(s)	1593, 3542
Cfr10I	RCCGGY	3 cut(s)	73, 2120, 3746
ClaI	ATCGAT	4 cut(s)	1027, 1632, 2951, 3283
CsiI	ACCWGGT	2 cut(s)	1939, 2695
CspAI	ACCGGT	2 cut(s)	2120, 3746
CspCI	CAANNNNNGTGG	6 cut(s)	1869, 1904, 2847, 2882, 4686, 4721
DraI	TTTAAA	1 cut(s)	1452
DraIII	CACNNNGTG	1 cut(s)	4034
DrdI	GACNNNNNNGTC	2 cut(s)	658, 3922
DriI	GACNNNNNGTC	1 cut(s)	4297
DseDI	GACNNNNNNGTC	2 cut(s)	658, 3922
EaeI	YGGCCR	4 cut(s)	1711, 2878, 3807, 4245
Eam1104I	CTCTTC	8 cut(s)	252, 1648, 1996, 2409, 3227, 3678, 4806, 5055
Eam1105I	GACNNNNNGTC	1 cut(s)	4297
EarI	CTCTTC	8 cut(s)	252, 1648, 1996, 2409, 3227, 3678, 4806, 5055
EciI	GGCGGA	2 cut(s)	1233, 4229
Ecl136II	GAGCTC	2 cut(s)	1979, 2833
Eco105I	TACGTA	1 cut(s)	1167
Eco130I	CCWWGG	6 cut(s)	39, 59, 2747, 3662, 3918, 4906
Eco24I	GRGCYC	4 cut(s)	1981, 2192, 2835, 4152
Eco31I	GGTCTC	2 cut(s)	2559, 5019
Eco32I	GATATC	1 cut(s)	5053
Eco47I	GGWCC	7 cut(s)	558, 1444, 1923, 3322, 3682, 3744, 3879
Eco47III	AGCGCT	1 cut(s)	3541
Eco53kI	GAGCTC	2 cut(s)	1979, 2833
Eco57I	CTGAAG	3 cut(s)	2265, 3090, 3203
Eco88I	CYCGRG	1 cut(s)	3048
EcoICRI	GAGCTC	2 cut(s)	1979, 2833
EcoO109I	RGGNCCY	2 cut(s)	2287, 3682
EcoRV	GATATC	1 cut(s)	5053
EcoT14I	CCWWGG	6 cut(s)	39, 59, 2747, 3662, 3918, 4906
EcoT22I	ATGCAT	3 cut(s)	1259, 2950, 4281
EcoT38I	GRGCYC	4 cut(s)	1981, 2192, 2835, 4152
ErhI	CCWWGG	6 cut(s)	39, 59, 2747, 3662, 3918, 4906
FaqI	GGGAC	8 cut(s)	664, 1257, 2000, 2307, 2500, 3335, 3892, 4115
FauI	CCCGC	1 cut(s)	998
FbaI	TGATCA	1 cut(s)	2578
FblI	GTMKAC	6 cut(s)	175, 2050, 2970, 3914, 4325, 4859
FriOI	GRGCYC	4 cut(s)	1981, 2192, 2835, 4152
GlaI	GCGC	2 cut(s)	1592, 3541
GsuI	CTGGAG	3 cut(s)	574, 2751, 3293
HaeII	RGCGCY	1 cut(s)	3543
HapII	CCGG	4 cut(s)	74, 2121, 3747, 3836
HhaI	GCGC	2 cut(s)	1593, 3542
Hin1I	GRCGYC	1 cut(s)	1270
Hin6I	GCGC	2 cut(s)	1591, 3540
HinP1I	GCGC	2 cut(s)	1591, 3540
HincII	GTYRAC	2 cut(s)	643, 3915
HindII	GTYRAC	2 cut(s)	643, 3915
HindIII	AAGCTT	2 cut(s)	3425, 4893
HpaII	CCGG	4 cut(s)	74, 2121, 3747, 3836
Hpy99I	CGWCG	2 cut(s)	638, 1332
HpyCH4IV	ACGT	3 cut(s)	1166, 1270, 4883
HpySE526I	ACGT	3 cut(s)	1166, 1270, 4883
Hsp92I	GRCGYC	1 cut(s)	1270
HspAI	GCGC	2 cut(s)	1591, 3540
KroI	GCCGGC	1 cut(s)	73
KroNI	GCCGGC	1 cut(s)	75
Ksp22I	TGATCA	1 cut(s)	2578
LguI	GCTCTTC	2 cut(s)	1996, 3678
LmnI	GCTCC	6 cut(s)	554, 797, 1039, 1976, 2830, 4370
MabI	ACCWGGT	2 cut(s)	1939, 2695
MaeII	ACGT	3 cut(s)	1166, 1270, 4883
MbiI	CCGCTC	1 cut(s)	3381
MfeI	CAATTG	1 cut(s)	4770
MhlI	GDGCHC	8 cut(s)	1077, 1381, 1615, 1981, 2192, 2835, 3643, 4152
MlsI	TGGCCA	2 cut(s)	1713, 2880
MluNI	TGGCCA	2 cut(s)	1713, 2880
MlyI	GAGTC	5 cut(s)	233, 1019, 2027, 4454, 4584
MmeI	TCCRAC	6 cut(s)	1134, 2120, 4053, 4490, 4710, 4955
Mox20I	TGGCCA	2 cut(s)	1713, 2880
Mph1103I	ATGCAT	3 cut(s)	1259, 2950, 4281
MroNI	GCCGGC	1 cut(s)	73
MroXI	GAANNNNTTC	3 cut(s)	270, 927, 1536
MscI	TGGCCA	2 cut(s)	1713, 2880
