Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2C

Orthogroup ID

OG0005238

Physical Location & Seq

Genomic Coordinates Forward (+)

60470708 .. 60478425

Gene Structure 7718 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2CG446400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5463 bp

ATGGCTGCCCCCAATAAGTTTTTTTCTCAGTTTATTTCCATGGTGAAATCATGGATCCCTTGGCGATCTGAGCCGGCTAATGTTTCGAGGGATTTTTGGATGCCTGATGATAGCTGTAGGGTATGTTATGAGTGTGATGCACAGTTCACTGTATTTAACCGTAAACATCATTGTAGACTTTGTGGACGAGTTTTCTGTGCCAAGTGTACAGAAAACTCAATTCCTGCCCCCTCTGATGACTCAAGGATAGATCGGGAAGAGAGGGAAAGGATTCGTGTCTGCAATTTTTGTTACAAGCAACATCAGCAAGGCATAGCTGCTACTCCTGATAATGGAACACAGATTGCTAATCTAGATCTTAGTACCTCACCATCAGAAACAAGTTTTACTAGCTTTAAGTCATGCGGCACTGGTAACAGCAGTAGCTTTACTCTTAACTCAATCCCGTACTCAACTGGGCCATACCAACGACTTCAAAACAGTTCTGGTCTTAGCCCCTGTCAATCATCTCCCATGAAAACAAGCTCAGAAAAGCACAGCAAATATGCCTCCTGGAGGACCAATGATTTTGTTGCAGATATAGAGGATTCATCTCCAAATCATTATGAGATTTCAACAACCAGCATCAGGAGTGATGACGACGATGTTGACTATGGGACATATCAGTCAGATTCTAAGAATTATTCTCAAGTCAATGATTACTATGGTCATGTTGAGTTTTATGAGATGAGCAATCATGATGAATCACATAAGGTGCAACTTGATGGTGAAAACATTGATGCAAAGCATTTAAGCAGCTCCCCGTTACTTCACAGTTTTAACTCGCAGGGTTCGGATCCACCACTTGAAAAGAAAGAAGATGAACATGATATGGGTGATGAATGTGCTTCCTCTTTATGTTCTGCAGGGGATGTTGACGTAGAACCTGTGGATTTTGAGAACAATGCCCTTCTTTGGCTTCCCCCTGAACCAGAAGATGAAGAAGATGAAAGGGAAACTGTTCTTCTTGATGATGACGATGATGGTGATGCTGCAGGAGAGTGGGGAACCTTACGCACATCAAGCAGTTTTGGCAGCGGGGAGTCTCGCAATAGGGATCGATCAGGTGAGGAGCATAAGAAGGTGATGAAGAATGTGGTTGATGGGCACTTTAGGGCTTTGGTAGCTCAGCTATTGCAGGTTGAGAACCTCCCTGTAGGCCAGGAAGGTGAAAATGAAAGTTGGTTGGAGATCATTACGTATCTGTCTTGGGAGGCTGCTACACTGTTAAAGCCAGATATGAGCAAAGGTGGAGGGATGGATCCTGGTGGTTATGTAAAAGTAAAATGCATAGCTTCTGGACGTCCCTCTGACAGTATGGTGGTCAAAGGAGTTGTCTGTAAGAAAAATGTGGCTCATCGACGAATGGCATCTAAAATGGAGAAACCTCGGTTCATGATTCTTGGTGGTGCGCTTGAGTACCAGCGAGTTTCTAACCTATTGTCAAGTTTTGATACTCTTTTACAGCAGGAGATGGACCATTTAAAGATGGCAGTAGCAAAGATTGAAGCGCACCACCCTGATGTCCTTCTGGTGGAGAAATCAGTTTCTCGATATGCTCAGGAATATCTTCTTGCAAAAGACATATCACTTGTTCTTAATATCAAGAGGCCACTTTTAGAGCGCATAGCTCGCTGCACAGGTGCTCAAATAGTTCCATCTATCGATCATCTCTCTTCACAAAAGTTGGGCTACTGTGAGACATTTCATGTGGGGAGGTTTCTGGAAGATCTTGGTTCTGCTGGCCAGGGTGGGAAGAAACTGGTGAAGACATTGATGTATTTTGAAGGCTGCCCAAAACCATTGGGTTGTACTATTTTACTCAGAGGTGCCAATGGGGATGAGTTGAAGAAAGTGAAGCATGTGGTTCAGTATGGGGTTTTTGCAGCATATCACTTGGCTTTGGAGACATCCTTCCTTGCTGATGAAGGTGCCTCTCTGCCTGAACTCCCATTCCAGTCTCCAATTACTGTGGCACTTCCAGATAAACCATCGAGTATTGAGAGGTCCATCTCAACAGTACCTGGTTTTAGAATTGGTGCCAACGAAAAGTCTCTGGGAGCTCAACCTCAGAGTGAACCACGAAGAGCCAACAGTGTCCCGATTACAGATTTTGACTCAGCAATTAAGAGTAGACCACCATGTTTACTAACTGGTCGTAGCTCTCTACCTGTTCGTCCGACTTCAAGTTCCACAAATTCTACCGGTTTACACTCTGCCCCACCTGGGAACGGTGTTACTTTTCATATCGCTGATAATCAAAATGAAATGGGCCCCAAAGATTCTTGGGTGGTGGAAACTTCTGCAACCAAACCTGGCTCTGATAATATGTCTAATCATCTTACTGCAAATAGCATGGGATCTTCAGAGGCCCTGGGACAAGGTATCTTCTCCAATACCCAAAATGATCCAAGTGTAAATCAGCTAGGTAGTTCAGGCAATCCAATGCTGCATCAAGATGGTCAAACCCATGCTGCAGATGTAGGAACCATGAATGAAGAGTTCCCTCCGTCACCTGCTGACCATCAGAGCATTTTAGTTTCCTTGTCATCTCGATGCGTGTGGAAGGGGACTGTCTGTGAGAGGTCACATCTGTTCCGAATTAAATACTACGGAAGTTTTGATAAACCTTTAGGACGGTTTTTGCGAGACCATTTATTTGATCAGACCTATCAGTGCCATTCTTGCGAGATGCCATCAGAGGCACATGTTCATTGTTATACTCACCGACAGGGTACACTCACTATATCTGTTAAGAGGCTGCCAGAAAATTTCTTACCAGGTGAAAGGGAAGGGAAGATCTGGATGTGGCACAGATGTTTGAGGTGTCCAAGGATCAGTGGATTTCCTCCAGCTACTCGGAGAATAGTGATGTCTGATGCTGCATGGGGTTTGTCATTTGGGAAGTTTTTGGAGCTCAGTTTTTCAAACCACGCGGCTGCAAGCAGGGTGGCGAGCTGTGGCCATTCCTTACATAGAGATTGTCTTCGTTTCTATGGATTTGGGAAAATGGTTGCTTGCTTTCGGTATGCATCGATTGATGTTCATTCTGTCTACCTTCCACCCTCCAAACTGGATTTCATTTCCGAGAAGCAGGAGTGGATACAAAAAGAAACTAATGAGGTTGTTGCCCGAGCAGAGCTTCTTTTCTCTGAAGTACTTAATGCTCTTAGTCAAATTTCAGAGAAAAGATCTGGTTCAGGGTCAATTACTAGTGGCATTGTAACAGCTGAATCCAGACACCAAATAGTGGAACTGGAAGGAATGTTACAGAAGGAGAAGGTGGAATTTGAGGAGTTACTTCAGAAAACTTTGAAGAGAGAACCTAAGAAGGGCCAACCTGTTATCGACATTCTGGAGATCAATCGATTGCGGAGGCAGTTGTTTTTTCAATCTTATATGTGGGACCACCGCTTGGTTTATGCAGCCAGTTTAGATAACAACAGCTTCCAGGATAGTTTGAGCAGCTCAATTCCAGCTGAGGATAAACCCATGGCTACTAATGAAAAGCTTGCTGAGATGGATGTAGAAATAAAGCCAGGAAAAGGCTATAATAGTTGTGATTCCTTTCTGGTGGATGCAATGCTTAGTGATGGCTTTGACCACAAGGGAGGCTTTACTAGCGCTGCTATCAATGCAGACATGGTTCATGGAGAGGACATGAGTGATGATTTGAATAAGGAAAAAGGTCAAGCTATTCTCCCTACTAGCACAAGTGTCGGCGCTCAATCTGATCCTTTGACACCAAGGACAGCTCTTCGTAGGGTCCTATCTGATGGTCAGCATCCTCTCATGCTGAATTTGTCAGATACCCTTGACACTGCATGGACCGGCGAAAATCTCATTGGAGTTGCGATAGCAAAGGAGAACTCATGTCCAGTTCCTGACGTGGCCATAGAAAATTCTTCAAATGCCTCTCCGGCAGAGGGATTAAATTTAGACCATGCAGAAGACCAAAATGGGACCAAAGTTGCCCATTATGTGTCACCTGCATTGTCGACCAAGGGGTCTGAGAATATGGAAGACACAGTTAGCTGGTTAAAAATGCCCTTTTTGAACTTCTACCGTTCACTAAATAAGAATTTTTTATCTGCTGCTCAGAAGTTCGATACACTTGGTGGGTACAATCCAGTCTATGTGTCATCCTTTCGGGAGTTGGAACTGGAAGGTGGTGCTAGGCTGCTCTTGCCTGTGGGTGAAAACGATACTGTTGTCCCTGTATATGATGATGAGCCCACAAGTCTTATAGCCTATGCCCTTGTTTCATCAGATTATCAGTTACAAACCAGTGATGAGGGGGAAAGAGCAAAAGACAGTGGGGATATTATGGCCACAGTATCTTTTACTGATTCAGTCATTATGCATCCAGATGATGACACTGTGTCTGAAACTCATAGAAGTCTTGGGTCTACTGAAGAGAGCATACTATCAATGTCTGGATCTCGTGGCTCCCTGGGTTTGGATCCACTTTTATATACAAAGGCTTTGCATGCAAGAGTGTCTTTTGGAGATGATGGTCCTCTTGGTAAGGTTAAGTACTCTGTCACATGTTATTATGCAAAGCGTTTTGAAGCCTTAAGGAAGATGTGTTGTCCCTCTGAGCTTGATTTTGTAAGATCCCTAAGTCGTTGTAAAAAGTGGGGAGCCCAAGGAGGTAAGAGCAATGTCTTTTTTGCAAAAACCTTGGATGACCGGTTTATCATCAAACAAGTCACCAAGACCGAGCTGGAATCTTTCATTAAATTTGCACCTGCATATTTCAAGTACCTCTCTGAGTCAATTAGCACAGGAAGTCCAACCTGCCTGGCAAAAATTCTGGGGATATATCAGGTTATGTCGAAGCATGTTAAGGGAGGCAAAGAAACAAAGATGGATGTTTTGATTATGGAGAATCTTCTGTTTGGAAGGACTGTAACACGACTCTATGATCTCAAAGGATCTTCGCGGTCAAGGTATAATCCTGATTCTAGTGGGAGTAACAAGGTTCTGCTGGATCAGAACTTGATTGAAGCAATGCCAACATCTCCAATCTTCGTGGGAAACAAGGCAAAGCGGCTGTTGGAGAGAGCTGTCTGGAATGATACCGCTTTCCTTGCCTCAATTGATGTGATGGATTATTCATTACTGGTCGGGGTGGATGAAGAGAAGCATGAGCTAGTACTTGGGATCATTGATTTCATGAGGCAGTATACATGGGATAAGCACCTTGAAACGTGGGTTAAAGCTTCAGGAATCCTTGGTGGGCCAAAGAACGCATCTCCGACTGTAATTTCACCAAAGCAATACAAAAAAAGGTTCAGGAAGGCCATGACAACCTATTTTCTGATGGTTCCAGATCAGTGGTCTCCTCCATCTATTGTTCCAAGTACATCCCAGTCTGATATCGGTGAAGAGAATGCACACGGTGGTGCCTCGGTTGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000285 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005768 GO:0005773 GO:0005774 GO:0005942 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006810 GO:0006996 GO:0007033 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009555 GO:0009987 GO:0010008 GO:0010118 GO:0010256 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016301 GO:0016307 GO:0016310 GO:0016482 GO:0016740 GO:0016772 GO:0016773 GO:0019637 GO:0019898 GO:0030258 GO:0031090 GO:0031410 GO:0031982 GO:0032266 GO:0032501 GO:0032502 GO:0032991 GO:0035032 GO:0035091 GO:0042147 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0048229 GO:0048856 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061695 GO:0070772 GO:0071704 GO:0071840 GO:0090332 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:1901981 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00562 ko04070 ko04145 ko04810 map00562 map04070 map04145 map04810