MslI	CAYNNNNRTG	5 cut(s)	1488, 2373, 2470, 4284, 5076
Msp20I	TGGCCA	2 cut(s)	1713, 2880
MspA1I	CMGCKG	2 cut(s)	1005, 3395
MspI	CCGG	4 cut(s)	74, 2121, 3747, 3836
MunI	CAATTG	1 cut(s)	4770
Mva1269I	GAATGC	1 cut(s)	5071
MvnI	CGCG	2 cut(s)	2852, 4615
NaeI	GCCGGC	1 cut(s)	75
NcoI	CCATGG	1 cut(s)	39
NgoMIV	GCCGGC	1 cut(s)	73
NmuCI	GTSAC	6 cut(s)	236, 2427, 2502, 3506, 3900, 4381
NsiI	ATGCAT	3 cut(s)	1259, 2950, 4281
NspI	RCATGY	4 cut(s)	1526, 1781, 2627, 4517
OliI	CACNNNNGTG	1 cut(s)	5076
PagI	TCATGA	3 cut(s)	730, 1362, 4848
PaqCI	CACCTGC	3 cut(s)	2440, 3913, 4429
PasI	CCCWGGG	1 cut(s)	2290
PciI	ACATGT	1 cut(s)	2623
PciSI	GCTCTTC	2 cut(s)	1996, 3678
PcsI	WCGNNNNNNNCGW	1 cut(s)	2560
PctI	GAATGC	1 cut(s)	5071
PdiI	GCCGGC	1 cut(s)	75
PdmI	GAANNNNTTC	3 cut(s)	270, 927, 1536
PflMI	CCANNNNNTGG	3 cut(s)	758, 3166, 3331
PfoI	TCCNGGA	2 cut(s)	551, 3366
PinAI	ACCGGT	2 cut(s)	2120, 3746
PleI	GAGTC	5 cut(s)	233, 1018, 2027, 4453, 4584
PpsI	GAGTC	5 cut(s)	233, 1018, 2027, 4453, 4584
Ppu21I	YACGTR	1 cut(s)	1167
PpuMI	RGGWCCY	1 cut(s)	3682
PscI	ACATGT	1 cut(s)	2623
Psp124BI	GAGCTC	2 cut(s)	1981, 2835
Psp5II	RGGWCCY	1 cut(s)	3682
PspPPI	RGGWCCY	1 cut(s)	3682
PstI	CTGCAG	2 cut(s)	964, 2395
PstNI	CAGNNNCTG	2 cut(s)	1940, 3800
PvuII	CAGCTG	1 cut(s)	3395
RseI	CAYNNNNRTG	5 cut(s)	1488, 2373, 2470, 4284, 5076
SacI	GAGCTC	2 cut(s)	1981, 2835
SalI	GTCGAC	1 cut(s)	3913
SapI	GCTCTTC	2 cut(s)	1996, 3678
ScaI	AGTACT	3 cut(s)	364, 3075, 4830
SchI	GAGTC	5 cut(s)	233, 1019, 2027, 4454, 4584
SduI	GDGCHC	8 cut(s)	1077, 1381, 1615, 1981, 2192, 2835, 3643, 4152
SexAI	ACCWGGT	2 cut(s)	1939, 2695
SfcI	CTRYAG	4 cut(s)	115, 960, 1122, 2391
SinI	GGWCC	7 cut(s)	558, 1444, 1923, 3322, 3682, 3744, 3879
SmiMI	CAYNNNNRTG	5 cut(s)	1488, 2373, 2470, 4284, 5076
SmlI	CTYRAG	3 cut(s)	241, 681, 1382
SmoI	CTYRAG	3 cut(s)	241, 681, 1382
SnaBI	TACGTA	1 cut(s)	1167
SpeI	ACTAGT	1 cut(s)	3128
SstI	GAGCTC	2 cut(s)	1981, 2835
StyI	CCWWGG	6 cut(s)	39, 59, 2747, 3662, 3918, 4906
TaiI	ACGT	3 cut(s)	1169, 1273, 4886
TatI	WGTACW	5 cut(s)	206, 362, 3073, 4828, 5036
TauI	GCSGC	5 cut(s)	408, 2855, 3384, 4250, 4726
TscAI	CASTG	9 cut(s)	415, 1197, 2017, 2761, 3742, 4210, 4237, 4300, 5015
TseFI	GTSAC	6 cut(s)	236, 2427, 2502, 3506, 3900, 4381
Tsp45I	GTSAC	6 cut(s)	236, 2427, 2502, 3506, 3900, 4381
TspGWI	ACGGA	2 cut(s)	2415, 2544
TspRI	CASTG	9 cut(s)	415, 1197, 2017, 2761, 3742, 4210, 4237, 4300, 5015
Van91I	CCANNNNNTGG	3 cut(s)	758, 3166, 3331
VpaK11BI	GGWCC	7 cut(s)	558, 1444, 1923, 3322, 3682, 3744, 3879
XbaI	TCTAGA	1 cut(s)	352
XceI	RCATGY	4 cut(s)	1526, 1781, 2627, 4517
XcmI	CCANNNNNNNNNTGG	1 cut(s)	4393
XmiI	GTMKAC	6 cut(s)	175, 2050, 2970, 3914, 4325, 4859
XmnI	GAANNNNTTC	3 cut(s)	270, 927, 1536
ZraI	GACGTC	1 cut(s)	1271
ZrmI	AGTACT	3 cut(s)	364, 3075, 4830
Zsp2I	ATGCAT	3 cut(s)	1259, 2950, 4281

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.