Pfam Domains

Protein Families

Cpn60_TCP1 FYVE PIP5K

Protein Analysis

1820

Amino Acids

201.55

Weight (kDa)

5.62

Isoelectric Point (pI)

48.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FYVE	PF01363	33 - 102	8.2e-18	FYVE zinc finger
Cpn60_TCP1	PF00118	410 - 653	1.7e-30	TCP-1/cpn60 chaperonin family
PIP5K	PF01504	1563 - 1729	1.4e-33	Phosphatidylinositol-4-phosphate 5-Kinase
PIP5K	PF01504	1728 - 1785	7.1e-08	Phosphatidylinositol-4-phosphate 5-Kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005238)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G14270 AT3G14270
fragaria_vesca	FvH4_6g35770 FvH4_6g35770 FvH4_6g35770
malus_domestica	MD09G1173300.v1.1 MD17G1154000.v1.1
prunus_persica	Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1
pyrus_communis	pycom09g09070 pycom17g14660
rosa_chinensis	RchiOBHm_Chr2g0147811
rosa_laevigata	RLG00000020280
rosa_multiflora	Rmu_sc0000308.1_g000046
rosa_roxburghii	Rroxscaffold_2G00099330
rosa_rugosa	Rorug02G0401800 Rorug02G0401800 Rorug02G0401800 Rorug02G0401900
rosa_samantha	Rh2AG459000 Rh2BG472300 Rh2CG446400 Rh2DG481300
rosa_wichuraiana	Rw2G037540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	3 cut(s)	2563, 4036, 4798
AasI	GACNNNNNNGTC	2 cut(s)	664, 4045
AatII	GACGTC	1 cut(s)	1345
Acc36I	ACCTGC	5 cut(s)	1168, 2563, 4036, 4798, 4847
AccB1I	GGYRCC	4 cut(s)	1868, 1970, 2078, 5447
AccB7I	CCANNNNNTGG	2 cut(s)	3289, 3454
AccI	GTMKAC	6 cut(s)	175, 2173, 3093, 4037, 4448, 5228
AccII	CGCG	2 cut(s)	2975, 4984
AciI	CCGC	8 cut(s)	405, 1077, 2975, 3412, 3451, 4984, 5092, 5124
AcoI	YGGCCR	4 cut(s)	1783, 3001, 3930, 4368
AcuI	CTGAAG	5 cut(s)	2388, 3213, 3326, 4473, 5250
AcyI	GRCGYC	1 cut(s)	1342
AdeI	CACNNNGTG	2 cut(s)	4157, 5444
AfeI	AGCGCT	1 cut(s)	3664
AflII	CTTAAG	1 cut(s)	4615
AflIII	ACRYGT	2 cut(s)	2746, 4586
AgeI	ACCGGT	2 cut(s)	2243, 4731
AhdI	GACNNNNNGTC	1 cut(s)	4420
AhlI	ACTAGT	1 cut(s)	3251
AjiI	CACGTC	1 cut(s)	3928
AjuI	GAANNNNNNNTTGG	4 cut(s)	3101, 3133, 3867, 3899
AleI	CACNNNNGTG	1 cut(s)	5445
AloI	GAACNNNNNNTCC	2 cut(s)	1977, 2009
Alw21I	GWGCWC	3 cut(s)	1687, 2104, 2958
Alw26I	GTCTC	7 cut(s)	1089, 1733, 1940, 2004, 2097, 2682, 5388
AlwNI	CAGNNNCTG	2 cut(s)	2063, 3923
Ama87I	CYCGRG	1 cut(s)	3171
Aor51HI	AGCGCT	1 cut(s)	3664
ApaI	GGGCCC	1 cut(s)	2315
ArsI	GACNNNNNNTTYG	4 cut(s)	1359, 1391, 2681, 2713
AsiGI	ACCGGT	2 cut(s)	2243, 4731
Asp700I	GAANNNNTTC	3 cut(s)	270, 999, 1608
AspLEI	GCGC	5 cut(s)	1453, 1552, 1665, 3665, 3764
AvaI	CYCGRG	1 cut(s)	3171
AvaII	GGWCC	8 cut(s)	558, 1516, 2046, 3445, 3805, 3867, 4002, 4556
BaeGI	GKGCMC	2 cut(s)	1149, 2315
BaeI	ACNNNNGTAYC	2 cut(s)	4236, 4269
BalI	TGGCCA	4 cut(s)	1785, 3003, 3932, 4370
BamHI	GGATCC	4 cut(s)	54, 835, 1300, 4501
BanI	GGYRCC	4 cut(s)	1868, 1970, 2078, 5447
BanII	GRGCYC	5 cut(s)	2104, 2315, 2958, 4275, 4687
BarI	GAAGNNNNNNTAC	4 cut(s)	3291, 3323, 4446, 4478
BauI	CACGAG	1 cut(s)	4482
BbsI	GAAGAC	4 cut(s)	1814, 3017, 3996, 4068
Bbv12I	GWGCWC	3 cut(s)	1687, 2104, 2958
BbvCI	CCTCAGC	1 cut(s)	3519
BcgI	CGANNNNNNTGC	2 cut(s)	3739, 3773
BciVI	GTATCC	1 cut(s)	3135
BclI	TGATCA	1 cut(s)	2701
BcoDI	GTCTC	7 cut(s)	1089, 1733, 1940, 2004, 2097, 2682, 5388
BcuI	ACTAGT	1 cut(s)	3251
BfaI	CTAG	9 cut(s)	353, 390, 2465, 3252, 3660, 3747, 4215, 5007, 5195
BfmI	CTRYAG	5 cut(s)	115, 903, 1032, 1194, 2514
BfoI	RGCGCY	2 cut(s)	3666, 3765
BfrI	CTTAAG	1 cut(s)	4615
BfuAI	ACCTGC	5 cut(s)	1168, 2563, 4036, 4798, 4847
BfuI	GTATCC	1 cut(s)	3135
BglI	GCCNNNNNGGC	1 cut(s)	3959
BglII	AGATCT	4 cut(s)	355, 1768, 2838, 3230
BlpI	GCTNAGC	1 cut(s)	1167
BmcAI	AGTACT	3 cut(s)	3198, 4577, 5199
Bme18I	GGWCC	8 cut(s)	558, 1516, 2046, 3445, 3805, 3867, 4002, 4556
BmeRI	GACNNNNNGTC	1 cut(s)	4420
BmeT110I	CYCGRG	1 cut(s)	3171
BmgBI	CACGTC	1 cut(s)	3928
BmrI	ACTGGG	2 cut(s)	465, 5407
BmuI	ACTGGG	2 cut(s)	465, 5407
BpiI	GAAGAC	4 cut(s)	1814, 3017, 3996, 4068
BpmI	CTGGAG	3 cut(s)	574, 2874, 3416
Bpu10I	CCTNAGC	2 cut(s)	1599, 3519
Bpu1102I	GCTNAGC	1 cut(s)	1167
BpuEI	CTTGAG	3 cut(s)	226, 672, 1475
Bsa29I	ATCGAT	4 cut(s)	1099, 1704, 3074, 3406
BsaAI	YACGTR	1 cut(s)	1239
BsaHI	GRCGYC	1 cut(s)	1342
BsaI	GGTCTC	2 cut(s)	2682, 5388
BsaWI	WCCGGW	2 cut(s)	2243, 4731
Bse118I	RCCGGY	4 cut(s)	73, 2243, 3869, 4731
Bse3DI	GCAATG	3 cut(s)	3627, 4708, 5058
BseCI	ATCGAT	4 cut(s)	1099, 1704, 3074, 3406
BseMI	GCAATG	3 cut(s)	3627, 4708, 5058
BseRI	GAGGAG	3 cut(s)	1124, 3347, 5376
BseSI	GKGCMC	2 cut(s)	1149, 2315
BsgI	GTGCAG	1 cut(s)	1660
Bsh1236I	CGCG	2 cut(s)	2975, 4984
BshNI	GGYRCC	4 cut(s)	1868, 1970, 2078, 5447
BshTI	ACCGGT	2 cut(s)	2243, 4731
BshVI	ATCGAT	4 cut(s)	1099, 1704, 3074, 3406
BsiHKAI	GWGCWC	3 cut(s)	1687, 2104, 2958
BsiHKCI	CYCGRG	1 cut(s)	3171
BsiSI	CCGG	5 cut(s)	74, 2244, 3870, 3959, 4732
BslFI	GGGAC	9 cut(s)	670, 1329, 2123, 2430, 2623, 3458, 4015, 4238, 4617
BsmAI	GTCTC	7 cut(s)	1089, 1733, 1940, 2004, 2097, 2682, 5388
BsmFI	GGGAC	9 cut(s)	670, 1329, 2123, 2430, 2623, 3458, 4015, 4238, 4617
BsmI	GAATGC	1 cut(s)	5440
Bso31I	GGTCTC	2 cut(s)	2682, 5388
BsoBI	CYCGRG	1 cut(s)	3171
Bsp120I	GGGCCC	1 cut(s)	2311
Bsp1286I	GDGCHC	7 cut(s)	1149, 1687, 2104, 2315, 2958, 4275, 4687
Bsp1407I	TGTACA	1 cut(s)	206
Bsp1720I	GCTNAGC	1 cut(s)	1167
Bsp19I	CCATGG	2 cut(s)	39, 3531
BspACI	CCGC	8 cut(s)	405, 1077, 2975, 3412, 3451, 4984, 5092, 5124
BspDI	ATCGAT	4 cut(s)	1099, 1704, 3074, 3406
BspFNI	CGCG	2 cut(s)	2975, 4984
BspHI	TCATGA	3 cut(s)	736, 1434, 5217
BspMAI	CTGCAG	3 cut(s)	907, 1036, 2518
BspMI	ACCTGC	5 cut(s)	1168, 2563, 4036, 4798, 4847
BspQI	GCTCTTC	2 cut(s)	2119, 3801
BspT107I	GGYRCC	4 cut(s)	1868, 1970, 2078, 5447
BspTI	CTTAAG	1 cut(s)	4615
BspTNI	GGTCTC	2 cut(s)	2682, 5388
BsrDI	GCAATG	3 cut(s)	3627, 4708, 5058
BsrFI	RCCGGY	4 cut(s)	73, 2243, 3869, 4731
BsrGI	TGTACA	1 cut(s)	206
BssAI	RCCGGY	4 cut(s)	73, 2243, 3869, 4731
BssNAI	GTATAC	1 cut(s)	5229
BssNI	GRCGYC	1 cut(s)	1342
BssSI	CACGAG	1 cut(s)	4482
BssT1I	CCWWGG	9 cut(s)	39, 59, 2870, 3531, 3785, 4041, 4687, 4722, 5275
Bst1107I	GTATAC	1 cut(s)	5229
Bst2BI	CACGAG	1 cut(s)	4482
Bst6I	CTCTTC	9 cut(s)	252, 1720, 2119, 2532, 3350, 3801, 4449, 5175, 5424
BstACI	GRCGYC	1 cut(s)	1342
BstAFI	CTTAAG	1 cut(s)	4615
BstAUI	TGTACA	1 cut(s)	206
BstBAI	YACGTR	1 cut(s)	1239
BstC8I	GCNNGC	8 cut(s)	75, 1672, 1783, 2983, 2995, 3058, 3552, 4530
BstDSI	CCRYGG	2 cut(s)	39, 3531
BstENI	CCTNNNNNAGG	1 cut(s)	553
BstFNI	CGCG	2 cut(s)	2975, 4984
BstH2I	RGCGCY	2 cut(s)	3666, 3765
BstHHI	GCGC	5 cut(s)	1453, 1552, 1665, 3665, 3764
BstMAI	GTCTC	7 cut(s)	1089, 1733, 1940, 2004, 2097, 2682, 5388
BstNSI	RCATGY	5 cut(s)	1904, 2750, 4532, 4590, 4886
BstSFI	CTRYAG	5 cut(s)	115, 903, 1032, 1194, 2514
BstSLI	GKGCMC	2 cut(s)	1149, 2315
BstSNI	TACGTA	1 cut(s)	1239
BstUI	CGCG	2 cut(s)	2975, 4984
BstV2I	GAAGAC	4 cut(s)	1814, 3017, 3996, 4068
BstXI	CCANNNNNNTGG	1 cut(s)	5379
BstZ17I	GTATAC	1 cut(s)	5229
Bsu15I	ATCGAT	4 cut(s)	1099, 1704, 3074, 3406
BsuI	GTATCC	1 cut(s)	3135
BsuTUI	ATCGAT	4 cut(s)	1099, 1704, 3074, 3406
BtgI	CCRYGG	2 cut(s)	39, 3531
BtrI	CACGTC	1 cut(s)	3928
BtsI	GCAGTG	1 cut(s)	3858
BveI	ACCTGC	5 cut(s)	1168, 2563, 4036, 4798, 4847
Cac8I	GCNNGC	8 cut(s)	75, 1672, 1783, 2983, 2995, 3058, 3552, 4530
CaiI	CAGNNNCTG	2 cut(s)	2063, 3923
CciI	TCATGA	3 cut(s)	736, 1434, 5217
CfoI	GCGC	5 cut(s)	1453, 1552, 1665, 3665, 3764
Cfr10I	RCCGGY	4 cut(s)	73, 2243, 3869, 4731
ClaI	ATCGAT	4 cut(s)	1099, 1704, 3074, 3406
CsiI	ACCWGGT	2 cut(s)	2062, 2818
CspAI	ACCGGT	2 cut(s)	2243, 4731
CspCI	CAANNNNNGTGG	6 cut(s)	1992, 2027, 2970, 3005, 5055, 5090
DraI	TTTAAA	1 cut(s)	1524
DraIII	CACNNNGTG	2 cut(s)	4157, 5444
DrdI	GACNNNNNNGTC	2 cut(s)	664, 4045
DriI	GACNNNNNGTC	1 cut(s)	4420
DseDI	GACNNNNNNGTC	2 cut(s)	664, 4045
EaeI	YGGCCR	4 cut(s)	1783, 3001, 3930, 4368
Eam1104I	CTCTTC	9 cut(s)	252, 1720, 2119, 2532, 3350, 3801, 4449, 5175, 5424
Eam1105I	GACNNNNNGTC	1 cut(s)	4420
EarI	CTCTTC	9 cut(s)	252, 1720, 2119, 2532, 3350, 3801, 4449, 5175, 5424
Ecl136II	GAGCTC	2 cut(s)	2102, 2956
Eco105I	TACGTA	1 cut(s)	1239
Eco130I	CCWWGG	9 cut(s)	39, 59, 2870, 3531, 3785, 4041, 4687, 4722, 5275
Eco24I	GRGCYC	5 cut(s)	2104, 2315, 2958, 4275, 4687
Eco31I	GGTCTC	2 cut(s)	2682, 5388
Eco32I	GATATC	1 cut(s)	5422
Eco47I	GGWCC	8 cut(s)	558, 1516, 2046, 3445, 3805, 3867, 4002, 4556
Eco47III	AGCGCT	1 cut(s)	3664
Eco53kI	GAGCTC	2 cut(s)	2102, 2956
Eco57I	CTGAAG	5 cut(s)	2388, 3213, 3326, 4473, 5250
Eco88I	CYCGRG	1 cut(s)	3171
EcoICRI	GAGCTC	2 cut(s)	2102, 2956
EcoNI	CCTNNNNNAGG	1 cut(s)	553
EcoO109I	RGGNCCY	3 cut(s)	2312, 2410, 3805
EcoRV	GATATC	1 cut(s)	5422
EcoT14I	CCWWGG	9 cut(s)	39, 59, 2870, 3531, 3785, 4041, 4687, 4722, 5275
EcoT22I	ATGCAT	3 cut(s)	1331, 3073, 4404
EcoT38I	GRGCYC	5 cut(s)	2104, 2315, 2958, 4275, 4687
ErhI	CCWWGG	9 cut(s)	39, 59, 2870, 3531, 3785, 4041, 4687, 4722, 5275
FalI	AAGNNNNNCTT	4 cut(s)	3638, 3670, 4525, 4557
FaqI	GGGAC	9 cut(s)	670, 1329, 2123, 2430, 2623, 3458, 4015, 4238, 4617
FauI	CCCGC	1 cut(s)	1070
FbaI	TGATCA	1 cut(s)	2701
FblI	GTMKAC	6 cut(s)	175, 2173, 3093, 4037, 4448, 5228
FriOI	GRGCYC	5 cut(s)	2104, 2315, 2958, 4275, 4687
FspBI	CTAG	9 cut(s)	353, 390, 2465, 3252, 3660, 3747, 4215, 5007, 5195
GlaI	GCGC	5 cut(s)	1452, 1551, 1664, 3664, 3763
GsuI	CTGGAG	3 cut(s)	574, 2874, 3416
HaeII	RGCGCY	2 cut(s)	3666, 3765
HapII	CCGG	5 cut(s)	74, 2244, 3870, 3959, 4732
HhaI	GCGC	5 cut(s)	1453, 1552, 1665, 3665, 3764
Hin1I	GRCGYC	1 cut(s)	1342
Hin6I	GCGC	5 cut(s)	1451, 1550, 1663, 3663, 3762
HinP1I	GCGC	5 cut(s)	1451, 1550, 1663, 3663, 3762
HincII	GTYRAC	3 cut(s)	649, 916, 4038
HindII	GTYRAC	3 cut(s)	649, 916, 4038
HindIII	AAGCTT	2 cut(s)	3548, 5262
HpaII	CCGG	5 cut(s)	74, 2244, 3870, 3959, 4732
Hpy99I	CGWCG	2 cut(s)	644, 1404
HpyCH4IV	ACGT	5 cut(s)	918, 1238, 1342, 3927, 5252
HpySE526I	ACGT	5 cut(s)	918, 1238, 1342, 3927, 5252
Hsp92I	GRCGYC	1 cut(s)	1342
HspAI	GCGC	5 cut(s)	1451, 1550, 1663, 3663, 3762
KroI	GCCGGC	1 cut(s)	73
KroNI	GCCGGC	1 cut(s)	75
Ksp22I	TGATCA	1 cut(s)	2701
LguI	GCTCTTC	2 cut(s)	2119, 3801
LmnI	GCTCC	6 cut(s)	803, 1111, 2099, 2953, 4493, 4682
MabI	ACCWGGT	2 cut(s)	2062, 2818
MaeI	CTAG	9 cut(s)	353, 390, 2465, 3252, 3660, 3747, 4215, 5007, 5195
MaeII	ACGT	5 cut(s)	918, 1238, 1342, 3927, 5252
MfeI	CAATTG	1 cut(s)	5139
MhlI	GDGCHC	7 cut(s)	1149, 1687, 2104, 2315, 2958, 4275, 4687
MlsI	TGGCCA	4 cut(s)	1785, 3003, 3932, 4370
MluNI	TGGCCA	4 cut(s)	1785, 3003, 3932, 4370
MlyI	GAGTC	5 cut(s)	233, 1091, 2150, 4823, 4953
MmeI	TCCRAC	6 cut(s)	1206, 2243, 4176, 4859, 5079, 5324
Mox20I	TGGCCA	4 cut(s)	1785, 3003, 3932, 4370
Mph1103I	ATGCAT	3 cut(s)	1331, 3073, 4404
MroNI	GCCGGC	1 cut(s)	73
MroXI	GAANNNNTTC	3 cut(s)	270, 999, 1608
MscI	TGGCCA	4 cut(s)	1785, 3003, 3932, 4370
MslI	CAYNNNNRTG	5 cut(s)	1560, 2496, 2593, 4407, 5445
Msp20I	TGGCCA	4 cut(s)	1785, 3003, 3932, 4370
MspA1I	CMGCKG	3 cut(s)	1077, 3269, 3518
MspCI	CTTAAG	1 cut(s)	4615
MspI	CCGG	5 cut(s)	74, 2244, 3870, 3959, 4732
MunI	CAATTG	1 cut(s)	5139
Mva1269I	GAATGC	1 cut(s)	5440
MvnI	CGCG	2 cut(s)	2975, 4984
NaeI	GCCGGC	1 cut(s)	75
NcoI	CCATGG	2 cut(s)	39, 3531
NgoMIV	GCCGGC	1 cut(s)	73
NmuCI	GTSAC	5 cut(s)	2550, 2625, 4023, 4582, 4750
NsiI	ATGCAT	3 cut(s)	1331, 3073, 4404
NspI	RCATGY	5 cut(s)	1904, 2750, 4532, 4590, 4886
OliI	CACNNNNGTG	1 cut(s)	5445
PaeI	GCATGC	1 cut(s)	4532
PagI	TCATGA	3 cut(s)	736, 1434, 5217
PaqCI	CACCTGC	3 cut(s)	2563, 4036, 4798
PasI	CCCWGGG	2 cut(s)	2413, 4492
PciI	ACATGT	2 cut(s)	2746, 4586
PciSI	GCTCTTC	2 cut(s)	2119, 3801
PcsI	WCGNNNNNNNCGW	1 cut(s)	2683
PctI	GAATGC	1 cut(s)	5440
PdiI	GCCGGC	1 cut(s)	75
PdmI	GAANNNNTTC	3 cut(s)	270, 999, 1608
PflMI	CCANNNNNTGG	2 cut(s)	3289, 3454
PfoI	TCCNGGA	2 cut(s)	551, 3489
PinAI	ACCGGT	2 cut(s)	2243, 4731
PleI	GAGTC	5 cut(s)	233, 1090, 2150, 4822, 4953
PpsI	GAGTC	5 cut(s)	233, 1090, 2150, 4822, 4953
Ppu21I	YACGTR	1 cut(s)	1239
PpuMI	RGGWCCY	1 cut(s)	3805
PscI	ACATGT	2 cut(s)	2746, 4586
Psp124BI	GAGCTC	2 cut(s)	2104, 2958
Psp5II	RGGWCCY	1 cut(s)	3805
PspOMI	GGGCCC	1 cut(s)	2311
PspPPI	RGGWCCY	1 cut(s)	3805
PstI	CTGCAG	3 cut(s)	907, 1036, 2518
PstNI	CAGNNNCTG	2 cut(s)	2063, 3923
PvuII	CAGCTG	2 cut(s)	3269, 3518
RseI	CAYNNNNRTG	5 cut(s)	1560, 2496, 2593, 4407, 5445
SacI	GAGCTC	2 cut(s)	2104, 2958
SalI	GTCGAC	1 cut(s)	4036
SapI	GCTCTTC	2 cut(s)	2119, 3801
ScaI	AGTACT	3 cut(s)	3198, 4577, 5199
SchI	GAGTC	5 cut(s)	233, 1091, 2150, 4823, 4953
SduI	GDGCHC	7 cut(s)	1149, 1687, 2104, 2315, 2958, 4275, 4687
SexAI	ACCWGGT	2 cut(s)	2062, 2818
SfcI	CTRYAG	5 cut(s)	115, 903, 1032, 1194, 2514
SinI	GGWCC	8 cut(s)	558, 1516, 2046, 3445, 3805, 3867, 4002, 4556
SmiMI	CAYNNNNRTG	5 cut(s)	1560, 2496, 2593, 4407, 5445
SmlI	CTYRAG	4 cut(s)	241, 687, 1454, 4615
SmoI	CTYRAG	4 cut(s)	241, 687, 1454, 4615
SnaBI	TACGTA	1 cut(s)	1239
SpeI	ACTAGT	1 cut(s)	3251
SphI	GCATGC	1 cut(s)	4532
SsiI	CCGC	8 cut(s)	405, 1077, 2975, 3412, 3451, 4984, 5092, 5124
SspMI	CTAG	9 cut(s)	353, 390, 2465, 3252, 3660, 3747, 4215, 5007, 5195
SstI	GAGCTC	2 cut(s)	2104, 2958
StyI	CCWWGG	9 cut(s)	39, 59, 2870, 3531, 3785, 4041, 4687, 4722, 5275
TaiI	ACGT	5 cut(s)	921, 1241, 1345, 3930, 5255
TaqII	GACCGA	1 cut(s)	4775
TatI	WGTACW	6 cut(s)	206, 1850, 3196, 4575, 5197, 5405
TauI	GCSGC	3 cut(s)	408, 2978, 5095
TseFI	GTSAC	5 cut(s)	2550, 2625, 4023, 4582, 4750
Tsp45I	GTSAC	5 cut(s)	2550, 2625, 4023, 4582, 4750
TspGWI	ACGGA	2 cut(s)	2538, 2667
Van91I	CCANNNNNTGG	2 cut(s)	3289, 3454
Vha464I	CTTAAG	1 cut(s)	4615
VpaK11BI	GGWCC	8 cut(s)	558, 1516, 2046, 3445, 3805, 3867, 4002, 4556
XagI	CCTNNNNNAGG	1 cut(s)	553
XbaI	TCTAGA	1 cut(s)	352
XceI	RCATGY	5 cut(s)	1904, 2750, 4532, 4590, 4886
XcmI	CCANNNNNNNNNTGG	1 cut(s)	4762
XmiI	GTMKAC	6 cut(s)	175, 2173, 3093, 4037, 4448, 5228
XmnI	GAANNNNTTC	3 cut(s)	270, 999, 1608
XspI	CTAG	9 cut(s)	353, 390, 2465, 3252, 3660, 3747, 4215, 5007, 5195
ZraI	GACGTC	1 cut(s)	1343
ZrmI	AGTACT	3 cut(s)	3198, 4577, 5199
Zsp2I	ATGCAT	3 cut(s)	1331, 3073, 4404

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.